Digital Commons Network^™

Microrna-Mediated Regulation Of The Parkinson's Disease-Associated Gene, Lrrk2, Peyton B. Hanser, Pavithra Madamarandawala, Wendy Olivas

Undergraduate Research Symposium

Parkinson’s Disease (PD) is an age-related neurodegenerative disease. LRRK2 is one of the main PD-associated genes, as both familial mutations and its abnormal overexpression are associated with PD progression. However, underlying molecular mechanisms leading to LRRK2 abnormal overexpression are not well understood. MicroRNAs (miRNAs) are a group of small non-coding RNAs that regulate target messenger RNAs (mRNAs) by binding to a specific sequence in their 3’ UTRs (untranslated regions at the ends of mRNAs) and blocking translation of the mRNA. Previous studies have identified miR-205 as one regulator of LRRK2. However, the LRRK2 3’ UTR has many other computationally-predicted miRNA …

Phenotypic Variations Between The European Paper Wasps, Senna V. Horak, Thomas Hagan, Sara Miller

Undergraduate Research Symposium

The European paper wasp (Polistes Dominula) is a widespread invasive Hymenoptera species in the US, commonly known for their black and yellow markings. Phenotypic variations, primarily in color patterns and size, play a significant role in understanding their interactions among other wasps and effects of environmental factors on the species. In this study, we examined phenotypic variation within and between populations by measuring head width, intertegular distance, and femur and tibia length.

Habitat Disturbance And Biodiversity In Glade Habitats, Maddie Smith, Becky Hansis-O'Neill, Aimee Dunlap Phd

Undergraduate Research Symposium

The Ozark glades are home to a variety of reptiles, amphibians, rodents, and invertebrates such as tarantulas and scorpions. Glade inhabitants face challenges like habitat fragmentation and degradation, but they could be facing even bigger challenges as outdoor recreation becomes more popular. People looking for wildlife to photograph or collect often look under rocks without leaving them how they were found. This study focused on monitoring rock disturbance at two protected glades- Shaw Nature Preserve and a more accessible undisclosed glade in Jefferson County. Disturbing the glade habitat to look for wildlife is prohibited at both sites. Twenty rocks at …

Face Value: A Computational Approach To Subjective Impressions Of Faces, Kevin Kpankou

Undergraduate Research Symposium

Various computational models of first impressions have been developed to uncover the mechanisms driving these judgments. However, the implicit notion of a singular ``human'' often overlooks meaningful individual differences in beliefs, attitudes, and associations, as well as culturally grounded group-level constructs. In this paper, we extend Cultural Consensus Theory (CCT) to estimate culturally shared beliefs about faces by incorporating latent constructs structured around interpretable facial features extracted via computer vision algorithms. We apply our model to a large-scale dataset of people’s first impressions of faces. Our approach reveals a robust mapping between facial features and culturally constructed impressions, allowing us …

Photoflow Synthesis And Characterization Of Cis- And Trans-N-Isohumulone, Gregory M. Giovine, Bradley Gallow, Trevor Smith, Bruce Hamper

Undergraduate Research Symposium

Previously published efforts by the Hamper group demonstrated the viability of photochemistry in the isomerization of n-humulone to n-isohumulone. Photochemical isomerization reactions of n-humulone performed under 400nm light over 8 hours resulted in high yields (84% reported) of largely trans-n-isohumulone (>95%). These promising results invited further examination of the photochemical process and the application of photoflow procedure. Herein we report the beginnings of a comprehensive slate of n-humulone isomerization reactions performed under iterative photoflow conditions.

Caffeinated Bees: Investigating The Impact On Foraging Behavior, Taylor Boaz, Becky Hansis-O'Neil, Aimee Dunlap Phd

Undergraduate Research Symposium

Caffeine, a naturally occurring stimulant found in the nectar of some flowering plants (e.g., coffee, citrus), has been shown to affect insect behavior, including learning and memory in bumblebees. The presence of this compound in floral resources suggests a potential role in shaping pollinator foraging strategies. This study investigated the impact of caffeine exposure on the foraging behavior of bumble bees (Bombus impatiens).

Individually tagged bees were trained to forage on an artificial flower wall to establish a baseline foraging pattern. Following training, the bees were isolated and randomly assigned to one of two treatment groups: a control …

Influencing The Meteoric Rise Of Ai: Centering Ethics, Law, And Society In Urgent Policy Reform, Erin L. Shah Mspm, Heath A. Clark Mba, Xarlene B. Choi Bs, Garrett K. Chan Phd

The Guardianship Journal

Artificial intelligence (AI) represents a transformative technological advancement with the potential to yield extraordinary societal benefits while simultaneously introducing significant potential harms to individuals, governments, and society. The adoption of AI has outpaced the ability of society to collectively and cohesively respond through the implementation of adequate regulatory guardrails. While AI presents novel benefits and challenges, the broader dynamic of responding to new, uncharted technologies has historical precedence. The article's methodological approach employed empirical research using law articles and policies. As such, the article explores the benefits, challenges, and actual or potential harms in three business sectors: business and finance, …

Engaging Seminars: The Impact Of Storytelling In Public Safety Education, Mimi Gough, Glenn L. Walton

The Guardianship Journal

This paper examines the important role of storytelling in higher education as a technique for engaging students in course content and applies its relationship to the experiential learning theory (ELT) in which learners are actively seeking knowledge through authentic application. Storytelling can be a particularly effective method when used during live online seminars in public safety education, since this particular program of study often relies on verbally communicating realistic and credible information. Sharing various examples of actual encounters can help students better prepare for these types of situations when they face them in their future careers. Incorporating evidence to support …

"Let Your Body Do The Talkin'": An Investigation Of Aesthetic Experience Through Phenomenological Psychology, Tone Roald, Anders Essom-Stenz

Duquesne Studies in Phenomenology

How does the experience of one’s own body contribute to aesthetic experience? This article answers the question from the point of view of phenomenological psychology by portraying how the body is lived from within in intense experiences with works of visual art. Based on descriptions of aesthetic experiences juxtaposed with phenomenological insights about the body and, in particular, Maurice Merleau-Ponty’s thoughts on concrete and abstract movement, it is shown how the lived body gives form to the experience. The experience of one’s own body reflects the expressions of the works of art and comprises not only movement, and with it …

Participatory Presence In Aesthetic Experiences Of Performance, Sophie Morrissey, Shaun Gallagher

Duquesne Studies in Phenomenology

In the context of the performing arts, presence can generally be thought of as an experienced connection between performer and audience that heightens the affective impact of the performance. Explorations of how presence is experienced during live performance contribute to our understanding of the relation of presence to participants’ overall aesthetic experience and participation in the performance. However, they also reveal the complex phenomenology of presence and the challenge of offering a unified framework that can account for its various phenomenal manifestations. In this article, we conceive of presence as a recursive dynamical feedback loop that emerges through and modulates …

With Images In Time. Atmosphere And Art: Mediation And Dialogue, Pauline Von Bonsdorff

Duquesne Studies in Phenomenology

The article challenges the idea that atmospheres are always sensed immediately. Instead, atmospheres in art are mediated and arise in a dialogue between work and audience. To understand how, we should pay attention to temporal structures in and around the work, such as the dynamics and duration of aesthetic experience and the historicity of both work and perceiver, including pasts and present. Mikel Dufrenne’s phenomenological aesthetics, complemented with insights from Maurice Merleau-Ponty’s analyses of perception, offer resources for this. Writing on expression, Dufrenne distinguishes “presence,” in which sense is felt immediately and firmly connected to embodiment, and “sentiment,” which arises …

Dual-Task Exercise Protects Healthy Older Adults Against In-Creased Risk For Dementia: A Controlled Trial, Helen Tatiane Santos Pontes, João Bento-Torres, Naina Yuki Vieira Jardim, Victor Oliveira Da Costa, Josilayne Patricia Ramos Carvalho, Cristovam Wanderley Picanço-Diniz, Natáli Valim Oliver Bento-Torres

Baltic Journal of Health and Physical Activity

Background: Functionality in aging is closely linked to the ability to divide attention during multitasking activities, which are essential for activities of daily life. However, coordination between motor and cognitive simultaneous tasks decreases with age, impairing functional ability and restricting older adults' participation and healthy aging. This study aimed to assess the effects of a multimodal dual-task exercise intervention on motor and cognitive dual-task costs and its potential to mitigate age-related declines in healthy older adults. Materials and Methods: A controlled trial was conducted with community-dwelling older adults assigned to either the Dual-Task Exercise group, which participated in a 24-session …

'Coming Here, It’S Just Weird': 'Making The Familiar Strange' Through Exploring Experiences Of Sexism And Feminisms, Emilia A I Little

Plymouth Interdisciplinary Education Open Journal

This paper explores personal experiences with gender and how these experiences changed whilst attending university as an undergraduate student. The paper uses gender and feminist theory to guide a critical and auto-ethnographic exploration of personal experiences of a young woman within a patriarchal system. The paper uses journal excerpts to recount past and current experiences through feminist lenses. Through exploring gender theory, binary and performativity, personal experiences are validated and explained. The force of patriarchal oppression and privilege were illuminated as personal conceptions of gender were disrupted. The paper argues for much-needed feminist education in schools, centring around: avoiding the …

The Impact Of Exercise On Albuminuria And Kidney Function In The 2nd And 3rd Trimesters Of Physiological Pregnancy, Wojciech Wołyniec, Andrzej Kochanowicz, Jan Mieszkowski, Łukasz Radzimiński, Marcin Renke, Anna Szumilewicz

Baltic Journal of Health and Physical Activity

Background: Physical activity is an important part of healthy lifestyle and should be continued during low-risk pregnancy. Several studies have suggested that most pregnant women had no contraindications to perform exercises. The aim of the study was to assess the acute effect of an exercise test up to refusal and a chronic impact of an 8-week HIIT program on albuminuria and kidney function in the 2nd and 3rd trimester of pregnancy. Material and Methods: A group of 36 pregnant women was studied. At the beginning of the study, all participants had normal kidney function and albuminuria at rest. Nineteen women …

The Impact Of Absent Fathers In Forensic And Criminal Justice Settings, Sara J. Bradley

Master of Arts in Human Services

This capstone examines the interplay between father absence, marginalization through economics, and larger structural factors in shaping youth crime and intergenerational patterns of risk. This review synthesizes studies in family instability, neighborhood disadvantage, and barriers to paternal involvement in demonstrating the interplay between larger forces in shaping developmental outcomes. Father absence correlates with diminished protective factors, increased exposure to stressful contexts, and elevated dangers of system contact. Larger structural factors of concentrated poverty levels, resource scarcity in neighborhoods, and inconsistent levels of support upon return from incarceration exacerbate dangerous outcomes and intergenerational patterns. The applications analysis in this capstone evaluates …

Impact Of Childhood Abuse And Trauma On Ptsd, Charlie Schultz

Master of Arts in Human Services

Child abuse and trauma is very present in our society today and there is a strong link between child abuse and PTSD. A strong literature review was carefully crafted that highlighted the importance of understanding PTSD and childhood trauma. Chapter two included a major in-depth look at the link between PTSD and childhood trauma and the lasting results. The literature review explained not only how PTSD is linked with childhood trauma, but it also included a really in depth look at PTSD and how it relates to some of the other known mental childhood disorders. The paper explained the major …

Optimization Of Research Pipeline To Characterize The Effects Of Atp-Sensitive Potassium Channels Mutations On Native Skeletal Muscle Fibers And Evaluate Potential Drug Therapy, Yuezhou Chen

McKelvey School of Engineering Graduate Student Theses & Dissertations

The ATP-sensitive potassium (KATP) channel is a critical metabolic sensor in skeletal muscle, yet its definitive molecular composition and functional roles remain contested. In this study, I conclusively demonstrate that the Kir6.2/SUR2 complex forms sarcolemmal KATP channel in mouse fast-twitch muscle. Genetic knock-out (KO) of Kir6.2 (Kcnj11−/−) or SUR2 (Abcc9−/−) resulted in a similar phenotype with increased fatigue resistance and a pathological rise in unstimulated resting tension at high-frequency stimulation. In contrast, SUR1 (Abcc8−/−) KO was ineffective. Furthermore, a CRISPR/Cas9 knock-in mouse model of a human SUR2A truncation variant (KCGV/KCGV) recapitulated the abnormal force accumulation, demonstrating that even loss-of-function of …

Development Of A Multispectral Collagen Detection Dermatology Device For Delineation Of Non-Melanoma Skin Cancers, Anmol Jarang

McKelvey School of Engineering Graduate Student Theses & Dissertations

To diagnose and manage skin disease, clinicians primarily use visual inspection aided with dermatoscopes, which are cross-polarized magnifiers. In recent years, digital cross polarized multispectral imaging systems have been developed to quantitatively assess skin disease. For example, systems like the one developed by Yaroslavsky et al., which utilize red and blue filters, can non-invasively assess the collagen network in the dermis(Yaroslavsky et al., 2003). Visualization of collagen network disruption can make it easier to visualize the boundaries of skin cancers like keratinocyte carcinoma, and therefore easier to excise completely. Current systems use external bandpass filters in front of cameras to …

Expansion Limits Of Meshed Split-Thickness Skin Grafts, Haomin Yu

McKelvey School of Engineering Graduate Student Theses & Dissertations

Split-thickness skin grafts are widely used to treat chronic wounds. Procedure design requires surgeons to predict how much a patch of the patient's own skin expands when it is meshed with rows of slits and stretched over a larger wound area. Accurate prediction of graft expansion remains a challenge, with current models overestimating the actual expansion, leading to suboptimal outcomes. Inspired by the principles of mechanical metamaterials, we developed a model that distinguishes between the kinematic rearrangement of structural elements and their stretching, providing a more accurate prediction of skin graft expansion. Our model was validated against extensive data …

Examining The Role Of Early Bilingualism On Interference Suppression And Prefrontal Connectivity, Matthew L. Cook, Lisa K. Boyce, Allison S. Hancock, Mackenzy S. Turner, Spencer D. Bradshaw

Human Development and Family Studies Student Research

Inhibitory control is a core cognitive function that is primarily associated with activation in the prefrontal cortex (PFC) and is the cognitive function that inhibits impulses, thoughts, and suppresses irrelevant information to an identified goal or task. Prior research suggests that bilingualism may affect brain activity related to inhibitory control, yet few studies have compared functional activity between monolingual and bilingual children. The current study used functional near-infrared spectroscopy (fNIRS) to examine region of interest comparisons and task-state functional connectivity across the PFC during an interference suppression Simon task with 13 bilingual (East Asian or Ibero-romance paired with English) and …

Ratio Quantification Of Geranium And Rose Essential Oil Mixtures Via Leep Learning Analysis Of Complex Raman Spectra, Jia Wei Tang, Yong Xuan Hong, Jie Chen, Yun Yun Xie, Zhang Wen Ma, Qing Hua Liu, Liang Wang

Research outputs 2022 to 2026

Due to their high economic value, essential oils (EOs) are increasingly subject to adulteration, posing significant challenges for quality assessment and control. Owing to its excellent molecular specificity, Raman spectroscopy has been widely employed for the analysis and evaluation of EO products. In this study, we collected a total of 2700 Raman spectra comprising rose essential oil (REO, n = 900), geranium essential oil (GEO, n = 900), and their mixtures (n = 900). We first constructed a conventional convolutional neural network to distinguish spectra corresponding to varying EO mixing ratios. Interpretability analysis revealed that spectral peaks at 800, 1000, …

Osteoprogenitor Cells From Non-Regenerative Bone Show Greater Resistance To Cellular Stress Than Those From Regenerative Bone, Joshua Broussard, Sylvia Culpepper, Tyrel Long, Alexander J. Trostle, Robert J. Tower, Mimi C. Sammarco, Jennifer Simkin

School of Medicine Faculty Publications

INTRODUCTION: Bone regeneration following injury depends on osteoprogenitor cells derived predominantly from the periosteum. Incomplete regeneration has been attributed to both cell-extrinsic factors (e.g., environment, inflammation, mechanical instability) and cell-intrinsic factors (e.g., impaired proliferation or differentiation of stem cells). In the digit amputation mouse model, amputation through the third phalanx (P3) supports complete regeneration, while amputation through the second phalanx (P2) results in callus formation and scarring. Periosteal cells are known to be the major contributing cell source for repair and regeneration. Yet the healing outcomes of P2 and P3 amputations are significantly different. This study tests whether P2 and …

Trauma-Informed Training And Practices For Elementary School Lunchroom Supervisors, Heather C. Kostohryz

Dissertations, Theses, and Projects

School lunchrooms often see behavior disruptions at a higher rate than other areas within a school. Of the numerous reasons as to why, the inadequate training of those who supervise the lunchroom can be remedied easier than other factors (Wheatley et al., 2009). Over the past ten years a growing recommendation is for all staff to be included in school-wide behavior management and training, and that the training be focused on trauma-informed practices as a way to aid all students (Nealy-Oparah & Scruggs-Hussein, 2018). As 68% of children will experience some form of trauma during childhood, it stands to reason …

The Challenges Of International Maritime Treaty Enforcement: Examining Outcomes Of The South Sea And Red Sea Dispute, Sofia Roshan Hope Gellada

Senior Theses

The challenges of enforcing international treaty obligations are explored through the lens of the United Nations Convention on the Law of the Sea (UNCLOS) and its newly introduced addendum, the Biodiversity Beyond National Jurisdiction (BBNJ) Agreement. Through a detailed analysis of two case studies—the South China Sea Dispute and the Red Sea Dispute—this paper highlights the significant obstacles to treaty enforcement, particularly in contexts of power imbalances. Both disputes, arbitrated by the Permanent Court of Arbitration, reveal a troubling pattern of non-compliance by stronger states, such as China and Eritrea, despite legally binding rulings. These findings underscore the limitations of …

The Feasibility Of Transaldolase Inhibitors As Anti-Cancer Therapeutics, Daniel Scot Baer

Chemistry and Biochemistry

Metabolism was a major focus of cancer research 100 years ago, but its prevalence declined in the latter part of the twentieth century. In the last several decades, however, it has become clear that cancer cells frequently modify their metabolic programs to enable both their survival and rapid proliferation. Transaldolase (TA), an enzyme within the pentose phosphate pathway, contributes to these outcomes when its activity is increased, yet for a variety of reasons inhibition of the enzyme has remained comparatively understudied. Since TA flux enables nucleotide production and high levels of NADPH synthesis, aberrant, cancer-causing signaling pathways often increase expression …

Towards Science-Based Quantitative Conservation Management Plan Add-Ons For All Alaskan Squirrel Species: Current And Future Distributions, Conservation Management, Contaminant Exposure, And Their Role In Alaska Native Communities, Moriz Steiner

Biological Sciences

Squirrels are widespread in Alaska, yet a science-based conservation management plan (CMP) is absent at the state level. Squirrels are killed year-round with no bag limits, while population metrics remain unknown and conservation statuses disputed. This thesis proposes modern tools and approaches as addons for long-term, sustainable, holistic CMPs for all Alaskan squirrel species in a changing climate. Chapter 1 provides an overview of squirrels' current roles and uses in Alaska alongside existing management practices and regulations. Chapters 2 and 3 conduct thorough conservation assessments by analyzing and predicting current and future distributions of squirrel species across Alaska and a …

Determination Of The Cecal Microbiota Profile Of Anadolu-T Broiler Breeder Chickens Using 16s Metagenomic Sequencing, Kürşat Teti̇k, İsmai̇l Özkan, Serpi̇l Kahya Demi̇rbi̇lek

Turkish Journal of Veterinary & Animal Sciences

The gut microbiota has significant effects on the performance, health status, and immune systems of chickens. The complex microbial community structure begins to form after chicks hatch with the intake of feed, water, and air into the digestive system. Disrupting the balance of this system can lead to the proliferation of pathogenic microorganisms. Until recently, studies on microbiota relied on traditional microbiological techniques that cultured only a small fraction of the microbial diversity. However, significant advancements in DNA sequencing and bioinformatics have enabled the identification of diverse bacterial populations in environmental samples. In this study, a total of 2000 broiler …

Parameter Optimization For Runs Of Homozygosity (Roh) Detection In The Pig Genome, Timofey Romanets, Anna Korobeynikova, Lyubov Getmantseva, Maria Kolosova, Elena Romanets, Ilona Bakoeva, Varvara Shevtsova, Anatoly Kolosov, Alexander Maksimov, Siroj Bakoev

Turkish Journal of Veterinary & Animal Sciences

The present study analyses the influence of various parameters on the accuracy and reliability of ROH identification procedures, as well as their relationship with inbreeding and genetic diversity in Duroc pig populations. The genotyping data of 70 Duroc pigs obtained using PorcineSNP80v1 chips were used, and the effects of LD pruning and ROH calling parameters were evaluated. It was found that the use of strict basic filters during the execution of the --homozyg command reduces the likelihood of false positives and contributes to the more accurate identification of ROH. The results show that LD pruning reduces the detection of random …

Differences In Lactation Curve Parameters And Milk Yield Responses Between Artificially Induced And Naturally Calved Cows Milked In An Automatic Milking System, Marcos Busanello

Turkish Journal of Veterinary & Animal Sciences

Despite advances in artificial induction of lactation (AIL) protocols, they do not perfectly mimic the hormonal patterns of natural pregnancy. Recent studies show improved milk yield (MY) responses; however, comparisons of lactation curve parameters between AIL and naturally calved (NC) cows often do not extend beyond 305 days in milk (DIM). Thus, this study aimed to compare MY responses and lactation curve parameters between AIL and NC cows milked in an automated milking system (AMS). Data from 38 Holstein cows (19 AIL and 19 NC) were analyzed, focusing on the 305 DIM period. Wood’s lactation curve model was used to …

Endocrine Disruptors, Insulin Resistance, And Diabetes, Meri̇ç Coşkun, Ethem Turgay Ceri̇t, Tuğba Barlas, Alev Eroğlu Altinova

Turkish Journal of Medical Sciences

Endocrine-disrupting chemicals (EDCs) are compounds that interfere with hormone synthesis, secretion, metabolism, or excretion. Evidence indicates that increased exposure to EDCs is associated with insulin resistance and, most notably, type 2 diabetes worldwide. This suggests a diabetogenic effect that is independent of obesity, underscoring the complex mechanisms and broad impact of EDCs on metabolic health. Key pathways include hormone mimicry and antagonism, altered hormone metabolism, inflammatory responses, and mitochondrial dysfunction. This review summarises the mechanisms through which EDCs contribute to these conditions and evaluates the epidemiological and experimental evidence supporting these associations.