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Effects Of Depression And Stress On Oral Self-Care Among Perinatal Women In Appalachia: A Longitudinal Study, Jeongwi An

Graduate Theses, Dissertations, and Problem Reports (ETD)

Oral health during the perinatal period has an impact on the health of women and their offspring. Oral self-care behaviors are preventive methods that can be employed to maintain or improve oral health but may be compromised during the perinatal period. Identifying factors affecting oral self-care behaviors might provide direction on how to intervene in oral health during and after pregnancy. The purpose of this study was to investigate independent and interactive effects of depression and stress on oral self-care behaviors of perinatal women in Appalachia, given the burden of oral disease in this region. A total of 1,172 women …

Feed Conditioning Effects On Enzymes, Amino Acids, And Subsequent Broiler Performance, Elizabeth Ann Lynch

Graduate Theses, Dissertations, and Problem Reports (ETD)

There are many things that can be done to improve broiler performance including, but not limited to, pelleting, proper Lys inclusion, and exogenous enzyme supplementation. Pelleting has the ability to improve feed form, reduce wastage, improve bird performance metrics, and decrease pathogens within the feed. Pathogen reduction can be further reduced by hygienic pelleting practices which include an increase in temperature or retention time within a conditioner or hygieniser. However, hygienic pelleting can degrade valuable AA and enzymes (more specifically for the current thesis research Lys and phytase) and make them unavailable to the animal. Supplementing Lys to account for …

Sacramental Ethnicity: Women’S Culture And Vernacular Religion In Twentieth-Century America, Aaron J. Rovan

Graduate Theses, Dissertations, and Problem Reports (ETD)

This project examines the reciprocal and evolving relationship between American women’s culture, vernacular religion, and the social development of American ethnicity. This project focuses on the roles of white ethnic women, both literary and real, in the construction, maintenance, and transmission of ethnic identity. The project highlights the connections between the folkloric performances of vernacular religion and the discursive articulation of ethnicity by focusing on two women writers and two groups of Slovak American women. The fiction of Kate Chopin and Anzia Yezierska illustrates how literary authors bring their contemporary concepts of folklore into their writing. The writings of these …

Learning Mathematics While Black In Rural Appalachia: Black Students' Counterstories And Freedom Dreams About Mathematics Education, Sean P. Freeland

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation aims to illuminate and uncover the experiences of Black students’ learning mathematics in rural Appalachia and specifically West Virginia. The focal theory for this study is Critical Race Theory (CRT) which centers the experience of Black students and their voices. The intersection of race, mathematics education, and the context of rural Appalachia contribute to the analysis of these experiences in specific ways. Participants for this study included six Black high school students from various communities throughout West Virginia. Through interviews and mathematical autobiographies, these students shared their experiences learning mathematics across their schooling experiences and also considering their …

Factors Associated With Service Utilization In Injured Workers In West Virginia, Brandy Brown

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Minimal research has been conducted on healthcare utilization among injured workers residing in rural areas. These workers are often at high risk for work-related injury due to the industries in which they work and commonly experience barriers to accessing healthcare. The first study in this dissertation series explored general characteristics of injured workers receiving care at occupational medicine clinics in West Virginia (WV). The second and third studies explored the moderating effects of rurality, pain medication, and psychiatric medication on sociodemographic and health status factors and number of medical encounters.

Methods: Each study used a cross-sectional design with data …

Frayed Connections: How Long-Term Nitrogen Additions Disrupt Plant-Soil Interactions And The Carbon Cycle Of A Temperate Forest, Brooke A. Eastman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forests are expected to mitigate some of the negative effects of climate change by sequestering anthropogenic carbon (C) from the atmosphere, but the degree to which they drawn down C will depend on the availability of key nutrients, such as nitrogen (N). There is a fair amount of uncertainty in the future of the forest C sink, mostly owing to the fate of soil organic matter (SOM) and soil heterotrophic respiration to future conditions. In N limited systems, plants allocate a significant amount of their photosynthate belowground for the acquisition of nutrients, but under conditions of chronic N deposition, plants …

Capillary Electrophoresis: Method Development And Application In Biomolecule Analysis, Lindsay Marie Veltri

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Capillary Electrophoresis: Method Development and Application in Biomolecule Analysis

Lindsay Veltri

Biomolecule analysis is essential in disease diagnostics and the development of therapeutic agents. There is a vast array of methods available for biomolecule analysis, however, capillary electrophoresis is well-suited due to minimal sample consumption (~nanoliters of solution) and fast analysis times. In this report, capillary electrophoresis was used to analyze glycosylation of therapeutic proteins. Additionally, a capillary electrophoresis-mass spectrometry method is reported for the analysis of proteins.

Post-translation protein modifications like glycosylation, play an important in protein structure and function. Modifications of protein glycosylation can lead to improved …

Critical Perspectives On Produce Prescription Programs & Us Federal Nutrition Policy, Alanna K. Higgins

Graduate Theses, Dissertations, and Problem Reports (ETD)

Produce prescription programs, interventions operating at the intersection of food access and public health, are steadily increasing in number across the United States since 2010. I leverage key informant interviews, participant observations, and event ethnographies to form a four-year institutional ethnography of the implementation of produce prescriptions within West Virginia alongside a legal-policy archaeology methodology to understand how produce prescriptions have been institutionalized and funded within the US Farm Bill. While much of produce prescription program growth is attributed to an expansion of federal funding starting in 2014, this dissertation demonstrates that these programs and the federal legislation which has …

Amelioration Of Mitochondrial Bioenergetic Dysfunction In Diabetes Mellitus: Delving Into Specialized And Non-Specific Therapeutics For The Ailing Heart, Andrya Jean Durr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Morbidity and mortality of the diabetic population is influenced by many confounding factors, but cardiovascular disease (CVD), remains the leading cause of death. Mitochondrial dysfunction is central in the development of cardiac contractile dysfunction, with decreased mitochondrial bioenergetic function, increased dependence on free fatty acid utilization, and a decrease in glucose utilization having been shown to contribute to contractile dysfunction. Strategies targeting the amelioration of mitochondrial bioenergetic function are attractive for limiting diabetes-induced heart failure, and preserving health-span. The goals of this dissertation were to assess two mitochondrial-centric approaches for the amelioration of mitochondrial and cardiac contractile dysfunction in diabetes …

Modeling, Fabrication, And Characterization Of Rf-Based Passive Wireless Sensors Composed Of Refractory Semiconducting Ceramics For High Temperature Applications, Kavin Sivaneri Varadharajan Idhaiam

Graduate Theses, Dissertations, and Problem Reports (ETD)

Real-time health monitoring of high temperature systems (>500^oC) in harsh environments is necessary to prevent catastrophic events caused by structural failures, varying pressure, and chemical reactions. Conventional solid-state temperature sensors such as resistance temperature detectors (RTDs) and thermocouples are restricted by their operating environments, sensor dimensions and often require external power sources for their operation. The current work presents the research and development of RF-based passive wireless sensing technology targeting high temperatures and harsh environmental conditions. Passive wireless devices are generally classified as near-field and far-field devices based on the interrogation distance. Near-field sensors are placed at …

Combinatorial Approaches For Effective Design, Synthesis, And Optimization Of Enzyme-Based Conjugates, Jordan Scott Chapman

Graduate Theses, Dissertations, and Problem Reports (ETD)

The specificity and efficiency with which enzymes catalyze selective chemical reactions far exceeds the performance of traditional heterogeneous catalysts that are predominant in industrial applications such as conversion of commodity chemicals to value-added products, fuel cells, and petroleum refinement. Moreover, biocatalysts exhibit exceptionally high product turnover at ambient conditions with little health and environmental burden. These advantageous qualities have led to the prolific use of enzyme catalysis in pharmaceutical, detergents, and food preservation industries wherein their use has greatly reduced waste generation, Unfortunately, the full slate of benefits that enzymes can impart to a broader range of chemical processes is …

Playing-Related Medical Injuries And Health Conditions In Collegiate Saxophonists: A Survey Of Saxophonists In North American Universities, Michael Anne Tolan

Graduate Theses, Dissertations, and Problem Reports (ETD)

When compared to other areas of musical performance, medical research on the performing arts is limited when it comes to studies on woodwind players, particularly saxophonists. The gap in research may be attributed to the circumstances surrounding selection processes and identifying subjects for study. For example, saxophonists are not salaried professionals in orchestras, which are commonly pooled for medical research. Establishing contact with saxophonists to conduct studies can prove difficult due to the wide range of genres that utilize the saxophone. Some issues may also be attributed to research methodology. To find the onset of injuries, researchers in previous studies …

Syntheses Of Indoles And Natural Indole Alkaloids From 2-Nitrostyrene Derivatives, S M Ashikur Rahman

Graduate Theses, Dissertations, and Problem Reports (ETD)

A short and flexible synthesis of 2,3-disubstituted indoles employing a Barluenga-type palladium-catalyzed cross-coupling of p-tosylhydrazones with 2-nitroarylhalides followed by a palladium-catalyzed, carbon monoxide-mediated reductive cyclization was developed. Significantly better overall yields were obtained performing the two-step reaction sequence in a one-pot reaction. Secondly, in order to facilitate biological studies of ergotryptamine, a recently isolated ergot alkaloid, it was selected as a target for synthesis. In an attempt to synthesize ergotryptamine, a palladium catalyzed Kosugi-Migita-Stille cross coupling, a Mizoroki-Heck reaction, and a carbon monoxide-mediated palladium-catalyzed reductive cyclization were utilized as the key steps. Using a similar synthetic approach, synthesis of …

Analysis Of Emissions Profiles Of Hydraulic Fracturing Engine Technologies, Nicholas Joseph Wells

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the past twenty years natural gas production in the United States has significantly increased. This is largely due to technological advancements in unconventional methods of horizontal drilling and hydraulic fracturing. With these new and improved technologies, the United States has been able to increase its natural gas dry production per year by 14.5 trillion cubic feet per year from 2001-2021, a 74.1% increase. Horizontal drilling allows the wellbore to have more contact with the source rock, thus allowing more hydrocarbons to be extracted. Hydraulic fracturing allows oil and gas companies to have access to low permeable source rock, previously …

Post-Processing Precise Point Positioning Solutions With Parameter Optimization, Maria A. Gonzalez Castaneda

Graduate Theses, Dissertations, and Problem Reports (ETD)

Precise Point Positioning (PPP) technique can offer position solutions with centimeter-level accuracy by fusing precise satellite orbits and clocks with un-differenced, dual-frequency, pseudo-range, and carrier-phase observables. PPP presents a compelling alternative to Differential Global Positioning Systems, with the benefit that it only requires a single receiver and does not require simultaneous observations from many stations, making it appealing for ongoing research on hydro-graphic survey applications. The National Oceanic and Atmospheric Administration has been working on a buoy system tracked with a Global Positioning Systems receiver and Inertial Measurement Unit sensor using the PPP technique. In the interest of obtaining accurate …

Bone Marrow Stroma-Induced Transcriptome Signatures Of Multiple Myeloma As Modulated By Junb, Jasleen Kaur Gandhi

Graduate Theses, Dissertations, and Problem Reports (ETD)

The bone marrow (BM) microenvironment acts as a breeding ground for drug resistance in multiple myeloma (MM). The interaction with bone marrow stromal cells (BMSCs) confer environment-mediated drug resistance (EMDR) to multiple myeloma. We investigated BM stroma-induced transcriptome signatures of MM cells through a sophisticated analysis of gene expression. In particular, we defined transcription program modulated by JunB, an emerging regulator of MM pathogenesis and a member of the transcription factor superfamily activator protein 1 (AP-1), in response to BM stimulation. The data and results lay down a foundation for future studies to illustrate the regulatory role of JunB in …

Genetic Analysis And Telemetry Study Of Migration Habits Of The Endangered Atlantic Sturgeon, Cassia Marie Busch

Graduate Theses, Dissertations, and Problem Reports (ETD)

Atlantic sturgeon are a long-lived anadromous fish that ranged from Labrador, Canada to Florida, US. Due to overharvest in the late 1800’s and 1900’s, Atlantic sturgeon populations across the coast experienced a dramatic population crash. Recovery of this species has faced challenges due to anthropogenic threats, such as vessel strikes, bycatch, and habitat degradation. In 2012, Atlantic sturgeon were added to the United States Endangered Species Act (ESA). Under the ESA, populations were listed as five distinct population segments (DPS), reflecting their geographic arrangement and genetic structure: Gulf of Maine DPS (threatened), New York Bight DPS (endangered), Chesapeake DPS (endangered), …

A Domain Adaptation Approach For Segmenting Cell Instances In Microscopy Data, Matthew R. Keaton

Graduate Theses, Dissertations, and Problem Reports (ETD)

Automated cellular instance segmentation is a process that has been utilized for accelerating biological research since before the deep learning era, and recent advancements have produced higher quality results with less effort from the biologist. Most current endeavors focus on completely cutting the researcher out of the picture by generating highly generalized models. However, these models invariably fail when faced with novel data and effectively opt to miss out on the full capabilities of deep learning in pursuit of this goal. In our work, we demonstrate how, with even a minimal amount of annotated data, dominant approaches in this space …

Face Recognition With Attention Mechanisms, Qiangchang Wang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Face recognition has been widely used in people’s daily lives due to its contactless process and high accuracy. Existing works can be divided into two categories: global and local approaches. The mainstream global approaches usually extract features on whole faces. However, global faces tend to suffer from dramatic appearance changes under the scenarios of large pose variations, heavy occlusions, and so on. On the other hand, since some local patches may remain similar, they can play an important role in such scenarios. Existing local approaches mainly rely on cropping local patches around facial landmarks and then extracting corresponding local representations. …

Advancing Compound Detection And Identification Using Vibrating Sharp-Edge Spray Ionization, Anthony Paul Debastiani

Graduate Theses, Dissertations, and Problem Reports (ETD)

Advancing Compound Detection and Identification Using Vibrating Sharp-Edge Spray Ionization

Anthony Paul DeBastiani

‘Omics consists of various areas, including genomics, proteomics, lipidomics, and metabolomics with a goal of detection and identification of biomolecules present within each of these classes within an organism. Current omic analytical methods include the use of mass spectrometry (MS) coupled with various separation techniques, including liquid chromatography (LC), with the use of heated nebulization gas assisted electrospray ionization (HESI). Although the rapid progress in LC-MS platform development has lead to significant enhancements in omic studies, significant limitations in the detection and identification of low abundant species …

“I Lost Everything I Owned… Now I’M Growing That Back”: Narratives Of Redemption After Meth Immersed Lives, Danielle M. Stoneberg

Graduate Theses, Dissertations, and Problem Reports (ETD)

There is a lack of knowledge on how the process of desisting (i.e., maintaining abstinence) works for those leaving immersed drug lifestyles, leaving a need for a better understanding of the role and impact of identity in drug desistance. Through secondary analysis of interview data from a sample of 33 former methamphetamine manufacturers, this study examines the applicability of Maruna’s (2001) redemption narrative framework and identifies other aspects featured in their narratives that signaled identity change. Results found all participants discussed at least one component of the framework in their interviews. Their narrative identities changed over time to recognize that …

Utilizing The K18-Hace2 Mouse Model To Develop Protective Covid-19 Vaccines, Ting Y. Wong

Graduate Theses, Dissertations, and Problem Reports (ETD)

The ongoing Coronavirus Disease 2019 (COVID-19) pandemic is caused by the respiratory virus Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Similar to other respiratory viruses, SARS-CoV-2 is transmitted through inhalation of respiratory droplets and aerosols from infected individuals. Once inhaled, SARS-CoV-2 utilizes the receptor binding domain (RBD) on the spike protein to bind to human Angiotensin Converting Enzyme 2 (hACE2) receptor to gain entrance into host cells to begin viral replication. SARS-CoV-2 infection can result in mild to severe cases of COVID-19 ranging from asymptomatic infections, cold or flu like symptoms to respiratory failure. The onset of the pandemic in …

Examining Evolutionary Rate In Xiphosura, Samantha B. Ocon

Graduate Theses, Dissertations, and Problem Reports (ETD)

Horseshoe crabs, a group of aquatic chelicerate arthropods of the class Xiphosura, are strongly linked with the concept of “living fossils” – a term colloquially used to refer to clades that display a consistently low rate of morphological evolution through time. The concept of living fossils has been hotly debated, as it is considered to simplify or obscure millennia of evolutionary change. Recent methodological and computational advances in the paleobiological sciences have allowed for the investigation of these claims. Xiphosura are a model taxon for this type of investigative study because they exhibit a complex evolutionary history, despite their reputation …

Examining The Relationship Among Perceived Academic Climate, Belongingness, And Engineering Identity, Sumaia Ali Raisa

Graduate Theses, Dissertations, and Problem Reports (ETD)

An attempt to cultivate an inclusive learning environment in engineering is trending as a response to women's underrepresentation and a lower retention rate than men undergraduates. This study was situated in such an undergraduate engineering program where interventions were embedded in the course curriculum focusing on cultivating an inclusive engineering identity. Following a sociocultural perspective, the present study aimed to examine the relation of engineering identity with perceived academic climate, sense of belonging, and gender among two engineering cohorts (before covid and during covid context). A total of 482 first-year engineering undergraduates' survey responses were analyzed in this study using …

Transitions Between The Steps Of Group Iic Intron Forward And Reverse Splicing And Integration Into Dna, Claire Mae Smathers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Group II introns are ancient ribozymes capable of self-excision and mobility into new genomic locations. As the ancestors of both the eukaryotic spliceosome and eukaryotic retroelements, these simple RNA machines serve as excellent models for understanding the mechanisms of both gene splicing and retroelement activity in eukaryotes. Although group II introns have been studied for decades, knowledge of the most unique and arguably evolutionarily relevant class of group II introns, IIC, is limited. Our knowledge is especially limited in terms of understanding how IIC introns mediate the transition between the two transesterification steps of forward splicing and reverse splicing into …

Targeting Vulnerabilities In Cell State And Calcium Signaling For The Treatment Of Lung Cancer, Clark Jones

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lung cancer remains the deadliest of all cancers due to the high mutational burden associated with the disease. Combating mutational drivers and drug resistance proves to be essential in the development of novel therapies to improve patient outcomes. A first-in-class cyclic peptide known as MTI-101 has been shown to induce necrotic cell death in a caspase independent manner. MTI-101 was derived from the linear peptide known as HYD1 that was found in a high throughput screen to block cell adhesion with the extracellular matrix. The compound was further optimized and cyclized to the currently used MTI-101 that was found to …

Phenomic And Genetic Controls Of The Drought Stress Response In Sorghum, Melissa A. Lehrer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Drought, one of the most common abiotic stressors, is a result of the precipitation and temperature fluctuations influenced by climate change. As consistent weather patterns are crucial for the maintenance of crop yield, drought threatens food security through its impact on plant growth and development. It is essential to ensure the quality, availability, and affordability of grain-based products in the face of climate change due to expectations of population growth. Therefore, shedding light on the mechanisms associated with drought tolerance is integral to maintaining agricultural production under water-limited conditions. My dissertation work aimed to uncover the morphological, physiological, and genetic …

Impact Of Haemonchus Contortus On Feed Efficiency And Feeding Behavior Of Katahdin And Suffolk Lambs, Camren L. Maierle

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nutritional impact of internal parasitism, specifically Haemonchus contortus (Hc), is a significant contributor to production losses in small ruminants. Both chronic and subclinical infections generate millions of dollars of losses worldwide. Understanding the nutritional impact of Hc on lamb performance is crucial in determining the additional nutrient requirements of infected individuals. The aim of this study was to evaluate nutritional impact of Hc on infected Katahdin lambs with extensive genetic selection for parasite resistance and susceptible Suffolk lambs. Growth, feeding behavior, and feed efficiency (RFI. F:G) were evaluated during artificial Hc infection. Katahdin ewes from the Virginia …

Small Mammal Communities Of Restored And Natural Wetlands In West Virginia, Krista Noe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Wetland restoration is commonly practiced as part of conservation programs or wetland mitigation, which attempts to offset human-created losses of natural wetlands. However, because of the intrinsic and human-derived value of wetlands, it is critical to determine whether these wetlands truly act similarly to natural wetlands. One role of wetlands is to provide habitat for a diverse array of wildlife species. Small mammals are often overlooked taxa in wetland restoration efforts. However, they are essential to the wetland system because they influence vegetation and are prey for higher trophic level wildlife. I discuss considerations of restored wetlands, wildlife responses to …

Investigating The Mitochondrial Protein Mitoneet In C. Elegans Models Of Aging And Alzheimer's Disease, Jacob Ryan Boos

Graduate Theses, Dissertations, and Problem Reports (ETD)

Oxidative stress is an imbalance between reactive oxygen species production and elimination, favoring the former. Reactive oxygen species serve as important signaling molecules for physiological homeostasis. However, when produced in excess, these once important signaling molecules become detrimental, disrupting cellular functions, and ultimately leading to cell death. In aging, reactive oxygen species production is increased, accompanied with reductions in oxidative stress resistance, increasing the risk for developing age-related diseases including cardiovascular disease, cancer, stroke, and neurodegenerative diseases. The outer mitochondrial membrane iron-sulfur cluster containing protein mitoNEET (CISD1; gene CISD1) has shown to be a mediator of mitochondrial function and …