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Rabbit Breeding And Management: A Guide For Producers, Joseph Okoh, Jessie Hadfield

All Current Publications

This fact sheet provides essential information to help rabbit producers understand effective breeding goals and programs for managing and improving rabbit production.

Novel Piperidone And Bispidine Derivatives: A New Approach In Cancer Therapy, Lereen Khaled Youssry

Theses and Dissertations

Cancer remains the second leading cause of death worldwide. As a result, scientists have been trying to find novel therapeutic approaches that target specific metabolic pathways selective to tumor cells. In this study, two novel piperidone and bispidine derivatives, organic molecules that target polyamine metabolism, were synthesized, and their potential cytotoxic, antioxidant and anti-inflammatory effects were investigated. Polyamines are naturally occurring metabolites that were reported to be in excess at cancer cells and were accounted for tumor proliferation, invasion and metastasis. Accordingly, the novel piperidone and bispidine derivatives were expected to disrupt tumor growth and progression via up-regulation of key …

Innovation And Risk Management In High-Risk Medical Device Development_ Lessons From Japanese Venture Companies.Docx.Pdf, Seiya Shimanuki, Motohiro Kita, Desmond Cherng En Lee, Ahmad Massu Ramli

Jesselton Journal of Educators and Scholars

This article investigates the unique challenges and strategic responses associated with developing high-risk medical devices, emphasizing insights from the Japanese medical technology sector. Utilizing a comprehensive case study of Sun Medical Technology Institute, the research highlights critical strategies, including technological diversity management, simplification of device structures, adoption of low-tech solutions, and leveraging strategic external collaborations to mitigate developmental risks and resource constraints. Additionally, the study explores regulatory complexities, underscoring the importance of proactive compliance strategies in navigating stringent regulatory frameworks effectively. By integrating quantitative patent data analyses, including Lorenz curves and Gini coefficients, the findings reveal the significant impact of …

Workforce Resilience And Staff Retention Strategies At The Front Of House (Foh) Department: A Case Study Of Ascott Hotel Kuala Lumpur.Pdf, Hanley Chew, Jamalludin Helmi Hashim, Sie Ling Tan

Jesselton Journal of Educators and Scholars

The hospitality industry faces significant challenges in workforce resilience and staff retention, particularly in the Front of House (FOH) department, where high employee turnover can impact service quality and operational efficiency. The research offers detailed insight into workforce resilience along with staff retention strategies at the FOH department highlighting Ascott Hotel Kuala Lumpur. An overview of the Hospitality Industry has been provided for a better understanding of the topic. It also includes various research techniques along with methods considering ethical norms and rules. This study illustrates the potential of Ascott Hotel’s employees in developing workforce resilience and staff retention strategies. …

Ai In Higher Education: Balancing Innovation And Ethical Challenges.Pdf, Takahiro Fujimoto, Nelson Zee Yang Wong, Siti Aishah Berhan

Jesselton Journal of Educators and Scholars

This study examines the integration of Artificial Intelligence (AI) in higher education, focusing on both its transformative potential and the challenges it introduces for teaching, learning, and institutional operations. As AI technologies such as machine learning, natural language processing, and data analytics become more prevalent, they offer opportunities to personalize learning, enhance administrative efficiency, and support student success. However, the adoption of AI also presents significant barriers, including technological limitations, faculty resistance, ethical concerns, and disparities in access among students and institutions. Using a qualitative research approach and thematic analysis, this research explores the perspectives of administrators, faculty, and students …

An Empirical Study On Lecturers’ Perceptions Of Digital Gender Equality In Malaysia.Pdf, Fung Lan Yong, Louis Yuk Su Chin

Jesselton Journal of Educators and Scholars

The purpose of this study was to examine lecturers’ perceptions of digital gender equality in Malaysia. A Likert-scale questionnaire was designed to measure perceptions of digital gender equality and was administered to 55 lecturers online. Data were analyzed using SPSS 26.0. First, Mann-Whitney U test revealed that there were no significant gender differences in lecturers’ perceptions of digital gender equality, while Kruskal-Wallis H test indicated no significant differences in relation to age and job experience. Wilcoxon signed rank test showed that four items were significant at p < 0.05, while another eight items were significant at p < 0.001. The overall group mean showed that lecturers’ perceptions of digital gender equality were low, indicating that the majority failed to notice the impediments that women face in the digital era. Overall percentages indicated that lecturers held unrealistic views of women’s participation in the digital economy probably because they lacked knowledge on the disproportionate numbers of women who are still living in the agrarian areas. In light of the findings, several recommendations were made to increase digital gender equality in Malaysia.

The Influence Of Coca-Cola Brands On Consumer Purchases In The Beverage Market_ Japan_S Perspective.Pdf, Ryo Nishizaki, Desmond Cherng En Lee, Ahmad Massu Ramli

Jesselton Journal of Educators and Scholars

This study examines the impact of Coca-Cola’s branding on consumer purchasing behavior in Japan’s beverage market. Employing a thematic qualitative approach, the research investigates brand recognition, cultural influences on marketing strategies, product differentiation, and consumer attitudes shaped by cultural values. Findings reveal that Coca-Cola’s global brand awareness combined with localized marketing efforts, including culturally relevant campaigns and health-oriented products, significantly influence Japanese consumer preferences. However, challenges remain due to increasing health consciousness and competitive market dynamics. The study contributes to understanding how multinational brands adapt to culturally distinct markets and offers practical recommendations for enhancing brand engagement in Japan. These …

Empowering Leadership, Organizational Culture, And Lecturer Innovativeness In Malaysian Universities_ The Moderating Role Of Psychological Capital.Pdf, Shu Ya Guo, Fung Lan Yong

Jesselton Journal of Educators and Scholars

This study investigates the impact of empowering leadership and organizational culture on lecturer innovativeness in Malaysian universities, emphasizing the moderating role of psychological capital. Utilizing a comprehensive theoretical framework that integrates leadership, organizational behavior, and positive psychology theories, the research explores how leadership practices and cultural factors within educational institutions contribute to innovation among lecturers. Data were collected through surveys and analyzed using statistical methods, revealing that both empowering leadership and a supportive organizational culture significantly enhance lecturer innovativeness. Furthermore, psychological capital, encompassing self-efficacy, optimism, hope, and resilience, was found to positively moderate the relationship between leadership, culture, and innovativeness. …

Digital Transformation In Japanese Companies_ Navigating Cultural Barriers, Leadership, And Innovation In The Era Of Society 5.0.Pdf, Hideto Tsuchiya, Hoong Tat Teh, Sook Han Wong

Jesselton Journal of Educators and Scholars

This article examines the impact of digital transformation in Japanese companies, analyzing how technological, cultural, and organizational factors interact to shape adoption outcomes. Drawing on qualitative thematic analysis of interviews and case studies, the study highlights that while advanced technologies such as AI, cloud computing, and IoT offer significant potential for innovation and operational gains, their integration is often hindered by entrenched hierarchical structures, cultural resistance, and a shortage of digital skills. Government policies, including Society 5.0, have provided impetus for change, particularly among large corporations, yet many small and medium-sized enterprises continue to face challenges related to legacy systems …

Early Wound Dressing (Soft Gauze/Tape Dressing) Removal After Cesarean Delivery: A Meta-Analysis Of Randomized Trials, Leshae Cenac, Serena Guerra, Alicia Huckaby, Gabriele Saccone, Vincenzo Berghella

Department of Obstetrics and Gynecology Faculty Papers

OBJECTIVE: To assess whether removing the wound dressing within 24 hours after a cesarean delivery leads to any negative effects on wound healing and to recommend the best time for dressing removal.

DATA SOURCES: Science Direct, PubMed, Cochrane Library, and Scopus as the electronic databases from their inception until December 2024.

CRITERIA FOR STUDY ELIGIBILITY: Randomized controlled trials (RCTs) were incorporated in the databases, irrespective of their publication language, sample size, publication date, use of blinding, or study setting/location.

STUDY APPRAISAL AND SYNTHESIS METHODS: A systematic review of RCTs was conducted to compare wound outcomes in cesarean delivery patients with …

Appreciating Dissonance: Using Open Appreciative Inquiry As A Tool To Generate Cultural Change, Charles Dorr, Sheena Asthana, Julian Elston, Felix Gradinger, Daniel Preece, David Schwartz, Gemma Scott, Gary Wallace, Ruth Harrell

More Faculty of Health Research

Appreciative inquiry has become increasingly popular as a method for facilitating organisational or systemic change through focusing on the positive aspects and ‘life giving properties of a system’ as opposed to traditional ‘deficit-based’ approaches. However, there has been criticism that this process could invalidate negative experiences of organisations and/or systems. This is particularly problematic if an existing system is seen by its users as providing little value or if there is potential dissonance regarding system value among key stakeholders. Using two case studies of open appreciative inquiry by Plymouth City Council staff, this article outlines how the use of a …

Home-Based High-Intensity Interval Training For People With Parkinson's: A Randomized, Controlled, Feasibility Trial, Conrad Harpham, Hilary Gunn, Jonathan Marsden, Raul Bescos-Garcia, Christopher Murgatroyd, Luke Connolly

School of Health Professions

Background and Aims: Home-based high-intensity interval training (HIIT) could be feasible and useful for people with Parkinson's (PwP). However, no home-based HIIT program for PwP has been undertaken. This trial was designed to obtain preliminary data regarding the feasibility, acceptability and safety of HIIT-Home4Parkinson's (HH4P), a previously co-created home-based HIIT program for PwP, explore outcomes that may be sensitive to change, and inform the implementation of a potential full trial. Methods: A randomized, controlled feasibility trial was undertaken. Thirteen independently mobile PwP of Hoehn and Yahr stages 1–3 were randomized to the 12-week, three times weekly HH4P HIIT program (n …

The Feasibility Of Passively Tracking Children’S Tv Viewing And Mobile Device Use In Naturalistic Settings, Teresia M O'Connor, Tatyana Garza, Uzair Alam, Anil Kumar Vadathya, Jennette P Moreno, Alicia Beltran, Samah Haidar, Nimah Haidar, Sheryl O Hughes, Debbe Thompson, Salma M A Musaad, Tom Baranowski, Jason A Mendoza, Joseph Young, Akane Sano, Ashok Veeraraghavan

Faculty, Staff and Students Publications

Research on children's technology and digital media (TDM) is hampered by a lack of robust approaches for assessing TDM use. This study assessed the feasibility of passively measuring children's TV screens and mobile devices (TDM) in a naturalistic setting. In the three-day feasibility study, FLASH-TV was set up on one to two TVs the child (5-12 year olds) typically used in the home (n=20). Children's mobile device use was assessed with either the Chronicle App or ScreenTime screenshots. Parents completed three TDM diaries. An exit interview with the parent explored their perceptions of the assessments and the child's TDM use …

Conceptualizing The Research Cycle For The Field Of Materials Science And Engineering, Jennifer L.W. Carter

Faculty Scholarship

Abstract: Early-career materials researchers often receive guidance from their individual advisors on best practices for conducting research, but the materials science community lacks an explicit model of the research process. As a result, the lived experience of an individual researcher can be quite different from their peers since they may be exposed to a different unique set of implicit research steps. In this article, we translate an existing research heuristic from other fields and make explicit the steps, methodologies, and strategies materials science engineering researchers utilize to advance our collective materials science knowledge. This newly proposed research cycle can improve …

Exogenous Arginine Differentially Regulates Inflammatory Cytokine And Inducible Nitric Oxide Synthase Expression In Macrophages, Kelsey Stayer, Saliha Pathan, Aalekhya Biswas, Huiqiao Li, Yi Zhu, Fong Wilson Lam, Juan Marini, Sundararajah Thevananther

Faculty, Staff and Students Publications

Immune dysfunction and late mortality from multiorgan failure are hallmarks of severe sepsis. Arginine, a semi-essential amino acid important for protein synthesis, immune response, and circulatory regulation, is deficient in sepsis. However, arginine supplementation in sepsis remains controversial due to the potential to upregulate inducible nitric oxide synthase (iNOS)-mediated excessive nitric oxide (NO) generation in macrophages, leading to vasodilation and hemodynamic catastrophe. Citrulline supplementation has been considered an alternative to replenishing arginine via de novo synthesis, orchestrated by argininosuccinate synthase 1 (ASS1) and argininosuccinate lyase (ASL). However, the functional relevance of the ASS1-ASL pathway in macrophages after endotoxin stimulation is …

Gene Expression In Tumor And Adjacent Normal Tissues In Lung Adenocarcinoma Subtypes, Olga Y Gorlova, Ivan P Gorlov, R Taylor Ripley, Chao Cheng, Yafang Li, Bo Peng, Yanhong Liu, Hee-Jin Jang, Sung Wook Kang, Claire Lee, Priyanka Ranchod, Bryan M Burt, Hyun-Sung Lee, Christopher I Amos

Faculty, Staff and Students Publications

Background: Lung adenocarcinoma (LUAD) has several histologically distinct subtypes that differ by a number of clinical features including patient survival. Molecular mechanisms underlying histological and clinical differences between subtypes remain poorly understood.

Methods: We conducted a comparative analyses of gene expression in acinar, lepidic, papillary and solid subtypes, as well as mucinous adenocarcinoma. We used a novel, more efficient approach to identify subtype-specific genes. We compared the mean gene expression level separately for tumors and adjacent normal tissue with pure or a highly represented (≥ 75%) subtype of interest to the mean expression in tumors where the subtype of interest …

Spatial Proximity Of Immune Cell Pairs To Cancer Cells In The Tumor Microenvironment As Biomarkers For Patient Stratification, Jian-Rong Li, Xingxin Pan, Yupei Lin, Yanding Zhao, Yanhong Liu, Yong Li, Christopher I Amos, Chao Cheng

Faculty, Staff and Students Publications

Background/objectives: The tumor microenvironment (TME) plays a critical role in cancer progression by shaping immune responses and influencing patient outcomes. We hypothesized that the relative proximity of specific immune cell pairs to cancer cells within the TME could help predict their pro- or anti-tumor functions and reflect clinically relevant immune dynamics.

Methods: We analyzed imaging mass cytometry (IMC) data from lung adenocarcinoma (LUAD) and triple-negative breast cancer (TNBC) cohorts. For each immune cell pair, we calculated a relative distance (RD) score, which quantifies the spatial difference in proximity to cancer cells. We assessed the prognostic and predictive significance of these …

Ablation Of The Evolutionarily Acquired Functions Of The Atp1b4 Gene Increases Metabolic Capacity And Reduces Obesity, Nikolai N Modyanov, Lucia Russo, Sumona Ghosh Lester, Tamara R Castañeda, Himangi G Marathe, Larisa V Fedorova, Raymond E Bourey, Sonia M Najjar, Ivana L De La Serna

2020-Current year OA Pubs

In placental mammals, the co-option of vertebrate orthologous

Impactor Displacement As A Predictor Of Thoraco-Abdominal Organ Injury: Comparison Of Isolated Organ To Whole Body Tests, Jack Seifert, Jared Michael Koser, Alok Shah, Lance Frazer, Frank A. Pintar, Narayan Yoganandan, Dan Nicolella, Timothy B. Bentley, Brian D. Stemper

Biomedical Engineering Faculty Research and Publications

Purpose

Body armor is used to protect the wearer from penetrating injuries. However, when the armor defeats the projectile, it deforms at high rates into the wearer, referred to as back face deformation (BFD). This deformation can cause a variety of superficial and internal injuries and consequently should be considered when designing body armor. However, current design standards do not adequately consider the effects of BFD, and new data are necessary to inform future design thresholds for BFD. The objective of this study was to develop blunt impact injury risk curves for in vitro thoraco-abdominal organs and determine their applicability …

Prevalence And Risk Factors Of Concurrent Osteoarthritis And Urolithiasis In Dogs In Thailand (2018–2020), Faiz Ihsanul Kamil, Kumpanart Soontornvipart

The Thai Journal of Veterinary Medicine

Osteoarthritis is a degenerative joint disease that significantly impacts dog health, often leading to chronic pain, reduced mobility, and behavioral changes that may predispose dogs to secondary conditions such as urolithiasis. This study aimed to investigate the association between osteoarthritis and urolithiasis in dogs using radiographic imaging data collected from the Small Animal Teaching Hospital, Chulalongkorn University, Thailand, over a three-year period (January 2018 to December 2020). A total of 16,377 dogs were included, and cases of osteoarthritis, urolithiasis, or the concurrent occurrence of both conditions were identified through a systematic database review. Binary logistic regression models were used to …

Impact Of Primary Care Healthcare Providers Patient Electronic Medical Records Completion Rates Using Speech Recognition Dictation, Pepper Losely

Student Scholarly Projects

Practice Problem: Completion of electronic medical records (EMRs) is an ongoing problem for providers, facilities, and insurance reimbursement. Current completion of EMRs lack standardized processes to make EMRs completion easier for providers with their busy workday and allow optimal time for patient care.

PICOT: For healthcare providers at primary clinics (P) will implementing speech recognition dictation (SRD) for data entry into the electronic medical records (EMRs) (I) compared to the current practice of manually typing all data causing long medical record turnaround times (c) improve medical record completion within 30 days (O) over 10-weeks (T).

Evidence: Evidence that was …

Assessing Natural Resources Conservation Service (Nrcs) Impacts On Rice And Soybean Efficiency In The Mid-South, Ayodeji Samuel Ayiti

LSU Master's Theses

The study investigates how the Mid-South region of the U.S. rice and irrigated soybean production efficiency is influenced by (NRCS) incentives. To evaluate the effect of crop insurance, subsidies, and conservation incentives on lowering inefficiency, this study used a stochastic frontier model (SFA). The dataset covered the years 2010 – 2023, which allowed for a thorough evaluation of efficiency trends over time. While crop insurance programs reduce inefficiency in rice production, it shockingly increases inefficiency in soybean production, according to the study, which highlights the disparate effects of net support (insurance-subsidy).

The results show that while external factors like changes …

Digital Technology Usage In Tajikistan, Siyovush Bobokhonov Dr., Pedro Quelhas Brito Dr., Umesh Ramchandra Raut Dr., Prafulla Arjun Pawar Dr.

The Qualitative Report

The rapid development of digital technologies is significantly changing how people live, and these changes have a particular impact on the younger generation in Tajikistan. Our study therefore aims to analyze the factors influencing young Tajiks' opinions as users of digital technology and to examine digital technology usage in Tajikistan. We conducted a qualitative analysis of the opinions of young Tajik individuals aged 18 to 30 and found that they generally consider digital technology to be an integral part of their daily lives. Data collection was carried out through focus group discussions (FGDs) and the data analysis was performed using …

Mbiochemical Identification And Clinical Description Of Edetomidine Exposure In People Who Use Fentanyl In Philadelphia, Pa, Phil Durney, Jennifer L. Kahoud, Tareva Warrick-Stone, Maeve Montesi, Meg Carter, Sabrina Butt, Alberto Martinez Mencia, Louisa Omoregie, Monali Shah, Mariah Bloomfield, Nicholas Tomasko, Rebecca C. Jaffe, Allison Herens, Warren R. Korn, Karen Alexander, Douglas F. Stickle, Dennis Goodstein, Lara Weinstein, Kory S. London

Department of Emergency Medicine Faculty Papers

Medetomidine, a veterinary α2-adrenergic agonist, has recently emerged as an adulterant in the non-medical opioid supply, yet human exposure has remained poorly characterized. We conducted a pragmatic retrospective cohort analysis utilizing chart review and liquid chromatography-tandem mass spectrometry (LC-MS/MS) toxicology testing on available urine samples from patients presenting to two hospitals in Philadelphia, PA, who fit two clinical phenotypes, intoxication or withdrawal. Samples also underwent glucuronidase pre-treatment to assess impact on the yield of medetomidine and xylazine metabolite detection. Testing identified universal exposure to medetomidine (58/58 samples) via the 3-hydroxy-medetomidine (3-OH-M) metabolite, post glucuronidase treatment and variable xylazine exposure (40/58 …

Defining Economic And Environmental Typologies Across 77 Countries To Prioritize Opportunities For Nonsewered Sanitation, Hannah A. C. Lohman, Yalin Li, Xinyi Zhang, Victoria L. Morgan, Shion Watabe, Stetson Rowles, Roland D. Cusick, Jeremy S. Guest

Civil Engineering & Construction: Faculty Publications

Lack of access to sanitation is a challenge that persists globally, with low sewerage connection rates in many low- and lower-middle-income countries. Engineered nonsewered sanitation (NSS) technologies can meet treatment requirements without sewers, but their relative sustainability varies across potential deployment sites. Here, we characterize the costs and carbon intensity (CI) of three emerging NSS technologies, two community reinvented toilets (CRTs) and one Omni Processor (OP), across 77 countries, identify sustainability performance typologies, and map typology prevalence in countries across the globe. Locality-specific factors such as wages, diet, and material costs drive regional variability in NSS costs by up to …

From Life-Vests To Catalysts: Collaboration As The Key To Becoming Researchers, Ingunn Skjesol Ph.D., Krisitn B. Ørjasæter Ph.D.

The Qualitative Report

Ph.D. students report that doctoral work is challenging, with tight deadlines and extensive individual demands. In our duoethnography we reflect on and interrogate how collaborating in a structured way supported the development of our research competence during our doctoral work. Our analysis draws on a range of data: emails, Outlook calendars, work notes, manuscripts with reviewing comments, audio recordings of workshops, memories and research diaries. This article presents two concepts: (1) the phasing of doctoral work, showing our journey from life-vests to catalysts, and (2) a structured model for collaboration, built on shared engagement and mutual commitment. Further it argues …

Navigating Uncertainty In Doctoral Education, Megan K. Mitchell, Audra Skukauskaitė, Lakelyn E. Taylor, Kathryn Babb, Nicole Narkiewicz, Joseph E. Lloyd

The Qualitative Report

The shift to more virtual learning during and since the COVID-19 pandemic has disrupted in-person doctoral education by limiting opportunities for face-to-face academic socialization, networking, and collaborative learning, prompting questions about how graduate students could sustain their scholarly development through online spaces. This study examines how graduate students who joined the Qualitative Research Club, a virtual interdisciplinary student organization, co-constructed a shared academic culture, or languaculture, through their interactions. Using an Interactional Ethnographic approach, we analyzed video and audio recordings, meeting artifacts, and discourse from club meetings to trace members' participation, identity development, co-construction of knowledge, and development of …

Honoring Queer Womxn Of Color’S Wisdom, Healing And Joy, Sydney M. Herring-Alderete, Miranda E. Gutierrez Acosta, Sarah M. Bryant, Michelle A. Pollok, Grace Schroeder, Kevlyn M. Holmes, Jessi H. Pham, Lauren Bulalacao, Suzanne Lopez, Moises Franco, Nat Betancourt Arellano, Lauryn Jarvis, Ana G. Reyes

The Qualitative Report

Too frequently, traditional research centers the wounded-subject position (Brown, 1995), which reduces minoritized individuals to their experiences of discrimination, perpetuating deficit-based narratives, oppression, and existing power dynamics. To counter traditional research frameworks and deficit-based narratives, we used photovoice methodology (Wang & Burris, 1997) to honor and celebrate the healing experiences of nine queer womxn of color (QWoC). The photovoice project explored the healing experiences of QWoC inside and outside counseling and lead to the curation of a photovoice exhibit to share their stories of resilience and power. This article outlines our approach to organizing a photovoice exhibit and our experiences …

A Mouse Model Engineered To Spatiotemporally Control Cre Expression In Progesterone Receptor Positive Cells†, Elvis Quiroz, Ryan M Marquardt, Shu-Yun Li, Artiom Gruzdev, David Cunefare, Charan Ganta, San-Pin Wu, John P Lydon, Francesco J Demayo

Faculty, Staff and Students Publications

The Cre/loxP system is widely used for site-specific genetic manipulation in mice. The PgrCre mouse model, where Cre recombinase is driven by the progesterone receptor promoter, is commonly used for gene ablation in Pgr-positive uterine cells. However, the PgrCre is active in the neonatal uterus and does not allow temporal control. To enhance the functionality of the PgrCre mouse, we generated and characterized an inducible PgriCreERT2 mouse, in which iCreERT2 is inserted downstream of the endogenous Pgr promoter. PgriCreERT2 mice crossed with Rosa26-CAG-LSL-Sun1-sfGFP-myc reporter mice demonstrated tamoxifen-dependent recombination in uterine stromal fibroblasts and a subset of epithelial cells. Tamoxifen-induced ablation …

Ovochymase 2 Is A Key Regulatory Factor Modulating Proteolytic Pathways And Sperm Maturation In The Mammalian Epididymis, Katarzyna Kent, Kaori Nozawa, Antrix Jain, Anna Malovannaya, Thomas X Garcia, Martin M Matzuk

Faculty, Staff and Students Publications

Spermatozoa acquire fertilizing competence during epididymal transit through proteolytic, chaperone-mediated, and post-translational modifications. Ovochymase 2, an epididymis-specific trypsin-like serine protease, has emerged as a central regulator of this maturation process. Here, we integrate targeted gene disruption, comprehensive proteomic profiling, and affinity-based proteome enrichment to delineate how Ovochymase 2 influences sperm functionality. Deletion of Ovochymase 2 disrupts the proteome of epididymal sperm, resulting in diminished levels of core fertility-related factors-including a disintegrin and metalloprotease domain 3, β-defensins, and protease-inhibitor complexes-while inducing compensatory upregulation of alternate proteases and chaperones. Interaction assays confirm direct or indirect associations between Ovochymase 2 and sperm surface …