Digital Commons Network^™

Macroh2a Histone Variants Modulate Enhancer Activity To Repress Oncogenic Programs And Cellular Reprogramming, Wazim Mohammed Ismail, Amelia Mazzone, Flavia G Ghiraldini, Jagneet Kaur, Manvir Bains, Amik Munankarmy, Monique S Bagwell, Stephanie L Safgren, John Moore-Weiss, Marina Buciuc, Lynzie Shimp, Kelsey A Leach, Luis F Duarte, Chandandeep S Nagi, Saul Carcamo, Chi-Yeh Chung, Dan Hasson, Neda Dadgar, Jian Zhong, Jeong-Heon Lee, Fergus J Couch, Alexander Revzin, Tamas Ordog, Emily Bernstein, Alexandre Gaspar-Maia

Faculty, Staff and Students Publications

Considerable efforts have been made to characterize active enhancer elements, which can be annotated by accessible chromatin and H3 lysine 27 acetylation (H3K27ac). However, apart from poised enhancers that are observed in early stages of development and putative silencers, the functional significance of cis-regulatory elements lacking H3K27ac is poorly understood. Here we show that macroH2A histone variants mark a subset of enhancers in normal and cancer cells, which we coined 'macro-Bound Enhancers', that modulate enhancer activity. We find macroH2A variants localized at enhancer elements that are devoid of H3K27ac in a cell type-specific manner, indicating a role for macroH2A at …

Montana Kaimin, February 23, 2023, Students Of The University Of Montana, Missoula

Montana Kaimin, 1898-present

Student newspaper of the University of Montana, Missoula.

Child Adult Relationship Enhancement In Primary Care (Pricare): Study Design/Protocol For A Randomized Trial Of A Primary Care-Based Group Parenting Intervention To Prevent Child Maltreatment, Samantha Schilling, Byron J Powell, Paul W Stewart, Joanne N Wood

2020-Current year OA Pubs

BACKGROUND: Child maltreatment (CM) is a pervasive public health problem and there is a critical need for brief, effective, scalable prevention programs. Problematic parent-child relationships lie at the heart of CM. Parents who maltreat their children are more likely to have punitive parenting styles characterized by high rates of negative interaction and ineffective discipline strategies with over-reliance on punishment. Thus, parenting interventions that strengthen parent-child relationships, teach positive discipline techniques, decrease harsh parenting, and decrease child behavioral problems hold promise as CM prevention strategies. Challenges in engaging parents, particularly low-income and minority parents, and a lack of knowledge regarding effective …

[18f]Tosyl Fluoride As A Versatile [18f]Fluoride Source For The Preparation Of 18f-Labeled Radiopharmaceuticals, Dong Zhou, Wenhua Chu, Jinbin Xu, Sally Schwarz, John A Katzenellenbogen

2020-Current year OA Pubs

Positron emission tomography (PET) is an in vivo imaging technology that utilizes positron-emitting radioisotope-labeled compounds as PET radiotracers that are commonly used in clinic and in various research areas, including oncology, cardiology, and neurology. Fluorine-18 is the most widely used PET-radionuclide and commonly produced by proton bombardment of

Genetic Subgroups Inform On Pathobiology In Adult And Pediatric Burkitt Lymphoma, Nicole Thomas, Nancy L. Bartlett, Lee Ratner, Et Al.

2020-Current year OA Pubs

Burkitt lymphoma (BL) accounts for most pediatric non-Hodgkin lymphomas, being less common but significantly more lethal when diagnosed in adults. Much of the knowledge of the genetics of BL thus far has originated from the study of pediatric BL (pBL), leaving its relationship to adult BL (aBL) and other adult lymphomas not fully explored. We sought to more thoroughly identify the somatic changes that underlie lymphomagenesis in aBL and any molecular features that associate with clinical disparities within and between pBL and aBL. Through comprehensive whole-genome sequencing of 230 BL and 295 diffuse large B-cell lymphoma (DLBCL) tumors, we identified …

Allogeneic Natural Killer Cell Therapy, Melissa M Berrien-Elliott, Miriam T Jacobs, Todd A Fehniger

2020-Current year OA Pubs

Interest in adoptive cell therapy for treating cancer is exploding owing to early clinical successes of autologous chimeric antigen receptor (CAR) T lymphocyte therapy. However, limitations using T cells and autologous cell products are apparent as they (1) take weeks to generate, (2) utilize a 1:1 donor-to-patient model, (3) are expensive, and (4) are prone to heterogeneity and manufacturing failures. CAR T cells are also associated with significant toxicities, including cytokine release syndrome, immune effector cell-associated neurotoxicity syndrome, and prolonged cytopenias. To overcome these issues, natural killer (NK) cells are being explored as an alternative cell source for allogeneic cell …

Level 2 And Level 3 Patients In A 5-Level Triage System: Factors Related To Acuity Assignment And Trajectory Of The Emergency Department Experience, Roxanne O. Garbez

Dissertations

Clinical decision making in the emergency department (ED) can have a significant impact on patient safety and health care outcomes. One type of clinical decision making is the process of emergency department triage. Within the context of the 5-level Emergency Severity Index triage system, it is not known what factors influence patient assignment by the triage nurse to ESI acuity levels 2 and 3. To increase the validity of patient assignment to levels 2 and 3, more explicit criteria are needed.

Purposes: (1) Describe factors that influence triage nurse assignment of patients to level 2 and level 3; (2) Examine …

Developing A Standardized But Extendable Framework To Increase The Findability Of Infectious Disease Datasets, Ginger Tsueng, Marco A Alvarado Cano, José Bento, Candice Czech, Mengjia Kang, Lars Pache, Luke V Rasmussen, Tor C Savidge, Justin Starren, Qinglong Wu, Jiwen Xin, Michael R Yeaman, Xinghua Zhou, Andrew I Su, Chunlei Wu, Liliana Brown, Reed S Shabman, Laura D Hughes

Faculty, Staff and Students Publications

Biomedical datasets are increasing in size, stored in many repositories, and face challenges in FAIRness (findability, accessibility, interoperability, reusability). As a Consortium of infectious disease researchers from 15 Centers, we aim to adopt open science practices to promote transparency, encourage reproducibility, and accelerate research advances through data reuse. To improve FAIRness of our datasets and computational tools, we evaluated metadata standards across established biomedical data repositories. The vast majority do not adhere to a single standard, such as Schema.org, which is widely-adopted by generalist repositories. Consequently, datasets in these repositories are not findable in aggregation projects like Google Dataset Search. …

February 23, 2023, The Daily Mississippian

Daily Mississippian (all digitized issues)

No abstract provided.

Intellectual Disability Related To De Novo Germline Loss Of The Distal End Of The P-Arm Of Chromosome 17: A Case Report, Eden Pope, Matthew Huertas, Amar Paul, Braden Cunningham, Matthew Jennings, Ryan Perry, Stephanie Chavez, John A. Kriak, Kyle B. Bills, David W. Sant

Annual Research Symposium

Hypothesis/Purpose: In this report we present a case of a 20-year-old female with congenital intellectual disability, stunted growth, and hypothyroidism. Competitive genetic hybridization (CHG) revealed a loss of 17p13.3, and the deletion was not present in either parent. This deletion has not previously been characterized, but mutations on the p-arm of chromosome 17 are responsible for Miller-Dieker Syndrome and Isolated Lissencephaly Sequence, both of which share symptoms in common with the patient.

Methods: Peripheral mononuclear cells (PBMCs) were used for karyotyping and competitive genetic hybridization (CHG). Bioinformatic analysis was carried out using the Genome Data Viewer (ncbi.nlm.nih.gov/genome/gdv).

Results: Karyotype was …

The Heart Rates And Movement Speed Of Specialist Tactical Police During A Multistorey Active Shooter Training Scenario, Jeremy Robinson, Mihajlo Micovic, Ben Schram, Alexander Leroux, Robin Marc Orr

International Journal of Exercise Science

International Journal of Exercise Science 16(4): 281-292, 2023. Specialist police tactical groups (PTGs) are involved in situations that extend beyond the capability of general duties police and can include responding to active shooter incidents. Due to the nature of their tasks, these officers typically carry and wear additional equipment which can impart an increased physical cost, for which the officers must be prepared. The aim of this study was to examine the heart rate responses and movement speeds of specialist PTG officers during a multistorey active shooter scenario. Eight PTG officers completed an active shooter scenario within a multistorey office …

Can Multidetector Ct Scan Replace Mri For Evaluation Of Mesorectal Fasca In Rectal Cancer?, Maryam Farghdani, Mehdi Karami, Amirafraz Fallah

BioMedicine

Abstract

Background

Colorectal cancer is the 4th leading cause of cancer mortality.

Aim

This study aimed to can multidetector CT scan (MDCT) replace Magnetic resonance imaging (MRI) for mesorectal fasca in rectal cancer?

Methods

This cross-sectional was conducted on patients with rectal cancer in Alzahra and Seyed-Shohada hospitals. MRI, mesorectal fascia (MPR), and axial MDCT were provided from patients to assess the involvement of mesorectal fascia. Sensitivity, specificity, positive and negative predictive values were assessed in these patients. Kappa coefficient agreement was used for analysis. Patients were also divided into different subgroups based on gender, tumoral location, nodal involvement, and …

A1 Adenosine Receptor Antagonist Induces Cell Apoptosis In Kyse-30 And Ym-1 Esophageal Cancer Cell Lines, Parisa Zeynali, Marie Saghaeian Jazi, Jahanbakhsh Asadi, Seyyed Mehdi Jafari

BioMedicine

Background and Aim: Adenosine A1 receptor (AA1R) has been shown to have an inhibitory effect on cell growth in several cancers; however, its function in esophageal cancer is still unclear. In this study, we examined the effect of AA1R on cell growth and apoptosis in esophageal cancer cells. Materials and methods: In this study, YM-1 and KYSE-30 esophageal cancer cell lines were cultured. AA1R gene expression was determined by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). As well, the AA1R antagonist (DPCPX) effect on cell viability was evaluated by the MTT assay. Moreover, apoptosis was assessed by annexin-V and propidium iodide …

Jugular Foramen Versus Hypoglossal Canal In Axial Ct Scan, Maryam Mohammadzadeh, Reza Erfanian, Saman Rezaeian, Nasim Batavani, Behrooz Amirzargar

BioMedicine

Background: Differentiating jugular foramen from hypoglossal canal in computed tomography (CT) scan is vital for correct diagnosis of posterior fossa pathologies; however, it has been shown that the ability for differentiating these skull base elements is limited. The purpose of this study was to produce a simple algorithm for differentiating the jugular foramen from the hypoglossal canal in axial CT scan on two levels (top level where bony carotid canal is evident and lower level where bony carotid canal is not evident). Methods: Data derived from axial CT scan of 250 patients (500 sides) were used for producing algorithm. At …

The Impact Of Procalcitonin In Assessing Outcomes In Pediatrics Severe Trauma Cases: A Three-Year Experience From A Tertiary Hospital, Waleed H. Albuali

BioMedicine

Background Although procalcitonin levels are raised in patients with systemic inflammation, its usage in pediatric patients, particularly those in the intensive care unit who are most susceptible to sepsis. Methods It is a retrospective research study that included pediatric patients aged more than two weeks who were brought to the King Fahd Hospital of the University's PICU owing to serious trauma or post-acute postoperative occurrences from January 2017 to December 2019. At 24 hours after admission, data such as age, gender, comorbidities, trauma severity as measured by the Injury Severity Score, and PRISM III score were collected. Results Following a …

High Levels Of Histone H3 K27 Acetylation And Tri-Methylation Are Associated With Shorter Survival In Oral Squamous Cell Carcinoma Patients, Akram Shahhosseini, Ekaterina Bourova-Flin, Samira Derakhshan, Pouyan Aminishakib, Afsaneh Goudarzi

BioMedicine

Background: Acetylation and trimethylation of histone H3 lysine 27 (H3K27ac and H3K27me3) generally activate and repress transcription, respectively. Concurrent activation of H3K27ac and H3K27me3 has been reported to correlate with poor prognosis in hepatocellular carcinoma. A high level of H3K27me3 has been shown to be associated with advanced oral squamous cell carcinoma (OSCC) tumour stage, but prognostic impact of H3K27ac level alone/or in combination with H3K27me3 in OSCC patients had not yet been reported. Material and Methods: In this study, immunohistochemistry using specific antibodies against H3K27ac and H3K27me3 was performed on a series of 72 OSCC samples to investigate the …

Effects Of Hepatocyte Growth Factor On Porcine Mammary Cell Growth And Senescence, Chi-Ping Huang, Liang-Chih Liu, Hsin-Ling Lu, Chih-Rong Shyr

BioMedicine

Background: The porcine mammary glands share morphological and physiological similarities with human ones, making primary porcine mammary cells (PMC) suitable for biomedical research and a potential cellular therapeutic for breast cancer xenogeneic cell immunotherapy. Primary cells isolated from tissues remain the physiological functions of origin tissues but their self-renewal ability is restricted and cells acquire senescence during in vitro expansion. To overcome these drawbacks, here we sought to establish an approach to efficiently increase PMC’s in vitro growth. We studied the effects of the hepatocyte growth factor (HGF) to maintain the expansion capacity of porcine mammary cells and identify the …

New-Onset Diabetes In Covid-19: The Molecular Pathogenesis, Desak Made Wihandani, Made Lady Adelaida Purwanta, W. Riski Widya Mulyani, I Wayan Ardyan Sudharta Putra, I Gede Putu Supadmanaba

BioMedicine

Diabetes mellitus (DM) is still a challenging metabolic disease worldwide. In the current situation, the world is facing a COVID-19 pandemic due to SARS-CoV-2 infection. DM is one of comorbid that can worsen the severity of the COVID-19 condition. Surprisingly, SARS-CoV-2 infection can induce new-onset diabetes, a condition in which acute hyperglycemia occurs and may potentially develop into a complication in non-diabetic patients. Angiotensin-converting enzyme 2 (ACE2) is a crucial entry factor of SARS-CoV-2 infection. ACE2 will bind to the spike protein of SARS-CoV-2, making its entrance to the potentially damaging process of many tissues in the human body, including …

Beyond Mental Stress-Induced Myocardial Ischemia Following The Covid-19 Vaccine, Chih-Cheng Lai, Po-Ren Hsueh

BioMedicine

No abstract provided.

Shatt Al-Arab River And The Seawater Intrusion: Causes And Solutions, Havan H. Salman, Hamdiea Skheel Jazaa

Al-Bahir

. The scarcity and pollution of water resources led to environmental deterioration in Iraq, where the water needs for agricultural purposes are estimated at about 51 billion m3 annually to irrigate 11.3 million dunums and about 10 billion m3 annually for social health needs and 5 billion m3 annually for industrial, oil needs and electricity production. This paper addresses another factor exacerbating the crisis and posing another challenge to competition, which is pollution from seawater intrusion. Take the Shatt al-Arab as a case study. Shatt al-Arab is the main water source for the city of Basra in southern Iraq, where …

The Corona Virus Pandemic Psychological Effect And Its Relationship To Future Anxiety Among Teachers, Ahmad Al- Shraifin, Ali Jarwan, Mu’Ayyad Megdadi

Hebron University Research Journal-B (Humanities) - (مجلة جامعة الخليل للبحوث- ب (العلوم الانسانيه

The study aimed at revealing the relation between Corona virus pandemic psychological effect and future anxiety among teachers considering some variables (i.e., gender, social status, and school type). To achieve the aims of the study, the Impact of Event Scale-Revised IES-R scale and the future anxiety scale were utilized, after examining their validity and reliability. The sample consisted of 254 teachers from public and private schools in Irbid. The results show that there are statistically significant differences in the means of corona virus pandemic psychological effect scale due to the type of school; private school teachers were more affected by …

Academic Motivation Level In The Light Of Corona Virus Pandemic Among Secondary School Students In Irbid City Due To Some Variables (Gender And School Branch), Samhan Alabedly

Hebron University Research Journal-B (Humanities) - (مجلة جامعة الخليل للبحوث- ب (العلوم الانسانيه

This study aimed to reveal the academic motivation level in the light of COVID-19 pandemic among secondary school students in Irbid city due to some variables (gender and school branch). To achieve the study objectives, the descriptive survey method was used through preparing an electronic questionnaire consisting of (27) items divided into two domains (Intrinsic and Extrinsic Motivation) to reveal the academic motivation level. The study sample consisted of (456) students, of whom (187) and (269) female students who were selected by the available method. The study results showed that the academic motivation level among secondary school students was moderate. …

The Relationship Of Future Anxiety With Life Quality Among A Sample Of Widows In Irbid Governorat, Amna Hekmat Khasawneh

Hebron University Research Journal-B (Humanities) - (مجلة جامعة الخليل للبحوث- ب (العلوم الانسانيه

The study aimed to reveal the level of both future anxiety and quality of life, and the relationship between them among a sample of widows in Irbid Governorate. To achieve the objectives of the study, the researcher used the future anxiety scale consisted of (32) items in five domains (psychological, healthy, social, family, economic), and the quality of life scale consisted of (27) items. The study sample consisted of (377) registered widows in community services centres, family and women's protection and support centres located in various districts in Irbid Governorate. The results of the study showed that the level of …

Body Language Skills And Their Relationship To Professional Performance Among Educational Counselors In The West Bank Governorates, Maysaa Al-Tarif, Iyad Abu Bakr

Hebron University Research Journal-B (Humanities) - (مجلة جامعة الخليل للبحوث- ب (العلوم الانسانيه

This study aimed to explore the relationship between the use of body language skills in the professional performance of educational counsellors in the governorates of the West Bank (Palestine). The correlational descriptive approach was used, and the sample of the study consisted of (190) educational counsellors (males and females) in the schools of the educational directorates in the West Bank. The study was carried out during the scholastic year (2019/2020), the sample was chosen by the simplified sampling method. To achieve the study objectives, the two study tools were developed, and the results showed that there was a positive correlation …

The Big Five Personality Traits As A Predictors Of The Social Intelligence Among Students Of The Faculty Of Educational Sciences And Arts / Unrwa, Nafez Bukaiei, Mohammad Al-Absi

Hebron University Research Journal-B (Humanities) - (مجلة جامعة الخليل للبحوث- ب (العلوم الانسانيه

The study aimed to find out the predictive ability of the big five personality traits in social intelligence among students of the Faculty of Educational Sciences and Arts/ UNRWA. The sample of the study consisted of (202) male and female students, who were chosen by the random stratified method. To answer the study questions, the correlation coefficient and the multiple regression analysis were used. The results also showed the correlation relationship between all personality factors and social intelligence, with the exception of the conscientiousness factor, and the results also showed some differences in the correlation relationship between the big five …

We Are ... Marshall, February 22, 2023, Office Of Marshall University Communications

We Are ... Marshall: the Newsletter for Marshall University 1999-2023

No abstract provided.

Statement Of The District Task Force On Jails And Justice Before The Committee On The Judiciary And Public Safety Of The Council Of The District Of Columbia. Performance Oversight Hearing For The Deputy Mayor For Public Safety & Justice, Katherine S. Broderick

D.C. Council Testimony

No abstract provided.

2023-02-22 Michiganoverview_Covidbedppe, Michigan Department Of Health & Human Services

Michigan COVID-19 Hospital Data

Contains the tables Statewide Hospital Capacity Report for COVID-19, COVID-19 Metrics, Statewide Hospital Available PPE Tracking for COVID-19, as well as the tables PPE Days On Hand and Patient Census.

The Minutes Of The Marshall University Board Of Governors Meeting, February 22, 2023, Marshall University Board Of Governors

Board of Governors Minutes

No abstract provided.

Spartan Daily, February 22, 2023, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2023

Volume 160, Issue 12