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Life Without Oxygen: Cellular Mechanisms Enabling Genome Maintenance And Proliferation In Cells From An Anoxia-Tolerant Vertebrate, Riley Asa Roth-Carter

Dissertations and Theses

When exposed to the complete loss of oxygen (anoxia) most organisms undergo rapid cellular deterioration driven by a range of metabolic dysfunctions associated with the loss of the terminal electron acceptor for aerobic respiration. This loss of ability to create and maintain necessary levels of cellular ATP through oxidative phosphorylation contributes to loss of membrane potentials, increase in the accumulation of acidic by-products of anaerobic metabolism, and a decrease in the ability to support other molecular processes that rely on a steady supply of ATP. While this extreme oxygen stress is lethal to a majority of vertebrate organisms there are …

Impact (Immune Monitoring And Phenotype Assessment Of Clinical Trajectory) For Patients With Inborn Errors Of Immunity From The Primary Immune Deficiency Treatment Consortium (Pidtc), Danielle E Arnold, Hesham Eissa, Elizabeth Dunn, Geoffrey D E Cuvelier, Nicola A M Wright, Rebecca A Marsh, Suhag Parikh, Blachy Davila Saldana, Mark T Vander Lugt, Rosa Bacchetta, Lisa Forbes Satter, Shanmuganathan Chandrakasan, Erinn Kellner, Eric J Allenspach, Erica G Schmitt, Brent Logan, Lauri Burroughs, Morton J Cowan, Linda M Griffith, Luigi Notarangelo, Sung-Yun Pai, Michael A Pulsipher, Christopher C Dvorak, Elie Haddad, Jennifer M Puck, Troy R Torgerson, Jennifer W Leiding, Jennifer Heimall, Alice Y Chan

Faculty, Staff and Students Publications

Background: Evaluating natural history and outcomes of rare inborn errors of immunity (IEIs) has been challenging due to the diverse disease manifestations and lack of standardized data.

Objective: To develop a set of standardized, quantitative, generalizable data collection modules to measure organ dysfunction in IEIs, modeled on the previously validated Common Terminology Criteria for Adverse Event system for grading treatment toxicity.

Methods: Disease conditions were organized by organ system. Experts from the Primary Immune Deficiency Treatment Consortium identified essential features of severe combined immunodeficiency, chronic granulomatous disease, Wiskott-Aldrich syndrome, and primary immune regulatory disorders and compared these to Common Terminology …

The Omeract Core Domain Sets For Calcium Pyrophosphate Deposition Disease, Nicola Dalbeth, Yiling Zhang, Owen Hensey, David Grossberg, Ken Cai, Amy Fuller, Geraldine M Mccarthy, Tristan Pascart, Augustin Latourte, Edoardo Cipolletta, William J Taylor, Cesar Diaz-Torne, Tim L Jansen, Lih En Hong, Silvia Sirotti, Fabio Becce, Georgios Filippou, Beverley Shea, Robin Christensen, Jasvinder A Singh, Abhishek Abhishek, Sara K Tedeschi

Faculty, Staff and Students Publications

Objectives: To develop OMERACT core domain sets for chronic and recurrent calcium pyrophosphate deposition (CPPD) disease and acute calcium pyrophosphate (CPP) crystal arthritis.

Methods: Following OMERACT methodology, the CPPD Working Group (comprising of patient research partners, fellows, clinicians, and methodologists) defined the core domain sets. The development of the core sets occurred through a scoping review, qualitative study, Delphi surveys, and ranking exercises to identify the most relevant domains. A virtual e-module was developed to enable members of the OMERACT community to discuss and vote for endorsement of the OMERACT CPPD core domain sets.

Results: The core domains for chronic …

The Relationship Between Psychological Stress And Sars-Cov-2 Nucleocapsid-Antibody Response Following Natural Infection: Longitudinal Findings From The Aegis Study, Erika T Beidelman, Ashley Judge, David B Allison, Stephanie Dickinson, Lilian Golzarri Arroyo, Anna L M Macagno, Michael J Ricciardi, Jon Macy, Stanley Perlman, Christina Ludema, Kevin C Maki, Molly Rosenberg

Faculty, Staff and Students Publications

Psychological stress can cause diminished immune response to infectious challenges. The extent this holds true during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and the modifying effect of vaccination status is untested. We explored these relationships in a non-random sample standardized to the US adult population. Across 2178 participants in the Aegis study (2021-2022), we identified 507 eligible laboratory-confirmed SARS-CoV-2 infections. We estimated the natural log of nucleocapsid-binding antibody absorbance (N-antibody) at study visits up to 225 days following a positive SARS-CoV-2 test. Linear mixed effects models were fit to estimate the association of baseline perceived stress and, separately, …

A Novel Qa Method For Adaptive Aperture Position Verification Of Proton Therapy System, Shuxin Fan, Tiezhi Zhang, Xiandong Zhao, Winter Green, Baozhou Sun, Tianyu Zhao, Yao Hao

Faculty, Staff and Students Publications

Accurate Adaptive Aperture (AA) leaf positioning is essential for some proton therapy systems to ensure precise dose delivery and patient safety. However, conventional quality assurance (QA) methods can be suboptimal or inefficient for routinely assessing the dynamic nature of AA in the Mevion S250i HYPERSCAN system. This study introduces a novel automated QA method to verify AA positions utilizing a scintillation detector. A specialized QA plan consisting of 7 rectangular fields was generated in the RayStation treatment planning system (TPS) and delivered using the Mevion S250i HYPERSCAN proton therapy system. Dose distributions were measured with an IBA Lynx PT scintillation …

Complement Activation In Maternal And Placental Pathology Of Preeclampsia, Manu T Banadakoppa, Madhulatha S Chauhan, Moises J Tacam, Allyson W Nevins, Antonio H Ruano, Simone H Ruano, Jon A Fuson, Chandra Yallampalli

Faculty, Staff and Students Publications

Background: Preeclampsia is a multifactorial, pregnancy-related disorder characterized by new-onset hypertension and proteinuria, with distinct early- and late-onset forms. Although complement dysregulation has been implicated in the pathogenesis of preeclampsia, its causal role remains unclear. In mice, Crry (complement receptor 1-related protein Y) functions as a critical complement regulator at the fetal-maternal interface. Because complete Crry deficiency is embryonically lethal, direct in vivo investigation of complement activation in pregnancy has been challenging.

Methods: We generated a placenta-specific, doxycycline-inducible short hairpin RNA mouse model in which Crry expression can be downregulated in a dose-dependent manner. This system employs Cyp19-driven Cre recombinase …

Cytosine Base Editing Of Lpa In Transgenic Mice Averts Large Deletions, Marcel A Chuecos, So Hyun Park, Madhvi M Bhakta, Usosa Too-Chiobi, Daniel Betancourth, Mingming Cao, Marco De Giorgi, Christopher J Walkey, Anjana Tiwari, Biana Godin, Julia M Assini, Donna J Palmer, Philip Ng, Michael B Boffa, Marlys L Koschinsky, Gang Bao, William R Lagor

Faculty, Staff and Students Publications

Lipoprotein(a) (Lp(a)) is a genetically determined causal risk factor for cardiovascular disease, with approximately 20% of the population exhibiting elevated levels. While there are promising drugs in development, there are currently no approved therapies specifically designed to lower Lp(a) levels. For high-risk individuals with extreme levels of Lp(a), liver-directed genome editing could be an effective one-time solution. Genome editing approaches such as CRISPR and TALENs can reduce Lp(a) in LPA-transgenic mouse models, but they frequently induce large and potentially harmful genomic deletions. Here, we report the first application of TadA-derived cytosine base editing (CBE), delivered via helper-dependent adenovirus (HDAdV) and …

Transgenic Hookworm Secretes Anti-Tetrodotoxin Human Single Chain Antibody, Kumar Sachin Singh, Suman Bharti, Bruce A Rosa, Mahdiyeh Bigham, Samuel C Uzoechi, Young-Jun Choi, John C Martin, Danielle Kemper, Slavica Pavlovic Djuranovic, Darren A Pickering, Rachael Ryan, Bethany K Bracken, Maria Elena Bottazzi, Eric C Carnes, Wannaporn Ittiprasert, Matthew Moyle, Paul J Brindley, Alex Loukas, Sergej Djuranovic, Makedonka Mitreva

Faculty, Staff and Students Publications

Biologics, protein- and peptide-based drugs derived from living organisms or cell lines have emerged as effective therapies across a broad range of indications. However, parenteral administration and the need for frequent dosing increase costs and limit compliance, creating an urgent need for innovative platforms capable of continuously, safely, and efficiently delivering sustained biologics in situ within the host. Here, we describe a bioengineered hookworm platform to manufacture and deliver biologic therapeutics in vivo. As proof of concept, we engineer the Ancylostoma ceylanicum secretome by inserting a human single-chain variable fragment antibody (s16-HuScFv) into its genome. Transgene expression does not perturb …

Defining The Dynamic Role Of Belongingness In Alcohol Use Disorder Treatment, Brendan E. Walsh

USF Tampa Graduate Theses and Dissertations

Drinking is intertwined with social processes, including social support and group belongingness. Although drinking can transiently enhance social functioning, escalating to heavy use can be detrimental to it. It is well-known that excessive drinking and social dysfunction exacerbate each other over time, and it follows that social support and belonging are key mechanisms of addiction recovery. Yet, less is known about how changes in belongingness throughout treatment dynamically affect drinking outcomes, and even less is known about how drinking outcomes and other social processes (e.g., therapeutic alliance, interpersonal drinking consequences) dynamically influence belongingness. The current project (N = 210) studied …

A Comprehensive Survey Of Artificial Intelligence Applications In Predicting Mining-Induced Subsidence, Deformation, And Landslides: Strengths, Limitations, And Future Trends, Long Quoc Nguyen, Dung Ba Nguyen, Minh Tuyet Dang

Journal of Sustainable Mining

Mining activities often cause mining-induced ground deformation, including subsidence and landslides, and related geo-environmental impacts, posing significant risks to infrastructure and safety. This study conducts a systematic assessment to identify, categorize, and evaluate AI-based methods (machine learning, deep learning, and hybrid models) for predicting and monitoring mining-induced ground deformation. The literature search was performed across major scientific databases, using predefined keywords and selection criteria, resulting in a final dataset of relevant peer-reviewed studies. The reviewed works were classified into three methodological groups: traditional machine learning, deep learning-based approaches, and hybrid methods. The results show that ML still dominates in terms …

The Constitutionality Of The Special Trial Counsel Under The Apogee Of Judicial Deference, Josef Danczuk

Pace Law Review

Congress has undertaken some of its broadest reforms of military courts-martial in decades. The creation of the Special Trial Counsels (STCs) intentionally removes nearly all courts-martial powers from military commanders for “covered offenses,” some of the most serious prosecutions. Many commentators have expressed concern that this change untethers the courts-martial system from its historical anchor of discipline within the military—an anchor that the Supreme Court has used to grant exceptional deference to Congress for its legislation pursuant to the Make Rules Clause. However, the constitutionality of the STCs and the deference federal courts grant Congress in this realm should not …

Personas Issue 4, 2026, Jonathan Maiullo

Personas: Multilingual Creative Writing Journal

No abstract provided.

Jubilación, Janet Munoz

Writing Waves

No abstract provided.

Daydreaming: How Far Can We Go?, Abigail Toribio

Writing Waves

No abstract provided.

The War For American Independence Was Biblical And Just*, Mark David Hall

Journal of Law and Government

In his essay, Mark David Hall presents an overview of Christian scholarship on the question of whether the War for American Independence is biblical and just. He then considers Romans 13 and shows that Protestants, especially Calvinists, came to interpret these verses to permit active resistance to tyrannical rulers. Finally, he discusses the conflict in light of the just war tradition and contends that the patriots had excellent reasons to believe their active resistance was biblical and just.

Was The American Revolution Biblical And/Or Just?, Gregg L. Frazer

Journal of Law and Government

The questions of whether the American Revolution was biblical and whether it was just may be necessarily intertwined. If it was biblical, then it was just. If it was unbiblical, it was necessarily unjust. If it was merely non-biblical, that is, if the Bible does not speak to the question, then it could be either just or unjust by some human standard. For the purposes of this paper, the question of whether it was just by a merely human standard will be addressed independently of, and in addition to, the infinitely more important question of whether it is biblical. We …

Introducing The Covenantal-Noncentralization Index (Cni) For Assessing Localism, Social Capital, Community Challenges And Community Health, Kahlib Fischer, Edwin Christmann, Melissa Stewart, Todd Robatin, Zachary Federico, John Kebles, Lucy Lambert, Alex Mishra

Journal of Law and Government

Numerous indices measuring social capital and community challenges at the local level exist, but none attempt to offer a measure of community health as an independent variable in comparison to community challenges as a dependent variable. Therefore, we propose the Covenantal-Noncentralization Index (CNI) in an effort to provide a more nuanced understanding of social capital at the local level as well as to differentiate between an independent variable index consisting of family stability, religious adherence, non-profit activity, and charitable giving and dependent variables representing various community challenges: violent crime, childhood poverty, STD rate, high school diploma completion, GDP, and unemployment. …

Defending The West By Reclaiming The Past, Steven Alan Samson

Journal of Law and Government

The Western world was originally shaped by the fine arts and philosophy of Greece, the architecture and law of Rome, and the faith, morality, and prophetic traditions of Judaism and Christianity. As the West loses the religious bond that generated its culture, careful attention should be given to reclaiming and rebuilding this moral and spiritual infrastructure.

Acknowledgements, Editors Of Suurj

SUURJ: Seattle University Undergraduate Research Journal

No abstract provided.

Toward Liberation: Decolonizing Curriculum To Heal Double Consciousness In Students Of Color, Olivia Cimino

SUURJ: Seattle University Undergraduate Research Journal

This paper explores how education can be reimagined as a form of liberation through the integration of interdisciplinary theories from political science, ethnic studies, and educational studies. Grounded in Antonio Gramsci’s theory of cultural hegemony and Louis Althusser’s concept of the Ideological State Apparatus, this paper introduces the concept of Reverse Hegemony as a framework to reclaim educational curriculum and mitigate systemic injustice. By applying this framework alongside liberatory pedagogies informed by Paulo Freire, Jarvis Givens, and Bryan Brayboy, the paper argues that education can heal the fractured sense of identity that W.E.B. Du Bois termed double consciousness. Through interdisciplinary …

Contributor Biographies, Editors Of Suurj

SUURJ: Seattle University Undergraduate Research Journal

No abstract provided.

Gentrification As A Consequence Of Light Rail Development In Rainier Valley, Seattle, A Policy Analysis, Jo C. Moreau

SUURJ: Seattle University Undergraduate Research Journal

This policy analysis examines the gentrification and displacement impacts resulting from the development of Seattle’s Link Light Rail in the Rainier Valley, one of the city’s most diverse and historically marginalized neighborhoods. The expansion of light rail aimed to improve transit access, reduce traffic congestion, and promote environmental sustainability. However, coinciding market driven development, rising property values, and demographic shifts appear to disproportionately affect low-income and BIPOC (Black, Indigenous, and People of Color) residents, making them more vulnerable to displacement (Padeiro et al., 2019). This report will evaluate three policy interventions to mitigate displacement: the Mandatory Housing Affordability (MHA) program, …

Between The Canvas And The Colonizer: The Black Female Figure And French Orientalist Paintings, Sophia Hounton

SUURJ: Seattle University Undergraduate Research Journal

Black women in French Orientalist paintings have been portrayed as hypersexual, subservient, and used as props to highlight the presence of their white and Arab counterparts. These depictions of Black women represent early discriminatory ideas about Black Africans that emerged in the same era through racial science. Though partially deconstructed today, these early conceptions of Black women continue to be reinforced and impact modern perceptions of Black women in French society. There is notably much less research dedicated to the historical role of the image of Black women in France compared to research on Black men and Arab women. This …

The Child Figure In Climate Fiction: Redefining Community And Culture In The Face Of Climate Change, Allison Wasley

SUURJ: Seattle University Undergraduate Research Journal

While climate change has been a known phenomenon for decades, social and cultural factors have stagnated our collective ability not only to discuss ongoing climate disasters, but also to conceptualize the disintegration of the very relationship between humanity and the natural world. This essay builds upon existing research and theory, proposing that climate fiction could serve as an antidote to our “cultural derangement,” which simultaneously destroys the relationship between humanity and nature, and our relationship with one another. This essay analyzes several examples of climate fiction that leverage the figure of the child as a symbol of hope and a …

Creating Accessible And Equitable Mental Health Treatments: The Usefulness Of Psychedelic-Assisted Therapy In Treating Racial Trauma, Aicha J. Toure

SUURJ: Seattle University Undergraduate Research Journal

Psychedelic-assisted therapy is a recently developed method for treating mental health disorders, including anxiety, depression, and post-traumatic stress disorder (PTSD). Research into the effectiveness of psychedelics in treating mental health has largely excluded Black Peoples, Indigenous Peoples, and People(s) of Color (BIPOC); this is problematic, as rates of these mental health disorders are proportionally higher in BIPOC individuals than in white individuals. Mental health issues experienced by BIPOC are, more often than not, rooted in experiences of racial discrimination (Schouler-Ocak et al., 2021). Experiences of racial discrimination can develop into racial trauma, manifesting as depression, anxiety, and/or PTSD. This literature …

School-To-Prison Pipe Dream, Kadeen C. Frazier

SUURJ: Seattle University Undergraduate Research Journal

The school-to-prison pipeline is a disturbing reality that is one of the most grotesque and ironic manifestations of systemic failure. This phrase, school-to-prison pipeline, is used to describe the national trend where children almost exclusively from marginalized communities are funneled out of public school and into the juvenile and criminal justice system. The United States public education system pushes its most vulnerable citizens, children, from an institution where they are sent to safely learn and grow, into one that is like hell on Earth, where they will be stripped of their humanity and work as slave labor in privately owned …

Queer (Re)Formations Of Mexican Identity: Modernity & Visibility As Seen In El Vampiro De La Colonia Roma, Y Tu Mamá También, And Sueño En Otro Idioma, Mar Bradley

SUURJ: Seattle University Undergraduate Research Journal

Starting with the infamous Ball of the 41, the perception of Mexican male queerness has been strongly affected by its visibility. The Ball of the 41 attracted massive attention from the press; popular engraver José Guadalupe Posada completed two prints on the ball, mocking the attendees. For the first time in modern Mexico, queerness was represented in widespread public media, as knowledge of the scandal disseminated throughout the country by articles and images printed in newspapers. A politics of visibility is central to understanding the representational shifts of queer people, particularly by studying how queer people represent or are represented …

Suurj Volume 10 Entire Volume, Editors Of Suurj

SUURJ: Seattle University Undergraduate Research Journal

No abstract provided.

Complete Issue, Dawson A. Phelps, Edward Hunter Ross, Owen M. Peterson, Paul Flowers

Journal of Mississippi History

No abstract provided.

Names Please: Place Names Along The Natchez Trace, Dawson A. Phelps, Edward Hunter Ross

Journal of Mississippi History

No abstract provided.