Digital Commons Network^™

Preclinical Development Of 1b7/Cd3, A Novel Anti-Tslpr Bispecific Antibody That Targets Crlf2-Rearranged Ph-Like B-All, Ze Tian, Chunhua Shi, Guojun Yang, Jason K Allen, Qing Shi, Amin Al-Shami, Jill Wardell Olson, Melinda G Smith, Qing Chang, Jasbir Kaur, Junping You, Timothy E Lofton, Michelle A Gonzalez, Qi Zhang, Dongxing Zha, Sarah K Tasian, Nitin Jain, Marina Y Konopleva, Timothy Heffernan, Jeffrey J Molldrem

Faculty, Staff and Student Publications

Patients harboring CRLF2-rearranged B-lineage acute lymphocytic leukemia (B-ALL) face a 5-year survival rate as low as 20%. While significant gains have been made to position targeted therapies for B-ALL treatment, continued efforts are needed to develop therapeutic options with improved duration of response. Here, first we have demonstrated that patients with CRLF2-rearranged Ph-like ALL harbor elevated thymic stromal lymphopoietin receptor (TSLPR) expression, which is comparable with CD19. Then we present and evaluate the anti-tumor characteristics of 1B7/CD3, a novel CD3-redirecting bispecific antibody (BsAb) that co-targets TSLPR. In vitro, 1B7/CD3 exhibits optimal binding to both human and cynomolgus CD3 and TSLPR. …

Nuclear Smooth Muscle Α-Actin Participates In Vascular Smooth Muscle Cell Differentiation, Callie S Kwartler, Albert J Pedroza, Anita Kaw, Pujun Guan, Shuangtao Ma, Xue-Yan Duan, Caroline Kernell, Charis Wang, Jose Emiliano Esparza Pinelo, Mikayla S Borthwick Bowen, Jiyuan Chen, Yuan Zhong, Sanjay Sinha, Xuetong Shen, Michael P Fischbein, Dianna M Milewicz

Faculty, Staff and Student Publications

Missense variants throughout ACTA2, encoding smooth muscle α-actin (αSMA), predispose to adult-onset thoracic aortic disease, but variants disrupting arginine 179 (R179) lead to Smooth Muscle Dysfunction Syndrome (SMDS) characterized by diverse childhood-onset vascular diseases. Here we show that αSMA localizes to the nucleus in wildtype (WT) smooth muscle cells (SMCs), enriches in the nucleus with SMC differentiation, and associates with chromatin remodeling complexes and SMC contractile gene promotors. The ACTA2 p.R179 αSMA variant shows decreased nuclear localization. Primary SMCs from Acta2SMC-R179C/+ mice are less differentiated than WT SMCs in vitro and in vivo and have global changes in …

Phenotype And Fate Of Liver-Resident Cd8 T Cells During Acute And Chronic Hepacivirus Infection, Piyush Dravid, Satyapramod Murthy, Zayed Attia, Cole Cassady, Rahul Chandra, Sheetal Trivedi, Ashish Vyas, John Gridley, Brantley Holland, Anuradha Kumari, Arash Grakoui, John M Cullen, Christopher M Walker, Himanshu Sharma, Amit Kapoor

Faculty, Staff and Student Publications

Immune correlates of hepatitis C virus (HCV) clearance and control remain poorly defined due to the lack of an informative animal model. We recently described acute and chronic rodent HCV-like virus (RHV) infections in lab mice. Here, we developed MHC class I and class II tetramers to characterize the serial changes in RHV-specific CD8 and CD4 T cells during acute and chronic infection in C57BL/6J mice. RHV infection induced rapid expansion of T cells targeting viral structural and nonstructural proteins. After virus clearance, the virus-specific T cells transitioned from effectors to long-lived liver-resident memory T cells (TRM). The effector and …

Single-Cell Dissection Of Tumor Microenvironmental Response And Resistance To Cancer Therapy, Yikai Luo, Han Liang

Faculty, Staff and Student Publications

Cancer treatment strategies have evolved significantly over the years, with chemotherapy, targeted therapy, and immunotherapy as major pillars. Each modality leads to unique treatment outcomes by interacting with the tumor microenvironment (TME), which imposes a fundamental selective pressure on cancer progression. The advent of single-cell profiling technologies has revolutionized our understanding of the intricate and heterogeneous nature of the TME at an unprecedented resolution. This review delves into the commonalities and differential manifestations of how cancer therapies reshape the microenvironment in diverse cancer types. We highlight how groundbreaking immune checkpoint blockade (ICB) strategies alone or in combination with tumor-targeting treatments …

Local Continual Reassessment Methods For Dose Finding And Optimization In Drug-Combination Trials, Jingyi Zhang, Fangrong Yan, Nolan A Wages, Ruitao Lin

Faculty, Staff and Student Publications

Due to the limited sample size and large dose exploration space, obtaining a desirable dose combination is a challenging task in the early development of combination treatments for cancer patients. Most existing designs for optimizing the dose combination are model-based, requiring significant efforts to elicit parameters or prior distributions. Model-based designs also rely on intensive model calibration and may yield unstable performance in the case of model misspecification or sparse data. We propose to employ local, underparameterized models for dose exploration to reduce the hurdle of model calibration and enhance the design robustness. Building upon the framework of the partial …

Evaluating Biotic And Abiotic Controls On Plant Stomatal Conductance Using Mathematical Models, Kenneth J. Davidson

Electronic Dissertations and Theses, 2010-current

Vegetation acts as a substantial link between the geosphere and atmosphere, mediating transpiration (E), and carbon assimilation (A). At the center of this process lie stomata, small pores which actively regulate the rate of CO2 and H2O diffusion between vegetation and the environment. This process, known as stomatal conductance (gsw) helps set the upper limit on E, and through stomatal limitation on A, is a strong determinant of net primary productivity. A series of mathematical models have been developed to predict the sensitivity of gsw to environmental factors, which have been incorporated into land surface models (LSMs). Despite the clear …

Genome-Wide Association Study And Genomic Prediction Of Fusarium Wilt Resistance In Common Bean Core Collection, Kenani Chiwina, Haizheng Xiong, Gehendra Bhattarai, Ryan Dickson, Theresa Makawa Phiri, Yilin Chen, Ibtisam Alatawi, Derek Dean, Neelendra K. Joshi, Yuyan Chen, Awais Riaz, Paul Gepts, Mark Brick, Patrick Byrne, Howard Schwartz, James B. Ogg, Kristen Otto, Amy Fall, Jeremy Gilbert, Ainong Shi

Horticulture Faculty Publications and Presentations

The common bean (Phaseolus vulgaris L.) is a globally cultivated leguminous crop. Fusarium wilt (FW), caused by Fusarium oxysporum f. sp. phaseoli (Fop), is a significant disease leading to substantial yield loss in common beans. Disease-resistant cultivars are recommended to counteract this. The objective of this investigation was to identify single nucleotide polymorphism (SNP) markers associated with FW resistance and to pinpoint potential resistant common bean accessions within a core collection, utilizing a panel of 157 accessions through the Genome-wide association study (GWAS) approach with TASSEL 5 and GAPIT 3. Phenotypes for Fop race 1 and race …

Conservation Agriculture Assists Smallholder Farmers And Their Agroecosystem In The Democratic Republic Of The Congo, Willy Mulimbi, Kristofor R. Brye, Lawton L. Nalley, Damas R. Birindwa

Agricultural Economics and Agribusiness Faculty Publications and Presentations

onservation agriculture, a sustainable farming practice combining no or minimum soil disturbance, crop diversification, and soil cover, can benefit humans and the biophysical environment. However, less than half of Sub-Saharan African countries implement conservation agriculture, and the majority of smallholder farmers are using unsustainable practices. Research and field trials on conservation agriculture adaptation to soil and agroecological conditions are vital for increasing adoption of conservation agriculture in Sub-Saharan Africa. The objectives of this study were to (i) evaluate the change in soil chemical properties and soil biology from implementation of conservation agriculture, (ii) assess cowpea (Vigna unguiculata L. Walp.) …

Allogeneic Hematopoietic Cell Transplantation Improves Outcome In Myelodysplastic Syndrome Across High-Risk Genetic Subgroups: Genetic Analysis Of The Blood And Marrow Transplant Clinical Trials Network 1102 Study, Jurjen Versluis, Peter Westervelt, Et Al.

2020-Current year OA Pubs

PURPOSE: Allogeneic hematopoietic cell transplantation (HCT) in patients with myelodysplastic syndrome (MDS) improves overall survival (OS). We evaluated the impact of MDS genetics on the benefit of HCT in a biological assignment (donor

METHODS: We performed targeted sequencing in 309 patients age 50-75 years with International Prognostic Scoring System (IPSS) intermediate-2 or high-risk MDS, enrolled in the Blood and Marrow Transplant Clinical Trials Network 1102 study and assessed the association of gene mutations with OS. Patients with

RESULTS: The distribution of gene mutations was similar in the donor and no donor arms, with

CONCLUSION: HCT improved OS compared with non-HCT …

Psychology 2023 Apr Self-Study & Documents, Unm Academic Program Review

UNM Academic Program Review

UNM Department of Psychology self-study & review team report for Fall 2023, fulfilling requirements of Higher Learning Commission.

State: The Magazine Of Illinois State University, Fall 2023, Illinois State University, University Marketing And Communications

State: The Magazine of Illinois State University (1999-present)

Illinois State University alumni magazine

Development Of Pullulan/Chitosan/Salvianolic Acid Ternary Fibrous Membranes And Their Potential For Chemotherapeutic Applications, Salahuddin Ahmed, Megan Keniry, Victoria Padilla, Narcedalia Anaya-Barbosa, Md Noushad Javed, Robert Gilkerson, Kithzia Gomez, Acharan S. Narula, Karen Lozano

Mechanical Engineering Faculty Publications

This study investigates the feasibility of centrifugal spinning for producing fibrous membranes containing pullulan, chitosan, and danshen extract. The danshen extract is composed of 20 wt% salvianolic acid B (SA). Citric acid was added to the mixture as a crosslinking agent to promote its use in the aqueous medium. The influence of the danshen concentration (25 wt% and 33 wt%) on fiber morphology, thermal behavior, and the biochemical effect was analyzed. Developed fiber-based membranes consist of long, continuous, and uniform fibers with a sparse scattering of beads. Fiber diameter analysis shows values ranging from 384 ± 123 nm to 644 …

Matter Of Will Of Ratcliff And The Not-So-Harmless Error: A Call To Change Mississippi’S Approach To Will Formalities, Kelsi Baldwin

MC Law Review

A will provides a mechanism to dispose of property at death. But costly litigation—or worse, a will’s invalidation—often thwart this purpose. The law of probate is state-specific, which leaves jurisdictions with the burden of ensuring that their laws promote rather than defeat the purpose of probate—to honor the testator’s intent. Mississippi attempts to recognize this purpose by requiring strict compliance with the statutory requirements for creating a will. This “better safe than sorry” approach errs on the side of invalidity with the hope that denying a non-compliant instrument for probate will prevent fraud and other wrongdoing.

Despite its intention, Mississippi’s …

A Systematic Review And Meta-Analysis Of Resting-State Fmri In Anxiety Disorders: Need For Data Sharing To Move The Field Forward, André Zugman, Laura Jett, Chase Antonacci, Anderson M. Winkler, Daniel Samuel Pine

School of Medicine Publications

Highlights

• There are numerous studies investigating rs-fMRI and anxiety; however, the methodologies are inconsistent.

• Anxiety patients show hypoconnectivity between the amygdala and the medial prefrontal cortex.

• This finding is not robust against possible publication bias. Thus, no strong findings manifest in available literature.

• More studies are needed with standardized methods and larger sample sizes.

Abstract

Anxiety disorders are among the most prevalent psychiatric disorders. Neuroimaging findings remain uncertain, and resting state functional magnetic resonance (rs-fMRI) connectivity is of particular interest since it is a scalable functional imaging modality. Given heterogeneous past findings for rs-fMRI in anxious individuals, we characterize patterns …

Autophagy Modulation And Its Implications On Glioblastoma Treatment, Johnny Chen, Andrea Salinas Rodriguez, Maximiliano Arath Morales, Xiaoqian Fang

School of Medicine Publications

Autophagy is a vital cellular process that functions to degrade and recycle damaged organelles into basic metabolites. This allows a cell to adapt to a diverse range of challenging conditions. Autophagy assists in maintaining homeostasis, and it is tightly regulated by the cell. The disruption of autophagy has been associated with many diseases, such as neurodegenerative disorders and cancer. This review will center its discussion on providing an in-depth analysis of the current molecular understanding of autophagy and its relevance to brain tumors. We will delve into the current literature regarding the role of autophagy in glioma pathogenesis by exploring …

Integrating Clinical Cancer And Ptm Proteomics Data Identifies A Mechanism Of Ack1 Kinase Activation, Eranga R. Balasooriya, Deshan Madhusanka, Tania P. López-Palacios, Riley J. Eastmond, Dasun Jayatunge, Jake J. Owen, Jack S. Gashler, Christina M. Egbert, Chanaka Bulathsinghalage, Lu Liu, Stephen R. Piccolo, Joshua L. Andersen

Faculty Publications

Beyond the most common oncogenes activated by mutation (mut-drivers), there likely exists a variety of low-frequency mut-drivers, each of which is a possible frontier for targeted therapy. To identify new and understudied mut-drivers, we developed a machine learning (ML) model that integrates curated clinical cancer data and posttranslational modification (PTM) proteomics databases. We applied the approach to 62,746 patient cancers spanning 84 cancer types and predicted 3,964 oncogenic mutations across 1,148 genes, many of which disrupt PTMs of known and unknown function. The list of putative mut-drivers includes established drivers and others with poorly understood roles in cancer. This ML …

Energy, Passivity, And Morphological Capacities, Pablo Borja, Cosimo Della Santina, Azita Dabiri

School of Engineering, Computing and Mathematics

The morphology of a system may be exploited to reduce the controller workload or to perform specific tasks, e.g., locomotion processes. However, understanding and characterizing these capacities in terms of physical quantities remains an open problem. In this paper, we pose the question: are energy and dissipation useful physical quantities to characterize morphological capacities? First, to illustrate the relevance of this question, we present some basic concepts of passivity theory that are useful for carrying out an energy-based analysis of the behavior of dynamical systems. Then, we analyze the behavior of a mechanical system, and we show how its morphology …

Calculation Of Atp Production Rates Using The Seahorse Xf Analyzer, Brandon R. Desousa, Kristen K.O. Kim, Anthony E. Jones, Andréa B. Ball, Wei Y. Hsieh, Pamela Swain, Danielle H. Morrow, Alexandra J. Brownstein, David A. Ferrick, Orian S. Shirihai, Andrew Neilson, David A. Nathanson, George W. Rogers, Brian P. Dranka, Anne N. Murphy, Charles Affourtit, Steven J. Bensinger, Linsey Stiles, Natalia Romero, Ajit S. Divakaruni

School of Biomedical Sciences

Oxidative phosphorylation and glycolysis are the dominant ATP-generating pathways in mammalian metabolism. The balance between these two pathways is often shifted to execute cell-specific functions in response to stimuli that promote activation, proliferation, or differentiation. However, measurement of these metabolic switches has remained mostly qualitative, making it difficult to discriminate between healthy, physiological changes in energy transduction or compensatory responses due to metabolic dysfunction. We therefore present a broadly applicable method to calculate ATP production rates from oxidative phosphorylation and glycolysis using Seahorse XF Analyzer data and empirical conversion factors. We quantify the bioenergetic changes observed during macrophage polarization as …

Soil Adsorption Of Heavy Metals To Protect Groundwater Near Refinery Wastewater Discharge Points, Hamid A. Al-Falahi, Ferdous A. Jabir, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

This study presents a strategy to manage the discharge of wastewater, which is either partially treated or untreated, to safeguard the groundwater reserves in the region. a case study involving Ad Diwaniyah refinery wastewater disposal into an adjacent desert was utilized to evaluate the influence of soil adsorption on the attenuation of Pb, Cu, and Cd ion concentrations. Investigation of competitive adsorption of heavy metal ions (Pb2+, Cu2+, and Cd2+) in Dolomite-Limestone soil was conducted through batch and column methods. the adsorption behavior of the bivalent metal cations was observed to be pH dependent. However, the competitive extraction of the …

Catching Elusive Depression Via Facial Micro-Expression Recognition, Xiaohui Chen, Tony Tie (T.) Luo

Computer Science Faculty Research & Creative Works

Depression is a common mental health disorder that can cause consequential symptoms with continuously depressed mood that leads to emotional distress. One category of depression is Concealed Depression, where patients intentionally or unintentionally hide their genuine emotions through exterior optimism, thereby complicating and delaying diagnosis and treatment and leading to unexpected suicides. In this article, we propose to diagnose concealed depression by using facial micro-expressions (FMEs) to detect and recognize underlying true emotions. However, the extremely low intensity and subtle nature of FMEs make their recognition a tough task. We propose a facial landmark-based Region-of-Interest (ROI) approach to address the …

Combination Therapy Of Triple-Negative Breast Cancer With Paclitaxel And Ambrisentan, A Selective Antagonist Of The Endothelin Type A Receptor, Inhibits Tumor Growth And Metastasis, Farah Nasser Almahrook

Theses

Cancer treatment is a big challenge especially when associated with metastasis. Moreover, the importance of using combination therapy is increasing, especially for highly invasive, drug-resistant cancers, such as triple-negative breast cancer (TNBC). Ambrisentan, a selective antagonist of the endothelin type A receptor (ETAR) was previously shown to reduce metastasis of TNBC in a preclinical tumor model when used prophylactically. In the current study, we investigated the therapeutic potential of Ambrisentan, given as monotherapy or in combination with Paclitaxel, on TNBC. The data show that oral dosing with Ambrisentan led to a significant retardation in tumor growth and metastasis. Moreover, combining …

Identification Of A Potential Pr50gag Binding Site Critical For Selective Encapsidation Of Feline Immunodeficiency Virus (Fiv) Genomic Rna, Anjana Krishnan

Dissertations

Feline immunodeficiency virus (FIV) is an important lentivirus that causes acquired immunodeficiency syndrome (AIDS) in cats, making FIV a potential small animal model for studying the pathogenesis of human AIDS. Furthermore, due to its phylogenetic distance from primate retroviruses like human immunodeficiency virus (HIV) and its ability to infect non-dividing cells, FIV is also being exploited for the development of vectors for human gene therapy. Therefore, it is of paramount importance that pertinent steps in the life cycle of FIV, such as genomic RNA (gRNA) packaging, are delineated at the molecular level. The process of retroviral gRNA packaging relies on …

Investigating Evidence In Support Of Validity And Reliability For Data Collected With The Meaningful Learning In The Laboratory Instrument (Mlli), Elizabeth Vaughan, A. Montoya-Cowan, Jack Barbera

Chemistry Faculty Publications and Presentations

The Meaningful Learning in the Laboratory Instrument (MLLI) was designed to measure students’ expectations before and after their laboratory courses and experiences. Although the MLLI has been used in various studies and laboratory environments to investigate students’ cognitive and affective laboratory expectations, the authors of the instrument reported a discrepancy between the intended factor structure of the MLLI and the factor structure suggested by the data collected in preliminary studies. Therefore, the aim of this study was to investigate the validity and reliability evidence related to data collected with the MLLI, especially that related to structural validity. Evidence to support …

Potent Bile Acid Microbial Metabolites Modulate Clostridium Perfringens Virulence, Tahrir Jatli Alenezi, Ying Fu, Bilal Alrubaye, Thamer Alanazi, Ayidh Almansour, Hong Wang, Xiaolun Sun

Poultry Science Faculty Publications and Presentations

Clostridium perfringens is a versatile pathogen, inducing diseases in the skin, intestine (such as chicken necrotic enteritis (NE)), and other organs. The classical sign of NE is the foul smell gas in the ballooned small intestine. We hypothesized that deoxycholic acid (DCA) reduced NE by inhibiting C. perfringens virulence signaling pathways. To evaluate the hypothesis, C. perfringens strains CP1 and wild-type (WT) HN13 and its mutants were cultured with different bile acids, including DCA and isoallolithocholic acid (isoalloLCA). Growth, hydrogen sulfide (H₂S) production, and virulence gene expression were measured. Notably, isoalloLCA was more potent in reducing growth, H …

Editorial: Parasitism: The Good, The Bad And The Ugly, Marco A. Juarez-Estrada, Danielle Graham, Xochitl Hernandez-Velasco, Guillermo Tellez-Isaias

Poultry Science Faculty Publications and Presentations

Editorial on the Research Topic

Parasitism: the good, the bad and the ugly

Firearm Contagion: A New Look At History, Rachel Martin, Michael R. Ulrich

Faculty Scholarship

Gun violence is widely considered a serious public health problem in the United States, but less understood is what this means, if anything, for evolving Second Amendment doctrine. In New York Pistol & Rifle Association, Inc. v. Bruen, the Supreme Court held that laws infringing Second Amendment rights can only be sustained if the government can point to sufficient historical analogues. Yet, what qualifies as sufficiently similar, a suitable number of jurisdictions, or the most important historical eras all remain unclear. Under Bruen, lower courts across the country have struck down gun laws at an alarming pace, while …

Need-Oriented Evangelism: Encountering, Embracing, And Experiencing God, Petr Cincala, Allison Sauceda

Faculty Publications

This article provides a fresh perspective on evangelism by reflecting a belief in the Triune God--the source of saving truth and loving community. Mission is part of God’s nature; he is the ultimate Missionary. Mission happens naturally whenever children of God intentionally invite and facilitate God’s presence. Over time, the word “evangelism" has become contaminated by myths, a false understanding of God, and unpleasant religious experiences and practices. Genuine evangelism addresses the most burning issues of people’s lives and should be treated as such. True evangelism involves caring about, praying for, and sharing with people. The practical part of this …

Editorial: An Unexpected Event Related To The Aquatic Mammals Of Latin America, Daniel Gonzalez-Socoloske

Faculty Publications

No abstract provided.

Cyber Fraud Profiling With Routine Activity Theory Using Data Mining Techniques, Sunardi Sunardi, Abdul Fadlil, Nur Makkie Perdana Kusuma

Malaysian Journal of Computing (MJoC)

Cyber profiling, as one of the supporting parts of digital forensics, is not only used to record and investigate cybercriminal behaviour. It can also be used to profile victim demographics based on victim characteristics. This study aims to create a cyber-fraud pattern based on a profile created from RAT. This research is expected to be input for internet users in Indonesia, especially IM users such as Instagram, Facebook, WhatsApp, and Telegram. The data collection method in this study uses data mining technology on structured and unstructured data. Structured data was obtained by conducting data mining on the number of cases …

Modelling Time Series Tropospheric Ozone Data And The Precursors To Study Effect And Relationship, Emilya Shahira Azli, Nurazrin Syafiqah Mat Asri, Siti Nurliyani Ahmad Tajuddin, Norshahida Shaadan, Siti Afiqah Muhamad Jamil, Shamsiah Sapri

Malaysian Journal of Computing (MJoC)

Understanding the role of pollutant precursors is very important to provide meaningful insights for planning and designing localized air pollution control strategies.  This study is conducted with the aim of describing the relationship and assessing the effect of several precursors (OX, NO, NO2, SO2, CO) towards O3 using Multiple Linear Regression (MLR) at an industrial (Petaling Jaya) and an urban (Shah Alam) location in Selangor, Peninsular Malaysia. Statistical modelling and analysis were conducted based on secondary data of time-series observations of multi-variables from the period of the year 2015-2017 obtained from the Department of Environment, Malaysia. Two models were developed, …