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Research Highlights, Shatakshi Bhardwaj, Archna Nagar

Indian Journal of Research in Homoeopathy

Research Highlights

The Role Of L-Fucose In Dendritic Cell Antigen Presentation And Polarization, Chase J. Burton

USF Tampa Graduate Theses and Dissertations

Since the FDA approval of IL-2 in cancer treatment in the 1980s, immunotherapy has emerged as the standard of care for several tumor types (e.g., melanoma, lung, bladder, colorectal). However, approximately 70 percent of cancer patients, independent of prior treatment or staging, administered immunotherapy will not achieve complete responses and will exhibit disease progression. Several factors have been identified to contribute to treatment failure, including the accumulation of intratumoral immunosuppressive cell populations, insufficient tumor-infiltrating anti-tumor immune populations, or the occurrence of immunoediting on tumor cells. Current strategies to circumvent immunosuppression either lack durable response to new immunosuppressive mechanisms the tumor …

Characterization And Reprogramming Of T-Cell Metabolic Dysfunction In Chronic Lymphocytic Leukemia For Improved Adoptive Cell Therapy, Wael B. Gamal

USF Tampa Graduate Theses and Dissertations

Chronic lymphocytic leukemia (CLL) is a predominant B-cell malignancy impacting elderly populations in Western societies. Targeted therapies have revolutionized the CLL treatment landscape. Still, patients often require long-term therapy and are subject to drug-induced resistance and severe toxicities, for which CLL is still considered an incurable disease. T-cell-based immunotherapy, particularly chimeric antigen receptor (CAR) T cells, has shown remarkable success, leading to various FDA approvals in hematological malignancies. Nonetheless, CAR T-cell therapy success rate is limited in CLL because of the dysfunctional nature of T cells. CLL T cells exhibit skewing toward immunosuppressive and terminally differentiated phenotypes, leading to hindrances …

Dietary Saturated Fatty Acids Modulate Host Innate Immunity, Amy Lorraine Seufert

Dissertations and Theses

Nutrition, cellular metabolism, and inflammatory regulation of the immune system are interconnected, and advancing our understanding of this will greatly improve treatment of metabolic and inflammatory disease. Specifically, dietary saturated fats are becoming increasingly appreciated for their ability to modify innate immune cell function in mammals following absorption into the blood and lymphatic circulation systems. The work herein focuses on the capacity for lipids in the diet to modulate innate immune memory. My thesis first broadly outlines fundamental background, including: the evolution of human nutrition, the discovery of innate immune memory, and key topics that reoccur throughout the text surrounding …

Perspectives Of Medical And Dental Undergraduate Students On Educational Strategies During And Post Covid-19 Lockdown, Syed Junaid Hasan, Syed Yawar Ali Abidi, Samira Adnan, Muhammad Elham Habib, Maham Muneeb Lone, Isma Sajjad, Jamshed Ahmed

Internet Journal of Allied Health Sciences and Practice

Introduction After the emergence and declaration of Covid-19 as a pandemic in March 2019, many countries implemented lockdown with cessation of essential and non-essential activities including educational and academic processes, in order to restrict the spread of the disease,. This necessitated the shift of education from face -to-face conventional methods towards online teaching and assessment practices in all domains, including medicine. This study determined the perspectives and level of satisfaction of medical and dental undergraduate students regarding the online teaching and assessment modes practiced during and after Covid-19 lockdown period. Method: The study was conducted at various medical and dental …

Addressing Social Inequalities Using Ai, Big Data, And Machine Learning, Erica L. Jensen, Lakell Archer, Sumaya Ali

Journal of Nonprofit Innovation

No abstract provided.

Feather Mites Through Genomic And Ecological Lenses: Host Specificity And Symbiosis At Multiple Levels Of Organization, Alix Elizabeth Matthews

Student Theses and Dissertations

Symbionts, or organisms that form close and persistent associations with hosts, are ubiquitous across the tree of life, and symbioses are among the most common interspecific interactions in the natural world. Symbionts play a fundamental ecological and evolutionary role across multiple levels of biological organization – from individuals to ecosystems – and can have strong effects on global biodiversity. However, many aspects of these relationships are poorly understood including symbiont genetic diversity (inter- and intraspecific), host specificity, and the mechanisms underlying their functional nature or any context-dependent patterns. This dissertation uses a model symbiosis between permanent, obligate, ectosymbiotic vane-dwelling feather …

Protocol To Construct Humanized Mice With Adult Cd34, Chun I. Yu, Richard S. Maser, Florentina Marches, Jacques Banchereau, Karolina Palucka

Faculty Research 2024

Humanized mice, defined as mice with human immune systems, have become an emerging model to study human hematopoiesis, infectious disease, and cancer. Here, we describe the techniques to generate humanized NSGF6 mice using adult human CD34 + hematopoietic stem and progenitor cells (HSPCs). We describe steps for constructing and monitoring the engraftment of humanized mice. We then detail procedures for tissue processing and immunophenotyping by flow cytometry to evaluate the multilineage hematopoietic differentiation. For complete details on the use and execution of this protocol, please refer to Yu et al.

Beyond Standard Urine Culture: Advanced Molecular Testing For Urinary Tract Infections, Rob E. Carpenter

Human Resource Development Faculty Publications and Presentations

In this review article, it is emphasized that adopting advanced molecular diagnostics is essential for modern UTI management, offering a more comprehensive, accurate, and rapid approach compared to traditional methods. Standard urine culture’s limitations include low sensitivity and failure to culture certain microorganisms, leading to undiagnosed cases and increased morbidity. Advanced molecular techniques, like multiplex-PCR and pooled antibiotic susceptibility testing have been shown to reduce empiric treatments and negative outcomes significantly. And advanced molecular methods like metagenomics (mNGS) offer comprehensive pathogen detection without prior knowledge of target organisms, improving diagnostic yield. These methods also detect antibiotic resistance genes, aiding in …

Predictors Of Hiv Knowledge, Perceived Stigma And Risk Among Transport Workers In Mbarara City, Southwestern Uganda, Benjamin Betunga, Lilian Nuwabaine, Eve Katushabe, Grace Among, Grace Nakate, Ahmed Sarki, Diana Mbattude, Mary Namuguzi, John Baptist Asiimwe

School of Nursing & Midwifery, East Africa

Background: The human immunodeficiency virus (HIV) prevalence among transport workers in sub-Saharan Africa remains high, estimated at as high as 9.9% in western Uganda compared with the national prevalence of 5.4%. The prevalence of HIV among transport workers has been partly attributed to the level of knowledge regarding HIV prevention, perceived HIV risk, and stigma. Accordingly, these have been linked to high-risk HIV transmission behaviours that increase the chances of acquiring HIV among adults. Therefore, this study investigated the predictors of HIV knowledge, perceived HIV risk, and stigma among transport workers in Mbarara city in southwestern Uganda.

Methods: The …

Group B Streptococcus Intestinal Barrier Disruption And The Protective Role Of Butyrate, Kristen Dominguez

USF Tampa Graduate Theses and Dissertations

Group B Streptococcus (Streptococcus agalactiae; GBS) is a leading cause of neonatal sepsis worldwide. Intrapartum antibiotics in GBS-colonized mothers significantly reduced the incidence of early-onset sepsis occurring in the first week of life; however, there are currently no prophylactic interventions for late-onset sepsis, occurring within the first 3 months of life. GBS is a pathobiont that primarily colonizes the gastrointestinal tract and can cross the intestinal barrier via both the paracellular and transcellular routes, causing invasive disease. Neonatal susceptibility to GBS intestinal translocation is mediated by inadequate epithelial barrier function and microbiome immaturity; however, the mechanisms by which GBS exploits …

A Case Of Reactivation Tuberculosis, Edwin Perez, Md, Avish Patel, Md, Taha Awan, Md, Amman Bhasin, Md, Gautam George, Md

The Medicine Forum

Approximately 22 percent of the world population is estimated to be infected with Mycobacterium tuberculosis. The largest number of cases are seen in Sub-Saharan Africa, India, and the islands of Southeast Asia, while North America and Western Europe are “low incidence” regions.¹ In the US, most cases are seen in foreign-born individuals who emigrate from high-incidence countries. Despite the low incidence in the US, prevention and control of Tuberculosis (TB) remain an important component of Public Health.² Here we present a case of a patient of Vietnamese origin with reactivation TB and rare manifestations of TB.

When Obstruction Meets Infection: A Case Of Multi- Drug Resistant (Mdr) E. Coli Community Acquired Pneumonia In A Patient With Metastatic Melanoma, Victor Diaz, Ms, David Saeed, Ms

The Medicine Forum

Multidrug resistant Escherichia Coli (MDR E. Coli) is a less common cause of bacterial community-acquired pneumonia as compared to Streptococcus pneumoniae and Staphylococcus aureus. We present a case of MDR E. Coli community-acquired pneumonia diagnosed on culture of bronchoalveolar lavage specimens (BAL) after bronchoscopic debulking of a metastatic melanoma mass completely obstructing the left main stem bronchus (LMSB).

Sordos, Ciegos Y Mudos, Mis .

CouRaGeouS Cuentos: A Journal of Counternarratives

No abstract provided.

Strategies For Evaluating Training For Field Engineers In The Aerospace And Defense Industry, Nathan D. Johnson

Walden Dissertations and Doctoral Studies

Inefficient training strategies are just one of the many challenges field engineer managers face in the aerospace and defense industry. Aerospace and defense managers and customers are concerned because inefficient training strategies for field engineers impact how well they can effectively contribute to organizational and customer mission success in operations around the globe. Grounded in the Kirkpatrick model, the purpose of this qualitative pragmatic inquiry was to explore strategies aerospace and defense managers use to evaluate field engineer training programs. The participants were five aerospace and defense managers who successfully implemented training evaluation strategies. Data were collected from semistructured interviews …

A Staff Education Program On The Education Of Nurses On Transgender Health Care, Diana Collins

Walden Dissertations and Doctoral Studies

In nursing education, both preregistration and continuing professional development, there is an absence of information about the specific needs of transgender people. The practicum site observed that education on the needs of the transgender community was lacking, discussing a discrepancy in the information offered and the scant attention paid to the unique requirements of transgender individuals. The goal of this project was to create a program that would raise staff members’ understanding of the exceptional health care requirements of the transgender population by providing them with an evidence-based assessment screening tool and training on transgender health care for nurses. The …

Strategies For Evaluating Training For Field Engineers In The Aerospace And Defense Industry, Nathan D. Johnson

Walden Dissertations and Doctoral Studies

Inefficient training strategies are just one of the many challenges field engineer managers face in the aerospace and defense industry. Aerospace and defense managers and customers are concerned because inefficient training strategies for field engineers impact how well they can effectively contribute to organizational and customer mission success in operations around the globe. Grounded in the Kirkpatrick model, the purpose of this qualitative pragmatic inquiry was to explore strategies aerospace and defense managers use to evaluate field engineer training programs. The participants were five aerospace and defense managers who successfully implemented training evaluation strategies. Data were collected from semistructured interviews …

A Staff Education Program On The Education Of Nurses On Transgender Health Care, Diana Collins

Walden Dissertations and Doctoral Studies

In nursing education, both preregistration and continuing professional development, there is an absence of information about the specific needs of transgender people. The practicum site observed that education on the needs of the transgender community was lacking, discussing a discrepancy in the information offered and the scant attention paid to the unique requirements of transgender individuals. The goal of this project was to create a program that would raise staff members’ understanding of the exceptional health care requirements of the transgender population by providing them with an evidence-based assessment screening tool and training on transgender health care for nurses. The …

Association Between Macrophage’S Cell Number And Maternal Factors In Human Milk, Ghaniyyatul Khudri, Dewi Sukmawati, Atikah Chalida Barasila, Dwi Anita Suryandari

Makara Journal of Science

Infant nutrition and immune protection require the consumption of human milk, with macrophages mainly contributing to immune defense and infant development. This study was performed to investigate the correlation between macrophage cell numbers in human milk and various maternal factors. Thirteen human milk samples were collected from lactating mothers and were evaluated for macrophage cell numbers using a hemacytometer. The recorded questionnaire containing several questions, including age, multivitamin consumption, coronavirus disease 2019 vaccination status, allergy history, and lactation duration, was used to determine the maternal factors. Pearson’s correlation and Mann-Whitney test was employed in statistical analysis, with p-values < 0.05 considered significant. The macrophage cell number reached 54.236 ± 7.456 cells per mL. Notably, a significant correlation was observed between maternal allergy history and the number of macrophage cells in human milk (p …