Digital Commons Network^™

Regulatory Complexity Of Cellular Differentiation In Candida Albicans Revealed Through Systematic Screening Of Protein Kinase Mutants, Michael C Lorenz

Faculty, Staff and Student Publications

A recent study in mBio reports the construction and preliminary screening of a library containing mutants of 99 of the 119 predicted protein kinases in Candida albicans (the majority of the remaining 20 are probably essential) (J. Kramara, M.-J. Kim, T. L. Ollinger, L. C. Ristow, et al., mBio e01249-24, 2024, https://doi.org/10.1128/mbio.01249-24). Using a quantitative competition assay in 10 conditions that represent nutritional, osmotic, cell wall, and pH stresses that are considered to model various aspects of the host environment allowed them to phenotypically cluster kinases, which highlight both the integration and specialization of signaling pathways, suggesting novel functions …

Role Of The Antiparallel Double-Stranded Filament Form Of Ftsa In Activating The Escherichia Coli Divisome, Abbigale Perkins, Mwidy Sava Mounange-Badimi, William Margolin

Faculty, Staff and Student Publications

The actin-like FtsA protein is essential for function of the cell division machinery, or divisome, in many bacteria including Escherichia coli. Previous in vitro studies demonstrated that purified wild-type FtsA assembles into closed mini-rings on lipid membranes, but oligomeric variants of FtsA such as FtsAR286W and FtsAG50E can bypass certain divisome defects and form arc and double-stranded (DS) oligomeric states, respectively, which may reflect conversion of an inactive to an active form of FtsA. However, it remains unproven which oligomeric forms of FtsA are responsible for assembling and activating the divisome. Here, we used an in vivo crosslinking assay …

Production, Purification, And Specificity Serologically Determination Of Immunoglobulin-Y (Igy) From Chicken Eggs Against Clostridium Tetani Toxoid, Kürşat Teti̇k, Bariş Sareyyüpoğlu

Turkish Journal of Veterinary & Animal Sciences

Tetanus is a life-threatening bacterial disease of humans and many animal species caused by the neurotoxin tetanospasmin (TeNT) produced by Clostridium tetani. Antitoxins obtained from horses and humans are primarily used to treat this disease. However, there are several clinical side effects and disadvantages associated with the use of these antitoxins. Current techniques for diagnosing tetanus use monoclonal antibodies produced in mice. These antibodies have several advantages, such as homogeneity and specificity. In contrast, a notable feature of polyclonal antibodies, especially egg yolk antibodies (IgYs) extracted from poultry, is that they can be generated in greater quantities than mammalian antibodies …

Cover And Contents

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Adaptation And Evaluation Of Guideline-Based Family-Based Behavioral Treatment For Overweight And Obesity In Childhood Survivors Of Acute Lymphoblastic Leukemia And Lymphoma, Jessica Jakubiak

Arts & Sciences Graduate Student Theses and Dissertations

Children who survive acute lymphoblastic leukemia (ALL) and lymphoma are at considerable risk for chronic, treatment-related medical complications known as “late effects.” Not only are these late effects significant in and of themselves, but many are exacerbated by excess weight, a particularly concerning combination given the elevated rates of overweight and obesity within this population. Although the therapeutic regimens (e.g., steroid “pulses”) endured by children who survive ALL and lymphoma may contribute to excess weight, accumulating evidence also demonstrates the development of poor dietary habits and inadequate levels of physical activity during cancer treatment. Such weight-related health behaviors appear to …

Parental Experiences And Health Education Perceptions Raising Children With Orofacial Clefts, Ama Agyeibea Amuasi

Walden Dissertations and Doctoral Studies

Congenital abnormalities, such as cleft lip and/or palate (CL/P), are a significant global public health issue that contribute to newborn morbidity and mortality, difficulties in social integration, psychological problems for affected persons, parents, and families. Available health education information and resources on CL/P care for parents and families in Ghana are however minimal. The purpose of this qualitative study was to explore the health education perceptions of parents of children born with CL/P to identify and understand experiences of common psychosocial challenges, potential risk, and protective factors used by parents. Five research questions were designed to examine the health education …

A Comparative Analysis Of Human And Machine Translation Quality, Catherine Marshall

Theses and Dissertations

A common question raised by both translators and Machine Translation developers is Will Machine Translation (MT) ever attain the level of Human Translation (HT) quality? Researchers have argued that MT will likely never replace HT completely because MT systems cannot anticipate all the specifications for possible translation projects (Melby & Hague, 2019) and because MT systems lack cultural understanding (Latief et al., 2020). Researchers have also argued that the use of machines should be focused on augmenting humans, rather than trying to imitate them (Brynjolfsson, 2022). The purpose of this study is to determine how the quality of MT differs …

Barriers And Facilitators When Implementing Midwifery Continuity Of Carer: A Narrative Analysis Of The International Literature, Aimee Louise Middlemiss, Susan Channon, Julia Sanders, Sara Kenyon, Rebecca Milton, Tina Prendeville, Susan Barry, Heather Strange, Aled Jones

School of Nursing and Midwifery

BackgroundMidwifery continuity of carer (MCoC) is a model of care in which the same midwife or small team of midwives supports women throughout pregnancy, birth and the postnatal period. The model has been prioritised by policy makers in a number of high-income countries, but widespread implementation and sustainability has proved challenging.MethodsIn this narrative review and synthesis of the global literature on the implementation and sustainability of midwifery continuity of carer, we identify barriers to, and facilitators of, this model of delivering maternity care. By mapping existing research evidence onto the Consolidated Framework for Implementation Research (CFIR), we identify factors for …

The George-Anne Inkwell Edition, Georgia Southern University

The Inkwell

No abstract provided.

The George-Anne, Georgia Southern University

The George-Anne

• The Eagle Experience
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• University System of Georgia Tuition Increase and Online Learning Fee take Effect this Fall
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• Dear Freshman Fayth
• Savannah's must-try Coffee and Dining
• Upcoming events at Armstrong
• How to have fun in Savannah
• Meet the 2024 Inkwell Staff
• Be on the Look-out!

Assessing Healthcare Interventions: Impact Of Covid-19 Precautions On Hospital Acquired Infections, Judeil Krlan Teus, Lucinda Mithen, Heidi Green, Alison Hutton, Ritin Fernandez

International Nursing Research Congress (INRC)

Personal protective equipment (PPE) has played a fundamental role in protecting healthcare workers and patients, but its effectiveness in reducing hospital-acquired infections (HAIs) during the pandemic remains a subject of debate. This systematic review and meta-analysis explores the impact of COVID-19 precautions on HAIs and its implications for future practice.

Persistence Of Daptomycin-Resistant And Vancomycin-Resistant Enterococci In Hospitalized Patients With Underlying Malignancies: A 7-Year Follow-Up Study, Lynn El Haddad, Georgios Angelidakis, Yuting Zhai, Layale Yaghi, Cesar A Arias, Samuel A Shelburne, Kwangcheol Casey Jeong, Roy F Chemaly

Faculty, Staff and Student Publications

Vancomycin-resistant enterococci (VRE) commonly colonize the gut of individuals with hematologic malignancies or undergoing hematopoietic cell transplant (HCT) and may cause bacteremia. In 2012, we identified VRE isolates from patients and patients' rooms and showed transmission networks of highly genetically related daptomycin-resistant (DR)-VRE strains. This is a follow-up study performing whole-genome sequencing (WGS) and phylogenetic analyses on 82 clinical VRE strains isolated from stools and blood cultures of patients with leukemia and HCT between 2015 and 2019. Here, we observed transmission of highly genetically related strains between rooms on the same or on different floors, including a DR-VRE strain identified …

Functional Genomics Approaches To Understanding The Impact Of Common And Rare Risk Variants On Microglia Function In Neurodegenerative Diseases, Shih-Feng You

Arts & Sciences Graduate Student Theses and Dissertations

Genome-wide association studies (GWAS) have identified more than 70 genetic loci that are linked to Alzheimer disease (AD) risk. Many of these risk genes are involved in microglial function, including MS4A4A, TREM2, and PLD3, suggesting a relationship between microglial phagocytosis, metabolism, inflammation, and AD pathogenesis. However, the mechanisms by which AD risk genes alter microglia function and contribute to disease remains inadequately explored. In this dissertation, I leveraged functional genomics methods to understand the impact of MS4A4A, TREM2, and PLD3 on microglia function in stem cell and mouse models as well as human brain tissue. MS4A4A is a microglial gene …

Insights From Open Conformations Of Ecmsck, Htrpv4, And Hhcn1 Into The Structural Basis Of Ligand-Regulated Ion Channel Gating, Jonathan Mount

Arts & Sciences Graduate Student Theses and Dissertations

Ion channels are found in all domains of life and play fundamental roles in cellular signaling and response to environmental cues. The ability to accommodate the selective and rapid passage of ions across a cell membrane is conferrable by a myriad of protein folds with no singular one-size-fits-all approach. With the advent of the cryo-EM resolution revolution, substantial interest has developed for determining the molecular structures of conformational states that correspond to conductive and nonconductive forms of ion channels. By gaining insight into the structural mechanisms for ion channel gating, we can potentially reveal binding pockets for targeted small molecule …

Tfeb Activation Hallmarks Antigenic Experience Of B Lymphocytes And Directs Germinal Center Fate Decisions, Matthias Münchhalfen, Richard Görg, Michael Haberl, Jens Löber, Jakob Willenbrink, Laura Schwarzt, Charlotte Höltermann, Christian Ickes, Leonard Hammermann, Jan Kus, Björn Chapuy, Andrea Ballabio, Sybille D Reichardt, Alexander Flügel, Niklas Engels, Jürgen Wienands

Duncan NRI Faculty and Staff Publications

Ligation of the B cell antigen receptor (BCR) initiates humoral immunity. However, BCR signaling without appropriate co-stimulation commits B cells to death rather than to differentiation into immune effector cells. How BCR activation depletes potentially autoreactive B cells while simultaneously primes for receiving rescue and differentiation signals from cognate T lymphocytes remains unknown. Here, we use a mass spectrometry-based proteomic approach to identify cytosolic/nuclear shuttling elements and uncover transcription factor EB (TFEB) as a central BCR-controlled rheostat that drives activation-induced apoptosis, and concurrently promotes the reception of co-stimulatory rescue signals by supporting B cell migration and antigen presentation. CD40 co-stimulation …

Infant And Adult Human Intestinal Enteroids Are Morphologically And Functionally Distinct, Grace O Adeniyi-Ipadeola, Julia D Hankins, Amal Kambal, Xi-Lei Zeng, Ketki Patil, Victoria Poplaski, Carolyn Bomidi, Hoa Nguyen-Phuc, Sandra L Grimm, Cristian Coarfa, Fabio Stossi, Sue E Crawford, Sarah E Blutt, Allison L Speer, Mary K Estes, Sasirekha Ramani

Faculty, Staff and Students Publications

UNLABELLED: Human intestinal enteroids (HIEs) are gaining recognition as physiologically relevant models of the intestinal epithelium. While HIEs from adults are used extensively in biomedical research, few studies have used HIEs from infants. Considering the dramatic developmental changes that occur during infancy, it is important to establish models that represent infant intestinal characteristics and physiological responses. We established jejunal HIEs from infant surgical samples and performed comparisons to jejunal HIEs from adults using RNA sequencing (RNA-Seq) and morphologic analyses. We then validated differences in key pathways through functional studies and determined whether these cultures recapitulate known features of the infant …

Development Of A Novel Amplifiable System To Quantify Hydrogen Peroxide In Living Cells, Lingfei Wang, Hanfeng Lin, Bin Yang, Xiqian Jiang, Jianwei Chen, Sandipan Roy Chowdhury, Ninghui Cheng, Paul A Nakata, David M Lonard, Meng C Wang, Jin Wang

Faculty, Staff and Students Publications

Although many redox signaling molecules are present at low concentrations, typically ranging from micromolar to sub-micromolar levels, they often play essential roles in a wide range of biological pathways and disease mechanisms. However, accurately measuring low abundant analytes has been a significant challenge due to the lack of sensitivity and quantitative capability of existing measurement methods. In this study, we introduced a novel chemically induced amplifiable system for quantifying low-abundance redox signaling molecules in living cells. We utilized H2O2 as a proof-of-concept analyte and developed a probe that quantifies cellular peroxide levels by combining the NanoBiT system with androgen receptor …

Clinical And Radiographic Outcomes After Mechanical Thrombectomy In Medium-Vessel Posterior Cerebral Artery Occlusions: Subgroup Analysis From Star, Eyad Almallouhi, Matthew C Findlay, Ilko Maier, Pascal Jabbour, Joon-Tae Kim, Stacey Quintero Wolfe, Ansaar Rai, Robert M Starke, Marios-Nikos Psychogios, Amir Shaban, Nitin Goyal, Shinichi Yoshimura, Hugo Cuellar, Brian Howard, Ali Alawieh, Ali Alaraj, Mohamad Ezzeldin, Daniele G Romano, Omar Tanweer, Justin Mascitelli, Isabel Fragata, Adam Polifka, Fazeel Siddiqui, Joshua Osbun, Roberto Crosa, Charles Matouk, Min S Park, Michael R Levitt, Waleed Brinjikji, Mark Moss, Ergun Daglioglu, Richard Williamson, Pedro Navia, Peter Kan, Reade De Leacy, Shakeel Chowdhry, David J Altschul, Alejandro Spiotta, Ramesh Grandhi

Faculty, Staff and Students Publications

BACKGROUND: Whereas mechanical thrombectomy (MT) has become standard-of-care treatment for patients with salvageable brain tissue after acute stroke caused by large-vessel occlusions, the results of MT in patients with medium-vessel occlusions (MEVOs), particularly in the posterior cerebral artery (PCA), are not well known.

METHODS: Using data from the international Stroke Thrombectomy and Aneurysm Registry (STAR), we assessed presenting characteristics and clinical outcomes for patients who underwent MT for primary occlusions in the P2 PCA segment. As a subanalysis, we compared the PCA MeVO outcomes with STAR's anterior circulation MeVO outcomes, namely middle cerebral artery (MCA) M2 and M3 segments.

RESULTS: …

Assessing Patterns Of Metazoans In The Global Ocean Using Environmental Dna, Nathan R. Geraldi, Silvia G. Acinas, Intikhab Alam, Josep M. Gasol, María Luz Fernández-De-Puelles, Caterina R. Giner, Santiago Hernández León, Ramiro Logares, Ramon Massana, Pablo Sánchez, Vladimir Bajic, Takashi Gojobori, Carlos M. Duarte

Research outputs 2022 to 2026

Documenting large-scale patterns of animals in the ocean and determining the drivers of these patterns is needed for conservation efforts given the unprecedented rates of change occurring within marine ecosystems. We used existing datasets from two global expeditions, Tara Oceans and Malaspina, that circumnavigated the oceans and sampled down to 4000 m to assess metazoans from environmental DNA (eDNA) extracted from seawater. We describe patterns of taxonomic richness within metazoan phyla and orders based on metabarcoding and infer the relative abundance of phyla using metagenome datasets, and relate these data to environmental variables. Arthropods had the greatest taxonomic richness of …

Immunological Correlates Of Protection Mediated By A Whole Organism,Cryptococcus Neoformans, Vaccine Deficient In Chitosan, Charles A Specht, Ruiying Wang, Lorena V N Oliveira, Maureen M Hester, Christina Gomez, Zhongming Mou, Diana Carlson, Chrono K Lee, Camaron R Hole, Woei C Lam, Rajendra Upadhya, Jennifer K Lodge, Stuart M Levitz

2020-Current year OA Pubs

UNLABELLED: The global burden of infections due to the pathogenic fungus

IMPORTANCE: The fungus

Development Of Vla4 And Cxcr4 Antagonists For The Mobilization Of Hematopoietic Stem And Progenitor Cells, Peter G Ruminski, Michael P Rettig, John F Dipersio

2020-Current year OA Pubs

The treatment of patients diagnosed with hematologic malignancies typically includes hematopoietic stem cell transplantation (HSCT) as part of a therapeutic standard of care. The primary graft source of hematopoietic stem and progenitor cells (HSPCs) for HSCT is mobilized from the bone marrow into the peripheral blood of allogeneic donors or patients. More recently, these mobilized HSPCs have also been the source for gene editing strategies to treat diseases such as sickle-cell anemia. For a HSCT to be successful, it requires the infusion of a sufficient number of HSPCs that are capable of adequate homing to the bone marrow niche and …

Sac1 Links Phosphoinositide Turnover To Cryptococcal Virulence, Elizabeth A Gaylord, Hau Lam Choy, Guohua Chen, Sydney L Briner, Tamara L Doering

2020-Current year OA Pubs

No abstract provided.

Neuronal Activity In The Gustatory Cortex During Economic Choice, Ahmad Jezzini, Camillo Padoa-Schioppa

2020-Current year OA Pubs

An economic choice entails computing and comparing the values of individual offers. Offer values are represented in the orbitofrontal cortex (OFC)-an area that participates in value comparison-but it is unknown where offer values are computed in the first place. One possibility is that this computation takes place in OFC. Alternatively, offer values might be computed upstream of OFC. For choices between edible goods, a primary candidate is the gustatory region of the anterior insula (gustatory cortex, GC). Here we recorded from the GC of male rhesus monkeys choosing between different juice types. As a population, neurons in GC represented the …

5Β-Dihydrosteroids: Formation And Properties, Trevor M Penning, Douglas F Covey

2020-Current year OA Pubs

5β-Dihydrosteroids are produced by the reduction of Δ

Beyond Mindset : Investigating Neural And Psychological Mechanisms Of Strategic Mindset, Growth Mindset And Feedback Learning, Yaqi Yang

Lingnan Theses (MPhil & PhD)

Extensive research has explored the benefits of the growth mindset over the past decades. Recently, researchers have turned their attention to the strategic mindset, a new concept with the potential to positively impact cognitive and behavioural change. While some literature suggests that both mindsets are associated with feedback learning, robust evidence is lacking, and the relationship between the three factors remains unknown. This thesis aims to investigate the neural and psychological mechanisms underlying the strategic mindset, growth mindset, and feedback learning through three distinct studies.

In Study 1, utilising a self- and other-rating paradigm, we examined neural and behavioural evidence …

The Colorado Center For Rural Education: Mitigating The Teacher Shortage In Colorado, Joey Laurx, Robert Fulton, Rosanne Fulton

Journal of Educational Research and Innovation

Colorado’s rural communities face challenges recruiting and retaining qualified teachers, leaders, and special service providers. The Colorado Center for Rural Education (CCRE) has created and implemented a comprehensive system for professional development of effective educators for rural school districts. The CCRE has expanded educator pathway programs, supported teacher preparation students who commit to student teaching in rural Colorado, facilitated a rural teacher leaders program that prepares teachers for expanded professional roles, developed a coordinated professional development network for rural educators, and cultivated rural education advocacy, scholarship, and grant writing. The CCRE’s outcomes include more student teachers, alternative teacher licensure candidates, …

Journeying In The Confessions And The Aeneid, Griffin Blood

Parnassus: Classical Journal

No abstract provided.

Augmenting Images With Contextual Texture Cues To Assist Viewers With Color Vision Deficiency, Shumeet Baluja, David Marwood

Defensive Publications Series

This disclosure describes techniques that enable a viewer with color vision deficiency to better understand color images by automatically mapping problematic colors to distinguishable textures - distinctive black-and-white patterns corresponding to particular colors. A deep learning model selects appropriate textures for each color such that textures are consistent across images. Textures inform the viewer of similar and dissimilar colors and are only applied where needed. The original color image is augmented with textures such that both color-deficient users and users with regular vision can understand the image. The techniques can be used to display images on any device and can …

Educational Chatbot Development Informed By Clinical Simulations, Alicia Drelick, Casey L. Woodfield, Justin E. Freedman

College of Education Departmental Research

This manuscript introduces an innovative approach to developing educational chatbots informed by clinical simulations to enhance learning experiences. With the rapid interaction of generative AI in education, this work provides a structured process to creating chatbots that simulate interactions for educational purposes. The four development phases, Conceptualization, Protocol Design, Technical Design, and Trials and Revisions are grounded in the foundational practices of clinical simulations. This approach bridges gaps between theory and practical application of emerging technologies while emphasizing the role of humans at the center of AI-enhanced learning. In addition to technical guidance, pedagogical strategies for introducing chatbots to students …

Thinking Long-Term: Nursing Education On Long-Acting Injectable Antipsychotics, Arslan Rashid

DNP Qualifying Manuscripts

Schizophrenia cases and their associated healthcare costs have drastically increased over time in the U.S. Antipsychotic non-adherence is a primary contributor to the high rates of relapse and rehospitalization among the schizophrenic adult population. The development of long-acting injectable antipsychotics (LAIs) has positively impacted medication adherence, however, this treatment modality is still underemployed in practice due to barriers, such as physician and patient negative attitudes toward LAIs and lack of education. Nurses are in a great position to address these barriers as they serve as mediators between providers and patients. This literature review aims to discover evidence that LAI education …