Digital Commons Network^™

Usage Statistics: Project Counter R5 Tr_J1 Report Fy2025 - Journal Requests (Controlled) By Title, Andrée Rathemacher

Technical Services Reports and Statistics

Project COUNTER R5 / 5.1 Report TR_J1 for the University of Rhode Island for the period from July 1, 2024 - June 30, 2025. The TR_J1 report is defined as "Journal Requests (Controlled)." This report presents an annual total only and only includes those platforms successfully configured for automated harvesting via SUSHI.

File for download is Excel spreadsheet generated by Alma Analytics.

Results:

Total Item Requests - 850,324

Unique Item Requests - 628,581

Phase 1b/2 Study Of Bms-813160, A Ccr2/5 Dual Antagonist, In Combination With Chemotherapy Or Nivolumab In Patients With Advanced Pancreatic Or Colorectal Cancer, Dung T. Le, Gunnar Folprecht, Anna M. Varghese, Martin Gutierrez, Marcus Noel, Nikolaos A. Trikalinos, Eric Chen, Farshid Dayyani, S. Lindsey Davis, Wen Wee Ma, Atrayee Basu Mallick, Ignacio Garrido-Laguna, Mayu Osawa, Shaun O'Brien, Ruslan D. Novosiadly, Ke Xu, Danielle M. Greenawalt, Santanu Dutta, Christina Twyman Saint Victor, Heinz-Josef Lenz

Department of Medical Oncology Faculty Papers

BACKGROUND: Cysteine-cysteine chemokine receptors 2 (CCR2) and 5 (CCR5) contribute to immune suppression in tumor microenvironments. CCR2 and CCR5 antagonists have demonstrated antitumor activity in pancreatic ductal adenocarcinoma (PDAC) and colorectal cancer (CRC), respectively. This phase 1b/2, open-label study evaluated BMS-813160, a CCR2/5 dual antagonist, in combination with chemotherapy±nivolumab in advanced PDAC or metastatic CRC.

METHODS: Part 1 included patients with metastatic untreated (first-line (1L)) PDAC, 1L CRC, or previously treated (second or third line (2/3L)) microsatellite stable (MSS) CRC. Patients received 2 weeks of BMS-813160 monotherapy (300 mg two times a day, 600 mg once daily, 300 mg once …

Usage Statistics: Project Counter R5 Dr_D1 Report Fy2025 - Database Search And Item Usage, Andrée Rathemacher

Technical Services Reports and Statistics

Project COUNTER R5 / R5.1 Report DR_D1 for the University of Rhode Island for the period from July 1, 2024 - June 30, 2025. The DR_D1 report is defined as "Database Search and Item Usage." This report presents an annual total only and only includes those platforms successfully configured for automated harvesting via SUSHI.

File for download is Excel spreadsheet generated by Alma Analytics.

Results:

Searches Automated - 38,089

Searches Federated - 1,810

Searches Regular - 1,304,882

Total Item Investigations - 933,864

Total Item Requests - 286,912

Usage Statistics: Project Counter R5 Ir_M1 Report Fy2025 - Multimedia Item Requests, Andrée Rathemacher

Technical Services Reports and Statistics

Project COUNTER R5 / 5.1 Report IR_M1 for the University of Rhode Island for the period from July 1, 2024 - June 30, 2025. The IR_M1 report is defined as "Multimedia Item Requests." This report presents an annual total only and only includes those platforms successfully configured for automated harvesting via SUSHI.

File for download is Excel spreadsheet generated by Alma Analytics.

Results:

Total Item Requests - 3,357

Usage Statistics: Project Counter R5 Pr_P1 Report Fy2025 - Platform Usage, Andrée Rathemacher

Technical Services Reports and Statistics

Project COUNTER R5 / 5.1 Report PR_P1 for the University of Rhode Island for the period from July 1, 2024 - June 30, 2025. The PR_P1 report is defined as "Platform Usage." This report presents an annual total only and only includes those platforms successfully configured for automated harvesting via SUSHI.

File for download is Excel spreadsheet generated by Alma Analytics.

Results:

Searches Platform - 294,352

Total Item Requests - 1,451,061

Unique Item Requests - 1,078,034

Unique Title Requests - 55,776

Usage Statistics: Project Counter R5 Tr_B1 Report Fy2025 - Book Requests Controlled) By Title, Andrée Rathemacher

Technical Services Reports and Statistics

Project COUNTER R5 / 5.1 Report TR_B1 for the University of Rhode Island for the period from July 1, 2024 - June 30, 2025. The TR_B1 report is defined as "Book Requests Controlled)." This report presents an annual total only and only includes those platforms successfully configured for automated harvesting via SUSHI.

File for download is Excel spreadsheet generated by Alma Analytics.

Results:

Total Item Requests - 194,197

Unique Title Requests - 60,001

Usage Statistics: Project Counter R5 Tr_B1 Report Fy2025 - Book Requests (Controlled) By Platform, Andrée Rathemacher

Technical Services Reports and Statistics

Project COUNTER R5 / 5.1 Report TR_B1 for the University of Rhode Island for the period from July 1, 2024 - June 30, 2025. The TR_B1 report is defined as "Book Requests Controlled)." This report presents an annual total only and only includes those platforms successfully configured for automated harvesting via SUSHI.

File for download is Excel spreadsheet generated by Alma Analytics.

Results:

Total Item Requests - 194,197

Unique Title Requests - 60,001

Usage Statistics: Project Counter R5 Tr_J1 Report Fy2025 - Journal Requests Controlled) By Platform, Andrée Rathemacher

Technical Services Reports and Statistics

Project COUNTER R5 / 5.1 Report TR_J1 for the University of Rhode Island for the period from July 1, 2024 - June 30, 2025. The TR_J1 report is defined as "Journal Requests Controlled)." This report presents an annual total only and only includes those platforms successfully configured for automated harvesting via SUSHI.

File for download is Excel spreadsheet generated by Alma Analytics.

Results:

Total Item Requests - 850,324

Unique Item Requests - 628,581

The Cowl - V. 89 N. 11 - January 22, 2026

The Cowl

The Cowl - student newspaper of Providence College. Vol. 89 No. 11 - January 22, 2026. 20 pages.

Implementing Emotional Intelligence To Enhance Leadership Abilities In A Skilled Nursing Home, Nkechi Amaechina

Walden Dissertations and Doctoral Studies

To meet dynamic changes in healthcare, effective leadership is necessary. A major problem with effective leadership is the lack of emotional intelligence (EI). The purpose of this integrative review is to identify strategies that enhance skilled nursing home leadership abilities using EI. The Leader-Member Exchange (LMX) theory was used, supported by Complex Adaptive Systems (CAS)Theory. LMX theory explains that leaders-members relationships affect an organization while CAS theory describes healthcare industry as ever-changing. The review question (RQ) that guided this integrative review is: what best EI strategies can be implemented to enhance leadership abilities in a skilled nursing home? Twenty-six articles …

Determining The Sources Of Aerosols Delivered To The Subtropical North Atlantic Using Stable Isotopes, Khloe E. Coffie, Zach B. Bunnell, Dylan J. Halbeisen, Peter N. Sedwick, Tim M. Conway

Making Waves REU

Atmospheric deposition of aerosols is a source of bioavailable trace metals1.

• Fe - Required by primary producers for photosynthesis and nitrogen fixation in the oceans2.
• Zn- Required for enzymes, carbonic anhydrase & alkaline phosphatase3.
• Cd- Found within phytoplankton, high quantities can become toxic 4.

Stable isotope ratios can be used as a tracer for source attribution.

• Anthropogenic-sourced metals have distinct isotopic composition compared to lithogenic dust sourced metals.
• Lithogenic Saharan dust signatures5 : δ⁵⁶Fe = +0.1‰, δ⁶⁶Zn = +0.3‰, and δ¹¹⁴Cd = +0.0‰.

Aerosols are deposited by two mechanisms1:

• Dry Deposition: The settling of …

A Little Blue Hedgehog That Runs A Lot And Has An Attitude: An Analysis Of The Covers Of Archie Comics’ Sonic The Hedgehog, Eleanor Herman

Theses and Dissertations

Through the lens of Robin Williams principles of design as outlined in her book, The Non-Designer’s Design Book, this thesis analyzes selected covers of Archie Comics’ Sonic the Hedgehog comic book series to see how the covers evolved over time, as the nearly 25-year continuous run allows for a unique analysis opportunity. 18 issues were selected and analyzed, with conclusions drawn from the covers’ use of contrast, alignment, repetition, and proximity.

In Situ And Operando Microscopy Studies On Lithium Metal Anodes: A Review, Ian Lindsey, Cameron Mondl, Xiangbo Meng

Mechanical Engineering Faculty Publications and Presentations

Lithium metal is regarded as an ultimate anode for rechargeable batteries, ascribed to its extremely high capacity. Its implementation can remarkably boost the energy density of the resultant lithium metal batteries. However, it is very challenging to commercialize lithium metal anodes, primarily due to the intertwined nature of the formation of a solid electrolyte interphase (SEI) and the growth of lithium dendrites. To understand these issues and therefore develop technical solutions, various instrumental techniques have been employed. This review highlights the most recent advancements of in situ and operando microscopy studies that are critical for investigating and addressing the issues …

Obesity And Long Covid: Intersecting Epidemics?, Michael Gottlieb, Huihui Yu, Ji Chen, Erica S. Spatz, Nicole L. Gentile, Rachel E. Geyer, Michelle Santangelo, Caitlin Malicki, Kristyn Gatling, Kelli N. O'Laughlin, Kari A. Stephens, Joann G. Elmore, Lauren E. Wisk, Michelle L'Hommedieu, Robert M. Rodriguez, Juan Carlos C. Montoy, Ralph C. Wang, Kristin L. Rising, Efrat Kean, Jonathan W. Dyal, Mandy J. Hill, Arjun K. Venkatesh, Robert A. Weinstein, Inspire Group

Jefferson Center for Connected Care Faculty Papers

BACKGROUND: Obesity affects over 10% of the world population and has significant public health implications. With rising recognition of the long-term effects of Long COVID (LC) coupled with new agents to facilitate weight loss, it is critical to understand the influence of obesity on LC. This study assessed the association of obesity with rates of LC and degree of LC-related mental and physical health outcomes among participants up to three years after initial infection.

METHODS: This was a cross-sectional, multisite study of participants with SARS-CoV-2 infection from 12/11/2020–8/29/2022, with data collected through 4/2/2024. Surveys included validated tools for physical and …

Association Between The 2022 Aha/Acc/Hfsa Heart Failure Staging And Cardiovascular And Kidney Outcomes In Patients With Diabetes And Kidney Disease: A Post Hoc Analysis Of The Scored Randomized Controlled Trial, Ayodele Odutayo, Deepak L Bhatt, Vikas S Sridhar, Michael Szarek, Christopher P Cannon, Lawrence A Leiter, Darren K Mcguire, Julia B Lewis, Renato D Lopes, Benjamin M Scirica, Kausik K Ray, Michael J Davies, Phillip Banks, Manon Girard, Subodh Verma, Jacob A Udell, Bertram Pitt, Ph Gabriel Steg, David Z I Cherney

Faculty, Staff and Student Publications

Background: The 2022 American Heart Association/American College of Cardiology/Heart Failure Society of America heart failure (HF) classification incorporates cardiac biomarkers to identify early risk of HF. The HF stages may also guide the prognosis and management of cardiovascular and kidney-related events.

Methods: SCORED (Effect of Sotagliflozin on Cardiovascular and Renal Events in Patients With Type 2 Diabetes and Moderate Renal Impairment Who Are at Cardiovascular Risk) was a randomized trial in diabetes with kidney disease comparing sotagliflozin versus placebo on cardiovascular death, HF hospitalizations, and urgent HF visits. SCORED participants were grouped by HF stage post hoc. Stage A: no …

Single-Photon Emission Computed Tomography Functional Liver Imaging To Facilitate Reirradiation For Liver Malignancies: A Phase I Trial, Enoch Chang, Emma B Holliday, Janio Szklaruk, William D Erwin, Ethan B Ludmir, Sonal S Noticewala, Prajnan Das, Albert C Koong, Bruce D Minsky, Grace L Smith, Gabriel O Sawakuchi, Rachael M Martin-Paulpeter, Joshua S Niedzielski, Luis A Perles, Sam Beddar, Veronica S Valino, Joshua L Nguyen, Kanwal P S Raghav, Milind Javle, Ahmed O Kaseb, Arvind N Dasari, Michael J Overman, Christine M Parseghian, Bruno C Odisio, Armeen Mahvash, Joshua D Kuban, Shu-En Shen, Xin S Wang, Aashish C Gupta, Mais M Al Taie, Kristy K Brock, Ching-Wei D Tzeng, Jean-Nicolas Vauthey, Franklin C L Wong, Eugene J Koay

Faculty, Staff and Student Publications

Background & aims: This phase I trial evaluated the safety of hypofractionated liver reirradiation (reRT) with preferential sparing of functional liver identified by technetium-99m sulfur colloid single-photon emission computed tomography (Tc-99m SC SPECT; hereafter SPECT) for patients previously treated with external beam radiotherapy or transarterial radioembolization.

Methods: A 3+3 design evaluated the safety of two escalating SPECT-based functional liver volumetric dose constraints. Dose-limiting toxicities (DLTs), liver segment volumes, and MRI functional liver imaging score, relative liver enhancement, and elastography were assessed.

Results: Thirteen patients were enrolled from 2018 to 2021. Median reRT gross tumor volume was 61 cm3 (range 1-677 …

The Gene Encoding Ornithine Decarboxylase For Putrescine Biosynthesis Is Essential For The Viability Of Fusobacterium Nucleatum, Shiqi Xu, Bibek G C, Alex Phan, Chenggang Wu

Faculty, Staff and Student Publications

Fusobacterium nucleatum is a Gram-negative anaerobe associated with periodontitis and colorectal cancer. It secretes putrescine, a polyamine that promotes biofilm formation by oral co-colonizers and enhances the proliferation of cancer cells. However, the physiological importance of putrescine for F. nucleatum itself remains unexplored. Here, we show that putrescine biosynthesis, mediated by the ornithine decarboxylase gene oda, is essential for F. nucleatum viability. Deletion of oda was only possible when a functional copy was provided in trans, and CRISPR interference of oda expression resulted in complete growth defects. The essentiality of oda was conserved across multiple subspecies. Supplementation with …

Disease-Specific And Overall Survival For Patients With Lacrimal Gland Adenoid Cystic Carcinoma In Recent Decades, Bita Esmaeli, Zhouxuan Li, Tracy J Lu, Hila Goldberg, Jing Ning, James Matthew Debnam, Steven Jay Frank, Renata Ferrarotto

Faculty, Staff and Student Publications

Purpose: To evaluate the presenting symptoms, and the overall survival (OS) and disease-specific survival (DSS) in patients with lacrimal gland adenoid cystic carcinoma (LGACC).

Methods: This retrospective single-centre cohort study included all consecutive patients with LGACC treated by the primary author from November 1998 through August 2024. Demographic data, presenting symptoms, the histological subtype of LGACC, type of surgical treatment, adjuvant radiotherapy or chemotherapy, T category at presentation and survival data were reviewed. Correlations between histological subtypes, T-category, type of surgery (eye sparing vs orbital exenteration), and DSS and OS were analysed.

Results: 52 patients had a median age of …

Cdk2 Inhibitor Blu-222 Synergizes With Cdk4/6 Inhibitors In Drug Resistant Breast Cancers Through P21/P27 Induction, Linjie Luo, Yan Wang, Tuyen Bui, Xiaoting Jiang, Mei-Kuang Chen, Xiayu Rao, Sepideh Mohammadhosseinpour, Mi Li, Serena Kim, Rachel Y Kim, Saba Kamaliasl, Carmen W Ulizio, Spyros Tsavachidis, Juliana Navarro-Yepes, Nicole M Kettner, Hannah Wingate, Funda Meric-Bernstam, Kelly K Hunt, Jing Wang, Kerrie Faia, Khandan Keyomarsi

Faculty, Staff and Student Publications

Cyclin-dependent kinase 4/6 inhibitors (CDK4/6i) combined with endocrine therapy are the standard first-line treatment for hormone receptor-positive, HER2-negative (HR+/HER2-) metastatic breast cancer, but resistance inevitably develops. In triple-negative breast cancer (TNBC), the efficacy of CDK4/6i remains uncertain. Our study shows that the selective CDK2 inhibitor BLU-222, while effective alone, enhances synergistic activity when combined with CDK4/6i in resistant HR+/HER2- and TNBC models, leading to increased apoptosis and cell cycle arrest. In vivo, combining BLU-222 with palbociclib or ribociclib produced significant antitumor activity across eight resistant models, driving durable tumor regression and prolonged survival. Mechanistically, BLU-222, alone or with palbociclib, upregulated …

Cdk2 Inhibitor Blu-222 Synergizes With Cdk4/6 Inhibitors In Drug Resistant Breast Cancers Through P21/P27 Induction, Linjie Luo, Yan Wang, Tuyen Bui, Xiaoting Jiang, Mei-Kuang Chen, Xiayu Rao, Sepideh Mohammadhosseinpour, Mi Li, Serena Kim, Rachel Y Kim, Saba Kamaliasl, Carmen W Ulizio, Spyros Tsavachidis, Juliana Navarro-Yepes, Nicole M Kettner, Hannah Wingate, Funda Meric-Bernstam, Kelly K Hunt, Jing Wang, Kerrie Faia, Khandan Keyomarsi

Faculty, Staff and Student Publications

Cyclin-dependent kinase 4/6 inhibitors (CDK4/6i) combined with endocrine therapy are the standard first-line treatment for hormone receptor-positive, HER2-negative (HR+/HER2-) metastatic breast cancer, but resistance inevitably develops. In triple-negative breast cancer (TNBC), the efficacy of CDK4/6i remains uncertain. Our study shows that the selective CDK2 inhibitor BLU-222, while effective alone, enhances synergistic activity when combined with CDK4/6i in resistant HR+/HER2- and TNBC models, leading to increased apoptosis and cell cycle arrest. In vivo, combining BLU-222 with palbociclib or ribociclib produced significant antitumor activity across eight resistant models, driving durable tumor regression and prolonged survival. Mechanistically, BLU-222, alone or with palbociclib, upregulated …

Utah Women In The Workplace: Entrepreneurship, Workforce Development, Stem Fields, And Leadership Development, Susan R. Madsen, April Townsend

Marketing and Strategy Faculty Publications

During the fall of 2023, Utah Women & Leadership Project (UWLP) researchers conducted a statewide study to establish a baseline for public perceptions related to the awareness, understanding, and attitudes about challenges that Utah women and girls face. In early 2024, we published 20 short research summaries¹ that shared the results of this comprehensive study (see Nos. 9–28). Next, in the fall of 2024, we administered this survey, with slight changes, around the state of Utah for the second time. Six comprehensive research white papers were then published on the results (see Nos. 11, 12, 13, 14, 16, and …

Exploring Patient, Parent And Clinician Views Of Outcomes For Family-Centered Care In Neonatal Settings: A Qualitative Study, Cousin Study Group, Cansel Kocakabak, Agnes Van Den Hoogen, Jos M. Latour

School of Nursing and Midwifery

Highlights: What are the main findings? Qualitative analysis of family-centered care experiences, drawn from perspectives of parents, a former neonatal patient, and healthcare professionals, identified 42 distinct outcomes which were categorized into five domains. The five domains, with distinct outcomes, highlight the multidimensional family-centered care including: emotional functioning, role functioning, delivery of care, physiological health, and hospital resources. What are the implications of the main findings? These findings show the importance of involving parents, former neonatal patients and healthcare professionals in the development of a robust core outcome set for family-centered care interventions in neonatal settings. The findings provide evidence …

The Spectrum Of Bipolar Disorder In Older Adults, Hui Xin Ng, Martha Sajatovic

Faculty Scholarship

The absolute number and relative proportion of individuals with older-age bipolar disorder (OABD) is expected to rise due to the global aging of the population, necessitating a greater understanding of the unique characteristics of OABD and the trajectory of aging with BD in order to improve the health span of people with BD. This review summarizes current knowledge on OABD, examining its clinical presentation, neurobiology, and treatment, as well as identifying key gaps and future directions for research. OABD is characterized by relatively greater cognitive impairment and somatic burden, despite potentially reduced mood symptom severity compared to younger-age bipolar disorder …

Kenyon Collegian - January 22, 2026

The Kenyon Collegian

No abstract provided.

Subjective Perspectives On Antarctic Wilderness: An Application Of Integral Ecology, Annabelle G. Mayes

Honors College Theses

Parties to the Antarctic Treaty have set Antarctica aside for peace, scientific research, and environmental protection. However, they have yet to take concrete actions to protect Antarctica’s wilderness values as agreed under the 1991 Protocol on Environmental Protection to the Antarctic Treaty. Meanwhile, pristine areas that are free from human interference are declining as human activity escalates. This study examines perceptions of Antarctic wilderness through the lens of integral ecology. Data from 1,640 people and 16 study populations were extracted from four published studies. Content analysis was conducted to determine how subjective perspectives differ across populations and geographic regions. All …

Prehospital Transfusion Strategies In Haemorrhagic Shock, Brian Burns, Brodie Nolan, Ian Ferguson, Michael Peddle, Christopher Partyka, Geoff Healy, Stacy Shackelford, Bryan Cotton

Faculty, Staff and Student Publications

Background: Massive transfusion protocols are established in-hospital practices for managing haemorrhagic shock, yet critical bleeding accounts for up to 40% of trauma deaths, with half occurring before hospital arrival. This has driven interest in prehospital transfusion, a concept originating in military settings where early blood product administration during prolonged evacuations demonstrated significant survival benefits. Despite compelling military evidence showing improved 24-h and 30-day survival with prehospital transfusion, civilian adoption faces substantial challenges. Global interest in this domain is increasing, particularly in regions with trauma populations remote from immediate access to major trauma centres.

Main body: Current prehospital transfusion strategies have …

An Ai Tool Recreates Some Of The Friction Of Academic Search. Is That Actually Helpful?, Jasmine S. Kirby

Libraries Faculty & Staff Publications

Keenious mitigates some of the educational risks of AI search. But its limitations will frustrate some users.

Cross-Ancestry Comparison Of Aptamer And Antibody Protein Measures, Jayna C Nicholas, Daniel H Katz, Usman A Tahir, Catherine L Debban, Francois Aguet, Thomas Blackwell, Russell P Bowler, K Alaine Broadaway, Jingsha Chen, Clary B Clish, Josef Coresh, Elaine Cornell, Daniel E Cruz, Rajat Deo, Margaret F Doyle, Peter Durda, Lynette Ekunwe, James S Floyd, Dipender Gill, Xiuqing Guo, Ron C Hoogeveen, Craig Johnson, Leslie A Lange, Yun Li, Alisa Manning, James B Meigs, Michael Y Mi, Josyf C Mychaleckyj, Nels C Olson, Katherine A Pratte, Brucy M Psaty, Alexander P Reiner, Peifeng Ruan, Magdalena Sevilla-Gonzalez, Amil M Shah, Quan Sun, Russell P Tracy, Jia Wen, Alexis C Wood, James G Wilson, Kristin L Young, Bing Yu, Mary R Rooney, Ani Manichaikul, Ruth Dubin, Karen L Mohlke, Stephen S Rich, Jerome I Rotter, Peter Ganz, Robert E Gerszten, Kent D Taylor, Laura M Raffield

Faculty, Staff and Student Publications

Measures from affinity-proteomics platforms often correlate poorly, challenging interpretation of protein associations with genetic variants and phenotypes. Here, we examine 2157 proteins measured on both SomaScan 7k and Olink Explore 3072 across 1930 participants with genetic similarity to European, African, East Asian, and Admixed American ancestry references. Inter-platform correlation coefficients for these 2157 proteins follow a bimodal distribution (median r = 0.30). We evaluate protein measure associations with genetic variants, and find approximately 25-30% of the signals on each platform are likely driven by protein-altering variants. We highlight 80 proteins that correlate differently across ancestry groups likely in part due …

Ureilite Meteorite Petrofabrics: Evolution And Formation Deformational Conditions, Benjamin Botwe Agyemang

Dissertations and Theses

This thesis examines the origin and development of crystallographic fabrics in ureilites, which are primitive achondritic meteorites interpreted as residues of partial melting from a differentiated parent body. In this study, we evaluate three fabric-forming mechanisms: gravitational cumulate crystallization, shock-induced deformation, and flow alignment associated with melt extraction and convective stirring. A quantitative Electron Backscatter Diffraction (EBSD) analysis was conducted on six ureilitic meteorites to assess olivine Lattice Preferred Orientation (LPO), Shape Preferred Orientation (SPO), the connection between SPO and LPO, and other deformation metrics, including Grain Orientation Spread (GOS), and Crystal Rotation Axis (CRA). Our results suggest that most …

Single-Cell Atlas Of Human Lung Aging Identifies Cell Type Dyssynchrony And Increased Transcriptional Entropy, Ruben De Man, John E Mcdonough, Taylor S Adams, Fadi Nikola, Reina Rangel, Sabina Anderson, Edward P Manning, Juan Cala Garcia, Benjamin Moss, Alan Waich, Fernando Poli, Rafael Cardenas, Cristian Coarfa, Qi Song, Ziv Bar-Joseph, Bart M Vanaudenaerde, Wim A Wuyts, Laura Niklason, Micha Sam B Raredon, Xiting Yan, Ivan O Rosas, Naftali Kaminski

Faculty, Staff and Students Publications

Age is a major risk factor for lung disease. We characterized the changing cellular, transcriptional, and genomic landscape of human lung aging using single-cell RNA sequencing. We find that lung aging is cell-type dyssynchronous, with alveolar epithelial and endothelial cells exhibiting the greatest transcriptional changes. Among alveolar epithelial cells, aging is associated with a decreased relative proportion of surfactant-expressing SPChigh AT2 cells. Among alveolar capillary cells, we observed loss of differentiation and capillary function. Analysis of somatic mutations called from single-cell data revealed an increase with aging, with alveolar epithelial and endothelial cell types exhibiting greater mutation burdens. Transcriptional entropy …