Digital Commons Network^™

A Review On Antibacterial Activity Of Nanoparticles, Badr-Edine Sadoq, Mohammed Reda Britel, Adel Bouajaj, Ramzi Maâlej, Ahmed Touhami, Marwa Abid, Hanen Douiri, Fakhita Touhami, Amal Maurady

Physics & Astronomy Faculty Publications

The increasing resistance of bacteria to antibiotic agents is a main global public health problem. The use of nanoparticles is one of the promising ways to overcome microbial resistance to antimicrobial agents. Metal nanoparticles are increasingly used to target bacterial strains. Advances in nanotechnology, in particular the ability to synthesize nanoparticles of specific size and shape, are likely to lead to the development of new antibacterial agents. The antibacterial activities of nanoparticles are largely influenced by their sizes and large surface area/mass ratio. The antibacterial mechanisms of nanoparticles are poorly understood, but the currently accepted mechanisms include oxidative stress induction, …

The Vermont Family Based Approach In Primary Care Pediatrics: Effects On Children’S And Parents’ Emotional And Behavioral Problems And Parents’ Health-Related Quality Of Life, Masha Y. Ivanova, Allison Hall, Stanley Weinberger, Sara L. Buckingham, William E. Copeland, Phoenix Crockett, Justin Dainer-Best, Casey D'Alberto, Lauren Dewey, Deshan Foret, Maria Galano, Lisa Goodrich, Lindsay E. Holly, Nalini Lane, Maureen Leahey, Mathew Lerner, Jasime Marsh, Ellen Mcginnis, Melissa Paiva-Salisbury, Judith S. Shaw, Pamela Swift, Rebekah Tinker, James J. Hudziak

Psychology Faculty Research and Publications

This randomized controlled trial tested the Vermont Family Based Approach (VFBA) in primary care pediatrics. The VFBA is a model of healthcare delivery that shifts the focus from the individual to the family, emphasizes emotional and behavioral health, and uses evidence-based health promotion/prevention along with the treatment of emotional and behavioral problems. Participants were 81 families of 3–15-year-olds. For children, the VFBA was associated with greater reductions than the Control condition on the Child Behavior Checklist Emotionally Reactive, Withdrawn, Sleep Problems, Aggressive Behavior and Total Problems scales. For parents, the VFBA was associated with greater reductions than the Control condition …

When Planetary Cosmopolitanism Meets The Buddhist Ethic: Recycling, Karma And Popular Ecology In Singapore, Siew Ying Shee, Orlando Woods, Lily Kong

Research Collection College of Integrative Studies

By thinking with and through Buddhist cosmology, this paper explores the emergence of an ethical sensibility—what we call planetary cosmopolitanism—that is based on not just a spatially expanded ethic of care to ecological worlds, but also a temporally extended sense of justice to the future Earth. This transtemporal sense of ethical becoming reflects how the possibility of future ‘rebirth’ and accountability for past actions can motivate new ecological consciousness in the present. We forge these ideas through an empirical focus on popular Buddhist ecological practices in Singapore, where green recovery visions have primarily been driven by a secular and technocratic …

Why Do Farmers’ Cooperatives Fail In A Market Economy? Rediscovering Chayanov With The Chinese Experience, Zhanping Hu, Qian Forrest Zhang, John A. Donaldson

Research Collection School of Social Sciences

In The Theory of Peasant Cooperatives, Chayanov develops the theories of differential optima and vertical integration, which stress the vulnerability of peasant farming in capitalist markets, and argues that cooperatives can support smallholders only if they operate as ‘a cooperative movement’, are buttressed by a strong ‘cooperative culture’, and achieve ‘vertical integration’. Based on extensive fieldwork in China, we identify six major obstacles that explain the failure of most cooperatives. Chayanov’s arguments caution us to not only the vital importance of cooperatives to the resilience of peasant farming, but also the apparently insurmountable obstacles that cooperatives face in market economies.

Social Anhedonia, Communication And Marital Satisfaction In Newlywed Couples, Kenneth Tan, Amber M. Jarnecke, Susan C. South

Research Collection School of Social Sciences

Objective: Social anhedonia is associated with disinterest in social interactions and poor relationship functioning, yet little is known about the specific mechanisms underlying associations between social anhedonia and romantic relationship behaviors and satisfaction. We examined the links between social anhedonia, perceptions of conflict communication patterns, and marital satisfaction. Method: The current research examined the role of social anhedonia on marital quality and functioning longitudinally across a year in a sample of 100 newlywed couples using an actor-partner interdependence framework. Results: Social anhedonia was negatively associated with own and partner's marital satisfaction. It was also negatively associated with constructive communication and …

Cross-Group Differences In Age, Period, And Cohort Effects: A Bounding Approach To The Gender Wage Gap, Ohjae Gowen, Ethan Fosse, Christopher Winship

Research Collection School of Social Sciences

For decades, researchers have sought to understand the separate contributions of age, period, and cohort (APC) on a wide range of outcomes. However, a major challenge in these efforts is the linear dependence among the three time scales. Previous methods have been plagued by either arbitrary assumptions or extreme sensitivity to small variations in model specification. In this article, we present an alternative method that achieves partial identification by leveraging additional information about subpopulations (or strata) such as race, gender, and social class. Our first goal is to introduce the cross-strata linearized APC (CSL-APC) model, a re-parameterization of the traditional …

Broodmother: What The Lack Of Bathroom Breaks Reveals About Dehumanizing Teachers, Jonathan Michael Coker

Educational Leadership

This chapter asks, “What is it like to be a public school teacher in a school that doesn't allow for adequate bathroom breaks?” Using van Manen's interpretive phenomenological methodology, the authors analyzed interviews from nine participants to create an empathetic portrayal of teachers who have inadequate bathroom breaks. These experiences were then distilled into a series of insights through the concept of “broodmothering.” These broodmothers deny their own physical and mental needs in the interest of supervising children, and show a profession burdened by increasing parentification and dehumanization of teachers. After connecting this phenomenon to larger issues in the profession, …

Quantitative Multiplexed Imaging Technologies For Single-Cell Analysis To Assess Predictive Markers For Immunotherapy In Thoracic Immuno-Oncology: Promises And Challenges, Edwin Roger Parra, Marius Ilié, Ignacio I Wistuba, Paul Hofman

Faculty, Staff and Student Publications

The past decade has witnessed a revolution in cancer treatment by the shift from conventional drugs (chemotherapies) towards targeted molecular therapies and immune-based therapies, in particular the immune-checkpoint inhibitors (ICIs). These immunotherapies selectively release the host immune system against the tumour and have shown unprecedented durable remission for patients with cancers that were thought incurable such as advanced non-small cell lung cancer (aNSCLC). The prediction of therapy response is based since the first anti-PD-1/PD-L1 molecules FDA and EMA approvals on the level of PD-L1 tumour cells expression evaluated by immunohistochemistry, and recently more or less on tumour mutation burden in …

Clinical And Biological Significance Of Circulating Mirnas In Chronic Pancreatitis Patients Undergoing Total Pancreatectomy With Islet Autotransplantation, Srividya Vasu, Giovanna Saracino, Carly M Darden, Kenjiro Kumano, Yang Liu, Michael C Lawrence, Bashoo Naziruddin

Faculty, Staff and Student Publications

Background: Specific microRNAs (miRNAs) were elevated in chronic pancreatitis (CP) patients during islet infusion after total pancreatectomy (TPIAT). We aimed to identify circulating miRNA signatures of pancreatic damage, predict miRNA-mRNA networks to identify potential links to CP pathogenesis and identify islet isolation and transplantation functional outcomes.

Methods: Small RNA sequencing was performed to identify distinct circulating miRNA signatures in CP. Plasma miRNAs were measured using miRCURY LNA SYBR green quantitative real-time polymerase chain reaction assays. Correlation analyses were performed using R software. The miRNA target and disease interactions were determined using miRNet and the miRNA enrichment and annotation tool.

Results: …

Help-Seeking Behavior Among Resettled Refugee, Mahfoudha Sid’Elemine

Graduate Program in International Studies Theses & Dissertations

The refugee resettlement process is a huge part of refugees’ journeys in their new countries that has a lasting impact on their long-term integration. The literature highlights the importance of trust for successful refugee resettlement. This dissertation, however, challenges assumptions about the primary importance of “trust” and argues that the importance of trust is relative and can depend on the nature of the resettlement program and the perspective and background of refugees. In other words, there is not a one-size-fits-all of trust building in the context of resettlement. For certain resettlement programs trust building can be counterproductive when resources are …

Complex Dynamics Of Contention: Towards A Generative Model Of Social Dissent, Travis Holmes

Graduate Program in International Studies Theses & Dissertations

In 2003, McAdam et al. released their seminal work Dynamics of Contention as a challenge to scholars within the field of contentious politics. In it, they claimed that the field required a refocusing from static and topical arguments splintered into various disciplines, to a more unified research agenda in search of causal mechanisms. In the spirit of their work, this study seeks to revisit this original critique considering more recent scholarship in the fields of Complexity and Systems Sciences along with certain technological advances in the field of computational social science, namely, the Agent-Based Model (ABM). In addition, both computational …

Dynamics Of The Dynll1-Mre11 Complex Regulate Dna End Resection And Recruitment Of Shieldin To Dsbs, Michelle L Swift, Rui Zhou, Aleem Syed, Lisa A Moreau, Bartłomiej Tomasik, John A Tainer, Panagiotis A Konstantinopoulos, Alan D D'Andrea, Yizhou Joseph He, Dipanjan Chowdhury

Faculty, Staff and Student Publications

The extent and efficacy of DNA end resection at DNA double-strand breaks (DSB) determine the repair pathway choice. Here we describe how the 53BP1-associated protein DYNLL1 works in tandem with the Shieldin complex to protect DNA ends. DYNLL1 is recruited to DSBs by 53BP1, where it limits end resection by binding and disrupting the MRE11 dimer. The Shieldin complex is recruited to a fraction of 53BP1-positive DSBs hours after DYNLL1, predominantly in G1 cells. Shieldin localization to DSBs depends on MRE11 activity and is regulated by the interaction of DYNLL1 with MRE11. BRCA1-deficient cells rendered resistant to PARP inhibitors by …

Comparisons Of Medical Cost Trajectories Between Non-Hispanic Black And Non-Hispanic White Patients With Newly Diagnosed Localized Prostate Cancer, Yu Liu, Shikun Wang, Liang Li, Ying Xu, Yu Shen, Ya-Chen Tina Shih

Faculty, Staff and Student Publications

Objectives: This study applied a recently developed statistical method to compare the mean cost trajectories between non-Hispanic White (NHW) and non-Hispanic Black (NHB) patients with localized prostate cancer conditioning on patients' survival.

Methods: In this observational study, we modeled cost trajectories of NHW and NHB patients with localized prostate cancer for 3 survival durations: 24, 48, and 72 months. We also compared the cost trajectories between NHW and NHB, stratified by comorbidities scores.

Results: We find that the mean cost trajectories of NHB were significantly higher than the trajectories of NHW in the last 12 months before death, regardless of …

Preclinical Development Of 1b7/Cd3, A Novel Anti-Tslpr Bispecific Antibody That Targets Crlf2-Rearranged Ph-Like B-All, Ze Tian, Chunhua Shi, Guojun Yang, Jason K Allen, Qing Shi, Amin Al-Shami, Jill Wardell Olson, Melinda G Smith, Qing Chang, Jasbir Kaur, Junping You, Timothy E Lofton, Michelle A Gonzalez, Qi Zhang, Dongxing Zha, Sarah K Tasian, Nitin Jain, Marina Y Konopleva, Timothy Heffernan, Jeffrey J Molldrem

Faculty, Staff and Student Publications

Patients harboring CRLF2-rearranged B-lineage acute lymphocytic leukemia (B-ALL) face a 5-year survival rate as low as 20%. While significant gains have been made to position targeted therapies for B-ALL treatment, continued efforts are needed to develop therapeutic options with improved duration of response. Here, first we have demonstrated that patients with CRLF2-rearranged Ph-like ALL harbor elevated thymic stromal lymphopoietin receptor (TSLPR) expression, which is comparable with CD19. Then we present and evaluate the anti-tumor characteristics of 1B7/CD3, a novel CD3-redirecting bispecific antibody (BsAb) that co-targets TSLPR. In vitro, 1B7/CD3 exhibits optimal binding to both human and cynomolgus CD3 and TSLPR. …

Nuclear Smooth Muscle Α-Actin Participates In Vascular Smooth Muscle Cell Differentiation, Callie S Kwartler, Albert J Pedroza, Anita Kaw, Pujun Guan, Shuangtao Ma, Xue-Yan Duan, Caroline Kernell, Charis Wang, Jose Emiliano Esparza Pinelo, Mikayla S Borthwick Bowen, Jiyuan Chen, Yuan Zhong, Sanjay Sinha, Xuetong Shen, Michael P Fischbein, Dianna M Milewicz

Faculty, Staff and Student Publications

Missense variants throughout ACTA2, encoding smooth muscle α-actin (αSMA), predispose to adult-onset thoracic aortic disease, but variants disrupting arginine 179 (R179) lead to Smooth Muscle Dysfunction Syndrome (SMDS) characterized by diverse childhood-onset vascular diseases. Here we show that αSMA localizes to the nucleus in wildtype (WT) smooth muscle cells (SMCs), enriches in the nucleus with SMC differentiation, and associates with chromatin remodeling complexes and SMC contractile gene promotors. The ACTA2 p.R179 αSMA variant shows decreased nuclear localization. Primary SMCs from Acta2SMC-R179C/+ mice are less differentiated than WT SMCs in vitro and in vivo and have global changes in …

Phenotype And Fate Of Liver-Resident Cd8 T Cells During Acute And Chronic Hepacivirus Infection, Piyush Dravid, Satyapramod Murthy, Zayed Attia, Cole Cassady, Rahul Chandra, Sheetal Trivedi, Ashish Vyas, John Gridley, Brantley Holland, Anuradha Kumari, Arash Grakoui, John M Cullen, Christopher M Walker, Himanshu Sharma, Amit Kapoor

Faculty, Staff and Student Publications

Immune correlates of hepatitis C virus (HCV) clearance and control remain poorly defined due to the lack of an informative animal model. We recently described acute and chronic rodent HCV-like virus (RHV) infections in lab mice. Here, we developed MHC class I and class II tetramers to characterize the serial changes in RHV-specific CD8 and CD4 T cells during acute and chronic infection in C57BL/6J mice. RHV infection induced rapid expansion of T cells targeting viral structural and nonstructural proteins. After virus clearance, the virus-specific T cells transitioned from effectors to long-lived liver-resident memory T cells (TRM). The effector and …

Single-Cell Dissection Of Tumor Microenvironmental Response And Resistance To Cancer Therapy, Yikai Luo, Han Liang

Faculty, Staff and Student Publications

Cancer treatment strategies have evolved significantly over the years, with chemotherapy, targeted therapy, and immunotherapy as major pillars. Each modality leads to unique treatment outcomes by interacting with the tumor microenvironment (TME), which imposes a fundamental selective pressure on cancer progression. The advent of single-cell profiling technologies has revolutionized our understanding of the intricate and heterogeneous nature of the TME at an unprecedented resolution. This review delves into the commonalities and differential manifestations of how cancer therapies reshape the microenvironment in diverse cancer types. We highlight how groundbreaking immune checkpoint blockade (ICB) strategies alone or in combination with tumor-targeting treatments …

Local Continual Reassessment Methods For Dose Finding And Optimization In Drug-Combination Trials, Jingyi Zhang, Fangrong Yan, Nolan A Wages, Ruitao Lin

Faculty, Staff and Student Publications

Due to the limited sample size and large dose exploration space, obtaining a desirable dose combination is a challenging task in the early development of combination treatments for cancer patients. Most existing designs for optimizing the dose combination are model-based, requiring significant efforts to elicit parameters or prior distributions. Model-based designs also rely on intensive model calibration and may yield unstable performance in the case of model misspecification or sparse data. We propose to employ local, underparameterized models for dose exploration to reduce the hurdle of model calibration and enhance the design robustness. Building upon the framework of the partial …

Evaluating Biotic And Abiotic Controls On Plant Stomatal Conductance Using Mathematical Models, Kenneth J. Davidson

Electronic Dissertations and Theses, 2010-current

Vegetation acts as a substantial link between the geosphere and atmosphere, mediating transpiration (E), and carbon assimilation (A). At the center of this process lie stomata, small pores which actively regulate the rate of CO2 and H2O diffusion between vegetation and the environment. This process, known as stomatal conductance (gsw) helps set the upper limit on E, and through stomatal limitation on A, is a strong determinant of net primary productivity. A series of mathematical models have been developed to predict the sensitivity of gsw to environmental factors, which have been incorporated into land surface models (LSMs). Despite the clear …

Genome-Wide Association Study And Genomic Prediction Of Fusarium Wilt Resistance In Common Bean Core Collection, Kenani Chiwina, Haizheng Xiong, Gehendra Bhattarai, Ryan Dickson, Theresa Makawa Phiri, Yilin Chen, Ibtisam Alatawi, Derek Dean, Neelendra K. Joshi, Yuyan Chen, Awais Riaz, Paul Gepts, Mark Brick, Patrick Byrne, Howard Schwartz, James B. Ogg, Kristen Otto, Amy Fall, Jeremy Gilbert, Ainong Shi

Horticulture Faculty Publications and Presentations

The common bean (Phaseolus vulgaris L.) is a globally cultivated leguminous crop. Fusarium wilt (FW), caused by Fusarium oxysporum f. sp. phaseoli (Fop), is a significant disease leading to substantial yield loss in common beans. Disease-resistant cultivars are recommended to counteract this. The objective of this investigation was to identify single nucleotide polymorphism (SNP) markers associated with FW resistance and to pinpoint potential resistant common bean accessions within a core collection, utilizing a panel of 157 accessions through the Genome-wide association study (GWAS) approach with TASSEL 5 and GAPIT 3. Phenotypes for Fop race 1 and race …

Conservation Agriculture Assists Smallholder Farmers And Their Agroecosystem In The Democratic Republic Of The Congo, Willy Mulimbi, Kristofor R. Brye, Lawton L. Nalley, Damas R. Birindwa

Agricultural Economics and Agribusiness Faculty Publications and Presentations

onservation agriculture, a sustainable farming practice combining no or minimum soil disturbance, crop diversification, and soil cover, can benefit humans and the biophysical environment. However, less than half of Sub-Saharan African countries implement conservation agriculture, and the majority of smallholder farmers are using unsustainable practices. Research and field trials on conservation agriculture adaptation to soil and agroecological conditions are vital for increasing adoption of conservation agriculture in Sub-Saharan Africa. The objectives of this study were to (i) evaluate the change in soil chemical properties and soil biology from implementation of conservation agriculture, (ii) assess cowpea (Vigna unguiculata L. Walp.) …

Psychology 2023 Apr Self-Study & Documents, Unm Academic Program Review

UNM Academic Program Review

UNM Department of Psychology self-study & review team report for Fall 2023, fulfilling requirements of Higher Learning Commission.

State: The Magazine Of Illinois State University, Fall 2023, Illinois State University, University Marketing And Communications

State: The Magazine of Illinois State University (1999-present)

Illinois State University alumni magazine

Development Of Pullulan/Chitosan/Salvianolic Acid Ternary Fibrous Membranes And Their Potential For Chemotherapeutic Applications, Salahuddin Ahmed, Megan Keniry, Victoria Padilla, Narcedalia Anaya-Barbosa, Md Noushad Javed, Robert Gilkerson, Kithzia Gomez, Acharan S. Narula, Karen Lozano

Mechanical Engineering Faculty Publications

This study investigates the feasibility of centrifugal spinning for producing fibrous membranes containing pullulan, chitosan, and danshen extract. The danshen extract is composed of 20 wt% salvianolic acid B (SA). Citric acid was added to the mixture as a crosslinking agent to promote its use in the aqueous medium. The influence of the danshen concentration (25 wt% and 33 wt%) on fiber morphology, thermal behavior, and the biochemical effect was analyzed. Developed fiber-based membranes consist of long, continuous, and uniform fibers with a sparse scattering of beads. Fiber diameter analysis shows values ranging from 384 ± 123 nm to 644 …

Matter Of Will Of Ratcliff And The Not-So-Harmless Error: A Call To Change Mississippi’S Approach To Will Formalities, Kelsi Baldwin

MC Law Review

A will provides a mechanism to dispose of property at death. But costly litigation—or worse, a will’s invalidation—often thwart this purpose. The law of probate is state-specific, which leaves jurisdictions with the burden of ensuring that their laws promote rather than defeat the purpose of probate—to honor the testator’s intent. Mississippi attempts to recognize this purpose by requiring strict compliance with the statutory requirements for creating a will. This “better safe than sorry” approach errs on the side of invalidity with the hope that denying a non-compliant instrument for probate will prevent fraud and other wrongdoing.

Despite its intention, Mississippi’s …

Integrating Clinical Cancer And Ptm Proteomics Data Identifies A Mechanism Of Ack1 Kinase Activation, Eranga R. Balasooriya, Deshan Madhusanka, Tania P. López-Palacios, Riley J. Eastmond, Dasun Jayatunge, Jake J. Owen, Jack S. Gashler, Christina M. Egbert, Chanaka Bulathsinghalage, Lu Liu, Stephen R. Piccolo, Joshua L. Andersen

Faculty Publications

Beyond the most common oncogenes activated by mutation (mut-drivers), there likely exists a variety of low-frequency mut-drivers, each of which is a possible frontier for targeted therapy. To identify new and understudied mut-drivers, we developed a machine learning (ML) model that integrates curated clinical cancer data and posttranslational modification (PTM) proteomics databases. We applied the approach to 62,746 patient cancers spanning 84 cancer types and predicted 3,964 oncogenic mutations across 1,148 genes, many of which disrupt PTMs of known and unknown function. The list of putative mut-drivers includes established drivers and others with poorly understood roles in cancer. This ML …

Energy, Passivity, And Morphological Capacities, Pablo Borja, Cosimo Della Santina, Azita Dabiri

School of Engineering, Computing and Mathematics

The morphology of a system may be exploited to reduce the controller workload or to perform specific tasks, e.g., locomotion processes. However, understanding and characterizing these capacities in terms of physical quantities remains an open problem. In this paper, we pose the question: are energy and dissipation useful physical quantities to characterize morphological capacities? First, to illustrate the relevance of this question, we present some basic concepts of passivity theory that are useful for carrying out an energy-based analysis of the behavior of dynamical systems. Then, we analyze the behavior of a mechanical system, and we show how its morphology …

Calculation Of Atp Production Rates Using The Seahorse Xf Analyzer, Brandon R. Desousa, Kristen K.O. Kim, Anthony E. Jones, Andréa B. Ball, Wei Y. Hsieh, Pamela Swain, Danielle H. Morrow, Alexandra J. Brownstein, David A. Ferrick, Orian S. Shirihai, Andrew Neilson, David A. Nathanson, George W. Rogers, Brian P. Dranka, Anne N. Murphy, Charles Affourtit, Steven J. Bensinger, Linsey Stiles, Natalia Romero, Ajit S. Divakaruni

School of Biomedical Sciences

Oxidative phosphorylation and glycolysis are the dominant ATP-generating pathways in mammalian metabolism. The balance between these two pathways is often shifted to execute cell-specific functions in response to stimuli that promote activation, proliferation, or differentiation. However, measurement of these metabolic switches has remained mostly qualitative, making it difficult to discriminate between healthy, physiological changes in energy transduction or compensatory responses due to metabolic dysfunction. We therefore present a broadly applicable method to calculate ATP production rates from oxidative phosphorylation and glycolysis using Seahorse XF Analyzer data and empirical conversion factors. We quantify the bioenergetic changes observed during macrophage polarization as …

Soil Adsorption Of Heavy Metals To Protect Groundwater Near Refinery Wastewater Discharge Points, Hamid A. Al-Falahi, Ferdous A. Jabir, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

This study presents a strategy to manage the discharge of wastewater, which is either partially treated or untreated, to safeguard the groundwater reserves in the region. a case study involving Ad Diwaniyah refinery wastewater disposal into an adjacent desert was utilized to evaluate the influence of soil adsorption on the attenuation of Pb, Cu, and Cd ion concentrations. Investigation of competitive adsorption of heavy metal ions (Pb2+, Cu2+, and Cd2+) in Dolomite-Limestone soil was conducted through batch and column methods. the adsorption behavior of the bivalent metal cations was observed to be pH dependent. However, the competitive extraction of the …

Catching Elusive Depression Via Facial Micro-Expression Recognition, Xiaohui Chen, Tony Tie (T.) Luo

Computer Science Faculty Research & Creative Works

Depression is a common mental health disorder that can cause consequential symptoms with continuously depressed mood that leads to emotional distress. One category of depression is Concealed Depression, where patients intentionally or unintentionally hide their genuine emotions through exterior optimism, thereby complicating and delaying diagnosis and treatment and leading to unexpected suicides. In this article, we propose to diagnose concealed depression by using facial micro-expressions (FMEs) to detect and recognize underlying true emotions. However, the extremely low intensity and subtle nature of FMEs make their recognition a tough task. We propose a facial landmark-based Region-of-Interest (ROI) approach to address the …