Digital Commons Network^™

A Gut-On-A-Chip Incorporating Human Faecal Samples And Peristalsis Predicts Responses To Immune Checkpoint Inhibitors For Melanoma, Mattia Ballerini, Serena Galiè, Punit Tyagi, Carlotta Catozzi, Hariam Raji, Amir Nabinejad, Angeli D G Macandog, Alessandro Cordiale, Bianca Ionela Slivinschi, Karol K Kugiejko, Martina Freisa, Paola Occhetta, Jennifer A Wargo, Pier F Ferrucci, Emilia Cocorocchio, Nicola Segata, Andrea Vignati, Andrey Morgun, Michela Deleidi, Teresa Manzo, Marco Rasponi, Luigi Nezi

Faculty, Staff and Student Publications

Patient responses to immune checkpoint inhibitors can be influenced by the gastrointestinal microbiome. Mouse models can be used to study microbiome-host crosstalk, yet their utility is constrained by substantial anatomical, functional, immunological and microbial differences between mice and humans. Here we show that a gut-on-a-chip system mimicking the architecture and functionality of the human intestine by including faecal microbiome and peristaltic-like movements recapitulates microbiome-host interactions and predicts responses to immune checkpoint inhibitors in patients with melanoma. The system is composed of a vascular channel seeded with human microvascular endothelial cells and an intestinal channel with intestinal organoids derived from human …

Gut Microbiota In Immuno-Oncology: A Practical Guide For Medical Oncologists With A Focus On Antibiotics Stewardship, Arielle Elkrief, Bertrand Routy, Lisa Derosa, Laura Bolte, Jennifer A Wargo, Jennifer L Mcquade, Laurence Zitvogel

Faculty, Staff and Student Publications

The gut microbiota has emerged as a critical determinant of immune checkpoint inhibitor (ICI) efficacy, resistance, and toxicity. Retrospective and prospective studies profiling the taxonomic composition of intestinal microbes of patients treated with ICI have revealed specific gut microbial signatures associated with response. By contrast, dysbiosis, which can be caused by chronic inflammatory processes (such as cancer) or comedications, is a risk factor of resistance to ICI. Recent large-scale meta-analyses have confirmed that antibiotic (ATB) use before or during ICI therapy alters the microbiota repertoire and significantly shortens overall survival, even after adjusting for prognostic factors. These results underscore the …

Bispecific Antibodies In Hematologic And Solid Tumors: Current Landscape And Therapeutic Advances, Lorenzo Guidi, Julian Etessami, Carmine Valenza, Augusto Valdivia, Funda Meric-Bernstam, Enriqueta Felip, Giuseppe Curigliano

Faculty, Staff and Student Publications

Bispecific antibodies (bsAbs) have emerged as a novel class of therapeutics, offering a dual-targeting strategy to enhance the therapeutic efficacy of monoclonal antibodies, which is often limited by tumor heterogeneity and the occurrence of resistance mechanisms. By simultaneously engaging two distinct antigens or pathways, bsAbs disrupt multiple signaling cascades simultaneously, preventing escape mechanisms and offering a more durable response. Furthermore, they can optimize immune activation, improving immune cell recruitment strategies. In particular, T-cell engager bsAbs facilitate immune cell-mediated tumor destruction by linking T cells to tumor antigens. Instead, dual immune checkpoint inhibitors (CPIs) enhance immune activation by blocking inhibitory signals. …

Cll Cell-Derived Exosomes Alter The Immune And Hematopoietic Systems, Ivo Veletic, David M Harris, Uri Rozovski, Maria Teresa S Bertilaccio, George A Calin, Koichi Takahashi, Ping Li, Zhiming Liu, Taghi Manshouri, Rares-Constantin Drula, Ken Furudate, Muharrem Muftuoglu, Anwar Hossain, William G Wierda, Michael J Keating, Zeev Estrov

Faculty, Staff and Student Publications

The origins of immunosuppression, neutropenia, and anemia in patients with chronic lymphocytic leukemia (CLL) are not fully understood. Because in patients with CLL, circulating exosomes, which participate in cell-to-cell interactions, are CLL cell-derived, we examined whether those exosomes contribute to abnormal features of this disease. Our data revealed that CLL cell-derived exosomes engulfed by healthy donors’ monocytes, fibrocytes, and lymphocytes altered target-cell gene and protein expression and suppressed normal hematopoiesis. CLL cell-derived exosomes increased normal monocytes’ CD14 and CD16 expression such that it mimicked the accessory cell profile and upregulated T cells’ checkpoint PD-1 and CD160 protein levels, potentially reducing …

In Vitro Evaluation Of A Novel Computationally Designed Anticancer Peptide In Ovarian Cancer Cell Models, Basma A. Shaala

Theses and Dissertations

Ovarian cancer remains one of the leading causes of cancer-related mortality among

women, highlighting the need for novel therapeutic agents with improved selectivity and

reduced toxicity. Anticancer peptides (ACPs) have emerged as promising candidates

due to their ability to selectively target cancer cells. In this study, the anticancer activity

of a computationally designed ACP was evaluated in vitro using the SKOV-3 ovarian

cancer cell line, with HEK cells employed as a non-cancerous control.

Cell viability was assessed using the MTT assay, and cytotoxicity was further confirmed

by the trypan blue exclusion method. Apoptosis and necrosis were analyzed using

Annexin V-FITC/Propidium …

Event-Based Eye Tracking: Event-Based Vision Workshop 2025, Qinyu Chen, Et. Al.

Research Collection School Of Computing and Information Systems

No abstract provided.

Evaluation Of Nature-Based Engineering Stream Restoration Of Plaster Creek Through Leisure Creek Condominium Association, Julie Wildschut, Rebecca Evenhouse, Andrea Lubberts, Dave Warners, Haley Weesies

Faculty and Professional Research

When land that was once covered with vegetation and soil becomes dominated by impervious concrete and rooftops, the amount of water infiltrated by the ground plumets. As a result, most suburban areas contribute to the impairment of streams by adding excess water, nutrients, and pollutants to nearby waterways each time it rains. One harmful result of this is the erosion of stream banks. The rapid influx of water that occurs after rain events can destabilize and erode stream banks, resulting in excess sediment in the water. One location where this happened very prominently was a section of Plaster Creek which …

Understanding Parents’ Beliefs About The Etiology Of Youth Mental Health Problems: Differences Across Race/Ethnicity, Madeline Buchanan, Lindsay E. Holly

Psychology Faculty Research and Publications

Parents’ etiological explanations for youth mental health problems are relatively understudied; most studies focus on adult beliefs about the causes of mental disorders experienced by adults. This is concerning given research demonstrating that parents’ causal beliefs may influence several aspects of youth mental health service utilization including whether parents seek help for their youth and how they engage in treatment. The current study used a novel methodology to examine causal beliefs about youth mental health problems among a diverse group of parents (N = 417; 36.9% non-Hispanic White; 33.3% Black/African American; 29.8% Hispanic/Latino) who completed a series of questionnaires …

A Vacuolar Proton Pump Controls The Post-Germinative Growth Of Rice (Oryza Sativa Ssp. Japonica), Peter James Icalia Gann, Soumen Nandy, Rodrigo Botelho, Kari Vinzant, Mariya Khodakovskaya, Vibha Srivastava

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Post-germinative growth in plants is controlled by sucrose mobilization from the endosperm. Regulation of this process, however, is not fully understood. Transcriptional suppression of the rice vacuolar H+ translocating pyrophosphatase (V-PPase) referred to as VPP5 led to enhanced starch content in mature grains. However, vpp5 suppressed lines displayed a slower post-germinative growth rescuable by sucrose, indicating inefficient sucrose mobilization from the endosperm. Biochemical and gene expression analyses showed a slower rate of starch to sucrose conversion and altered expression of the key starch utilization pathway genes. We hypothesized that the modulation of metabolic activities in the vpp5 lines was based …

Predictors Of Nursing Students' Stress, Anxiety, And Depression During The Covid-19 Pandemic In A Hispanic-Serving University In South Texas: A Cross-Sectional Study, Maria I. Diaz, Eleftherios Gkioulekas, Nancy Nadeau

School of Mathematical & Statistical Sciences Faculty Publications

Background: In nursing education, there have been several studies on the impact of the COVID-19 pandemic on the ability of nursing students to cope while in nursing school.

Purpose statement: The goal of this study is to assess undergraduate nursing students' support mechanisms as predictors of stress, anxiety, and depression during the COVID-19 pandemic within a Hispanic-serving institution in South Texas.

Methods: Across-sectional design was used in this study. An online survey using self-reported questionnaires was used to gather data from an undergraduate nursing student cohort during the Fall 2021 semester. Linear regression was used to identify the predictors of …

The Density Of Virgin, Consumer And Environmental Plastics: An Investigation Using Gas Displacement Pycnometry, Andrew Turner, Katie Jones, Montserrat Filella

School of Geography, Earth and Environmental Sciences

Density is a fundamental property of plastics and is particularly significant in determining the transport and fate of waste plastics that enter aquatic systems. However, densities are rarely determined in the environmental literature and values employed for modelling or impacts are often unsourced or derived from secondary databases. In this study, we employ helium displacement pycnometry to determine the skeletal densities of non-porous plastics whose polymer composition had been established from manufacturer's data, resin codes or Fourier transform infrared spectrometry. Two independent, collaborative laboratories, providing measurements within 3.5 % of each other and with precisions of <1 % (as relative standard deviation), analysed a total of 42 virgin, consumer and environmental plastics consisting of ten common polymer types. Measured densities of plastics in all categories, and most notably for polybutylene terephthalate, polyethylene terephthalate, polypropylene and polyvinyl chloride, were often outside the ranges reported by a comprehensive online resource. Possible reasons for discrepancies include the occurrence of dense additives (evaluated by X-ray fluorescence analysis), the presence of inaccessible microscopic pores below a laminated surface, contamination of the main polymer by a secondary one, and structural changes on weathering. Regardless of precise causes, most results suggest that individual polymers have a broader range of densities than is generally published or considered in the literature. Accordingly, and in particular where buoyancy is critical, more precise, sample-specific measurements are recommended.

A Holistic Approach Is Needed For The Treatment Of Alcohol Use Disorder, Victoria T. Kronsten, Ashwin Dhanda

Peninsula Medical School

EditorialsA holistic approach is needed for the treatment of alcohol use disorderKronsten, Victoria T.1; Dhanda, Ashwin2Author Information1Faculty of Life Sciences and Medicine, School of Immunology and Microbial Sciences, Roger Williams Institute of Liver Studies, King’s College London, London, UK2Faculty of Health, School of Immunology and Microbial Sciences, University of Plymouth, Plymouth, UKAbbreviations: AUD, alcohol use disorder; AUDIT-C, Alcohol Use Disorders Identification Test— Consumption; EtG, ethyl glucuronide.Correspondence Victoria T. Kronsten, Faculty of Life Sciences and Medicine, School of Immunology and Microbial Sciences, Roger Williams Institute of Liver Studies, King’s College London, London, UK. Email: [email protected] Transplantation 31(6):p 699-700, June 2025. | …

Innovations In Intestinal Organoid Technology Featuring An Open Apical Surface, Ye Chen, Yi Wang

2020-Current year OA Pubs

Since the development of the three-dimensional (3D) "mini-gut" culture system, adult stem cell-derived organoid technology has rapidly advanced, providing in vitro models that replicate key cellular, molecular, and physiological properties of multiple organs. The 3D intestinal organoid system has resolved many long-standing challenges associated with immortalized or cancer cell cultures, offering unparalleled capabilities for modeling gastrointestinal development and diseases. However, significant limitations remain, including restricted accessibility to the epithelial apical surface for studying host-microbe interactions, interruptions in modeling chronic gastrointestinal diseases due to frequent passaging and dissociation, and the absence of mechanical cues such as peristalsis and luminal flow, which …

Rna Interference In Protozoan Parasites And Its Application, Lon-Fye Lye, Deborah E Dobson, Stephen M Beverley, Min-Che Tung

2020-Current year OA Pubs

RNA interference (RNAi) is a biological process in which RNA molecules are involved in sequence-specific suppression of gene expression, via small RNA triggers derived from double-stranded RNA that can target specific genes; it is a natural process that plays a role in both the regulation of protein synthesis and in immunity. Discovery of RNAi by Fire and Mello in 1998 had a profound impact on unraveling novel aspects of eukaryotic biology. RNA interference (RNAi) has proven to be an immensely useful tool for studying gene function and validation of potential drug targets in almost all organisms. A great advance in …

Polyclonal Plasma Cell (Polypc) Signature As A Key Indicator For Predicting The Progression Of Mgus To Multiple Myeloma, Fumou Sun, Yan Cheng, Catherine Ma, Hongwei Xu, Clyde Bailey, David Mery, Timothy Cody Ashby, Daisy Alapat, Yong Li, Ken H Young, Samer Al Hadidi, Sharmilan Thanendrarajan, Carolina Schinke, Maurizio Zangari, Frits Van Rhee, Guido Tricot, John D Shaughnessy, Fenghuang Zhan

Faculty, Staff and Students Publications

Background

Multiple myeloma (MM) is virtually always preceded by monoclonal gammopathy of undetermined significance (MGUS). Elevated serum markers are used to classify MGUS patients into clinical risk categories. Previous research has indicated that the absence of a normal plasma cell signature in MGUS is linked to early progression.

Objective

To confirm that the presence of a “polyclonal plasma cell (PolyPC) signature” serves as a robust negative predictor of MGUS progression.

Methods

374 MGUS patients were enrolled, including 334 patients with stable disease and 40 patients who progressed to MM within 10 years. An oligonucleotide microarray analysis was performed on mRNA …

All Genetic Subtypes Of B-Cell Acute Lymphoblastic Leukemia Exhibit Increased Incidence Rates In Children With Down Syndrome, Ching-Ju Hsu, Jeremy M Schraw, Tania A Desrosiers, Amanda E Janitz, Russell S Kirby, Eirini Nestoridi, Wendy N Nembhard, Jason L Salemi, Charles Shumate, Jean Paul Tanner, Mahsa M Yazdy, Michael E Scheurer, Karen R Rabin, Philip J Lupo

Faculty, Staff and Students Publications

No abstract provided.

Graphage: Unleashing The Power Of Graph Neural Network To Decode Epigenetic Aging, Saleh Sakib Ahmed, Nahian Shabab, Abul Hassan Samee, M Sohel Rahman

Faculty, Staff and Students Publications

DNA methylation is a crucial epigenetic marker used in various clocks to predict epigenetic age. However, many existing clocks fail to account for crucial information about CpG sites and their interrelationships, such as co-methylation patterns. We present a novel approach to represent methylation data as a graph, using methylation values and relevant information about CpG sites as nodes, and relationships like co-methylation, same gene, and same chromosome as edges. We then use a graph neural network (GNN) to predict age. Thus our model, GraphAge leverages both the structural and positional information for prediction as well as better interpretation. Although, we …

Burst Steroid Therapy And Quality Of Life In Patients With Acute Heart Failure: Insights From The Cortahf Trial, Matteo Pagnesi, Douglas L Mann, Et Al.

2020-Current year OA Pubs

AIMS: Patients hospitalized with acute heart failure (AHF) treated with a 7 day prednisone course in the CORTAHF pilot trial had a greater improvement in health-related quality of life (QoL) at Day 7 in both the overall population and in patients with baseline interleukin 6 > 13 pg/mL. This post-hoc analysis examines the specific QoL domains and the relationship between clinical signs of congestion and QoL.

METHODS: In the CORTAHF pilot trial, patients with AHF and high-sensitivity C-reactive protein (hsCRP) > 20 mg/L were randomized 1:1 to once-daily oral 40 mg prednisone for 7 days plus usual care or usual care alone. …

Claustrum Volume Is Reduced In Multiple Sclerosis And Predicts Disability, Nicole Shelley, Bradley Judge, Dmitriy A Yablonskiy, Robert T Naismith, Matthew R Brier, Anne H Cross

2020-Current year OA Pubs

OBJECTIVE: The claustrum is a small, thin structure of predominantly gray matter with broad connectivity and enigmatic function. Little is known regarding the impact of claustrum pathology in multiple sclerosis (MS).

METHODS: This study assessed whether claustrum volume was reduced in MS and whether reductions were associated with specific disability domains. We compared the impact of claustrum volume reductions on neurological disability versus the well-studied thalamus. Data from the 15,793 participants in the MS PATHS study were used for primary analyses. A second cohort of 71 people with MS and healthy controls from our center was used for validation.

RESULTS: …

Nf2 Loss-Of-Function And Hypoxia Drive Radiation Resistance In Grade 2 Meningiomas, Bhuvic Patel, Sangami Pugazenthi, Shree S Pari, Tatenda Mahlokozera, William A Leidig, Hsiang-Chih Lu, Alicia Yang, Kaleigh Roberts, Patrick Desouza, Kyle P Mcgeehan, Diane D Mao, Namita Sinha, Joseph E Ippolito, Sonika Dahiya, Hiroko Yano, Albert H Kim, Et Al.

2020-Current year OA Pubs

BACKGROUND: World Health Organization Grade 2 meningiomas (G2Ms) often recur and resist therapies. Grade 2 meningiomas with histopathological necrosis have been associated with worse local control (LC) after radiation therapy, but the drivers and biomarkers of radiation resistance in G2Ms remain unknown.

METHODS: We performed genetic sequencing and histopathological analysis of 113 G2Ms and investigated the role of genetic and microenvironmental factors on clonogenic survival after ionizing radiation. We performed transcriptional profiling of our in vitro model and 18 human G2M tumors by bulk RNA sequencing as well as 8 G2Ms by single nuclei RNA sequencing.

RESULTS: NF2 loss-of-function (LOF) …

Evaluating Artificial Intelligence Chatbots For Patient Education In Oral And Maxillofacial Radiology, Dilek Helvacioglu-Yigit, Husniye Demirturk, Kamran Ali, Dania Tamimi, Lisa J. Koenig, Abeer Almashraqi

School of Dentistry Faculty Research and Publications

Objective

This study aimed to compare the quality and readability of the responses generated by 3 publicly available artificial intelligence (AI) chatbots in answering frequently asked questions (FAQs) related to Oral and Maxillofacial Radiology (OMR) to assess their suitability for patient education.

Study Design

Fifteen OMR-related questions were selected from professional patient information websites. These questions were posed to ChatGPT-3.5 by OpenAI, Gemini 1.5 Pro by Google, and Copilot by Microsoft to generate responses. Three board-certified OMR specialists evaluated the responses regarding scientific adequacy, ease of understanding, and overall reader satisfaction. Readability was assessed using the Flesch-Kincaid Grade Level (FKGL) …

Understanding Intergenerational Trauma Among Arab, Middle Eastern, And North African (Arab/Mena) Americans, Rawan H. Atari-Khan, Nuha Alshabani, S. Dolma Rabgay, Farida Refaat Elhedk, Ava Clifford, Sofia Gomariz, Warda Sahib

Psychology Faculty Research and Publications

Arab, Middle Eastern, and North African Americans experience various forms of racial–ethnic trauma in the United States, including historical and intergenerational trauma (IGT). The population has been migrating to the United States since the 1800s, and the number of migrants has risen in recent decades due to war, persecution, and family reunification. As a result, the children of these migrants may be exposed to IGT, with research suggesting that descendants of individuals who have experienced trauma are more susceptible to poor mental health outcomes. Using semistructured interviews, alongside the art of storytelling and empowerment through critical consciousness, this study investigated …

Understanding The Habitat Associations Of Nearshore Rockfishes Inside And Outside Of Marine Protected Areas Using Hook-And-Line Data, Kaila Raeanne Fritch

Master's Theses

Habitat is an important driver in shaping the distribution of a range of species. For demersal fish species, benthic habitat characteristics can drive distribution and community structure. Understanding the relationship between habitat and species distribution in important, especially for exploited species such as rockfishes (Sebastes spp.). Increased data on the relationship between rockfish and benthic habitat characteristics could enhance marine protected areas (MPAs) and management practices which are used to protect rockfishes along the U.S. West Coast. To address this, we used hook-and-line, catch-and-release data that were collected inside two MPAs on the central coast of California, and …

Filling Gaps In Scientific Data Sets Using Physics Informed Neural Networks: A Case Study In Velocity Fields, Ellen Saunders

Master's Theses

Gaps in scientific data sets are a persistent issue for researchers in a variety of fields, and while nothing makes up for missing out on real data, well-simulated synthetic data can be a useful tool. In the world of image processing, machine learning techniques have become quite sophisticated at taking an image with a missing component and filling in that space with something believable. The aim of this thesis is to take machine learning techniques similar to what gets used in image processing and repurpose them to infill gaps in scientific data sets in a realistic manner. This thesis compares …

Optimization Of Downy Mildew Management And Baby Kale Production On The Central Coast Of California, Emily M. Locke-Paddon

Master's Theses

Baby kale (Brassica oleracea and other species) is a vital commodity as a salad green in spring mix and is widely produced on the Central Coast of California. Downy mildew, caused by Hyaloperonospora brassicae, is a devastating disease infecting economically important crops like cabbage, broccoli, cauliflower, horseradish, rapeseed, kohlrabi, Brussels sprouts, and kale. This foliar disease causes plant tissue to exhibit chlorotic and necrotic flecking and gray-white sporulation, affecting both the yield and quality of the crop and rendering the affected leaves unmarketable. The Central Coast’s cool climate and nighttime moisture, combined with dense plantings and sprinkler irrigation, …

Using Hydrologic And Hydraulic Simulations To Predict Near-Term Effects Of Beaver Habitat Restoration On Flood Flows, Luke S. Stewart

Master's Theses

There is a growing need to predict the complex hydrologic and hydraulic effects of beaver-maintained wetlands. This study uses relatively simple inputs and a replicable methodology to predict flood flow impacts of beaver habitat restoration at the site design scale using the engineering industry’s customary hydrology and hydraulic modeling approach. This study focuses on Toro Creek, the primary drainage for a 15.2 square mile watershed on the California Central Coast.

A hydrologic model of Toro Creek watershed was developed in HEC-HMS to approximate saturated soil conditions typical of late-spring flooding events observed in the region. The hydrologic model was calibrated …

Vertical And Latitudinal Variability Of Marine Heatwaves In The California Current, Gavin M. Plume

Master's Theses

Marine heatwaves (MHWs) are increasing in frequency and intensity globally, with grave effects on marine ecosystems and their dependent industries. There are significant gaps in knowledge of the subsurface behavior and geographic distribution of MHWs, especially in eastern boundary upwelling systems like the California Current. Here, two decades of full water-column temperature observations from a shallow autonomous profiler in San Luis Obispo Bay (~10m) revealed that while MHWs have similar average durations and intensities across all depths, one-third of bottom MHWs occur without a concurrent surface signal. MHWs across the water column initiated during anomalously weak upwelling or downwelling conditions …

The Northern Elephant Seals (Mirounga Angustirostris) Of The Piedras Blancas Colony: A Story Of Recolonization, Monitoring And Movement, Mackenzie Davidson

Master's Theses

Northern elephant seals (Mirounga angustirostris) are large, ocean migrating carnivores that have a wide distribution along the west coast of North America. Like other pinniped species, northern elephant seals spend considerable time out at sea foraging and return to land to molt and breed. Northern elephant seals were hunted to near extinction in the late 1800s. Due to conservation efforts they have since rebounded, and they currently occupy 27 known terrestrial breeding colonies. Much literature has documented the recolonization, population dynamics, and dispersion of this species. Currently, Piedras Blancas (San Simeon, California) is the largest mainland breeding colony of northern …

Identifying Interchromosomal Interactions Using A Graph-Based Computational Pipeline, Ethan Outangoun

Master's Theses

Current computational tools for analyzing chromatin organization are mainly focused on intrachromosomal interactions, despite growing evidence that suggests long-range interactions across chromosomes contribute to transcriptional regulation and disease development. This thesis aims to address this gap in interchromosomal genome analysis, presenting a robust computational pipeline that identifies a clique (i.e., a subgraph) of highly interacting trans-chromosomal regions anchored at a user-specified seed genomic locus. A weighted interaction network is constructed from an input Hi-C contact matrix, a widely used experimental assay for measuring genome-wide chromatin interactions. We model this input contact matrix as a graph and devise three different strategies …

Myceli-Yum: Elucidating Structure-Property Relationships For Polymer Degradation By Mycelial Digestion, Jordan Scott Ford

Master's Theses

Since the industrial entrance of polymer plastic materials, plastic has become ubiquitous in both everyday use and waste. Due to inefficiencies and knowledge gaps, current recycling methods are not able to account for the high scale of plastic waste, resulting in the bulk of this waste being landfilled, mishandled, and deposited in the environment. Mycelium, the microorganism responsible for fruiting mushroom bodies and mold growth, holds potential to reduce plastic waste and can potentially be utilized as a method of industrial recycling. Following a drug-design approach, the active site of mycelial enzymes responsible for natural biopolymer degradation have been assessed …