Digital Commons Network^™

Indole-3-Glycerol Phosphate Synthase From Mycobacterium Tuberculosis: A Potential New Drug Target, Nikolas Esposito, David W. Konas, Nina M. Goodey

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

Tuberculosis (TB), caused by the pathogen Mycobacterium tuberculosis, affects millions of people worldwide. Several TB drugs have lost efficacy due to emerging drug resistance and new anti-TB targets are needed. Recent research suggests that indole-3-glycerol phosphate synthase (IGPS) in M. tuberculosis (MtIGPS) could be such a target. IGPS is a (β/α)8-barrel enzyme that catalyzes the conversion of 1-(o-carboxyphenylamino)-1-deoxyribulose 5’-phosphate (CdRP) into indole-glycerol-phosphate (IGP) in the bacterial tryptophan biosynthetic pathway. M. tuberculosis over expresses the tryptophan pathway genes during an immune response and inhibition of MtIGPS allows CD4 T-cells to more effectively fight against M. tuberculosis. Here we review the published …

Genetic Parameters, Heterosis, And Breed Effects For Body Condition Score And Mature Cow Weight In Beef Cattle, André Mauric F. Ribeiro, Leticia P. Sanglard, Warren M. Snelling, R. Mark Thallman, Larry A. Kuehn, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Understanding the genetic relationship between mature cow weight (MWT) and body condition score (BCS) is useful to implement selection programs focused on cow efficiency. The objectives of this study were to estimate genetic parameters, heterosis, and breed effects for MWT and BCS. In total, 25,035 and 24,522 overlapping records were available for MWT and BCS on 6,138 and 6,131 cows, respectively, from the Germplasm Evaluation program, a crossbred beef population at the U.S. Meat Animal Research Center. Pedigree was available for 48,013 individuals. Univariate animal models were used to estimate heritabilities for each trait by parity. …

A Muscle Cell-Macrophage Axis Involving Matrix Metalloproteinase 14 Facilitates Extracellular Matrix Remodeling With Mechanical Loading, Bailey D. Peck, Kevin A. Murach, R. Grace Walton, Alexander J. Simmons, Douglas E. Long, Kate Kosmac, Cory M. Dungan, Philip A. Kern, Marcas M. Bamman, Charlotte A. Peterson

Center for Muscle Biology Faculty Publications

The extracellular matrix (ECM) in skeletal muscle plays an integral role in tissue development, structural support, and force transmission. For successful adaptation to mechanical loading, remodeling processes must occur. In a large cohort of older adults, transcriptomics revealed that genes involved in ECM remodeling, including matrix metalloproteinase 14 (MMP14), were the most upregulated following 14 weeks of progressive resistance exercise training (PRT). Using single-cell RNA-seq, we identified macrophages as a source of Mmp14 in muscle following a hypertrophic exercise stimulus in mice. In vitro contractile activity in myotubes revealed that the gene encoding cytokine leukemia inhibitory factor ( …

The Directed Steps Of Hans Christian Gram: How Providence Played A Role In The Gram Stain, Which Illumines The Ordered Pattern Of Bacterial Cell Walls And Helps Identify Pathogens Of Infectious Diseases, Alan L. Gillen

Faculty Publications and Presentations

This paper honors Hans Christian Joachim Gram. In published papers, he simply identified as Christian Gram. Gram devised a staining technique that is still used to identify and classify different types of bacteria. He was a Danish microbiologist, born in Copenhagen on September 13, 1853. In German microbiologist Karl Friedländer’s lab, Gram noticed that staining a smear of pneumonia bacteria with a crystal violet followed by iodine and organic solvent showed differences in various samples. “Gram-negative” bacteria have thin cell walls that allow the solvent to wash away part of the stain. “Gram-positive” bacteria appear purple in microscopy because their …

Materials And Methodologies For Spectroscopic And Optical Analytical Applications In Cultural Heritage Conservation Science, Lyndsay Nichole Kissell

Dissertations and Theses

The field of conservation science falls in the intersection of science and art. The work of conservation scientists may include any single subdiscipline of chemistry, though it is most commonly a highly interdisciplinary field taking skills from analytical, organic, and inorganic chemistry, as well as surface and materials science. The aims of conservation scientists are to answer questions about the production and aging of material cultural heritage. Knowing the materials used by an artist can lead to insight about the intentions of the object and knowing how those materials degrade will enable the use of preventative measures to ensure the …

The Parthenon, January 19, 2022, Tyler Spence, Conner Woodruff, Tyler Kennett, Evan Green, Shauntelle Thompson, Rafael Alfonso, Abby Hanlon, Abby Ayes, Alaiana Laster, Isabella Robinson

The Parthenon

No abstract provided.

Short Chain Fatty Acids Taken At Time Of Thrombectomy In Acute Ischemic Stroke Patients Are Independent Of Stroke Severity But Associated With Inflammatory Markers And Worse Symptoms At Discharge, Nicholas Henry, Jacqueline A. Frank, Christopher J. Mclouth, Amanda L. Trout, Andrew J. Morris, Jianzhong Chen, Ann M. Stowe, Justin F. Fraser, Keith R. Pennypacker

Behavioral Science Faculty Publications

Introduction: Short chain fatty acids (SCFA) are gut microbiota-derived metabolites that contribute to the gut-brain axis and may impact stroke outcomes following gut dysbiosis. We evaluated plasma SCFA concentrations against stroke severity parameters and identified SCFA-associated protein networks.

Methods: The Blood and Clot Thrombectomy Registry and Collaboration (BACTRAC), a continuously enrolling tissue bank, was used to obtain stroke samples. Arterial blood distal and proximal to the thrombus was obtained from Acute Ischemic Stroke (AIS) Patients (n=53) during thrombectomy. Patient demographics, stroke presentation and outcome parameters were reported. The SCFAs were isolated from proximal plasma via chemical derivatization UHPLC coupled tandem …

Crop Rotational Complexity Affects Plant-Soil Nitrogen Cycling During Water Deficit, Timothy M. Bowles, Andrea Jilling, Karen Moran-Rivera, Jorg Schnecker, A. Stuart Grandy

Faculty Publications

One of the biggest environmental challenges facing agriculture is how to both supply and retain nitrogen (N), especially as precipitation becomes more variable with climate change. We used a greenhouse experiment to assess how contrasting histories of crop rotational complexity affect plant-soil-microbe interactions that govern N processes, including during water stress. With higher levels of carbon and N cycling hydrolytic enzymes, higher mineral-associated organic matter N concentrations, and an altered microbial community, soils from the most complex rotation enabled 80% more corn N uptake under two moisture regimes, compared to soil from monoculture corn. Higher levels of plant N likely …

Microplastics Through The Lens Of Colloid Science, Ahmed Al Harraq, Bhuvnesh Bharti

Faculty Publications

Microplastics are sub-millimeter-sized fragments of plastics and a relatively new class of pollutant increasingly found in the environment. Due to their size and surface area to volume ratio, the physicochemical characteristics of microplastics can diverge from those of their macroscopic counterparts. This is partly why it is challenging to understand their origin, analyze their behavior, and predict their fates in the environment compared to large pollutants. We believe that adopting a view of microplastics as a colloid provides a holistic framework that connects their physical properties and surface chemistries with observations of their dynamics in the environment. In particular, we …

Ecology Of Periphyton In A Subtropical River Floodplain, Kamela De Gallardo

LSU Doctoral Dissertations

The Atchafalaya River Basin (ARB) in southcentral Louisiana, USA, is a large and biologically diverse floodplain surrounding the Atchafalaya River (AR), which is the largest distributary of the Mississippi River, receiving 30% of the combined daily discharge of the Mississippi and Red Rivers. Annual flooding facilitates exchange between the AR and its floodplain and is thought to give rise to the high productivity of the river-floodplain system. Primary production within the aquatic ARB is driven by periphytic algae, phytoplankton, and aquatic macrophytes, however, very little is known about periphytic algal assemblages in floodplain systems. In this study, artificial substrates were …

Comparative Study On The Chronic Vascular Responses Induced By Regular Versus Occasional Waterpipe Smoke Inhalation In Mice, Naserddine Hamadi, Sumaya Beegam, Nur Elena Zaaba, Ozaz Elzaki, Badreldin H Ali, Abderrahim Nemmar

All Works

BACKGROUND/AIMS: Waterpipe smoke (WPS) is the second most prevalent form of smoking in the world. There are ample evidences about the vascular alterations caused by regular WPS (Reg-WPS). Nonetheless, comparison of the chronic vascular response induced by regular versus occasional WPS (Occ-WPS) exposure is very scarce. METHODS: We investigated, in BALB/c mice, the effects of Occ-WPS (30 minutes/day, 1 day/week) versus Reg-WPS (30 minutes/day, 5 days/week) for 6 months on thrombogenicity and platelet aggregation in vivo and in vitro. Moreover, various markers of endothelial integrity, inflammation and oxidative stress were assessed by enzyme-linked immunosorbent assay and colorimetric assay. Control mice …

Spectroscopic Properties Relevant To Astronomical And Laboratory Detection Of Mch And Mch+ (M = Al, Mg), Vincent J. Esposito, Tarek Trabelsi, Joseph S. Francisco

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

New spectroscopic parameters have been calculated to aid in the laboratory and observational detection of AlCH, AlCH⁺, MgCH, MgCH⁺, and their deuterated analogs. All species exist in linear geometry except for AlCH+, which exhibits a bent structure. Rotational frequencies have been calculated and the transition with maximum intensity is discussed for three temperature regions present in space: 100 K for a central circumstellar envelope (CSE), 30 K for an outer CSE, and 3 K indicative of the interstellar medium. Pertinent frequencies range from 25 to 250 GHz, depending on the species. At 30 K, the most …

Prenatal Exposure To Maternal Psychological Distress And Telomere Length In Childhood, Stephanie A. Stout-Oswald, Laura M. Glynn, Marco Bisoffi, Catherine H. Demers, Elysia Poggi Davis

Psychology Faculty Articles and Research

Telomere length (TL) is a biological marker of cellular aging, and shorter TL in adulthood is associated with increased morbidity and mortality risk. It is likely that these differences in TL are established long before adulthood, and there is growing evidence that TL can reflect prenatal experiences. Although maternal prenatal distress predicts newborn TL, it is unknown whether the relation between prenatal exposure to maternal distress and child TL persists through childhood. The purpose of the current longitudinal, prospective study is to examine the relation between prenatal exposure to maternal distress (perceived stress, depressive symptoms, pregnancy-related anxiety) and TL in …

Chemical Weapons And Their Unforeseen Impact On Health And The Environment, Alexandra Chen

Seattle Journal of Technology, Environmental, & Innovation Law

The May 2020 police murder of George Floyd catalyzed a racial reckoning in the United States that saw millions of people take to the streets to protest police brutality against people of color. In following months, law enforcement used massive amounts of "less-lethal" chemical weapons against protesters in cities across the country. Despite widespread use of chemical weapons by police agencies and mounting evidence of related environmental and health harms, the federal government does not regulate the use nor the manufacture of chemical weapons. Chemical weapons contain toxic ingredients such as hexavalent chromium, lead salts, and methylene chloride, which are …

Uranium 233: The Nuclear Superfuel No One Is Using, Maris Hanson

Seattle Journal of Technology, Environmental, & Innovation Law

Nuclear power offers more energy in less physical space than solar and wind and yields more energy per pound than fossil fuels. However different nuclear fuels yield different waste profiles and create different beneficial products. Uranium 233 (U233) resists use in nuclear weapons, yields beneficial daughter products, and produces dramatically less of the most problematic waste products than Uranium 235 (U235). U233 results from reactions with Thorium, a plentiful, ubiquitous element currently considered waste from rare earth mines. Additionally, U233 functions well in a liquid fuel reactor resulting in safer, more efficient reactors than current solid fuel U235 or Plutonium …

The Gut Microbiota Of Chickens In A Commercial Farm Treated With A Salmonella Phage Cocktail, Viviana Clavijo, Tatiana Morales, Martha Josefina Vives-Flores, Alejandro Reyes Muñoz

Open Access Publications

The microbiota in broiler chicken intestines affects the animals' health, metabolism, and immunity both positively and negatively. Accordingly, it has a significant impact on animal productivity. Phages, host-specific parasites of bacterial cells, are a promising antimicrobial alternative that selectively target pathogens without disturbing the microbiota. The purpose of this study is to further characterize the commensal microbial community at production scale in broiler chickens treated with a Salmonella phage treatment. We evaluated the cecal microbiota of broilers reared in a commercial farming system where a phage cocktail against Salmonella, SalmoFree was supplied to animals. To do so, two field trials …

Niche Diversity Predicts Personality Structure Across 115 Nations, Patrick K. Durkee, Aaron W. Lukaszewski, Christopher Von Rueden, Michael D. Gurven, David M. Buss, Elliot M. Tucker-Drob

Jepson School of Leadership Studies articles, book chapters and other publications

The niche-diversity hypothesis proposes that personality structure arises from the affordances of unique trait combinations within a society. It predicts that personality traits will be both more variable and differentiated in populations with more distinct social and ecological niches. Prior tests of this hypothesis in 55 nations suffered from potential confounds associated with differences in the measurement properties of personality scales across groups. Using psychometric methods for the approximation of cross-national measurement invariance, we tested the niche-diversity hypothesis in a sample of 115 nations (N = 685,089). We found that an index of niche diversity was robustly associated with …

The Lived Experience In The Clinical Setting Of Nursing Students With Disabilities, Amy Luckowski

Dissertations

With the implementation of the 504 Rehabilitation Act, the American with Disabilities Act of 1990 (ADA), and the ADA Amendments Act of 2008 (ADAAA), more students with disabilities are being admitted into universities and into nursing programs. The purpose of this qualitative study was to explore the experiences in the clinical setting of nursing students with disabilities. Moustakas's psychological phenomenology was utilized to describe the meaning of the student's experiences. A purposeful sample of 13 junior and senior baccalaureate nursing students with disabilities from two northeastern baccalaureate nursing programs who had at least one semester of clinical experience were interviewed …

Sustainability Of Mahogany Production In Plantations: Does Resource Availability Influence Susceptibility Of Young Mahogany Plantation Stands To Hypsipyla Robusta Infestation?, C. B. Darko, E. Opuni-Frimpong, S. A. Owusu, B. Kyere, A. J. Storer

Michigan Tech Publications, Part 1

Hypsipyla robusta Moore (Lepidoptera: Pyralidae), like many other moth species, shows selectivity when choosing host plants for its eggs. Four Meliaceae species (Khaya grandifoliola, K. ivorensis, Swietenia macrophyla, and Entandrophragma cylindricum) were established in a moist semideciduous forest in Ghana to study this selectivity at 12 and 21 months after planting. The analysis of variance (ANOVA) at a P-value of 0.05 was used to test the significance of differences in infestation by H. robusta between the species. H. robusta attacks were recorded by month 12 after planting in the field, and only Khaya spp. was attacked, with attacks evident on …

The Impact Of Aging On The Lung Alveolar Environment, Predetermining Susceptibility To Respiratory Infections, Jordi B. Torrelles, Blanca I. Restrepo, Yidong Bai, Corinna Ross, Larry S. Schlesinger, Joanne Turner

School of Medicine Publications

Respiratory infections are one of the top causes of death in the elderly population, displaying susceptibility factors with increasing age that are potentially amenable to interventions. We posit that with increasing age there are predictable tissue-specific changes that prevent the immune system from working effectively in the lung. This mini-review highlights recent evidence for altered local tissue environment factors as we age focusing on increased tissue oxidative stress with associated immune cell changes, likely driven by the byproducts of age-associated inflammatory disease. Potential intervention points are presented.

Seasonality And Grazing Management Effect On Growth And Nutritional Composition Of Herbage On Semi-Natural Grasslands Grazed By Dairy Cows In Southwest Germany, E. Velasco, S. Perdana-Decker, J. Werner, U. Dickhoefer

IGC Proceedings (1977-2023)

Natural and semi-natural grasslands represent an attractive forage source for cows in organic dairy farming. Throughout the grazing season, however, diverse factors such as climatic conditions, botanical composition, as well as grazing and herd management, can influence growth and nutritive value of forage on grasslands. We aimed at investigating the influence of seasonality and grazing management in forage growth and nutritional quality in seven commercial organic dairy cattle farms during the grazing season 2019 in South Germany. Therefore, forty exclusion cages (1 m²) were installed on pastures, which were under three different grazing systems: rotational, short-grass, and continuous …

Effect Of Cutting Stage And Turning Frequency On Nutritive Value And Digestibility Of Two Tropical Forages, M. M. Chiphwanya, F. C. Chigwa, H. D. C. Msiska

IGC Proceedings (1977-2023)

In Malawi, feed shortage during the dry season (June-October) is a critical factor affecting smallholder dairying. Though suitable improved forages were long introduced, important parameters that indicate forage and hay quality for dry season feeding have been less well studied. This study evaluated the effect of cutting stage and turning frequency at drying of two tropical forages on nutrient composition and digestibility. A 2³ factorial experiment laid out in Randomized Complete Block Design was conducted in Mzuzu milk shed area. Centrosema pubescens and Chloris gayana were established on four plots (5m x 3m) each by 24 dairy farmers; two …

Public Health Equity Law: Considering Law As A Tool In Achieving Health Equity, Dawn Pepin

DePaul Law Review

No abstract provided.

Pbis At Upson-Lee: The 'Knight' Way, Matthew C. Bentley, Amanda Miller, Alan Hendrick, Carolyn Patterson

Georgia Association for Positive Behavior Support Conference

As we lead Upson-Lee High School through these difficult times, we know that the culture of our school plays a huge role in the success of our students. We use our PBIS program to help develop student leaders that will model dispositions of initiative, enthusiasm, scholarship, and self-regulation. These students create the roots for student success in school, casting seeds for their peers to think about school in a new light. Our goal is for all students to know that school is a place where they belong and a place where the adults with whom they spend their day, truly …

Effect Of Cutting Height On Productivity And Quality Of Alfalfa In Northern Area Of Korea, Jong Geun Kim, C. Liu, S. N. Wei, Y. F. Li, E. C. Jeong, H. J. Kim

IGC Proceedings (1977-2023)

Alfalfa is a very important forage for livestock feeding. In particular, due to the high crude protein content, it is widely used as a dairy cattle feeds. However, the cultivation of alfalfa is quite limited and main source is imported hay in Korea. There are many reasons, but most importantly, low productivity due to lack of boron in the soil. This experiment was conducted to expand domestic production and use of alfalfa. Alfalfa was sown in the fall of 2018 at 30 kg/ha seeding rate. Alfalfa was harvested four times (3 May, 2 July, 11 September and 13 October) in …

Economic Injury Level For Bermudagrass Stem Maggot (Diptera: Muscidae) In Bermudagrass Forage Production In Texas, V. Corriher-Olson, A. Knutson, F. Mitchell

IGC Proceedings (1977-2023)

The bermudagrass stem maggot, Atherigona reversura Villeneuve, was first reported damaging bermudagrass Cynodon dactylon (L.) Pers grown for forage in 2010 in the southeastern US. Damage results from individual larvae feeding internally on the vascular tissue just above the terminal node of the grass stem. Damage slows plant growth and reduces forage accumulation. To address the need for economic guidelines to manage this new pest, the relationship between the percent of stems damaged by bermudagrass stem maggot and forage yield was measured in commercial bermudagrass hay fields in northcentral Texas during 2016 and 2017. The slope of the linear regression, …

Dynamic Correlation Estimators For Bivariate Brownian And Geometric Brownian Motions, Majnu John, Yihren Wu

Journal of Stochastic Analysis

No abstract provided.

Encounters Between The Elderly And Law Enforcement

Contemporary Southern Psychology

The elderly population is growing dramatically throughout the world. Out of this growth comes an increase in the number of encounters between the aged and law enforcement. These encounters occur because of a variety of factors including mental illnesses and addictions. Furthermore, older adults may be victims as evidenced in different forms of abuse such as physical, emotional, or financial abuse. Sadly, some documentation exists that older adults are committing more crimes. Multiple reasons have been postulated for these crimes including poverty, jealousy, and boredom. All of these different situations with the aged have created an increase in the number …

Diversity And Taxonomic Identity Of Rumen Bacterial Community In Cattle Fed Different Diets, B. K. Korir, J. K. N. Kuria, D. M. Mwangi, M. M. M. Wanyoike

IGC Proceedings (1977-2023)

In this study, the identity of rumen bacterial community and their taxonomic classification in Zebu heifers (n=24) fed 4 different diets (range grasses, Bracharia MulatoII, Azolla and Cassava leaf meal) was surveyed using metagenomics sequencing of the 16SrRNA gene. Rumen liquor samples were collected from the heifers from which a total of 192 DNA samples were amplified and the resulting 16S rRNA sequences compared to the existing sequences at the National Centre for Biotechnology Information (NCBI) BLAST database through the MetagenAssist . Bioinformatics analyses indicated that 17 operational taxonomic units (OTUs) were present at the phylum level. Of these, 43.3% …

Current Status And Future Strategy In Megathyrsus Maximus Breeding Program At The Alliance Bioversity - Ciat, L. M. Hernandez, V. Castiblanco

IGC Proceedings (1977-2023)

Megathyrsus maximus (Jacq.) B.K. Simon & S.W.L. Jacobs is one of the most important grass species cultivated for cattle production in cut and carry systems. There is a need to search for superior hybrids with high potential for mitigation and adaptation to climate change. A big proportion of the M. maximus germplasm, preserved in the CIAT GeneBank has been agronomically characterized and genotypes with desirable traits such as high drought tolerance, high BNI potential (Biological nitrification inhibition), high nutritional quality (biomass production, dry matter, crude protein, acid detergent fiber, neutral detergent fiber and dry matter digestibility) and high seed production …