Digital Commons Network^™

Influence Of Lactobacillus Rhamnosus Isolated From “Amabere Amaruranu” Cultured Milk On Adipogenesis, Justin E. Kotala

Electronic Theses and Dissertations

This study was performed to test the in vitro effects of a Lactobacillus rhamnosus isolate from “amabere amaruranu”, a traditional Kenyan cultured milk, on 3T3-L1 and Caco-2 cell lines. Cultures of fully mature 3T3-L1 adipocytes were treated with bacterial isolate cell extract (CE), filtered spent broth (FSB) from overnight bacterial culture, or with a PBS control. Expression levels of PPAR³1 and 2, C/EBP±, and ATGL proteins in 3T3-L1 cells were upregulated by FSB treatment. CE treatment did not affect protein expression levels. Expression of MTTP and SREBP-1c proteins in Caco-2 cells showed no change with either treatment. Optical density measurements …

Optimization Of Lead Spectinamide Compounds As Novel Anti-Tuberculosis Agents With A Pharmacometric Approach, Ashit Rasendu Trivedi

Theses and Dissertations (ETD)

In an effort to combat the global Tuberculosis pandemic, Dr.Richard E. Lee and his group at St.Jude Children’s Research Hospital designed a novel series of anti-tuberculosis agents, spectinamides – semi-synthetic analogs of spectinomycin. Spectinamides are a potent inhibitor of mycobacterial ribosomes and overcome efflux mediated drug resistance in M. tb. Spectinamides have shown an excellent in vitro activity, which makes them well suited for further lead optimization and preclinical development. We hypothesized that through pharmacokinetic (PK) and pharmacodynamics (PD) model-based dosing optimization studies, we could strategically guide the selection and refinement of more potent and effective anti-TB spectinamides. Biopharmaceutical in …

Screening Bioactive Molecules In Tetrahymena Thermophila Using Phagocytosis Determined By Flow Cytometry, Santosh Gummidipundi

Master's Theses

The evaluation of molecules that have potentially bioactive significance is an expanding field, but current screening assays are expensive and time consuming. Mammalian cell-based assays require aseptic techniques, complex media and long generation times. This thesis reports on an assay that incorporates Tetrahymena phagocytic ingestion and flow cytometry to evaluate several potentially bioactive molecules. Several factors that influenced optimal and reproducible results were analyzed. Seven potentially bioactive molecules (serotonin, norepinephrine, dopamine, morphine, colchicine, mexiletine and procainamide) were evaluated to determine their effect on phagocytosis by Tetrahymena. Assay results indicated that the phagocytic rate was unaffected by serotonin, norepinephrine, dopamine, and …

The Role Of Nitric Oxide In Skeletal Simulated Microgravity Response, Luan Tran

Master's Theses

Prolonged exposure to the space environment leads to bone loss. Using a ground-based model for microgravity (hind-limb unloading), it has been shown in rodents that proliferation and differentiation activity of osteoprogenitors are decreased with exposure to simulated microgravity (Kostenuik et al., 1997). Osteoblast mineralization activity has been shown to increase with nitric oxide supplementation (Afzal et al., 2004; Koyama et al., 2000). I hypothesized that the degenerative effects of microgravity exposure result from chronic changes in nitric oxide regulation in osteoprogenitors, and that supplementation with exogenous nitric oxide following exposure would ameliorate these changes. In 20 C57Bl/6 mice, hind-limb unloading …

Efficacy Of Galactooliosaccharide (Gos) And/Or Rhamnose-Based Synbiotics In Enhancing Ecological Performance Of Lactobacillus Reuteri In The Human Gut And Characterization Of Its Gos Metabolic System, Monchaya Rattanaprasert

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Probiotic L. reuteri has potential to produce antimicrobial compounds and secrete immunosuppressive factors. These metabolic attributes could benefit the human host by providing colonization resistance (competitive and metabolic exclusion) against enteropathogens and mitigating inflammation. As metabolically active cells are fundamental to such probiotic properties, synbiotic approaches that supply L. reuteri with a source(s) of carbon, energy, and/or external electron acceptor for cell growth in the gut environment could therefore prompt the probiotic to engage in beneficial activities. In this study, the efficacy of GOS and/or rhamnose-based synbiotic approaches in promoting colonization persistence and metabolic activity of L. reuteri was evaluated. …

Context Construction Through Material Perceptions: Experiences From An Explorative Workshop, Riikka Townsend, Salu Ylirisku

Experiential Knowledge (EKSIG) Conference Series

This paper describes and reflects upon a design-led workshop carried out by design and science researchers in an interdisciplinary material development project. The aim of the workshop was to construct application context for the design of thermoelectric energy harvesting applications that combine textiles, thermoelectric materials, electronics, and additional smart materials. The experience of textiles is typically multi-sensorial as textiles are often close to the body or in the touching proximity to people. However, the development of novel textile materials and applications is often science-led resulting in extensive considerations of technical aspects at the cost of the experiential insights. The workshop …

Assessing A Monitoring Scale Of Physiological Health And Risk Assessment Among Those Exposed To Heated Environments: A Brief Report, Corey A. Peacock, Ellen L. Glickman, Gabriel J. Sanders, Yong Suk Seo, Brandon S. Pollock, Keith J. Burns, Lynn Kakos, John Gunstad

Journal of Human Performance in Extreme Environments

Background: Prevention of heat illness is of considerable medical interest within the field of occupational work. There are many established methods of perceptual health assessment; however, many are rather unpractical and timely. The objective was to improve the practicality and timeliness of perceptual physiological monitoring; a Heat Thermal Sensation scale has been developed. The usefulness of the scale was assessed on its ability to monitor physiological variable. Materials and Methods: Ten apparently healthy individuals performed physically exerting activity while exposed to 37 °C. Sensation and physiology were measured throughout. Results: The perceptual monitoring scale demonstrated weak positive correlations with human …

Safe Operations Above Fl600, Lt. Col. Stephen Hunter

Space Traffic Management Conference

Safe Operations Above FL600

Abstract

With the increase in likelihood of near-term development of revenue-generating point-to-point suborbital flights and the increase in high-altitude-long-endurance commercial operations above FL600, the criticality of addressing operations above class A airspace has never been more pressing. While the Federal Aviation Administration describes the National Airspace System, it doesn’t offer a description that includes a top. Technological, physiological, physical and administrative limitations have relegated most air operations to FL600 and below. As that changes, previous work on Space Traffic Management (STM) concepts begin to take on new life and add to sincere considerations for adaptation or …

Prediction: The Quintessential Model Validation Test, Wayne Wakeland

Systems Science Friday Noon Seminar Series

It is essential to objectively test how well policy models predict real world behavior. The method used to support this assertion involves the review of three SD policy models emphasizing the degree to which the model was able to fit the historical outcome data and how well model-predicted outcomes matched real world outcomes as they unfolded. Findings indicate that while historical model agreement is a favorable indication of model validity, the act of making predictions without knowing the actual data, and comparing these predictions to actual data, can reveal model weaknesses that might be overlooked when all of the available …

Caribou, Petroleum, And The Limits Of Locality In The Canada–Us Borderlands, Jenny Kerber

English and Film Studies Faculty Publications

his article discusses Karsten Heuer’s 2006 book Being Caribou in light of debates in ecocriticism and border studies about how to define the local in the context of environmental problems of vast range and uncertain temporality. It explores how Heuer’s book about following the Porcupine Caribou herd’s migration engages in multiple forms of boundary crossing—between countries, between hemispheric locations, and between species—and shows how insights from Indigenous storytelling complicate the book’s appeal to environmentalist readers by asserting a prior, transnational Indigenous presence in the transboundary landscapes of present-day Alaska and the Yukon.

The Flaws And Human Harms Of Animal Experimentation, Aysha Akhtar

Experimentation Collection

Nonhuman animal (“animal”) experimentation is typically defended by arguments that it is reliable, that animals provide sufficiently good models of human biology and diseases to yield relevant information, and that, consequently, its use provides major human health benefits. I demonstrate that a growing body of scientific literature critically assessing the validity of animal experimentation generally (and animal modeling specifically) raises important concerns about its reliability and predictive value for human outcomes and for understanding human physiology. The unreliability of animal experimentation across a wide range of areas undermines scientific arguments in favor of the practice. Additionally, I show how animal …

The Flaws And Human Harms Of Animal Experimentation, Aysha Akhtar

Morality and Ethics of Animal Experimentation Collection

Nonhuman animal (“animal”) experimentation is typically defended by arguments that it is reliable, that animals provide sufficiently good models of human biology and diseases to yield relevant information, and that, consequently, its use provides major human health benefits. I demonstrate that a growing body of scientific literature critically assessing the validity of animal experimentation generally (and animal modeling specifically) raises important concerns about its reliability and predictive value for human outcomes and for understanding human physiology. The unreliability of animal experimentation across a wide range of areas undermines scientific arguments in favor of the practice. Additionally, I show how animal …

We Are… Bridging Medicine And Science, Vol. 1, Issue 4, Fall 2015, Marshall University Biomedical Sciences

We Are... Bridging Medicine Science

No abstract provided.

The Fight Master, Fall 2015, Vol. 37 Issue 2, The Society Of American Fight Directors

Fight Master Magazine

No abstract provided.

Focus On Boise State University, Sherry Squires (Editor)

FOCUS on Boise State

No abstract provided.

Recognizing Occupational Stress: A Case Study Of Admission Staff In A For-Profit Nursing College, Carie L. Powers

All Theses And Dissertations

While numerous studies have looked at the influence of occupational stress on employees, less is known about the effects on employees in the for-profit higher education industry. Admissions advisors in particular must endure cyclical pressures to fill classes to ensure a college’s financial viability. A criterion sample of 12 admissions advisors employed at a for-profit nursing college with campuses located throughout the United States were interviewed. The participant criterion sample specifically sought to include advisors who enroll pre-licensure nursing degree-seeking students.

The purpose of the study was to acquire a richer understanding of nursing admissions advisors’ convergence of personal recognition …

University Of Nebraska At Omaha Biomechanics Annual Report, Fall 2015, Biomechanics Research Building

Biomechanics Annual Report

This annual report includes:

Letter from the Director; B.S. in Biomechanics; QLI Partnership

Center for Research in Human Movement Variability: Formation of the Center; Breathing and Walking Coupling Variability in COPD; Gait Variability in Patients with Peripheral Arterial Disease; Development of Postural Control Variability in Autism; The Effects of Virtual Reality on Gait Variability After Stroke; Pilot Grants; COBRE Research Day; New Hires in the Center

Updates: Why Choose the BRB? Where Are They Now? From the Bench to the Market: New Partnership Paves the way to Pre-Symptomatic Disease Detection; Fun Facts; New Quipment

Other Projects: Space Navigation; Neuroscience; Prosthetics; …

Seeing Your Face Is Not Enough: An Inertial Sensor-Based Liveness Detection For Face Authentication, Yan Li, Yingjiu Li, Qiang Yan, Hancong Kong, Robert H. Deng

Research Collection School Of Computing and Information Systems

Leveraging built-in cameras on smartphones and tablets, face authentication provides an attractive alternative of legacy passwords due to its memory-less authentication process. However, it has an intrinsic vulnerability against the media-based facial forgery (MFF) where adversaries use photos/videos containing victims' faces to circumvent face authentication systems. In this paper, we propose FaceLive, a practical and robust liveness detection mechanism to strengthen the face authentication on mobile devices in fighting the MFF-based attacks. FaceLive detects the MFF-based attacks by measuring the consistency between device movement data from the inertial sensors and the head pose changes from the facial video captured by …

Integrating Meal And Exercise Into Personalized Glucoregulation Models: Metabolic Dynamics And Diabetic Athletes, Sofie Schunk

Master's Theses (2009 -)

Diabetes affects nearly 26 million Americans, according to the American Diabetes Association, with as many as three million Americans who have Type 1 Diabetes (ADA, 2015). Type 1 Diabetes (T1D) is autoimmune and characterized by little to no insulin production whereas Type 2 Diabetes (T2D) concerns insulin resistance and inability to use produced insulin. Factors contributing to current diabetes management and regulation include exercise type, daily movement activities, and distinct tissue compartment metabolism, each challenging to model in a robust and comprehensive manner. Past models are highly limited in regard to exercise and varying glucose fluctuations dependent on type, intensity, …

Characterization Of Inhibitors Of Fatty Acid Transport Protein-2 In Cell And Animal Models, Nipun Saini

Department of Biochemistry: Dissertations, Theses, and Student Research

Obesity is correlated with insulin resistance and elevated levels of glucose, triglycerides and free fatty acids in blood. This affects overall metabolism and leads to disease. In the obese state, fat also accumulates in non-adipose tissue including liver, muscle and pancreas, where it can lead to cellular dysfunction and death. Currently, only a limited number of drugs are available to combat obesity and it is clear that new drugs, which more narrowly target the metabolic pathways involved, are required. Fatty Acid Transport Proteins (FATPs) are bifunctional proteins involved in the uptake and activation of fatty acids by esterification with coenzyme …

Learning Opportunities 2015/2016, Illinois Mathematics And Science Academy

Course Catalogs

The graduation requirements of the Illinois Mathematics and Science Academy are in concert with those maintained by the State of Illinois with additional requirements as established by the IMSA Board of Trustees. Each semester students must take a minimum of 5 academic courses (2.5 credits) for a grade (not Pass/Fail). Fine Arts, Wellness, and Independent Study courses, or any course taken on a Pass/Fail basis do not count towards the 5 course (2.5 credits) minimum. Most students will take between 5 (2.5 credits) and 7 (3.5 credits) academic courses per semester. Only courses taken for a letter grade will count …

Oswald Research And Creativity Program, Uk Office Of Undergraduate Research

Kaleidoscope

No abstract provided.

The Perfect Machine: The Reason Behind The Anatomical Studies Of Leonardo Da Vinci, Amanda M. Cothern

Kaleidoscope

The legacy of Leonardo da Vinci is most often characterized by the works of his brush — however, there is more to Leonardo than what meets the art lover’s eye. His notebooks overflow with scientific studies, the most amazing of which are his detailed drawings of human anatomy. Scholars have long assumed that Leonardo dissected corpses in order to better represent the human form in his painting. In this paper, I counter that assumption, making the following points:

1. Leonardo’s anatomical findings did not significantly influence his painting.
2. Leonardo was an accomplished scientist and engineer.
3. Leonardo applied his knowledge of physics …

Biology Discipline Program Assessment 2015, Biology Discipline

Assessment of Student Learning Reports

No abstract provided.

Oleogustus: The Unique Taste Of Fat, Cordelia A. Running, Bruce A. Craig, Richard D. Mattes

Department of Nutrition Science Faculty Publications

Considerable mechanistic data indicate there may be a sixth basic taste: fat. However, evidence demonstrating that the sensation of non-esterified fatty acids (the proposed stimuli for “fat taste”) differs qualitatively from other tastes is lacking. Using perceptual mapping, we demonstrate that medium and long-chain non-esterified fatty acids have a taste sensation that is distinct from other basic tastes (sweet, sour, salty, and bitter). While some overlap was observed between these NEFA and umami taste, this overlap is likely due to unfamiliarity with umami sensations rather than true similarity. Shorter chain fatty acids stimulate a sensation similar to sour, but as …

Emergency Management In Maritime Mass Evacuation Operations - The Case Of The High Arctic, Odd Jarl Borch, Natalia Andreassen, Svetlana Kuznetsova, Sergey Markov

ShipArc 2015 Conference

No abstract provided.

Improving Antibiotic Resistant Infection Transmission Situational Awareness In Enclosed Facilities With A Novel Graphical User Interface For Tactical Biosurveillance, Valeriya V. Kettelhut

Theses & Dissertations

Serious challenges associated with antibiotic resistant infections (ABRIs) force healthcare practitioners (HCP) to seek innovative approaches that will slow the emergence of new ABRIs and prevent their spread. It was realized that traditional approaches to infection prevention based on education, retrospective reports, and biosurveillance often fail to ensure reliable compliance with infection prevention guidelines and real-time problem solving. The objective of this original research was to develop and test the conceptual design of a situational awareness (SA)-oriented information system for coping with healthcare-associated infection transmission.

Constantly changing patterns in spatial distribution of patients, prevalence of infectious cases, clustering of contacts, …

A Brief Overview Of Amblyaudia, Raelynn Lamminen, Daniel Houlihan

Psychology Department Publications

Amblyaudia, a recent subcategory of auditory processing disorder, is characterized by asymmetrical auditory processing of an individual’s ears. Amblyaudia can result in speech comprehension difficulties, reading difficulties, information processing deficits, and inattention. These difficulties can be mistakenly attributed to Attention Deficit Hyperactivity Disorder (ADHD), Specific Learning Disorders (SLD), depression, anxiety disorders, and communication disorders. Unfortunately, traditional hearing tests do not place the two ears in competition and cannot detect asymmetry. Therefore, students who exhibit these difficulties and have normal performance on traditional hearing tests should be also evaluated for amblyaudia with dichotic listening tests. Amblyaudia can be addressed through dichotic …

Quantitative Approaches For Deconvolving The Multiple Contributions Of Primary Structure To Protein Fitness, Loretta Au

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Proteins from the same family often share many structural and functional motifs. Variations in primary structure, however, allow a single protein family to modulate a broad range of biological processes. Several methods can be used to identify sequence conservation, but complementary mutagenesis experiments are often needed to understand the multiple roles that a given amino acid may have in maintaining overall fitness. Limitations related to financial and temporal costs generally constrain these experiments to smaller proteins or peptides, or to only partial sampling of sequence spaces, and we have devised a computational protocol for large-scale mutagenesis to circumvent these obstacles …

Nutrinews, Volume 8, August, 2015, Department Of Nutrition And Dietetics, University Of North Florida

NutriNews

This issue features Dementia or B Vitamin Deficiency?, Food Science News, Fruit of the Month Club: Featuring Guava