Digital Commons Network^™

The Microrna Mir-30a Blocks Adipose Tissue Fibrosis Accumulation In Obesity, Pradip K Saha, Robert Sharp, Aaron R. Cox, Rabie Habib, Michael J. Bolt, Jessica B. Felix, Claudia E. Ramirez Bustamante, Xin Li, Sung Yun Jung, Kang Ho Kim, Kai Sun, Huaizhu Wu, Samuel Klein, Sean M. Hartig

2020-Current year OA Pubs

White adipose tissue (WAT) fibrosis occurring in obesity contributes to the inflammatory and metabolic comorbidities of insulin resistance and type 2 diabetes, yet the mechanisms involved remain poorly understood. Here, we report a role for the broadly conserved miRNA miR-30a as a regulator of WAT fibrosis and systemic glucose metabolism. Mice modified to express miR-30a at elevated levels in adipose tissues maintain insulin sensitivity coupled with reduced fatty liver disease when fed a high-fat diet. These effects were attributable to cell-autonomous functions of miR-30a that potently increase expression of adipocyte-specific genes. Proteomic screening revealed miR-30a limits profibrotic programs in subcutaneous …

Impact Of Hereditary Angioedema Attacks On Health-Related Quality Of Life And Work Productivity, Maeve O'Connor, Paula J Busse, Timothy J Craig, Cristine Radojicic, H James Wedner, Sherry Danese, Julie Ulloa, Vibha Desai, Tomas Andriotti, Paul K Audhya, Sandra Christiansen

2020-Current year OA Pubs

BACKGROUND: Hereditary angioedema (HAE) negatively impacts health-related quality of life (HRQoL). Few studies have characterized impairments to HRQoL, work productivity, and well-being during and following HAE attacks.

METHODS: Patients with ≥1 HAE attack in the previous 3 months were recruited by the United States (US) HAE Association (HAEA) to complete an online survey. Respondents were categorized into those who did (Treated Cohort) and did not (Untreated Cohort) manage their last attack with on-demand treatment (OD). Adapted versions of the EuroQol-Five Dimensions-Five Levels (EQ-5D-5L), EuroQol Visual Analogue Scale (EQ VAS), and Work Productivity and Activity Impairment-General Health assessments were used.

RESULTS: …

Revised Draft Final Butte Treatment Lagoons (Btl) Groundwater Treatment System Routine Operations, Maintenance, And Monitoring (Om&M) Plan, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Final Quarterly Operations And Maintenance Report Butte Treatment Lagoon System – Fourth Quarter 2023, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Final Quarterly Operations And Maintenance Report Butte Treatment Lagoon System – Third Quarter 2023, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Revised Draft Final Quarterly Operations And Maintenance Report Butte Treatment Lagoon System – First Quarter 2024, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

The Silent Grief Of Grandmothers After An Out-Of-Order Death—An Interpretative Phenomenological Analysis, Jordan Robertson, Elizabeth Cutrer, Paul Caldarella, Jeremy B. Yorgason, Terrell Young, Erjola Gjini, Sarah Stuart, Savannah Tueller

Faculty Publications

This study delves into the lived experiences of grandmothers grappling with grief following the “out-of-order” death of a child, child-in-law, or grandchild, using an Interpretative Phenomenological Analysis grounded in the Dual Process Model-Revised. With 70% of U.S. adults over 65 being grandparents, the research highlights their often-invisible role as both grievers and caregivers amid family loss. Findings reveal three key themes: navigating personal grief, intergenerational support dynamics, and reconstructing family identity. Grandmothers oscillate between loss-oriented and restoration-oriented coping, with grief intensity varying by relational proximity—most profound when losing their own child. They provide emotional and practical support to surviving grandchildren, …

Improved Autofluorescence Lifetime Decay Analysis For Identification Of Cellular Autofluorescence Biomarkers For Label-Free, Single-Cell Assessment Of Mitochondrial Membrane Potential, Olivia Isabel Kolenc

Graduate Theses and Dissertations

Optical imaging of metabolism using the autofluorescence of the metabolic cofactors NADH, NADPH, and FAD has a wide range of sensitivities and the capability to collect a great deal of metabolic information. Multiphoton microscopy (MPM) is a non-invasive, label-free optical imaging technique capable of performing fluorescence lifetime imaging (FLIM) using time correlated single photon counting (TCSPC). However, autofluorescence FLIM presents a challenge in collecting sufficient photons to provide adequate signal to noise ratios (SNR) for analysis. Additionally, the specificity of autofluorescence biomarkers of metabolism has not been well-defined. Contextualizing these autofluorescence measures using established functional biomarkers can improve interpretability of …

Synthesis, Structural Characterization And Optical Studies Of Silver-Indium-(Zinc)-Chalcogenide Fluorescent Quantum Dots, Sujal Acharya

Graduate Theses and Dissertations

Developing a non-toxic, high-performance fluorescent nanomaterial is crucial for overcoming the environmental and health restrictions of current cadmium, and lead based quantum dots (QDs), which limit the application of quantum dots in optoelectronics and bioimaging. In this thesis, we synthesized environmentally friendly AgInS2 QDs by a colloidal method, systematically altering the In/Ag precursor ratio from 2 to 6 to study the impact on their optical and photophysical properties. Our goals were to find the optimal stoichiometry for maximum quantum efficiency and stability. We also investigated further improving optical and photophysical properties through shelling with ZnS. The emission spectra appeared broad, …

The Impact Of Mindfulness Therapy To Reduce Disruptive Behavior In High School, Wendy Nell Peters

Electronic Theses and Dissertations

The aim of this project was to determine the impact and effectiveness of Gina Biegel’s mindfulness techniques for stressed teens method on adolescent anxiety and the disruptive symptoms that lead to high school adolescents being assigned to detention. This project described a quantitative correlational design research project that investigated a small adolescent sample size, with a goal of decreasing the number of local high school adolescent students who are repeat detention offenders, by implementing Gina Biegel’s mindfulness techniques for stressed teens method on those high school adolescents who were assigned to detention. This was achieved by collecting demographic data from …

An Exploratory Descriptive Study Of Urban Professional High School Counselors' Work Culture, Burnout, And Mental Well-Being, Alexandria L. Hall

Electronic Theses and Dissertations

This descriptive, exploratory, qualitative study examined urban, public U.S. high school counselors’ perceptions of job-related stress, autonomy, and administrative support within urban school environments. Seven current high school counselors working in urban public schools were recruited through convenience sampling in educator forums on Facebook, followed by snowball sampling to expand the participant pool. Counselors participated in semistructured interviews conducted via Zoom or asynchronous online focus groups hosted on Padlet, depending on the participant’s preference. Data were analyzed using Braun and Clarke’s six-step thematic analysis process, supported by Dedoose software for coding and data management. The analysis revealed four major themes: …

Overcoming Second-Order Barriers To Teachers’ Technology Integration: A Study Of Positive Impacts, Crystal Ann Hume

Electronic Theses and Dissertations

This study explored K–12 public school teachers’ self-efficacy, value, and pedagogical beliefs about technology. The teachers worked in a medium-sized, semi-affluent public school district in North Texas. The study investigated second-order barriers to technology integration and what might positively impact teachers’ self-efficacy, value, and pedagogical beliefs. This study used a qualitative descriptive methodology to collect qualitative data about their beliefs by interviewing and observing K–12 classroom teachers who had completed a bachelor’s degree through a traditional teacher education program. Teachers with alternative certifications or noncertified classroom instructors were omitted. Findings revealed that external factors, failure, mistakes, confidence, and professional development …

Synthesis And Characterization Of Polymer-Coated Metal Nanostructures And Gelatin-Incorporated Hydrogels For Biomedical Use, Deborah Takyibea Okyere

Graduate Theses and Dissertations

The development of advanced materials for disease management and treatment presents a pressing challenge in the health sector. The overall objective of this research is to contribute to the development of advanced materials with tailored properties for biomedical applications, with a focus on cost-effective and robust nanoparticles and biomaterials. The first part of this work focuses on designing and synthesizing earth-abundant copper nanoparticles with desired optical properties. Copper nanoparticles, which provide a more affordable alternative to gold and silver, are being investigated due to their unique plasmonic properties. However, their susceptibility to oxidation limits their application. To address this challenge, …

Predicting Case Clearance For Victims Of Anti-Transgender Homicide In The United States, Brittaney Alyssa Mann

Graduate Theses and Dissertations

There is a shortage of research exploring case clearance of anti-transgender bias homicides. This study will comparatively examine the similarities and differences in anti-transgender bias homicide cases with and without arrests at the victim, situational and incident levels occurring in the United States between 2013 and 2023. Data are extracted from the Bias Homicide Database (BHDB), the National Coalition of Anti-Violence Programs (NCAVP), and the Human Rights Campaign (HRC). This project will address the gap in understanding case clearance of anti-transgender bias homicides, provide policy implications, and ideas to further the discipline’s understanding of the topic. Keywords: LGBTQIA+ victims, bias …

The Upcoming Moral Crisis In Primitive Artificial Intelligence, Justin E. Edens

Graduate Theses and Dissertations

As we continue to develop artificially intelligent systems, there is an increasingly high chance that we will develop a system that is both conscious and capable of suffering. Furthermore, it is likely that the development of this conscious machine will be entirely unintentional. While this machine will have moral status, identifying it will be extremely difficult, leading to it being treated the same as its inert predecessors. For these reasons I believe that a crisis in ethics is looming. This paper aims to argue that it is possible for a machine to have moral status, that the first such machine …

Pricing The Unknown: An Analysis Of Expected Value Of Young Horses And Price-Determining Factors, Rupasri Sura

Graduate Theses and Dissertations

From a seller’s point of view in a market, it is crucial to plan the pricing strategies based on perceptions of buyer preferences and their willingness to purchase to ensure market success in selling and pricing. Applying this to the equine industry, this paper studies the factors determining the price of young horses (age ≤ 2 years) in digital auction platforms. Using data from Professional Horse Services, LLC (2012–2023), a Heckman’s two-step approach was employed, which involves a probit model as the first step and a hedonic price model as the second, aiming to correct for sample selection bias in …

Using Interpersonal Motives To Identify Latent Profiles Of Borderline Personality Pathology: Implications For An Overcontrolled Style Of Bpd, Regina Schreiber

Graduate Theses and Dissertations

Borderline personality disorder (BPD) is conceptualized as a disorder of undercontrol (i.e., behaviorally impulsive, emotionally expressive), with affective, interpersonal, and identity instability. BPD related impairment is high, yet diagnostic accuracy remains a problem for individuals who present with greater overcontrol (i.e., behavioral inhibition, expressive suppression), which occurs in some BPD subtypes. The current study used ecological momentary assessment (EMA) to expand on prior research by identifying latent profiles based on interpersonal motives for agency and relatedness, desire for predictability, perceived dependency, and self-efficacy in a sample of people with elevated BPD features (n = 136). Individual differences in overcontrol, undercontrol, …

Resilience Redefined: Exploring The Impact Of Childhood Trauma On The Value Systems Of High-Achieving Corporate Men, Lana B. Henry

Graduate Theses and Dissertations

This qualitative study employed Interpretive Phenomenological Analysis (IPA) to explore how childhood trauma influences the value systems of high-achieving corporate men. In contemporary corporate culture, where stoicism, performance, and independence are often rewarded, little attention is paid to how early adversity informs internal frameworks of meaning, leadership, and success. Semi-structured interviews were conducted with six high-achieving men, all executives and leaders in high-performing industries, who self-identified as having experienced childhood trauma. Data were analyzed using a six-step IPA process and interpreted through the lens of Schwartz’s Theory of Basic Human Values and social constructivism. Findings revealed that childhood trauma deeply …

Examining The Use Of Two-Dimensional Video Capture And A Pressure Sensing Walkway For Collection Of Obstacle Crossing Measures, Ashlyn M. Jendro

Graduate Theses and Dissertations

Obstacle crossing research is crucial for providing insights into how and why people trip and fall. However, a growing body of literature has proposed that laboratory-based studies may not directly translate into real-world settings due to the complex nature beyond the sterile lab. Unfortunately, the absence of real-world obstacle crossing literature is likely due to a lack of available validated methods for collection of data outside the laboratory. This dissertation explored two potential methodologies (i.e., 2D motion capture and a pressure sensing walkway) that are more portable and cost-effective for collecting real-world obstacle crossing measures. It is hypothesized that 2D …

A Grounded Theory Investigation Of The Experiences Of Rehabilitation Administrators, Counselors, And Supervisors Providing Services To Women With Disabilities Experiencing Intimate Partner Violence, Dana Posey

Graduate Theses and Dissertations

Abstract There is a noticeable gap in the literature as it relates to how rehabilitation and disability service providers have worked with women with disabilities who have experienced intimate partner violence. To address the dearth of knowledge, a qualitative grounded theory study was conducted with 12 rehabilitation administrators, counselors, and supervisors regarding their experiences providing services to women with disabilities who have been in or are in an intimate partner violence relationship. Semi-structured interviews were administered, recorded, and transcribed verbatim in an ongoing manner throughout the process. As data was collected, it was analyzed using NVIVO 15, which is a …

Effects Of Multi-Sensory Training In Physical Activity On Reaction Time And Fitness In Elementary Students., Sarah Anne Trout

Graduate Theses and Dissertations

Abstract This study examined the effects of multi-sensory Neuroplasticity training (MSNP™) using the ROXPro system on the psychomotor abilities of third-grade students in a Midwestern elementary school. The research aimed to determine whether the integration of interactive sensory-reactive technology in physical education could improve reaction time, agility, and explosive power compared to standard PE instruction. A total of 49 participants were assigned to either a control group or an intervention group, with the latter engaging in six weeks of ROX-based drills. A pretest-posttest design was employed, and data were analyzed using ANCOVA, repeated measures ANOVA, and independent t-tests to assess …

Effect Of Marination And Storage Time On The Properties And Meat Quality Of Broiler Breast, Mayra Mabel Herrera Rivera

Graduate Theses and Dissertations

Abstract The poultry industry has implemented numerous methods, as marination and aging, to improve meat quality and satisfy consumer demands. Three experiments were developed to assess the effects of vacuum tumble marination and aging on the proteins of chicken breast. These experiments utilized 0.75 % sodium chloride, 0.45 % phosphate, and water in a marinade. In experiment one, 270 fillets were allocated into six treatment groups, each comprising 45 fillets. Six days of storage time were used, marinating and storing the fillets in each group at a temperature of 4°C. Following each day of storage period, the fillets underwent a …

Origin Of Purpose, Career Satisfaction, And Life Satisfaction: A Quantitative Study On Early Career Professionals, Anne K. Hocking

Electronic Theses and Dissertations

Since the early 2000s, research has increased on purpose and how it correlates with fulfillment outcomes in life and career satisfaction. This research has been valuable in professional and academic fields and helped develop leadership practices and interventions for organizations to increase satisfaction levels among employees and students. This study focused on the general problem that research dedicated to purpose has not adequately addressed whether origin of purpose affect its outcomes on fulfillment levels, particularly those of life and career satisfaction. To address this consideration, the researcher conducted a quantitative associative correlation to measure if and to what extent origin …

Changing The Perceived Pain Intensity In Populations With Spinal Cord Injury And With Health Disparities (Happiness): Protocol For A Feasibility Study, Ann Van De Winckel, Lin Zhang, Leslie R. Morse, Brianna Woodliff, Gladys Maestre, Kelsey Baker

School of Medicine Publications

Background: Chronic neuropathic pain affects 69% of adults with spinal cord injury (SCI). Medication, as a primary treatment, has insufficient benefits and significant risks for addiction and adverse effects. Of the available mind and body approaches, Qigong is the most accessible for adults with SCI, with evidence for effectiveness in reducing pain. Still, there is insufficient evidence to make recommendations for adults with SCI. Thus, the feasibility of Qigong in SCI needs to be established. We previously demonstrated feasibility in a pilot study, in which 18 non-Hispanic White adults with SCI completed a 12-week remote Qigong program. However, three additional …

Genetic Study Of Von Willebrand Factor Antigen Levels ≤ 50 Iu/Dl Identifies Variants Associated With Increased Risk Of Vwd And Bleeding, Rachel K. Friedman, Adam S. Heath, Jennifer E. Huffman, James T. Baker, Natalie R. Hasbani, Sarah A. Gagliano Taliun, Ming-Huei Chen, Tom Howard, Joanne E. Curran, John Blangero

School of Medicine Publications

Background: von Willebrand disease (VWD) is a common inherited bleeding disorder caused by low levels or activity of circulating von Willebrand factor (VWF). Genetic susceptibility to VWF antigen (VWF:Ag) below normal (≤50 IU/dL) in the general population is underexplored.

Methods: To identify genetic variants influencing VWF:Ag levels ≤50 IU/dL, we performed a genome-wide association study in 926 cases with VWF:Ag levels ≤50 IU/dL and 12,846 controls from 7 studies from the TOPMed program. We then examined whether genome-wide significant findings were also associated with clinical diagnosis of VWD in 5 biobanks with 708 VWD cases and 1,286,069 controls, and with …

Ambulatory Blood Pressure Monitoring, European Guideline Targets, And Cardiovascular Outcomes: An Individual Patient Data Meta-Analysis, Dong-Yan Zhang, De-Wei An, Yu-Ling Yu, Jesus D. Melgarejo, José Boggia, Dries S. Martens, Tine W. Hansen, Kei Asayama, Takayoshi Ohkubo, Gladys E. Maestre

School of Medicine Publications

Background and aims: Hypertension is the predominant modifiable cardiovascular risk factor. This cohort study assessed the association of risk with the percentage of time that the ambulatory blood pressure (ABP) is within the target range (PTTR) proposed by the 2024 European Society of Cardiology (ESC) guidelines for blood pressure (BP) management.

Methods: In a person-level meta-analysis of 14 230 individuals enrolled in 14 population cohorts, systolic and diastolic ABPs were combined to assess 24-h, daytime, and nighttime PTTR with thresholds for non-elevated ABP set at < 115/65, < 120/70, and < 110/60 mmHg, respectively.

Results: Median 24-h PTTR was 18% (interquartile range 5-33) corresponding to 4.3 h (1.2-7.9). Over …

Opportunistic Short-Term Water Uptake Dynamics By Subalpine Trees Observed Via In Situ Water Isotope Measurements, Matthias Sprenger, Stefan Seeger, Max Berkelhammer, Nathaniel A. Bogie, Raymond J. Hess, Wendy S. Brown, Sylvain Kuppel, James Knighton

Faculty Research, Scholarly, and Creative Activity

Variations in tree water sources are important to understand in semi-arid ecosystems because climatic shifts towards lower snowpack and increased drought affect water availability in subalpine forests of the western US. Here, we use daily in situ measurements of stable isotopes (²H & ¹⁸O) in soil and tree stem water, soil matric potential and sap flow to study tree water uptake dynamics. We instrumented three soil profiles down to 90 cm, as well as three aspen and engelmann spruce trees near Gothic, Colorado, in the East River watershed. We observed the fate of natural isotopic variations in …

Beyond Peak Water Security: Household-Scale Experiential Metrics Can Offer New Perspectives On Contemporary Water Challenges In The United States, Wendy Jepson, Amber Wutich, Amber L. Pearson, Melissa Beresford, Alexandra Brewis, Alicia Cooperman, Jeremiah Osborne-Gowey, Jenny Rempel, Asher Y. Rosinger, Justin Stoler

Faculty Research, Scholarly, and Creative Activity

The U.S. has moved beyond peak water security. Infrastructural degradation, institutional inertia, and climate change are reducing the ability of households and communities to benefit from near-universal safe, adequate, affordable, sustainable water services. Yet, current supply-side research tools, that focus largely on system performance, are not equipped to measure the prevalence and lived experiences of household water insecurity, thus limiting the evidence available to policymakers, utilities, and communities to make decisions about water services. We discuss how demand-side metrics, such as household-level water insecurity scales validated for high-income contexts, such as the U.S., can help stakeholders to better identify local …

Anticolonialism And Qualitative Methods For Culture-Centered Interventions, Mohan Jyoti Dutta, Ambar Basu, Satveer Kaur-Gill, Debalina Dutta, Mahuya Pal, Iccha Basnyat, Selina Metuamate, Venessa Pokaia, Phoebe Elers, Indranil Mandal, Rabindranath Mandi, Pankaj Baskey, Devalina Mookerjee, Shaunak Sastry, Jaime Robb, Andrew Carter

Faculty Research, Scholarly, and Creative Activity

In this essay, we a collective of Indigenous, Black, and migrant Global South scholars engaged in experiments with the culture-centered approach (CCA) draw on our lived experiences amidst struggles against land grab, neoliberal extractivism, and capitalist exploitation to outline a framework for qualitative methods as anticolonial politics. We begin by exploring the interplays of colonialism, imperialism, and racial capitalism that have shaped the origins and uses of qualitative methods toward serving extractive agendas of global capital. This critique serves as the basis for outlining the key principles of the CCA, turning to voice, storytelling, and embodied action as the basis …

A Protocol For The Development Of A Validated Scale Of Household Water Insecurity In The United States: Hwise-Usa, Amber L. Pearson, Wendy Jepson, Alexandra Brewis, Jeremiah Osborne-Gowey, Amber Wutich, Melissa Beresford, Asher Y. Rosinger, Adam M. Enders, Justin Stoler, Ellis Adjei Adams, Linda Mendez Barrientos, Laura A. Bray, Alida Cantor, Jennifer S. Carrera, Alicia Cooperman, Laura Castro Diaz, Valerisa Magnon Joe Gaddy, Leila M. Harris

Faculty Research, Scholarly, and Creative Activity

Background New metrics of household water insecurity have been validated for low- to middle-income countries, but it is unclear how these measurements apply to the experiences of people living in high-income countries. This project aims to develop and validate a novel metric for household water insecurity experiences in the United States (HWISE-USA) using a cross-sectional design and data from the Southwest, Midwest, and Western regions. Methods We outline the protocol for the development and validation of a novel household water insecurity scale for the United States to address this scientific need, including the following key steps: (1) item development through …