Digital Commons Network^™

Genetic Engineering Of Esophageal Organoids: Crispr-Based Knock-In For Cell Lineage Tracing, Kyung-Pil Ko, Jae-Il Park

Faculty, Staff and Student Publications

Esophageal organoids serve as powerful systems to study epithelial lineage hierarchies and cancer biology. Gene manipulation in these organoids has traditionally involved overexpression or knockout strategies. However, CRISPR-/Cas9-based knock-in (KI) approaches now enable precise cell lineage tracing and live imaging. Here, we describe protocols to generate fluorescent KI organoids from murine esophageal epithelium by tagging Krt13 (BFP) and Sox2 (mNeon). These dual-reporter organoids allow direct monitoring of growth dynamics and differentiation trajectories. We outline CRISPR/Cas9 design, donor construction using homology-independent approaches (CRISPaint), delivery into organoid cells, enrichment and single-clone isolation, and validation by fluorescence. For organoids, homology-directed repair (HDR) can …

"What's A Computer?" Exploring Insurance Agents' Role In Small Business Cyber Insurance Transactions, Dalton A. Crabtree

USF Tampa Graduate Theses and Dissertations

This mixed-methods study examined how insurance agents' role identity impacts cyber insurance transactions, addressing gaps in professional practice and academic literature. Small businesses increasingly rely on cyber insurance for protection as cyber risk continues to evolve. The study employed sequential explanatory methodology with 25 interviews across three groups: insurance agents (n=15), corporate cyber insurance professionals (n=5), and small business owners (n=5). Thematic analysis using Katz and Khan's Role Episode Model informed development of the Cyber Insurance Role Identity Scale (CIRIS), tested with 100 agents. Six themes emerged. Key findings reveal that successful cyber insurance transactions require agents to transition from …

Establishing Priority Pediatric Antimicrobial Stewardship Interventions In The Us: Findings From A Delphi Consensus Study, Harry Obeng, Emmanuel Tetteh, Sara Malone, Lauren Walsh, Tyler Walsh, Fernando J Bula-Rudas, Ritu Banerjee, Adam W Brothers, Joshua C Herigon, Katie Namtu, Scott Weissman, Daniel Riggsbee, Jared Olson, Debra Lynn Palazzi, Ann Wirtz, Matthew Sattler, Jessica Tansmore, Brittany A Rodriguez, Monica Abdelnour, Joshua R Watson, Alison C Tribble, Jessica Gillon, Mari Nakamura, Sarah Jones, Jason G Newland, Virginia R Mckay

Faculty, Staff and Students Publications

Background/objectives: Antimicrobial resistance (AMR) is a major global health threat, with children at higher risk due to developmental differences in drug metabolism, limited treatment options and inappropriate antibiotic use. Pediatric antimicrobial stewardship programs (ASPs) face implementation challenges, often relying on adult-based guidelines and limited pediatric-specific evidence. This study aimed to identify and prioritize the most critical areas for pediatric ASP intervention development through a structured, multi-round Delphi consensus process with experts in antimicrobial stewardship and infectious diseases.

Method: A four-round modified Delphi process was conducted to identify and prioritize key pediatric ASP interventions. Experts in antimicrobial stewardship and infectious diseases …

Artificial Intelligence In Education: The Power And Dangers Of Chatgpt In The Classroom, Muhammed Parviz

Journal of Educational Research and Practice

The integration of artificial intelligence (AI) in education has accelerated significantly in recent years, fundamentally shaping pedagogical practices and learning experiences. Among these advancements, OpenAI’s ChatGPT stands out as a sophisticated language model capable of generating human-like text. This capability opens new avenues for educational applications, such as personalized learning experiences and automated assessments. By embedding ChatGPT into e-learning platforms, educators can provide instantaneous feedback and foster interactive language learning. However, the deployment of ChatGPT in educational contexts presents challenges, particularly ethical issues related to data privacy and the potential for inherent biases in AI models.

In this context, the …

Surprises From The Shadows: Confronting Intrusions From Bots & Generative Ai In Survey Research, Vincent Cho, Sofia I. Duenas

Journal of Educational Research and Practice

Unbeknownst to many researchers, online surveys can be vulnerable to attacks from bots and generative artificial intelligence (AI), which can generate hundreds of fraudulent responses instantly. Confronting this challenge to data integrity, we employed an adaptive approach to test the effectiveness of various anti-fraud tactics (e.g., CAPTCHA; honeypot questions; question pairs; open-ended questions) and to distinguish between good-faith human respondents versus bots or fraudsters. Findings revealed that bots with generative AI capabilities bypassed some conventional defenses, including CAPTCHA, and effectively supplied human-like responses to open-ended questions. Other tactics, like removing response incentives and verifying IP addresses, showed promise but came …

Teaching Medical English To Non-Native Speakers Involved In Healthcare: A Review Of Challenges, Practical Solutions, And Implications, Elisa Nsanzimana

Journal Of Indian Physician Associates

Background Teaching medical English to non-native speakers presents challenges that go beyond standard English language instruction. These include specialized medical vocabulary, complex syntax, and the crucial role of culturally appropriate communication in healthcare settings. Such factors can negatively impact learning outcomes and the quality of patient care.

Objective This review aims to identify the key challenges faced by non-native speakers in learning medical English and explore practical, evidence-based solutions to address these obstacles.

Methodology This is a narrative review conducted to review the various literature concerning medical English teaching for non-native speakers. PubMed, Google Scholar, and Web of Science were …

Revolutionizing Digital Health In Rwanda: Progress Toward Universal Health Coverage, Roméo Bananeza Mr

Journal Of Indian Physician Associates

Rwanda is an African country that is making rapid progress in all sectors, including digital health. This review explores Rwanda’s transformative journey in digital health, highlighting key achievements, ongoing challenges, community involvement, and future prospects. Rwanda’s commitment is evident in using electronic health records, telemedicine, and mobile health applications. Rwandan institutions, including Rwanda Biomedical Center, Rwanda Ministry of Health, Rwanda Health Information Exchange, Rwanda Development Board, Partners in Health, universities, and several nongovernmental organizations, have made significant contributions to revolutionize digital health in Rwanda. However, the country continues to face barriers, including limited digital infrastructure, financial constraints, and disparities in …

Women Of The Fairy Tale Resistance: The Forgotten Founding Mothers Of The Fairy Tale And The Stories That They Spun By Jane Harrington, Rebecca Mccurdy

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

This article gives a review of Jane Harrington's Women of the Fairy Tale Resistance: The Forgotten Founding Mothers of the Fairy Tale and the Stories That They Spun. The review highlights the book as a missed opportunity due to improper citations, an odd tone, and a confused view of who the intended reader is.

Speculative Poetry And The Modern Alliterative Revival: A Critical Anthology, Edited By Dennis Wilson Wise, Douglas A. Anderson

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Book review, by Douglas A. Anderson, of Speculative Poetry and the Modern Alliterative Revival: A Critical Anthology (2024), edited by Dennis Wilson Wise

Nordic Sagas As Children’S Literature: Victorian And Edwardian Retellings In Words And Pictures By Velma Bourgeois Richmond, Maria K. Alberto

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

No abstract provided.

Memory And Medievalism In George R.R. Martin And Game Of Thrones: The Keeper Of All Our Memories, Edited By Anna Czarnowus And Carolyne Larrington, Kris Swank

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

This review of Memory and Medievalism in George R.R. Martin and Game of Thrones, edited by Anna Czarnowus and Carolyne Larrington, evaluates the collection’s contribution to medievalist scholarship on Martin’s works. It highlights the volume’s thematic breadth—from historical reimaginings and institutional critiques to emotional theory and portrayals of violence—while emphasizing the fresh perspectives offered by European non-Anglophone scholars. The reviewer praises the essays’ nuanced engagement with medieval traditions and their relevance to Martin’s narrative choices in both the books and the HBO adaptation. Ultimately, the review affirms the collection’s valuable contribution to the study of Martin’s medievalism.

Historical Dictionary Of Horror Literature, Edited By Mark A. Fabrizi, Douglas A. Anderson

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Book review of Historical Dictionary of Horror Literature (2023) by Mark A. Fabrizi

Once Upon A Place: Forests, Caverns & Other Places Of Transformation In Myths, Fairy Tales & Film By Holly Bellebuono, Isabella Martins-Simonsen

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

No abstract provided.

Germanic Heroes, Courage, And Fate: Northern Narratives Of J.R.R. Tolkien’S Legendarium By Richard Gallant, Cami Agan

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

No abstract provided.

C.S. Lewis On Literature: An Introduction To His Literary Criticism, Literary History, Literary Theory By Marcus K. Paul, James Hamby

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

No abstract provided.

Owen Barfield’S Poetry, Drama, And Fiction: Rider On Pegasus By Jeffrey Hipolito, Landon B. Loftin

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

No abstract provided.

But I Made You Look: Mysticism, Desire, And The Refusal Of The Literal, Züleyha Çetiner-Öktem

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

In response to the review of her edited collection in Mythlore #142, the author elucidates her decision to provide only a vague description of a controversial piece of art.

Tolkien’S Tune For “Namárië” Is An Actual Gregorian Chant, Nancy Martsch

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

In response to Tom Emanuel’s essay on the Tolkien Society’s Enyalië ceremony (Mythlore #146), notes the origin of Tolkien’s tune for the poem.

God’S Vow: A Note On C.S. Lewis’S Idea Of Human Freewill And Divine Omnipotence, Matthew Mckenna

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

This note uses G.K. Chesterton’s understanding of vows as a hermeneutical key for a line from C.S. Lewis’ Miracles where Lewis says that God relinquishes part of his power by creating freewill. Chesterton’s idea of vows sees them as acts of power and freedom which paradoxically limit one’s freedom in the future by compelling one to keep one’s vow. The vow is an act of freedom which relinquishes one’s freedom by committing one to certain actions. Viewed through this lens, God’s creation of freewill is seen as an act of power and freedom.

Tolkien’S Exceptional Visit To Holland, Revisited: An Acquaintance With Maartje Draak, Bart Jaski, Kris Swank

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

This note revisits J.R.R. Tolkien’s trip to the Netherlands, March 28-31, 1958, including his attendance at the Rotterdam Hobbit-maaltijd (“Hobbit Meal” or “Hobbit Dinner”), and afterwards. It focuses specifically on his interactions with Prof. Dr. Maartje Draak, a prominent Dutch Celticist. Although Prof. Draak is not mentioned in René van Rossenberg’s paper, “Tolkien’s Exceptional Visit to Holland: A Reconstruction” (Mythlore 1996), items from Prof. Draak’s archives at Utrecht University and an offprint paper she sent to Tolkien in 1959 allow us to recover Tolkien’s acquaintance with this important Dutch scholar, to enhance our picture of the famous Hobbit Dinner, …

Credit Where Credit’S Due: Douglass Parker And J.S. Ryan, Matthew Thompson-Handell

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

This Note presents evidence that J.S. Ryan's 1966 essay on Germanic influences on Tolkien plagiarized some content in Douglass Parker's 1957 review essay on The Lord of the Rings "Hwaet, We Holbytla."

Tolkien's Invasion Of Dragons, Ian Gunn

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Throughout his life, Tolkien’s works demonstrate a particular interest in the heroic legendary past and how the memory of such tales can enlighten and vivify society. In his own writings on dragons, adventures, and legendary tales (both historical and fictional), Tolkien participates in the restoration of our memories of the high and the perilous.

The Soldier’S Stigmata, Part I: Habitus And Speaking Of The Future Otherwise In Lois Mcmaster Bujold’S “The Borders Of Infinity”, Andrew Hallam

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

THIS IS A TWO-PART ARTICLE. IN ORDER TO READ OR DOWNLOAD THE OTHER PART, FOLLOW THE LINK AT THE END OF THIS ABSTRACT.

This article will explore the connection between Lois McMaster Bujold’s novella “The Borders of Infinity,” the Aristotelian theory of habitus as developed further in the letters of the Apostle Paul and by the sociologist Pierre Bourdieu, and the four senses of interpretation of scripture first conceived in antiquity and later refined by medieval philosophers. Habitus as a durable disposition, state, or condition (Aristotle) is a structured and structuring social practice or set of social practices (Bourdieu) that …

Eleanor Arnason’S Fantastic Mitochondrial Humor, Sandra J. Lindow

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Eleanor Arnason’s Fantastic Mitochondrial Humor

In this essay I explore Brian Attebery’s description of mitochondrial fantasy to explain how humor works in Eleanor Arnason’s short fantastic fiction. Mitochondria are the energy powerhouses in cells. They are symbiotic creatures that once were separate but became an evolutionary part of a female egg cell, providing a history that can be traced to a Mitochondrial Eve. In Fantasy: How It Works, Attebery uses mitochondria metaphorically to explain how fantastic ideas evolve. Attebery describes fantastic mitochondria as “a cellular dynamo that lives within the tissues” of a story (91). He demonstrates how stories lead …

Literary Tributaries: Classical And Romantic Influences In The Wind In The Willows, Landon Burgess Loftin

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Some interpreters of The Wind in the Willows have suggested that Ratty’s beloved river should be understood as a symbol of the imagination. Whether this is true or not, we may benefit from thinking of Grahame’s own imagination as a river that derives much of its force from both Classical and Romantic tributaries. In what follows, I will explore the lower reaches of these tributaries, not only to contextualize and illuminate The Wind in the Willows, but also to address several persistent questions about its composition and reception. I turn first to the Classical tributary, which flows with the imaginings …

Saunders Lewis, J.R.R. Tolkien, And The Place Of Magic In Fantasy, Alexander Retakh

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Welsh writer Saunders Lewis was, just as his almost exact contemporary J.R.R.Tolkien, a politically conservative Catholic with a deep interest in medieval myths. Both authors wrote fantasy based on Celtic tales, such as Lewis's play Blodeuwedd and Tolkien's poem The Lay of Aotrou and Itroun. While the latter follows the dicta of Tolkien's own "On Fairy-stories," Lewis created a more modernist work. Despite biographical and ideological similarities, the two approached their sources differently. Tolkien looked for unifying aesthetic principles that he called "unhazy unromantic momentariness," while Lewis used the traditional material to escape the constraints of romantic and realistic …

Fathoming The Mathom-House: Museums And Material Heritage In Tolkien’S Legendarium, Stuart Bowes

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

As a renowned author and philologist, J.R.R. Tolkien has long been associated with the processes of literary transmission performed by libraries and archives. His engagement with the comparable cultural institution of the museum is less evident. By charting the gradual integration of the Michel Delving Mathom-house into Middle-Earth, this paper considers Tolkien’s fictional interpretation of a contemporary museum and how it served to enrich his legendarium. It first traces the textual history of this collecting institution from its marginal addition in the drafts of The Hobbit to its considered treatment in The Lord of the Rings. A pivotal development …

Lothlórien: Gendered Spaces, Cartographic Nominalism, And Galadriel’S Queerness, Bo Kampmann Walther

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Tolkien’s portrayal of Lothlórien is layered with complexities that reflect broader tensions between the feminine landscape and the masculine impulse to understand, name, and rationalize the world. This article examines the ‘fight’ over Lothlórien and its entitlement to truth from both female and male perspectives. It combines a close reading of passages from The Fellowship of the Ring with theories of name-giving, power, and mapping. The article concludes by evaluating these insights through the lens of Kristeva’s concept of the “abject” and discussing what ‘queer’ might mean in the Tolkien oeuvre.

Tolkien On Kennings And The River-(Woman’S) Daughter, Derek Simon

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

THIS IS A TWO-PART ARTICLE. IN ORDER TO READ OR DOWNLOAD THE OTHER PART, FOLLOW THE LINK AT THE END OF THIS ABSTRACT.

While the River-daughter is a prevalent poetic name for Goldberry, Tom introduces her as the River-woman’s daughter when singing and skipping along the banks of the Withywindle. The River-woman variant occurs only once in Goldberry’s narrative but is not easily dismissed. The contrast between the two variant names raises several questions on how to read Goldberry’s enigma. Tolkien’s brief commentaries on the kenning as a poetic device serve as the starting-point for exploring the literary function of …

Unknowing In Tolkien's Legendarium And The Agnosia Of The Elvish Tradition, Benjamin Blakeslee-Drain

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

The many layers of Unknowing in Tolkien’s legendarium make some of the most profound statements in his work. Unknowing creates space for the reader to have a more active and intimate experience with the text, and supports the central Christian moral message in The Lord of the Rings. The theme of Unknowing ties the legendarium to a larger set of scriptural and Christian writings, including the Book of Job, Mystical Theology, and The Cloud of Unknowing. Finally, Unknowing is shown to be a function of the legendarium reflecting a dominantly Elvish perspective. By dismissing the assumed authority of that perspective, …