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Articles 40921 - 40950 of 1326674
Full-Text Articles in Entire DC Network
Inorganic Nanoparticle And Nanoplastic Transformations And The Impact On Biouptake By Freshwater Algal Cells Using Single Cell And Single Particle Inductively Coupled Plasma Mass Spectrometry, Elizabeth Carey Bair
Inorganic Nanoparticle And Nanoplastic Transformations And The Impact On Biouptake By Freshwater Algal Cells Using Single Cell And Single Particle Inductively Coupled Plasma Mass Spectrometry, Elizabeth Carey Bair
Theses and Dissertations
The ubiquitous presence of nanoparticles (NPs) and nanoplastics (NPPs) and their effects on environmental and human health is a significant concern. They are used in every aspect of our lives, and while they offer many benefits, there are concerns about their impacts on ecosystems and human health. The primary aim of this dissertation is to quantify NP and NPP transformations in the presence of algae and determine how those transformations influence bioavailability. The methodology involves two complementary analytical techniques: single particle (SP) and single cell (SC) inductively coupled plasma mass spectrometry (ICP-MS). These techniques are tested and applied to two …
Birth Of Inspiration: York County, South Carolina, The Setting That Inspired The Clansman, Zachary Alan Lemhouse
Birth Of Inspiration: York County, South Carolina, The Setting That Inspired The Clansman, Zachary Alan Lemhouse
Theses and Dissertations
In 1905, Thomas Dixon, Jr., published his controversial novel The Clansman. Monopolizing on racist sentiments held by white southerners, Dixon’s narrative contributed significantly to the perception of the Lost Cause myth and defense of white supremacy in America. The novel, and its subsequent adaptations as a play and the film Birth of a Nation, was inspired by actual events that occurred in Dixon’s ancestral home of York County, South Carolina. Located in the northernmost region of South Carolina’s Piedmont, York County played host to a struggle for civil rights during the Reconstruction Era that garnered national and international …
Aesthetic Judgment: Considering A Contemporary Standard Of Taste, Lydia Margaret Lee
Aesthetic Judgment: Considering A Contemporary Standard Of Taste, Lydia Margaret Lee
Theses and Dissertations
Originally published in 1757 as part of his Four Dissertations essay collection, David Hume’s essay, “Of the Standard of Taste,” occupies a prominent place in Enlightenment aesthetic studies. Due to an oversaturation of print materials being produced, eighteenth century society was concerned with evaluating and ranking the massive quantities of art being produced. Philosophers such as David Hume attempted to define criteria for determining a standard of taste, aiming to hierarchically rank works of art based on aesthetic experience. For centuries, critics such as Timothy Costelloe, Noel Carroll, David Marshall, Jonathan Friday, James Shelley, and many others have engaged in …
Strain And Redox Engineering For Enhanced Thermoelectric Performance In Transition Metal Oxide Thin Films, Mohammad Jamal El Loubani
Strain And Redox Engineering For Enhanced Thermoelectric Performance In Transition Metal Oxide Thin Films, Mohammad Jamal El Loubani
Theses and Dissertations
The discovery of highly efficient and cost-effective thermoelectric (TE) materials is essential to envision high-performance TE power generators that enable the direct conversion of waste heat into electricity. Transition metal oxides (TMOs) are considered promising candidates for high-temperature TE applications due to their excellent thermal stability and low cost. However, their maximum power output (power factor, PF = σ•S2) is constrained by the intrinsic trade-off between thermopower (S) and electrical conductivity (σ), both of which are inversely related to carrier concentration. This trade-off poses a significant barrier to improving the performance of TE devices. Among various strategies to overcome this …
Ideologies Of Language, Race, And Time In U.S. Political Discourses, Paige Pinkston
Ideologies Of Language, Race, And Time In U.S. Political Discourses, Paige Pinkston
Theses and Dissertations
This dissertation follows how public discourses that emerged during the 2020- 2022 “Critical Race Theory Panic” played out in different contexts and at different scales in the United States, from conservative national media to South Carolina legislative action. While political discourse has become increasingly nationalized (Berry & Sobieraj, 2014), new legislation has largely taken place at the state level (Johnson, 2022). Drawing on over two years of fieldwork and methods of discourse analysis, I thus examine how nationally circulating discourses about race and racism were taken up by South Carolinians to create partisan alignments and distinctions as well as to …
Help-Seeking, Motivation, And Partner Interactions In Couples Affected By Tramatic Brain Injury, Violet Hodge
Help-Seeking, Motivation, And Partner Interactions In Couples Affected By Tramatic Brain Injury, Violet Hodge
Theses and Dissertations
Traumatic brain injury (TBI) affects individuals and their romantic relationships, creating barriers to help-seeking and impacting relational health. Despite evidence supporting the benefits of couples counseling and relationship education, stigma and motivational factors influence engagement in these services. This dissertation examines the interdependent processes of help-seeking, motivation, and relationship satisfaction in couples where one partner has experienced a TBI. Using the Actor-Partner Interdependence Model (APIM), two studies explore (1) the role of self-stigma in help-seeking and motivation for completing a relationship education program, and (2) the influence of self-stigma for help-seeking on motivation in couples services, particularly in the context …
Autoregressive Modeling Of Dna Molecule Shapes Accompanied By An Empirical Assessment Of The Ljung-Box Test, David William Custer
Autoregressive Modeling Of Dna Molecule Shapes Accompanied By An Empirical Assessment Of The Ljung-Box Test, David William Custer
Theses and Dissertations
The assumption of independence rarely holds in real-world data. Correlated observations are ubiquitous, especially in sequential contexts where time series models are essential for capturing temporal dependence. This study analyzed six groups of damaged and undamaged DNA sequences, where an "F" in the middle of a sequence indicates damage. One biological aim is to understand how DNA regenerates with the assistance of proteins that recognize damaged regions. Motivated by empirical support for AR(2) modeling, we fit autoregressive models to the first three principal component scores of each DNA group, capturing the dominant structure in the data. We conducted model diagnostics, …
Between Tajweed And Lenition: A Sociophonetic Investigation Of Pharyngealized Consonant Variation In Ammani Jordanian Arabic, Amenah Jehad Salman
Between Tajweed And Lenition: A Sociophonetic Investigation Of Pharyngealized Consonant Variation In Ammani Jordanian Arabic, Amenah Jehad Salman
Theses and Dissertations
Thіs dіssеrtаtіоn іnvеstіgаtеs the sосіоphоnеtіс vаrіаtіоn іn thе rеаlіzаtіоn оf phаryngeаlіzеd соnsоnаnts іn Аmmаnі Jоrdаnіаn Аrаbіс (АJА), іntrоduсіng Tаjwееd—thе study оf Qurаnіс phоnоlоgy—аs а sосіаlly sаlіеnt vаrіаblе іnfluеnсіng еvеrydаy spееch. А lеnіtеd-phаryngeаlіzеd (LP) vаrіаnt surfасеs аs аn аcоustіcаlly аnd pеrcеptuаlly іntеrmеdіаtе rеаlіzаtіоn, pоsіtіоnеd bеtwееn fully phаryngeаlіzеd (/sˤ, tˤ, dˤ, ðˤ/) аnd plаіn (/s, t, d, ð/) соnsоnаnts. Whіlе prеvіоus studіеs hаvе ехаmіnеd thе іmpасt оf mасrо-sосіаl fасtоrs suсh аs gеndеr, аgе, sосіоеcоnоmіс stаtus, аnd оrіgіnаl rеgіоnаl dіаlеct (ОRD) (Аl-Маsrі & Jоngmаn, 2004; Аlzоubі, 2017; Аbudаlbuh, 2010), nо rеsеаrch hаs examined thе rоlе оf Tаjwееd іn shаpіng sосіоphоnеtіс оutcоmеs.
Dаtа was …
Queer Inclusion In Libba Bray's Beauty Queens: The Case For Lgbtq Ya Literature, Kayla Michelle Crumbley
Queer Inclusion In Libba Bray's Beauty Queens: The Case For Lgbtq Ya Literature, Kayla Michelle Crumbley
Theses and Dissertations
This thesis is a time sensitive case study on the nature of Libba Bray's novel Beauty Queensand it's placement as both a metacognitive novel and discourse in the nature of queer acceptance in the publishing and media landscape of the 2010s and 2020s. This includes focusing on contextualizing the book in the changing landscape of queer acceptance and visibility in the media over the last 20 years through the lens of the inclusion in YA and how that manifest in the 2010 and 2020s book bans on LGBTQ YA fiction. The novel highlights the wider need for LGBTQ YA …
Emotional Landscapes: St. Teresa Of Avila And The Spiritual Journey, Jillian Louise Breeden
Emotional Landscapes: St. Teresa Of Avila And The Spiritual Journey, Jillian Louise Breeden
Theses and Dissertations
This paper examines the writings of St. Teresa of Avila (1515-1582) through the methodological lens of the history of emotions, arguing that her mystical accounts represent a crucial yet understudied contribution to our understanding of early modern emotional frameworks. Through close textual analysis of Teresa's major works, this study demonstrates how her detailed articulations of spiritual ecstasy, divine love, and religious melancholy offer unique insights into both the religious and secular emotional landscapes of Counter-Reformation Spain. Teresa's innovative emotional vocabulary, which combined traditional Catholic devotional language with embodied metaphors and spatial conceptualizations, allowed her to navigate the complex ecclesiastical politics …
The Penn Center’S Impact On Rural Health Through Midwife Programming In 20Th Century South Carolina, Kathryn Addison Greenberg
The Penn Center’S Impact On Rural Health Through Midwife Programming In 20Th Century South Carolina, Kathryn Addison Greenberg
Theses and Dissertations
The Penn School of the Sea Islands provided an education to thousands of rural, Black South Carolinians over the twentieth century. Northern missionaries including the founders of the Penn School, Laura M. Towne and Ellen Murray, taught and provided healthcare to the residents of the Port Royal Experiment through the Civil War and into Reconstruction. With the arrival of Rossa Cooley and Grace House, the Penn School put emphasis on auxiliary programing including midwifery training institutes held in conjunction with Dr. Hilla Sheriff and the South Carolina Department of Public Health’s Division for Maternal and Child Health. With the goal …
Characterizing The Relationships Between Cranial Geometry, Brain Morphometry, And Brain Mechanical Properties Using Mr Imaging And Elastography, Jessica Restivo
Characterizing The Relationships Between Cranial Geometry, Brain Morphometry, And Brain Mechanical Properties Using Mr Imaging And Elastography, Jessica Restivo
Theses and Dissertations
Traumatic brain injury (TBI) is a global health concern, with over 69 million cases annually. TBI risk depends on subject-specific neuroanatomical variation, yet research often only considers generic finite element (FE) models, based on the geometry and material composition of a 50th percentile male head. Although highly accurate, subject-specific models require expensive magnetic resonance (MR) imaging, elastography, and time-consuming computational resources. There is a need to create subject-specific TBI models that account for subject-specific variations, without neuroimaging. This study aims to create mathematical models to predict brain morphometry (dimensions, volume) [Objective 1] and mechanical properties (shear stiffness, damping ratio, octahedral …
Elevating Next Generation Wireless Devices Towards Contactless Sensing For Healthcare Applications, Aakriti Adhikari
Elevating Next Generation Wireless Devices Towards Contactless Sensing For Healthcare Applications, Aakriti Adhikari
Theses and Dissertations
There is an increasing interest in technologies that can understand and perceive at-home human activities to provide personalized healthcare monitoring, aimed at early detection of disease markers and assisting physicians in making clinical decisions. Existing approaches, such as wearables, require users to wear sensors that can be cumbersome and cause discomfort. Vision based solutions, such as optical cameras, IRs, LiDARs, etc., can be used to design contactless at-home monitoring systems. However, these systems are limited by poor lighting and occlusion, and they are privacy-invasive. Fortunately, high-frequency millimeter-wave wireless devices provide an effective alternative to the existing systems to enable fine-grained …
The Role Of Symmetries In Atomic, Electromagnetic, And Particle Physics, Joshua Martin O'Connor
The Role Of Symmetries In Atomic, Electromagnetic, And Particle Physics, Joshua Martin O'Connor
Theses and Dissertations
Symmetries in atomic, electromagnetic, and weak interaction physics are explored to understand symmetry-breaking extensions of the Standard Model of particle physics. Lorentz-violating field theories are extremely interesting theoretically, since they possess many new features that are absent in Lorentz-invariant models. We outline the formalism and experimental status of the Lorentz- and CPT-violating Standard Model Extension, in both the classical and quantum regimes. Processes such as vacuum Cerenkov radiation, which are kinematically forbidden when Lorentz symmetry is exact may become allowed when this symmetry is weakly broken. Particle decays, such as pion and kaon decays, although allowed in Lorentz-invariant theories, are …
Students’ Experiences Using Peer Review To Affect Writing Self-Efficacy And Writing Performance In An Undergraduate Mass Communications Writing Course, Nina Morrison Brook
Students’ Experiences Using Peer Review To Affect Writing Self-Efficacy And Writing Performance In An Undergraduate Mass Communications Writing Course, Nina Morrison Brook
Theses and Dissertations
The purpose of this action research was to examine the impact of peer review on writing self-efficacy and writing performance in an undergraduate online writing for mass communications course. The study focused on two research questions: (1) How and to what extent does structured peer review affect undergraduate students’ writing self-efficacy in an online writing for mass communications course in a large public Southeastern university? and (2) How and to what extent does structured peer review affect undergraduate students’ writing performance in an online writing for mass communications course in a large public Southeastern university? This research used peer review …
Play, Guide, Aspire: How Game Type And Trainer Involvement Shape Orientation Training Outcomes, Damla Sonmez
Play, Guide, Aspire: How Game Type And Trainer Involvement Shape Orientation Training Outcomes, Damla Sonmez
Theses and Dissertations
The use of gamification in orientation training has gained attention in organizational settings, yet questions remain regarding how different gamified training designs influence new employees’ emotional commitment, willingness to participate in future training, and long-term aspirations. Drawing on Stimulus–Organism–Response (SO-R) theory, Achievement Goal Theory, and Ecological Systems Theory, this dissertation investigates how game type (individual vs. team) and trainer involvement (active vs. passive) shape these outcomes.
Two experimental studies using vignette-based scenarios were conducted. Study 1 tested the direct effects of game type on emotional commitment and willingness to participate. Study 2 employed a 2 (game type) × 2 (trainer …
Function Of Batik For Cross-Gender Instructor At Kinanthi Sekar Dance Center, Yogyakarta, Langen Bronto Sutrisno, Shafinar Ismail, Agus Sudarmawan, Fira Sartika Putri
Function Of Batik For Cross-Gender Instructor At Kinanthi Sekar Dance Center, Yogyakarta, Langen Bronto Sutrisno, Shafinar Ismail, Agus Sudarmawan, Fira Sartika Putri
Bahasa dan Seni: Jurnal Bahasa, Sastra, Seni, dan Pengajarannya
The use of batik in dance teaching at the Kinanthi Sekar Dance Center in Yogyakarta by cross-gender instructors reflects unique and meaningful characteristics. Cross-gender expression in this context is not about failing to conform to traditional gender roles, but rather represents openness, flexibility, and a form of modern cultural embodiment. This study aims to explore and describe the functions of batik for cross-gender instructors at the Kinanthi Sekar Dance Center. Using a qualitative method within a cultural studies perspective, the research applies a focused single-case study approach. The results reveal that batik fulfills various roles, particularly in terms of self-expression, …
Adult English As A Second Or Other Language Peer Oral Interaction In Online Breakout Rooms, Kathleen Moriarty
Adult English As A Second Or Other Language Peer Oral Interaction In Online Breakout Rooms, Kathleen Moriarty
School of Education and Leadership Student Capstone Projects
Peer interaction is essential for adult English as a Second or Other Language (ESOL) students' oral skills development. However, it is more challenging to offer peer interaction in online synchronous classes. To support new teachers who begin online teaching, the author reviewed research on peer learning and online classroom interactional competencies. Based on a foundation of second language acquisition theories and communicative teaching practices, oral skills are developed more with specific types of activities in online breakout rooms: task-based, cooperative, and collaborative activities. These activities are inherently social and motivate learners to engage in negotiation for meaning and a focus …
Checklist For Selling Food At Farmers’ Markets, Mary S. Choate
Checklist For Selling Food At Farmers’ Markets, Mary S. Choate
UNH Cooperative Extension
No abstract provided.
Do-Reflect-Apply In The Showring, Hannah Majewski
Do-Reflect-Apply In The Showring, Hannah Majewski
UNH Cooperative Extension
4-H uses the “Experiential Learning Model” as a way to create high quality youth programs. This model, commonly known as “Do-Reflect-Apply” focuses not only on the particular 4-H experience, but also how youth are interpreting their learning, and if they can apply it to real world scenarios. In the context of the showring, the goal is for youth to dive into their animal projects and learn about industry standards with agriculture, while also developing into capable young adults.
2025 Cornish Fair Exhibit Hall Cards, Extension
2025 Cornish Fair Exhibit Hall Cards, Extension
UNH Cooperative Extension
No abstract provided.
2025 2nd Ticket, Extension
Personal Protective Equipment (Ppe) For Pesticide Use, Rachel Maccini
Personal Protective Equipment (Ppe) For Pesticide Use, Rachel Maccini
UNH Cooperative Extension
No abstract provided.
Conservation Management Effects On Soil And Agronomic Properties In A Furrow-Irrigated Corn System In East-Central Arkansas, Jeferson P. Pimentel, Christopher G. Henry, Kris R. Brye, Benjamin R. Runkle, Amanda J. Ashworth, Edgar Mersiovsky, Kevin C. Thompson
Conservation Management Effects On Soil And Agronomic Properties In A Furrow-Irrigated Corn System In East-Central Arkansas, Jeferson P. Pimentel, Christopher G. Henry, Kris R. Brye, Benjamin R. Runkle, Amanda J. Ashworth, Edgar Mersiovsky, Kevin C. Thompson
Biological and Agricultural Engineering Faculty Publications and Presentations
Cover crops can improve soil physical properties and help to sustain agricultural crop yields. A 6-year study conducted from 2017 to 2022 examined the effects of conservation soil management practices in a furrow-irrigated continuous corn (Zea mays L.) system on a silt-loam soil (Typic Albaqualf) in the Lower Mississippi River Delta region of eastern Arkansas. This study compared the effects of conventional tillage (CT), no-tillage (NT), and no-tillage with cover crops (CC) in selected soil physical and chemical properties (i.e., soil bulk density (D b), porosity (& fnof;t), field capacity (Theta FC), Permanent wilting point (Theta PWP), Plant available water …
The Effect Of Anterior Closing Wedge Slope-Reducing Osteotomy On Coronal Alignment-Effect Of Osteotomy Technique, Starting Point, And Degree Of Correction., James Matthew Helm, Mark Coggins, Brian Crowley, Maile Curbo, Jacob Siahaan, Alexis Aboulafia, Alfred A Mansour
The Effect Of Anterior Closing Wedge Slope-Reducing Osteotomy On Coronal Alignment-Effect Of Osteotomy Technique, Starting Point, And Degree Of Correction., James Matthew Helm, Mark Coggins, Brian Crowley, Maile Curbo, Jacob Siahaan, Alexis Aboulafia, Alfred A Mansour
Faculty, Staff and Student Publications
Background: Techniques for anterior closing wedge slope-reducing osteotomy (ACW-SRO) remain variable regarding management of the tibial tubercle and osteotomy starting point. Moreover, the potential unintended effect on coronal alignment has not yet been determined.
Purpose: To determine the effect of the ACW-SRO technique and starting point on coronal alignment in knees with an elevated posterior tibial slope (PTS).
Study design: Descriptive laboratory study.
Methods: Full-length lower extremity computed tomography scans were retrospectively reviewed in patients presenting to our level 1 trauma center to identify patients with an elevated PTS of ≥12° without secondary trauma to the lower extremity. Materialise software …
A Recipe For Disaster - Sodium Bicarbonate Overdose, Adeola A Kosoko, Amara Ogoke, Kyle Vogt
A Recipe For Disaster - Sodium Bicarbonate Overdose, Adeola A Kosoko, Amara Ogoke, Kyle Vogt
Faculty, Staff and Student Publications
Audience: Emergency medicine residents post-graduate years 1-4.
Introduction: Sodium bicarbonate is a compound found commonplace in many households and in many products. It lends itself to countless everyday uses including cooking and cleaning. Physicians prescribe sodium bicarbonate regularly both on- and off-label regularly for various ailments.1 Due to the ubiquitous way both emergency physicians and the general public use and are exposed to sodium bicarbonate, we wanted to prepare learners to identify and appropriately manage sodium bicarbonate toxicity.Obtaining a thorough history not just of medications prescribed but of significant ingestions or infusions is critical in diagnosing this toxicity. Furthermore, acute …
Mtu-Llm: Llm-Based Multi-Robot Task Allocation And Path Planning For Heterogeneous Robots In Search And Rescue Operations, Kaushik Kannan, Jungyun Bae
Mtu-Llm: Llm-Based Multi-Robot Task Allocation And Path Planning For Heterogeneous Robots In Search And Rescue Operations, Kaushik Kannan, Jungyun Bae
Michigan Tech Publications
Urban Search and Rescue operations after natural disasters involve locating and assisting victims in hazardous environments, which is challenging. Classical Multi-Robot Task Allocation (MRTA) and path planning approaches have been used to deploy heterogeneous robot teams in unsafe areas. However, existing methods often lack focus on workload balance and requirement fulfillment and struggle to generalize across different scenarios. To address these challenges, we propose Multi-robot Task allocation Utilizing LLMs (MTU-LLM), a framework designed to reduce the development time for task allocation and path planning approaches, enabling faster robot deployment. The framework uses an LLM-based “prompt engineering” approach that generates task …
Interconnections Of Insulin/Igf-1 Signaling And Autophagy Abnormalities In Alzheimer’S Disease, Wang Liao, Jinfeng Xuan, Woo-In Ryu, Mariana K Bormann, Yoon Lee, Haley Dion, Elizabeth Evangelista, Ruiyi Li, Lucas Trambaiolli, Jun Liu, Arthur J Siegel, Brent P Forester, Bruce M Cohen, Kai-Christian Sonntag
Interconnections Of Insulin/Igf-1 Signaling And Autophagy Abnormalities In Alzheimer’S Disease, Wang Liao, Jinfeng Xuan, Woo-In Ryu, Mariana K Bormann, Yoon Lee, Haley Dion, Elizabeth Evangelista, Ruiyi Li, Lucas Trambaiolli, Jun Liu, Arthur J Siegel, Brent P Forester, Bruce M Cohen, Kai-Christian Sonntag
Faculty, Staff and Student Publications
Introduction: Impaired insulin (INS) and insulin-like growth factor 1 (IGF-1) signaling are features of both brain aging and late-onset Alzheimer's disease (LOAD). However, their exact underlying mechanisms and cause-and-effect linkages, including the downstream regulation of endocytosis and autophagy, are still not well understood.
Methods: We investigated INS/IGF-1 signaling and its connection with endocytic and autophagic processes in fibroblasts from LOAD patients and healthy young or old control individuals.
Results: Compared to control old-age cells, protein levels in the INS/IGF-1 signaling cascade were elevated in LOAD cells, but activation of AKT was reduced. The activation of the INS/IGF-1/AKT/FOXO1 or mTOR axes …
Arterial Anastomosis To Injured Vessels: Outcomes In Lower Extremity Trauma Reconstruction, Ellen Wang, Jessica R Nye, Imran Rizvi, Kylie R Swiekatowski, Bora Kahramangil, Paul Won, Noa G Kopplin, Stephen F Parlamas, Jeffrey G Trost, Mohin A Bhadkamkar
Arterial Anastomosis To Injured Vessels: Outcomes In Lower Extremity Trauma Reconstruction, Ellen Wang, Jessica R Nye, Imran Rizvi, Kylie R Swiekatowski, Bora Kahramangil, Paul Won, Noa G Kopplin, Stephen F Parlamas, Jeffrey G Trost, Mohin A Bhadkamkar
Faculty, Staff and Student Publications
Background: Although traditional teaching advocates that microsurgical anastomoses should be performed to uninjured vessels outside the zone of injury (ZOI), there is no defined way to assess the extent of the ZOI, and uninjured vessels may not be available. To evaluate the complications ascribed to the ZOI and injured vessels, outcomes between arterial anastomoses fashioned from within the ZOI versus noninjured recipient vessels were compared.
Methods: A retrospective review was conducted of adult patients who required lower extremity free flap (FF) reconstruction from 2016 to 2024. Injury to the recipient artery (transection, avulsion, or thrombosis) was determined by intraoperative assessment …
Automated Mycoplasma Genitalium Molecular Macrolide Resistance Detection And Nucleic Acid Target Semi-Quantitation: Patient Demographic Considerations, Josephine Moore, Trinity Krueger, Amanda Zapp, Stephen C Lavey, Kimber L Munson, Irene A Stafford, Michael E Newcomb, Brian Mustanski, Erik Munson
Automated Mycoplasma Genitalium Molecular Macrolide Resistance Detection And Nucleic Acid Target Semi-Quantitation: Patient Demographic Considerations, Josephine Moore, Trinity Krueger, Amanda Zapp, Stephen C Lavey, Kimber L Munson, Irene A Stafford, Michael E Newcomb, Brian Mustanski, Erik Munson
Faculty, Staff and Student Publications
Recent work has optimized parameters of a real-time reverse transcriptase PCR-based laboratory-developed test on the Panther Fusion system which detects Mycoplasma genitalium-specific macrolide resistance-associated mutations LDT (MRM-LDT) from primary swab and urine specimens. In this study, MRM-LDT was applied to a large multi-demographic study set to further characterize M. genitalium resistance in the United States. A total of 2,145 primary clinical specimens testing positive for M. genitalium 16S rRNA by transcription-mediated amplification (TMA) were initially titered by the same assay using serial 10-fold dilutions to determine relative target nucleic acid burden. Specimens were then processed for MRM-LDT. Findings were stratified …