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Transformation Efficiency And Gfp Expression In E. Coli, Jessica H. Hobbs

Honors Theses

Genetic transformation of bacteria is a foundational technique in molecular biology and biotechnology, enabling the introduction and expression of foreign genes for research, industrial, and educational applications. This thesis investigates the transformation efficiency and green fluorescent protein (GFP) expression in Escherichia coli using the pGLO plasmid system, with the primary objective of supporting the development of a graduate-level laboratory course focused on bioprocess engineering and molecular biotechnology. The pGLO plasmid contains a GFP reporter gene regulated by an arabinose-inducible promoter and an ampicillin resistance gene that allows for selective growth of transformed cells. Competent E. coli HB101 K-12 cells were …

Implementation Of Pathways For Standing Order Sets Applied Using Nurse Driven Protocols In An Outpatient Oncology Infusion Clinic, Katie Magni, Kellie Olsen, Ijeoma Eche-Ugwu, Jeanine Rundquist

Doctor of Nursing Practice Scholarly Projects

No abstract provided.

Determinants Of Hypertension Awareness And Control In Kenya: A Secondary Analysis Of National Survey Data, Alexander K. Davis

Honors Theses

In low- and middle-income countries (LMICs), cardiovascular diseases (CVDs) are prevalent and pose a major concern as the current healthcare systems are oriented primarily towards communicable diseases. The largest contributor to CVDs, hypertension, is a significant issue in Kenya, a model LMIC. Based on previous studies in this lab, high rates of hypertension are present in the region of Kasigau, Kenya. Kasigau is a small region within Taita-Taveta County, Kenya. Traditional risk factors are not necessarily predictive of hypertension in all regions of Kenya. This study aims to address the lack of tested risk factors for hypertension in Kenya and …

Public Policy And Food Security: A New England–Maritime Provinces Comparative Analysis Of Nutrition Assistance Programs, Kellie A, Therriault

Muskie School Capstones and Dissertations

This study examines how differences in policy design and program administration influence food assistance access and potential food insecurity outcomes across Northern New England (Maine, New Hampshire, and Vermont) and the Canadian Maritime provinces (New Brunswick, Nova Scotia, and Prince Edward Island). Using a mixed methods comparative design, the analysis integrates 2023 administrative data with qualitative insights from semi-structured interviews with program administrators. Key indicators include program participation, household composition, benefit structure, and eligibility thresholds. Results indicate that SNAP participation varies widely across U.S. states and is associated with differences in average income levels, while Canadian provinces demonstrate more consistent …

Developing A Decision-Making Tool For Breast-Feeding Mothers Whose Babies Have A Cow's Milk Protein Allergy, Rachel M. Griffith

Human Nutrition and Hospitality Management Undergraduate Honors Theses

Cow’s milk protein allergy (CMPA) is one of the most common food allergies in infancy, affecting an estimated 2–3% of infants, including those who are breastfed. Many symptoms of CMPA overlap with gastrointestinal disorders and lactose intolerance, leading to frequent misdiagnosis and unnecessary maternal dietary restriction. Breastfeeding mothers who eliminate dairy to manage suspected CMPA often face nutritional deficiencies, emotional strain, and financial burden, while infants risk poor growth if symptoms are not properly addressed. Breastfeeding is viewed as the optimal source of nutrition for infants providing many cognitive benefits and positive maternal effects like decreased stress and risks of …

Faculty And Academic Advisor Perspectives On Embedded Certifications Supporting Neurodivergent Learners, Katherine B. Watkins

Human Nutrition and Hospitality Management Undergraduate Honors Theses

This study explores the experiences, strengths, and challenges of neurodivergent individuals within educational and social environments, with a focus on promoting inclusivity and understanding. Neurodivergence, encompassing conditions such as autism, ADHD, dyslexia, and other cognitive variations, is increasingly recognized not as a deficit but as a natural form of human diversity. The research examines how traditional systems often fail to accommodate diverse cognitive styles, leading to barriers in learning, communication, and self-expression. Using a mixed-methods approach, the study combines quantitative survey data with qualitative interviews to capture both broad trends and personal narratives. Participants reported that rigid structures, sensory overload, …

Enhancing Food Security In Stann Creek. Belize Using Meat Rabbits, Lindsey Garretson

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

In recent years, food insecurity and the prevalence of hunger have rapidly grown throughout the world, but especially within Latin America and the Caribbean. Along with growing rates of food insecurity and hunger there are increases in medical conditions including negative impacts the functioning of every major organ system, increased rates of mental illness, both overweight and underweight children, wasting in both children and adults, and anemia. Availability and access to healthy, nutritional food options can help lower the risk of these conditions. With these considerations, the goal of this project was to create an all-encompassing manual and construction guide …

The Effects Of Cannabidiol (Cbd) On The Social, Cognitive, And Physical Facets Of Anxiety Sensitivity, Ezri Rathbun

Psychological Science Undergraduate Honors Theses

The current study investigated how repeated administration of 300mg of cannabidiol (CBD), when compared to a placebo, affects the social, cognitive, and physical facets of anxiety sensitivity (AS), a characteristic that reflects fear of the consequences of anxious arousal. Prior work suggests CBD may have anxiolytic effects that may be specific to the social aspects of anxiety. Thus, it was hypothesized that, that, relative to placebo, CBD would reduce self-reported AS-social concerns, but not AS-cognitive or AS-physical concerns, compared to baseline. Participants (N= 79; 52 women; M_age = 22.06) with elevated perceived stress were randomly assigned to 14 …

Evaluating The Impact Of Intercropping Vegetables And Cut Flowers On Yield And Conservation Biological Control, Alliance Igiraneza

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Specialty crops such as vegetables are some of the most susceptible to yield losses and increased management difficulty over time due to pests. One of the potential solutions to this problem, which aims at ensuring the sustainability of the sector, is using conservation biological control practices such as intercropping to increase diversity, while maintaining the economic feasibility and profitability of the system. This study was conducted to evaluate the impact of this management practice using Bell pepper (Capsicum annuum var. King Arthur) as the main crop, intercropped with flowers including zinnia (Zinnia elegans var. Benary’s Giant), sunflower ( …

Memoir: The Brain, Neuroethics, And Bioethics, Leslie C. Griffin

North Carolina Law Review

No abstract provided.

Soil Property Responses To Push-Pull Cropping In East Africa, Grace Mercy Amboka, Mattias Jonsson, Celina Apel, David Meinhof, Adomas Liepa, Frank Chidawanyika, Andargachew Detebo, Felipe Librán-Embid, Emily A. Martin, Ingolf Steffan-Dewenter, Marcell K. Peters, Jie Zhang, Michael Thiel, Michael Otim, James Mugisha, Ghebremedhin Belay Bahta, Fredah Maina, Alice Murage, Benjamin Feit, A. Sigrun Dahlin

All Peer-Reviewed Publications

Push-pull technology is increasingly promoted in sub-Saharan Africa, particularly for pest management and enhancing crop productivity. However, its influence on soil properties remains understudied, despite its potential implications for soil health and sustainable soil fertility management. This study examines soil properties in push-pull and conventional non-push-pull cropping systems. Soil samples were collected from push-pull and conventional plots in Ethiopia, Kenya, Rwanda, and Uganda. We examined the associations between soil physicochemical properties and cropping systems, along with key components of push-pull, namely Desmodium coverage and plot age, and manure and mineral fertiliser application. Overall, there were a few differences in soil …

Identification, Pathogenicity, And Fungicide Sensitivity Of Itersonilia Perplexans Associated With Petal Spot Of Gerbera Cut Flowers In Colombia, Matheus Beger

All Theses

Petal diseases significantly affect gerbera cut flower production, as minor visual damage renders flowers unmarketable. Among those pathogens causing petal spots, Itersonilia perplexans affects some Asteraceae plants. However, this pathogen's impact on Colombian gerbera remains poorly studied. This study was designed to fill four gaps: molecular identification of Colombian isolates, confirmation of pathogenicity, standardized culture media, and fungicide sensitivity data. Four objectives were pursued: (i) isolate and identify the causal agent through sequencing the Internal Transcribed Spacer, Small Subunit and Large Subunit from the rDNA; (ii) evaluate pathogenicity on detached gerbera petals; (iii) identify optimal culture media for I. perplexans …

An Economic Analysis Of Sustainability Efforts In The Personal Care And Cosmetics Industry, Anna Briscoe

Harriet L Wilkes Honors College Theses 2020 - Present

Sustainability has become a topic of interest in the personal care and cosmetic industry due to current climate crises and increased consumer awareness. While many definitions of sustainability exist, this paper focuses on sustainability from economic, environmental, and societal perspectives. In recent years, interest in understanding how sustainability efforts impact consumer’s willingness to pay and a firm’s long term success has grown. This research paper examines current sustainability efforts from top firms in the industry, changes in consumer behavior, and the potential economic long term benefits and drawbacks of these efforts. iv

Not Just A Drink: The Cultural Evolution Of A Beverage Turned Lifestyle And Its Adaption To Spain, Ava Axtell

Marketing Undergraduate Honors Theses

This research explores the rise of dirty soda in the United States and how a simple customizable beverage evolved into a cultural ritual shaped by social media, treat culture, and the lifestyle norms of the LDS community. It analyzes the cultural meaning behind Swig’s popularity, including how influencers and online aesthetics transformed the drink into a status symbol and daily indulgence. This study also compares U.S. and Spanish cultural values, including individualism, indulgence, and social rituals, to determine how Swig must adapt its product, branding, and store model for successful expansion into Spain. Findings reveal that while dirty soda thrives …

Disruption Of Energy Homeostasis By Chemical Mixtures Containing Persistent Pollutants In Hepg2 And C2c12 Cells, Morgan Jacobellis

All Theses

The Environmental Influences on Child Health Outcomes Fetal Growth Studies (ECHO-FGS) birth cohort data was used to identify chemical mixtures associated with adverse outcomes. This biomonitoring study associated specific chemical mixtures in maternal serum with low birth weight or childhood obesity (Pearce et al., 2021). Mixture Profiles 1, 3, and 4, comprised of different observed concentrations of DDE, PCB153, BDE47, and PFOS were prioritized. Mixtures were tested to determine whether they perturbed mitochondrial metabolism and energy sensing pathways. The purpose of this study was to elucidate a potential mechanism linking maternal exposure to mixtures containing persistent organic pollutants (POPs) to …

Field Pea (Pisum Sativum L.) Response To Planting Date In South Carolina Organic Cropping Systems, Tristan Lawrence

All Theses

Field peas (Pisum sativum L.) are not widely cultivated in South Carolina, resulting in limited agronomic data to support farmers interested in this crop. However, field peas hold significant promise as a cool season crop for both organic and conventional growers in the region. Most commercially available field pea cultivars are evaluated and bred for the Pacific Northwest, where environmental conditions differ substantially from those in the southeastern United States. Consequently, there is a lack of region-specific recommendations for successful cultivation in South Carolina. To address this gap, a two-year planting date study was conducted during the …

A Smoking-Related Plasma Protein Score And Smoking-Related Cancer Risk And Mortality In The Atherosclerosis Risk In Communities Study, Meng Ru, Christopher Douville, Aghiles Guenoun, Hana Zahed, Christie M Ballantyne, Kenneth R Butler, Josef Coresh, David J Couper, Panagis Galiatsatos, Marc J Gunter, Ron C Hoogeveen, Mattias Johansson, Corinne E Joshu, P Martijn Kolijn, Christina M Lill, Jiayun Lu, Michael T Marrone, Giovanna Masala, David C Muller, Anna E Prizment, Raul Zamora-Ros, Nilanjan Chatterjee, Adrienne Tin, Elizabeth A Platz

Faculty, Staff and Students Publications

Background: Self-reported smoking may not fully capture individualized risk of smoking-related cancer, but circulating proteins may reflect biological consequences of smoking. Thus, we developed a score based on smoking-related proteins and evaluated its association with smoking-related cancer.

Methods: This prospective cohort study included 10 563 participants aged 47-70 years in the Atherosclerosis Risk in Communities study. Plasma proteins were measured using the SomaScan Platform (SomaLogic Operating Co, Inc). The score was constructed from proteins associated with current smoking, pack-years, or recent quitting identified by linear regression and elastic net regression. Cox regression was used to estimate adjusted hazard ratios and …

The Prevalence Of Amenorrhea In Relation To Red-S Syndrome Among Various Sports In Collegiate Female Athletes, Ruby S. Holland, Marilou D. Shreve

The Eleanor Mann School of Nursing Undergraduate Honors Theses

Background: RED-S is a common risk factor for amenorrhea in female athletes. Athletes that compete in high-intensity sports or without adequate caloric intake, are at risk for low energy expenditure which may disrupt hormonal balance. As development of RED-S is a well-established risk factor for amenorrhea, early detection and correction is crucial to prevent long term health consequences.

Purpose: The purpose of this research is to explore if there is a correlation between RED-S score and amenorrhea in female athletes.

Methods: An online survey was distributed to approximately 220 female athletes at the University of Arkansas using Qualtrics. The 69-question …

Plasma Proteins Associated With Psychosocial Factors And Heart Disease: The Jackson Heart Study, Sara N O'Brien, Madeline G Gillman, Michael D Green, Daniel E Cruz, James S Floyd, Susan Hankinson, Daniel H Katz, Xiaojuan Liu, Michelle C Odden, Bruce M Psaty, Alexander P Reiner, Jerome I Rotter, Stephen S Rich, Robert E Gerszten, Amil Shah, Mario Sims, Usman A Tahir, Lesley F Tinker, Alexis C Wood, Bing Yu, Anthony S Zannas, Laura M Raffield, Láshauntá M Glover

Faculty, Staff and Students Publications

Background: Knowledge of proteomic mechanisms explaining the link between psychosocial stress and cardiovascular disease is limited. This study aimed to (1) identify plasma proteins associated with psychosocial factors and (2) assess associational pathways between psychosocial factors, identified proteins, and incident cardiovascular disease events in a discovery cohort, JHS (Jackson Heart Study), and 2 replication cohorts, the CHS (Cardiovascular Health Study), and the MESA (Multi-Ethnic Study of Atherosclerosis).

Methods: JHS participants from exam 1 (2000-2004) with SomaScan 1.3k platform proteomics data were included (n=2143, mean age=55.3). Depressive symptoms and perceived stress scores were measured via the 20-item Center for Epidemiological Studies …

Effects Of Mineral Supplementation On Ovine Performance, Forage Selectivity, And Rangeland Utilization In Southern Utah, Ryan A. Spurling

All Graduate Theses and Dissertations, Fall 2023 to Present

Sheep producers in the Intermountain West region of the US rely on productive rangeland ecosystems that vary widely in forage composition and nutrient availability. As such, supplementation is often necessary to offset dietary deficiencies. Soils and forages grown on rangelands are known to be highly variable in mineral content. Even so, a third to half of the producers in the Intermountain West choose not to provide a complete mineral supplement. Rangeland environments are often characterized by rugged geographical features that make optimal supplement placement difficult. New advances in technology have allowed us to measure individual animal intake on rangelands via …

Paucilactobacillus Wasatchensis Gas Production In Cheddar Cheese Through Symbiotic Amino Acid Metabolism With Lactococcus Lactis Starter Cultures, Niharika Mishra

All Graduate Theses and Dissertations, Fall 2023 to Present

Cheese is popular for its rich sensory notes and desirable physical attributes; however, the late blowing gas defect in cheddar cheese disrupts its appearance and can undermine its commercial value, for example if the late gas defect is severe, the block can crumble upon cutting, increasing cutting losses from 10% (non-defective) up to 50% (Donnely et al., 2014).

Paucilactobacillus wasatchensis cannot survive pasteurization (McMahon, D. J., et al., 2020); therefore, it enters the cheese as a contaminant during the cheesemaking process. Cheesemaking occurs in three stages: (1) curdling of milk by acidification through starter cultures, (2) separation of curd from …

Seasonal Body Composition And Neuromuscular Changes In High School Wrestlers: Medicine Balls For Fatigue Testing, Courtney Mccall Condie Carroll

All Graduate Reports and Creative Projects, Fall 2023 to Present

This study examines the effects a single wrestling season has on high school athletes’ body composition, muscle quality, membrane capacitance, and neuromuscular performance, and whether instrumented medicine balls are sensitive enough to serve as a field test for chronic fatigue. Twenty-five high school wrestlers (males=18, females=7) were recruited and assessed pre-season (Pre), mid-season (Mid), and end-of-season (Post). Outcome measures included body mass, fat-free mass, fat mass (via bioelectrical impedance spectroscopy), echo intensity (EI, via B-mode ultrasound) of the vastus lateralis (VL) and biceps brachii (BB), and countermovement rotational and backward-overhead throws with medicine balls instrumented with accelerometers.  Repeated measures ANOVA …

Grazed Pasture Effects On Greenhouse Gas Emissions In The Ozark Highlands, Tyler Buchanan

Graduate Theses and Dissertations

In the United States (US), grazinglands serve as the main foundation for the livestock industry, and, in Arkansas specifically, pastures are essential for rotational grazing and dairy operations. Climate change is increasingly becoming a concern in the agriculture industry, largely caused by anthropogenic activities increasing greenhouse gas (GHG) concentrations in the atmosphere, at least partly due to the nutrient recycling that occurs from animal manure additions in grazinglands. Therefore, quantifying GHG emissions from pastures is essential to better understanding the impacts of grazing on the environment. The objective of this study was to quantify and evaluate the potential effects of …

Dissecting The Complex Genomic Landscape Underlying Oxidative Stress Resistance Using Natural Variation In Yeast, Sonali Lakshika Anne Lenaduwe

Graduate Theses and Dissertations

Stress is experienced across the tree of life. Survival in fluctuating environments depends on a robust genetic architecture. This dissertation examines the genetic and metabolic determinants underlying one such adaptive response: cross-protection. Leveraging natural variation in the stress responses of budding yeast, Saccharomyces cerevisiae, I examine the genetic variation contributing to ethanol-induced cross-protection against oxidative stress. Acquired stress resistance, in which mild pre-exposure improves survival to subsequent stresses, is prevalent across diverse organisms and can involve both same-stress and cross-protection. Using high-throughput bulk segregant analysis (BSA-seq) and advanced intercross mapping populations derived from natural yeast isolates, I identify quantitative trait …

Impacts Of Climate-Smart Agriculture On Greenhouse Gas Emissions In Southeast Arkansas Cotton, Cassandra Seuferling

Graduate Theses and Dissertations

Increasing concerns of climate change have led to the evaluation of greenhouse gas (GHG) emissions [i.e., carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O)] from agroecosystems, particularly cotton (Gossypium hirsutum) production. Technology improvement and conservation agriculture methods [i.e., reduced-tillage (RT) instead of conventional tillage (CT) and the use of cover crops (CC)] require further evaluation regarding GHG emissions from Arkansas cotton production. The first objective of the study was to evaluate four gas-flux-determination methods [i.e., linear or exponential regression models, with negative fluxes (WNF) included in the dataset or replacing negative fluxes (RNF)] on CO2, CH4, and N2O fluxes …

Herbivory Of Phragmites Australis Along A Salinity Gradient In Connecticut Wetlands, Sydney H. Kolz

Honors Scholar Theses

Invasive Phragmites australis (common reed) is widely distributed in New England wetlands, where it often outcompetes native plants and decreases biodiversity throughout the food web. Controlling its invasion is a priority, especially in salt marsh habitat, but current management techniques are inefficient. Leveraging natural processes may improve control strategies, but in order to do so, we must first better understand how abiotic and biotic stressors interact and affect Phragmites. This study focuses on herbivory and salinity, two factors limiting Phragmites’ fitness. I measured soil salinity and quantified the damage of three herbivore guilds (i.e. gall-formers and stem-borers, leaf-chewers, and …

Addressing Postsecondary Lack Of Preparedness Through Exposure And Empowerment, Deztiny A. Ochoa

Capstone Projects and Master's Theses

Too many high school students are not college or career ready when they graduate. At Learning For Life Charter School, the school counselor and the intern worked together to implement a project to address the issue. The community served was 7th graders to 12th graders. A lot of these students were on the verge of dropping out or completely dropped out of traditional school. The capstone project consisted of taking 10 students on a field trip to a community college nearby. The purpose of this trip was to give students the opportunity to see their options after high school and …

Reducing Childhood Dental Decay: Fluoride Education Campaign With The Monterey County Health Department, Shelby L. Minter

Capstone Projects and Master's Theses

Dental decay is a significant problem for youth across Salinas, contributing to preventable oral health disparities. The Monterey County Health Department Oral Health Program provides oral health education and prevention services across Monterey County. This project focused on improving parental knowledge and confidence about fluoride usage in areas where pediatric dental caries rates are high. Its purpose was to support the prevention of dental decay through the promotion of fluoride benefits. Two educational campaigns were conducted at elementary schools in Salinas; Monte Bella Elementary School and Alisal Community School. The expected outcomes were increased knowledge, confidence, and positive attitudes towards …

Improving Fafsa Completion For High School Graduates, Haylee B. Padilla

Capstone Projects and Master's Theses

Too many students at Learning For Life Charter School graduate without a clear understanding of social mobility and are unprepared for the academic and practical demands of college. This project addressed the need for increased FAFSA completion and college and career readiness among 11th and 12th grade high school students graduating in the 2026 school year. The purpose of this project was to provide support to help students get federal student aid by walking them through filling out the FAFSA form and giving clear steps so they could apply without stress. Key activities included workshops, one-on-one support guiding students through …

Adapting Preventive Health Education For Teens, Marcela G. Daniel

Capstone Projects and Master's Theses

Too many low-income individuals are developing type 2 diabetes. Limited health education and socioeconomic barriers increase the risk of type 2 diabetes among Monterey County residents. This capstone project was conducted at Community Health and Wellness, a department of Montage Health that provides prevention-focused programs, including Healthy Together. The project served middle and high school students (ages 12–18) from communities across Monterey County. The purpose of the capstone project was to gather teen input on how to improve a teen-focused prevention program and increase engagement among participants. The project was implemented through a survey distributed in local schools, with the …