Digital Commons Network^™

Blood-Brain Barrier Disruption Mediated By Ffa1 Receptor-Evidence Using Miniscope, Kristen L Lindenau, Jeffrey L Barr, Christopher R Higgins, Kevin T Sporici, Eugen Brailoiu, G Cristina Brailoiu

College of Pharmacy Faculty Papers

Omega-3 polyunsaturated fatty acids (n-3 PUFAs), obtained from diet and dietary supplements, have been tested in clinical trials for the prevention or treatment of several diseases. n-3 PUFAs exert their effects by activation of free fatty acid (FFA) receptors. FFA1 receptor, expressed in the pancreas and brain, is activated by medium- to long-chain fatty acids. Despite some beneficial effects on cognition, the effects of n-3 PUFAs on the blood-brain barrier (BBB) are not clearly understood. We examined the effects of FFA1 activation on BBB permeability in vitro, using rat brain microvascular endothelial cells (RBMVEC), and in vivo, by assessing Evans …

Factors Affecting Student Engagement In Online Teaching And Learning: A Qualitative Case Study, Basilius R. Werang Brw, Seli Marlina Radja Leba

The Qualitative Report

Students’ engagement is a decisive prerequisite for effective teaching and learning. Yet, the discussion on students’ engagement in online teaching and learning is still limited. In this study, we explore online lecturers’ perceptions of factors affecting student engagement in online teaching and learning offered at Musamus University, Indonesia. To reach this objective, we employed a qualitative case study using in-depth interviews with 10 online lecturers. Obtained data were thematically analyzed. Results of data analysis revealed that students’ lack of access to a personal computer or smartphone, as well as the internet, were found to be the most significant factors affecting …

Release Of Chk-2 From Ppm-1.D Anchorage Schedules Meiotic Entry, Antoine Baudrimont, Dimitra Paouneskou, Ariz Mohammad, Raffael Lichtenberger, Joshua Blundon, Yumi Kim, Markus Hartl, Sebastian Falk, Tim Schedl, Verena Jantsch

Open Access Publications

Transition from the stem/progenitor cell fate to meiosis is mediated by several redundant posttranscriptional regulatory pathways in

Human Milk Oligosaccharide Compositions Illustrate Global Variations In Early Nutrition, Anita Vinjamuri, Jasmine C. C. Davis, Sarah M. Totten, Lauren D. Wu, Laura D. Klein, Melanie Martin, Ea Quinn, Brooke Scelza, Alicia Breakey, Michael Gurven, Grazyna Jasienska, Hillard Kaplan, Claudia Valeggia, Katie Hinde, Jennifer T. Smilowitz, Robin M. Bernstein, Angela M. Zivkovic, Michael J. Barratt, Jeffrey I. Gordon, Mark A. Underwood, David A. Mills, J. Bruce German, Carlito B. Lebrilla

ESI Publications

Background

Human milk oligosaccharides (HMOs) are an abundant class of compounds found in human milk and have been linked to the development of the infant, and specifically the brain, immune system, and gut microbiome.

Objectives

Advanced analytical methods were used to obtain relative quantitation of many structures in approximately 2000 samples from over 1000 mothers in urban, semirural, and rural sites across geographically diverse countries.

Methods

LC-MS−based analytical methods were used to profile the compounds with broad structural coverage and quantitative information. The profiles revealed their structural heterogeneity and their potential biological roles. Comparisons of HMO compositions were made between …

Blood–Brain Barrier Disruption Mediated By Ffa1 Receptor—Evidence Using Miniscope, Kristen L Lindenau, Jeffrey L Barr, Christopher R Higgins, Kevin T Sporici, Eugen Brailoiu, Gabriela C Brailoiu

Faculty, Staff and Student Publications

Omega-3 polyunsaturated fatty acids (n-3 PUFAs), obtained from diet and dietary supplements, have been tested in clinical trials for the prevention or treatment of several diseases. n-3 PUFAs exert their effects by activation of free fatty acid (FFA) receptors. FFA1 receptor, expressed in the pancreas and brain, is activated by medium- to long-chain fatty acids. Despite some beneficial effects on cognition, the effects of n-3 PUFAs on the blood-brain barrier (BBB) are not clearly understood. We examined the effects of FFA1 activation on BBB permeability in vitro, using rat brain microvascular endothelial cells (RBMVEC), and in vivo, by assessing Evans …

Health Promotion In An Australian Aboriginal Community: The Growing Strong Brains ® Toolkit, Wendy Simpson, Darlene Robinson, Elaine Bennett, Cecily Strange, Vicki Banham, Jenny Allen, Rhonda Marriott

Research outputs 2022 to 2026

AIM: The aim of this paper is to describe the implementation and evaluation of the Growing Strong Brains® (GSB) toolkit in a remote Aboriginal community in Western Australia (WA) over a 2-year period, 2018-2019. BACKGROUND: Ngala, a community service organisation in WA, developed the GSB toolkit in 2014, a culturally appropriate and interactive resource to build knowledge of early childhood development within Aboriginal communities. This was in response to evidence that a higher percentage of children in Aboriginal communities were developmentally vulnerable compared to the rest of the population. The GSB toolkit promotes awareness and understanding of early brain development …

Big-Data Approaches Lead To An Increased Understanding Of The Ecology Of Animal Movement, Mark A. Whiteside

School of Biological and Marine Sciences

No abstract provided.

Adrenergic Modulation Of Precursor Cells Of Ovarian Cancer, Sweta Dash

USF Tampa Graduate Theses and Dissertations

Ovarian cancer remains one of the most lethal gynecological cancer and ranks eighth in cancer-related mortality among women. The high mortality rate can be attributed to majority of the cases being diagnosed at advanced stages of the disease when the survival rate and overall prognosis are poor. There is lack of effective screening modalities to detect ovarian cancer at early stages. Hence, a better understanding of the molecular mechanisms leading to ovarian cancer initiation is required.

Epidemiological studies have reported that conditions which cause chronic behavioral stress are associated with a higher risk of developing ovarian cancer. The activation of …

Reducing Adverse Impacts Of Amazon Hydropower Expansion, Alexander S. Flecker, Qinru Shi, Rafael M. Almeida, Héctor Angarita, Jonathan M. Gomes-Selman, Roosevelt García-Villacorta, Suresh A. Sethi, Steven A. Thomas, N Leroy Poff, Bruce R. Forsberg

School of Earth, Environmental, & Marine Sciences Faculty Publications

Proposed hydropower dams at more than 350 sites throughout the Amazon require strategic evaluation of trade-offs between the numerous ecosystem services provided by Earth's largest and most biodiverse river basin. These services are spatially variable, hence collective impacts of newly built dams depend strongly on their configuration. We use multiobjective optimization to identify portfolios of sites that simultaneously minimize impacts on river flow, river connectivity, sediment transport, fish diversity, and greenhouse gas emissions while achieving energy production goals. We find that uncoordinated, dam-by-dam hydropower expansion has resulted in forgone ecosystem service benefits. Minimizing further damage from hydropower development requires considering …

Limited Impact Of The Protein Corona On The Cellular Uptake Of Pegylated Zein Micelles By Melanoma Cancer Cells, Jitkasem Meewan, Sukrut Somani, Partha Laskar, Craig Irving, Margaret Mullin, Stuart Woods, Craig W. Roberts, Abdullah R. Alzahrani, Valerie A. Ferro, Suzanne Mcgill

School of Medicine Publications

The formation of a protein layer "corona" on the nanoparticle surface upon entry into a biological environment was shown to strongly influence the interactions with cells, especially affecting the uptake of nanomedicines. In this work, we present the impact of the protein corona on the uptake of PEGylated zein micelles by cancer cells, macrophages, and dendritic cells. Zein was successfully conjugated with poly(ethylene glycol) (PEG) of varying chain lengths (5K and 10K) and assembled into micelles. Our results demonstrate that PEGylation conferred stealth effects to the zein micelles. The presence of human plasma did not impact the uptake levels of …

A Mixed-Methods Exploration Study On Trauma-Informed Practices Used To Dismantle The School-To-Prison Pipeline In Public And Private Schools In The City Of St. Louis, Jennifer Lauren Gabrian

Dissertations

Since the release of the Missouri Model for Trauma-Informed Schools approved by the Department of Elementary and Secondary Education (DESE) in 2019, school leaders were given the opportunity to start the process of meeting the emotional and fundamental needs of students. With the effects of trauma reaching all diverse groups of race, gender, economic status, and community surroundings, there is an increased need to use the education system to reach students emotionally. Though the model is a blueprint for schools, school districts are not required to adopt this model and implement it within their districts. Due to the recent pandemic, …

Syndecan-2 Regulates Pad2 To Exert Antifibrotic Effects On Ra-Ild Fibroblasts, Konstantin Tsoyi, Anthony J Esposito, Bo Sun, Ryan G Bowen, Kevin Xiong, Fernando Poli, Rafael Cardenas, Sarah G Chu, Xiaoliang Liang, Stefan W Ryter, Christine Beeton, Tracy J Doyle, Matthew J Robertson, Lindsay J Celada, Freddy Romero, Souheil Y El-Chemaly, Mark A Perrella, I-Cheng Ho, Ivan O Rosas

Faculty, Staff and Students Publications

Rheumatoid arthritis (RA)-associated interstitial lung disease (RA-ILD) is the most common pulmonary complication of RA, increasing morbidity and mortality. Anti-citrullinated protein antibodies have been associated with the development and progression of both RA and fibrotic lung disease; however, the role of protein citrullination in RA-ILD remains unclear. Here, we demonstrate that the expression of peptidylarginine deiminase 2 (PAD2), an enzyme that catalyzes protein citrullination, is increased in lung homogenates from subjects with RA-ILD and their lung fibroblasts. Chemical inhibition or genetic knockdown of PAD2 in RA-ILD fibroblasts attenuated their activation, marked by decreased myofibroblast differentiation, gel contraction, and extracellular matrix …

Exploring Data And Literature Currently Available On The Covid-19 Vaccines, Pooja Gogia, Hamza Ashraf, Sidharth Bhasin, Douglas Frenia

Journal of Community Hospital Internal Medicine Perspectives

The COVID-19 pandemic has been labeled one of the most lethal pandemics in human history. As a result, there has been a high level of urgency throughout the world to establish successful vaccinations to subdue the effects of the virus and return to a level of normalcy. This study aims to investigate the different COVID-19 vaccines available both in the United States and across the globe. Through exploration of how the vaccines were developed, how they elicit immunity, their efficacy, and their safety profiles, this study has the goal of increasing the amount of knowledge regarding the vaccines available to …

Seizure Prediction In Epilepsy Patients, Gary Dean Cravens

NSU REACH and IPE Day

Purpose/Objective: Characterize rigorously the preictal period in epilepsy patients to improve the development of seizure prediction techniques. Background/Rationale: 30% of epilepsy patients are not well-controlled on medications and would benefit immensely from reliable seizure prediction. Methods/Methodology: Computational model consisting of in-silico Hodgkin-Huxley neurons arranged in a small-world topology using the Watts-Strogatz algorithm is used to generate synthetic electrocorticographic (ECoG) signals. ECoG data from 18 epilepsy patients is used to validate the model. Unsupervised machine learning is used with both patient and synthetic data to identify potential electrophysiologic biomarkers of the preictal period. Results/Findings: The model has shown states corresponding to …

Contributor Notes

Exile

No abstract provided.

A Systematic Review To Propose An Evidence-Based Nutrition Protocol In Depression, Camilla A. Mirkin, Nicole Koci

NSU REACH and IPE Day

Background: Rates of depression have been rising steadily over the last several decades with more than 264 million people affected, making it one of the top three burdens globally. The pandemic has brought on a greater risk of depression. Forty-two percent of individuals surveyed in December 2020 by the US Census Bureau reported depressive symptoms. Many factors, including poor diet quality, play a role in the development of depression during traumatic events like the pandemic. A low-quality dietary pattern has been associated with obesity, low-grade chronic inflammation, dysbiosis, increased cortisol levels and monoamine neurotransmitter deficiency. Among others, these factors have …

Toward A Crip Provenance: Centering Disability In Archives Through Its Absence, Gracen M. Brilmyer

Journal of Contemporary Archival Studies

Using the records that document the 1893 World’s Columbian Exposition as a case study, this article discusses the messiness and unknowability of provenance. Drawing attention to how the concept of provenance can emphasize the reconstruction of a fonds when records have been moved, rearranged, and dispersed, this article draws attention to the ‘curative’ and ‘rehabilitative’ orientations of established notions of provenance. Put in conversation with disability studies scholarship, which critiques rehabilitating, curing, and restoring, this article outlines the theoretical scaffolding of a crip provenance: a disability-centered framework of resisting the desire to restore and instead meets records where they are …

An Ecophisiological Proposal To Manage Natural Grasslands: A Long Term Trial, F. L. F. De Quadros, D. Roberti, L. Marin, B. Kuinchtner, P. L. Nascimento

IGC Proceedings (1977-2023)

Natural grasslands on Southern Brazil comprise the so called “Rio de La Plata Grasslands” in South America. They are an important fodder source for ruminant pastoral systems and contribute to regional ecosystem services. Strength of these grasslands is its floristic diversity that poses a dilemma to farmers: how to choose management protocols that could be applied for hundreds of species. We propose to use a functional ecophysiological approach based on groups of grasses, the most abundant on aerial biomass of this natural grasslands. We clustered the most frequent grasses in two groups based on its leaf traits (leaf dry matter …

When The Natural Toxin Concentration In Crotalaria Increase In Japan?, M. Kaneko, N. Kato, I. Hattori

IGC Proceedings (1977-2023)

A green manure crop, sunn hemp (Crotalaria juncea L.), legumes, has a nematode-suppressive effect. It is also used as feed in India. However, it is not used as feed in Japan because it contains natural toxins: Pyrrolizidine alkaloids (PAs). PAs has the negative effects for not only livestock but also human health. The PAs, which are abundant in sunn hemp seeds and which are contained only slightly in leaves and stems. Therefore, if sunn hemp harvested before flowering, then no poison problem is posed by PAs. However, if it is harvested at the podding stage, a poisonous concentration of …

Accelerate Scaling Up Forage Intensification Using Novel Digital Extension Approach In Kenya, Donald M. G. Njarui, Mwangi Gatheru, Jessica M. Ndubi, Alice W. Murage, Anne W. Gichangi, Elias M. Gichangi, Udaya S. Nogothu

IGC Proceedings (1977-2023)

Wide scale adoption of diverse forages improves livestock productivity and farmers welfare. However, limited access to information and knowledge on forage production results in slow adoption in Kenya. There is need to enhance information and knowledge exchange among farming communities for efficient and effective adoption and decision-making. An inter-institutional pilot project was initiated in 2017 to scale-up forages in Kenya using a novel extension approach - the village knowledge centre (VKC). A VKC is an information and communication technology (ICT) digital platform-based linking farmers through smart phones and social media as a conduit for faster and effective information and knowledge. …

Evaluation Of Annual Companion Crops For The Establishment Of Perennial Forage Crops In Eastern Canada, Philippe Seguin, Sandrine St-Pierre-Lepage, Céline Georlette, Caroline Halde, Gaëtan F. Tremblay, Huguette Martel, Ayitre Akpakouma

IGC Proceedings (1977-2023)

The use of companion crops when establishing perennial forages is desirable as it often reduces weed growth and increases forage biomass in the seeding year. In eastern Canada, oat (Avena sativa L.) is the main species used as companion crop; although other species are used, they have not been systematically evaluated. A field study was established in 2019 at three sites in Québec, Canada, to contrast the use of six annual species as companion crops for the establishment of lucerne (Medicago sativa L.)-timothy (Phleum pratense L.) mixtures. Species evaluated include berseem clover (Trifolium alexandrinum L.), annual …

Variation Of Pollen Viability In Brachiaria Species And Opportunity In Increased Filled Seed Percentage, Weenaporn Juntasin, Sarayut Thaikua, Rattikan Poungkaew, Pichet Chanpeng, Sompon Waipanya, Yasuhiro Kawamoto

IGC Proceedings (1977-2023)

On pseudogamy process, pollen fertility affects endosperm development in Brachiaria apomictic hybrids. Also, it was well-known that self-incompatibility could affect the fertilization in cross pollination species. Evaluation of pollen viability and percentage of filled seed, and the demonstration of pollen induction technique for increasing filled seed percentage in Brachiaria spp. are presented in this paper. In the first experiment, 8 cultivars/breeding lines of Brachiaria, including 2 open-pollinated cultivars, an apomictic cultivar and 5 hybrids, were planted in randomized completely block design, with 3 replications at northeast Thailand. Each experimental unit consisted of 20 plants, 6 central plants were evaluated. …

Pollution — Bring The Field Into The Lab, Anna Von Mikecz, Andrea Scharf

Biological Sciences Faculty Research & Creative Works

Letter to the Editor

Age Specific Models To Capture The Change In Risk Factor Contribution By Age To Short Term Primary Ischemic Stroke Risk, Elizabeth Hunter, John D. Kelleher

Articles

Age is one of the most important risk factors when it comes to stroke risk prediction. However, including age as a risk factor in a stroke prediction model can give rise to a number of difficulties. Age often dominates the risk score, and also not all risk factors contribute proportionally to stroke risk by age. In this study we investigate a number of common stroke risk factors, using Framingham heart study data from the NHLBI Biologic Specimen and Data Repository Information Coordinating Center to determine if they appear to contribute proportionally by age to a stroke risk score. As we …

February 2022 Academic Affairs Minutes, University Of Nebraska At Kearney Academic Affairs Committee

Academic Affairs Committee Minutes

No abstract provided.

Is Title Vii An "Anti-Discrimination" Law?, Anuj C. Desai

University of Colorado Law Review Forum

No abstract provided.

The Minutes Of The Marshall University Staff Council Meeting, February 2022, Marshall University Staff Council

Staff Council Minutes

No abstract provided.

Temporal Trends Of Persistent Organic Pollutants In Sarasota Bay Common Bottlenose Dolphins (Tursiops Truncatus), John Kucklick, Ashley Boggs, Kevin Huncik, Amanda Moors, Elizabeth Davis, Gina Ylitalo, Mary Mcconnell, Christina Makris, Randall S. Wells

Scholarship and Professional Work – COPHS

No abstract provided.

Spatiotemporal Dynamics Of Clonal Selection And Diversification In Normal Endometrial Epithelium., Manako Yamaguchi, Hirofumi Nakaoka, Kazuaki Suda, Kosuke Yoshihara, Tatsuya Ishiguro, Nozomi Yachida, Kyota Saito, Haruka Ueda, Kentaro Sugino, Yutaro Mori, Kaoru Yamawaki, Ryo Tamura, Sundaramoorthy Revathidevi, Teiichi Motoyama, Kazuki Tainaka, Roel G W Verhaak, Ituro Inoue, Takayuki Enomoto

Faculty Research 2022

It has become evident that somatic mutations in cancer-associated genes accumulate in the normal endometrium, but spatiotemporal understanding of the evolution and expansion of mutant clones is limited. To elucidate the timing and mechanism of the clonal expansion of somatic mutations in cancer-associated genes in the normal endometrium, we sequence 1311 endometrial glands from 37 women. By collecting endometrial glands from different parts of the endometrium, we show that multiple glands with the same somatic mutations occupy substantial areas of the endometrium. We demonstrate that "rhizome structures", in which the basal glands run horizontally along the muscular layer and multiple …

Murine Model For Measuring Effects Of Humanized-Dosing Of Antibiotics On The Gut Microbiome., Shana Leopold, Kamilia Abdelraouf, David P Nicolau, Hanako Agresta, Jethro Johnson, Kathleen Teter, Wm Michael Dunne, David Broadwell, Alex Van Belkum, Lisa M Schechter, Erica J Sodergren, George M. Weinstock

Faculty Research 2022

There is a current need for enhancing our insight in the effects of antimicrobial treatment on the composition of human microbiota. Also, the spontaneous restoration of the microbiota after antimicrobial treatment requires better understanding. This is best addressed in well-defined animal models. We here present a model in which immune-competent or neutropenic mice were administered piperacillin-tazobactam (TZP) according to human treatment schedules. Before, during and after the TZP treatment, fecal specimens were longitudinally collected at established intervals over several weeks. Gut microbial taxonomic distribution and abundance were assessed through culture and molecular means during all periods. Non-targeted metabolomics analyses of …