Digital Commons Network^™

Autoregulation Of Rpl7b By Inhibition Of A Structural Splicing Enhancer, David Granas, Ishan Gammadde Hewa, Michael A White, Gary D Stormo

2020-Current year OA Pubs

Yeast ribosomal protein gene RPL7B is autoregulated by inhibition of splicing. The first intron has a "zipper stem" that brings the 5' splice site near the branch point (BP) and serves as an enhancer of splicing that is required for efficient splicing because the intron has a nonconsensus BP sequence of UGCUAAC. The intron also contains an alternative, and mutually exclusive, structure that is conserved across many yeast species. That conserved structure is a binding site for the Rpl7 protein so that when the protein is in excess over what is required for ribosomes, the protein binds to the conserved …

Direct Histone Proteoform Profiling Of The Unannotated, Endangered Coral Acropora Cervicornis, Cassandra N Fuller, Sabrina Mansoor, Kevin Jeanne Dit Fouque, Lilian Valadares Tose, Javier Rodriguez-Casariego, Mariangela Kosmopoulou, Detlev Suckau, Francisca N De Luna Vitorino, Benjamin A Garcia, Jose M Eirin-Lopez, Francisco Fernandez-Lima

2020-Current year OA Pubs

Epigenetic modifications directly regulate the patterns of gene expression by altering DNA accessibility and chromatin structure. A knowledge gap is presented by the need to directly measure these modifications, especially for unannotated organisms with unknown primary histone sequences. In the present work, we developed and applied a novel workflow for identifying and annotating histone proteoforms directly from mass spectrometry-based measurements for the endangered Caribbean coral Acropora cervicornis. Combining high-accuracy de novo top-down and bottom-up analysis based on tandem liquid chromatography, trapped ion mobility spectrometry, non-ergodic electron-based fragmentation, and high-resolution mass spectrometry, near complete primary sequence (up to 99%) and over …

Unlocking Efficiency In Real-World Collaborative Studies: A Multi-Site International Study With One-Shot Lossless Glmm Algorithm, Jiayi Tong, Chongliang Luo, Et Al.

2020-Current year OA Pubs

The widespread adoption of real-world data has given rise to numerous healthcare-distributed research networks, but multi-site analyses still face administrative burdens and data privacy challenges. In response, we developed a Collaborative One-shot Lossless Algorithm for Generalized Linear Mixed Models (COLA-GLMM), the first-ever algorithm that achieves both lossless and one-shot properties. COLA-GLMM ensures accuracy against the gold standard of pooled data while requiring only summary statistics and completes within a single communication round, eliminating the usual back-and-forth overhead. We further introduced an enhanced version that employs homomorphic encryption to reduce the risks of summary statistics misuse at the coordinating center. The …

Chondrocyte-Specific Knockout Of Piezo1 And Piezo2 Protects Against Post-Traumatic Osteoarthritis Structural Damage And Pain In Mice, Erica V Ely, Kristin L Lenz, Sophie G Paradi, Seth Ack, Abraham Behrmann, Sarah Dunivan, Lauryn Braxton, Wolfgang Liedtke, Yong Chen, Kelsey H Collins, Farshid Guilak

2020-Current year OA Pubs

BACKGROUND: Osteoarthritis (OA) is a debilitating joint disease characterized by cartilage degeneration, synovial inflammation, and bone remodeling, with limited therapeutic options targeting the underlying pathophysiology. Mechanosensitive ion channels Piezo1 and Piezo2 play crucial roles in chondrocyte responses to mechanical stress, mediating mechanotransduction pathways that influence chondrocyte survival, matrix production, and inflammatory signaling, but their distinct contributions to OA pathogenesis remain unclear.

METHODS: Using inducible, chondrocyte-specific Aggrecan-Cre (Acan) mice, we investigated Piezo1, Piezo2, and combined Piezo1/2 conditional knockouts (cKOs) using the destabilization of the medial meniscus (DMM) model of post-traumatic OA in male and female mice. Pain and behavioral assessments were …

Stratifying The Population Based On Health Risk: Identification Of Patient Key Health Risk Factors Through Consensus Techniques, Carolina Castagna, Andrew Huff, Aaron Douglas, Phd, Matteo Garofano, Massimo Fabi, Richard W. Hass, Vittorio Maio

College of Population Health Faculty Papers

BACKGROUND: Risk stratification is a population health management approach that classifies patients according to their health risks and projected care needs. This strategy is especially valuable in primary care, where timely interventions for high-risk individuals can lead to better health outcomes, reduced healthcare expenditures, and a more sustainable healthcare system. The goal of this study was to establish expert consensus on the clinical and sociodemographic patient factors that should be incorporated into a primary care risk stratification tool.

METHODS: A multidisciplinary expert panel of 24 healthcare professionals, including primary care providers (PCPs), specialists, and allied health professionals, was convened in …

Incidence Of Heart Failure Following Carpal Tunnel Release Surgery, Harrison S. Fellheimer, Mahaa Ayub, Gabriel I. Onor, Lauren O'Mara, Eric Tecce, Pedro K. Beredjiklian

Department of Orthopaedic Surgery Faculty Papers

PURPOSE: Previous literature suggests a relationship between carpal tunnel syndrome (CTS) and heart failure (HF), indicating that patients with CTS are more likely to experience cardiomyopathy. Amyloid deposition leading to CTS may be prodromal for the development of amyloid cardiomyopathy. We hypothesized that patients undergoing surgery for CTS would have an increased risk of HF compared to those who did not undergo surgery.

METHODS: The TriNetX database was queried using the primary ICD-10 code for CTS to identify patients. Patients were included if they had surgical treatment for CTS, reported as endoscopic or open carpal tunnel release, or median nerve …

Pembrolizumab And Lenvatinib In The Treatment Of Recurrent Ovarian Carcinoma: A Single Institution Experience, Helen Toma, Rebeca Kelly, Chelsea Katz, Hannah Hong, Hannah Diasti, David P Warshal, Lauren Krill

Kimmel Cancer Center Faculty Papers

BACKGROUND: Advanced stage ovarian carcinoma has a poor prognosis with recurrence rates of over 80%, 5-year survival of 36-45%, and limited response to standard therapy. Pembrolizumab and lenvatinib are FDA approved for treatment of microsatellite stable (MSS)/mismatch repair proficient (pMMR) endometrial and renal cell cancers. Early phase II studies have shown promising results in a variety of advanced solid tumors, including ovarian cancer. We report on the clinical outcome of recurrent MSS/pMMR ovarian cancer patients treated with this therapy.

METHODS: For this retrospective cohort study, patients with a diagnosis of ovarian cancer treated with pembrolizumab and lenvatinib from January 2020 …

Rayion Smith, Rayion Smith '26, Nathalie Romero '25, Posse Legacy Project, Illinois Wesleyan University

All oral histories

Interview of Rayion Smith on July 19, 2025. Rayion Smith is a Posse 6 Scholar. Her major is in Biology with a minor in Public Health.

Liberalism And Lockdowns, D. Robert Macdougall

Publications and Research

Some authors have argued that liberalism cannot support lockdowns because it cannot engage in collective welfare-type reasoning. Others have thought that liberalism can justify lockdown measures, but that legitimate lockdown measures must be based on pre-legal moral principles that set acceptable limits to the imposition of risk or forbid contributing to collective harm. In this essay, I assess the overall relationship between liberalism and lockdowns. Against the first interpretation of liberalism, I argue in favor of the idea that lockdowns can be justified by liberalism. Liberalism protects the liberty of each and so can presumably protect persons from some kinds …

Family Caregivers’ Perceptions Of Quality Of Dementia Self-Care In Wakiso District, Uganda, Martha Sajatovic, Kylie Meyer

Faculty Scholarship

INTRODUCTION: In Sub-Saharan Africa, most people with dementia are cared for in the home setting (dementia self-care) with hardly any support from the formal health and social care system. The study explored family caregivers' perceptions of quality of dementia self-care. METHODS: A descriptive-exploratory qualitative study design was used. Data were collected through four focus group discussions with 48 family caregivers of older people with dementia, recruited from four villages of the Wakiso district in Uganda. RESULTS: Five themes emerged: (1) patience and understanding; (2) maintaining hygiene and cleanliness; (3) constant supervision and safety precautions; (4) personalized care by understanding individual …

Genes Encoding Multiple Modulators Of The Immune Response Are Methylated In The Prostate Tumor Microenvironment Of African Americans, Vinay Kumar, Tara Sinta Kartika Jennings, Lucas Ueta, James Nguyen, Liankun Song, Michael Mcclelland, Weiping Chu, Michael Lilly, Michael Ittmann, Patricia Castro, Arash Rezazadeh Kalebasty, Dan Mercola, Omid Yazdanpanah, Xiaolin Zi, Farah Rahmatpanah

Faculty, Staff and Students Publications

Background/Objectives: Prostate cancer (PCa) is diagnosed at an earlier median age, more advanced stage, and has worse clinical outcomes in African American (AA) men compared to European Americans (EA).

Methods: To investigate the role of aberrant DNA methylation in tumor-adjacent stroma (TAS), methyl binding domain sequencing (MBD-seq) was performed on AA (n = 17) and EA (n = 15) PCa patients. This was independently confirmed using the long interspersed nuclear element-1 (LINE-1) assay. Pathway analysis was performed on statistically significantly differentially methylated genes for AA and EA TAS. DNA methylation profiles of primary cultured AA and …

Mouse Metastable Epialleles Are Extremely Rare, Chathura J Gunasekara, Uditha Maduranga, Taylor Zhang, Jonathan N Wells, Maria S Baker, Eleonora Laritsky, Yumei Li, Cristian Coarfa, Yi Zhu, Robert A Waterland

Faculty, Staff and Students Publications

Metastable epialleles (MEs) are genomic loci at which epigenetic marks are established stochastically during early embryonic development and maintained during subsequent differentiation and throughout life, leading to stable epigenetic and phenotypic variation among genetically identical individuals. Although MEs were first described in mice over 20 years ago, the extent of epigenetic metastability in the mouse genome remains unknown. We present the first unbiased genome-wide screen for MEs in mice. Using deep whole-genome bisulfite sequencing across tissues derived from the three embryonic germ layers in isogenic C57BL/6J mice, we identified only 29 MEs, precisely localizing them and documenting their rarity. Consistent …

Smartboot: Real-Time Monitoring Of Patient Activity Via Remote Edge Computing Technologies, Gozde Cay, Myeounggon Lee, David G Armstrong, Bijan Najafi

Center on Aging Staff Publications

Diabetic foot ulcers (DFUs) are a serious complication of diabetes, associated with high recurrence and amputation rates. Adherence to offloading devices is critical for wound healing but remains inadequately monitored in real-world settings. This study evaluates the SmartBoot edge-computing system-a wearable, real-time remote monitoring solution integrating an inertial measurement unit (Sensoria Core) and smartwatch-for its validity in quantifying cadence and step count as digital biomarkers of frailty, and for detecting adherence. Twelve healthy adults wore two types of removable offloading boots (Össur and Foot Defender) during walking tasks at varied speeds; system outputs were validated against a gold-standard wearable and …

Targeted Delivery Of Rapamycin Via Epidermal Growth Factor Receptors In Pancreatic Cancer Cells Inhibits Cell Proliferation And Induces Apoptosis, Nawab Ali, Adeolu S. Oluremi, Christopher Baldwin, Nickolas Gustavison, Mujeebat Bashiru, Adeniyi Oyebade, Noureen Siraj, Raj Raghavendra Rao

Faculty Scholarship

Pancreatic cancer (PC) is aggressive and deadly; most PC cells overexpress an epidermal growth factor receptor (EGFR). EGFR-targeting peptide (GE11) was conjugated to gold nanoparticles (AuNPs) for delivering rapamycin (Rap), an mTOR inhibitor that reduces proliferation and induces apoptosis. Biophysical techniques confirmed the conjugation. Rap loading efficiency was 41.5%, with sustained release at a lower pH. Conjugates showed higher uptake in PANC-1 cells than unmodified AuNPs. It also inhibited proliferation and induced apoptosis via pathways involving reactive oxygen species, mitochondrial damage, and caspase activation. This approach represents a promising nanoparticle-based therapeutic strategy targeting PC cells, utilizing GE11-EGFR interactions to minimize …

Accuracy Of Anthropometric Equations In Estimating Body Fat Percentage In Transgender Men Undergoing Gender-Affirming Hormone Therapy, Barbara Buosi, Yana Barros Hara, Rodolfo André Dellagrana, Rafael Martins Da Costa, Mateus Rossato

International Journal of Exercise Science

International Journal of Exercise Science 18(1): 686-694, 2025. Transgender people may opt for the use of sex steroids and, in some cases, hormone blockers, which can lead to changes in body fat distribution. The aim of this study was to verify the accuracy of anthropometric equations to estimate the body density (BD) of transgender men who are undergoing Gender-Affirming Hormone Therapy (GAHT). Four transgender men (25-32 years old), who had performed GAHT for at least six months and used the hormone testosterone cypionate participated in the study. The reference criteria for BD was air displacement plethysmography (ADP). In addition, BD …

Understanding Socio-Environmental Concerns About Bear Lake: Findings From A Community-Engaged Teaching Study, Stacia Ryder, Alexander Theophilus, Charlotte Emelia Williams, Elizabeth Brunner, Jessica Ulrich-Schad, Mufti Nadimul Quamar Ahmed, Zubair Barkat, Nowrin Fatema, Jordan Rowley

Transforming Communities

In the western U.S., climate change is exacerbating already difficult situations in many lake systems, including Bear Lake, which spans the Idaho-Utah border and faces several environmental issues and a new wave of recreational development. In this research note, we discuss preliminary findings from community-engaged research aimed at assessing different concerns that scientists, residents, and community organizations have for Bear Lake and the surrounding communities. To do so, this project connects student research across three courses. Data collected through archival and interview research provides important insights into the socio-environmental concerns related to 1) ecological threats, 2) water management, quality, and …

Benefits And Impact Of Global And Bilingual Education: Dual Master’S Degree In Spanish Language And Culture For Educators, Diana Meza, Felix Wang, Yenisei Montes De Oca

International Journal on Responsibility

The dual master’s degree program in Spanish Language and Culture (SLC) for educators represents a pioneering initiative, emphasizing internationalization and global education through collaboration between James Madison University (JMU) and the University of Salamanca (USAL). This program addresses the escalating demand for bilingual education and the growing population of Spanish speakers in the educational landscape of the United States. The SLC program highlights educators’ responsibility to engage with and promote cultural and linguistic diversity by supporting bilingualism and cultural literacy in diverse communities where Spanish is widely used, particularly in regions where Spanish-speaking populations are marginalized. The program offers the …

Inter-Effort Recovery Intermittent Hypoxia And Force Parameters In Sprint Interval Exercise, Tarine Botta De Arruda, Felipe Alves Ribeiro Ms, Germano Marcolino Putti, Marcelo Papoti

International Journal of Exercise Science

International Journal of Exercise Science 18(3): 672-685, 2025. Performing training sessions in hypoxia leads to a decrease in effort quality. The inter-effort recovery intermittent hypoxia model seems to ensure training session performance. The aim of this study is to investigate the effects of sprint interval exercise under normoxic (NOR), continuous hypoxic (HYP), and inter-effort hypoxic (IEH) conditions on force parameters. Seven swimmers (age: 26.04 ± 4.64 years) volunteered and performed one session of the 10 × 30-s all-out tethered swimming efforts with 4-min passive recovery intervals for each condition. Considering the simulated altitude at F_iO₂ = 0.13 …

The Influence Of "Ifbb Pro" Designation On Consumer Decision-Making In Online Personal Training Services: An Experimental Investigation, Zehua Hei, Alan Morse

Global Sport Business Journal

The recent rising health awareness, social media occurrence, and digital access to fitness services promote the growth of online personal training. Drawing upon signaling theory, a recent rising group of trainers, “IFBB Pro” athletes, who are perceived as elite bodybuilders, often signal a high level of expertise and competency in fitness training. With content marketing as the background, posted content plays a key role in this process as social media platforms become increasingly popular for fitness professionals to market their personal training services and attract clients. However, scarce research exists to investigate how this designation influences consumer perceptions and purchase …

Straddling The Boundary Of Misleading Information Group: Exploring The Association Between Network Brokerage And Morality Expressions, Yiqi Li, Marina Mitiaeva, Ximeng Deng, Una Joh, Yafei Zhang

iSchool - All Scholarship

Existing misleading information studies mostly focus on either the message content or social network structures, overlooking the association between the content and structural factors. This research examines the role of brokers, the key network actors straddling between misleading and true/regular information groups. Content-wise, morality expressions are examined, which signal social media users’ expressions toward what is right and wrong. This research provides insights into the understudied relationship between network brokerage and moral expressions and identifies connections between moral foundation and network brokerage theories. Findings suggest that binding morality (i.e., authority and loyalty) is dominant among network brokers. Practical strategies are …

Development And Validation Of The Informal Safety Leadership Measure, Ashley L. Gill

Dissertations

This research develops and validates a measure of the emerging concept of informal safety leadership (ISL), which integrates elements of safety leadership and informal leadership to address a significant gap in organizational safety literature. Despite extensive research on formal safety leadership, there remains limited understanding of how non-managerial employees influence safety practices and culture within organizations. This dissertation develops and validates a comprehensive measure of informal safety leadership through a sequential mixed-methods approach incorporating a pilot study and two additional studies. First, a pilot study employed focus groups with health, safety, and environmental professionals to identify key dimensions of ISL. …

A Cancer Education Needs Assessment: Informing Middle-Aged Female Patients About The Relationships Between Obesity And Women’S Health Concerns In The Reproductive System, Breast, And Endometrial Health, Batul Mirza

MUSC Theses and Dissertations

Obesity significantly impacts women’s health, particularly among middle-aged women, by increasing the risk of hormone-sensitive cancers such as breast, endometrial, and reproductive system cancers. This study examines the educational needs of this demographic group regarding obesity-related cancer risks and explores effective intervention strategies. Obesity-induced mechanisms – hormonal imbalances, chronic inflammation, and insulin resistance – drive cancer susceptibility, emphasizing the need for targeted health education. The study employs a qualitative design, which includes interviews with subject matter experts (SMEs) and surveys of middle-aged women. The goal is to assess awareness, perceived barriers, and preferred learning methods. Findings suggest that with many …

The Physical Characteristics Linked To The Perception Of Criminality Of Female Offenders, Jennifer Anne Chapman

Walden Dissertations and Doctoral Studies

Gender disparities can be seen throughout the criminal justice system, where females are seen as weak and incapable of causing harm. This quantitative study sought to add to the existing research regarding physical characteristics linked to criminality. The labeling theory explains how physical characteristics labeled as deviant can alter a person’s perception when deciding whether an individual is violent. The research questions explored the physical characteristics linked to the perception of violent female offenders as well as the relationship between the gender of the participant and their ability to successfully identify a violent female offender. A mixture of statistical designs …

Growing Healthy Minds: Ole Miss Conference Plants Seeds Of Wellness, Marisa C. Atkinson

University of Mississippi News

OXFORD, Miss. – Early childhood educators from across the state spent time learning how to positively and mindfully influence the lives of students and themselves this week at the University of Mississippi's inaugural Whole Child Wellness in Early Childhood Education Conference.

Designing Context-Specific Physical Activity Interventions For English Primary Schools: Key Learning From A Four-Month Rapid Ethnography, Robert Walker, Danielle House, Simona Kent-Saisch, Alice Porter, Ruth Salway, Lydia Emm-Collison, Michael W. Beets Med, Mph, Phd, David Revalds Lubans, Frank De Vocht, Russell Jago

Faculty Publications

Background

Physical activity is essential for children’s health. Primary schools offer an opportunity to equitably promote physical activity. However, school-based interventions have been shown to have little to no effect, potentially due to a lack of consideration of school heterogeneity. This study reports on a rapid ethnography study that was used to capture insights into English primary schools physical activity. The data are intended to inform the design of a context-specific intervention to improve pupil physical activity.

Methods

Three researchers conducted a four-month rapid ethnography study within three primary schools in Bristol, UK, between March and July 2024. Several methods …

Designing Context-Specific Physical Activity Interventions For English Primary Schools: Key Learning From A Four-Month Rapid Ethnography, Robert Walker, Danielle House, Simona Kent-Saisch, Alice Porter, Ruth Salway, Lydia Emm-Collison, Michael Beets Med, Mph, Phd, David Revalds Lubans, Frank De Vocht, Russell Jago

Faculty Publications

Background

Physical activity is essential for children’s health. Primary schools offer an opportunity to equitably promote physical activity. However, school-based interventions have been shown to have little to no effect, potentially due to a lack of consideration of school heterogeneity. This study reports on a rapid ethnography study that was used to capture insights into English primary schools physical activity. The data are intended to inform the design of a context-specific intervention to improve pupil physical activity.

Methods

Three researchers conducted a four-month rapid ethnography study within three primary schools in Bristol, UK, between March and July 2024. Several methods …

July 18, 2025 Ereporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

Life Cycle Assessment Of New Jersey Offshore Wind, Meghann Smith, Nawal Shoaib, Pankaj Lal

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

As offshore wind gains momentum within US (United States) renewable energy goals, New Jersey's ambitious targets for offshore wind development represent a significant opportunity to reduce emissions and transition towards cleaner energy sources. This study presents a life cycle assessment (LCA) of a planned offshore wind farm off of New Jersey's coast, emphasizing the implications of a domestic supply chain. Key findings suggest that the offshore wind farm is projected to produce 0.013 kg CO2 kWh−1 of electricity generated, reflecting a 98 % decrease in carbon emissions compared to natural-gas-derived electricity. Further, when compared to carbon emissions from other renewable …

Comparing And Evaluating Contralateral Breast Dose Relative To Median Ptv Tumor Bed Dose For Hypo-Fractionated Breast Treatments Using Import High Dose Constraints., Shannon J. Nordby

Masters Projects

This study is aimed to compare two different planning constraints for post-lumpectomy whole breast radiation treatments to evaluate whether or not the mean dose to the contralateral breast can be significantly reduced while maintaining plan quality for treatment. A total of 10 patients who had first undergone a lumpectomy were included into this study. Each patient’s initial treatment plan was created using a conventional approach with a mean dose constraint of < 600 cGy (Plan A) and then was replanned using the Import High protocol of a mean dose constraint, aiming to further push for a mean dose < 150 cGy (Plan B) to the contralateral breast. Both of these plans were analyzed for PTV coverage and dose to the surrounding critical structures. The results have shown that the contralateral mean dose was significantly reduced from an average of 216.32 in Plan A to 126.53 cGy in Plan B. All while meeting the target coverage of > 95% isodose line. Statistical testing showed a significant difference between the two planning techniques of lowering the contralateral dose (p < 0.0001), and normality testing supported the validity of the data analysis. In addition to a substantial dose reduction to the contralateral breast, Plan B maintained equal or improved dose constraint to other surrounding critical organs at risk, including the heart, lungs, spinal cord, and thyroid. The Import High protocol demonstrated consistent outcomes across diverse patient anatomies. These findings suggest that by using the Import High mean dose constraint, it is possible to maintain plan quality while reducing dose to the contralateral breast. This approach may help reduce the risk of long-term side effects and improve the patient’s overall outcomes in whole breast radiation treatments.

The Silent Identity: Asexuality, Aromanticism, And Convention In Le Roman De Silence And The Story Of Silence, Anita Farrell

English and Creative Writing Summer Fellows

Le Roman de Silence is a 13th-century French romance about Silence, a woman raised as a man to protect their family’s inheritance. Heldris of Cornwall, the author, develops a rich inner world to help the audience connect with Silence, allowing them to experiment with gendered, sexual, and romantic boundaries. This romance intrigues modern scholars, especially feminist and queer researchers due to narrative pressure on conventions like gender and sex. In 2020, Alex Myers, a transgender author, updates Silence’s tale in The Story of Silence, where Silence explores their identity in greater detail. Silence eventually identifies themselves as non-binary and …