Digital Commons Network^™

Investigating The Role Of Early Growth Response 1 (Egr1) Gene In Riluzole-Induced Apoptosis In Osteosarcoma, Syeda Maryam Azeem

Dissertations, Theses, and Capstone Projects

Osteosarcoma is a rare but aggressive bone malignancy most commonly affecting adolescents and young adults (AYA). With 5-year survival rate stagnating over the last four decades despite the advancements in multi-modal therapy, drug repurposing offers a rapid path to new osteosarcoma treatments. Recent studies suggest that Riluzole, a drug approved for amyotrophic lateral sclerosis (ALS), may be repurposed for osteosarcoma treatment due to its ability to slow tumor progression and induce apoptosis. Previously, we have shown that Riluzole increases reactive oxygen species (ROS), activating c-Abl, which phosphorylates YAP in the nucleus. YAP then forms a complex with p73 to enhance …

Behavioral Biomarkers Of Vulnerability In The Aba Model, Nora Ozer

Dissertations, Theses, and Capstone Projects

Anorexia nervosa (AN) is a severe psychiatric disorder with the highest mortality rate among mental health conditions, yet effective biological treatments remain limited. The activity-based anorexia (ABA) paradigm, which combines food restriction with wheel access to model the hyperactivity and self-starvation cycle characteristic of AN, shows considerable individual variability in vulnerability. Understanding what predisposes certain individuals to develop the full ABA phenotype while others remain resilient could inform early intervention strategies for human AN. This study employed continuous behavioral monitoring technology to identify early predictors of vulnerability to activity-based anorexia in mice, with the goal of discovering behavioral biomarkers that …

The Role Of Parent Psychiatric Symptoms In The Intergenerational Continuity Of Child Neglect: A Prospective Examination, Cody D. Stitzel

Dissertations, Theses, and Capstone Projects

Parental history of childhood neglect is a risk factor for offspring neglect. When a parent and their offspring both experience neglect in childhood, the phenomenon is referred to as the intergenerational continuity of child neglect. Understanding how child neglect is continued from one generation to the next is essential for creating effective interventions and prevention strategies that may help break the cycle. Since links between childhood neglect and heightened risk for various psychiatric symptoms have consistently been identified in research, as well as links between various psychiatric symptoms and heightened risk for child neglect, it may be that parents’ psychiatric …

Wavelength And Luminance Interaction On Spatio-Temporal Contrast Sensitivity Function, Taylan S. Ergun

Dissertations, Theses, and Capstone Projects

Contrast sensitivity (CS) measures the ability to discriminate objects from their backgrounds under varying conditions(Campbell & Robson, 1968). Contrast sensitivity is important for navigating the real world and operating under different light conditions. The contrast sensitivity function (CSF) reflects how the visual system performs under different conditions. This measure is crucial in assessing the health of the visual system (Chen & Gardner, 2021). CSF contributes to a deeper understanding of how the visual system processes and integrates properties of light to create visual sensation and perception. Human contrast sensitivity depends on the viewing conditions such as field size, luminance, color, …

Liberated Learning Spaces: A Visual Playlist, Tarilyn R. Little

Dissertations, Theses, and Capstone Projects

Liberation can be defined as “the act of setting free” or “being free from limits on thoughts and behavior.” Both an action and a state of being, liberation has long been centered as a key function of education, particularly for those who live in historically and systemically marginalized identities. Despite the long-standing existence of liberation-focused pedagogies and activism aimed at transforming schools and students’ lived experiences, educational institutions in the United States have struggled to live out these ideals, often sustaining and replicating systems and practices of oppression. Drawing on historical and contemporary conversations in education and psychology, Liberated Learning …

The Role Of Water In Peptide Behavior And Peptide-Based Water-Responsive Crystals, Janel Jannette Rivera Cancel

Dissertations, Theses, and Capstone Projects

Water plays a crucial role in shaping the behavior and function of biomolecules across the liquid, supercooled, and glassy states. This thesis presents a dual approach combining computational simulations and experimental investigations to explore the influence of water on biomolecular systems, particularly peptide-based materials. We employ molecular dynamics simulations to study the hydration-dependent structural dynamics of homopolymer peptides. Simulations were carried out across a range of temperatures and pressures to analyze changes in hydration and structural stability, shedding light on the behavior of peptides under supercooled and glassy conditions. These studies are especially relevant for understanding biomolecular stability in cryopreservation. …

Improving The Assessment Of Functional Ability In Older Adults, Aneela Rahman

Dissertations, Theses, and Capstone Projects

Deficits in instrumental activities of daily living (IADL) are a hallmark of dementia, and the unprecedented aging of the population strongly motivates early identification of older adults at-risk for functional decline. In 2015, the White House Conference on Aging emphasized the need to improve detection of diminished capacity across important areas of functioning. To date, there is very limited research on early IADL changes in cognitively unimpaired (CU) individuals and those with subjective cognitive decline (SCD) and mild cognitive impairment (MCI), who may be in early preclinical dementia stages. Additionally, little is known about how changes in functional brain networks …

Nurses’ Knowledge About Assisted Reproductive Methods And Their Awareness About It In Potential Immune-Related Outcomes Of Conceived Children, Salma Abd-Elatty Moawed, Abdallah Ahmed Said

Nursing

Abstract A rising worldwide health concern, infertility is impacted by a number of environmental and lifestyle factors. With cutting-edge methods like intrauterine insemination, intracytoplasmic sperm injection, and in vitro fertilization, assisted reproductive technology gives infertile couples hope. Because they provide clinical care, education, and emotional support before, during, and after assisted reproductive technology procedures, nurses are essential to its success. The purpose of this study is to assess nurses’ knowledge and awareness about assisted reproductive methods and the immune outcomes among the resulting children. A structured interview questionnaire will be used to gather data from 130 nurses as part of …

Single Nuclei Transcriptomics Reveals Cellular Diversity In Tsc Subependymal Giant Cell Astrocytomas, Jennie C. Holmberg, Vijay Shankar, Rachel A. Lyman, Trudy F.C. Mackay, David M. Feliciano

Publications

Tuberous sclerosis complex (TSC) is a genetic disorder characterized by benign growths called hamartomas that are a significant cause of morbidity and mortality. Hamartomas are found along the neurocutaneous axis including along the brain’s ventricles near the boundaries of the striatum. They can be categorized by size and include small subependymal nodules (SENs) or larger subependymal giant cell astrocytomas (SEGAs). Here, we describe a quantitative analysis of SEGA cell identities based on single nuclei RNA sequencing. SEGAs contain several cell types. In contrast to unaffected samples, SEGAs have pronounced vasculature, more endothelial cells, increased perivascular macrophages, less myelination, and altered …

Integrated Systems Biology Identifies Disruptions In Mitochondrial Function And Metabolism As Key Contributors To Hfpef, Andrew A. Gibb, Kyle Lapenna, Ryan B. Gaspar, Nadina R. Latchman, Yinfei Tan, Carmen Choya-Foces, Jake E. Doiron, Zhen Li, Huijing Xia, Michael P. Lazaropoulos, Mariell Conwell, Thomas E. Sharp, Traci T. Goodchild, David J. Lefer, John W. Elrod

School of Graduate Studies Faculty Publications

Heart failure with preserved ejection fraction (HFpEF) accounts for ∼50% of HF cases. The ZSF1-obese rat model recapitulates clinical features of HFpEF including hypertension, obesity, metabolic syndrome, exercise intolerance, and diastolic dysfunction. We utilized a systems-biology approach to define the metabolic and transcriptional signatures to gain mechanistic insight into pathways contributing to HFpEF development. Male ZSF1-obese, ZSF1-lean hypertensive controls, and WKY (wild-type) controls were compared at 14 weeks of age for extensive physiological phenotyping and left ventricle (LV) tissue harvesting for unbiased-metabolomics, RNA-sequencing, and mitochondrial morphology and function. Utilizing ZSF1-lean and WKY controls enabled a distinction between hypertension-driven molecular changes …

Trends In Genitourinary Tumors: Academic Center Experience Over 16 Years, Samah N. Saharti, Fawaz M. Almutairi

Saudi Medical Journal

ABSTRACT Objectives: To analyze the trends, histopathological characteristics, and demographic distribution of genitourinary (GU) tumors at a major academic center in Jeddah, Saudi Arabia, over a 16-year period (2007–2023). Methods: A retrospective review of electronic medical records was conducted, including all histologically confirmed cases of GU tumors. Data on patient demographics, tumor type, and histopathological findings were extracted and analyzed using descriptive and inferential statistical methods. Results: A total of 1,791 GU tumor cases were identified. Bladder cancer was the most prevalent (42%), followed by prostate cancer (21%) and renal cell carcinoma (21%). Testicular and ureteral tumors were less common, …

Unmasking Iron Deficiency And Iron Deficiency Anemia In Saudi Arabia: Data From A Large Private Sector Lab, Osman O. Radhwi, Omar M. Raslan, Eman M. Mansory

Saudi Medical Journal

ABSTRACT Objectives: This study investigates the prevalence of iron deficiency anemia (IDA) in Saudi Arabia, analyzing variations by gender, age, and geographical region. Methods: An observational, cross-sectional analysis was conducted from January 2014 to February 2024 using data from Al Borg laboratories, including ferritin levels, demographics, and routine hematological results. Results: The study included 420,956 individuals, with 73% being female and 94% over 15 years old. The IDA was found in 19.8% of patients, Non-Anemic Iron Deficiency (NAID) in 23.7%, and other anemias in 9.6%. Females represented 93% of those with IDA and NAID. All patients under 12 were anemic, …

Serum Levels Of Gdf-15, Betatrophin, Tnf-Α And Il-6 In Diabetic Patients With And Without Diabetic Complications, Ahmet Yılmaz, Tugce Kaymaz, Burkay Yakar, Mehtap Gömleksiz, Erhan Önalan, Mehmet F. Gürsu

Saudi Medical Journal

ABSTRACT Objectives: To investigate serum levels of growth differentiation factor 15 (GDF-15), inflammatory biomarkers, and betatrophin in individuals with diabetes mellitus, both with and without complications. Methods: A cross-sectional analysis was conducted involving patients with diabetes. Participants were categorized into 3 groups: Group 1 (30 healthy individuals without diabetes), Group 2 (30 diabetic patients without complications), and Group 3 (30 diabetic patients with complications). Blood samples were collected to assess standard biochemical parameters, as well as plasma concentrations of betatrophin, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and GDF-15. All serum biomarkers were quantified using enzyme-linked immunosorbent assay (ELISA) techniques. Results: …

Cutting-Edge Research From The 2024 Middle East Rheumatology Conference (Mrc 2024) Of The Gulf Cooperation Council Association Of Immunology And Rheumatology (Gcc-Air), Ahmed S Zayat, Beena Hamid, Martin Lee, Fahdah Alokaily, Maryam Al Nisf, Mahmood Hachim, Hanan Al Reyes, Khalid Alnaqbi, Amal Hussain, Fiaz Alam, Ghida Al Dabi, Suad Hannawi

Saudi Medical Journal

No abstract provided.

Use Of Manga And Videos As Trigger Scenarios In Interprofessional Education For Undergraduate Medical And Nursing Students, Yukihiro Ike, Yoshiyuki Mitsui, Akiko Tanoue

Journal of Medical Education

Background: Interprofessional education (IPE) is being implemented in medical schools worldwide. Role-play, group discussion, and simulation have been among the educational methods reported, and the eff ects of each educational method have been examined. Objective: In IPE at the Faculty of Medicine and School of Nursing, we studied the use of manga and videos as trigger scenarios for group work. Methods: IPE involving manga and videos implemented in an online virtual setting. Participants filled out a questionnaire before and after IPE. Results: After IPE, students gave positive feedback about the use of manga and videos as teaching materials. Students seemed …

Memory-Efficient Graph Processing On Gpus: Reducing Intermediate Data Structure Overhead, Chang Ye

Dissertations and Theses Collection (Open Access)

The increasing scale of real-world graphs in domains such as fraud detection, community detection, and biological analysis demands high-throughput, memory-efficient graph processing solutions. GPUs offer massive parallelism for accelerating such workloads, and numerous frameworks have been developed to leverage their computational power. These frameworks primarily focus on optimizing scheduling to better align graph processing with GPU architectures. It performs well for algorithms with low memory demands, such as BFS, SSSP, and PageRank. However, for algorithms that require substantial memory, such as label propagation, and subgraph counting, the limited memory capacity of GPUs often becomes a significant bottleneck.

This dissertation addresses …

Computational Analysis Of Dna Methylation From Long-Read Sequencing, Yilei Fu, Winston Timp, Fritz J Sedlazeck

Faculty, Staff and Students Publications

DNA methylation is a critical epigenetic mechanism in numerous biological processes, including gene regulation, development, ageing and the onset of various diseases such as cancer. Studies of methylation are increasingly using single-molecule long-read sequencing technologies to simultaneously measure epigenetic states such as DNA methylation with genomic variation. These long-read data sets have spurred the continuous development of advanced computational methods to gain insights into the roles of methylation in regulating chromatin structure and gene regulation. In this Review, we discuss the computational methods for calling methylation signals, contrasting methylation between samples, analysing cell-type diversity and gaining additional genomic insights, and …

Validation Of An Assay For Ngal In A Pediatric Population, Nazmin Bithi, Ridwan B Ibrahim, Estella L Tam, Radwa Almamoun, Annette C Frenk Oquendo, Ayse Akcan-Arikan, Sridevi Devaraj

Faculty, Staff and Students Publications

Background and objectives: Acute kidney injury (AKI) poses a serious clinical challenge, particularly in high-risk environments, due to its association with increased morbidity and mortality. Traditional diagnostic markers, such as serum creatinine, often detect AKI only after significant kidney damage has occurred, limiting opportunities for early intervention. Neutrophil gelatinase-associated lipocalin (NGAL) has emerged as a promising early biomarker due to its rapid upregulation following kidney ischemia. NGAL supports renal recovery by reducing toxicity and promoting tubular regeneration via heme oxygenase-1 activity. This study aimed to validate the BioPorto ProNephro AKI™ turbidimetric immunoassay for urinary NGAL on the Ortho Vitros XT7600 …

High-Fidelity Finite Element Modeling Technique To Improve Sensitivity To Bone Tissue Changes Of Older Adults With Obesity Undergoing Intensive Lifestyle Intervention, Michael A K Liebschner, Daeseung Kim, Nina Klonis, Giulia Gregori, Reina Armamento-Villareal, Clifford Qualls, Dennis T Villareal

Faculty, Staff and Students Publications

Introduction: Obesity presents a significant health risk for the aging population. Research shows that weight loss and regular exercise can greatly improve the functional status of older adults who are obese. However, weight loss may also result in a decrease in bone mass. To properly assess changes in fracture risk due to lifestyle interventions, a direct biomechanical evaluation of bone strength and fracture risk at metabolically active sites is essential.

Methods: Computed tomography scans taken at two different time points of ten human volunteers provided the foundation for this study. A high-fidelity segmentation and modeling approach was taken to generate …

Optical Coherence Tomography Enables The Depth-Resolved Measurement Of Cilia Beat Frequency In Ex Vivo Human Fallopian Tubes, Dilara J Long, Tian Xia, Photini F Rice, Deirdre M Scully, Makayla Johnson, Ryan Mistifer, Andrea Aguirre, John M Heusinkveld, Irina V Larina, Jennifer K Barton

Faculty, Staff and Students Publications

Objectives: The movement of cilia in the fallopian tubes (FTs) facilitates important processes involved in fertility, and abnormalities in cilia function are linked with diseases including endometriosis and pelvic inflammatory disease. For the first time, we demonstrate the use of optical coherence tomography (OCT) to create depth-resolved mapping of motile cilia locations and quantify cilia beat frequency (CBF) in human FT samples ex vivo.

Methods: Segments of the FT ampulla were acquired from five patients following salpingectomy under an IRB approved protocol. The samples were longitudinally opened to expose the luminal surface for imaging. A sequence of at least 500 …

Patient-Centered Management Of Heavy Menstrual Bleeding While On Anticoagulation: A Survey Study, Benjamin Barnett, Rushad Patell, Dana Angelini, Leben Tefera, Jordan K Schaefer, Ang Li, Alejandra Gutierrez Bernal, Shruti Chaturvedi, Anjlee Mahajan, Leslie Lake, Jori May, Anna L Parks

Faculty, Staff and Students Publications

Background: Nearly three-quarters of women experience heavy menstrual bleeding (HMB) during anticoagulation treatment for venous thromboembolism (VTE). HMB can lead to medical interventions, impact school or work and diminish quality of life (QOL). While there are several management options for HMB in patients with VTE, we lack information on how patients approach HMB treatment decisions.

Objectives: We examined patients' experience with, perceptions of, and preferences for treatment of HMB while on anticoagulation.

Methods: Clinicians and patient advocates developed a web-based survey that was distributed via patient advocacy organizations and social media between May and June 2024, targeting participants 18 years …

Evaluation Of A Novel Tc-24 Recombinant Antigen Elisa For Serologic Detection Of Trypanosoma Cruzi Infection In Dogs: A Pilot Study, Rojelio Mejia, Guilherme G Verocai, Ilana A Mosley, Ashley B Saunders, Bin Zhan, Lindsey Vongthavaravat, Rachel E Busselman, Sarah A Hamer

Faculty, Staff and Students Publications

Chagas disease, caused by the protozoan Trypanosoma cruzi, is a significant challenge for canine health, with limited diagnostic tools. This study assessed the performance of a novel Tc-24 recombinant antigen ELISA compared to three commercial diagnostic tests on 70 serum samples from kennel dogs in Texas. The Tc-24 ELISA demonstrated high sensitivity (87.5%) and specificity (91.2%) compared to Indirect Fluorescent Antibody (IFA) testing, with significant differences in Tc-24 optical density (OD) between positives and negatives (0.607 vs. 0.089, p < 0.001). When compared to Chagas Stat-Pak (SP), Tc-24 ELISA showed sensitivity and specificity values of 82.1% and 86.7%, respectively (p < 0.001). For Chagas Detect Plus (IB), the assay achieved a sensitivity of 80.0% and specificity of 93.1% (p < 0.001). Spearman correlation analysis revealed significant associations between Tc-24 OD and SP (r = 1.0, p = 0.0167), IB (r = 0.9, p = 0.0833) and IFA (r = 0.6273, p = 0.044). Elevated immunochromatographic values in commercial kits (SP or IB) correlated with Tc-24 OD (1.17 vs. 0.039, p < 0.001), achieving 100% sensitivity and 95.8% specificity based on positive results from commercial kits. These findings suggest that Tc-24 ELISA is a reliable and accurate serological tool for diagnosing T. cruzi infection in dogs, with the potential to enhance diagnostic capabilities in veterinary medicine.

Cognitive Performance In Early Neuronal Synuclein Disease With Hyposmia But Without Motor Disability: Association With Dopamine Deficiency And Isolated Rapid Eye Movement Sleep Behavior Disorder, Daniel Weintraub, Anuprita R Nair, Ryan Kurth, Michael C Brumm, Christine Kohnen, Michele K York, Roseanne D Dobkin, Kenneth Marek, Caroline Tanner, Tanya Simuni, Andrew Siderowf, Douglas Galasko, Lana M Chahine, Christopher Coffey, Kalpana Merchant, Kathleen L Poston, Tatiana Foroud, Brit Mollenhauer, Ethan G Brown, Karl Kieburtz, Mark Frasier, Sohini Chowdhury, Roy N Alcalay, Aleksandar Videnovic, Parkinson's Progression Markers Initiative

Faculty, Staff and Students Publications

Objective: To determine the impact of dopamine deficiency and isolated rapid eye movement (REM) sleep behavior disorder (iRBD) on cognitive performance in early neuronal α-synuclein disease (NSD) with hyposmia but without motor disability.

Methods: Using Parkinson's Progression Markers Initiative baseline data, cognitive performance was assessed with a cognitive summary score (CSS) derived from robust healthy control (HC) norms. Performance was examined for participants with hyposmia in early NSD-Integrated Staging System (NSD-ISS), either stage 2A (cerebrospinal fluid α-synuclein seed amplification assay [SAA]+, dopamine transporter scan [DaTscan]-) or 2B (SAA+, DaTscan+).

Results: Participants were stage 2A (n = 101), stage 2B (N …

Microbiome Signatures And Inflammatory Biomarkers In Culture-Negative Neonatal Sepsis, Morcos Hanna, Shixia Huang, Matthew Ross, Anaid Reyes, Dimuthu Perera, Anil Surathu, Sara Javornik Cregeen, Joseph Hagan, Mohan Pammi

Faculty and Staff Publications

Overuse of antibiotics is a concern in 'culture-negative sepsis' but it is unclear whether this is due to infection with viruses, fungi or other microbes that are not easily cultured, or whether it results from inflammatory processes. In a prospective study, we enrolled 50 preterm neonates with culture-positive sepsis (CP), culture-negative sepsis (CN), and asymptomatic preterm controls (CO). The microbiome of stool, skin, and blood, including bacterial, viral and fungal components and serum cytokine profiles were evaluated. The microbiome alpha or beta diversity did not differ between CN and CO groups. A MaAsLin analysis revealed increased relative abundances of specific …

Cas 231n.50: Pharmacology And Addictions, Mary B. Mchugh

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Bioe 370.01: General Ecology, Jedediah F. Brodie

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Fors 241n.00: Dendrology, C. Alina Cansler

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Geo 201.B01: The Rocky Planet, Marc S. Hendrix

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Nrsm 212n.00: Ecology, Physics, And Taxonomy Of Soils, Justin D. Gay

University of Montana Course Syllabi, 2021-2025

No abstract provided.

Ptrm 582.01: Concepts In Wilderness And Protected Area Management, William L. Rice

University of Montana Course Syllabi, 2021-2025

No abstract provided.