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Crow's Nest, November 22, 2021, Crow's Nest Staff

Crow's Nest

The November 22, 2021, issue of the Crow's Nest.

Oligomerization Of Mutant P53 R273h Is Not Required For Gain-Of-Function Chromatin Associated Activities, George K. Annor, Nour Elshabassy, Devon Lundine, Don-Gerard Conde, Gu Xiao, Viola Ellison, Jill Bargonetti

Publications and Research

The TP53 gene is often mutated in cancer, with missense mutations found in the central DNA binding domain, and less often in the C-terminal oligomerization domain (OD). These types of mutations are found in patients with the rare inherited cancer predisposition disorder called Li-Fraumeni syndrome. We previously found that mutant p53 (mtp53) R273H associates with replicating DNA and promotes the chromatin association of replication-associated proteins mini-chromosome maintenance 2 (MCM2), and poly ADP-ribose polymerase 1(PARP1). Herein, we created dual mutants in order to test if the oligomerization state of mtp53 R273H played a role in chromatin binding oncogenic gain-of-function (GOF) activities. …

Influence Of Surfactant-Humectant Adjuvants On Physical Properties, Droplet Size, And Efficacy Of Glufosinate Formulations, Estefania G. Polli, Guilherme S. Alves, Jesaelen Gizotti De Moraes, Greg Robert Kruger

Department of Agronomy and Horticulture: Faculty Publications

Glufosinate efficacy is inconsistent among weed species and under environmental conditions that favor rapid droplet drying. Surfactant-humectant adjuvants could maximize glufosinate efficacy by increasing wetting and penetration into the leaf surface while decreasing evaporation rate (ER). However, there is a lack of information in the literature about the interaction of surfactant-humectants adjuvants with glufosinate. The objective of this study was to investigate the influence of surfactanthumectant adjuvants on the physical properties, droplet size, and efficacy of two glufosinate formulations. Laboratory, greenhouse, and field studies were conducted at the Pesticide Application Technology Laboratory of the University of Nebraska- Lincoln. Treatment design …

Dynamic Ph And Thermal Analysis Of Paper-Based Microchip Electrophoresis, Muhammad Noman Hasan, Ran An, Asya Akkus, Chirag Kharangate, Umut A. Gurkan

Faculty Scholarship

Paper-based microchip electrophoresis has the potential to bring laboratory electrophoresis tests to the point of need. However, high electric potential and current values induce pH and temperature shifts, which may affect biomolecule electrophoretic mobility thus decrease test reproducibility and accuracy of paper-based microfluidic electrophoresis. We have previously developed a microchip electrophoresis system, HemeChip, which has the capability of providing low-cost, rapid, reproducible, and accurate point-of-care (POC) electrophoresis tests for hemoglobin analysis. Here, we report the methodologies we implemented for characterizing HemeChip system pH and temperature during the development process, including utilizing commercially available universal pH indicator and digital camera pH …

Opposing Community Assembly Patterns For Dominant And Nondominant Plant Species In Herbaceous Ecosystems Globally, Carlos Alberto Arnillas, Elizabeth T. Borer, Eric W. Seabloom, Juan Alberti, Selene Baez, Jonathan D. Bakker, Elizabeth H. Boughton, Yvonne M. Buckley, Miguel Nuno Bugalho, Ian Donohue, John Dwyer, Jennifer Firn, Riley Gridzak, Nicole Hagenah, Yann Hautier, Aveliina Helm, Anke Jentsch, Johannes M. H. Knops, Kimberly J. Komatsu, Lauri Laanisto, Rebecca L. Mcculley

Plant and Soil Sciences Faculty Publications

Biotic and abiotic factors interact with dominant plants—the locally most frequent or with the largest coverage—and nondominant plants differently, partially because dominant plants modify the environment where nondominant plants grow. For instance, if dominant plants compete strongly, they will deplete most resources, forcing nondominant plants into a narrower niche space. Conversely, if dominant plants are constrained by the environment, they might not exhaust available resources but instead may ameliorate environmental stressors that usually limit nondominants. Hence, the nature of interactions among nondominant species could be modified by dominant species. Furthermore, these differences could translate into a disparity in the phylogenetic …

The Dietary Inflammatory Index Is Not Associated With Gut Permeability Or Biomarkers Of Systemic Inflammation In Hiv Immunologic Non-Responders, Fat Malazogu, Rodney K. Rousseau, Nitin Shivappa Mbbs, Mph, Ph.D., Sanja Huibner, Sharon L. Walmsley, Colin M. Kovacs, Erika Benko, Robert J. Reinhard, Ron Rosenes, James R. Hébert, Rupert Kaul

Faculty Publications

Immunologic non-responders (INRs) are a subset of individuals living with HIV who have suboptimal blood CD4+ T cell recovery despite effective antiretroviral therapy (ART). They are at an increased risk of serious non-AIDS co-morbidities and death, and demonstrate enhanced systemic immune activation. In other populations diet has been correlated with markers of systemic inflammation through the Diet Inflammatory Index (DII), but this association has not been studied in persons living with HIV (PLWH). Blood was collected from 28 INR PLWH with a blood CD4+ T cell count <350/μL despite ≥2 years of effective ART. Participants completed a Canadian Diet History Questionnaire, and their responses were used to calculate the DII. Plasma inflammatory markers (IFNγ, TNF, IL-6, sVCAM, D-dimer, sCD14 and CRP) were assayed by ELISA, cellular immune activation (HLA-DR and CD38 on CD4+ and CD8+ T cells) was quantified using flow cytometry, and small bowel permeability assessed by calculation of the urine LacMan ratio after drinking a mix of lactulose and mannitol. Participants were a median age of 57 years, had been on effective ART for 15 years, and the median DII was −1.91 (range of −3.78 to +2.23). No correlation was observed between DII and plasma markers of inflammation, levels of T cell activation, gut permeability, or the biomarker of bacterial translocation sCD14. Self-reported alcohol intake, a potential confounder of the relationship between diet and inflammatory biomarkers, was also not associated with systemic inflammation or gut permeability. Our findings suggest that other mechanisms, rather than diet, are likely to be the major driver of systemic inflammation in INR individuals.

Proving The Proverbial Gadfly: Situating The Historical And Racial Context Of Southern Medical Works By Mary Louise Marshall, Aidybert Weeks

Library Faculty Research

Health sciences librarianship has historically benefited from avoiding critical conversations around the role of race in the profession, reflected through a select few number of articles on the topic. The purpose of this study was to add to this body of literature and apply a critical librarianship framework on the early scholarly record of health sciences librarianship and the legacy of integration within the Medical Library Association (MLA). Three Southern medical works and the integration views of Mary Louise Marshall, the longest-serving president of MLA from 1941 to 1946, were thematically and textually analyzed to redress the profession’s long-standing legacy …

Buhrmann, Haywood Honored By Mclean County Ywca With "Women Of Distinction" Awards, Kailee Galloway

News and Events (Discontinued Series)

No abstract provided.

Applying The Nominal Group Technique For Specifying The Interacting Dimensions Affecting Adherence To Post–Sharps Injury Follow-Up Services, Abdullah Aliff Abdul Wahab, Rosnah Ismail, Asnarulkhadi Abu Samah, Noor Dalila Inche Zainal Abidin, Nurul Asyikin Mustaffa, Halim Ismail

The Qualitative Report

Scale of adherence to post–sharps injury follow-up services among healthcare workers is uncommon compared to medication adherence scale. To develop a novel and culturally adapted scale, stakeholders should be consulted to specify dimensions by deducing it from the existing framework. This study was to demonstrate how health stakeholders were consulted to specify the dimensions.

This study employed two sessions of Nominal Group Technique. Each session consisted of 12 purposive-sampled mixed participants i.e., healthcare managers and providers. A four-step sequential protocol was used for collecting participants’ key ideas on what adherence factors in post–sharps injury follow-up services: silent idea generation, round-robin …

Triophaser: Using Mendelian Inheritance Logic To Improve Genomic Phasing Of Trios, Dustin B. Miller, Stephen R. Piccolo

Faculty Publications

Background: When analyzing DNA sequence data of an individual, knowing which nucleotide was inherited from each parent can be benefcial when trying to identify certain types of DNA variants. Mendelian inheritance logic can be used to accurately phase (haplotype) the majority (67–83%) of an individual’s heterozygous nucleotide positions when genotypes are available for both parents (trio). However, when all members of a trio are heterozygous at a position, Mendelian inheritance logic cannot be used to phase. For such positions, a computational phasing algorithm can be used. Existing phasing algorithms use a haplotype reference panel, sequencing reads, and/or parental genotypes …

The Interface Between Cell Signaling Pathways And Pregnane X Receptor, Robert S. Rogers, Annemarie Parker, Phill D. Vainer, Elijah Elliott, Dakota Sudbeck, Kaushal Parimi, Venkata P. Peddada, Parker G. Howe, Nick D'Ambrosio, Gregory Ruddy, Kaitlin Stackable, Megan Carney, Lauren Martin, Thomas Osterholt, Jeff Staudinger

Faculty and Staff Publications

Highly expressed in the enterohepatic system, pregnane X receptor (PXR, NR1I2) is a well-characterized nuclear receptor (NR) that regulates the expression of genes in the liver and intestines that encode key drug metabolizing enzymes and drug transporter proteins in mammals. The net effect of PXR activation is to increase metabolism and clear drugs and xenobiotics from the body, producing a protective effect and mediating clinically significant drug interaction in patients on combination therapy. The complete understanding of PXR biology is thus important for the development of safe and effective therapeutic strategies. Furthermore, PXR activation is now known to specifically transrepress …

Pre-Sowing Treatments Improve Germinability Of South Texas Native Plant Seeds, Kaitlynn Lavallee, Pushpa Soti, Hansapani Rodrigo, Rupesh R. Kariyat, Alexis Racelis

School of Earth, Environmental, & Marine Sciences Faculty Publications

The incorporation of native plant species is central to restoration efforts, but this is often limited by both the availability of seeds and the relatively low viability and germination rates of commercially available seeds. Although pre-sowing treatments are commonly used to improve germination rates of seeds, the efficacy of these treatments is found to vary across species. In this study, we tested how four pre-sow treatments (physical scarification, acid scarification, cold stratification, and aerated hydropriming) affected the viability and seed germination rates of 12 commercially available plant species native to south Texas and commonly used in restoration efforts. Our results …

Clinical Trial Of Abcb5+ Mesenchymal Stem Cells For Recessive Dystrophic Epidermolysis Bullosa, Dimitra Kiritsi, Kathrin Dieter, Elke Niebergall-Roth, Silvia Fluhr, Cristina Daniele, Jasmina Esterlechner, Samar Sadeghi, Seda Ballikaya, Leoni Erdinger, Franziska Schauer, Stella Gewert, Martin Laimer, Johann W. Bauer, Alain Hovnanian, Giovanna Zambruno, May El Hachem, Emmanuelle Bourrat, Maria Papanikolaou, Gabriela Petrof, Sophie Kitzmüller, Christen L. Ebens, Markus H. Frank, Natasha Y. Frank, Christoph Ganss, Anna E. Martinez, John A. Mcgrath, Jakub Tolar, Mark A. Kluth

Research outputs 2014 to 2021

BACKGROUND. Recessive dystrophic epidermolysis bullosa (RDEB) is a rare, devastating, and lifethreatening inherited skin fragility disorder that comes about due to a lack of functional type VII collagen, for which no effective therapy exists. ABCB5+ dermal mesenchymal stem cells (ABCB5+ MSCs) possess immunomodulatory, inflammation-dampening, and tissue-healing capacities. In a Col7a1-/-mouse model of RDEB, treatment with ABCB5+ MSCs markedly extended the animals' lifespans. METHODS. In this international, multicentric, single-arm, phase I/IIa clinical trial, 16 patients (aged 4-36 years) enrolled into 4 age cohorts received 3 i.v. infusions of 2 × 106ABCB5+ MSCs/kg on days 0, 17, and 35. Patients were followed …

Growth And Development Of Research Data Management In India: A Visual Analysis Of Published Literature Indexed In Scopus, Manu T R, Bhakti Gala

Library Philosophy and Practice (e-journal)

The present paper identifies and presents a visual analysis of Research Data Management (RDM) literature published by Indian researchers and indexed in Scopus – the bibliographic and citation database. The researchers have used standard bibliometric methods of measurements to conduct the study such as Bradford’s law, Lotka’s law and Zipf’s Law among other parameters. The study findings indicate that India is rapidly focusing on RDM with its contribution of 136 publications to the global output of 4929 publications over a period of 1945 to 2021, ranking 12^th globally. Out of 136 publications, 35.29% (48) were published as open access …

Prognosis Of Breast Cancer Patients And Survival Rate Related With Ki-67 Proliferation Index: Multicenter Retrospective Study, Sanjay D. Dandona, Samarasimha Ganeshkumar

Muthanna Medical Journal

Breast cancer commonest cancer of urban Indian women and the second commonest in the rural women. It has different epidemio-clinical characteristics compared with European countries. The purpose of this study is to investigate prognosis and survival rate of breast cancer patients related with Ki-67 proliferation index in multicenter through retrospective study. We reviewed all patient’s data in registration center of Medical Oncology Departments, in the period 2016-2020. Data has been collected on 760 breast cancer patients. Our resulted data showed most prevalent comorbidities associated with breast cancer were cardiovascular diseases, diabetes and anemia. Ki-67 index >28% mostly associated with early …

Urine Calcium But Not Plasma Calcium Or Urine Hydroxyproline Is Increased By A Systemic Acidosis In The Dairy Cow, J. R. Roche, Dawn E. Dalley, F. O’Mara, E. S. Kolver

IGC Proceedings (1977-2023)

Eight non-lactating, pregnant Holstein-Friesian cows were allocated to two treatments and individually offered diets differing in dietary cation-anion difference. Decreasing the dietary cation-anion difference reduced the urine pH within hours of anionic salt supplementation. Plasma calcium concentration was unaffected by dietary cation-anion difference but urine calcium concentration was significantly increased within 10 days of including anionic salts in the diet. Faecal calcium concentration was significantly reduced, indicating increased calcium absorption. Dietary calcium concentration or dietary cationanion difference did not significantly affect urinary hydroxyproline.

Dry Matter Intake Of Periparturient Cows On A Fresh Pasture/Pasture-Hay Diet, J. R. Roche, Dawn E. Dalley, F. O’Mara, E. S. Kolver

IGC Proceedings (1977-2023)

Reports of the depression in dry matter intake in the periparturient period have been inconsistent and little dry matter intake data is available for an all-forage diet prior to calving. Data to examine intake depression prior to calving was obtained from two experiments. In experiment 1, sixteen non-lactating, periparturient cows ate 1.3% of pre-calving body weight of grass-hay and freshly cut grass for two weeks pre-calving (restricted). Experiment 2 comprised thiry-six cows that ate 1.6% of pre-calving body weight of grass-hay and freshly cut grass for the final two weeks of pregnancy (ad libitum). Individual dry matter intakes …

Effectt Of Supplements On Forage Degradability Of Brachiaria Brizantha Cv. Marandu Grazed By Steers, J. R. A. Pereira, Ricardo A. Reis, L. R. De A. Rodrigues, D. Freitas

IGC Proceedings (1977-2023)

The supplementation effect was evaluated using crossbred rumen-fistulated steers maintained in a Brachiaria brizantha cv. marandu pasture. The animals received energy supplementation (corn-COR); low degradability protein supplementation (corn gluten-GLU); and high degradability protein supplementation (soybean-SBM) at two levels N1 (± 0.5 kg/an/day) and N2 (± 1.5 kg/an/day); or no supplementation (Control). The factorial (3x2), plus the control, was studied according to complete randomized block design with three replications. The supplementation did not affect (P> 0.05) the potential degradability (POD), the effective degradability (EFD), and the degradation rates (DR) of the forage (extrusa). The GLU supplementation provided higher (P< 0.05) EFD of the NIDN fraction than the SBM, and both did not differ (P> 0.05) of …

Use Of Supplements For Increasing Performances Of Suckling Martinik Ewes When Fed Tropical Forages, E. O. Jimenez, G. Alexandre, H. Archimède, P. Despois, M. Jean-Bart, C. Jacquet

IGC Proceedings (1977-2023)

Experiments have been carried out to determine the effects of supplementation upon milk production and growing performances with hair sheep Martinik ewes weighing 48 ± 4 kg liveweight (LW). In a first trial, 6 single bearing ewes (group GS) were offered individually a daily 0.5 kg of commercial pellets. They were compared to the control group (GN; 6 ewes) with no supplement during 10 weeks of lactation. Basal diet was chopped Digitaria decumbens grass ad libitum. In a second trial, level of supplementation was adapted to the ewes’ litter size: 6 twins bearing ewes (TW) and 6 single (SI). …

Tactical Circuit Training Improves Blood Pressure And Vascular Health More Than Resistance Training, Kelly C. Mclaughlin, Avery N. Perez, Stephanie Donahue, Deborah L. Feairheller

International Journal of Exercise Science

International Journal of Exercise Science 14(3): 1320-1333, 2021. Exercise training is known to reduce CVD risk factors; however, in tactical populations, like veterans and firefighters, the effects of different forms of exercise such as tactical circuit training (CT) or conventional resistance training (RT) is unclear. Thus, the purpose of this study was to compare changes in various CVD risk measures after 4-week tactical CT or RT programs. Thirty-seven firefighters (20 CT, 17 RT), 35% of whom were veterans, participated. Pre- and post-intervention measures included body fat (BF%), carotid artery intima media thickness (IMT), central and brachial BP, and indices of …

Some Nonparametric Hybrid Predictive Models : Asymptotic Properties And Applications., Tanujit Chakraborty Dr.

Doctoral Theses

Prediction problems like classification, regression, and time series forecasting have always attracted both the statisticians and computer scientists worldwide to take up the challenges of data science and implementation of complicated models using modern computing facilities. But most traditional statistical and machine learning models assume the available data to be well-behaved in terms of the presence of a full set of essential features, equal size of classes, and stationary data structures in all data instances, etc. Practical data sets from the domain of business analytics, process and quality control, software reliability, and macroeconomics, to name a few, suffer from various …

User Role In Boosting Landscape Positive Effect., Asmaa Elantary

Mansoura Engineering Journal

Many studies agree that communication and living within landscape have positive impacts that are reflected on the members of the society and the environment. To avoid the destruction of landscape, the community cooperation always stimulates the human essence in promoting and developing easy and sustainable environmental actions. Sometimes this strong relationship between humans and the environment is not clear and prominent. This research will focus on elucidating this relationship. Therefore, the study aims to reduce the negative effects on the landscape and to shed the light on how people may destroy or save the environment. The study explains solutions of: …

Stratification Of Happiness In Urban Areas In Mexico: A Qualitative Examination By Level Of Marginalization, Oscar A. Martínez-Martínez Professor, Javier Reyes, Eder Noda

The Qualitative Report

Although Mexico presents high levels of poverty and marginalization, it is the second happiest nation in Latin America. This raises several questions about what factors are associated with happiness at each level of marginalization and how these factors vary according to marginalization levels. We conducted a qualitative study in urban municipalities in four Mexican states, using 184 semi-structured interviews and employing a thematic analysis approach. Results suggest that happiness is a multifactorial phenomenon. Factors such as the family, health, religion, friendships, economic conditions, and fulfillment of basic needs contribute to happiness, but each of these aspects has different importance and …

Guidance For Management Of Free-Roaming Community Cats: A Bioeconomic Analysis, Valeria A. Benka, John D. Boone, Philip S. Miller, Joyce R. Briggs, Aaron M. Anderson, Christopher Slootmaker, Margaret Slater, Julie K. Levy, Felicia B. Nutter, Stephen Zawistowski

Publications and Research

Objectives This study used computer simulation modeling to estimate and compare costs of different free-roaming cat (FRC) management options (lethal and non-lethal removal, trap–neuter–return, combinations of these options and no action) and their ability to reduce FRC population abundance in open demographic settings. The findings provide a resource for selecting management approaches that are well matched for specific communities, goals and timelines, and they represent use of best available science to address FRC issues.

Methods Multiple FRC management approaches were simulated at varying intensities using a stochastic individual- based model in the software package Vortex. Itemized costs were obtained from …

Identification Of Needs Of Community Schools Of Kavre, Nepal For Educational Improvement, Roshani Rajbanshi, Parbati Dhungana, Bal Luitel

The Qualitative Report

Needs assessment is a method to find out the current needs of the school that can be considered for providing quality education to the students. This paper presents the needs of five community schools intending to bring changes in the school community. Thus, this paper aims to identify the needs of the five community schools of Kavre, Nepal, and recommend possible interventions. In-depth interviews, observations, and focus group discussions with different stakeholders provided rich data for this study. School-specific needs were identified from the data based on which recommendations are made for intervention, which are: Teacher professional development, contextualized curriculum, …

Sexual Orientation & Gender Identity In International Human Rights Law: Existing Between The Lines, Mika D'Angelo

Cardozo International & Comparative Law Review (CICLR) Blog

There is no international treaty that directly acknowledges the rights of LGBTIQ+ persons or the right to protection from discrimination or violence on the basis of sexual orientation or gender identity. The International Bill of Rights concerns itself with the protection of persons on the basis of “race, colour, sex, language, religion, political or other opinion, national or social origin, property, birth or other status.” This same language is found in Article 2 of the Universal Declaration of Human Rights (UDHR), the International Covenant on Economic, Social and Cultural Rights (ICESCR), and the International Covenant on Civil and Political Rights …

The Danov Archive, Victor Fet, Alexander Kogan

Open Access Books

This memorial volume compiles materials about Rostislav Dаnov (1941—1993) of St. Petersburg (Russia), a naturalist, artist, and snake-hunter, who worked for many years in Turkmenistan (West Kopetgagh Mts). The book celebrates Dаnov’s 80th birthday. It includes previously unpublished artwork and scientific illustrations by Dаnov as well as his texts, research papers, various memoirs, biographic information, and unique photographs. The book is intended for everyone who is interested in history of science and conservation in Central Asia and the USSR.

Фет В, Коган А, составители. Дановcкий архив. Библиотека Университета Маршалла, Хантингтон, Западная Виргиния, 2021. 494 с. Этот том содержит материалы о …

Performance Of Dairy Goats To Alfalfa Silage Based Diets Supplemented With Different Sources Of Carbohydrates, J. L. Danelón, P. Allocati, M. Wawrzkiewicz, S. Bedogne, E. Ceballos, M. D’Alessio, L. Barletta, C. Colatto, D. Victoria

IGC Proceedings (1977-2023)

Lactating Saanen dairy goats fed alfalfa silage (AS) based diets in four 4x4 Latin Square designed experiment were studied for the effects of supplementation of three different type of carbohydrates (wheat grain, (W); sorghum grain, (SG) and dry citrus pulp, (DCP)) on milk yield, composition and chewing activities. Sixteen does (45± 10 DIM and 2.016 kg ± 0.48 4% FCM) housed indoors in individual pens in a four 4x4 experiment were fed four diets 1) AS (33.9%DM, 19.9%CP, 44.01%NDF); 2) ASW (52.4%DM, 19.8%CP, 33.7%NDF); 3) ASSG (50.9%DM, 17.9%CP, 37%NDF), 4) ASDCP (52.5%DM, 16.12%CP, 39.1%NDF) with forage-to-concentrate ratios of 100:0 or …

Integrating Pastures Into The Traditional Slash-And-Burn Cycle In Northeastern Pará, Brazil, S. Hohnwald, B. Rischkowsky, R. Schultze-Kraft, J. M. King, A. P. Camarão

IGC Proceedings (1977-2023)

A project to test possibilities to integrate pastures into the traditional slash-and-burn cycle of small farmers in the Bragantina region in northeastern Pará, Brazil, is presented. Whereas in practice the traditional grass-only pasture is managed separately from the crop/fallow cycle, two options for integration are tested: a Brachiaria humidicola pasture enriched with two bushy and one herbaceous legume, Cratylia argentea, Chamaecrista rotundifolia and Arachis pintoi, and a B. humidicola pasture allowing a controlled regrowth of secondary vegetation ("Capoeira"). The regeneration performance of these pastures for a subsequent cropping period is compared with plots of undisturbed regrowth of Capoeira …

Mineral Concentration Of Herbage From Three Paulownia Species Used For Goat Browse, J-M. Luginbuhl, J. P. Mueller, B. A. Bergmann

IGC Proceedings (1977-2023)

Goats naturally prefer a high proportion of browse in their diets. Therefore, research was initiated to investigate various silvo-pastoral production systems. In May 1997, six Paulownia treatments were planted in a randomized complete block experiment with six replications. Trees were between 6 and 12 cm in height at planting. Treatments included: P. fortunei seedlings, P. tomentosa seedlings, P. elongata seedlings, and three P. elongata clones. Each 4-m wide plot contained a single row of 12 trees with an intra-row spacing of 1 m. Leaf samples were analyzed from all six replications to determine mineral concentration in October 1997, and June …