Digital Commons Network^™

Anesthetic Management Of Unanticipated Subglottic Stenosis In A Patient Undergoing Coronary Artery Bypass Graft Surgery, Claudia Wei, Dominique Wreh, Jacy Gressen, Anvinh Nguyen

Faculty, Staff and Students Publications

A 62-year-old female presented for a scheduled coronary artery bypass graft (CABG) and was found to have an unexpected subglottic stenosis during routine intubation. The case was aborted and six days later, the patient underwent lasering of the stenotic subglottic region and airway balloon dilation. In this case report, causes of subglottic stenosis and surgical/anesthetic management of the condition are discussed. The management of subglottic stenosis in this patient was complicated by concurrent severe coronary artery disease (CAD) involving the left main coronary artery and timing of airway surgery relative to interventions for her CAD. In situations of undiagnosed subglottic …

Ketamine For Pain In Sickle Cell Disease Reduces Opioid Usage, Christina O Onyebuchi, Corrie E Chumpitazi, Jennifer L Placencia, Andrea N Jackson, Jennifer L Jones, Laura Torres, Venée N Tubman

Faculty, Staff and Students Publications

CONTEXT: Pain attributable to sickle cell disease (SCD) is often unpredictable, recurrent, and requires complex treatments. Subanesthetic ketamine infusion has been studied in other diseases and disorders, but there is still limited data on its efficacy in pain management for SCD.

OBJECTIVES: The primary objective is to determine if subanesthetic ketamine infusion reduces pain scores and opioid requirements in hospitalized pediatric patients with SCD.

RESULTS: Forty-six admissions among 22 patients between February 2018 and December 2019 were analyzed. We observed decrease in pain scores within 24 hours of ketamine initiation in 34 of 46 admissions (mean pain score per patient …

Limonene Enantiomeric Ratios From Anthropogenic And Biogenic Emission Sources, Shan Gu, Wentai Luo, Avisa Charmchi, Kevin J. Mcwhirter, Todd Rosenstiel, James F. Pankow

Chemistry Faculty Publications and Presentations

Emissions from volatile chemical products (VCPs) have been identified as contributors to air quality degradation in urban areas. Limonene can be a tracer compound for VCPs containing fragrances in densely populated regions, but limonene is also emitted from conifers that are planted in urban areas. This creates challenges for using limonene to estimate VCP emissions. In this study, the −/+ enantiomeric ratios of limonene from VCP and conifer emission sources were quantified to evaluate if this measurement could be used to aid in source apportionment and emission inventory development. Samples were analyzed using a gas chromatograph equipped with a chiral …

Preoperative Hemodynamics Impact The Benefit Of Fenestration On Fontan Postoperative Length Of Stay, Garick D Hill, Danny T Y Wu, M Eric Ferguson, Saul Flores, Salil Ginde, Kevin D Hill, Michael Johansen, Jane W Newburger, Zhiqian Gao, James F Cnota

Faculty, Staff and Students Publications

BACKGROUND: Utilization of Fontan fenestration varies considerably by center.

OBJECTIVES: Using a multicenter Pediatric Heart Network dataset linking surgical and preoperative hemodynamic variables, the authors evaluated factors associated with use of Fontan fenestration and the impact of fenestration on post-Fontan length of stay (LOS).

METHODS: Patients 2 to 6 years old at Fontan surgery from 2010 to 2020 with catheterizationeffect.

RESULTS: Fenestration was used in 465 of 702 patients (66.2%). Placement of a fenestration was associated with center (range 27%-93% use,

CONCLUSIONS: There is wide center variability in use of Fontan fenestration that is not explained by preoperative hemodynamics. Fenestration …

Incongruity Between T Cell Receptor Recognition Of Breast Cancer Hotspot Mutations Esr1 Y537s And D538g Following Exogenous Peptide Loading Versus Endogenous Antigen Processing, Paul Shafer, Wingchi K Leung, Mae Woods, Jong Min Choi, Carlos M Rodriguez-Plata, Arushana Maknojia, Andres Mosquera, Lauren K Somes, Jarrett Joubert, Anthony Manliguez, Rashi Ranjan, Bryan Burt, Hyun-Sung Lee, Bing Zhang, Suzanne Fuqua, Cliona Rooney, Ann M Leen, Valentina Hoyos

Faculty, Staff and Students Publications

T cell receptor engineered T cell (TCR T) therapies have shown recent efficacy against certain types of solid metastatic cancers. However, to extend TCR T therapies to treat more patients across additional cancer types, new TCRs recognizing cancer-specific antigen targets are needed. Driver mutations in AKT1, ESR1, PIK3CA, and TP53 are common in patients with metastatic breast cancer (MBC) and if immunogenic could serve as ideal tumor-specific targets for TCR T therapy to treat this disease. Through IFN-γ ELISpot screening of in vitro expanded neopeptide-stimulated T cell lines from healthy donors and MBC patients, we identified reactivity towards 11 of …

Tuberculosis In People Of Ukrainian Origin In The European Union And The European Economic Area, 2019 To 2022, Krista Stoycheva, Veronica Cristea, Csaba Ködmön, Senia Rosales-Klintz, Dominik Zenner, Anca Vasiliu, Marieke Van Der Werf, Christoph Lange

Faculty, Staff and Students Publications

Approximately five million Ukrainians were displaced to the EU/EEA following the Russian invasion of Ukraine. While tuberculosis (TB) notification rates per 100,000 Ukrainians in the EU/EEA remained stable, the number of notified TB cases in Ukrainians increased almost fourfold (mean 2019-2021: 201; 2022: 780). In 2022, 71% cases were notified in three countries, and almost 20% of drug-resistant TB cases were of Ukrainian origin. Targeted healthcare services for Ukrainians are vital for early diagnosis and treatment, and preventing transmission.

Demystifying The Link Between Social Media Addiction And Sharing Without Verification: The Role Of Absentmindedness And Wellbeing, Murad Moqbel, Khaled A. Alshare, Michael A. Erskine

Information Systems Faculty Publications

We use salience and dual-system theories as the lens to investigate how (via which intervening mechanism) and when (under what condition(s)) social media addiction impacts unverified information sharing. Based on results from analyzing data from 234 social media users, we found that social media addiction augments unverified information sharing, and that absentmindedness partially mediates this relationship. Furthermore, we establish that wellbeing status buffers the harmful impact of social media addiction on unverified information sharing and absentmindedness.

Mitochondrial Involvement In Sarcopenia, Charles Affourtit, Jane E. Carré

School of Biomedical Sciences

Sarcopenia lowers the quality-of-life for millions of people across the world, as accelerated loss of skeletal muscle mass and function contributes to both age- and disease-related frailty. Physical activity remains the only proven therapy for sarcopenia to date, but alternatives are much sought after to manage this progressive muscle disorder in individuals who are unable to exercise. Mitochondria have been widely implicated in the etiology of sarcopenia and are increasingly suggested as attractive therapeutic targets to help restore the perturbed balance between protein synthesis and breakdown that underpins skeletal muscle atrophy. Reviewing current literature, we note that mitochondrial bioenergetic changes …

Natural Gas Induced Vegetation Stress Identification And Discrimination From Hyperspectral Imaging For Pipeline Leakage Detection, Pengfei Ma, Ying Zhuo, Genda Chen, Joel G. Burken

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Remote Sensing Detection of Natural Gas Leaks Remains Challenging When using Ground Vegetation Stress to Detect Underground Pipeline Leaks. Other Natural Stressors May Co-Present and Complicate Gas Leak Detection. This Study Explores the Feasibility of Identifying and Distinguishing Gas-Induced Stress from Other Natural Stresses by Analyzing the Hyperspectral Reflectance of Vegetation. the Effectiveness of This Discrimination is Assessed Across Three Distinct Spectral Ranges (VNIR, SWIR, and Full Spectra). Greenhouse Experiments Subjected Three Plant Species to Controlled Environmental Stressors, Including Gas Leakage, Salinity Impact, Heavy-Metal Contamination, and Drought Exposure. Spectral Curves Obtained from the Experiments Underwent Preprocessing Techniques Such as Standard …

Failure Rate Of Anti-Tumor Necrosis Factor Α Biologics In Very Early Onset Inflammatory Bowel Disease, Ashleigh Watson, Lina B Karam, Richard Kellermayer

Faculty, Staff and Students Publications

No abstract provided.

Achieving Equity In Child And Adolescent Mental Health By Addressing Racism Through Prevention Science, Nada M Goodrum, Daniel K Cooper, Sarah Edmunds, Guillermo M Wippold, Jessica Bradshaw, Julie K Nguyen, Norweeta Milburn, Funlola Are

Faculty, Staff and Student Publications

Prevention science is a multidisciplinary field dedicated to promoting public health and reducing early risk factors that lead to negative health outcomes. It has been used to successfully improve child and family mental health and well-being, including for families affected by adversity. Despite advances in prevention efforts, major public health inequities remain for Black, Indigenous, and other People of Color (BIPOC) children and families, in part because of equity-implicit "one-size-fits-all" approaches that do not directly address racism which in part underlies the very health concerns these efforts aim to prevent. Structural racism not only introduces additional risk for negative health …

Discovering The Regulators Of Heat Stress Tolerance In Ziziphus Nummularia (Burm.F) Wight And Walk.-Arn, Kishor Prabhakar Panzade, Kishor U Tribhuvan, Deepak V Pawar, Rahul Singh Jasrotia, Kishor Gaikwad, Monika Dalal, Ranjeet Ranjan Kumar, Madan Pal Singh, Om Prakash Awasthi, Jasdeep Chatrath Padaria

Faculty, Staff and Student Publications

Ziziphus nummularia an elite heat-stress tolerant shrub, grows in arid regions of desert. However, its molecular mechanism responsible for heat stress tolerance is unexplored. Therefore, we analysed whole transcriptome of Jaisalmer (heat tolerant) and Godhra (heat sensitive) genotypes of Z. nummularia to understand its molecular mechanism responsible for heat stress tolerance. De novo assembly of 16,22,25,052 clean reads yielded 276,029 transcripts. A total of 208,506 unigenes were identified which contains 4290 and 1043 differentially expressed genes (DEG) in TGO (treated Godhra at 42 °C) vs. CGO (control Godhra) and TJR (treated Jaisalmer at 42 °C) vs. CJR (control Jaisalmer), respectively. …

A Comparative Study Of The Business Models Used In The U.S. And Saudi Arabia Firms In Relation To Venture Capital, Abdulmajeed Ali Alkthiri

Theses and Dissertations

While venture capital (VC) has expanded globally, challenges in comparing international VC strategies remain due to data limitations and theoretical variability. This study contrasts VC investment philosophies, support mechanisms, and exit strategies in mature US firms with those in developing Saudi Arabia from 2016-2021. Analyzing fundraising, sector focus, deal volume, and exit outcomes, it finds significant differences attributed to ecosystem maturity, with some convergence in contracting and mentoring. The findings highlight the need for US VC models to adapt strategically in Islamic markets, suggesting regulatory reforms for market development. This research provides guidelines for effective cross-border VC strategy adaptation.

Renal Response Outcomes Of The Eurolupus And National Institutes Of Health Cyclophosphamide Dosing Regimens In Childhood-Onset Proliferative Lupus Nephritis, Christine S Wang, Rebecca E Sadun, Wenru Zhou, Kristen R Miller, Laura Pyle, Stacey P Ardoin, Christine Bacha, Emily Hause, Joyce Hui-Yuen, Nicole Ling, Maria Pereira, Meredith Riebschleger, Kelly Rouster-Stevens, Aliese Sarkissian, Julia Shalen, William Soulsby, Marinka Twilt, Eveline Y Wu, Laura B Lewandowski, Scott E Wenderfer, Jennifer C Cooper

Faculty, Staff and Students Publications

Objective: We compared clinical characteristics and renal response in patients with childhood-onset proliferative lupus nephritis (LN) treated with the EuroLupus versus National Institutes of Health (NIH) cyclophosphamide (CYC) regimen.

Methods: A retrospective cohort study was conducted at 11 pediatric centers in North America that reported using both CYC regimens. Data were extracted from the electronic medical record at baseline and 3, 6, and 12 months after treatment initiation with CYC. To evaluate the adjusted association between CYC regimen (EuroLupus vs NIH) and renal response over time, generalized estimating equations with a logit link were used. An interaction between time and …

Body Size And Risk Of Colorectal Cancer Molecular Defined Subtypes And Pathways: Mendelian Randomization Analyses, Nikos Papadimitriou, Yin Cao, Et Al.

2020-Current year OA Pubs

BACKGROUND: Obesity has been positively associated with most molecular subtypes of colorectal cancer (CRC); however, the magnitude and the causality of these associations is uncertain.

METHODS: We used Mendelian randomization (MR) to examine potential causal relationships between body size traits (body mass index [BMI], waist circumference, and body fat percentage) with risks of Jass classification types and individual subtypes of CRC (microsatellite instability [MSI] status, CpG island methylator phenotype [CIMP] status, BRAF and KRAS mutations). Summary data on tumour markers were obtained from two genetic consortia (CCFR, GECCO).

FINDINGS: A 1-standard deviation (SD:5.1 kg/m

INTERPRETATION: Body size was more strongly …

Structural Basis Of A Transcription Pre-Initiation Complex On A Divergent Promoter, Tiago R D Costa, Jonasz B Patkowski, Kévin Macé, Peter J Christie, Gabriel Waksman

Faculty, Staff and Student Publications

Considerable progress has been made in recent years in the structural and molecular biology of type IV secretion systems in Gram-negative bacteria. The latest advances have substantially improved our understanding of the mechanisms underlying the recruitment and delivery of DNA and protein substrates to the extracellular environment or target cells. In this Review, we aim to summarize these exciting structural and molecular biology findings and to discuss their functional implications for substrate recognition, recruitment and translocation, as well as the biogenesis of extracellular pili. We also describe adaptations necessary for deploying a breadth of processes, such as bacterial survival, host–pathogen …

Emotion Regulation And Cheap Talk As Signaling Strategies: Evidence From Crowdfunding For Ukraine, Alexandra Fischmann, Larry Zhiming Xu

College of Communication Faculty Research and Publications

Crowdfunding, as enabled by donors worldwide, has played a critical role in financially supporting military forces, NGOs, humanitarian groups, and civilians in the Ukraine-Russia Crisis. This global mobilization underscores the evolving dynamics in the modern geopolitical landscape, emphasizing the transformative power of online collective action in fundraising and organizing. This study draws on signaling theory to pinpoint success indicators in crowdfunding for Ukraine projects on GoFundMe. Recognized as an effective explanatory framework, signaling theory is used as it bridges fundraising tactics with donation behavior in crowdfunding contexts. Particularly, the research examines how emotions and semantics contributed to (or hindered) fundraising …

Declining Prevalence Of Trichomonas Vaginalis Diagnosed By Wet Mount In A Cohort Of U.S. Women With And Without Hiv, Elizabeth M Daubert, Jodie Dionne, Jessica Atrio, Andrea K Knittel, Seble G Kassaye, Dominika Seidman, Amanda Long, Susan Brockmann, Igho Ofotokun, Margaret A Fischl, L Stewart Massad, Kathleen M Weber

2020-Current year OA Pubs

No abstract provided.

Gene-Based Association Study Of Rare Variants In Children Of Diverse Ancestries Implicates Tnfrsf21 In The Development Of Allergic Asthma, Selene Clay, Katherine Rivera-Spoljaric, Et Al.

2020-Current year OA Pubs

BACKGROUND: Most genetic studies of asthma and allergy have focused on common variation in individuals primarily of European ancestry. Studying the role of rare variation in quantitative phenotypes and in asthma phenotypes in populations of diverse ancestries can provide additional, important insights into the development of these traits.

OBJECTIVE: We sought to examine the contribution of rare variants to different asthma- or allergy-associated quantitative traits in children with diverse ancestries and explore their role in asthma phenotypes.

METHODS: We examined whole-genome sequencing data from children participants in longitudinal studies of asthma (n = 1035; parent-identified as 67% Black and 25% …

Predictors Of Digital Amputation In Diabetic Patients With Surgically Treated Finger Infections, Ella Gibson, Carrie R Bettlach, Emma Payne, John Daines, Linh Vuong, Corinne Merrill, Ida K Fox, Mitchell A Pet

2020-Current year OA Pubs

BACKGROUND: Diabetes is a well-established risk factor for severe digital infection, and patients are more likely to require digital amputation for adequate source control. This study aims to identify factors predictive of digital amputation compared with preservation in patients with diabetes who present with surgically treated finger infections.

METHODS: Current Procedural Terminology (CPT) and International Classification of Diseases Versions 9 and 10 (ICD-9/10) databases from a single academic medical center were queried to identify patients with type 1 or type 2 diabetes mellitus who underwent surgical treatment in the operating room for treatment of a digital infection from 2010 to …

Impact Of Diagnostic Stewardship Interventions In The Collection Process Of Fungal Blood Cultures, Miguel A Chavez, Satish Munigala, Carey-Ann D Burnham, Melanie L Yarbrough, Crystal Squires, Josephine Fox, Heather Gasama, Kevin Hsueh, David K Warren

2020-Current year OA Pubs

We implemented 2 interventions to improve utilization and contamination at our institution: kits to improve appropriate sample collection and an electronic order alert displaying appropriate indications of fungal blood cultures. An electronic order alert when ordering fungal blood cultures was associated with decreased utilization without decrease in positivity rate.

Hypoimmune Induced Pluripotent Stem Cells Survive Long Term In Fully Immunocompetent, Allogeneic Rhesus Macaques, Xiaomeng Hu, Nathaniel J Hogrebe, Et Al.

2020-Current year OA Pubs

Genetic engineering of allogeneic cell therapeutics that fully prevents rejection by a recipient's immune system would abolish the requirement for immunosuppressive drugs or encapsulation and support large-scale manufacturing of off-the-shelf cell products. Previously, we generated mouse and human hypoimmune pluripotent (HIP) stem cells by depleting HLA class I and II molecules and overexpressing CD47 (B2M

Distributed Feature Representations Of Natural Stimuli Across Parallel Retinal Pathways, Jen-Chun Hsiang, Ning Shen, Florentina Soto, Daniel Kerschensteiner

2020-Current year OA Pubs

How sensory systems extract salient features from natural environments and organize them across neural pathways is unclear. Combining single-cell and population two-photon calcium imaging in mice, we discover that retinal ON bipolar cells (second-order neurons of the visual system) are divided into two blocks of four types. The two blocks distribute temporal and spatial information encoding, respectively. ON bipolar cell axons co-stratify within each block, but separate laminarly between them (upper block: diverse temporal, uniform spatial tuning; lower block: diverse spatial, uniform temporal tuning). ON bipolar cells extract temporal and spatial features similarly from artificial and naturalistic stimuli. In addition, …

Gene Dosage Of Independent Dynein Arm Motor Preassembly Factors Influences Cilia Assembly In Chlamydomonas Reinhardtii, Gervette M. Penny, Susan K. Dutcher

2020-Current year OA Pubs

Motile cilia assembly utilizes over 800 structural and cytoplasmic proteins. Variants in approximately 58 genes cause primary ciliary dyskinesia (PCD) in humans, including the dynein arm (pre)assembly factor (DNAAF) gene DNAAF4. In humans, outer dynein arms (ODAs) and inner dynein arms (IDAs) fail to assemble motile cilia when DNAAF4 function is disrupted. In Chlamydomonas reinhardtii, a ciliated unicellular alga, the DNAAF4 ortholog is called PF23. The pf23-1 mutant assembles short cilia and lacks IDAs, but partially retains ODAs. The cilia of a new null allele (pf23-4) completely lack ODAs and IDAs and are even shorter than cilia from pf23-1. In …

Hepsin Promotes Breast Tumor Growth Signaling Via The Tgfβ-Egfr Axis, Denis Belitškin, Pauliina Munne, Shishir M Pant, Johanna M Anttila, Ilida Suleymanova, Kati Belitškina, Daniel Kirchhofer, James Janetka, Taivo Käsper, Sami Jalil, Jeroen Pouwels, Topi A Tervonen, Juha Klefström

2020-Current year OA Pubs

Hepsin, a type II transmembrane serine protease, is commonly overexpressed in prostate and breast cancer. The hepsin protein is stabilized by the Ras-MAPK pathway, and, downstream, this protease regulates the degradation of extracellular matrix components and activates growth factor pathways, such as the hepatocyte growth factor (HGF) and transforming growth factor beta (TGFβ) pathway. However, how exactly active hepsin promotes cell proliferation machinery to sustain tumor growth is not fully understood. Here, we show that genetic deletion of the gene encoding hepsin (Hpn) in a WAP-Myc model of aggressive MYC-driven breast cancer inhibits tumor growth in the primary syngrafted sites …

Clinical Characteristics And Treatment Exposure Of Patients With Marked Treatment-Resistant Unipolar Major Depressive Disorder: A Recover Trial Report, Charles R Conway, Christopher L Kriedt, Et Al.

2020-Current year OA Pubs

BACKGROUND: RECOVER is a randomized sham-controlled trial of vagus nerve stimulation and the largest such trial conducted with a psychiatric neuromodulation intervention.

OBJECTIVE: To describe pre-implantation baseline clinical characteristics and treatment history of patients with unipolar, major depressive disorder (MDD), overall and as a function of exposure to interventional psychiatric treatments (INTs), including electroconvulsive therapy, transcranial magnetic stimulation, and esketamine.

METHODS: Medical, psychiatric, and treatment records were reviewed by study investigators and an independent Study Eligibility Committee prior to study qualification. Clinical characteristics and treatment history (using Antidepressant Treatment History [Short] Form) were compared in those qualified (N = 493) …

Decreased Sarcoplasmic Reticulum Phospholipids In Human Skeletal Muscle Are Associated With Metabolic Syndrome, Samantha E Adamson, Sangeeta Adak, Max C Petersen, Dustin Higgins, Larry D Spears, Rong Mei Zhang, Andrea Cedeno, Alexis Mckee, Aswathi Kumar, Sudhir Singh, Fong-Fu Hsu, Janet B Mcgill, Clay F Semenkovich

2020-Current year OA Pubs

Metabolic syndrome affects more than one in three adults and is associated with increased risk of diabetes, cardiovascular disease, and all-cause mortality. Muscle insulin resistance is a major contributor to the development of the metabolic syndrome. Studies in mice have linked skeletal muscle sarcoplasmic reticulum (SR) phospholipid composition to sarcoplasmic/endoplasmic reticulum Ca

Comparing Bladder Neck Contracture Rate Between Robotic Intracorporeal And Extracorporeal Neobladder Construction, Rohit Bhatt, Dylan J. Mittauer, Joel M. Vetter, Nimrod S. Barashi, Riley Mcginnis, Kenneth G. Sands, Alexander K. Chow, Eric H. Kim

2020-Current year OA Pubs

Robot-assisted radical cystectomy (RARC) has become more accessible to surgeons worldwide, and descriptions of intracorporeal urinary diversion techniques, such as orthotopic neobladder construction, have increased. In this study, we aim to compare the rate of bladder neck contracture (BNC) formation between RARC and two different urinary diversion techniques. We retrospectively reviewed our institutional database for patients with bladder cancer who underwent RARC with intracorporeal neobladder (ICNB) construction (n = 11) or extracorporeal neobladder (ECNB) construction (n = 11) between 2012 and 2020. BNC was defined by the need for an additional surgical procedure (e.g., dilatation, urethrotomy). Patients who underwent RARC …

Altered Larval Activation Response Associated With Multidrug Resistance In The Canine Hookworm Ancylostoma Caninum, Elise L Mckean, Emilia Grill, Young-Jun Choi, Makedonka Mitreva, Damien M O'Halloran, John M Hawdon

2020-Current year OA Pubs

Parasitic gastrointestinal nematodes pose significant health risks to humans, livestock, and companion animals, and their control relies heavily on the use of anthelmintic drugs. Overuse of these drugs has led to the emergence of resistant nematode populations. Herein, a naturally occurring isolate (referred to as BCR) of the dog hookworm,

Genome And Clonal Hematopoiesis Stability Contrasts With Immune, Cfdna, Mitochondrial, And Telomere Length Changes During Short Duration Spaceflight, J Sebastian Garcia-Medina, Irenaeus Chan, Carlos Cruchaga, Kelly Bolton, Et Al.

2020-Current year OA Pubs

BACKGROUND: The Inspiration4 (I4) mission, the first all-civilian orbital flight mission, investigated the physiological effects of short-duration spaceflight through a multi-omic approach. Despite advances, there remains much to learn about human adaptation to spaceflight's unique challenges, including microgravity, immune system perturbations, and radiation exposure.

METHODS: To provide a detailed genetics analysis of the mission, we collected dried blood spots pre-, during, and post-flight for DNA extraction. Telomere length was measured by quantitative PCR, while whole genome and cfDNA sequencing provided insight into genomic stability and immune adaptations. A robust bioinformatic pipeline was used for data analysis, including variant calling to …