Digital Commons Network^™

Kibra Anchoring The Action Of Pkmζ Maintains The Persistence Of Memory, Panayiotis Tsokas, Changchi Hsieh, Rafael E Flores-Obando, Matteo Bernabo, Andrew Tcherepanov, A Iván Hernández, Christian Thomas, Peter J Bergold, James E Cottrell, Joachim Kremerskothen, Harel Z Shouval, Karim Nader, André A Fenton, Todd C Sacktor

Faculty, Staff and Student Publications

How can short-lived molecules selectively maintain the potentiation of activated synapses to sustain long-term memory? Here, we find kidney and brain expressed adaptor protein (KIBRA), a postsynaptic scaffolding protein genetically linked to human memory performance, complexes with protein kinase Mzeta (PKMζ), anchoring the kinase's potentiating action to maintain late-phase long-term potentiation (late-LTP) at activated synapses. Two structurally distinct antagonists of KIBRA-PKMζ dimerization disrupt established late-LTP and long-term spatial memory, yet neither measurably affects basal synaptic transmission. Neither antagonist affects PKMζ-independent LTP or memory that are maintained by compensating PKCs in ζ-knockout mice; thus, both agents require PKMζ for their effect. …

The Application Of Multi-Isotope Analysis To Assist In Geoprofiling Unidentified Decedents Within Florida, Liotta D. Noche-Dowdy

USF Tampa Graduate Theses and Dissertations

The biochemistry of human tissues can provide information for geoprofiling (geographic birthplace or migration patterns) of an unidentified decedent. Utilizing isotope analyses (both stable and radiogenic) from teeth, bone, nails, and/or hair reveals specific isotopic information that correlates to diverse geographic regions reflecting an individual’s birthplace and migration patterns. Isotope analysis of enamel and bone has been employed in bioarchaeology for decades to reconstruct the diet, health, and migration patterns of past populations. Isotope ratios measured from enamel can then be compared to isotope ratios measured from bone of the individual to track patterns of migration. The enamel from the …

Integrating Location Data Into Analytics: Methods, Applications, And Fairness In Public Healthcare, Arindam Ray

USF Tampa Graduate Theses and Dissertations

This dissertation explores the application of location data in healthcare management, focusing on epidemic spread forecasts and fairness in healthcare access. It demonstrates the power of location data in understanding and forecasting epidemic spread with two examples, using mobility metrics derived from the location data. The second example also considers the effect of social vulnerability along with population mobility and extends a popular deep-learning algorithm to accept static anddynamic data. The dissertation also proposes health equity measures by evaluating the alignment between healthcare facility locations and people’s mobility patterns, considering demographic factors. This research highlights the valuable insight location data …

Physiological And Circadian Outcomes Of Fkbp5 Imbalance: Insights From Two Models, Niat Tsegai Gebru

USF Tampa Graduate Theses and Dissertations

Circadian rhythms are crucial in regulating various physiological processes, including mood regulation, and disruptions in these rhythms have been linked to several neuropsychiatric disorders, such as depression, bipolar disorder, and schizophrenia. FKBP51, a protein involved in stress responses and glucocorticoid receptor function has emerged as a potential regulator of the circadian clock. FKBP51’s interaction with glucocorticoid receptors impacts the transcriptional regulation of circadian-related genes. In recent years, FKBP51 has been associated with genetic and epigenetic alterations in neuropsychiatric disorders, highlighting its potential as a target for intervention. The heterogeneity associated with neuropsychiatric disorders arises from a combination of genetic, environmental, …

The Role Of Pregnancy Intendedness In Shaping Contraceptive Practices And Family Planning Experiences: Evidence From Sub-Saharan Africa, Otobo I. Ujah

USF Tampa Graduate Theses and Dissertations

Background: Despite the associated adverse health and economic implications associated with unintended pregnancy, there remains a paucity of literature examining the effect of unintended pregnancy on postpartum contraceptive and reproductive behavior in many parts of Sub-Saharan Africa (SSA), including Nigeria.

Aims: The overall aim of this doctoral dissertation was twofold. First, to quantitatively examine whether and to what extent the intendedness of a woman’s most recent pregnancy is linked to subsequent contraceptive behaviors and family planning experiences. Secondly, to disentangle the influence of compositional and contextual level factors on these study outcomes. In the first paper, I investigated the extent …

Bet Inhibition Decreases Hmgcs2 And Sensitizes Resistant Pancreatic Tumors To Gemcitabine., Aubrey L. Miller, Samuel C. Fehling, Rebecca B. Vance, Dongquan Chen, Eric Josh Brown, M. Iqbal Hossain, Eric O. Heard, Shaida A. Andrabi, Hengbin Wang, Eddy S. Yang, Donald J. Buchsbaum, Robert C. A. M. Van Waardenburg, Susan L. Bellis, Karina J. Yoon

Markey Cancer Center Faculty Publications

Efforts to develop targetable molecular bases for drug resistance for pancreatic ductal adenocarcinoma (PDAC) have been equivocally successful. Using RNA-seq and ingenuity pathway analysis we identified that the superpathway of cholesterol biosynthesis is upregulated in gemcitabine resistant (gemR) tumors using a unique PDAC PDX model with resistance to gemcitabine acquired in vivo. Analysis of additional in vitro and in vivo gemR PDAC models showed that HMG-CoA synthase 2 (HMGCS2), an enzyme involved in cholesterol biosynthesis and rate limiting in ketogenesis, is overexpressed in these models. Mechanistic data demonstrate the novel findings that HMGCS2 contributes to gemR and confers metastatic properties …

Cutaneous Wound Healing And The Effects Of Cannabidiol, Pearl Shah, Kathryne Holmes, Fairouz Chibane, Phillip Wang, Pablo Chagas, Evila Salles, Melanie Jones, Patrick Palines, Mohamad Masoumy, Babak Baban, Jack Yu

School of Medicine Faculty Publications

Cutaneous wounds, both acute and chronic, begin with loss of the integrity, and thus barrier function, of the skin. Surgery and trauma produce acute wounds. There are 22 million surgical procedures per year in the United States alone, based on data from the American College of Surgeons, resulting in a prevalence of 6.67%. Acute traumatic wounds requiring repair total 8 million per year, 2.42% or 24.2 per 1000. The cost of wound care is increasing; it approached USD 100 billion for just Medicare in 2018. This burden for wound care will continue to rise with population aging, the increase in …

The Cns Relapse In T-Cell Lymphoma Index Predicts Cns Relapse In Patients With T- And Nk-Cell Lymphomas, Rahul S. Bhansali, Fredrik Ellin, Thomas Relander, Miao Cao, Wenrui Li, Qi Long, Nivetha Ganesan, Robert Stuver, Steven M. Horwitz, Kitsada Wudhikarn, Steven R. Hwang, N. Nora Bennani, Julio Chavez, Lubomir Sokol, Hayder Saeed, Frank Duan, Pierluigi Porcu, Priyanka Pullarkat, Neha Mehta-Shah, Jasmine M. Zain, Miguel Ruiz, Jonathan E. Brammer, Rishab Prakash, Swaminathan P. Iyer, Adam J. Olszewski, Ajay Major, Peter A. Riedell, Sonali M. Smith, Caroline Goldin, Bradley Haverkos, Bei Hu, Tony Z. Zhuang, Pamela B. Allen, Wael Toama, Murali Janakiram, Taylor R. Brooks, Deepa Jagadeesh, Nisha Hariharan, Aaron M. Goodman, Gabrielle Hartman, Paola Ghione, Fatima Fayyaz, Joanna M. Rhodes, Elise A. Chong, James N. Gerson, Daniel J. Landsburg, Sunita D. Nasta, Stephen J. Schuster, Jakub Svoboda, Mats Jerkeman, Stefan K. Barta

Department of Medical Oncology Faculty Papers

Little is known about risk factors for central nervous system (CNS) relapse in mature T-cell and natural killer cell neoplasms (MTNKNs). We aimed to describe the clinical epidemiology of CNS relapse in patients with MTNKN and developed the CNS relapse In T-cell lymphoma Index (CITI) to predict patients at the highest risk of CNS relapse. We reviewed data from 135 patients with MTNKN and CNS relapse from 19 North American institutions. After exclusion of leukemic and most cutaneous forms of MTNKNs, patients were pooled with non-CNS relapse control patients from a single institution to create a CNS relapse-enriched training set. …

Butte Mine Flooding Operable Unit Berkeley Pit And Discharge Pilot Project - May 2024 Monthly Discharge Monitoring Report, Mark Thompson, Dave Griffis

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

The Utilisation Of Rice Husk Ash Leachates For The Synthesis Of Eco-Friendly Geopolymers, Maria Kaka Etete Enoh, Oliver Ibor Inah

Platform: A Journal of Engineering (PAJE)

Geopolymers are inorganic polymers projected as feasible alternatives to Portland cement due to their lower CO2 emissions and high mechanical properties. In recent times, however, their impact on other environmental indices such as abiotic depletion, freshwater toxicity, marine ecotoxicity, terrestrial ecotoxicity, acidification, and human toxicity has become questionable, thus the need to investigate alternative eco-friendly precursors and activators. This work aims to manufacture an eco-efficient geopolymer by utilising biomass ash leachate as an alternative to the conventional alkali silicate solution. The leachate was obtained by soaking the rice husk ash (RHA) in a 10 M concentration of NaOH solution and …

The Discovery Of First Ret Protac With In Vivo Activity, Yafeng Wang

USF Tampa Graduate Theses and Dissertations

This report mainly discusses the development of RET Proteolysis targeting chimera PROTAC molecules based on Selpercatinib (LOXO-292) and Sulfono-γ-AApeptide PROTAC. A PROTAC molecule is a bifunctional molecule composed of two active domains and a linker. It works by inducing selective protein intracellular degradation. This technology was first discussed by Kathleen Sakamoto, Ray Deshaies and Craig Crews in 2001. Using various E3 Ligase, such as CRBN, pVHL, beta-TrCP1, Mdm2, DCAF15, DCAF16, RNF114, and c-IAP1, the PROTAC technology has been applied to several novel drug development,. Yale University licensed the PROTAC technology to Arvinas in 2013–14. For my research, Chapter one summarizes …

3d Organ-Scale Models Of Tumor Growth And Treatment, Rafael Ramon Bravo

USF Tampa Graduate Theses and Dissertations

To understand the dynamics of cancer, mathematical oncologists have developed models of tumor growth and treatment response. Some models are mechanistic and approach tumor growth at the cell-scale, focusing on the evolution of cancerous cells within the ecology of normal tissue, and are often simulated with agent-based modeling. Other models are more clinically motivated and model tumor growth operating at the organ-scale, using patient data to predict treatment response, and are often simulated with partial differential equations. We developed the Hybrid Automata Library which includes both agent-based modeling and partial differential equations for modeling at either of these scales. We …

Bayesian Analysis Of Hospital Service Quality: Pre- And Post-Covid, Ling Hu

USF Tampa Graduate Theses and Dissertations

The hospital sector is the largest revenue-generating industry in the United States, with a revenue of $1.4 trillion in 2022, accounting for 5.8% of the U.S. GDP. Its service quality profoundly impacts societal welfare, especially given the aging population reached 55.8 million in 2020 and is expected to reach 82 million by 2050. Therefore, multiple policies aim to incentivize sustained healthcare service quality improvements. As an integral component of the Hospital Value-Based Purchasing (HVBP) Program, the Hospital Consumer Assessment of Healthcare Providers and Systems (HCAHPS) survey, administrated by the Centers for Medicare and Medicaid Services (CMS), serves as a cornerstone …

The Influence Of Recycled Water On Microbial Source Tracking Techniques That Aim To Identify Untreated Sewage In Surface Waters, Aldo E. Lobos

USF Tampa Graduate Theses and Dissertations

Fecal pollution in recreational waters can introduce pathogens that cause increased human health risk, where exposure can occur during recreational activities such as swimming, diving, surfing, and fishing. Furthermore, human fecal pollution (i.e., sewage) can introduce nutrients and other harmful chemicals that may negatively affect the environment and aquatic species. Generally, sewage is considered the highest risk to human health compared to animal fecal pollution because it typically contains a high diversity of pathogens (including human specific viruses), antibiotic resistant bacteria and genes associated with antibiotic resistance. However, pathogens are difficult to detect due to their low concentrations in the …

Author Correction: Plasmonic Nano-Aperture Label-Free Imaging Of Single Small Extracellular Vesicles For Cancer Detection, Nareg Ohannesian, Mohammad Sadman Mallick, Jianzhong He, Yawei Qiao, Nan Li, Simona F Shaitelman, Chad Tang, Eileen H Shinn, Wayne L Hofstetter, Alexei Goltsov, Manal M Hassan, Kelly K Hunt, Steven H Lin, Wei-Chuan Shih

Faculty, Staff and Student Publications

No abstract provided.

Type I Conventional Dendritic Cells Facilitate Immunotherapy In Pancreatic Cancer, Krishnan K Mahadevan, Allison M Dyevoich, Yang Chen, Bingrui Li, Hikaru Sugimoto, Amari M Sockwell, Kathleen M Mcandrews, Lakshmi Kavitha Sthanam, Huamin Wang, Shabnam Shalapour, Stephanie S Watowich, Raghu Kalluri

Faculty, Staff and Student Publications

Inflammation and tissue damage associated with pancreatitis can precede or occur concurrently with pancreatic ductal adenocarcinoma (PDAC). We demonstrate that in PDAC coupled with pancreatitis (ptPDAC), antigen-presenting type I conventional dendritic cells (cDC1s) are specifically activated. Immune checkpoint blockade therapy (iCBT) leads to cytotoxic CD8+ T cell activation and elimination of ptPDAC with restoration of life span even upon PDAC rechallenge. Using PDAC antigen-loaded cDC1s as a vaccine, immunotherapy-resistant PDAC was rendered sensitive to iCBT with elimination of tumors. cDC1 vaccination coupled with iCBT identified specific CDR3 sequences in the tumor-infiltrating CD8+ T cells with potential therapeutic importance. This study …

Randomized Controlled Trial Of Enteral Vitamin D Supplementation (Vides) In Infants <28 Weeks Gestational Age Or <1000 G Birth Weight: Study Protocol, Mar Romero-Lopez, Jon E Tyson, Mamta Naik, Claudia Pedroza, Lindsay F Holzapfel, Elenir Avritscher, Ricardo Mosquera, Amir Khan, Matthew Rysavy

Faculty, Staff and Student Publications

BACKGROUND: Vitamin D is necessary to develop healthy lungs and other organs early in life. Most infants born before 28 weeks' gestation have low vitamin D levels at birth and a limited intake during the first month. Enteral vitamin D supplementation is inexpensive and widely used. The appropriate supplementation regimen for extremely preterm infants is controversial, and the effect of different regimens on their blood levels and outcomes is unclear.

METHODS: Randomized, blinded comparative effectiveness trial to compare two vitamin D supplementation regimens for inborn infants(400 IU/day with established feedings) or increased supplementation (800 IU/day with any feedings) during the …

Ten Myths About Artificial Intelligence In Education, Louie Giray

Higher Learning Research Communications

Objectives: I analyze, deconstruct, and debunk prevalent misconceptions about artificial intelligence (AI) in education.

Methods: This study identifies and presents ten common myths about AI in education, followed by concise explanations that counter each myth with the corresponding reality, relying on credible sources and evidence.

Results: AI does not replace educators; it lacks the vital human qualities crucial for effective learning experiences. Thus, it can complement rather than substitute for educators. Physical classrooms remain pivotal for fostering student engagement, an element AI cannot fully replicate, challenging the notion of AI replacing the need for traditional classrooms. Despite excelling in specific …

Lessening Nurses' Burden: Ai Enhanced Fall Risk Prediction, Joanne Aberilla, Andrea Tilleman

All Publications

No abstract provided.

Re: Approval Letter For The Butte Priority Soils Operable Unit (Bpsou) Draft Final Quarterly Operations And Maintenance Report Butte Treatment Lagoon System – Fourth Quarter 2023 (Dated March 28, 2024), Emma Rott

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Comprehensive Analysis Of The Different Fields Of Law, Kaitlyn Lee

Honors Theses

Since American law developed in the early 1700s, lawyers have played a crucial role in society, advocating for justice and upholding the rule of law. Over the years the legal profession has evolved immensely and now offers many different fields for individuals to practice. People are often curious about the work that lawyers do and the education that is required to become an attorney. This thesis aims to satisfy the curiosity of those individuals by exploring many facets of the different areas of law. It will examine the unique and shared aspects of the various legal fields, detailing the specific …

Blood Phenylalanine Levels In Patients With Phenylketonuria From Europe Between 2012 And 2018: Is It A Changing Landscape?, Alex Pinto, Kirsten Ahring, Manuela Ferreira Almeida, Catherine Ashmore, Amaya Bélanger-Quintana, Alberto Burlina, Turgay Coşkun, Anne Daly, Esther Van Dam, Ali Dursun, Sharon Evans, François Feillet, Maria Giżewska, Hulya Gökmen-Özel, Mary Hickson, Yteke Hoekstra, Fatma Ilgaz, Richard Jackson, Alicja Leśniak, Christian Loro, Katarzyna Malicka, Michał Patalan, Júlio César Rocha, Serap Sivri, Iris Rodenburg, Francjan Van Spronsen, Kamilla Strączek, Ayşegül Tokatli, Anita Macdonald

School of Health Professions

Background: In 2011, a European phenylketonuria (PKU) survey reported that the blood phenylalanine (Phe) levels were well controlled in early life but deteriorated with age. Other studies have shown similar results across the globe. Different target blood Phe levels have been used throughout the years, and, in 2017, the European PKU guidelines defined new targets for blood Phe levels. This study aimed to evaluate blood Phe control in patients with PKU across Europe. Methods: nine centres managing PKU in Europe and Turkey participated. Data were collected retrospectively from medical and dietetic records between 2012 and 2018 on blood Phe levels, …

Evaluating The Impact Of A 2-Week Peer Enhanced E-Placement (Peep) On Preregistration Physiotherapy Students’ Attitudes, Self-Perceived Confidence, And Competence In Delivering Telerehabilitation, Christie Robinson, Sarah Buckingham, Jennifer Freeman

School of Health Professions

The COVID-19 pandemic instigated rapid digital transformation of physiotherapy practice and education. We explored whether pre-registration specific teaching and practical experience of telerehabilitation may improve student confidence and competence in delivering telerehabilitation and support its clinical acceptance. A 2-week Peer Enhanced E-Placement (PEEP) was developed and evaluated to explore its impact on third year physiotherapy student attitudes, perceived competence, and confidence in delivering telerehabilitation. Students completed a questionnaire (pre and post PEEP), rating perceived confidence and competence in four areas of telerehabilitation: assessment, intervention, communication, and problem solving. Group discussions explored the impact of the PEEP on their attitudes towards …

Evaluating The Impact Of A 2-Week Peer Enhanced E-Placement (Peep) On Pre-Registration Physiotherapy Students’ Attitudes, Self-Perceived Confidence, And Competence In Delivering Telerehabilitation., Christie Robinson, Sarah Buckingham, Jennifer Freeman

School of Health Professions

The COVID-19 pandemic brought about rapid digital transformation of physiotherapy practice and education. Previous studies have suggested a lack of knowledge, beliefs, and clinical acceptance of telerehabilitation as barriers to implementation and sustainability of telerehabilitation. Providing pre-registration specific teaching and practical experience of telerehabilitation may improve physiotherapy student confidence and competence in delivering telerehabilitation and support clinical acceptance of telerehabilitation in practice. A 2-week Peer Enhanced E-Placement (PEEP) was developed and evaluated to explore its impact on third year physiotherapy student attitudes, and perceived competence and confidence in delivering telerehabilitation. Students completed a Likert scale questionnaire, immediately pre and post …

Aging- And Alcohol-Associated Spatial Transcriptomic Signature In Mouse Acute Pancreatitis Reveals Heterogeneity Of Inflammation And Potential Pathogenic Factors, Rachel R Tindall, Yuntao Yang, Isabella Hernandez, Amy Qin, Jiajing Li, Yinjie Zhang, Thomas H Gomez, Mamoun Younes, Qiang Shen, Jennifer M Bailey-Lundberg, Zhongming Zhao, Daniel Kraushaar, Patricia Castro, Yanna Cao, W Jim Zheng, Tien C Ko

Faculty, Staff and Student Publications

The rapidly aging population is consuming more alcohol, leading to increased alcohol-associated acute pancreatitis (AAP) with high mortality. However, the mechanisms remain undefined, and currently there are no effective therapies available. This study aims to elucidate aging- and alcohol-associated spatial transcriptomic signature by establishing an aging AAP mouse model and applying Visium spatial transcriptomics for understanding of the mechanisms in the context of the pancreatic tissue. Upon alcohol diet feeding and caerulein treatment, aging mice (18 months) developed significantly more severe AAP with 5.0-fold increase of injury score and 2.4-fold increase of amylase compared to young mice (3 months). Via …

Neural Circuits Expressing The Serotonin 2c Receptor Regulate Memory In Mice And Humans, Hesong Liu, Yang He, Hailan Liu, Bas Brouwers, Na Yin, Katherine Lawler, Julia M Keogh, Elana Henning, Dong-Kee Lee, Meng Yu, Longlong Tu, Nan Zhang, Kristine M Conde, Junying Han, Zili Yan, Nikolas A Scarcelli, Lan Liao, Jianming Xu, Qingchun Tong, Hui Zheng, Zheng Sun, Yongjie Yang, Chunmei Wang, Yanlin He, I Sadaf Farooqi, Yong Xu

Children’s Nutrition Research Center Staff Publications

Declined memory is a hallmark of Alzheimer’s disease (AD). Experiments in rodents and human postmortem studies suggest that serotonin (5-hydroxytryptamine, 5-HT) plays a role in memory, but the underlying mechanisms are unknown. Here, we investigate the role of 5-HT 2C receptor (5-HT2CR) in regulating memory. Transgenic mice expressing a humanized HTR2C mutation exhibit impaired plasticity of hippocampal ventral CA1 (vCA1) neurons and reduced memory. Further, 5-HT neurons project to and synapse onto vCA1 neurons. Disruption of 5-HT synthesis in vCA1-projecting neurons or deletion of 5-HT2CRs in the vCA1 impairs neural plasticity and memory. We show that a selective 5-HT2CR agonist, …

Berkeley Pit And Discharge Pilot Project Temperature Evaluation, 2023 Update, Mark Thompson, Dave Griffis

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Comments For The 2024 Butte Priority Soils (Bpsou) Residential Metals Abatement Program (Rmap) – Quality Assurance Project Plan (Qapp) For Non-Residential Parcels And Residential Daycares (Dated May 23, 2024), Molly Roby

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Comment/Approval Letter For The Butte Priority Soils Operable Unit (Bpsou) Draft Final Insufficiently Reclaimed Sites Field Sampling Plans (Fsps) For Bres No. 121 – Travona Dump, Bres No. 125 – Child Harold-2 Dump, Bres No. 34e – Eveline Dump East, Bres No. 45 – Garfield, Bres No. 49 – Old Glory, Bres No. 52 – Moscow, Bres No. 74 – West Gagnon Dump, And Bres No. 78 Original Mine (Dated May 7, 2024), Molly Roby

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Re: Approval Letter For The Butte Priority Soils Operable Unit (Bpsou) Draft Final 2024 Residential Metals Abatement Program Park Soil Sampling Field Sampling Plan Submittal #14 [Silver Bow Homes Park, Butte Koa, Fairmont Rv Park, And Cccs Gymnasium] (Dated May 10, 2024), Molly Roby

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.