Digital Commons Network^™

Phospholipid Scramblase 1 (Plscr1) Regulates Interferon-Lambda Receptor 1 (Ifn-Λr1) And Ifn-Λ Signaling In Influenza A Virus (Iav) Infection, Alina Xiaoyu Yang, Lisa Ramos-Rodriguez, Parand Sorkhdini, Dongqin Yang, Carmelissa Norbrun, Sonoor Majid, Sanghyun Lee, Yong Zhang, Michael Holtzman, David F Boyd, Yang Zhou

2020-Current year OA Pubs

Phospholipid scramblase 1 (PLSCR1) is an interferon-stimulated gene (ISG) that has several known anti-influenza functions. However, the mechanisms in relation to its expression compartment and enzymatic activity have not been completely explored. Moreover, only limited animal models have been studied to delineate its role at the tissue level in influenza infections. Our results showed that influenza A virus (IAV)-infected

Cytomegalovirus Associated Enterocolitis And Skin Eruption: A Pediatric Case Misdiagnosed As Acute Appendicitis, Zeynep Busra Balik, Gulsen Akoglu

Journal of Pediatric Infectious Diseases

We present a case of an immunocompetent 2-year-old boy, with cytomegalovirus-associated enterocolitis and petechial skin eruption, initially misdiagnosed as acute appendicitis. Despite abdominal pain, diarrhea and a rash for 15 days, diagnostic laparoscopy revealed no surgical pathology. Dermatological examination identified perifollicular petechiae, leading to a diagnosis of cytomegalovirus infection, which was confirmed by serology. This case emphasizes the importance of considering viral infections in pediatric patients with gastrointestinal and dermatological symptoms. It also highlights the diagnostic value of cutaneous findings in cytomegalovirus infection.

Symptom Spectrum And Risk Factors For Fever In Pediatric Patients With Omicron Infection: A Prospective Observational Study, Jiande Chen, Qiuyu Tang, Dong Wang, Shuhua Yuan, Jilei Lin, Fengquan He, Yong Yin

Journal of Pediatric Infectious Diseases

Objective: The omicron variant of coronavirus disease 2019 (COVID-19) has caused a global epidemic in 2022. Our aim was to investigate the symptom spectrum and risk factors for fever in pediatric patients with omicron infection. Methods: From April 8, 2022, to May 17, 2022, this prospective observational study included pediatric patients who attended the child-parent sections of the Fangcang shelter hospitals with laboratory-confirmed COVID-19. Patient data were collected from questionnaires. The risk factors for prolonged fever and high fever were identified by univariate and multivariate analyses. Results: In total, 1,541 pediatric patients were included. The most common symptoms associated with …

Lipid Biomarkers Predict Pneumonia Risk In Children With Influenza-Mycoplasma Co-Infections: A Retrospective Cohort Study, Kai-Jing Zhang, Xin-Feng Zhao, Xiao-Juan Lv, Xiao-Hui Huang, Jing Huang, Yan-Yan Zhu

Journal of Pediatric Infectious Diseases

Objective: This study evaluates the prognostic value of high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) levels for pneumonia risk in children co-infected with influenza and Mycoplasma pneumoniae (MP). Methods: We retrospectively analyzed 248 children with influenza-MP co-infection (January 1, 2020–October 30, 2024). Participants were grouped into pneumonia (P, n=195) and non-pneumonia (NP, n=53) based on chest imaging. Statistical comparisons of clinical parameters (WBC, lymphocytes, CRP, lipids) utilized Mann-Whitney U and chi-square tests. Variables with P<0.1 were included in logistic binary regression to identify independent risk factors. ROC analysis assessed diagnostic performance. Results: Significant differences were observed in lymphocytes (L), CRP, TG, HDL-C, and LDL-C levels between pneumonia and non-pneumonia groups (P < 0.05). CRP, HDL-C, and LDL-C were identified as independent risk factors for determining whether influenza-MP co-infections were complicated by pneumonia. CRP and LDL-C levels were risk factors associated with pneumonia risk (OR > 1), whereas HDL-C levels showed a strong protective factor association (OR < 1). The ROC curve analysis showed that the combined area under the curve (AUC) for HDL-C + LDL-C (0.878) was significantly higher than the AUCs for CRP + HDL-C (0.809), CRP + LDL-C (0.753), HDL-C (0.807), LDL-C (0.737), and CRP (0.601). The clinical DCA shows that HDL-C + LDL-C has a higher diagnostic value than CRP + HDL-C, CRP + LDL-C, HDL-C, LDL-C, or CRP alone. Conclusion: HDL-C and LDL-C levels independently predict pneumonia progression in influenza-MP co-infected children, with dual-marker assessment enhancing diagnostic accuracy.

Retrospective Evaluation Of Demographic, Clinical, Laboratory Findings And Inflammatory Biomarkers Of Patients Followed With The Diagnosis Of Serum Sickness-Like Reaction, Seda Taşdemir Salar, Alaaddin Yorulmaz, Vildan Güngörer, Şükrü Arslan

Journal of Pediatric Infectious Diseases

Objectives: Serum Sickness-like Reactions (SSLR) are Type 3 hypersensitivity reactions occurring in the presence of certain antigens. Symptoms generally begin 6-21 days after the exposure to antigenic stimulation. In the present study, the purpose was to examine whether there are any changes in these hematological parameters and their prognostic significance in patients diagnosed with SSLR and followed and treated in our clinic. Methods: The files of 122 patients who were hospitalized and followed up with a diagnosis of SSLR in the Pediatric Healthcare and Diseases Clinic between September 2016 and December 2021 were examined retrospectively. Results: A total of 64 …

Application Of The Phoenix Sepsis Score For Diagnosing Sepsis In Children With Measles, Tam Thi Phung, Nguyen The Nguyen Phung, Tuan An Van Ngo, Minh Duy Vo, Hieu Trung Pham, Hai Nhi Thai Pham

Journal of Pediatric Infectious Diseases

Objective: To assess the prevalence of sepsis and septic shock in children with complicated measles using the Phoenix Sepsis Score Criteria (PSSC) during the first 24 hours of admission to the Infectious Diseases Intensive Care Unit (IDICU). Methods: This prospective observational cohort study was conducted in the IDICU at Children’s Hospital 1, Ho Chi Minh City, Vietnam, from June 2024 to December 2024. The study included 161 children aged 1 month to 16 years with measles-associated complications. Results: A total of 161 cases, with a median age of 9 months (IQR: 6–21), were included. Among them, the 49.7% were infants …

The Role Of Presepsin And Neutrophil Gelatinase-Associated Lipocalin (Ngal) Levels In Pediatric Urinary Tract Infections, Cansu Sert, Ayşe Şahin, Nazan Dalgıç, Zeynep Mine Yalçınkaya Kara

Journal of Pediatric Infectious Diseases

Objective: This study aimed to assess the utility of presepsin and neutrophil gelatinase-associated lipocalin (NGAL) levels, in serum and urine, for diagnosing urinary tract infections (UTIs) and monitoring response to treatment. By evaluating the specificity of these biomarkers for UTIs, this study sought to contribute to the existing literature. Methods: A single-center prospective study was conducted, with 32 pediatric patients diagnosed with UTIs and 32 healthy pediatric controls. Blood and urine samples were taken at the time of hospitalization and at the 72nd hour of treatment for the UTI group, and during routine clinic visits for the control group. Serum …

Lived Oral Health Experiences Of Syrian Refugees In The United States, Taniya Michael Henry-Sangar

Walden Dissertations and Doctoral Studies

The oral health status of Syrian refugees has been impacted by war and displacement since the 2011 civil war. To date, limited in-depth knowledge is available on oral health experiences of resettled Syrian refugees in the United States. The purpose of this qualitative study was to explore lived oral health experiences of resettled Syrian refugees before, during, and after the 2011 Syrian civil war. The health belief model (HBM) and theory of planned behavior (TPB) were theoretical frameworks for this phenomenological inquiry. The study was guided by semi-structured phone interviews with a purposeful sample of 11 Syrian refugees over the …

Adapter Changes Of Central Line Patients On Total Parenteral Nutrition, Courtney Erin Schappell

Walden Dissertations and Doctoral Studies

Central line-associated bloodstream infections (CLABSIs) remain a significant and preventable patient safety issue, especially among hospitalized patients receiving total parenteral nutrition (TPN). Patients on TPN face a higher risk of infection due to the high glucose content of the solution and the frequent access required for central line therapy. The Centers for Disease Control and Prevention and the Agency for Healthcare Research and Quality have recognized CLABSI prevention as a national priority. In addition, there is emphasis on high-risk groups like patients receiving TPN. The Infusion Therapy Standards of Practice, an evidence-based guideline, recommends catheter care, including timely adapter (i.e., …

Correlational Study Of Health Checkups And Patient Wellness: Epidemiological Evaluation On Prophylaxis Pathways, Samantha H. Beaubrun

Walden Dissertations and Doctoral Studies

Health checkups and wellness visits are a vital pathway to early detection and diagnosis of disease. It is not known whether young adults, insured or not, are opting into preventive care services to optimize health status via health checkups/wellness visits at primary care academic health centers. The purpose of this quantitative study was to examine the relation of wellness visits to healthcare insurance and health status while adjusting for gender among young adults ages 18–35 years in the United States. A descriptive correlational design was used with the 2023 National Health Interview Survey data set (N = 6,544). The results …

Hospital Healthcare Worker Clothes Uses Compared To The Infection Control Guidelines, Peshraw Yassin Sharif, Ramand Mohammed Haji

Hilla University College Journal For Medical Science

Background: Hospital clothing can inadvertently be a vector for harmful microbes, spreading pathogens through direct contact or airborne particles when clothes are touched or disturbed. Contaminated linens and clothing not only place patients at risk but also threaten the safety of healthcare workers. The current study compares the daily use of healthcare workers’ hospital clothes according to the infection prevention and control guidelines.

Method: A descriptive cross-sectional study was conducted on 450 healthcare staff providing medical care to patients in Raparin province, Iraq healthcare settings. The study was carried out from 4^th November 2023 to 20^th January 2024. …

Association Between Tlr2 Gene Mutations And Pulmonary Function In Patients With Pulmonary Tuberculosis, Hayder Ghali Wadi Algawwam, Basheer Ali Kareem

Hilla University College Journal For Medical Science

Background: Toll-like receptor 2 (TLR2), which is expressed on myeloid cells, detects harmful substances from invading infections or host-damaged tissues, leading to inflammation.

Objectives: Evaluation of the effects of TLR2 gene mutation on pulmonary function in pulmonary tuberculosis.

Methods: A prospective observational study lasting from January 2024 to December 2024 carried out on a sample of Iraqi patients from Kirkuk city who attended TB units at primary health care districts. Spirometry was done for all patients 2 months after the onset of anti-TB treatment and again after 6 months at the end of the treatment course. Blood samples …

Quantitative Structure-Activity Relationship, Pharmacokinetics, Drug-Likeness, Toxicity, And Molecular Docking Studies Of 4-N-(Methyl)-4-Aminoquinoline Derivatives As Antimalarial Compounds, Muhamad Jalil Baari, Devi Hardiyanti

Makara Journal of Science

Antimalarial drug resistance has encouraged various innovations to develop novel drug compounds that are effective, feasible, and safe, adhering to health standards. One way to do that is by observing and predicting the biological activity of drug compounds using quantitative structure–activity relationship (QSAR) analysis. In this study, QSAR analysis was conducted on the 4-N-(methyl)-4-aminoquinoline derivatives, which effectively inhibit the growth of Plasmodium falciparum as a source of malaria. The research stages involved molecular structure modeling, molecular geometry optimization using the AM1 semi-empirical method, and QSAR descriptor calculations, including electronic (atomic charges (q), HOMO and LUMO energies, polarizability (α), …

Perspectives On The Future Of The Profession Of Microbiology, Todd L Kelson, Heidi B Kaplan, Michelle Dziejman, Shilpa Gadwal, Irene Hulede, Jennifer Bennett, Roger Greenwell, Shelley Payne, Kyle Maclea, Eric Miller, Diana L Vullo, Rebecca Sparks-Thissen, Astral Bertolio, Jorge Cervantes

Faculty, Staff and Student Publications

The Profession of Microbiology (POM) embodies the bulk of the American Society for Microbiology (ASM) members and represents the career preparation arm of the ASM for academia, industry, and clinical lab professions. The ASM Council on Microbial Sciences hosted a virtual retreat in 2025 to identify the future of the POM. The retreat presentations centered on workforce development, professional development, innovations in technology, and interdisciplinary collaborations. Various aspects were identified, such as the need to prepare for careers in industry, as an important goal of future training. It was also clear that scientists, in all walks of life, need professional …

Advancing Skin Cancer Diagnostics: A Comparative Analysis Of Convolutional Neural Network Models For Skin Lesion Classification, Jesse Osuji, Tania Malik

SURE Journal: Science Undergraduate Research Experience Journal

For many years, skin cancer diagnosis and cancer research have been major focal points in the healthcare sector. Accurate classification of different types of skin lesions, including cancerous ones, is essential for proper diagnostics. Many machine learning (ML) techniques and models are used to solve various problems across various domains. One of such techniques is called neural networks. Convolutional Neural Networks (CNNs) are primarily used in computer vision-based problems, allowing computer systems to process and understand images. CNNs offer a potential tool for diagnosing skin lesions from images, occasionally demonstrating superior accuracy compared to trained dermatologists. This project explores various …

Developing A Tool To Measure Resistance To Sustainable Food Choices: A Pilot Study In Ireland And The Netherlands, Kate Flanagan, Bart Sinteur, Patricia J. Bulsing, John M. Kearney

SURE Journal: Science Undergraduate Research Experience Journal

Consumer uptake of a sustainable diet is critical for addressing environmental challenges and promoting public health. Understanding why consumers resist adopting sustainable eating habits can inform targeted interventions and policies. This pilot study examines whether a newly developed scale can effectively detect consumer resistance to a sustainable diet. Currently, there is a lack of research on a tool to measure consumer resistance to a sustainable diet, highlighting the need for a reliable instrument.

The aim of this study is to develop a scale, based on existing literature, to measure consumer resistance to a sustainable diet. Additionally, the study aims to …

Editorial-Sure Journal Volume 7, Issue 2, Geraldine M. Dowling, Eva Campion, Sinead Loughran, Ariane Perez-Gavilan, Josephine Treacy

SURE Journal: Science Undergraduate Research Experience Journal

No abstract provided.

Interventions To Promote Menstrual Health Among Adolescents In Lmic’S: Protocol For A Systematic Review, Precilla Dsilva, Sudhir Prabhu Dr, Prajna Kumari Dr, Baby S. Nayak Dr, Judith Noronha Dr

Manipal Journal of Nursing and Health Sciences

Background: Menarche brings out significant change in each girl’s life. Coping with the discomforts and adopting the body changes becomes a biggest challenge for every teen. Cost effective non pharmacological interventions will help to bring about improvement in the general wellbeing and quality of life in each adolescents life.

Objective: This review aims to determine the different menstrual interventions adopted by adolescent girls residing in Low and Middle Income Countries (LMIC’s). The Review question will be :What interventions are currently available, and how do they contribute in promoting menstrual health among adolescents from LMICs

Methods: This review will include …

Impact Of The Covid-19 Outbreak On The Emotional Responses And Self-Protective Behaviors Of Nursing Students., Banisha Lyngdoh Nonglait, Jyothsna Jennifer Hans, S P Sagar, Gayatri Poojari, Sanju Pant, Maria Pais Dr

Manipal Journal of Nursing and Health Sciences

Background: The COVID-19 pandemic has disrupted nearly every aspect of our society. The higher education sector is no exception. Students face an increasingly unstable environment, impacting their physical and emotional health.

Objectives: The study aims to determine students' emotional responses to the impacts of the COVID-19 outbreak. It also assesses their behavior in protecting themselves from cross-contamination during this period.

Methods: A descriptive survey design was used in this study, which included 61 undergraduate students. To collect data, researchers employed socio-demographic questionnaires alongside a structured attitude survey designed to assess emotional responses and self-protective behaviors related to COVID-19. The reliability …

Isolation And Antibacterial Evaluation Of Bioactive Compounds From Mussaenda Philippica A.Rich.: A Bioassay-Guided Approach, M. Rifqi Efendi, Mesa Sukmadani Rusdi, Muhammad Iqbal, Ilham Padavi, Sri Indah Pirmawati, Deddi Prima Putra

The Thai Journal of Pharmaceutical Sciences

Background: Mussaenda philippica A.Rich. (commonly known as Nusa Indah in Indonesia), a member of the Rubiaceae family, has been traditionally used throughout Southern Asia to treat various ailments, including bacterial infections, inflammation, ulcers, and liver disorders. These medicinal uses are particularly associated with its bark, leaves, sepals, and root. Objectives: This study aimed to isolate and identify bioactive compounds from M. philippica white modified sepals using a bioassay-guided approach and evaluate their antibacterial activity.
Materials and Methods: The study consisted of four main stages: (i) methanol extraction and liquid–liquid partitioning of M. philippica sepals; …

Prevalence Of Aminoglycoside Resistance Genes Among Esβl Producer P. Aeruginosa In Burn Cases, Yasser Rabee Ibraheem, Majida Malik Alshammari

Al-Esraa University College Journal for Medical Sciences

Pseudomonas aeruginosa recurrently develops resistant towards aminoglycosides by gaining of aminoglycoside modifying enzyme (AME) genes. The study aimed to assess the antibiotic susceptibility profile of P. aeruginosa isolated from burn infections in hospitals in Baquba, AL Najaf and Karbala. Detection of genes that mediate the production of AMEs. 175 Burn swab (110 males and 65 females) were collected then cultured on MacConkey‘s and Blood agar for primary culture, and Cetrimide agar was used to isolate and purify the bacteria and incubated aerobically at 37°C for 24 hours. Then, Vitek 2 system to validate the results. Results revealed that 70 (40%) …

Video Conversation Aids To Assist In Goals-Of-Care Discussions With Older Adults In A Medical Setting: A Systematic Review, Ashna S Karpe, Mokunfayo O Fajemisin, Stephanie Martinez Ugarte, Lara Ouellette, Martin L Blakely, Gina H Khraish, Shreyans V Sanghvi, Min J Kwak, Jessica L Lee, Lillian S Kao, Thaddeus J Puzio

Library Staff Publications

Background

Methods

Implementation Of Single-Pill Combination Medication For Hypertension Treatment By Nonphysician Health Care Workers At Primary Healthcare Facilities In Nigeria: An Explanatory Mixed Methods Study, Emmanuel I Okpetu, Chisom Obiezu-Umeh, Boni M Ale, Abigail S Baldridge, Rosemary C B Okoli, Grace J Shedul, Gabriel L Shedul, Nanna R Ripiye, Ikechukwu A Orji, Lisa R Hirschhorn, Dike B Ojji, Mark D Huffman, Hypertension Treatment In Nigeria Program Investigators

2020-Current year OA Pubs

BACKGROUND: Single-pill combination (SPC) therapy improves hypertension control; however, its implementation in primary care settings remains limited. In Nigeria, there is insufficient evidence on factors influencing SPC uptake, particularly from the perspective of healthcare workers (HCWs). This study examined the implementation of SPC medications for hypertension treatment by nonphysician HCWs at primary healthcare facilities (PHCs) in Nigeria.

METHODS: An explanatory sequential mixed methods study was conducted, building on a cluster randomized controlled trial embedded within the Hypertension Treatment in Nigeria Program. The trial compared SPC medications with free-equivalent combination therapies across 60 PHCs (January-June 2021). A subsequent qualitative component (September-December …

Society For Immunotherapy Of Cancer: Standards For Reporting Of Multiplex Immunohistochemistry/Immunofluorescence Assays (Stormi), Sam Sater, Carlo B Bifulco, Jaime Rodriguez-Canales, Joe Yeong, Guray Akturk, Michael Angelo, Carmen Ballesteros-Merino, Peter Bankhead, Subham Basu, Jorge M Blando, Saska Brajkovic, Marco Cassano, Benjamin J Chen, Ahmet F Coskun, Tricia R Cottrell, Carlos E De Andrea, Robin H Edwards, Colt Egelston, Logan L Engle, Marc S Ernstoff, Rong Fan, Michael Feldman, Bernard A Fox, Jerome Galon, Robyn Gartrell, Sacha Gnjatic, Benjamin F Green, James L Gulley, Anne Hellebust, Stephen Hewitt, Travis J Hollmann, Lucas A Horn, William J Howat, Clifford C Hoyt, Shawn M Jensen, Arutha Kulasinghe, Wiem Lassoued, Steven Lott, James Mansfield, Sebastian Marwitz, George Netto, David B Page, Edwin Parra, David L Rimm, Scott J Rodig, Roberto Salgado, Denis Schapiro, Kurt A Schalper, Joel C Sunshine, Michael J Surace, Alexander S Szalay, Magdalena Thurin, Jose C Villasboas, Keith Wharton, Ignacio I Wistuba, Jennifer H Yearley, Yinyin Yuan, Geroge Zaki, James Ziai, Janis M Taube

Faculty, Staff and Student Publications

Multiplex immunofluorescence and immunohistochemistry (mIF/IHC) are increasingly employed antibody-based technologies that use tissue sparingly and facilitate the detection of co-localized or neighboring biomarkers. Specifically, these platforms enable spatial analyses of the tumor microenvironment as well as extended applications, for example, describing normal tissue anatomy, autoimmunity, infectious diseases, etc. mIF/IHC has greatly enhanced biomarker discovery efforts, and a growing number of studies suggest superiority to traditional IHC. Standardization of staining approaches, reporting of image analysis strategies and resultant data is critical for facilitating cross-study comparisons, validation, deployment, and generalization of findings. To address this challenge, The Society for Immunotherapy of Cancer …

Impact Of Community Health Needs Assessments As Barriers To Chronic Disease Prevention: A Comparative Study, Marissa E. Woelm Ms.

Honors Thesis

Community health needs assessments (CHNAs) are vital tools for identifying population health priorities through monitoring chronic diseases and conditions; however, their effectiveness in preventing chronic diseases remains debated. This comparative study examines CHNAs from sixteen South Dakota hospitals across Avera, Sanford, Monument Health, and independent systems to evaluate their role in preventing chronic diseases. Analysis reveals significant challenges, including biased data sampling, limited community engagement, small survey samples, and inconsistent implementation strategies. While CHNAs effectively identify mental health, cancer, and obesity as the top community concerns alongside several other diseases like diabetes and kidney disease, most reports lack specific timelines …

Updating The Epidemiology Of Blastomycosis And Histoplasmosis In The United States, Using National Electronic Health Record Data, 2013-2023., Juliana G E Bartels, Simon K. Camponuri, Theo T. Snow, Brittany L Morgan Bustamante, Natalie J. Kane, Rose Reynolds, Aidan Lee, Mark A. Hoffman, Theodore C. White, Justin V. Remais, Jennifer R. Head

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Where surveillance data are limited, nationally representative electronic health records allow for geographic, temporal, and demographic characterization of the fungal diseases blastomycosis and histoplasmosis.

METHODS: We identified incident blastomycosis and histoplasmosis cases from 2013 to 2023 within Oracle EHR Real-World Data, which comprises 1.6 billion healthcare encounters nationally. To characterize spatiotemporal incidence trends, we used generalized estimating equations weighted for nonrepresentativeness of electronic health record-reporting facilities. We computed standardized incidence rate ratios (sIRRs), which relay relative differences in standardized incidence rates between regions, race/ethnicity, gender, and age subgroups and the national population.

RESULTS: National incidence rates in 2023 were …

Development Of A Procedure For Prion Surveillance In The Laboratory Setting, Damian Gorski, Isaac Schauer, Kane Spicker, Ananya Tupaki-Sreepurna, Fei Wang, Claudio Soto, Sandra Pritzkow

Faculty, Staff and Student Publications

Infectious prions readily adhere to common surfaces, retain infectivity, and are highly resistant to conventional decontamination, posing significant biosafety challenges in the medical and research environments. Recent occupational exposures underscore the urgency of improving safety measures. Here, we describe an approach combining foam-swab surface sampling and protein misfolding cyclic amplification to enhance prion surveillance. Our results demonstrate the ability to detect prions most relevant to human health directly from contaminated surfaces, even at 100 million-fold dilutions of the brain. We applied our method to assess the completeness of prion decontamination and show that high prion quantities can resist even approved …

The Common Cold Is Associated With Protection From Sars-Cov-2 Infections, Camille M Moore, Katherine Rivera-Spoljaric, Et Al.

2020-Current year OA Pubs

BACKGROUND: Adults and children often respond differently to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, with adults facing a higher risk of symptomatic and severe illness. We hypothesize that children's protection from symptomatic SARS-CoV-2 may be due to more frequent respiratory viral infections, which prime their airway antiviral defenses.

METHODS: Using case-cohort and case-control analyses in the Human Epidemiology and Response to SARS-CoV-2 cohort, we evaluated whether infection with common respiratory viruses protects against SARS-CoV-2 infections and investigated airway molecular mechanisms by which this protection is achieved. We tested 10 493 longitudinal nasal swabs from 1156 participants for 21 …

Just "Beet" It? A Comparison Of Road Salt Effects On The Life History Of Daphnia Magna, Koby Sellner

The Journal of Purdue Undergraduate Research

In areas that experience snow and ice, deicing reagents such as road salts are often used on roads to promote safer driving conditions. However, road salts run off into local vegetation, soil systems, and (my focus) freshwater systems. The impacts of excess salt and chloride in freshwater have been studied extensively on a broad diversity of organisms under different conditions, but previous studies have rarely used different types of road salts. Zooplankton are primary consumers in many freshwater systems, feeding on phytoplankton and serving as prey for predator species higher in the trophic chain. These animals generally are impacted negatively …

The Automation Of Lamp Biomolecular Assays Using The Eppendorf Epmotion™ 5075 Robotic Pipettor, Shamalaa Sathiasealan

The Journal of Purdue Undergraduate Research

Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique known for its specificity and efficiency in less controlled environments than laboratories. Designing novel LAMP reagents requires strict contamination control, and unlike the more commonly automated polymerase chain reaction, LAMP’s heightened sensitivity poses unique challenges to automation. To address this, we optimized automation procedures for LAMP using a robotic pipettor without altering the core assay itself. This project focuses on minimizing contamination using sealed plates, reducing operator intervention by automating pipetting, and preventing enzyme degradation using cooling plates. Our approach employs pre-slit pierceable seals and cooling blocks to prevent cross-contamination …