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Treatment Sequence With Tebentafusp And Immune Checkpoint Inhibitors In Patients With Metastatic Uveal Melanoma And Metastatic Gna11/Gnaq Mutant Melanocytic Tumors, Florentia Dimitriou, Marlana Orloff, Erica Koch Hein, Phil Cheng, Isaac Hughes, Ester Simeone, Kamaneh Montazeri, Piyush Grover, Inderjit Mehmi, Camille Gerard, Caroline Gaudy-Marqueste, Jean-Jacques Grob, Olivier Michielin, Omid Hamid, Georgina Long, Ryan Sullivan, Ellen Kapiteijn, Douglas Johnson, Paolo Ascierto, Anthony Joshua, Richard Carvajal, Marcus Butler, Jessica Hassel, Reinhard Dummer

Kimmel Cancer Center Faculty Papers

BACKGROUND: Metastatic uveal melanoma (mUM) is rare. Immune checkpoint inhibitors (ICIs) have shown modest efficacy in mUM. Tebentafusp prolonged overall survival (OS) in a phase 3 study. We aimed to investigate the efficacy and safety of the sequence of tebentafusp and ICIs.

METHODS: Patients with HLA-A * 02:01 positive mUM, or metastatic GNA11/GNAQ mutant melanocytic tumors treated with tebentafusp followed by ICIs (group 1) or the inverse sequence (group 2) at any treatment line were retrospectively identified. The primary objective was OS rate at 2 years.

RESULTS: 131 patients were included; 51 in group 1 and 80 in group 2. …

Getting It “Right”: Educators’ Experiences With School Diversity In A Gentrifying Neighborhood, Jennifer B. Ayscue, Kfir Mordechay, Gage F. Matthews, Julie Whetzel

All Faculty Open Access Publications

Schools in gentrifying neighborhoods often experience demographic changes in enrollment. The purpose of this qualitative holistic case study is to describe how leaders and teachers in a diversifying elementary school in a gentrifying neighborhood perceive and experience diversity. Drawing on Turner’s value of diversity framework, we use inductive coding to analyze interviews and also use documents to inform our findings. Although Greenleaf was striving to be intentionally diverse, consensus did not exist about the meaning of “diversity” or the desired form of diversity. Challenges associated with decentering Whiteness and resisting upholding the racial contract existed as educators worked to establish …

Avoiding Four Fallacies In The Fair Allocation Of Influenza Countermeasures, Govind Persad, Johan L. Dellgren, Ezekiel J. Emanuel

Publications

No abstract provided.

Effects Of Liraglutide On The Gut Bacterial Community, Jami Anne Bull

Graduate Theses/Dissertations

Overweight and obesity are major health concerns associated with metabolic disorders and gut dysbiosis. Treatment options such as GLP-1 receptor agonists, like liraglutide, promote weight loss and improve insulin sensitivity; however, their impact on the gut bacterial community remains unclear. This study investigated the effects of liraglutide on the gut bacterial community of diet-induced obese (DIO) mice using Illumina DNA sequencing of the bacterial 16S rRNA gene. My study used twelve-week-old male C57BL/6J DIO mice. They were placed on a high-fat diet and treated with liraglutide (0.2 mg/kg) via daily subcutaneous injection for 14 Days. High-fat and low-fat diets control …

The Influence Of Flower-Microbe Interactions On Bumble Bee Behavior And Gut Microbiome Composition, Success Chiamaka Ekemezie

Graduate Theses/Dissertations

This thesis examines how microbial interactions in floral nectar and environmental variation influence bumble bee (Bombus spp.) foraging behavior and gut microbiome composition. Pollen germination experiments and behavioral assays in the lab tested whether Metschnikowia reukaufii (yeast) and Acinetobacter nectaris (bacteria) induce pollen germination and bursting in nectar and affect bee behavior subsequently. Both microbes reduced intact pollen, but bees did not alter flower preference. Furthermore, the study also assessed how floral and bee community composition shaped gut microbiome composition across six Missouri prairies. Using amplicon-sequenced fungal ITS and bacterial 16S rRNA genes, I found that fungal microbiome composition varied …

Cgrp Modulation Of Intracellular Calcium Levels In Trigeminal Ganglion Neurons And Glia, Nicole M. Nalley

Graduate Theses/Dissertations

Elevated levels of calcitonin gene-related peptide (CGRP) are implicated in migraine and TMD pathology, but its effects on the excitability state of Aδ and C-fiber neurons and glia have not been investigated. The goal of this study was to determine changes mediated by CGRP on intracellular calcium levels in trigeminal ganglion neurons and glia in response to depolarizing stimulation. Intracellular calcium levels were determined in Aδ and C-fiber neurons of primary trigeminal ganglion cultures obtained from neonatal Sprague Dawley rats using Fura-2 and fluorescent microscopy. Cells were left untreated or preincubated for 2 hours with CGRP, then incubated with the …

Spatial And Temporal Shifts In Bacterial Communities And Nutrient Removal In Two Springfield Wastewater Facilities, Rubina Sherchan

Graduate Theses/Dissertations

Bacterial communities play a central role in the biological nutrient removal and overall performance of wastewater treatment plants (WWTPs). However, very few is known about the spatial and seasonal dynamics of these communities. This study explores the spatial and seasonal variations in bacterial community composition across multiple treatment stages in two municipal WWTPs—Southwest (SWTP) and Northwest (NWTP)—in Springfield, Missouri. Using 16S rRNA gene amplicon sequencing, I analyzed 87 samples collected during summer and winter of 2024 from influent, sludge, aeration basins, clarifiers, and final effluent stages. I retrieved 16.4 million (summer) and 3.2 million (winter) sequences. Proteobacteria dominated both seasons …

Preventing Chemical And Biological Attacks: Enhanced Threats Requiring Enhanced Solutions, Alex Elliot Ottoni-Belval

Graduate Theses/Dissertations

Chemical weapons (CW) and biological weapons (BW) are becoming more dangerous for experts to employ and more accessible to amateurs. Despite CW and BW attacks throughout history that killed and maimed millions of people, the international community has failed to establish meaningful consequences to prevent them. Agreements such as the Chemical Weapons Convention and the Biological Weapons Convention vow to eliminate and reduce the effects of CWs and BWs but lack respected response precedents and capabilities. The limited and inconsistent enforcement has inadvertently allowed CWs and BWs to become plausible weapons, especially to gain asymmetric advantages, while modern developments exasperate …

A Novel Deterrence Strategy: Denial Through Space And Cyberspace, Nathan A. Heard

Graduate Theses/Dissertations

The United States faces numerous security threats now and in the coming decades. Authoritarian and revisionist powers such as China and Russia, along with regional proxies, are destabilizing the international order. The ability of the United States to adequately address these present and future threats is constrained by conventional and nuclear forces in need of recapitalization, a rising national deficit, disagreements over the direction of U.S. foreign policy, and a defense industrial enterprise that has its roots in the Cold War. In a multi-peer threat environment, this situation erodes U.S. credibility and is an invitation for aggression. Coercion, coordinated multi-theater …

Investigating Social Attenuation Of The Stress Response In Juvenile Northern Cottonmouths (Agkistrodon Piscivorus), Alyssa Owens

Graduate Theses/Dissertations

When social organisms encounter a stress-inducing event, the presence of a conspecific can dampen the physiological and/or behavioral response elicited by the stressor. This concept, termed ‘social buffering’, has been well documented in mammals, but there are few studies investigating this phenomenon in organisms traditionally perceived as asocial, including reptiles. However, the paradigm is shifting, and social behavior is now more widely recognized in reptiles, including snakes. In this study I tested whether juvenile Northern Cottonmouths (Agkistrodon piscivorous) experience social buffering by measuring changes in heart rate in snakes following an acute stressor with and without the presence …

Providing Preschool Classrooms With Sensory Kits To Measure The Effectiveness Of Sensory Input On Motor Functioning And Sensory Integration, Elena Tedeschi

Graduate Theses/Dissertations

This study measured the effectiveness of sensory kits in preschool classrooms. Preschool children ages 2 years 9 months to 4 years old were divided into a control group classroom that did not receive sensory kits and an experimental group classroom that did receive sensory kits. Both groups were assessed in a pre-test and a post-test using 10 subsets of the Evaluation of Ayres Sensory Integration assessment to measure sensory integration and using the Peabody Developmental Motor Scale to evaluate motor progression. The control group post-test scores moved farther from norm and average scores, while the experimental group post-test scores showed …

Alpha-Synuclein Interaction With Gedunin, Tony Matundura Nyabayo

Graduate Theses/Dissertations

Parkinson’s disease (PD) and other Proteinopathies develop when α-synuclein misfolds and aggregates into toxic amyloids. While existing treatments for PD are primarily focused on managing its symptoms, a viable solution for slowing down the progress of Parkinson’s disease involves targeting the toxic α-synuclein amyloids. Gedunin, a natural inhibitor of heat shock protein 90, has been extensively used to treat malaria. Also, recent research investigations have shed light on its potential beyond malaria therapy, indicating that Gedunin may offer a possible solution for treating a variety of neurodegenerative diseases. Using plate-based assays, we examined how Gedunin influences α-synuclein fibrillation and its …

Lc-Ms Discriminates Between Effects Of Sulfur Nanoparticles Vs Molybdenum Disulfide Nanoparticles On Lettuce (Lactuca Sativa), Mary Fakunle

Graduate Theses/Dissertations

Nanotechnology is gaining attention in agriculture, majorly due to their potential benefits in farming practices. Sulfur and molybdenum disulfide nanoparticles are reported to enhance plant growth, protection, and nutrient delivery. However, there is a need to understand how nano-fertilizers affect the metabolites and nutritional benefits of plants. Here, lettuce was cultivated under greenhouse in one of three treatments: control, 50 ppm S NPs, and 20 ppm MoS2 NPs. A method was developed on Liquid Chromatography – Mass Spectrometry (LC-MS) instrument to quantify the concentration of amino acids and antioxidants in the harvested lettuce plants. Inductively Coupled Plasma – Mass Spectrometry …

Investigating The Presence Of Individuality And Behavioral Syndromes In Northern Cottonmouths (Agkistrodon Piscivorus), Joshua Lowy

Graduate Theses/Dissertations

Within the last 15 ̶20 years, research on behavioral syndromes has seen a surge in prominence and popularity in behavioral ecology, revealing that many animals exhibit individually consistent behavioral tendencies. Behavioral syndromes occur when behavioral traits are correlated with one another within the same context or across different contexts. Despite new appreciation for the ecological, evolutionary, and conservation implications of behavioral syndrome data, the taxonomic breadth of studies is uneven. Snakes are underrepresented within squamate reptiles and, among venomous species, only two rattlesnakes (Crotalus) have been investigated for evidence of behavioral syndromes. My study investigates individuality and the presence of …

A Mystery Of Microecology: Revealing The Ecological Relationship Between Allorhizobium Vitis And Missouri Native Grape Species, Hunter White

Graduate Theses/Dissertations

Crown gall disease can be very detrimental to grape and wine producers around the world by reducing vine vigor and yield. It is caused by the bacteria Allorhizobium vitis (A. Vitis) carrying a tumor inducing (Ti) plasmid. The A. vitis bacteria have been found in soil and cane tissues in vineyards around the world, and infect all cultivated grapes. However, there have been no reports of crown gall on native wild grapevines, and very little is known about the ecology of A. vitis in native ecosystems. I have sampled cambium tissues from three native wild grape species of …

Sensory Processing And Anxiety In Bipolar Disorder, Lyric Arvizu

Graduate Theses/Dissertations

Past research suggests that individuals with bipolar disorder often experience differences in sensory gating and sensory processing, and similar patterns have been observed in anxiety disorders. Given that anxiety is highly comorbid with bipolar disorder, the present study examined how these conditions relate to self-reported sensory processing differences. A total of 132 participants (88 female), including 43 with bipolar disorder, were included in the final sample. Anxiety symptomatology was measured via the State-Trait Anxiety Inventory (STAI) Form Y. Sensory processing questionnaires included the Sensory Gating Inventory (SGI) and the Adult Sensory Processing Scale (ASPS). Correlations, t-tests, a multivariate analysis of …

Fostering Effective Practice: Using Metacognition Logs In The Private Voice Studio, Colleen C. Pingel

Graduate Theses/Dissertations

ABSTRACT

The purpose of this case study was to examine the short-term impact of metacognition instruction on the practice of a single voice student in a private studio. Across four lessons, the student received explicit instruction in metacognitive strategies and applied them through guided practice logs supporting planning, monitoring, adjusting, and reflecting. Each lesson began with a review and discussion of the practice logs. The teacher-researcher recorded these discussions to capture the student’s thinking and maintained a journal to document observations, instructional decisions, and reflections. Data analysis involved primarily qualitative methods focused on reflections, discussions, and practice logs. Quantitative measures, …

Constitutional Crossroads: The Urgency For First Amendment Protections Over Parental Rights In Florida School Libraries, Rindaanne H. Riccio

Western New England Law Review

The First Amendment rights of children and students are seen as coextensive with those of adults. . .unless their parents say otherwise. Florida’s recent passing of HB 1069 is considered to be the latest champion in parental autonomy rights in education. Famously associated with Governor DeSantis’ promise to protect Florida’s children through book removals, the law allows parents to review, object to, and limit students’ access to books in school libraries. This is an expansion of parental rights beyond what America has traditionally recognized. These book limitations are now being called into question in a Florida federal district court. The …

Medication-Assisted Treatment Expansion For Opioid-Use Disorder In The Oregon Department Of Corrections And Post-Release From Prison, Justin Haddad

Western New England Law Review

In 2021, nearly 80,000 people died from an opioid-related overdose. This startling statistic was the impetus that led many states to try to combat opioid use disorder (OUD) over recent years. Many of these states have realized that OUD bleeds into their correctional facilities and have decided to offer Medication-Assisted Treatment (MAT) to incarcerated individuals. Oregon was once considered the vanguard of progressive policymaking, most recently when it enacted Measure 110 (M110), which decriminalized certain drug possession and provided treatment to people suffering from substance abuse. Oregon has recently initiated a pilot program to integrate MAT into its correctional facilities. …

From The Battlefield To The Asylum: The Social Construction Of Disability In Italy, 1900 - 1950, Brandie Crisp Robb

Graduate Theses/Dissertations

The Italian social construction of disability reflects a complex interplay between social, religious, political, and religious influences present during the first half of the twentieth century. While Western European and American disability discourse remained similar, those influences unique to Italy resulted in the formation of two distinct disability discourses during the early twentieth century: that of the heroic physically disabled veteran who sacrificed everything for the good of Italy, and the eugenically unfit Italian associated with dependency and uselessness. By combining a theoretical framework that includes critical disability theory with historical methodology, the following thesis demonstrates how the emerging disability …

An Investigation Of The Physiological And Behavioral Correlates Of Mental Imagery, Matthew Daniel Carroll

Graduate Theses/Dissertations

Many conventional theories hold mental imagery to be fundamental to self-regulation. Recently, a current of research into mental imagery has developed, with the terms anaurelia and aphantasia being coined for the inability to experience, respectively, auditory and visual thought content, yet little is known about the impact that such individual differences have on persons’ lives. The present experiment was performed in order to test the hypotheses that self-regulation and mental imagery positively correlate and that mental imagery mediates the correlation of resting heart-rate variability and self-regulation. Self-regulation was measured via a self-report questionnaire (the Short Self-Regulation Questionnaire), as was mental …

Characterizing A Rad23 Dependent Ultraviolet Radiation Resistance In Tetrahymena Thermophila, Emma June Liimatta

Graduate Theses/Dissertations

In 2020, 10 million deaths were attributed to cancer, with multidrug resistance being responsible for over 90% of deaths in cancer patients receiving treatment. This study utilized the model organism Tetrahymena thermophila to study how cells become resistant to Ultraviolet Radiation (UV) radiation, a process similar to multidrug resistance, specifically focusing on the nucleotide excision repair and ubiquitin shuttle protein Rad23. The National Cancer Institute documented 30-60% of cancers tested had a mutation in RAD23. Knockdown of RAD23 in Tetrahymena thermophila demonstrated a UV resistance phenotype with decreased nucleotide excision repair and differential expression of proteins active within caspase-independent …

A Retrieval Augmented Approach To Improving Accuracy Of Biomedical Term Normalization By Large Language Models, Thanh Son Do

Graduate Theses/Dissertations

Ontology normalization is crucial in biomedical text processing, as it enables the mapping of medical expressions to standardized ontology terms and their corresponding identifiers. This thesis explores the feasibility of using large language models (LLMs) for ontology normalization, with a specific focus on the Human Phenotype Ontology and Gene Ontology. Prior research studies indicated that LLMs employing zero-shot learning tend to exhibit low accuracy and are prone to frequent hallucinations. We propose a retrieval augmented generation (RAG) approach to address these limitations and enhance normalization accuracy. We generated synthetic test sets of ontology-derived synonyms to evaluate normalization performance and developed …

Identification Of A Stable Qtl For Juice Color In A Vitis Interspecific Hybrid ‘Chambourcin’- Based Population, Qiuni Yang

Graduate Theses/Dissertations

Berry juice color is one of the key factors influencing grape and wine quality, consumer preference, and marketability. While most previous studies on berry color have focused on the skin, research on juice color remains limited. This study investigated the genetic basis of berry juice color in a hybrid population derived from the Vitis interspecific hybrid ‘Chambourcin’ and V. vinifera ‘Cabernet Sauvignon’. A new nine-category visual scoring system was developed to assess juice color based on OIV 225. To achieve comprehensive precision and quantitative analysis, CIELAB parameters (L*, a*, b*), Hue, and Chroma were extracted from image-based measurements. Using berries …

The Effects Of Calcium Availability On Growth And Calcium Content Of Hydroponic Daikon Radish Microgreens, Cory Michael Reichmuth

Graduate Theses/Dissertations

Microgreens are young, edible seedlings harvested upon emergence of the first true leaf. Their popularity is growing as a nutritionally dense food source that can be grown quickly in space-efficient systems. However, little is known about optimal cultivation practices and the effects of fertilization on growth and nutrition of microgreens. This study investigates the role of calcium (Ca) fertilization in optimizing the growth, yield, and nutrient content of daikon radish (Raphanus sativus) microgreens. A hydroponic system was designed to consistently deliver nutrient solution to microgreens grown in a closed-environment chamber. This study tested different Ca fertilization rates of 0, 5, …

Applied And Conceptual Implications Of Relational Density Theory In Machine Learning And Behavioral Economics, Bentley J. Elliott

Graduate Theses/Dissertations

Relational Density Theory (Belisle & Dixon, 2020a) is an advanced conceptualization of Stimulus Equivalence (Sidman & Tailby, 1982) and Relational Frame Theory (Hayes et al., 2001) that allows behavior scientists to measure the strength, formation, and malleability of covert behavioral experiences such as attitudes, biases, and probabilities. Relational density combines principles of behavior with the fundamentals of Newtonian classical mechanics to quantify the dimensions of relational framing as a tool for examining behavior. This thesis contains two studies on relational density in context. The first study utilized relational density theory and principles of behavioral economics to measure attitudes and charitable …

Relational Density Theory And Intercultural Interaction: Identifying And Shifting Perceptions Of At-Risk Groups, Ryan T. Moser

Graduate Theses/Dissertations

Intercultural interaction is an important aspect for fostering global engagement and competence, yet we find that despite the numerous opportunities, many don’t engage cross-culturally. The current paper utilizes Relational Density Theory (RDT; Belisle & Dixon, 2020a) to examine and modify perceptions of approachability through two experiments. In experiment 1, a Multidimensional Scaling Procedure (MDS) was utilized to identify at-risk groups for non interaction among college students as well as willingness to engage. In experiment 2, a Stimulus Pairing Observation Procedure (SPOP) was utilized to determine whether exposure to positively valanced stimuli could shift the perceptions of the at-risk groups to …

The Effects Of Corporal Punishment In Childhood On Early Adulthood Cortisol And Aggression, Tyler Priest

Graduate Theses/Dissertations

The current study aimed to see if experiencing corporal punishment led to more aggressive behaviors as well as higher cortisol levels than in those who did not experience corporal punishment. The study also examined whether self-regulation moderated the relationship between corporal punishment and levels of aggression. Morning cortisol levels were collected from participants, and they completed a demographics survey, an aggression index, a corporal punishment index, and a self-regulatory inventory index. A T-test found that there was not a significant difference in morning cortisol levels between those that experienced corporal punishment and those that had not experienced corporal punishment. A …

Exploring Power Dynamics In Ghana Through Ceramic Sculpture, Collins Yaw Antwi

Graduate Theses/Dissertations

The works in this thesis explore the implications of power dynamics such as inequality, power struggle, and oppression within Ghana’s society. I abstract specific elements such as coins, and especially cowry shells which honors Ghana’s past through their use as a source of currency in the colonial era and further serves as a metaphor that represents power. Platters and bowls from different cultures serve as a container for these dynamic symbols of power. By incorporating these elements in my sculptures, I bring attention to the struggle and challenges Ghanaians have experienced over the years. In other ways, I find it …

Effects Of Active Learning In An Undergraduate Physiology Class Using Hands-On And Simulation Labs, Ainsley C. Frayer

Graduate Theses/Dissertations

Increasing student engagement has shown positive effects on students and can be achieved by incorporating active learning strategies in the lab. Currently, the undergraduate human physiology lab at Missouri State University utilizes simulations to practice physiological concepts. The proposed project aims to evaluate if incorporating hands-on labs would improve student learning and/or their engagement. The hypothesis is engagement will be increased in hands-on labs, but grades will not differ significantly, based on previous literature. Three simulations were replaced with hands-on lab software to evaluate engagement and learning. Student engagement was assessed using a voluntary, anonymous survey. Exam average scores were …