Digital Commons Network^™

Nutritional Status In Critical Care Of Covid-19 Patients, Dolph Lewis Davis Iii

Theses and Dissertations--Nutrition and Food Systems

Critical care nutrition is a poorly researched, and such a sudden pandemic event requiring manipulation of nutritional status via propofol-induced sedation for mechanical ventilation is potentially impactful on patient outcomes. This paper seeks to provide context to the impact of critical care protocols on the nutritional status of obese patients suffering from COVID-19.

The Role Of Nitric Oxide In Inter- And Intra- Cellular Signaling In Plant Defense, Fan Xia

Theses and Dissertations--Plant Pathology

Plants have evolved a sophisticated immune system to defend themselves against pathogens. This immune response can be triggered in response to pathogen-associated molecular patterns (PAMP) or specialized effectors that are recognized by the plant resistance (R) proteins. The latter, commonly referred to as effector-triggered immunity (ETI), is well known to induce broad-spectrum resistance throughout the plants. This phenomenon known as systemic acquired resistance (SAR) is regulated by several chemical signals including salicylic acid (SA), and free radical nitric oxide (NO) and reactive oxygen species (ROS). These signals operate in two parallel branches with NO/ROS functioning downstream of pipecolic acid (Pip) …

An Examination Of Organic Options In Tomato Systems And Their Use As Alternatives To Copper-Based Products, Erica Ann Fealko

Theses and Dissertations--Plant Pathology

Organic farming is an ever-increasing segment of tomato production. Currently, limited information is available which directly compares conventional to organic treatment programs for disease control in tomato production. Furthermore, many methods available rely on the use of copper products which may be contribute to high Cu levels in agricultural soils. In this study, the efficacies of current conventional and organic methods were compared. In addition, newer disease-control programs, with and without copper were examined, which potentially could reduce over-reliance on copper products.

Standard organic and conventional spray programs were conducted over a four year period targeting two pathogens, Alternaria tomatophila …

Identifying Bisphosphonate Protein Biomarkers In Equine Sera Using Mass Spectrometry Methods, Malinda Jayne Alison Porter

Theses and Dissertations--Veterinary Science

Bone resorptive diseases affect humans and horses, alike. Examples of these diseases include osteoporosis, Paget’s disease, and osteogenesis imperfecta in humans, and navicular disease in the horse (Mitchell et al., 2019; Suva et al., 2021). Bisphosphonates are used to treat these diseases, and may remain in the bone for several years after administration, demonstrating the need for newer drug testing methods.

The purpose of the discovery phase of this study was to use blood samples of in-training horses which had been given an initial administration of the bisphosphonate tiludronate and identify protein biomarkers that changed in response to this class …

The Effect Of Intra-Articular Corticosteroids On The Systemic Messenger Rna Response In An Equine Experimental Inflammation Model, Emma Elaine Partridge

Theses and Dissertations--Veterinary Science

Intra-articular (IA) corticosteroids are regularly used in equine athletes for the control of joint inflammation. The goal of this study was to utilize an acute synovitis inflammation model to determine the residual effects of IA betamethasone and triamcinolone acetonide on various inflammatory parameters and lameness.

Five mixed-breed, 2-year-old horses were randomly allocated to an IA treatment of the radiocarpal joint with 9 mg of either betamethasone or triamcinolone acetonide. Two weeks following treatment, horses were injected with 1μg of lipopolysaccharide (LPS) diluted in 1 mL of 0.9% sterile saline. Following LPS injection, horses were crossed-over and both sets of injections …

Dissecting The Role Of Actin-Myosin Motor And Calcium – Based Organelle Secretion In Motility Of Sarcocystis Neurona, Parul Suri

Theses and Dissertations--Veterinary Science

Sarcocystis neurona is a protozoan parasite that causes a rare neurological disorder in horses called Equine Protozoal Myeloencephalitis (EPM). Apicomplexa use actin-myosin based motor and organelle secretion to interact with the host cell and invade it. Despite the importance of motility and invasion-needed factors, the mechanisms by which S. neurona employs host cell association strategies to interact remains largely undefined. To address this knowledge gap, we hypothesize that just like other Apicomplexa, S. neurona utilizes actin polymerization for substrate-dependent gliding. Moreover, we also hypothesize that micronemes of S. neurona secrete proteins that are calcium-dependent. Based on previous studies in T. …

The Effects Of Cannabidiol On Senior Equine Health, Shelley Turner

Theses and Dissertations--Veterinary Science

Cannabis sativa L., has been revealed to produce hundreds of phytocannabinoids, of which cannabidiol (CBD) is one of the most desired. It has been revealed that CBD can potentially treat inflammation and act as an analgesic in humans without psychoactive effects of delta-9-tetrahydrocannabinol (THC). Recently, there has been interest in understanding the potential health benefits of CBD for horses. With an increasing senior horse population (< 15 years old), alternatives to the use of non-steroidal anti-inflammatory drugs (NSAIDs) such as phenylbutazone, firocoxib, and flunixin meglumine, are desired as these common anti-inflammatory treatments have negative side effects. Because senior horses may have chronic low-grade inflammation, termed inflamm-aging, they are a natural model to investigate the efficacy of CBD on inflammatory responses as well as other health parameters. Given this interest, and due to the lack of research conducted on CBD in the horse, the overall objective of this dissertation work was to conduct both basic and applied research investigating the effects of CBD on equine immune function and health. The first objective was to determine the in vitro effects of CBD as an anti-inflammatory. Peripheral blood mononuclear cells (PBMCs) from senior horses were cultured in vitro with increasing concentrations of pure CBD dissolved in dimethyl sulfoxide (DMSO). These cells were …

The Microbiome Of The Equine Roundworm, Parascaris Spp., Jennifer Lynn Cain

Theses and Dissertations--Veterinary Science

Parasitic nematodes, including the large roundworms colloquially known as ascarids, affect the health and well-being of livestock animals worldwide. The equine ascarid, Parascaris spp., was the first ascarid parasite to develop wide-spread anthelmintic drug resistance, with other species slowly following suit. There are no new classes of anthelmintics currently in development, and a solution to the ever-increasing prevalence of resistance is desperately needed. The microbiome has been shown to be an important factor in the fitness and health of many organisms and changes to microbiome composition have been associated with a plethora of diseases. The microbiome is also important to …

Evaluating Restoration Outcomes: Red Spruce Reforestation In The West Virginia Highlands, Benjamin Rhodes

Theses and Dissertations--Forestry and Natural Resources

Red spruce (Picea rubens) is the keystone species of the red spruce-northern hardwood forest, a unique high-elevation plant community that supports hundreds of animal species in the Central Appalachians. These forests were devastated by the Industrial Logging Era of the late 1800s and early 1900s, and they remain in a degraded and fragmented state. Current restoration efforts include red spruce plantings on old field sites and reclaimed coal mines. This project seeks to aid those efforts by evaluating restoration outcomes for vegetation and soils along a ten-year chronosequence. Specifically, the study aims to determine whether restoration site soils …

Spatial Ecology Of Rocky Mountain Elk (Cervus Canadensis Nelsoni) Cows In Southeastern Kentucky, Nathan Dennis Hooven

Theses and Dissertations--Forestry and Natural Resources

The elk (Cervus canadensis) was extirpated from its range in eastern North America by the end of the 1800s, prompting several U.S. states and Canadian provinces to begin translocation programs with the goal of reestablishing elk populations. While eastern elk managers have relied on information from western herds to guide population and habitat management, there is a need for region-specific research on the spatial ecology and habitat associations of translocated elk given the stark differences in landscape, climate, predator communities, and harvest regimes across the continent. While the Kentucky elk reintroduction is one of the best documented programs …

The Response Of Beef Cattle To Disturbances From Unmanned Aerial Vehicles (Uavs), Gabriel Abdulai

Theses and Dissertations--Biosystems and Agricultural Engineering

Unmanned aerial vehicles (UAVs) are increasingly becoming common in animal agriculture. However, research regarding the impact of UAV disturbance on animal wellbeing is lacking or limited. The goal of this study was to investigate the effect of UAV flights on beef cattle by measuring cattle heart and movement rate when introduced to single or multiple UAV flights. A total of 16 -18 crossbred beef heifers were introduced to different flights patterns at between 5 and 9 m above ground level (AGL) at approximately 1 to 2 m/s horizontal velocity for 4 weeks with flights repeated 3 days per week. Results …

Lignin Valorization Via Reductive Depolymerization Using Promoted Nickel Catalysts And Sub- And Supercritical Methanol, Julia Parker

Theses and Dissertations--Biosystems and Agricultural Engineering

While lignin has been regarded as the most promising renewable feedstock for the sustainable manufacture of aromatic compounds, lignin valorization is necessary to improve the economic viability of biorefineries. Reductive catalytic fractionation (RCF), which combines delignification and lignin depolymerization into a single stage while maintaining the structure and integrity of the cellulose component, has evolved as an effective method for processing biomass. The ability of Cu and Fe to promote the performance of a 20% Ni/alumina catalyst when converting native lignin to alkylphenols by RCF in sub- and supercritical methanol was tested. The effectiveness of lignin extraction as measured by …

Sediment Nitrogen Dynamics In Backwater Wetland Confluences Of A Regulated River, Gina Degraves

Theses and Dissertations--Biosystems and Agricultural Engineering

As harmful algal blooms in regulated river systems have increased in the past decade, the importance of understanding sediment nutrients has also increased. Research linking nutrient processes and fine sediment dynamics to harmful algal blooms in confluence wetlands along regulated rivers has recently become apparent. However, the relationship between sediment nutrient dynamics in confluence wetlands has been understudied. Utilization of sediment fingerprinting, high-frequency water quality monitoring, and tracer unmixing mass-balance modeling, was able to suggest sediment N mineralization in Appalachia confluence riparian wetland was not a dominate source of nitrate downstream. Further measures of supplementary tracers and additional sediment sources …

Classifying And Mapping Aquatic Vegetation In Heterogeneous Stream Ecosystems Using Visible And Multispectral Uav Imagery, Rozalia Agioutanti

Theses and Dissertations--Biosystems and Agricultural Engineering

The need for assessment and management of aquatic vegetation in stream ecosystems is recognized given the importance in impacting water quality, hydrodynamics, and aquatic biota. However, existing approaches to monitor are laborious and its currently not feasible to track spatial and temporal differences at broad scales. The objective of this study was therefore to map and classify aquatic vegetation of a shallow stream with heterogenous mixtures of emergent and submerged aquatic vegetation. Data was collected in the Camden Creek watershed within the Inner Bluegrass Region of central Kentucky. The use of unmanned aerial vehicles (UAVs) was employed and both visible …

Exploration Of Lignin-Based Superabsorbent Polymers (Hydrogels) For Soil Water Management And As A Carrier For Delivering Rhizobium Spp., Toby Adjuik

Theses and Dissertations--Biosystems and Agricultural Engineering

Superabsorbent polymers (hydrogels) as soil amendments may improve soil hydraulic properties and act as carrier materials beneficial to soil microorganisms. Researchers have mostly explored synthetic hydrogels which may not be environmentally sustainable. This dissertation focused on the development and application of lignin-based hydrogels as sustainable soil amendments. This dissertation also explores the development of pedotransfer transfer functions (PTFs) for predicting saturated hydraulic conductivity using statistical and machine learning methods with a publicly available large data set. A lignin-based hydrogel was synthesized, and its impact on soil water retention was determined in silt loam and loamy fine sand soils. Hydrogel treatment …

Effects Of Physiological Stage And Diet On Drosophila Suzukii Olfaction, Aerianna Shindindowi Littler

Theses and Dissertations--Entomology

Massive economic damage by spotted-wing drosophila (SWD), Drosophila suzukii, results from their unique egg laying behavior. Gravid SWD flies employ a complex suite of behaviors to find fruit with the optimum firmness and chemistry. We tested for SWD attraction to various fruits and found overripe raspberries to be most attractive to gravid flies which was unexpected since they oviposit in ripe fruit. We next tested the same choice assay in a T-maze and found that the results were comparable. We also found discrete odor profiles among the ripening stages. When testing for sugars and CO2 concentrations from field-collections, overripe …

Net Effects Of Wild And Managed Birds In Agroecosystems, Karina Garcia

Theses and Dissertations--Entomology

Agricultural intensification and expansion have reduced biodiversity in agroecosystems, jeopardizing the ecosystem services that nature provides to humans in these landscapes including wildlife-mediated pest control. Among these purveyors of ecosystem services are birds, who can provide services to agroecosystems by consuming arthropod pests. Yet some bird species also act as pests by consuming crops. Herein, I use molecular diet analysis through high-throughput sequencing on DNA extracted from bird fecal samples to examine birds’ diet and classify bird species as consumers of major strawberry pests (Lygus spp.). Additionally, I use targeted PCR approaches to determine which bird species consumed strawberries. …

Midgut Metamorphosis In The Yellow Fever Mosquito, Aedes Aegypti, Najla Mohammed Albishi

Theses and Dissertations--Entomology

Remodeling of larval tissues including the midgut is essential for the completion of metamorphosis in the yellow fever mosquito, Aedes aegypti (Diptera: Culicidae). The midgut structure and function have been extensively studied in adult Ae. aegypti, because adult females need to blood feed to reproduce, and the alimentary canal is the first barrier that encounters pathogens ingested through the blood meal. The midgut of Ae. aegypti undergoes remodeling during the larval-pupal metamorphosis. The degeneration of larval cells and the proliferation and differentiation of stem cells to form pupal-adult midgut are the major events that take place during metamorphosis. …

Rna Interference For Emerald Ash Borer Suppression: Ecotoxicological Assessment And Delivery Methods, Flavia Pampolini

Theses and Dissertations--Entomology

The emerald ash borer (EAB), Agrilus planipennis (Coleoptera: Buprestidae), is a highly invasive phloem-feeding insect native to Asia. It has killed millions of ash trees (Fraxinus spp.) since its accidental introduction into North America, causing profound economic and ecological impacts. RNA interference (RNAi) or double-stranded RNA (dsRNA)-mediated gene silencing is an emerging biotechnology that is developing as an innovative tool for pest management. Exogenous dsRNA triggers the RNAi pathway, silences genes, and disrupts protein function, causing insect mortality. RNAi has proven effective in reducing target gene expression and causing mortality in EAB; however, two aspects stand as barriers …

Gene Silencing Provides Insights Into Bark Beetle Biology And Creates Potential For Broad Scale Forest Protection, Beth R. Kyre

Theses and Dissertations--Entomology

Dendroctonus bark beetles are among the most economically and ecologically significant forest pests in North America and play a critical role in the overall health of conifer forest ecosystems. Dendroctonus bark beetles influence ecosystem benefits and biodiversity and drive forest succession, and adversely affect timber production, forest management, and recreation. As temperatures surge and climatic fluctuations become more extreme, catastrophic bark beetle outbreaks are increasing in frequency, escalating pressures on highly vulnerable conifer forests already compromised by heat and drought. Eruptive outbreaks of Dendroctonus beetles are largely unhindered by traditional silvicultural management; these practices further disrupt forest ecosystem services, including …

A Functional Genomics Approach To Overwintering Mechanisms In Insects, Emily Allison Wheeler Nadeau

Theses and Dissertations--Entomology

Insects living in temperate and polar environments have developed numerous adaptations to increase survival at low temperatures. A majority of insects are freeze-intolerant and die from internal ice formation, but some are freeze-tolerant and can survive ice formation in extracellular spaces. Both categories of insects prepare for winter with a combination of seasonal and rapid acclimation responses, which differ both in time course and in underlying mechanisms. In this dissertation, I examine adaptations for winter survival in several insect species with a specific focus on molecular mechanisms. To better understand the underpinning mechanisms of these adaptations, I leverage functional genomics …

The Influence Of Sociopolitical Factors On Adolescents’ And Youths’ Development, Banafsheh Aghayeeabianeh

Theses and Dissertations--Family Sciences

Youths and adolescents are one of the major perpetrators of antisocial and deviant behaviors, which have deleterious consequences for both the perpetrators and society. Although there is extensive literature on youth and adolescent antisocial behavior, some correlates of youth antisociality are not known yet. As such, the present study is devoted to understanding the micro- and macro-level predictors of youth and adolescents’ antisociality in three contexts. Three empirical studies applying bioecological systems theory and analyzing data from the International Dating Violence database using Mixed Effects Models were conducted to investigate the ecology of the development of antisocial behaviors among youth. …

Associations Of Sexual Satisfaction, Relationship Quality, And Cognitive Functioning Within Intimate Relationships In Older Adulthood, Allison G. Smith

Theses and Dissertations--Family Sciences

The roles of sexual satisfaction, relationship quality, and cognitive functioning within older adults (N = 538) intimate relationships are assessed at two timepoints over an 11-year timeframe. Longitudinal data from 63 older adults are assessed through a path modeling approach to examine how sexual satisfaction and relationship quality change throughout older adulthood. Cross-sectional data for 538 older adults are utilized to examine the association between cognitive health and sexual satisfaction/relationship quality. Qualitative data from 125 older adults are utilized to understand how older adults describe their intimate relationships. Results from path models demonstrate that one’s degree of sexual satisfaction …

#Askrenal: Use Of An Automated Twitter Account To Crowdsource Nephrology Queries, Jade M. Teakell, Alicja Rosolowska, Swapnil Hiremath, Matthew A. Sparks, Edgar V. Lerma, Joel M. Topf, Nikhil A. Shah

Conference Presentation Abstracts

Background: Social media platforms are used in contemporary crowdsourcing, and Twitter is apt for reaching a large number of people with a common interest. Users, especially those with a small follower count may find it challenging to reach a large audience. #AskRenal was developed as a Twitter crowdsourcing tool to help users get answers to nephrology questions. We hypothesized that the #AskRenal hashtag could be used by anyone to receive helpful and timely responses to simple or complex nephrology questions posed on the social media platform. Methods: A Twitter account @AskRenal, and an online Twitter bot that automatically retweeted any …

Writing Arguments In Stem, Jason Peters, Jennifer Bates, Erin Martin-Elston, Sadie Johann, Rebekah Maples, Anne Regan, Morgan White

OER Course Materials

A team of faculty at California Polytechnic State University, San Luis Obispo, curated the contents to support instructors teaching first-year courses in critical thinking and communication.

Alice In The Land Of Artificial Intelligence: Some Practical Ways To Help Survive “Abstract Idea” Challenges Under Section 101, Charlene Thrower

Student Works

No abstract provided.

Product Liability Applied To Automated Decisions, Ian A. Wardell

Student Works

No abstract provided.

Pharmacy Benefit Managers: What Are The Real Costs Behind-The-Counter?, Gabrielle Landes

Student Works

No abstract provided.

How New Jersey Can Mitigate Climate Change On Its Coasts Through Nuisance-Based Restrictions On Development, Jennifer Garster

Student Works

No abstract provided.

Ai & Arms Control: The Cold War Continued, Robert Sobon

Student Works

No abstract provided.