Digital Commons Network^™

Chagas Disease In Immunocompromised Patients, Eva H Clark, Louisa A Messenger, Jeffrey D Whitman, Caryn Bern

Faculty, Staff and Students Publications

As Chagas disease remains prevalent in the Americas, it is important that healthcare professionals and researchers are aware of the screening, diagnosis, monitoring, and treatment recommendations for the populations of patients they care for and study. Management of Trypanosoma cruzi infection in immunocompromised hosts is challenging, particularly because, regardless of antitrypanosomal treatment status, immunocompromised patients with Chagas disease are at risk for T. cruzi reactivation, which can be lethal. Evidence-based practices to prevent and manage T. cruzi reactivation vary depending on the type of immunocompromise. Here, we review available data describing Chagas disease epidemiology, testing, and management practices for various …

Development Of Biochemical And Cellular Probes To Study Ripk1 Target Engagement, Xin Yu, Hanfeng Lin, Feng Li, Jin Wang, Dong Lu

Faculty, Staff and Students Publications

RIPK1 inhibitors have emerged as promising candidates for treating diverse diseases, including inflammatory diseases, autoimmune disorders, Alzheimer’s disease, and cancer. However, the previously reported binding assays have limited sensitivity and stability, impeding high-throughput screening and robust characterization of the RIPK1 inhibitors. To address this challenge, we introduced two probes, T2-BDP-FL and T3-BDP-FL, derived from distinct RIPK1 inhibitors with different binding modes to establish time-resolved fluorescence resonance energy transfer (TR-FRET) displacement assays. Employing our TR-FRET displacement assays, we quantified the biochemical binding affinities of a series of RIPK1 inhibitors with diverse structural and binding modes for human RIPK1. Consistent results were …

A Cost Variation Analysis Of Antibiotics Available For The Management Of Community Acquired Pneumonia, Outpatient Setting In Egypt, Yara Khalaf, Sahar Al Shabasy, Samar Farid

Bulletin of Faculty of Pharmacy Cairo University

Background

Around 71.8% of all expenditures in Egypt are out-of-pocket expenses for health care. Almost all medications, including antibiotics, come in a variety of brand names with varying prices.

Objective

To assess the variation in prices of different brands of antibiotics used for the management of adult community-acquired pneumonia in the outpatient settings.

Patients and procedures

List of antibiotics selected using guidelines for the treatment of community-acquired pneumonia in adults at the outpatient setting and those antibiotics specified in the national list of essential medicines (NLEM) 2022, Egypt, was prepared. Percentage cost variation and cost ratio of different brands of …

Around The Center, 1993 April, Advocate Health - Midwest

Historical Documents - Combined

Illinois Masonic Medical Center, Chicago, IL: Issue of "Around the Center", an employee newsletter. This issue has staffing and departmental updates, patient letters, and features on the new Wilton Building and on the employee fitness program.

Around The Center, 1992 March, Advocate Health - Midwest

Historical Documents - Combined

Illinois Masonic Medical Center, Chicago, IL: Issue of "Around the Center", an employee newsletter. This issue has staffing and departmental updates, patient letters, and a feature on the medical genetics department.

Entry-Level Occupational Therapy Student Volunteer Activities During Covid-19 And Beliefs About Practitioner Roles In Crisis Management, Olivia Biller, Frances Calingo, Tina M. Deangelis, Maclain Capron Ba

Student Journal of Occupational Therapy

Background: Due to the global novel coronavirus disease 2019 (COVID-19) pandemic, many students in entry-level occupational therapy programs faced changes to their daily roles, habits, and routines. To understand beliefs about OT practitioner roles in a public health crisis, a cross-sectional study was designed to understand occupational therapy students (OTS) and occupational therapy assistant students (OTAS) volunteer activities during the COVID-19 pandemic.

Methods: A survey was developed and distributed in April 2020, and closed after five weeks. Quantitative data was analyzed in Statistical Package for Social Sciences (Version 27) and open response data was categorized in Microsoft Excel.

Results: Respondents …

Assessment For Sustainable Transportation Systems In Egypt, Hagar Mohamed Fares

Theses and Dissertations

The assessment of a sustainable transportation system poses a significant challenge for local governments, as it dramatically influences the promotion of environmentally friendly modes of transportation with the objective of safeguarding the public's welfare and health in their daily activities. The integration of such assessment should be implemented from the initial stages of system planning to its operational and maintenance phases, particularly in light of the increasing number of urban development activities nationwide. In order to facilitate decision-making processes, it is imperative to establish an efficient rating system that incorporates cultural and social constraints and comprehensively considers the three dimensions …

Comparison Of Radiologic Findings Between Sars-Cov-2 And Other Respiratory Tract Viruses In Critically Ill Children During The Covid-19 Pandemic, Oktay Perk, Tanil Kendi̇rli̇, Emel Uyar, Bi̇rsel Şen Akova, Hati̇ce Albayrak, Hasan Ağin, Ebru Ati̇ke Ongun, Eşe Eda Turanli, Sare Güntülü Şik, Şahi̇n Si̇ncar, Gürkan Bozan, Demet Demi̇rkol, Nazan Ülgen Tekerek, Mey Tali̇p, Arzu Oto, Feyza İnceköy Gi̇rgi̇n, Ferhat Sari, Nuretti̇n Onur Kutlu, Altan Güneş, Ömer Suat Fi̇töz

Turkish Journal of Medical Sciences

Background/aim: This study was planned because the radiological distinction of COVID-19 and respiratory viral panel (RVP)-positive cases is necessary to prioritize intensive care needs and ensure non-COVID-19 cases are not overlooked. With that purpose, the objective of this study was to compare radiologic findings between SARS-CoV-2 and other respiratory airway viruses in critically ill children with suspected COVID-19 disease.Materials and methods: This study was conducted as a multicenter, retrospective, observational, and cohort study in 24 pediatric intensive care units between March 1 and May 31, 2020. SARS-CoV-2- or RVP polymerase chain reaction (PCR)-positive patients’ chest X-ray and thoracic computed tomography …

How Working Women In Healthcare Perceive Public Transportation And Safety, Assaaformbi Bama

Walden Dissertations and Doctoral Studies

Women use public transportation more frequently than do men. However, most of these transportation systems were not constructed with women in mind. Public transportation systems are accountable for securely transporting people to their destinations, as well as upkeeping protective policies to advocate for fearful passengers. The purpose of this qualitative study was to examine women’s perspectives on public transportation.The research question guiding this study asked women to discuss their safety experiences and needs related to existing public transportation policy. Cooley’s theory of transportation and Friedman’s public safety theory formed the conceptual framework for this study. Purposeful sampling was used to …

How Working Women In Healthcare Perceive Public Transportation And Safety, Assaaformbi Bama

Walden Dissertations and Doctoral Studies

Women use public transportation more frequently than do men. However, most of these transportation systems were not constructed with women in mind. Public transportation systems are accountable for securely transporting people to their destinations, as well as upkeeping protective policies to advocate for fearful passengers. The purpose of this qualitative study was to examine women’s perspectives on public transportation.The research question guiding this study asked women to discuss their safety experiences and needs related to existing public transportation policy. Cooley’s theory of transportation and Friedman’s public safety theory formed the conceptual framework for this study. Purposeful sampling was used to …

Short- And Long-Term Neurological Outcomes Of Congenital Cytomegalovirus Infection, Fati̇h Mehmet Aki̇f Özdemi̇r, Yasemi̇n Taşci Yildiz, Rüveyda Gümüşer Ci̇nni̇, Ayşegül Zenci̇roğlu, Deni̇z Yüksel

Turkish Journal of Medical Sciences

Background/aim: Cytomegalovirus (CMV) is the most common congenital viral infection. Although most children with congenital CMV (approximately 85%–90%) are asymptomatic at birth, findings such as sensorineural hearing loss, microcephaly, and neurodevelopmental retardation can be observed during the follow-up. Among the brain magnetic resonance imaging (MRI) findings of CMV are white matter abnormalities, polymicrogyria, and periventricular calcification. Since a definitive diagnosis of congenital CMV cannot be made after the neonatal period, the identification of the associated phenotype is diagnostically important, but data are limited in patients who have been retrospectively diagnosed with congenital CMV infection. The aim of this study was …

Mycoplasmoides Genitalium Nucleic Acid Semi-Quantitation And Molecular Macrolide Resistance Detection Via Automated Assays: Gender And Specimen Source Considerations, Erik Munson, Josephine Moore, Trinity Krueger, Amanda Zapp, Stephen C Lavey, Kimber L Munson, Irene A Stafford, Brian Mustanski

Faculty, Staff and Student Publications

A laboratory-developed test (LDT) using analyte-specific reagents has been optimized on a commercial platform to detect macrolide resistance-associated mutations (MRM) in 23S rRNA from Mycoplasmoides genitalium from primary clinical specimens. In this study, MRM-LDT was applied to a multi-specimen source study set. One thousand four hundred ninety-five primary specimens testing positive for M. genitalium by commercial transcription-mediated amplification (TMA) were initially titered by the TMA assay using serial 10-fold dilutions to semi-quantitate target nucleic acid burden. Primary specimens were then processed for MRM detection using the MRM-LDT. Findings were stratified by gender and specimen source. The mean log10 target nucleic …

Machine Learning In Time-Lapse Imaging To Differentiate Embryos From Young Vs Old Mice†, Liubin Yang, Carolina Leynes, Ashley Pawelka, Isabel Lorenzo, Andrew Chou, Brendan Lee, Jason D Heaney

Faculty, Staff and Students Publications

Time-lapse microscopy for embryos is a non-invasive technology used to characterize early embryo development. This study employs time-lapse microscopy and machine learning to elucidate changes in embryonic growth kinetics with maternal aging. We analyzed morphokinetic parameters of embryos from young and aged C57BL6/NJ mice via continuous imaging. Our findings show that aged embryos accelerated through cleavage stages (from 5-cells) to morula compared to younger counterparts, with no significant differences observed in later stages of blastulation. Unsupervised machine learning identified two distinct clusters comprising of embryos from aged or young donors. Moreover, in supervised learning, the extreme gradient boosting algorithm successfully …

Ensuring Transparency In Using Chatgpt For Public Sentiment Analysis, Chun-Hua Tsai, Gargi Nandy, Deanna House, John M. Carroll

Information Systems and Quantitative Analysis Faculty Publications

The advancement of generative AI, involving the utilization of large language models (LLMs) like ChatGPT to assess public opinion and sentiment, has become increasingly prevalent. However, this upsurge in usage raises significant questions about the transparency and interpretability of the predictions made by these LLM Models. Hence, this paper explores the imperative of ensuring transparency in the application of ChatGPT for public sentiment analysis. To tackle these challenges, we propose using a lexicon-based model as a surrogate to approximate both global and local predictions. Through case studies, we demonstrate how transparency mechanisms, bolstered by the lexicon-based model, can be seamlessly …

Design And Fabrication Of Passive Microfluidic Devices For Enhancing Membrane-Based Biosensors, Tanner N. Wells

Theses and Dissertations

Microfluidic devices and systems have demonstrated wide applicability in sample processing and analysis. The ability to integrate sample processing and analysis into a single platform is a key benefit of microfluidic technologies. This dissertation describes the design and fabrication two types of microfluidic devices developed to enhance membrane-based biosensors. First is a device for collecting particles into a highly confined region, locally enhancing their concentration. Second is a device for size-based particle separation capable of separating differently sized particles into separate streams. Both types of devices are fabricated with a thin top layer, providing a platform for synthetic membrane-based technologies. …

Using Evidenced-Based Practice To Increase Knowledge And Awareness Of Sexually Transmitted Infections, Amanda M. Brickey

Dissertations

The purpose of this quality improvement (QI) project is to increase the knowledge and intent to change practice through education on the importance in assessing sexual health history in patients at a federally qualified health center (FQHC) in Western Washington State by providing continuing education to primary care providers.

The Mrnacalc Webserver Accounts For The N1-Methylpseudouridine Hypochromicity To Enable Precise Nucleoside-Modified Mrna Quantification, Sarah E. Krul, Sean J. Hoehn, Carlos E. Crespo-Hernández

Faculty Scholarship

Nucleoside-modified messenger RNA (mRNA) technologies necessarily incorporate N1-methylpseudouridine into the mRNA molecules to prevent the over-stimulation of cytoplasmic RNA sensors. Despite this modification, mRNA concentrations remain mostly determined through the measurement of UV absorbance at 260 nm wavelength (A260). Herein, we report that the N1-methylpseudouridine absorbs approximately 40% less UV light at 260 nm than uridine, and its incorporation into mRNAs leads to the under-estimation of nucleoside-modified mRNA concentrations, with 5%–15% error, in an mRNA-sequence-dependent manner. We therefore examined the RNA quantification methods and developed the mRNACalc webserver. It accounts for the molar absorption coefficient of modified nucleotides at 260 …

Role Of Ethanolamine Utilization And Bacterial Microcompartment Formation In Listeria Monocytogenes Intracellular Infection, Ayan Chatterjee, Karan Gautam Kaval, Danielle A Garsin

Faculty, Staff and Student Publications

Ethanolamine (EA) affects the colonization and pathogenicity of certain human bacterial pathogens in the gastrointestinal tract. However, EA can also affect the intracellular survival and replication of host cell invasive bacteria such as Listeria monocytogenes (LMO) and Salmonella enterica serovar Typhimurium (S. Typhimurium). The EA utilization (eut) genes can be categorized as regulatory, enzymatic, or structural, and previous work in LMO showed that loss of genes encoding functions for the enzymatic breakdown of EA inhibited LMO intracellular replication. In this work, we sought to further characterize the role of EA utilization during LMO infection of host …

Noda News December 2011

New Orleans Dental Association News

New Orleans Dental Association (NODA) News includes regular columns with regional news impacting the New Orleans membership including: columns from the President and Executive Director, feature articles relating to the field of dentistry, association events and upcoming activities, component news, continuing education opportunities, and classifieds.

Consensus Recommendations For The Use Of Novel Antiretrovirals In Persons With Hiv Who Are Heavily Treatment-Experienced And/Or Have Multidrug-Resistant Hiv-1: Endorsed By The American Academy Of Hiv Medicine, American College Of Clinical Pharmacy: An Executive Summary, David B. Cluck, Daniel B. Chastain, Milena Murray, Spencer H. Durham, Elias B. Chahine, Caroline Derrick, Julie B. Dumond, E. Kelly Hester, Sarah B. Jeter, Melissa D. Johnson, Christin Kilcrease, Wesley D. Kufel, Jeffrey Kwong, Amber F. Ladak, Nimish Patel, Sarah E. Pérez, Jonell B. Poe, Charlotte Bolch, Ian Thomas, Elizabeth Asiago-Reddy, William R. Short

Pharmacy Faculty Scholarship

Treatment options are currently limited for persons with HIV-1 (PWH) who are heavily treatment-experienced and/or have multidrug-resistant HIV-1. Three agents have been approved by the U.S. Food and Drug Administration (FDA) since 2018, representing a significant advancement for this population: ibalizumab, fostemsavir, and lenacapavir. However, there is a paucity of recommendations endorsed by national and international guidelines describing the optimal use (e.g., selection and monitoring after initiation) of these novel antiretrovirals in this population. To address this gap, a modified Delphi technique was used to develop these consensus recommendations that establish a framework for initiating and managing ibalizumab, fostemsavir, or …

Rapid, Direct Screening Of Priority Metal Contaminants In Environmental Systems With Reactive 3 D-Printed Cone Spray Ionization-Mass Spectrometry ( 3 D- Pcsi-Ms ), Jonathan Nzubechi Chilaka

Theses and Dissertations

Trace metal species are relatively common in our environment, and although some are essential to sustain life, exposure to elevated levels of toxic metals can detrimentally affect both human and animal health. Correspondingly, monitoring of such contaminants in environmental systems is critical to public safety. While various methods exist for sensitive and selective metal analysis for such applications, there remains a need for a rapid, cost-effective, and, ideally, portable solution for expedited results and more impactful remediation and sustainability initiatives. Here, we investigate the use of 3D-printed cone spray ionization-mass spectrometry (3D-PCSI-MS), a newer, spray-based ambient ionization method, with specialty …

Complement Propagates Visual System Pathology After Traumatic Brain Injury, Davis Marchant Borucki

MUSC Theses and Dissertations

Background: Traumatic brain injury is associated with the development of visual system disorders, including deficits in visual acuity. Visual deficits can present with delay and worsen over time, suggesting that ongoing neuroinflammatory changes contribute to their development and progression. Complement activation is strongly associated with the neuroinflammatory response after TBI and has been shown to contribute to neuronal and synaptic loss. However, the contributions of complement activation after TBI to vision loss remains unclear.

Methods: We employed an open skull controlled cortical impact model of traumatic brain injury in adult male mice to study how TBI affects vision. Animals were …

Concepts In Animal Parasitology, Scott L. Gardner, Sue Ann Gardner

Zea E-Books Collection

Note that this version of the textbook has been superceded by the one updated in 2026, available at the UNL Pressbooks site.

The hard copy version that is for sale at lulu.com is the old version.

"This is a textbook covering concepts in animal parasitology. It is meant to be used by students, teachers, professors, researchers, and members of the public who are interested in learning about animal parasite biology, systematics, taxonomy, zoogeography, and ecology. The primary intended audience is upper-level undergraduate or graduate university students who have knowledge of basic biology and, particularly, basic animal biology. (863 pages, …

Macrophage-Driven Car T Cell Resistance In B Cell Lymphoma, Sae Bom Lee

USF Tampa Graduate Theses and Dissertations

Chimeric antigen receptor (CAR) T-cell therapy has revolutionized the treatment of patients with relapsed/ refractory large B cell lymphoma (LBCL). Despite its promising efficacy, a subset of patients experiences primary resistance or relapse. In Chapter 2, we explore the tumor microenvironment (TME)-induced resistance mechanisms in patients with LBCL. Analysis of the intratumoral immune infiltrates in pre-infusion tumor biopsies revealed that the levels of immunoregulatory macrophages were elevated in patients who responded poorly to axicabtagene ciloleucel (axi-cel). Using preclinical mouse B cell tumor models, we found that the CAR T-cell-produced interferon-gamma (IFN-γ) enhanced the expression of inducible nitric oxide synthase (iNOS, …

Screening Of Tuberculosis Infection Through Acid-Fast Bacilli Tests Among Waste Workers In Gresik, Indonesia, Budi Utomo, Widati Fatmaningrum, Sulistiawati, Shifa Fauziyah, Chan Chow Khuen, Nur Fadhilah, Teguh Hari Sucipto

Folia Medica Indonesiana

Highlights: 1. This study was the first in Gresik, Indonesia, to conduct mass screening of tuberculosis among waste workers, comprising various demographic profiles. 2. Although the tuberculosis screening of the waste workers showed negative results, mass examination using an acid-fast bacilli smear remains crucial to detect latent infections and prevent transmissions. Abstract Tuberculosis is a major source of illness and mortality worldwide. Therefore, tuberculosis screening is important to increase the number of active case-finding in a community. Bacteriological examinations can be used to initiate community-based active case-finding. Waste workers face a high risk of contracting Mycobacterium tuberculosis due to their …

Self-Swabbing Versus Assisted Swabbing For Viral Detection By Qrt-Pcr: The Experience From Sars-Cov-2 Based On A Meta-Analysis Of Six Prospectively Designed Evaluations Conducted In A Uk Setting, Tom Fowler, David Chapman, Matthias E Futschik, Sarah A Tunkel, Edward Blandford, Elena Turek, Olumide Kolade, Sergio Souza Da Cunha, Andrew Dodgson, Paul Klapper, Malur Sudhanva, Lindsey Davies, Sue Hill, Susan Hopkins, Tim Peto

School of Biomedical Sciences

PURPOSE: In April 2020, the UK Government implemented NHS Test and Trace to provide SARS-CoV-2 quantitative reverse transcription polymerase chain reaction (qRT-PCR) testing for the public, with nose-and-throat swabbing for samples performed by trained staff. Self-swabbing (SS) would allow rapid scale-up of testing capacity and access. Six studies were undertaken to determine whether SS was as effective for detecting SARS-CoV-2 as swabbing performed by trained staff.

METHODS: Six prospective studies were conducted between April-October 2020, using six swab/media combinations. Differences between assisted swabbing (AS) and SS were evaluated for concordance, positivity, sensitivity, cycle threshold (Ct) values and void rates. Statistical …

Protein Nanoparticles As Vaccine Platforms For Human And Zoonotic Viruses, Kush Kumar Pandey, Bikash R. Sahoo, Asit K. Pattnaik

Nebraska Center for Virology: Faculty Publications

Vaccines are one of the most effective medical interventions, playing a pivotal role in treating infectious diseases. Although traditional vaccines comprise killed, inactivated, or liveattenuated pathogens that have resulted in protective immune responses, the negative consequences of their administration have been well appreciated. Modern vaccines have evolved to contain purified antigenic subunits, epitopes, or antigen-encoding mRNAs, rendering them relatively safe. However, reduced humoral and cellular responses pose major challenges to these subunit vaccines. Protein nanoparticle (PNP)-based vaccines have garnered substantial interest in recent years for their ability to present a repetitive array of antigens for improving immunogenicity and enhancing protective …

The Emerging Biomarkers In Chronic Obstructive Pulmonary Disease: A Narrative Review, Kaitlin M Phillips, Philip F Lavere, Nicola A Hanania, Muhammad Adrish

Faculty, Staff and Students Publications

The burden of chronic obstructive pulmonary disease (COPD) is increasing, especially for women in low-to-middle income countries. Biomarkers provide ever-increasing diagnostic precision for COPD and show promise for primary, secondary, and tertiary disease prevention. This review describes emerging applications for biomarkers in COPD, especially as they align with the Global Initiative for Chronic Obstructive Lung Disease (GOLD) emphasis on prevention, early diagnosis, and response to therapy. These biomarkers include blood eosinophils; IgE; C-reactive protein; fibrinogen; procalcitonin; interleukins 6, 8, and 33; tumor necrosis factor alpha; and soluble receptor for advanced glycated products (sRAGE). They have been used in various ways …

Community-Acquired Pneumonia: The Importance Of The Early Detection Of Drug-Resistant Organisms, Sai Doppalapudi, Muhammad Adrish

Faculty, Staff and Students Publications

Pneumonia is a disease associated with significant healthcare burden with over 1.5 million hospitalizations annually and is the eighth leading cause of death in the United States. While community-acquired pneumonia (CAP) is generally considered an acute time-limited illness, it is associated with high long-term mortality, with nearly one-third of patients requiring hospitalization dying within one year. An increasing trend of detecting multidrug-resistant (MDR) organisms causing CAP has been observed, especially in the Western world. In this editorial, we discuss about a publication by Jatteppanavar

Establishment Of Bacteroides In The Gut And Its Role In The Cystic Fibrosis Gut-Lung Axis, Rebecca A. Valls

Dartmouth College Ph.D Dissertations

In the quest to understand the complex interplay between the gut microbiota and cystic fibrosis (CF), a disease characterized by chronic upper respiratory complications and an exacerbated proinflammatory response, our lab engaged in a collaborative study with Dartmouth-Hitchcock Medical Center (DHMC) to characterize the gut microbiota of pediatric CF patients. Initial analyses established a connection between gut microbial composition and respiratory health, particularly highlighting a scarcity of Bacteroides species compared to healthy controls. In following investigations, we utilized random forest models to understand how stool microbiota may influence clinical outcomes of these pediatric CF patients, including: age, upper respiratory infections, …