Digital Commons Network^™

Food Neophobia And Its Prevalence Among College Students In The United States, Joanne Mwendi Ponnappa

Master's Theses

Food neophobia “the reluctance to try unfamiliar foods” may influence diet variety and cultural food acceptance among young adults. College students represent a critical group for studying this behavior, as they are developing independent eating habits that can persist into adulthood. This study examined the prevalence of food neophobia among U.S. college students and evaluated how academic background, travel, cultural exposure, and demographic factors influence openness to trying unfamiliar foods. A cross-sectional online survey was administered via Qualtrics to 264 college students across the United States. The Food Neophobia Scale (FNS; Pliner & Hobden, 1992) was used to assess reluctance …

Rethinking Schedule Quality Risk Assessment, Michael E. Oliver, Patrick T. Rodjom

Beyond the Project Horizon: Journal of the Center for Project Management Innovation

Critical Path Method schedule reliability is dependent on exhibition of specific qualities. Failure to exhibit these qualities (i.e., schedule defects) increases project risk exposure. Although current schedule quality assessment methods provide diagnostic remediation insights, they do not provide a meaningful singular assessment value that characterizes the nature and magnitude of schedule quality risk nor do they enable parity when comparing schedule quality across multiple schedules of disparate size (e.g., number of tasks) or complexity (e.g., number of concurrent paths, degree of merge bias). This study employed a design science research approach to address current schedule quality assessment practice dilemmas through …

Community Banking, Tech Innovation Anchor Ole Miss Symposium, Marvis Herring

University of Mississippi News

OXFORD, Miss. – Community banks are critical to the nation's economic health, but they face unprecedented challenges from evolving technology, changing customer needs and economic headwinds, industry experts told participants at the University of Mississippi's 2025 Banking and Finance Symposium.

Advances In The Discovery Of Active Natural Products By Large Fragment Cloning, Dandan Zeng, Jiayu Chen, Tianlu Mo

Journal of Food Science and Biotechnology

Active natural products have diverse structures and activities and can be a major source of small molecule drugs.Given the emergence of multi-drug resistant bacteria and superbacteria,there is an urgent need to develop new active natural products.Large fragment cloning has been an important tool for the discovery of active natural products in recent years,and it has demonstrated obvious advantages in obtaining the biosynthetic gene clusters of active natural products.This approach,combined with efficient cluster-targeted screening methods,has significantly accelerated the discovery of active natural products.This review summarizes a wide variety of active natural products.Furthermore,this paper elaborates on the principles and applications of several …

Fabrication And Antioxidant Property Characterization Of Caffeic Acid Phenethyl Ester-Loaded Nanoparticles, Yaqiong Chen, Yumeng Li, Hui Wu, Hongdong Song, Xiao Guan

Journal of Food Science and Biotechnology

[Objective ] This study aims to improve the dispersion and utilization of caffeic acid phenethyl ester (CAPE ) in aqueous solutions.[Method ] CAPE-loaded hordein (CAPE-HD ) nanoparticles were constructed via an anti-solvent precipitation method.The physicochemical properties of the nanoparticles were characterized,and the antioxidant properties of the nanoparticles within Caco- 2 cells were investigated.[Result ] The components within the CAPE-HD nanoparticles interacted through non-covalent bonds,such as hydrogen bonding,resulting in an altered state of CAPE upon nanoparticle formation.The CAPE-HD nanoparticles exhibited near-spherical morphology with an average particle size of 193.93 nm,an average polydispersity index (PDI) of 0.26,and average Zeta potential of 14.50 …

Antimicrobial Resistance And Transmission Risk Of Foodborne Escherichia Coli In Wet Markets, Sisi Li, Wei Zhou, Zhijin Zhou, Bin Wang, Chengjun Qiu, Zhangsheng Zhang, Qi Zhang, Yun Sun, Xu Xu, Tao Shen, Jianzhong Han, Daofeng Qu

Journal of Food Science and Biotechnology

[Objective ] To evaluate the antimicrobial resistance and transmission risk of foodborne Escherichia coli in wet markets,this study isolated E.coli from pork,aquatic products,vegetables,and wastewater collected from three wet markets in Hangzhou,Zhejiang.[Method ] The isolates were identified by 16S rRNA sequencing,and their antimicrobial resistance phenotypes were determined via the disk diffusion method.PCR was employed to detect antimicrobial resistance genes and integrons in the isolates.The antimicrobial resistance characteristics of E.coli were analyzed,and conjugation experiments were performed to assess the potential for horizontal transfer of antimicrobial resistance genes.Co-occurrence network analysis was conducted to evaluate the transmission risk of antimicrobial resistance among the isolates.[Result …

Establishment Of A Molecularly Imprinted Electrochemical Sensor Nh 2-Mwcnts@ 4-Fpba For The Detection Of Epidermal Growth Factor In Dairy Products, Ruyue Zhang, Yiwei Wang, Hongxiu Zeng, Bini Wang, Fuxin Zhang, Zhenquan Huo, Yufang Liu

Journal of Food Science and Biotechnology

[Objective ] This study aims to develop a rapid and accurate method for detecting epidermal growth factor (EGF ).[Method ] 4-Formylphenylboronic acid (4-FPBA ) was covalently anchored to the surface of aminocarbon nanotubes (NH 2-MWCNTs ) to synthesize nanocomposites (NH 2-MWCNTs@ 4-FPBA ),and NH 2-MWCNTs@ 4-FPBA was modified on a glassy carbon electrode.When EGF was present,4-FPBA captured the EGF template through benzaldehyde,and then the electrode was immersed in pyrrole solution to electropolymerize to form a pyrrole molecularly imprinted layer.Finally,after dissociation and removal of the EGF template in an acidic solution,a three-dimensional cavity complementary to the EGF template was formed.[Result ] …

Immunomodulatory Effects And Marker Components Of Alcohol Extract From Acanthopanax Senticosus (Rupr . Et Maxim .) Harms- Ganoderma Applanatum (Pers.) Pat., Lihong Wang, Kairui Sheng, Ming Lu, Jin Su, Yang Ping, Yu Zhang, Ji Zhao, Yixia Gong

Journal of Food Science and Biotechnology

[Objective ] The aim of this study was to analyze the compositional changes of the alcohol extracts of Acanthopanax senticosus (Rupr.et Maxim.) Harms (APS )-Ganoderma applanatum (Pers.) Pat.(GA) before and after compounding at a mass ratio of 5∶1 (mAPS∶mGA=5∶1) and to investigate the immunomodulatory effects and marker components of the extracts.[Method ] The mouse models of compromised immunity and delayed-type hypersensitivity (DTH ) were established with cytoxan (CTX ) and 2,4-dinitrochlorobenzene (DNCB ),respectively,to investigate the effects of the alcohol extracts on the immune system.Ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS ) was used to analyze the changes of the active ingredients …

Effects And Differences Of (+)-Catechin And (-)-Epicatechin On Antioxidant Activity Of Hek 293t Cells, Chang Sun, Ning Xia, Chao Wang, Fengxian Qin, Chengyu Lyu, Jian Liu, Tiezhu Li

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effects and differences of the natural antioxidant (+)-catechin and its isomer (-)-epicatechin on the antioxidant activity of HEK 293T cells based on the nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE ) pathway.[Method ] The effects of (+)-catechin and (-)-epicatechin on the viability of HEK 293T cells were investigated via the methyl thiazolyl tetrazolium (MTT ) assay.Reporter genes were employed to investigate the regulatory effects on the activation of ARE.The effects of (+)-catechin and (-)-epicatechin on the mRNA and proteins levels of the downstream factors [glutamate-cysteine ligase modifier subunit (GCLM …

Effects Of Α-Amylase Addition On The Characteristics Of Infant Rice Flour, Huihui Liu, Zhiyong Dai, Mengyi Li, Jiao Peng, Xing Meng, Luyan Liao, Rongbin Zhang

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effects of α-amylase addition on the characteristics of infant rice flour.[Method ] Infant rice flour samples with different α-amylase addition were prepared.The basic composition,relative molecular mass of starch,gelatinization properties,hydration properties,texture properties,in vitro digestion characteristics,and microstructure of infant rice flour were determined.Furthermore,the correlations between the physicochemical characteristics and quality characteristics of infant rice flour were analyzed.[Result ] The weight-averaged molecular weight and the number-averaged molecular weight of starch in infant rice flour decreased with the increase in α-amylase addition.The gelatinization curve of infant rice flour with α-amylase addition was relatively gentle,and the gelatinization …

Effects Of Shaping And Baking Processes On The Antioxidant Activity Of Round Roasted Green Tea, Jun Liu, Gong Feng, Rong Tan, Weihong Huang

Journal of Food Science and Biotechnology

[Objective ] This study investigated the effects of shaping and baking processes on the antioxidant activity of round roasted green tea,aiming to provide theoretical support and practical reference for improving the quality and optimizing the processing techniques of green tea.[Method ] The influences of different processing stages and key procedures such as shaping and baking on the antioxidant activity of round roasted green tea were studied.A zebrafish model (in vivo ) and multiple indexes including DPPH radical scavenging rate,ABTS ⁺ radical scavenging rate,and ferric ion reducing antioxidant power (FRAP ) were employed for comprehensive evaluation of the antioxidant activity.[Result ] …

Effects And Mechanisms Of Phytosterol-Oil Bodies Emulsion On Lipid Metabolism Of Caenorhabditis Elegans, Jie Sun, Mengyuan Yuan, Yifei Liu, Fuyao Zhang, Zhuofan Yang, Guoyou Yin

Journal of Food Science and Biotechnology

[Objective ] This study aims to explore the lipid-lowering effects of the phytosterol-oil bodies emulsion (POE ).[Method ] Caenorhabditis elegans was taken as a model to evaluate the effects of POE on lipid metabolism,physiological indexes,and expression of genes associated with oxidative stress and fat metabolism.[Result ] Compared with the control group,different mass concentrations of POE reduced the mass fraction of fat (P<0.01) and the relative content of triglycerides (P<0.05),without toxicity to the growth and development of C.elegans (P<0.01).At the same time,it significantly reduced the level of reactive oxygen species and increased the levels of antioxidant enzymes.In addition,POE affected the expression levels of genes related to fat metabolism.qPCR results showed that POE up-regulated the mRNA level of sbp-1,while down-regulating the mRNA levels of fat-2,nhr-80,and fat-5 (P<0.05).[Conclusion ] POE may improve lipid metabolism by slowing fat deposition,regulating the fat peroxidation pathway,and alleviating oxidative stress in C.elegans.

The Rs1611770 Snp Polymorphism And Expression Of Mucin One Gene In Infertile Females In Baghdad, Iman Hamed Diab Al-Janabi, Asmaa M. Salih Almohaidi, Ibrahim Jasim Hammadi Al-Janabi

Baghdad Science Journal

Infertility is the inability to become pregnant after six months or more of consistent, unprotected sexual activity. There are several causes of infertility, including hormonal, genetic, and environmental factors. The current investigation focuses on the polymorphism of SNP rs 1611770 in Mucin one gene and expression in infertility, considering a few demographic variables including age, body mass index and circumference of the abdomen. The current study included 30 infertile females and 30 fertile females. The mean BMI recorded Significant differences 28.76 ± 0.85 for infertile and 25.99 ± 0.65 for fertile, There are no significant differences in age and abdominal …

State Of California Cannabis Reporting, University Of Mississippi – National Center For Cannabis Research And Education

California

No abstract provided.

Decoding The Future: African Parliaments, The 4ir, And The Ai Revolution, Bravin Onditi, Immanuel Mwendwa Kiilu

Young African Leaders Journal of Development

The Fourth Industrial Revolution (41R), propelled by advancements in Artificial Intelligence (AI), is ushering in a profound transformation across Africa's economies, governance systems, and societies. For African parliaments, there is an incredible challenge on the governance front, but the opportunities are unprecedented. This paper examines the range of biases, data insecurities, and inequalities the democracies of the continent face, while detailing how African legislatures can instate frameworks to govern and design AI function within the democracies of the continent. As seen in the examples of Rwanda, Kenya, South Africa, and Nigeria, this paper outlines the disparity between the astounding rates …

Zakat For Financing Sustainable Development In Africa: Opportunities And Challenges, Shawgei Salah Ahmed Ismaeil

Young African Leaders Journal of Development

The issue of Zakat and financing of SDGs has become an issue that economic, social and environmental researchers have raised, especially after the recent economic crisis. This is owing to Zakat dependence on the self-effort of societies and communities. Zakat supports the economic, social and environmental structure of societies. Africa, undoubtedly, needs to reduce poverty and hunger without preconditions by donors to change laws and the way of life of Africans. This task can be done through Islamic financing and Zakat. This paper puts this issue at the forefront of events for further study and research to rethink the practical …

Yaljod Full Issue 5.1, Sombo Muzata

Young African Leaders Journal of Development

This issue brings together a compelling collection of scholarly articles that reflect the dynamic intersection of ethics, technology, governance, and social justice in Africa's development discourse. The contributions span diverse sectors including artificial intelligence, education, agriculture, legal empowerment, and economic integration. The articles offer both critical insights and actionable frameworks as Africa continues to implement its African Union Agenda 2063 plan. This work, written by up-and-coming African scholars demonstrates the commitment to scholarship on African issues, focused on Africa by Africans. This has been our goal at the Young African Leaders Journal of Development i.e., to advance scholarship on pertinent …

Alirocumab In Dexamethasone-Induced Metabolic Syndrome: Therapeutic Benefits And Potential Hepatic Risks Through Rxr-Α / P-Akt Modulation, Miad A. Aljuhani, Mohamed S. Abdel-Bakky

Saudi Toxicology Journal

Background: Metabolic syndrome (MetS) elevates the risk of developing cardi-ovascular diseases and type 2 diabetes., driven by insulin resistance (IR), inflammation, and metabolic dysregulation. The PI3K/AKT pathway shows a key part in glucose and lipid metabolism. Alirocumab, a PCSK9 inhibitor, lowers LDL-C and improves IR but may cause hepatotoxicity. Aims: This study examines ali-rocumab’s effects on dexamethasone-induced MetS in rats, focusing on its metabolic benefits and potential liver toxicity. Methods: Over 30 days, 40 male Albino rats were distributed into control, Alirocumab, Dexamethasone, and Dexa-methasone+Alirocumab groups, with the latter receiving Dexamethasone to induce metabolic syndrome followed by Alirocumab administration. Results: …

Real-World Evidence For Herbal Medicine Benefit In 9728 Type 2 Diabetes Patients-Peridonotitis Risk And Ambulatory Care Utilization., Wen-Wei Lin, Chieh-Tsung Yen, Hanoch Livneh, Hua-Lung Huang, Ming-Chi Lu, Wei-Jen Chen, Tzung-Yi Tsai

Leadership, Learning and Counseling Faculty Publications and Presentations

Recent evidence manifests that individuals with type 2 diabetes (T2D) are increasingly affected by oral disorders. Although medicinal herbs have shown promise in managing T2D, their benefit in managing periodontitis risk and subsequent healthcare utilization remains uncertain.

Quality Improvement Project: Impact Of An Onboarding Program On Novice Nursing Clinical Adjunct Faculty Self-Efficacy, Tonie Metheny

Doctor of Nursing Practice Projects

Many nursing colleges rely on adjunct faculty to deliver the clinical curriculum (i.e., skills lab, clinical instruction, simulation, etc). Without a structured approach to orienting novice adjunct clinical nursing faculty to their roles and responsibilities, educational outcomes may be negatively impacted. Nursing literature documents the impact of a structured orientation program on novice self-efficacy. The purpose of this quality improvement project is to evaluate the impact of an orientation program on novice nursing adjunct clinical faculty self-efficacy with the Academic Clinical Nurse Educator (ACNE) core competencies. Self-efficacy was assessed using the Academic Clinical Nurse Educator Skills Acquisition Tool (ACNESAT). The …

Fetal Microchimerism Of The Eye Lens, Jamie Moubarak

Electronic Theses and Dissertations

Fetal microchimerism (FMc) is the presence of a small fetal cell population in the mother, sometimes exhibiting stem cell–like properties. FMc has been observed within many placental mammal organs, except the eye. We used Yspecific PCR on genomic mouse DNA to test the hypothesis that fetal microchimerism can also be found in the lens. Of the parous females tested, we detected an Ssty2-specific amplicon in 83% (5/6) of samples and an Sry-specific amplicon in 2/4 samples. 100% (5/5) of non-age-matched parous females were positive for both primers. These results provide evidence that cells of fetal or placental origin …

Generation And Characterization Of A Knockout Mouse Of An Enhancer Of Ebf3, Emily Cordova Hurtado, Janine M Wotton, Alexander Gulka, Crystal Burke, Jeffrey K Ng, Ibrahim Bah, Juana Manuel, Hillary Heins, Stephen A Murray, David U Gorkin, Jacqueline K White, Kevin A Peterson, Tychele N Turner

2020-Current year OA Pubs

Genomic studies of neurodevelopmental disorders (NDDs) have identified several relevant genomic variants. EBF3 is a gene with an excess of protein-coding de novo variants and underlies Hypotonia, Ataxia, and Delayed Development Syndrome. We previously identified noncoding de novo variants in an enhancer of EBF3 and further found enrichment of deletions of this enhancer in NDDs. In this study, we generated a novel mouse line that deletes the highly conserved, orthologous mouse region within the Rr169617 regulatory region, and characterized the molecular and phenotypic aspects of this mouse model. We found a deviation from Mendelian expectation (P=0.02) with significant depletion of …

Early Visual Predictors Of Progression To Alzheimer’S Disease And Dementia With Lewy Bodies, Abril Baez

Master's Theses

We examined whether specific visual cognitive skills predict progression from Mild Cognitive Impairment (MCI) to Alzheimer’s disease (AD), or dementia with Lewy bodies (DLB). The Benson Complex Figure Task (BCFT) was used to measure visual perception and construction (copy), and visual memory (recall). National Alzheimer Coordination Center longitudinal dataset included participants who remained stable (MCI-stable; n =2630), progressed to AD (MCI-AD; n =798), or progressed to DLB (MCI-DLB; n =59). Baseline BCFT scores were entered into logistic regression models predicting diagnostic outcome. Impaired copy performance was significantly associated with MCI-DLB (B = 0.79, OR = 2.21, BCa CI [0.26, 1.36], …

The Effect Of Childhood Psychological Abuse On Depressive Symptoms In Adolescents Exposed To Campus Suicide: The Chain Mediating Role Of Psychological Trauma And Anxiety Symptoms, Tingting Tan, Jiawei Zhao, Mengxuan Wu, Xinyue Zhang, Xinchun Liu, Lili Zhang, Jie Wu

Communication Sciences & Disorders Faculty Publications

Exposure to campus suicide poses a significant threat to adolescent mental health. While childhood psychological abuse (CPA) is a known vulnerability factor for depression, the mechanisms linking this early adversity to depressive symptoms (DS) following acute trauma remain unclear. This study aimed to test a chain mediation model where CPA contributes to DS through the sequential effects of psychological trauma (PT) and anxiety symptoms (AS). In a cross-sectional study of 1603 adolescents exposed to a campus suicide event, participants completed self-report measures for CPA, PT, AS, and DS. Chain mediation analysis revealed a significant direct effect of CPA on DS. …

The Transitional Human Being: Literary, Journalistic, And Cinematographic Representations Of The Twentieth- And The Twenty-First-Centuries Italy, Ron Kubati

Purdue Studies in Romance Literatures

This book analyzes the crisis of the idea of human being based on timeless notions, in the Euroamerican context, as seen primarily through literature and cinema. It focuses on Italy, which has been and remains a crossroads for diverse cultural and political currents—open to Mediterranean cultural influences while remaining within a typically Western cultural context. The “new man,” defined by Corrado Alvaro during his trip in Russia in 1934 as “transitional,” implies a human being with a fundamental need for change. Instead of the “new man,” Italo Calvino in the 1960 preface to his I nostri antenati wrote about the …

Turfgrass Weed Control For Professionals, 2024, Aaron J. Patton, Matthew T. Elmore

Purdue University Press Books

Turfgrass is used on everything from athletic fields, golf courses, and parks, to lawns, sod farms, and roadsides. Unfortunately, information on weed management for professionals maintaining these areas has often been inaccurate or out of date, and applicators are frequently unaware of the most effective methods for controlling weeds in turf. Written by experts in the field and featuring insights from contributors from sixteen states across the US, the 2024 edition of Turfgrass Weed Control for Professionals was developed to provide science-based information for those working in the field. It contains information about turfgrass management, weed biology and management, more …

Effects Of Residual Pah Exposure From Firefighters’ Skin And Turnout Gear On Biospecimen Microrna Expression, Jooyeon Hwang, Jenny R Gipson, Chao Xu, Timothy Vanwagoner, Xin Xu, Robert J Agnew, Willard M Freeman

Faculty, Staff and Student Publications

Firefighters are routinely exposed to polycyclic aromatic hydrocarbons (PAHs) during fire suppression activities, molecular biomarkers reflecting such exposure remain underexplored. Thus, we explored an integrated exposure assessment framework linking PAH contamination on turnout gear and skin to changes in microRNA (miRNA) expression in skin and blood biospecimens. Our goals are to evaluate the relationship between fire-related PAH exposure and miRNA expression profiles in firefighters, and to identify candidate miRNA biomarkers of occupational exposure. This prospective study enrolled 25 firefighters. PAHs on turnout gear and skin were quantified post-fire using gas chromatography/mass spectrometry. Concurrently, skin tape strips and blood were collected …

Imaging Features Of High-Grade Astrocytoma With Piloid Features: A Single Center Case Series, Heba Al Qudah, Jackson D Hamilton, Maria A Gubbiotti, Ahmed Msherghi, Hamza A Salim, Sahar Alizada, Ho-Ling Liu, Vinodh A Kumar, Max Wintermark, Rami W Eldaya

Faculty, Staff and Student Publications

High-grade astrocytoma with piloid features (HGAP) is a recently described IDH-wildtype tumor with limited literature describing its imaging features. We present our institution's experience and the largest imaging-focused cohort of HGAP reported to date, offering a comprehensive analysis of MRI features of seventeen intracranial and one spinal HGAP, while introducing novel imaging features that have not been previously described. These include focal areas of diffusion restriction, surrounding spiculated, finger-like enhancement and increased perfusion metrics. Additionally, we highlight imaging features of HGAP arising in patients with Neurofibromatosis Type 1, such as intraventricular tumor spread, unusual temporal lobe location and multifocal presentation.

Advancements In Understanding Spasticity: A Neuromusculoskeletal Modeling Perspective, Mohammad S Shourijeh, Argyrios Stampas, Shuo-Hsiu Chang, Radha Korupolu, Gerard E Francisco

Faculty, Staff and Student Publications

Spasticity, a complex consequence of upper motor neuron lesions, poses challenges for clinical assessment due to its neural and mechanical origins. Traditional scales like the Modified Ashworth and Tardieu Scales provide subjective, context-limited insights, often missing spasticity's dynamic nature. Neuromusculoskeletal (NMS) modeling offers objective, quantitative insights by integrating patient-specific muscle-tendon properties, reflex dynamics, and multi-joint biomechanics. This scoping review examines advancements in spasticity modeling, comparing mechanical, neurological, and integrated approaches, and their applications in conditions like cerebral palsy and stroke. We highlight barriers to clinical translation, including computational demands and regulatory challenges, and propose future directions, such as real-time simulation …

Late Morbidity And Mortality In Survivors Of Childhood Ependymoma: A Report From The Childhood Cancer Survivor Study (Ccss), Katharine R Lange, Peter De Blank, Mengqi Xing, Sedigheh Mirzaei, Deo Kumar Srivastava, Kevin Oeffinger, Joseph Neglia, Kevin Krull, Paul C Nathan, Rebecca Howell, Kirsten K Ness, Lucie M Turcotte, Wendy Leisenring, Gregory T Armstrong, Tara Brinkman, Daniel C Bowers, Mehmet Fatih Okcu

Faculty, Staff and Student Publications

Background/objectives: Treatment of childhood ependymoma evolved from 1970 to 1999 by reducing radiation volumes and incorporating chemotherapy. The impact of these changes on long-term health outcomes remains unknown. In this report, we evaluated temporal changes in all-cause and cause-specific late mortality, chronic health conditions (CHCs), and subsequent neoplasms (SNs) in the Childhood Cancer Survivor Study (CCSS) cohort of adult survivors of pediatric ependymoma, diagnosed between 1970 and 1999.

Methods: A total of 404 five-year survivors of ependymoma (47.5% female, 80.7% non-Hispanic White, median 6 (range 0-20) years at diagnosis, 22 (5-49) years from diagnosis) diagnosed between 1970 and 1999 and …