Digital Commons Network^™

Seasonal Changes In Mitochondrial Bioenergetics And Physiological Performance Of The Bluegill Sunfish, Lepomis Macrochirus, From A Shallow, Midwest River., Derick I. Lamptey, Ryan W. Sparks, Rolando Monte De Oca, Robert Skolik, Michael Menze, Eloy Martinez

Faculty and Staff Scholarship

As global temperature shifts due to anthropogenic impacts, the frequency and magnitude of extreme temperatures in shallow aquatic ecosystems are expected to increase. Previous studies performed on fish under fluctuating temperature regimes have reported increased physiological stresses, compared to stable thermal conditions, leading to morphological, behavioral and biochemical adaptations. Moreover, many studies have reported the effects of warmer temperatures on numerous aquatic species, but seasonal temperature changes experienced in aquatic systems are understudied. We surveyed changes in the bioenergetic machinery of a native fish population ( Lepomis macrochirus) in the temperate zone of North America and observed season-dependent thresholds in …

Radical-Initiated Brown Carbon Formation In Sunlit Carbonyl–Amine–Ammonium Sulfate Mixtures And Aqueous Aerosol Particles, Natalie G. Jimenez, Kyle D. Sharp, Tobin Gramyk, Duncan Z. Ugland, Matthew-Khoa Tran, Antonio Rojas, Michael A. Rafla, Devoun Stewart, Melissa M. Galloway, Peng Lin, Alexander Laskin, Mathieu Cazaunau, Edouard Pangui, Jean-François Doussin, David O. De Haan

Chemistry and Biochemistry: Faculty Scholarship

Brown carbon (BrC) formed from glyoxal+ammonium sulfate (AS) and methylglyoxal+AS reactions photobleaches quickly, leading to the assumption that BrC formed overnight by Maillard reactions will be rapidly destroyed at sunrise. Here, we tested this assumption by reacting glyoxal, methylglyoxal, glycolaldehyde, or hydroxyacetone in aqueous mixtures with reduced nitrogen species at pH 4–5 in the dark and in sunlight (>350 nm) for at least 10 h. The absorption of fresh carbonyl+AS mixtures decreased when exposed to sunlight, and no BrC formed, as expected from previous work. However, the addition of amines (either methylamine or glycine) allowed BrC to form in …

In Situ Structure Of The Acrab-Tolc Efflux Pump At Subnanometer Resolution, Muyuan Chen, Xiaodong Shi, Zhili Yu, Guizhen Fan, Irina I Serysheva, Matthew L Baker, Ben F Luisi, Steven J Ludtke, Zhao Wang

Faculty, Staff and Students Publications

The tripartite AcrAB-TolC assembly, which spans both the inner and outer membranes in Gram-negative bacteria, is an efflux pump that contributes to multidrug resistance. Here, we present the in situ structure of full-length Escherichia coli AcrAB-TolC determined at 7 Å resolution by electron cryo-tomography. The TolC channel penetrates the outer membrane bilayer through to the outer leaflet and exhibits two different configurations that differ by a 60° rotation relative to the AcrB position in the pump assembly. AcrA protomers interact directly with the inner membrane and with AcrB via an interface located in proximity to the AcrB ligand-binding pocket. Our …

Covid-19 Pandemic Impact On Psychotropic Prescribing For Adults With Intellectual Disability: An Observational Study In English Specialist Community Services, Danial Naqvi, Bhathika Perera, Sarah Mitchell, Rory Sheehan, Rohit Shankar

Peninsula Medical School

Background Coronavirus disease 2019 (COVID-19) has had a disproportionate impact on people with intellectual disability (PwID). PwID are at higher risk of mental illness and receive psychotropic prescribing 'off licence' also, to manage distress behaviour. The lockdown and reduction of multidisciplinary face-to-face appointments had an impact on care delivery, the recourse possibly being psychotropic prescribing. It is imperative to comprehend the influence the pandemic had on psychotropic prescribing patterns to enable future planning. Aims The aim was to understand the impact of the pandemic by comparing psychotropic prescribing patterns during the England lockdown with the prescribing patterns before lockdown in …

Constitutively Active Sarm1 Variants That Induce Neuropathy Are Enriched In Als Patients, A Joseph Bloom, Xianrong Mao, Amy Strickland, Yo Sasaki, Jeffrey Milbrandt, Aaron Diantonio

2020-Current year OA Pubs

BACKGROUND: In response to injury, neurons activate a program of organized axon self-destruction initiated by the NAD

METHODS: To investigate whether naturally occurring human variants might disrupt SARM1 autoinhibition and potentially contribute to risk for neurodegenerative disease, we assayed the enzymatic activity of all 42 rare SARM1 alleles identified among 8507 amyotrophic lateral sclerosis (ALS) patients and 9671 controls. We then intrathecally injected mice with virus expressing SARM1 constructs to test the capacity of an ALS-associated constitutively active SARM1 variant to promote neurodegeneration in vivo.

RESULTS: Twelve out of 42 SARM1 missense variants or small in-frame deletions assayed exhibit constitutive …

Place Is Power: Investing In Communities As A Systemic Leverage Point To Reduce Breast Cancer Disparities By Race, Matthew Jay Lyons, Senaida Fernandez Poole, Ross C Brownson, Rodney Lyn

2020-Current year OA Pubs

Racial disparities in breast cancer present a vexing and complex challenge for public health. A diverse array of factors contributes to disparities in breast cancer incidence and outcomes, and, thus far, efforts to improve racial equity have yielded mixed results. Systems theory offers a model that is well-suited to addressing complex issues. In particular, the concept of a systemic leverage point offers a clue that may assist researchers, policymakers, and interventionists in formulating innovative and comprehensive approaches to eliminating racial disparities in breast cancer. Naming systemic racism as a fundamental cause of disparities, we use systems theory to identify residential …

Prospects For Valuation In Marine Decision Making In Europe, Rob Tinch, Stephen Hynes, Claire Armstrong, Wenting Chen

Journal of Ocean and Coastal Economics

There is now high-level recognition that the UN Sustainable Development Goals can only be achieved if the decline of ecosystems and biodiversity can be halted and reversed. This will require effective control of ongoing pressures, meaningful protection and enforcement of protected areas, and significant investments in ecosystem restoration. This paper explores the possible use of economic valuation and appraisal in achieving these goals in marine systems and discusses the European marine policy instruments where they should have an important role The paper first briefly reviews the tools of economic valuation and appraisal for marine ecosystem management. A critique of the …

Blue Growth: A Transitions Approach To Developing Sustainable Pathways, Christina Kelly, Ben Mcateer, Frances Fahy, Liam Carr, Daniel Norton, Desiree Farrell, Rebecca Corless, Stephen Hynes, Zacharoula Kyriazi, Agnès Marhadour, Regis Kalaydjian, Wesley Flannery

Journal of Ocean and Coastal Economics

The sustainable management of Blue Growth is an urgent issue for coastal states. Marine industries have rapidly expanded over the last two decades and this is projected to continue with the European Green Deal and post-COVID economic recovery policies. The intensification of Blue Growth could have adverse socio-ecological implications and must, therefore, be managed in terms of sustainability, natural resource boundaries, and coastal community well-being. Managing Blue Growth in a sustainable manner however, is challenging due to the longstanding inefficiencies and inertia of existing marine governance regimes. Adopting a transitions approach has been advanced as a way of steering regime …

Challenges In Measuring Indicators Of Progress For The Atlantic Action Plan, Daniel Norton, Regis Kalaydjian, Stephen Hynes, Arantza Maza Murillas, Javier Fernandez-Macho, Wesley Flannery, Maria Pilar Gonzalez Casimiro, Agnès Marhadour, Zacharoula Kyriazi, Christina Kelly, Raul Prellezo, Marta Escapa, Erwann Quimbert, Noel Ballantyne, Rebecca Corless, Maria Pafi, Yang Yaqi

Journal of Ocean and Coastal Economics

The EU Atlantic Action Plan (AAP) has recently been updated and revised to support ‘blue growth’ along Europe’s western coastal regions. The revisions reflect recent challenges facing the Atlantic Arc maritime economies including the Covid-19 crisis, Brexit and the new requirements of the European Green Deal. This new revision, termed AAP 2.0, also addresses some of the weaknesses highlighted in the original Atlantic Action Plan particularly regarding identifying indicators that may be used to measure progress in the achievement of the Plan’s objectives. Using a database with comparable marine socio-economic data across the Atlantic regions, a number of indicators are …

Developing Ecosystem Accounts For The Marine And Coastal Environment: Limitations, Opportunities And Lessons Learned From The United Kingdom Experience., Gaetano Grilli, Tiziana Luisetti, Ann Thornton, Deanna Donovan

Journal of Ocean and Coastal Economics

The marine and coastal environment provides crucial ecosystem services to sustain human activities and wellbeing. Advancing measurement frameworks to record the contribution of marine and coastal ecosystems to national wealth and economies and assess the sustainability of their use is therefore paramount. The recently proposed Ocean Accounting Framework outlined within the System of Environmental Economic Accounting represents a decisive step in this direction and provides a foundation for developing an integrated and standardised set of ecosystem accounts to support decision making and use of the seas. However, the ecological and socio-economic complexities of marine and coastal systems pose several challenges …

Kentucky Alfalfa Conference: A Team Approach To Alfalfa Education And Technology Transfer, Garry D. Lacefield, Monroe Rasnake, Christi L. Forsythe, Jimmy C. Henning, Bill Talley

IGC Proceedings (1977-2023)

Alfalfa has played an important role in providing farmers of Kentucky with a high yielding, high quality, persistent forage legume for many years. In 1980, a group of individuals under the leadership of University of Kentucky Forage Extension Specialists organized and implemented the first statewide educational event on alfalfa. This initial meeting gave rise to the Kentucky Alfalfa Conference that celebrated it’s twentieth anniversary in February 2000. This annual event has been co-sponsored by the University of Kentucky, Kentucky Forage and Grassland Council, along with other state and national groups including Certified Alfalfa Seed Council, Kentucky Department of Agriculture, Kentucky …

Developing A National Alfalfa Information System, David B. Hannaway, K. J. Hannaway, P. Sohn, S. Griffith, B. E. Avery, E. Nowick, W. F. Wedin, L. R. Vough, S. C. Bosworth, Garry D. Lacefield, Gary E. Bates, D. Undersander, N. P. Martin, J. Caddel, G. L. Kilgore, S. B. Orloff, A. M. Gray, R. Ditterline, T. Griggs, D. H. Putnam, M. J. Ottman

IGC Proceedings (1977-2023)

Using state-of-the-art telecommunication technologies, this project is developing a comprehensive knowledge resource for alfalfa (Medicago sativa L.); the National Alfalfa Information System (NAIS). This project will serve as an improved model for Extension educational programs. Alfalfa is the most important forage crop in the USA and grown worldwide for feeding millions of livestock and in many cropping systems. As a legume, it is important in sustaining the environment and the productivity of agriculture. Information needs are present in every state and internationally. The NAIS is being developed through national and international cooperation, putting the best science-based alfalfa information and …

Humboldt In The Time Of Covid : Youth's Mental Health With Paula Nedelcoff Lmft, Noe Sanchez-Nino, Paula Nedlecoff

Humboldt in the Time of COVID - Digital Archive

Our interview conducted on December 6, 2021 at 10:00 AM, took place over zoom. Although both participants Audio was recorded on a separate device. We discuss mental health in general, how local students have adapted to the change, and the challenge students and service providers have encountered in offering therapy.

Humboldt In The Time Of Covid : Available Substance Abuse Support With Reana West, Noe Sanchez-Nino, Reana West

Humboldt in the Time of COVID - Digital Archive

Our interview conducted on December 12, 2021 at 3:40 PM, took place over zoom. Audio was recorded on a separate device. We discuss access to substance abuse disorder in the time of Covid and how it has evolved to meet the needs of vulnerable citizens.

Celebrations 2022, University Of Mississippi Writing Project, University Of Mississippi. Office Of Pre-College Programs.

Celebrations: A Literary Magazine

No abstract provided.

January 5, 2022 Greenmail, University Of Alabama At Birmingham

GreenMail (student newsletter through 2024)

No abstract provided.

Comparison Of Percutaneous Vs Oral Infection Of Hamsters With The Hookworm Ancylostoma Ceylanicum: Parasite Development, Pathology And Primary Immune Response, Richard D. Bungiro, Lisa M. Harrison, Blaise Dondji, Michael Cappello

Biology Faculty Scholarship

Background

Hundreds of millions of people in poor countries continue to suffer from disease caused by bloodfeeding hookworms. While mice and rats are not reliably permissive hosts for any human hookworm species, adult Golden Syrian hamsters are fully permissive for the human and animal pathogen Ancylostoma ceylanicum. Similar to humans, hamsters may be infected with A. ceylanicum third-stage larvae orally or percutaneously. Oral infection typically leads to consistent worm yields in hamsters but may not accurately reflect the clinical and immunological manifestations of human infection resulting from skin penetration.

Methodology/Principal findings

In this study we compared host responses …

Illinois State University Salaries Fy2022, Illinois State University

Illinois State University Salaries

Includes two sections: non-tenure track, tenure track, and faculty associate salaries, and administrative professional and civil service salaries.

I Just Work Here, Carrie Rudd

Theses and Dissertations

Via an in-depth analysis of the artist’s works and the material, social, and historical possibilities of Painting, the following paper investigates the myriad approaches for Painting to communicate complex ideas both physically and psychologically.

A Liquid Line, Sofía Del Mar Collins

Theses and Dissertations

My practice searches for fertility amidst cultural and material detritus. This paper outlines flows embedded in becomingness. My thesis exhibition included Liquidscapes, a series of suspended and wall hung paintings on plastic, Nursery of the Brave, a group of hanging vessels shaped from waste textiles, and Glass City, a video.

Christ And American Culture- Some Biblical Reflections, Paul Raabe

Concordia Pages

Going beyond H. Richard Neibuhr’s famous paradox model on “Christ and Culture,” Dr. Paul Raabe contends for a biblical, dynamic tension that exists between the culture and the faith. Using the image of a house, Raabe explores four “rooms” : (1) Christianity affirms culture in some respects; (2) Christianity opposes culture in some respects; (3) Christianity leavens culture in some ways; and (4) Christianity uses cultural forms to communicate its message. Each of the four rooms needs to be lived in for maintaining the tension presented in the Scriptures as a faithful witness to the lordship of Christ.

Ph-Responsive Liposomes Of Dioleoyl Phosphatidylethanolamine And Cholesteryl Hemisuccinate For The Enhanced Anticancer Efficacy Of Cisplatin, Hassan Shah, Asadullah Madni, Muhammad Muzamil Khan, Fiaz-Ud-Din Ahmad, Nasrullah Jan, Safiullah Khan, Muhammad Abdur Rahim, Shahzeb Khan, Meser M. Ali, Mohsin Kazi

Neurosurgery Articles

The current study aimed to develop pH-responsive cisplatin-loaded liposomes (CDDP@PLs) via the thin film hydration method. Formulations with varied ratios of dioleoyl phosphatidylethanolamine (DOPE) to cholesteryl hemisuccinate (CHEMS) were investigated to obtain the optimal particle size, zeta potential, entrapment efficiency, in vitro release profile, and stability. The particle size of the CDDP@PLs was in the range of 153.2 ± 3.08-206.4 ± 2.26 nm, zeta potential was -17.8 ± 1.26 to -24.6 ± 1.72, and PDI displayed an acceptable size distribution. Transmission electron microscopy revealed a spherical shape with ~200 nm size. Fourier transform infrared spectroscopic analysis showed the physicochemical stability …

Chronic Background Radiation Correlates With Sperm Swimming Endurance In Bank Voles From Chernobyl, Kati Kivisaari, Sara Calhim, Zbyszek Boratyríski, Timothy Mousseau, Anders P. Møller, Tapio Mappes

Faculty Publications

Sperm quantity and quality are key features explaining intra- and interspecific variation in male reproductive success. Spermatogenesis is sensitive to ionizing radiation and laboratory studies investigating acute effects of ionizing radiation have indeed found negative effects of radiation on sperm quantity and quality. In nature, levels of natural background radiation vary dramatically, and chronic effects of low-level background radiation exposure on spermatogenesis are poorly understood. The Chernobyl region offers a unique research opportunity for investigating effects of chronic low-level ionizing radiation on reproductive properties of wild organisms. We captured male bank voles (Myodes glareolus) from 24 locations in the Chernobyl …

Plasma Induced Reactive Oxygen Species-Dependent Cytotoxicity In Glioblastoma 3d Tumourspheres, Janith Wanigasekara, Carlos Barcia, Patrick J. Cullen, Brijesh Tiwari, James F. Curtin

Articles

The aim of this study was to determine the effects of a pin‐to‐plate cold atmospheric plasma (CAP) on U‐251 MG three‐dimensional (3D) glioblastoma spheroids under different conditions. 3D tumorspheres showed higher resistance to the CAP treatment compared to 2D monolayer cells. A single CAP treatment was able to induce cytotoxicity, while multiple CAP treatments augmented this effect. CAP was also able to induce cytotoxicity throughout the tumoursphere, and we identified that reactive oxygen species(ROS) plays a major role, while H2O2plays a partial role in CAP‐induced cytotoxicity in tumour-spheres. We conclude that ROS‐dependent cytotoxicity is induced uniformly throughout glioblastoma and epidermoid …

Upregulation Of Cd36, A Fatty Acid Translocase, Promotes Colorectal Cancer Metastasis By Increasing Mmp28 And Decreasing E-Cadherin Expression, James Drury, Piotr G. Rychahou, Courtney O. Kelson, Mariah E. Geisen, Yuanyuan Wu, Daheng He, Chi Wang, Eun Y. Lee, B. Mark Evers, Yekaterina Y. Zaytseva

Surgery Faculty Publications

Altered fatty acid metabolism continues to be an attractive target for therapeutic intervention in cancer. We previously found that colorectal cancer (CRC) cells with a higher metastatic potential express a higher level of fatty acid translocase (CD36). However, the role of CD36 in CRC metastasis has not been studied. Here, we demonstrate that high expression of CD36 promotes invasion of CRC cells. Consistently, CD36 promoted lung metastasis in the tail vein model and GI metastasis in the cecum injection model. RNA-Seq analysis of CRC cells with altered expression of CD36 revealed an association between high expression of CD36 and upregulation …

Thrombospondin-1 Expression And Modulation Of Wnt And Hippo Signaling Pathways During The Early Phase Of Trypanosoma Cruzi Infection Of Heart Endothelial Cells, Ashutosh Arun, Kayla J. Rayford, Ayorinde Cooley, Tanu Rana, Girish Rachakonda, Fernando Villalta, Siddharth Pratap, Maria F. Lima, Nader Sheibani, Pius N. Nde

Publications and Research

The protozoan parasite, Trypanosoma cruzi, causes severe morbidity and mortality in afflicted individuals. Approximately 30% of T. cruzi infected individuals present with cardiac pathology. The invasive forms of the parasite are carried in the vascular system to infect other cells of the body. During transportation, the molecular mechanisms by which the parasite signals and interact with host endothelial cells (EC) especially heart endothelium is currently unknown. The parasite increases host thrombospondin-1 (TSP1) expression and activates the Wnt/β-catenin and hippo signaling pathways during the early phase of infection. The links between TSP1 and activation of the signaling pathways and …

Evaluating A Targeted Cancer Therapy Approach Mediated By Rna, Katharina Woess, Yuchen Sun, Hanae Morio, Anna Stierschneider, Anna Kaufmann, Stefan Hainzl, Lisa Trattner, Thomas Kocher, Birgit Tockner, Victoria Leb-Reichl, Markus Steiner, Gabriele Brachtl, Andrew P. South, Johann W Bauer, Julia Reichelt, Tomomi Furihata, Verena Wally, Ulrich Koller, Josefina Piñón Hofbauer, Christina Guttmann-Gruber

Department of Dermatology and Cutaneous Biology Faculty Papers

Conventional anti-cancer therapies based on chemo- and/or radiotherapy represent highly effective means to kill cancer cells but lack tumor specificity and, therefore, result in a wide range of iatrogenic effects. A promising approach to overcome this obstacle is spliceosome-mediated RNA trans-splicing (SMaRT), which can be leveraged to target tumor cells while leaving normal cells unharmed. Notably, a previously established RNA trans-splicing molecule (RTM44) showed efficacy and specificity in exchanging the coding sequence of a cancer target gene (Ct-SLCO1B3) with the suicide gene HSV1-thymidine kinase in a colorectal cancer model, thereby rendering tumor cells sensitive to the prodrug ganciclovir (GCV). In …

Industry 4.0 Technologies For The Manufacturing And Distribution Of Covid-19 Vaccines, Azza Sarfraz, Zouina Sarfraz, Muzna Sarfraz, Aminah Abdul Razzack, Shehar Bano, Sarabjot Singh Makkar, Sindhu Thevuthasan, Trissa Paul, Muhammad Khawar Sana, Nishwa Azeem

Department of Paediatrics and Child Health

Background: The evolutionary stages of manufacturing have led us to conceptualize the use of Industry 4.0 for COVID-19 (coronavirus disease 2019), powered by Industry 4.0 technologies. Using applications of integrated process optimizations reliant on digitized data, we propose novel intelligent networks along the vaccine value chain. Vaccine 4.0 may enable maintenance processes, streamline logistics, and enable optimal production of COVID-19 vaccines.
Vaccine 4.0 framework: The challenge in applying Vaccine 4.0 includes the requirement of large-scale technologies for digitally transforming manufacturing, producing, rolling-out, and distributing vaccines. With our framework, Vaccine 4.0 analytics will target process performance, process development, process stability, compliance, …

Aav5 Delivery Of Crispr-Cas9 Supports Effective Genome Editing In Mouse Lung Airway, Shun-Qing Liang, Christopher J Walkey, Alexa E Martinez, Qin Su, Mary E Dickinson, Dan Wang, William R Lagor, Jason D Heaney, Guangping Gao, Wen Xue

Faculty, Staff and Students Publications

Genome editing in the lung has the potential to provide long-term expression of therapeutic protein to treat lung genetic diseases. Yet efficient delivery of CRISPR to the lung remains a challenge. The NIH Somatic Cell Genome Editing (SCGE) Consortium is developing safe and effective methods for genome editing in disease tissues. Methods developed by consortium members are independently validated by the SCGE small animal testing center to establish rigor and reproducibility. We have developed and validated a dual adeno-associated virus (AAV) CRISPR platform that supports effective editing of a lox-stop-lox-Tomato reporter in mouse lung airway. After intratracheal injection of the …

Estuarine Infauna Within Incidentally Retained Sediment In Artificial Rockpools, Jessica R. Bone, Rick Stafford, Alice E. Hall, Ian Boyd, Nigel George, Roger J.H. Herbert

School of Biological and Marine Sciences

Artificial coastal structures (ACSs) are primarily designed to provide services for human use, such as flood defence or shipping, and are generally poor for marine biodiversity. Consequently, there has been significant research effort to enhance these hard structures to increase biodiversity and habitat availability via eco-engineering. On seawalls and breakwaters, this has included the creation of habitats for benthic species found on natural rocky shores, including the provision of cracks, crevices and water retaining features, such as artificial rockpools. When sediment retention in these features has occurred, it has often been deemed detrimental to the overarching aim of the intervention. …