Digital Commons Network^™

Regulation And Function Of Pp2a-B56delta And Its Pathological Variants, Kali A. Smolen

Dartmouth College Ph.D Dissertations

B56δ is a regulatory subunit of protein phosphatase PP2A, which is responsible for the majority of phosphoserine and phosphothreonine dephosphorylation within a cell. PPP2R5D-Related Developmental Disorder is characterized by intellectual disability, autism-spectrum disorder, hypotonia, and epilepsy and is caused by de novo single nucleotide changes in PPP2R5D, the gene that encodes B56δ, resulting in missense mutations (i.e. E198K, E420K, etc.). Yet, the molecular mechanisms impacted by these variants are unknown. To investigate these mechanisms as described in Chapters 2 and 3, we generated HEK293 cells harboring a single nucleotide change in the endogenous locus of PPP2R5D to recapitulate two pathogenic …

Caging The Bored Ape: How The Ftc's Expanded Anti-Monopoly Authority Can Tame "Nfts" For Web 3.0, J. Scott Colesanti

Hofstra Law Faculty Scholarship

Non-Fungible Tokens, or "NFTs," ballooned into a 40-billion-dollar industry in under a decade. Their creators include artists, corporations, entrepreneurs, fraudsters--and even Donald Trump. While NFT owners and traders could be any of us, the parties running the marketplaces are hidden. NFT regulators have yet to be identified. Most alarmingly, the dominant NFT marketplaces are dangerously centralized. Accordingly, the publicized tales of exorbitant or manipulated NFT prices and frequent related scams abound. Meanwhile cryptocurrency--the technology enabling the life of an NFT--remains beset with, at best, theoretical models for effective regulation a full generation after its emergence. To propose a rational start …

Clinical Benefit From Docetaxel +/- Ramucirumab Is Not Associated With Mutation Status In Metastatic Non-Small-Cell Lung Cancer Patients Who Progressed On Platinum Doublets And Immunotherapy, Kang Qin, Kaiwen Wang, Shenduo Li, Lingzhi Hong, Priyadharshini Padmakumar, Rinsurongkawong Waree, Shawna M Hubert, Xiuning Le, Natalie Vokes, Kunal Rai, Ara Vaporciyan, Don L Gibbons, John V Heymach, J Jack Lee, Scott E Woodman, Caroline Chung, David A Jaffray, Mehmet Altan, Yanyan Lou, Jianjun Zhang

Faculty, Staff and Student Publications

Docetaxel +/- ramucirumab remains the standard-of-care therapy for patients with metastatic non-small-cell lung cancer (NSCLC) after progression on platinum doublets and immune checkpoint inhibitors (ICIs). The aim of our study was to investigate whether the cancer gene mutation status was associated with clinical benefits from docetaxel +/- ramucirumab. We also investigated whether platinum/taxane-based regimens offered a better clinical benefit in this patient population. A total of 454 patients were analyzed (docetaxel +/- ramucirumab n=381; platinum/taxane-based regimens n=73). Progression-free survival (PFS) and overall survival (OS) were compared among different subpopulations with different cancer gene mutations and between patients who received docetaxel …

Should We Stent Vulnerable, But Asymptomatic, Lesions?, Nils P Johnson, K Lance Gould, Jagat Narula

Faculty, Staff and Student Publications

No abstract provided.

Characteristics Of Early Pancreatic Cancer: Comparison Between Stage 1a And Stage 1b Pancreatic Cancer In Multicenter Clinical Data Warehouse Study, Hyo Suk Kim, Young Hoon Choi, Jae Sin Lee, Ik Hyun Jo, Sung Woo Ko, Kyu Hyun Paik, Hyun Ho Choi, Han Hee Lee, Yeon Soo Lim, Chang Nyol Paik, In Seok Lee, Jae Hyuck Chang

Faculty, Staff and Student Publications

BACKGROUND: Little is known about the characteristics of early pancreatic cancer. We aimed to identify the characteristics, clues for early detection, and prognostic factors for early pancreatic cancer by analyzing a large number of patients with stage 1 pancreatic cancer.

METHODS: A clinical data warehouse that includes databases of all the medical records of eight academic institutions was used to select and analyze patients with pancreatic cancer that had been diagnosed from January 2010 to May 2023.

RESULTS: In total, 257 stage 1 pancreatic cancer patients were included. There were 134 men (52%), and the average age was 67.2 ± …

Comparing Anesthesia And Surgery Controlled Time For Primary Total Knee And Hip Arthroplasty Between An Academic Medical Center And A Community Hospital: Retrospective Cohort Study, Thy B Nguyen, Nathaen Weitzel, Craig Hogan, Rachel M Kacmar, Kayla M Williamson, Jack Pattee, Vesna Jevtovic-Todorovic, Colby G Simmons, Adeel Ahmad Faruki

Faculty, Staff and Student Publications

BACKGROUND: Osteoarthritis is a significant cause of disability, resulting in increased joint replacement surgeries and health care costs. Establishing benchmarks that more accurately predict surgical duration could help to decrease costs, maximize efficiency, and improve patient experience. We compared the anesthesia-controlled time (ACT) and surgery-controlled time (SCT) of primary total knee (TKA) and total hip arthroplasties (THA) between an academic medical center (AMC) and a community hospital (CH) for 2 orthopedic surgeons.

OBJECTIVE: This study aims to validate and compare benchmarking times for ACT and SCT in a single patient population at both an AMC and a CH.

METHODS: This …

The Future Of Targeted Therapy For Leiomyosarcoma, Ryan A Denu, Amanda M Dann, Emily Z Keung, Michael S Nakazawa, Elise F Nassif Haddad

Faculty, Staff and Student Publications

Leiomyosarcoma (LMS) is an aggressive subtype of soft tissue sarcoma that arises from smooth muscle cells, most commonly in the uterus and retroperitoneum. LMS is a heterogeneous disease with diverse clinical and molecular characteristics that have yet to be fully understood. Molecular profiling has uncovered possible targets amenable to treatment, though this has yet to translate into approved targeted therapies in LMS. This review will explore historic and recent findings from molecular profiling, highlight promising avenues of current investigation, and suggest possible future strategies to move toward the goal of molecularly matched treatment of LMS. We focus on targeting the …

Challenges To Inclusive Education For Students With Disabilities In Japanese Higher Education Institutions, Karina Dyliaeva, Steven B. Rothman, Nader Ghotbi

Higher Learning Research Communications

Objective: The purpose of the study was to elucidate the current challenges to inclusive education (IE) at the university level in Japan, thereby addressing the gap between policy and the provision of inclusion.

Method: This qualitative case study of a private university supporting inclusive policies in Japan included content analysis of data collected through semi-structured interviews to ascertain themes.

Results: The four identified themes were: inclusion practices as a conceptual challenge, conflicting practice of reasonable accommodations, inclusion management gaps, and barriers to and opportunities for inclusive education.

Conclusions: There is a significant disconnect between legal obligation and actual implementation of …

A Flexible Inventory Of Survey Items For Environmental Concepts Generated Via Special Attention To Content Validity And Item Response Theory, John A. Vucetich, Jeremy T. Bruskotter, Benjamin Ghasemi, Claire E. Rapp, Michael Paul Nelson, Kristina M. Slagle

Michigan Tech Publications

We demonstrate how many important measures of belief about the environmental suffer from poor content validity and inadequate conceptual breadth (dimensionality). We used scholarship in environmental science and philosophy to propose a list of 13 environmental concepts that can be held as beliefs. After precisely articulating the concepts, we developed 85 trial survey items that emphasized content validity for each concept. The concepts’ breadth and the items’ content validity were aided by scrutiny from 17 knowledgeable critics. We administered the trial items to 449 residents of the United States and used item response theory to reduce the 85 trial items …

Examining Speech-Language Pathology Research Relating To People With Traumatic Brain Injury In China: A Scoping Review, Guanyu Wei

Student Scholarship

Background/Aims Although there are many researchers reviewing the existing evidence related to traumatic brain injury from speech-language pathologists’ perspectives, there have been no attempts to review the existing research evidence in Standard Chinese to the best of the authors’ knowledge. The purpose of this scoping review was to identify and synthesise the existing research evidence available in China for therapy related to individuals with traumatic brain injury within the speech-language pathologists’ scope of practice. Methods This scoping review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews. The literature search was conducted using databases that …

Intensive Care Admission Aiming At Organ Donation. Not Sure, Anna Henrikje Seidlein, Jos M. Latour, Julie Benbenishty

School of Nursing and Midwifery

No abstract provided.

Inauthentic Inclusion: Exploring How Intention To Use Ai‐Generated Diverse Models Can Backfire, Sean Sands, Vlad Demsar, Carla Ferraro, Colin L. Campbell, Eli Cohen

School of Business: Faculty Scholarship

Rapid advances in AI technology have important implications for, and effects on, brands and advertisers. Increasingly, brands are creating digital models to showcase clothing and accessories in a similar way to human models, with AI used to customize various body types, ages, sizes, and skin tones. However, little is known about how the underrepresented consumers respond to a brand's intention to use AI-generated models to represent them. We explore this by conducting four studies. We find evidence that a brand's intention to use AI-generated (vs. human) models negatively affects brand attitude (study 1). We further investigate this effect using two …

Open-Window Craniectomy For The Removal Of Frontal Sinus Mucosa To Prevent A Delayed Mucocele: Illustrative Case, Michael Mcdermott

All Publications

Background: Frontal craniotomies for a medial subfrontal approach necessitate crossing the frontal sinus. Large superior extensions of the frontal sinus into frontal bone can result in mucosal retention in a free craniotomy bone flap, leading to a delayed mucocele with significant associated morbidity. The authors describe an "open-window" craniectomy technique that permits mucosal removal under direct vision and maintains the inner table on the bone flap's inferior side, helping to seal off the sinus opening with a pericranial flap.

Observations: An illustrative case involving a medial right frontal craniotomy for a third ventricle mass in a patient with a large …

An Examination Of Psychological Flexibility As A Mediator Between Mental Health Concerns And Satisfaction With Life Among Autistic Adults, Ty B. Aller, Heather H. Kelley, Tyson S. Barrett, Benjamin Covington, Michael E. Levin, Maryellen Brunson Mcclain

Psychology Faculty Publications

Background: Experiencing mental health concerns (e.g., depression, anxiety, stress) can have negative effects on satisfaction with life (SWL) for autistic adults. Current mental health supports that may promote SWL do not effectively meet the needs of autistic adults, often due to deficits-based approaches. Methods: To begin addressing the gap in research surrounding mental health supports among autistic adults, we designed a preregistered longitudinal study that used structural equation modeling to assess 289 autistic adults from the Simons Powering Autism Research (SPARK) program to determine if progress toward ones values, a strengths-based component of psychological flexibility, is a potential …

Isokinetic Versus Isotonic Resistance Training: Defining The Mechanisms Of Hypertrophy And Exercise Execution Methods, George Daughtry

Doctorate in Kinesiology

Abstract

Purpose: To determine strength changes and hypertrophy differences at the biceps brachii between an isokinetic bicep curl machine created by C&M Machines and preacher curl exercise with a standard curl bar. The study also aimed to determine the effectiveness of the aforementioned machine and to give insight into this unique method of training.

Methods: A medical clearance form and a Physical Activity Readiness Questionnaire was implemented to evaluate ability to exercise. 10 subjects (n=10) were recruited, were 20.3 years old (20 +/- 2), and were cleared for rigorous exercise. Subjects were randomly assigned to a control group …

Development Of Technology Pilot Training To Reduce The Number Of Senior Citizens Victimized By Computer Fraud Scams, James Eric Johnston

Walden Dissertations and Doctoral Studies

There is need in the public sector to help people 60+ years of age in the safe, practical use of technology for business, personal use, and family interaction. This age group is targeted more than others involving technology fraud. The current study focused on which topics need to be part of Technology Fraud Pilot Training to reduce the victimization that is put upon seniors 60+ years of age. The project included measures that may improve understanding and safe usage of basic technology that is frequently used. These searches came from public administration, technology, government, city government, state sources, and others …

Scgist: Gene Panel Design For Spatial Transcriptomics With Prioritized Gene Sets, Mashrur Ahmed Yafi, Md Hasibul Husain Hisham, Francisco Grisanti, James F Martin, Atif Rahman, Md Abul Hassan Samee

Faculty, Staff and Students Publications

A critical challenge of single-cell spatial transcriptomics (sc-ST) technologies is their panel size. Being based on fluorescence in situ hybridization, they are typically limited to panels of about a thousand genes. This constrains researchers to build panels from only the marker genes of different cell types and forgo other genes of interest, e.g., genes encoding ligand-receptor complexes or those in specific pathways. We propose scGIST, a constrained feature selection tool that designs sc-ST panels prioritizing user-specified genes without compromising cell type detection accuracy. We demonstrate scGIST's efficacy in diverse use cases, highlighting it as a valuable addition to sc-ST's algorithmic …

Evidence Review And Considerations For Use Of First Line Genome Sequencing To Diagnose Rare Genetic Disorders, Kristen M Wigby, Deanna Brockman, Gregory Costain, Caitlin Hale, Stacie L Taylor, John Belmont, David Bick, David Dimmock, Susan Fernbach, John Greally, Vaidehi Jobanputra, Shashikant Kulkarni, Elizabeth Spiteri, Ryan J Taft

Center for Medical Ethics and Health Policy Staff Publications

Early use of genome sequencing (GS) in the diagnostic odyssey can reduce suffering and improve care, but questions remain about which patient populations are most amenable to GS as a first-line diagnostic test. To address this, the Medical Genome Initiative conducted a literature review to identify appropriate clinical indications for GS. Studies published from January 2011 to August 2022 that reported on the diagnostic yield (DY) or clinical utility of GS were included. An exploratory meta-analysis using a random effects model evaluated DY based on cohort size and diagnosed cases per cohort. Seventy-one studies met inclusion criteria, comprising over 13,000 …

Medical Monitoring Working Group Focused Call Summary Amendment, Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

2024 Draft Final Unreclaimed Sites Quality Assurance Project Plan (Qapp), Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Final 2024 Residential Metals Abatement Program (Rmap) Whitehall Borrow Submittal #2, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Polyaminer-Bulk Is A Deep Learning-Based Algorithm That Decodes Alternative Polyadenylation Dynamics From Bulk Rna-Seq Data, Venkata Soumith Jonnakuti, Eric J Wagner, Mirjana Maletić-Savatić, Zhandong Liu, Hari Krishna Yalamanchili

Faculty, Staff and Students Publications

Alternative polyadenylation (APA) is a key post-transcriptional regulatory mechanism; yet, its regulation and impact on human diseases remain understudied. Existing bulk RNA sequencing (RNA-seq)-based APA methods predominantly rely on predefined annotations, severely impacting their ability to decode novel tissue- and disease-specific APA changes. Furthermore, they only account for the most proximal and distal cleavage and polyadenylation sites (C/PASs). Deconvoluting overlapping C/PASs and the inherent noisy 3' UTR coverage in bulk RNA-seq data pose additional challenges. To overcome these limitations, we introduce PolyAMiner-Bulk, an attention-based deep learning algorithm that accurately recapitulates C/PAS sequence grammar, resolves overlapping C/PASs, captures non-proximal-to-distal APA changes, …

Aging Impairs The Osteocytic Regulation Of Collagen Integrity And Bone Quality, Charles A Schurman, Serra Kaya, Neha Dole, Nadja M Maldonado Luna, Natalia Castillo, Ryan Potter, Jacob P Rose, Joanna Bons, Christina D King, Jordan B Burton, Birgit Schilling, Simon Melov, Simon Tang, Eric Schaible, Tamara Alliston

2020-Current year OA Pubs

Poor bone quality is a major factor in skeletal fragility in elderly individuals. The molecular mechanisms that establish and maintain bone quality, independent of bone mass, are unknown but are thought to be primarily determined by osteocytes. We hypothesize that the age-related decline in bone quality results from the suppression of osteocyte perilacunar/canalicular remodeling (PLR), which maintains bone material properties. We examined bones from young and aged mice with osteocyte-intrinsic repression of TGFβ signaling (TβRII

A Simple Method For Rapid Cloning Of Complete Herpesvirus Genomes, Jan Knickmann, Laura Staliunaite, Olha Puhach, Eleonore Ostermann, Thomas Günther, Jenna Nichols, Michael A. Jarvis, Sebastian Voigt, Adam Grundhoff, Andrew J. Davison, Wolfram Brune

School of Biomedical Sciences

Herpesviruses are large DNA viruses and include important human and veterinary pathogens. Their genomes can be cloned as bacterial artificial chromosomes (BACs) and genetically engineered in Escherichia coli using BAC recombineering methods. While the recombineering methods are efficient, the initial BAC-cloning step remains laborious. To overcome this limitation, we have developed a simple, rapid, and efficient BAC-cloning method based on single-step transformation-associated recombination (STAR) in Saccharomyces cerevisiae. The linear viral genome is directly integrated into a vector comprising a yeast centromeric plasmid and a BAC replicon. Following transfer into E. coli, the viral genome can be modified using standard BAC …

Investigating Post-Traumatic Syringomyelia And Local Fluid Osmoregulation Via A Rat Model, Dipak D Pukale, Kayla Adkins-Travis, Siddhartha R Aryal, Leah P Shriver, Gary J Patti, Nic D Leipzig

2020-Current year OA Pubs

BACKGROUND: Syringomyelia (SM) is characterized by the development of fluid-filled cavities, referred to as syrinxes, within the spinal cord tissue. The molecular etiology of SM post-spinal cord injury (SCI) is not well understood and only invasive surgical based treatments are available to treat SM clinically. This study builds upon our previous omics studies and in vitro cellular investigations to further understand local fluid osmoregulation in post-traumatic SM (PTSM) to highlight important pathways for future molecular interventions.

METHODS: A rat PTSM model consisting of a laminectomy at the C7 to T1 level followed by a parenchymal injection of 2 μL quisqualic …

Sex And Gender In Ageing And Longevity: Highlights From An International Course, Giuseppina Candore, Giulia Accardi, Anna Aiello, Giovannella Baggio, Tiziana Bellini, Vittorio Calabrese, Anna Paola Carreca, Ignazio Carreca, Anna Masucci, Monica Cattaneo, Serena Dato, Danilo Di Bona, Luca Fabris, Caterina Gambino, Gabriele Di Lorenzo, Claudio Franceschi, Mattia Emanuela Ligotti, Maria Cristina Manfrinato, Annibale Alessandro Puca, Martina Tamburello, Roberta Vassallo, Calogero Caruso

Translational Medicine @ UniSa

Gender medicine is a multidisciplinary science and represents an important perspective for pathophysiological and clinical studies in the third millennium. Here, it is provided an overview of the topics discussed in a recent course on the Role of Sex and Gender in Ageing and Longevity. The paper highlights three themes discussed in the course, i.e., the interaction of gender/sex with, i) the pathophysiology of age-related diseases; ii), the role of genetics and epigenetics in ageing and longevity and, iii) the immune responses of older people to pathogens, vaccines, autoantigens, and allergens. Although largely unexplored, sex and gender are modulators …

Effect Of Education Program On Mothers' Knowledge About Breastfeeding In Al-Hodieadh Governorate-Yemen 2021, Haddad Salim Alhebshi, Noman Ahmed Alhatemi, Laith Mahmoud Alosoufe, Muhannad Abdulkawi Al-Fakih

Hadhramout University Journal of Natural & Applied Sciences

Breastfeeding is an essential and important for public health and growth newborn. It is considered as the appropriate feeding method to provide necessary nutrition for the newborn. The study aimed to increase mothers' knowledge about breastfeeding. Methods: The quasi-experimental research design was utilized in this study. The study revealed that there is an effect of the education program on mothers' knowledge toward breastfeeding. The total score of mothers' knowledge about breastfeeding showed that; 8.1% of mothers had good knowledge in pretest and their knowledge improved after being implemented to be 76.3% in posttest, and 56.3% in follow up test. The …

Shepherds Leading The Flocks: A Qualitative Phenomenological Study Of Church Leaders Managing Church Membership During The Covid-19 Pandemic, Rbetrice Phillips

Dissertations

Purpose: The purpose of this phenomenological study was to identify and describe experiences of church leaders adapting the Scarneo (2019) intrapersonal and interpersonal socioecological influences while managing membership during the COVID-19 Pandemic.

Methodology: This study used a qualitative phenomenological approach with a socioecological justification with open-ended interview questions investigating the lived experiences of the participants. This study aimed to gather data from sample size 10 church leaders in Los Angeles County, California plus a snowball sampling of referred church leaders who managed during COVID-19. In this study, the collection of narratives through semi-structured interviews provided the data which was analyzed …

Dietary Adherence And Cognitive Performance In Older Adults By Nativity Status: Results From The National Health And Nutrition Examination Survey (Nhanes), 2011–2014, Md Towfiqul Alam, Elizabeth Vásquez, Jennifer L. Etnier, Sandra Echeverria

Department of Health Sciences - Faculty Scholarship

Although adherence to dietary guidelines is associated with better cognitive performance, there may be differences by nativity status. This study aimed to investigate the association between adherence to the healthy eating index (HEI) and cognitive performance (CP) among United States (US)-born and foreign-born older adults (60+ years). Data were obtained from the 2011–2014 NHANES (n = 3065). Dietary adherence was assessed with HEI quintiles. CP (adequate vs. low) was examined using word listing (CERAD), animal naming (AFT), and the digit symbol substitution test (DSST). Weighted multivariable logistic regressions were used to examine associations. The US-born participants in higher dietary quintiles …

Er Or Urgent Care? Which Is Best For Your Child?, Meggie Mckay, Jennifer Johnson

Parent-ish Podcast

The inevitable happened – a broken bone, illness or another ailment for your child. And it happened while your doctor’s office is closed. So, the dilemma every parent faces …do you go to the Emergency Room (ER) or Urgent Care?

Click here to stream the podcast episode.