Digital Commons Network^™

Construction And Optimization Of An Efficient Tyrosol Biosynthesis System In Escherichia Coli, Yizhe Xiong, Shougang Wang, Zhiming Rao, Meijuan Xu

Journal of Food Science and Biotechnology

[Objective ] An efficient biosynthetic system was constructed in Escherichia coli to achieve high-yield production of tyramine and its key derivative tyrosol.[Method ] Through the analysis of protein expression,enzymatic properties,and whole-cell catalysis results,tyrosine decarboxylase (TDC ) derived from Enterococcus faecalis was selected as the key enzyme for tyramine synthesis.Subsequently,tyramine oxidase (TYO ) was introduced to construct the TDC-TYO pathway for tyrosol production.Meanwhile,the LAAD-PDC pathway for tyrosol synthesis was established.A dual-enzyme co-expression system was established,and the effects of tandem enzyme order,production pathways,and key enzyme sources on tyrosol yield were analyzed.The recombinant strain B 1,carrying the plasmid pET 28a-EfTDC-TYO,was obtained through whole-cell …

Promoting The Synthesis Of Salicylic Acid In Escherichia Coli Based On Metabolic Engineering And Multi-Enzyme Cascade Strategy, Kai Wang, Xuewei Pan, Taowei Yang, Zhiming Rao

Journal of Food Science and Biotechnology

[Objective ] The production of the aromatic compound salicylic acid (SA) is limited by its inhibitory effects on enzymes related to cellular energy metabolism.This study aims to develop a strategy for avoiding the impact of SA on enzymes related to cellular energy metabolism and increase the yield of SA.[Method ] A highly efficient Escherichia coli strain was designed to improve SA production by optimizing metabolic pathways and enzyme systems.[Result ] The genome-scale metabolic model GEMS 1515 was employed to predict the key bottle necks in the phosphoenolpyruvic acid (PEP) pathway and SA biosynthesis pathways.The SA yield was successfully increased from …

Fermentation Technology Of L-Lysine Production By Immobilized Corynebacterium Glutamicum, Qingguo Liu, Hanjie Ying, Wenjun Sun

Journal of Food Science and Biotechnology

[Objective ] This study aimed to investigate the feasibility of the fermentation process for L-lysine production by immobilized Corynebacterium glutamicum.[Method ] Firstly,the effects of different volume fractions of seed medium on the fermentation stability of immobilized Corynebacterium glutamicum were examined.Subsequently,the impacts of immobilization carriers with different specifications on the fermentation performance were analyzed.Finally,a comparative analysis was conducted on the differences between the fermentation processes with free and immobilized Corynebacterium glutamicum.[Result ] The addition of 5% volume fraction of seed medium adequately sustained the growth and metabolic activities of Corynebacterium glutamicum.Higher volume fractions diverted carbon sources towards bacterial growth,thereby limiting product …

Reduction Of Methanol Content By Protoplast Fusion In Fruit Wine Yeast, Huixiang Zhang, Nan Lin, Jiaru Wu, Xin Cheng, Weiwu Liang, Shijun Yang

Journal of Food Science and Biotechnology

[Objective ] This study aims to breed a fruit wine yeast strain with low methanol production by protoplast fusion.[Method ] With yeast strains LAB and XR as parents,protoplast fusion was employed to breed the yeast strains for fruit wine fermentation with a low mass concentration methanol and a high volume fraction ethanol.The process of yeast protoplast preparation was optimized by single factor and orthogonal experiments with the formation rate,regeneration rate,and preparation rate of protoplasts as indicators.The parental protoplasts were inactivated by ultraviolet radiation and heating,and 35 g/dL PEG- 6000 was used for chemical fusion after inactivation.[Result ] The optimum conditions …

Effects Of Metschnikowia Pulcherrima And 24-Epibrassinolide On Postharvest Preservation Of Grapes, Jiayan Qu, Huiping Niu, Bowen Li, Xiao Wang, Lujun Wang, Zhumei Xi, Xuefei Wang

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effects of different treatments on the postharvest preservation of grapes.[Method ] The impacts of 0.4 mg/L 24-epibrassinolide (EBR ),1×109 CFU/mL Metschnikowia pulcherrima,and their combination (YBR ) on postharvest storage of ‘Shine Muscat ’ grapes were evaluated.[Result ] Compared with the control (CK) group,EBR,Metschnikowia pulcherrima,and YBR treatments effectively reduced the water loss rate,maintained the firmness,and delayed the declines in protopectin and stem chlorophyll content of grape.In the later storage stage,YBR treatment delayed the decrease in flavonoid mass fraction,which was 12.95% higher than that of CK group.At the same time,YBR treatment reduced the mass …

Efficient Catalytic Synthesis Of 5-Methylpyrazine- 2-Carboxylic Acid By Recombinant Escherichia Coli, Luxi Gan, Heng Gong

Journal of Food Science and Biotechnology

[Objective ] This study aimed to improve the yield and conversion rate of 5-methylpyrazine- 2-carboxylic acid (MPCA ) through biocatalysis.[Method ] Escherichia coli (E.coli）was used as the model organism to construct an MPCA synthesis system,and the whole-cell catalytic reaction conditions of recombinant E.coli were optimized.In addition,the parental host strain was subjected to ultraviolet mutagenesis for the screening of host strains tolerant to high mass concentrations of 2,5-dimethylpyrazine (DMP ).[Result ] The recombinant mutagenized strain could completely convert 10 g/L DMP to MPCA within 12 h in a 10 mL reaction system,achieving a yield of 12.9 g/L and a corresponding molar …

Goat Milk Formula Powder Ameliorates Lipopolysaccharide-Induced Intestinal Inflammation, Yi Shan, Xiaoling Liu, Shumei Wang

Journal of Food Science and Biotechnology

[Objective ] This study aimed to investigate the effects of goat milk formula powder on lipopolysaccharide (LPS)-induced intestinal inflammation and barrier impairment in mice.[Method ] Histopathological observation,real-time quantitative PCR,and enzyme-linked immunosorbent assay were used to assess changes in intestinal inflammation levels and barrier function-related indicators in mice following intervention with goat milk formula powder.Additionally,the composition of the gut microbiota was analyzed to elucidate its potential mechanisms of action.[Result ] Goat milk formula powder alleviated intestinal histopathology and increased the number of goblet cells.Compared with the model group,the goat milk formula powder intervention reduced the mass fractions of pro-inflammatory cytokines TNF- …

Design , Expression , And Stability Evaluation Of A Superoxide Dismutase-Catalase Fusion Protein, Zhuo Jiang, Weina Lu, Qi Lin, Zhan Luo, Zhengbin Lyu, Zuoming Nie

Journal of Food Science and Biotechnology

[Objective ] This study aims to design a fusion protein with high activities and high stability of superoxide dismutase (SOD ) and catalase (CAT ).[Method ] Using AcSOD 11 and FeCAT proteins as the research subjects,three fusion proteins of SOD and CAT were designed:AcSOD 11-(GGGGS )*3-FeCAT,FeCAT-(GGGGS )*3-AcSOD 11,and FeCAT- (EAAAK )*3-AcSOD 11,which were named AcSOD 11-G3-FeCAT,FeCAT-G3-AcSOD 11,and FeCAT-E 3-AcSOD 11,respectively.Soluble expression of these three fusion proteins was achieved in Escherichia coli BL21(DE3).The thermal stability and pH stability of the three fusion proteins were then assessed.[Result ] The three fusion proteins showed no significant difference in the specific activity of SOD,while …

Design And Optimization Of A Geothermal Thermoelectric Generator System (Gteg) In Saudi Arabia, Hassan Fagehi

Journal of King Abdulaziz University: Engineering Sciences

The demand for renewable energy is growing rapidly, driven by climate change and the need for sustainable solutions. This study focuses on designing and optimizing a geothermal thermoelectric generator (GTEG) system in Saudi Arabia, leveraging the country's geothermal potential. The optimization process investigated key parameters such as substrate materials, thermoelectric leg length, and dimensionless thermal and electrical conductance. The results reveal that using AlN as a substrate material, combined with a reduced leg length of 0.1 mm, significantly enhances power output, achieving 8.5 W per module. Dimensionless analysis further identified optimal dimensionless thermal conductance (N_k = 0.291) and dimensionless …

Cv: Maria Josefina Natividad (Medical Assistant, Pharmacy Technican), Maria Josefina Natividad

Health Sciences Department Curricula Vitae

No abstract provided.

Contributors - The Crown, Vol. 2, The Crown

The Crown: Syracuse Honors Research Journal

No abstract provided.

Windkessel Parameter Estimation For Cardiovascular Feature Identification In Photoplethysmography Data, Calvin Boyce

Undergraduate Honors Theses

Features of cardiovascular health such as blood pressure, cardiac output, arterial compliance, and blood viscosity are important in evaluating heart health and disease risk, but are currently largely measured invasively and therefore only in critical settings. Here we explore the potential for Windkessel models to extract cardiovascular features from photoplethysmography (PPG) data in an attempt to evaluate heart health non-invasively. We show the capacity of a constrained Newton's method optimization scheme to estimate parameters and explore the tractability of the resulting parameters. While Newton's method is able to produce parameters that closely approximate PPG data, we found that using a …

Listening To Life In A Noisy World: The Pitfalls And Promise Of Urban Acoustic Ecology, Leah O'Barr

Undergraduate Honors Theses

This thesis examines the application of soundscape ecology principles to urban settings. Soundscape ecology is the study of the entire sonic makeup of a community, composed of biophony (biological sound), geophony (environmental sound), and anthrophony (human-generated sound). By examining the changes in and qualities of the soundscape, researchers can gain information about the biological makeup and ecological quality of an area, and the temporal and spatial distribution of resident species. Recent technological advances in recording equipment and machine learning methods are making soundscape ecology an increasingly relevant and practical tool.

Applying soundscape ecology methods to urban ecosystems introduces unique challenges, …

Editorial Staff - The Crown, Vol. 2, The Crown

The Crown: Syracuse Honors Research Journal

No abstract provided.

The Sociology Of Motherhood In The Workplace, Katharine Batista

The Crown: Syracuse Honors Research Journal

Key themes in this paper include delayed childbearing, uncertain career trajectory, role conflict, and identity blending. These topics highlight the idea that childbearing while possessing a position of power in the workplace is uncommon, which can deter mothers from returning to work after maternity leave. Role conflict is the tension between competing demands of being a mother and a professional. This develops the concept that due to this conflict, women are often expected to adopt a motherly role in the workplace. This extremist ideology surrounding the roles women possess in the workplace, leaves them feeling as though their jobs expect …

Mindfulness And Neurodevelopment In Schools: How Mindfulness-Based Interventions Support Self-Regulation And Learning In Youth, Anna Mirer

The Crown: Syracuse Honors Research Journal

Neurodevelopmental difficulties in childhood and adolescence, including challenges with attention, executive function, and emotional regulation, are increasingly documented across diverse educational settings. These chal-lenges are frequently linked to atypical maturation or disrupted connectivity within large-scale brain networks. The prefrontal cortex (PFC), anterior cingulate cortex (ACC), amygdala, and insula contribute to cognitive con-trol, salience detection, and emotion regulation. Dysregulation in these systems has been implicated in stress sen-sitivity, emotional dysregulation, and reduced executive functioning. Mindfulness-based interventions (MBIs) are increasingly implemented in educational contexts to support the development of self regulatory capacities. MBIs emphasize the practice of nonjudgmental awareness and attentional focus, …

A Spatial Understanding Of Calcium Carbonate Saturation Along The Eastern Coast Of Aotearoa New Zealand: Pacific Oysters Case Study, Anna Isabel Kaufman, Angelina Arcidi

The Crown: Syracuse Honors Research Journal

As ocean acidification continues to become a pressing issue as a result of climate change and increased carbon uptake in the ocean, many species, including the Pacific Oyster, are at risk. This may be due to a decrease in pH levels and, therefore, a decreased calcite saturation state, which in turn hinders their ability to grow and maintain their calcium carbonate shell. The aquaculture in Aotearoa, the Māori word for New Zealand, has become an important socio-economic component for both Māori and Pākehā Aotearoa New Zealand (ANZ) citizens. The following research geographically focuses on the eastern coast of ANZ around …

Shedding Light On Seasonal Depression: Practical Strategies For Pediatric Care, Mike Smith, Sara R. Gould

Pediatrics in Practice: A CME Podcast

As daylight fades and winter approaches, pediatricians often see an uptick in mood-related concerns among children and adolescents. In this episode, we explore how to recognize the signs of seasonal depression, differentiate it from other mood disorders, and apply evidence-based interventions. Our expert guest shares practical screening tools, treatment options—including light therapy and behavioral strategies—and tips for partnering with families and schools to support mental health during the darker months. Whether you’re in primary care or a specialty clinic, this conversation offers actionable insights to help you guide patients through seasonal challenges.

Click here to stream the podcast episode.

When Prayers Aren’T Enough: The Need For Mission Formation And Professional Development For Lay Faculty At Catholic Colleges And Universities, Lucy A. Pesce

Seton Hall University Dissertations and Theses (ETDs)

Current literature suggests that administration and faculty must make a more intentional effort within Catholic higher education to articulate the mission of these religiously-affiliated institutions in response to two current issues: dwindling numbers of clergy and vowed religious faculty and administrators, and fewer students nationwide who self-identify with any form of organized religion. However, few studies have examined the experience of lay faculty at Catholic institutions who are entrusted with advancing the mission, regardless of their religious affiliation, and their professional development needs.

Using the Association of Catholic Colleges and Universities’ Catholic Identity and Mission Assessment (CIMA) as a framework …

The Implementation Of Tact And Bidirectional Intraverbal Training In Teaching A Foreign Language: A Systematic Replication, Karen Parra Caballero

Seton Hall University Dissertations and Theses (ETDs)

The current study replicates and extends Yamaguchi and Matsuda (2024) to evaluate the effects of foreign tact and bidirectional intraverbal training on teaching foreign-language vocabulary to adults. An adapted alternating-treatments design was used to evaluate the effects of learning vocabulary of a foreign language through tact teaching (tacting a picture in French), foreign-native intraverbal teaching (vocalizing a French word in the presence of the Spanish word), and native-foreign intraverbal teaching (vocalizing a French word in the presence of the Spanish word). Each teaching condition had an assigned stimulus set for teaching, and an additional stimulus set was included as a …

Development Of An Evidence-Based Nutrition Protocol For Professional Pickleball Athletes, Laura Morales

Department of Nutrition Student Projects

Professional pickleball has expanded rapidly, yet sport-specific nutrition guidance remains absent. Current information available to athletes comes largely from non-professional or industry sources and lacks an evidence-based foundation. This project aims to address that gap by developing a nutrition protocol tailored specifically for professional pickleball players. The approach synthesizes data from pickleball workload studies, racket-sport physiology, and established sports nutrition consensus statements. Key focus areas include fueling strategies, protein distribution for recovery, hydration and sodium planning, heat acclimation and cooling practices, travel and jet-lag management, altitude considerations, and evidence-based supplementation. By integrating findings from racket-sport analogs and intermittent high-intensity training …

Extracellular Matrix-Mycaf Signatures Correlate With Resistance To Neoadjuvant Apd-L1 Immune Checkpoint Inhibition With Durvalumab + Metformin In Hpv+ Hnscc, Pablo Llerena, Hani Samarah, Kathryn Nunes, Zhao Lin, Kelly Bridgham, Sruti Tekumalla, Amiti Jain, Larry A. Harshyne, Sanket Kumar Shukla, Ioannis Vathiotis, Madalina Tuluc, Stacey Gargano, John R. Eisenbrey, Scott W Keith, David Cognetti, Voichita Bar-Ad, Adam J. Luginbuhl, Rita S. Axelrod, Rajanikanth Vadigepalli, Hushan Yang, Diana Whitaker-Menezes, Megan Roche, My G. Mahoney, Athanassios Argiris, Charalambos C. Solomides, Jennifer Johnson, Md, Mouadh Barbirou, Amanda Miller, Andrew P. South, Ramkrishna Mitra, Alban J Linnenbach, Ubaldo E. Martinez-Outshoorn, Joseph Curry

Department of Otolaryngology - Head and Neck Surgery Faculty Papers

PURPOSE: Immune checkpoint inhibitors (ICI) have demonstrated clinical benefit in head and neck squamous cell carcinoma (HNSCC); however, single-agent efficacy is limited, leaving significant unmet needs. Metformin may synergize with ICIs, offering promise to improve response rates. We leveraged multiomic data from a randomized, presurgical neoadjuvant trial (NCT03618654) evaluating a single infusion of the anti-PD-L1 ICI durvalumab with or without daily, standard dose metformin in previously untreated, nondiabetic patients with HNSCC to understand predictors of response and the effect of combination therapy.

PATIENTS AND METHODS: Clinical, pathologic, and correlative data were analyzed to investigate response and resistance mechanisms. We present …

Migratory Songbirds As Potential Ectozoochorous Protist Dispersal Vectors, Silas E. Fischer, Joy J. Jackson, Elise C. Hoffman, Henry M. Streby, Trisha L. Spanbauer

Earth and Environmental Sciences Faculty Publications

Protist biogeography, speciation, and systematics continue to generate debate and inquiry because protist distributions and dispersal remain poorly resolved. Identifying potential vectors and basibionts for epibiont protists would contribute to our limited understanding of their ecology. Migratory animals seasonally link disparate landscapes, incidentally transporting other organisms in the process. Waterbirds are known microbe dispersers, but evidence for other avian groups is limited-such as taxa that often migrate longer distances. We asked whether terrestrial songbirds (Passeriformes) host diatoms (Bacillariophyta) by sampling tail plumage of four thrush species (Hermit Thrush Catharus guttatus, Swainson's Thrush C. ustulatus, Wood Thrush Hylocichla mustelina, American Robin …

Pronatalism In The United States: How Political Ideals Surrounding Family And Reproductive Health Care Contribute To Neglect In The Foster Care System, Carson Sebree

Undergraduate Honors Theses

Within the past decade, conservative reproductive politics in the United States have experienced an ideological shift from pro-life toward pronatalism. This has been intensified through the rollback of reproductive rights and the rise of Christian nationalism. However, there has been no commensurate additional funding for the foster care system. This project asks the interrelated questions of: “how is pronatalism linked to neglect within the foster care system?; and, how do theories of democratic care highlight the necessity to rethink support for the foster care system and create policies that will address its shortcomings, given the rise of pronatalist aw and …

Fabrication Of Three-Dimensional Seaweed-Based Scaffolds And Evaluation Of Cell Attachment And Viability For Cultured Meat Applications, Srishti Tandon

Honors Scholar Theses

Global demand for animal protein is increasing rapidly, underscoring the need for sustainable meat production. Cultured meat is a promising alternative to traditional meat production techniques, but it requires optimization before it can be implemented on a large scale. Scaffolds are an important component for making cultured meat, as they provide structural support for cell attachment and mimic the texture of meat. Scaffolds need to be biocompatible, mechanically supportive, and biochemically active to promote proper cell adhesion, proliferation, and differentiation. This project investigated brown seaweed extract (SWE) hydrogels as a scaffold for cultivated meat applications and compared their mechanical and …

Rab32c Mediates Osmoregulation And Calcium Homeoastasis In Dictyostelium Discoideum, Ashley Esparza

Theses and Dissertations

Vesicular trafficking is a fundamental process essential to cell viability and dictates roles in both cellular and tissue function in multicellular organisms. To further understand various vesicular trafficking processes, our lab focuses on the moderators of these processes, Rab GTPases, using the model organism Dictyostelium discoideum. This dissertation focuses explicitly on the uncharacterized protein Rab32C, a homolog of human Rab32. While several Rab family members have been well defined, the functionality of Rab32C remains poorly understood. This project investigated both the subcellular localization and functional role of Rab32C with biochemical, imaging, and molecular assays. Results from this study provide evidence …

Plant Preservation: The Contribution Of Bacteriophage And Transposons To Pathogenic Xanthomonas Evolution, And The Demographic And Reproductive Analysis Of Castilleja Parvula, Ciara A. Green

Theses and Dissertations

Plant preservation, whether through disease research or endemic extinction analyses, plays an important role in the relationship between humans and our surrounding environment. In these analyses, both the role of mobile elements in the evolution of pathogenic Xanthomonas sp. bacteria, and the demographic and reproductive analysis of Castilleja parvula, an imperiled alpine plant, are evaluated. Xanthomonas species are one of the top agricultural threats, and are known to evolve rapidly. Until now, the contributions of mobile elements and particularly bacteriophage have not been thoroughly evaluated. The contribution of these agricultural diseases to food insecurity and the devastation of crops that …

Understanding The Relationship Between Mental Health And Criminal Justice Involvement: Pathways, Disparities, And Improving System Responses, Haley Bly

Theses

This capstone project examines the complex relationship between mental health and involvement in the criminal justice system, emphasizing how pathways, external conditions, structural inequities, and system responses interact to shape long-term outcomes for persons with mental illness (PwMI). While mental illness alone is not a predictor of criminal behavior, the intersection between psychological disorders, social strain, stigma, and systemic failures significantly heightens the risk of criminal justice involvement. Combining four working papers, this capstone examines these relationships across individual, environmental, institutional, and community levels, ultimately arguing that treatment and rehabilitative-centered reforms are essential for improving the experiences of individuals with …

Elucidating The Role Of Pbaf Chromatin Remodeling Subunit Arid2 In B Cell Development, Rachel Paolini

Arts & Sciences Graduate Student Theses and Dissertations

B cells are key components of the adaptive immune system that mediate humoral immunity through the production of antigen-specific antibodies. The generation of functional B cells involves multiple stages of differentiation across distinct anatomical niches, requiring precise regulation of microenvironmental signals and intrinsic transcriptional networks. SWI/SNF chromatin remodeling complexes have emerged as key regulators of B cell lineage commitment by modulating the accessibility of transcriptional programs required for differentiation and maturation. However, the specific roles of individual SWI/SNF subunits and distinct complexes within early B cell differentiation remain poorly understood. In this thesis, I investigated the function of Arid2, an …

Trauma Beyond Time: Temporal Constructs In Holocaust Testimonies, Sarah Seiselmyer-Snyder

Purdue University Press Books

Trauma Beyond Time: Temporal Constructs in Holocaust Testimonies challenges our understanding of what it means to be a Holocaust survivor, arguing that the term “post-Holocaust” fundamentally misrepresents survivors’ experiences. Through careful analysis of Holocaust literature and testimony, this book reveals how trauma persists across generations, defying conventional historical timelines. For those who perished, there can be no “after” to the Holocaust—their stories were violently ended. Yet for survivors, the Holocaust didn’t simply conclude in 1945. Their experiences demonstrate how trauma continues to shape lives decades later, making “post-Holocaust” a misleading concept that fails to capture their ongoing reality. Using the …