Digital Commons Network^™

Facts And Hopes Of Chimeric Antigen Receptor-Redirected Nk T Cells, Amy N Courtney, Xin Zhou, Gengwen Tian, Ying Wang, Leonid S Metelitsa, Gianpietro Dotti

Faculty, Staff and Students Publications

Chimeric antigen receptor (CAR)-engineered invariant NK T cells (CAR-NKT) are a novel cell platform for cancer immunotherapy. Unlike conventional T cells, NKTs are characterized by innate antitumor properties, minimal alloreactivity, and a unique ability to modulate the tumor microenvironment. This article provides a comprehensive overview of preclinical and early clinical studies evaluating CAR-NKTs in both autologous and allogeneic clinical settings. We discuss the contributions of CAR signaling domains, cytokine coexpression, and other functional measures that correlate with CAR-NKT persistence, function, and metabolic fitness. We also discuss the critical role of immunocompetent animal models in elucidating the interactions of CAR-NKTs with …

Low-Dimensional Signatures Of Neuronal Activity Associated With Long-Term Operant Conditioning In Aplysia, Sara Vanaki, Nicholas O Gonzalez, Curtis L Neveu, Yuto Momohara, Behnaam Aazhang, John H Byrne

Faculty, Staff and Student Publications

Operant conditioning is a form of learning in which a behavior is reinforced by reward. Operant conditioning has multiple temporal domains, ranging from short-term, lasting a few minutes, to long-term, persisting for at least 24 h. The extent to which short- and long-term operant conditioning memories rely on shared or separate neural mechanisms is poorly understood. Voltage-sensitive dye (VSD) imaging has been used previously to record the activity of a large number of neurons simultaneously in the buccal ganglion to measure changes in neuronal activity during short-term operant conditioning. We examined neuronal activity using VSD 24 h after operant conditioning …

Computational Study Of Cavitation In An Inducer Pump, Jaylen Leonard

UNLV Theses, Dissertations, Professional Papers, and Capstones

Cavitation is ubiquitous in liquid-handling turbomachines. Cavitation bubbles form when the local pressure drops below a fluid’s vapor pressure, and their subsequent collapse causes implosions that generate shockwaves and microjets. These effects lead to fluid instability and structural damage to the blades and casing in turbomachines. This thesis investigates this cavitation phenomenon in turbomachines using Computational Fluid Dynamics (CFD). The cavitation model built in ANSYS Fluent® is first validated in a venturi tube model and then applied to an inducer pump. The geometry of the pump is derived from the Space Shuttle Main Engine (SSME) Low Pressure Oxidizer Pump (LPOP) …

Gaming: An Untapped Resource For Women In Computer Science Careers?, Claudia Quynh Luu

UNLV Theses, Dissertations, Professional Papers, and Capstones

This research focused on women who identify as gamers (i.e., an individual who regularly plays video games) with the goal of better understanding how being a gamer might help or hinder women’s interest in computer science careers. In particular, I examined the connection between interest in computer science careers, gaming, sexual harassment experiences, coping strategies, and allyship for women in gaming using a mixed-methods approach. The sample (N = 166) included women identifying as previous or current gamers. Participants were recruited from a psychology participant pool and gaming communities on Discord. We conducted a series of analyses to examine whether …

First-Principles Insights Into Binary Oxide Systems Under Extreme Pressure, Daniel Schacher

UNLV Theses, Dissertations, Professional Papers, and Capstones

Pressure is powerful tool by which one can manipulate the properties of materials. In this work we utilize pressure to explore binary oxide systems and characterize their structural and electronic properties. The observation of anomalous structural and electronic behavior in the rutile to CaCl2 phase transition in SnO2 led to the prediction that such behavior is inherent to all oxides experiencing such a phase transition sequence.[1] Here, the ultra-wide band gap semiconductor GeO2 is confirmed to exhibit anomalous behavior during the rutile to CaCl2 phase transition. A phase pure rutile GeO2 sample synthesized under high-pressure, high-temperature, conditions is probed using …

Acute And Chronic Effects Of Neuroinflammation On Hippocampus And Anterior Cingulate Cortex Network Dynamics, Lauren A. Crew

UNLV Theses, Dissertations, Professional Papers, and Capstones

Neuroinflammation has emerged as a critical modulator of cognitive function and neural circuit dynamics, yet the distinct contributions of acute and chronic neuroinflammation to brain function remain poorly defined. This dissertation examines how inflammation independently alters large-scale neural activity and behavior, focusing on interactions between the hippocampus (HPC) and anterior cingulate cortex (ACC), regions essential for memory and executive processes. Using in vivo electrophysiological techniques in a rodent model, this study compares the effects of short-term (acute) and prolonged (chronic) neuroinflammatory states, induced via Poly I:C, on neural oscillations, regional coherence, and spatial coding. Findings reveal that even transient inflammation …

Quantifying Biocrust Evaporation In Arid Lands By Combining Thermal Imaging And Lysimeter Measurements, Rebekah C. Magness

UNLV Theses, Dissertations, Professional Papers, and Capstones

Biological soil crusts (biocrusts) are miniature ecosystems of living organisms incorporated within the soil matrix and established in the topmost millimeters of the soil surface. Biocrust plays a critical role in the hydrologic processes in arid lands, yet the extent to which it increases or decreases evaporation remains unclear. To gain a better understanding of the impact of biocrust on evaporation compared to bare soil, I studied a cyanobacteria-dominated biocrust patch surrounded by bare soil atop a lysimeter in the Mojave Desert near Las Vegas, Nevada. Using high-resolution thermal imaging, and the known latent heat flux from the lysimeter, I …

Geometric And Proximity Based Substrate Targeting By Cullin-Ring Ligases, Jerry Li

UNLV Theses, Dissertations, Professional Papers, and Capstones

Homeostasis, the process of maintaining stable biochemical and biophysical conditions, is a fundamental facet of cellular viability. Biochemical homeostasis is critical to support the biological functions that sustain living organisms. Protein ubiquitylation is one of the primary cellular pathways that regulate protein homeostasis and turnover. Ubiquitylation is initiated through the conjugation of a small globular protein known as ubiquitin, onto a given substrate. There exists a myriad of cellular fates that ubiquitylated proteins may undergo, but the most widely studied pathway is protein degradation, whereby a ubiquitylated protein substrate is shunted to a large macromolecular protease known as the proteasome, …

Ankimedbench: Evaluating Hierarchical Medical Knowledge In Language Model Embeddings, Neel Patel

UNLV Theses, Dissertations, Professional Papers, and Capstones

Despite achieving over 90% accuracy on medical benchmarks, recent studies show physicians cannot effectively leverage language models to improve clinical reasoning. Current benchmarks test isolated factual recall, but clinical practice requires hierarchical navigation through diagnostic categories—starting broad and narrowing systematically from chest pain to cardiovascular pathology to myocardial infarction to specific STEMI types. Existing evaluations cannot measure whether models preserve this taxonomic structure essential for clinical reasoning.

We introduce AnkiMedBench, built from 16,512 medical flashcards used by students preparing for licensing exams. Cards are organized across six hierarchy levels spanning 16 broad medical specialties to 672 specific diseases and conditions. …

Deciphering The Crl Code: Novel Mechanisms Of Canonical And Non-Canonical Ubiquitylation, Nicholas Wayne Purser

UNLV Theses, Dissertations, Professional Papers, and Capstones

Protein degradation is a fundamental process required for maintaining cellular homeostasis in eukaryotic organisms. In humans, distinct cell types exhibit unique proteomic landscapes that are dynamically balanced by the continual synthesis and degradation of proteins. The timely removal of damaged, misfolded, or old proteins is essential to permit new protein synthesis and to ensure proper cellular function.

Central to this regulatory network is ubiquitylation, a highly conserved posttranslational modification system that governs protein stability and function across all eukaryotic cells. Through the covalent attachment of ubiquitin molecules to protein substrates, this system modulates a broad range of biological processes, including …

Quantum Sensing Of Magnetic And Thermal Fields Using Nitrogen-Vacancy Centers On Spoof Surface Plasmon Waveguides, Maxwell Stonham

UNLV Theses, Dissertations, Professional Papers, and Capstones

Nitrogen-vacancy (NV) centers in nanodiamonds are solid-state quantum defects capable of detecting magnetic fields and temperature at the nanoscale with high spatial resolution and optical addressability at room temperature. Their spin-dependent fluorescence enables optically detected magnetic resonance (ODMR), a technique widely used for quantum sensing. However, enhancing optical readout efficiency remains a critical challenge for scalable integration and various methods can be used to improve this.

This thesis explores a hybrid quantum sensing platform that integrates NV-based sensing with spoof surface plasmon waveguides designed to enhance optical excitation and emission efficiency through localized spoof plasmon modes supported by subwavelength corrugated …

Deaf College Students’ Lived Experiences In Pk-16 Education: A Deaf Crit-Informed Qualitative Study, Bridget Catherine Taylor

UNLV Theses, Dissertations, Professional Papers, and Capstones

This qualitative study focused on Deaf, Hard-of-Hearing, and orally Deaf college students, examining Deaf education from the perspective of linguistic and cultural minority students. The study offers theoretical perspectives for researchers, practitioners, and policymakers by examining the lived experiences of students who took part in Deaf education in the United States from PK–16. Deaf Critical Theory (DeafCrit) was the theoretical framework used to analyze the qualitative data.

Enviromental Dna (Edna) Tools For Invasive Mosquitofish (Gambusia Affinis) Surveillance At The Shoshone Pupfish (Cyprinidon Nevadensis Shoshone) Refugium, Yusuf Olamilekan Umar

UNLV Theses, Dissertations, Professional Papers, and Capstones

Spring-fed aquatic ecosystems of the North American Southwest and U.S. Great Basin hosts a plethora of endemic fish species due to the effects of hydrologic fragmentation that resulted from Pleistocene aridification. Threats to endemic fishes worldwide include human intentional or unintentional release of exotic species into their habitats. Early detection of invasive species, while corrective actions are still possible, is critical for management, yet traditional survey methods are often disruptive to sensitive ecosystems, costly, and limited in sensitivity. The recent development of environmental DNA (eDNA) approaches offers powerful alternatives for invasive species monitoring in aquatic systems. In this study, a …

Targeting Kinesin Family Member 20a Sensitizes Stem-Like Triple-Negative Breast Cancer Cells To Standard Chemotherapy, Yayoi Adachi, Weilong Chen, Cheng Zhang, Tao Wang, Nina Gildor, Rachel Shi, Haoyong Fu, Masashi Takeda, Qian Liang, Fangzhou Zhao, Hongyi Liu, Jun Fang, Jin Zhou, Hongwei Yao, Lianxin Hu, Shina Li, Lei Guo, Lin Xu, Ling Xie, Xian Chen, Chengheng Liao, Qing Zhang

Faculty, Staff and Student Publications

Triple-negative breast cancer (TNBC), being both aggressive and highly lethal, poses a major clinical challenge in terms of treatment. Its heterogeneity and lack of hormone receptors or HER2 expression further restrict the availability of targeted therapy. Breast cancer stem cells (BCSCs), known to fuel TNBC malignancy, are now being exploited as a vulnerability for TNBC treatment. Here, we dissected the transcriptome of BCSCs and identified kinesin family member 20A (KIF20A) as a key regulator of BCSC survival and TNBC tumorigenesis. Genetic depletion or pharmacological inhibition of KIF20A impairs BCSC viability and tumor initiation and development in vitro and in vivo. …

Beyond Additive Risk: Longitudinal Associations Between Discrimination And Positive Affect Development Among Adolescents With Single And Multiple Marginalized Identities, Vanni Jefferson V. Arcaina

UNLV Theses, Dissertations, Professional Papers, and Capstones

Adolescents with marginalized identities often face chronic exposure to discrimination, a social stressor linked to a range of negative mental health outcomes. While much of the existing literature focuses on internalizing symptoms, fewer studies have explored how discrimination may disrupt the development of positive affect (POA), which is a core dimension of emotional well-being linked to resilience, identity formation, and long-term health. The present study examined how overall, race/ethnicity-based, and sexual minority (SM)-based discrimination affects longitudinal patterns of POA among adolescents with single or multiple marginalized identities. Drawing on four waves of data from the Adolescent Brain Cognitive Development Study, …

Automated Spectrum Analysis And Radionuclide Identification For Robot-Based Gamma Probes, Kennedy Ochieng Mengoh Alila

UNLV Theses, Dissertations, Professional Papers, and Capstones

Gamma spectroscopy is a technique used for the detection and identification of nuclear materials. Gamma-ray emission by radio nuclides is detected using scintillation detectors or semiconductor detectors such as NaI and CZT. Robot-based gamma probes enable radiation measurements in large areas and hard-to-reach cluttered hazardous environments, which significantly reduces radiation risks to personnel. The robotic probes require automated on-board gamma spectrum analysis and capabilities with platforms (unmanned aerial vehicles and unmanned ground vehicle systems) which use Robot Operating System (ROS). The aim of this thesis was to develop a method of an automated spectral analysis and isotope identification for robot-based …

Functional And Microbial Consequences Of Experimental Evolution Under Starvation In Drosophila Melanogaster, Kristal Marie Aguilar-Conger

UNLV Theses, Dissertations, Professional Papers, and Capstones

Previous studies in the Gibbs lab have shown that starvation resistant Drosophila melanogaster flies have increased lipid content, lower metabolic rates, and poor physiological health. Historically, many evolutionary studies end at the phenotypic level, leaving the genomic basis largely unresolved. Using Evolve and Resequence (E&R) study, we can map genome-wide allele-frequency trajectories under selection, linking phenotypic shifts to their genomic underpinnings. This study utilized the E&R approach to identify enriched biological processes and pathways underlying starvation adaptation in Drosophila melanogaster. Five replicate restart populations (RS A–E) selected for starvation resistance, along with a control (RC), were resequenced at Generations 1, …

Protocol For Isolation Of Lipid Droplets And Mitochondria From Acute Myeloid Leukemia Cells For Lipidomic Analysis, Ran Zhao, Liang Zhang, Shuxin Dong, Kazuharu Kamachi, Saurabh Kumar Gupta, Hiroki Akiyama, Jalen Nguyen, G Lavender Hackman, Michael Andreeff, Alessia Lodi, Jo Ishizawa

Faculty, Staff and Student Publications

Lipid droplets (LDs) are dynamic organelles critical for lipid and energy homeostasis. LD dysregulation is implicated in various diseases, including acute myeloid leukemia (AML), which exhibits aberrant lipid metabolism and chemoresistance. Here, we describe an effective protocol for the co-isolation of LDs and mitochondria from lipid-deficient cell types, e.g., AML cells. We detail procedures for the isolation, quality verification, and results from mass spectrometry-based lipidomic analysis. This protocol enables the functional study of organellar lipidomic regulation in AML.

Elucidating The Role Of Pbaf Chromatin Remodeling Subunit Arid2 In B Cell Development, Rachel Paolini

Arts & Sciences Graduate Student Theses and Dissertations

B cells are key components of the adaptive immune system that mediate humoral immunity through the production of antigen-specific antibodies. The generation of functional B cells involves multiple stages of differentiation across distinct anatomical niches, requiring precise regulation of microenvironmental signals and intrinsic transcriptional networks. SWI/SNF chromatin remodeling complexes have emerged as key regulators of B cell lineage commitment by modulating the accessibility of transcriptional programs required for differentiation and maturation. However, the specific roles of individual SWI/SNF subunits and distinct complexes within early B cell differentiation remain poorly understood. In this thesis, I investigated the function of Arid2, an …

Meta-Analysis Of The Spatial Distribution And Composition Of Plastic Macro-Debris In Indonesia, Max R. Kelly, Muhammad Reza Cordova, Susan Jobling, Richard C. Thompson

School of Biological and Marine Sciences

Plastic pollution is recognised as a global environmental problem with Indonesia identified as a major contributing source. However, a comprehensive understanding of pollution patterns remains hindered by fragmented data and methodological inconsistencies across studies. This study synthesises data from 44 peer-reviewed publications, spanning 68 field locations and 328 individual study sites across 19 provinces, to map the spatial distribution and composition of plastic macro-debris in Indonesia. We identified plastic bags, plastic food packaging, and plastic bottles as the three most prevalent item types, occurring as top three ranked items in 65 %, 63 %, and 40 % of field locations, …

Methylation-Based Signature To Distinguish Indolent And Aggressive Prostate Cancer, Muheng Liao, Jace Webster, Amy Ly, Emily Rozycki, Christopher A Maher

2020-Current year OA Pubs

Prostate cancer management faces significant challenges in distinguishing indolent from aggressive disease, particularly since most patients are intermediate-risk and therefore hinders the ability to recommend standardized treatment recommendations. Moreover, current prognostic tools including Gleason scoring and tumor staging demonstrate limited accuracy for predicting disease progression and tumor recurrence. DNA methylation serves as a stable epigenetic modification that directly regulates gene expression, making it an ideal biomarker for cancer prognosis. Therefore, this study leveraged whole-genome enzymatic methylation sequencing on 120 patients to develop a novel prognostic signature for aggressive prostate cancer progression. We analyzed 20,849 differentially methylated regions (DMRs) and employed …

Loss Of Mfap5 And Its Effect On Skin Homeostasis And Wound Healing, Chen Han, Heidi Yuan, Timothy J Koh, Lin Chen, Robert P Mecham, Luisa A Dipietro

2020-Current year OA Pubs

MFAP5, also known as microfibrillar-associated glycoprotein-2 (MAGP2), may influence parameters of skin wound healing related to scar formation. To further elucidate its role in skin wound healing, we assessed skin wound repair in Mfap5

Role Of Recreational Fisher Information In Fisheries Management, Rebecca M. Krogman, Stephen M. Taylor, Aaron J. Adams, Ross E. Boucek, Steven M. Lombardo, Chelsey A. Crandall, Susan F. Steffen, Alejandro Acosta, Eduardo Bone Moron, Sepp Haukebo, Kieran Hyder, Zachary M. Radford, Warren M. Potts

Books & book chapters

Fisheries managers work with the three components of fisheries—biota, environment, and people - to achieve recreational, stewardship, and socioeconomic goals. Although manipulation of biota and the aquatic environment remains popular (e.g. harvest regulation, protecting or enhancing habitats, and guarding water quality), the human dimension of fisheries must also be considered throughout the fisheries management process. Managers can and should engage fishers in a myriad of ways, from identification of problems to guidance on alternatives, and from measurement of change to evaluation of success. In this chapter, we review common fisheries management goals and objectives; detail information needs stemming from those …

How Native Landscaping And Irrigation Can Conserve Water In Mediterranean Climates, Samuel Carlson

Sustainable Supply Chain Management

This paper explores how using native plants and proper irrigation can lead to sustainable water management in Mediterranean climates, and reinterprets water scarcity as a supply chain issue. Touching on growing populations, climate change, and water pollution, it examines international case studies from Chile, Egypt, and Spain to compare domestic examples at multiple California universities.

Powering Up Clinical Practice: Mastering Clinical Literature Search Techniques In Embase For Pediatric Hospital Medicine Fellows, Evelyn Wang

HSLIC Faculty and Staff Instructional Resources

This didactic EMBASE session for Pediatric Hospital Medicine (PHM) fellows demonstrates how to translate pediatric clinical questions into EMBASE search strategies. It use of keywords and Emtree, proximity and Boolean operators, PV Wizard, device and drug searches, and workflows for organizing results to support clinical decision‑making

A Novel Of Bounded Zeghdoudi Distribution: Estimations, Simulation And Applications, Mohammed Elgarhy, Hanene Hamdani, Laxmi Prasad Sapkota, Ahmed M. Gemeay

Mesopotamian Journal of CyberSecurity

In this article, we introduce a new unit Zeghoudi distribution, which is called the bounded Zeghoudi distribution (BZD), an innovative modification of the Zeghoudi distribution (ZD). This new suggested distribution retains the original ZD’s simplicity while improving modeling flexibility and accuracy for data constrained to the unit interval. The BZD displays numerous significant characteristics, including decreased, left-skewed, right-skewed, and unimodal probability density functions, but the hazard rate function can be J-shaped, U-shaped, and bathtub-shaped. Some important statistical features of the BZD, such as moments, mean, variance, moment generating function, lower and upper incomplete moments, mean residual life, mean inactivity time, …

Content Aware Intra And Inter Prediction Using Texture And Motion Analysis, Yashaswi Karthik Reddy Danda

Theses

Modern video codecs rely on highly optimized block based intra and inter prediction frameworks,  in which block partitioning is determined through exhaustive rate distortion optimization (RDO)  searches. These exhaustive searches evaluate many possible block configurations to identify the  partitioning that minimizes overall rate distortion cost, and they have proven extremely effective  across diverse visual content. This thesis explores a complementary perspective by investigating  whether block partitioning can be guided more directly by content characteristics specifically,  texture complexity for intra prediction and motion complexity or motion density for inter  prediction.  For intra prediction, we introduce a texture adaptive approach that utilizes …

The Effect Of Phosphate Fertilizer And Organic Matter On Phosphorus Status, Ph, And Cabbage Yield In Andisols, Mahfud Arifin, Emma Trinurani Sofyan, Anni Yuniarti, Rina Devnita, Sastrika Anindita

Jurnal Kultivasi

Andisols are characterized by high phosphorus (P) retention, which often limits P availability for crops. This study aimed to analyze the independent and interactive effects of P fertilizer and organic matter (i.e. manure and rice straw compost) on P retention, total P, available P, P uptake, soil pH, and cabbage yield. This research was conducted in pot experiments in plastic house, applying P fertilizer (0, 90, 180, 270 kg ha-1) and organic matter (20 kg ha-1 of chicken, sheep, cow manures and rice straw compost) on Andisols planted with cabbage. The experiment used a factorial randomized design. The results showed …

Biophysical Simulation Enables Segmentation And Nervous System Atlas Mapping For Image First Spatial Omics, Lina Mohammed Ali, Aldrin Kay Yuen Yim, Emanuel Gerbi, Thien Nguyen, Nicholas Tu, Faith Ikede, Remi Sampaleanu, Diana Grigore, Jason Waligorski, Colin Kremitzki, Liya Yuan, Wendy Dong, Robi Mitra, Jeffrey Milbrandt, William Buchser

2020-Current year OA Pubs

Spatial omics (SO) produces high-definition mapping of subcellular molecules within tissue samples. Mapping transcripts to anatomical regions requires segmentation, but this remains challenging in tissue cross-sections with tubular structures like axons in peripheral nerve or spinal cord. Neural networks could address misidentification but are hindered by the need for extensive human annotations. We present SiDoLa-NS (Simulate, Don't Label-Nervous System), an image-driven (top-down) approach to SO analysis in the nervous system. We utilize biophysical properties of tissue architectures to design synthetic images of tissue samples, eliminating reliance on manual annotation and enabling scalable training data generation. With synthetic samples, we trained …

Effects Of Supplemental Led Lighting And Biofertilizer On Yield And Fruit Quality Of Japanese Strawberry Cultivars In Indonesia, Reginawanti Hindersah, Febby Fitriyani, Kenji Endo, Masakazu Nakayama, Syariful Mubarok, Gita Bina Nugraha

Jurnal Kultivasi

Growing strawberries in soilless substrates in the greenhouse relies on fertilizers and supplemental light to boost yield and fruit quality. The research evaluated the properties of the biofertilizer and its effectiveness on the yield of Japanese strawberry cultivars Nanatsuboshi and KS-75 under controlled greenhouse conditions, with and without light-emitting diodes (LED) as supplemental light. The cell viability and plant-growth-promoting properties of a liquid consortium biofertilizer consisting of nitrogen-fixing bacteria and phosphate-solubilizing microbes, were characterized. Laboratory analysis confirmed the biofertilizer's composition, and a greenhouse experiment using a completely randomized design tested treatments of biofertilizer, LED, and their combination. The biofertilizer contained …