Digital Commons Network^™

Preparation, Characterization, Antioxidant Activity Of 1-(2-Furoyl) Thiourea Derivatives And Study The Molecular Docking Of Them As Potent Inhibitors Of Urease Enzyme, Oday H. R. Al-Jeilawi, Abdullah Qays Oleiwi

Baghdad Science Journal

In this work, we synthesized thirteen compounds of 1-(2-furoyl)thiourea derivatives 1-13 by conversion of 2-furoyl chloride to 2-furoyl isothiocyanate by reacting it with potassium thiocyanate in dry acetone in a quite short reflux time then, in the same pot, different of (primary and secondary amines) were added individually to achieve thiourea derivatives. The products were characterized spectroscopically using (FT-IR, 1H NMR and 13C NMR) techniques. Some of them were evaluated as antioxidant agents using DPPH radical scavenging method, and all were examined theoretically as enzyme inhibitors against Bacillus pasteurii urease (pdb id: 4ubp) and by studying molecular docking using Autodock …

Synthesis, Characterization And Antioxidant Activity Of New Azo Ligand And Some Metal Complexes Of Tryptamine Derivatives, Myasim Qasim Abdulridha, Abbas Ali Salih Al-Hamdani, Iftikhar Ahmed Hussein

Baghdad Science Journal

New Azo ligand (H2L) was synthesized by reactingthe tryptamine diazonium salt with a compound 4-aminophenol. The characterization was conducted using element microanalysis as well as spectroscopic techniques (UV-Vis, FTIR, 1H & 13C-NMR, and LC-Mass). Complexes for Ni,(II), Pd,(II), Pt,(IV), and Au,(III) were created and identified by using atomic absorption and elemental microanalysis .In addition spectral techniques (infrared, Mass, UV-Vis spectrum were used for magnetic and molar conductance measurements). Then using TGA and DSC curves was to examine the thermal stability of the compounds. Calculating the thermodynamic parameters ΔH, ΔS and ΔG included using the DCS curve. All complexes have a …

The Prevalence Of Electronic Cigarette Use Among College Students Of Taibah University And Symptoms Of Cardiovascular Disease, Talal Alzahrani, Marwan Fahad Alhazmi, Ahmed Naji Alharbi, Feras Talal Alahmadi, Amer Nasser Alhubayshi, Bader Ahmed Alzahrani

Journal of the Saudi Heart Association

Background: Electronic cigarettes (e-cigarettes) have been increasing in popularity among young adults due to the misconception about the safety of e-cigarettes. Our study aims to identify the prevalence rate of e-cigarette use among college students, the reason behind their use, and the relationship between the use of electronic cigarettes and cardiovascular symptoms among college students. Methods: An online questionnaire was sent to students of Taibah University between 2021 and 2022. Data from this survey were analyzed to obtain the prevalence of e-cigarette use among Taibah University students and to compare the demographic and health characteristics differences between e-cigarette users and …

Targeting Energy Intake And Circadian Biology To Engage Mechanisms Of Aging In Older Adults With Obesity: Calorie Restriction And Time-Restricted Eating, Satchidananda Panda, Geraldine Maier, Dennis T Villareal

Faculty, Staff and Students Publications

With the rise in obesity across age groups, it has been a hindrance to engaging in physical activity and mobility in older adults. Daily calorie restriction (CR) up to 25% has been the cornerstone of obesity management even though the safety in older adults remains incompletely understood. Although some adults can follow CR with clinically significant weight loss and improved health metrics, CR faces 2 obstacles-many fail to adopt CR and even among those who can adopt it short term, long-term compliance can be difficult. Furthermore, there is a continuing debate about the net benefits of CR-induced weight loss in …

Workers With Suspected Diagnosis Of Silicosis: A Case Study Of Sarcoidosis Versus Siderosis, Diemen Delgado-García, Patricio Miranda-Astorga, Ashley Delgado-Cano, Juan Gómez-Salgado, Carlos Ruiz-Frutos

School of Medicine Publications

Silicosis is one of the most important occupational respiratory diseases worldwide, hence the importance of making a correct diagnosis. Diagnosis is commonly based on radiological findings according to the ILO International Classification of Radiographs of Pneumoconioses and occupational exposure. High-resolution computed tomography is indicated for differential diagnosis. This article presents two cases with an initial diagnosis of silicosis that ended up being diagnosed as sarcoidosis and siderosis, respectively. The first case was a 42-year-old male who worked as a crushing operator in an underground copper and molybdenum mine for 22 years. He had a history of exposure to silicon dioxide …

Mutations In Lamin And How It Causes Multiple Tissue-Specific Disorders, Bismark Acquah

Theses and Dissertations

Lamins are the major components of the nuclear lamina where they provide a platform for the binding of proteins to the chromatin and confer mechanical stability (Dittmer and Misteli.,2011). Mutations in the human LMNA gene result in at least 15 distinct disorders ranging from muscular dystrophies to neurological disorders to lipodystrophies (Vytopil et al.,2003). Interestingly, some mutant forms of lamin protein aggregate, which may be toxic to the cells. However, it is unknown how specific mutations in lamin give rise to tissue-specific disease. I hypothesize that certain tissues are susceptible to specific lamin mutations due to the inability of tissue-specific …

The City Blues Of An Iridescent Canary: Physiological, Behavioral, And Developmental Impacts Of Lead (Pb) On Songbirds Along An Urban-To-Rural Gradient, Michelle Ross

Master of Science in Integrative Biology Theses

Urbanization is rapidly changing the environment and creating new challenges in the lives of animals across the globe. Anthropogenic contaminants found commonly in old construction materials—including heavy metals like lead— persist within the environment for prolonged periods of time and present a widespread problem for all who live near contaminated areas. Despite the phase-out of lead usage, it continues to threaten the health of all organisms but especially those from urban areas where historical lead use was more common. Currently, the sub-lethal effects of lead on wildlife are not well understood, though lead is known to affect physiology and behavior …

Watering The Soul: Reintroduction Of Water To The Urban Space, Danfei Zhang

Masters Theses

Water and humans have a primordial connection which is more than fundamental survival requirement. Throughout human history, water has been a spiritual archetype by which people perceive and imagine the world. As a reflection of this, water is broadly featured in human material culture and plays a primary role in all religions and cultures.

Well-composed water features are capable for human’s spiritual and physical happiness and wellness. However, water that can benefit human well-being is absent from the vulnerable communities and populations that need it most, which is further exacerbated by the global water crisis.

This thesis proposes the reintroduction …

A Guide To The Brain Initiative Cell Census Network Data Ecosystem, Michael Hawrylycz, Maryann E Martone, Giorgio A Ascoli, Jan G Bjaalie, Hong-Wei Dong, Satrajit S Ghosh, Jesse Gillis, Ronna Hertzano, David R Haynor, Patrick R Hof, Yongsoo Kim, Ed Lein, Yufeng Liu, Jeremy A Miller, Partha P Mitra, Eran Mukamel, Lydia Ng, David Osumi-Sutherland, Hanchuan Peng, Patrick L Ray, Raymond Sanchez, Aviv Regev, Alex Ropelewski, Richard H Scheuermann, Shawn Zheng Kai Tan, Carol L Thompson, Timothy Tickle, Hagen Tilgner, Merina Varghese, Brock Wester, Owen White, Hongkui Zeng, Brian Aevermann, David Allemang, Seth Ament, Thomas L Athey, Cody Baker, Katherine S Baker, Pamela M Baker, Anita Bandrowski, Samik Banerjee, Prajal Bishwakarma, Ambrose Carr, Min Chen, Roni Choudhury, Jonah Cool, Heather Creasy, Florence D'Orazi, Kylee Degatano, Benjamin Dichter, Song-Lin Ding, Tim Dolbeare, Joseph R Ecker, Rongxin Fang, Jean-Christophe Fillion-Robin, Timothy P Fliss, James Gee, Tom Gillespie, Nathan Gouwens, Guo-Qiang Zhang, Yaroslav O Halchenko, Nomi L Harris, Brian R Herb, Houri Hintiryan, Gregory Hood, Sam Horvath, Bingxing Huo, Dorota Jarecka, Shengdian Jiang, Farzaneh Khajouei, Elizabeth A Kiernan, Huseyin Kir, Lauren Kruse, Changkyu Lee, Boudewijn Lelieveldt, Yang Li, Hanqing Liu, Lijuan Liu, Anup Markuhar, James Mathews, Kaylee L Mathews, Chris Mezias, Michael I Miller, Tyler Mollenkopf, Shoaib Mufti, Christopher J Mungall, Joshua Orvis, Maja A Puchades, Lei Qu, Joseph P Receveur, Bing Ren, Nathan Sjoquist, Brian Staats, Daniel Tward, Cindy T J Van Velthoven, Quanxin Wang, Fangming Xie, Hua Xu, Zizhen Yao, Zhixi Yun, Yun Renee Zhang, W Jim Zheng, Brian Zingg

Faculty, Staff and Student Publications

Characterizing cellular diversity at different levels of biological organization and across data modalities is a prerequisite to understanding the function of cell types in the brain. Classification of neurons is also essential to manipulate cell types in controlled ways and to understand their variation and vulnerability in brain disorders. The BRAIN Initiative Cell Census Network (BICCN) is an integrated network of data-generating centers, data archives, and data standards developers, with the goal of systematic multimodal brain cell type profiling and characterization. Emphasis of the BICCN is on the whole mouse brain with demonstration of prototype feasibility for human and nonhuman …

Mesenchymal And Stemness Transdifferentiation Via In-Vitro Infection Of T24 Cell Line With Klebsiella Pneumoniae, Romaila Abd-El-Raouf, Salama A. Ouf, Maha G. Haggag, Khaled F. El-Yasergy, Mahmoud M. Zakaria

Baghdad Science Journal

Klebsiella pneumoniae has been found in the urinary tract of some bladder cancer patients. Bacterial presence within tumor tissue may affect the tumor-microenvironment and consequently influence cancer behavior, development, and treatment response. This study investigated mesenchymal and stemness transdifferentiation of bladder cancer cell line due to environmental stress of K. pneumoniae. Cultures of urothelial bladder cancer cell line (T24) were infected with K. pneumoniae with different multiplicity of infection (MOI) for two and four days. Transdifferentiation-associated features were morphologically assessed. Moreover, transdifferentiation markers were estimated using Q-PCR and immunohistochemistry. Q-PCR data showed an increase in mesenchymal transdifferentiation traits; vimentin expression …

Mn(Ii),Fe(Iii),Co(Ii)And Rh(Iii) Complexes With Azo Ligand: Synthesis, Characterization, Thermal Analysis And Bioactivity, Shaima Mohammed Reda, Abbas Ali Salih Al-Hamdani

Baghdad Science Journal

New series of metal ions complexes have been prepared from the new ligand [4-Amino-N-(5-methyl-isaxazol-3-yl)-benzenesulfonamide] derived from Sulfamethoxazole and 3-aminophenol. Accordingly, mono-nuclear Mn(II), Fe(III), Co (II), and Rh(III) complexes were prepared by the reaction of previous ligand with MnCl2.4H2O, CoCl2.6H2O, FeCl3.6H2O and RhCl3H2O, respectively. The compounds have been characterized by Fourier-transform infrared (FTIR), ultraviolet–visible (UV–vis), mass, 1H-, and 13C-nuclear magnetic resonance (NMR) spectra and thermo gravimetric analysis (TGA& DSC) curve, Bohr magnetic (B.M.), elemental microanalyses, metal ions, chloride containing, and molar conductance.These reviews uncovered octahedral geometries for complexes. The investigation of complexes development by means at molar proportion andoccupation strategy in …

3d Printed Poly Lactic Acid Structures For Cell Growth And Nanoparticle Transport-Simulation And Experimental Validation, Shrikrushna Vinayak Sonawane

Masters Theses

In today's world, 3D printing or additive manufacturing (AM) is being widely used in various fields due to its benefits such as rapid prototyping, reduced material waste and energy usage, lower cost, and more. Even the tissue engineering field is utilizing this technology. This study focused on examining the compatibility of 3D printed Lab-on-a-Chip structures made of Poly Lactic Acid (PLA) for in-vitro applications. In this research, the Lab-on-a-Chip structures were designed and created using 3D printing. To achieve the required bio-functionality scaffold surface was customized by post-printing processes such as mechanical polishing, hydrolysis (Wet Chemical Etching), and ultraviolet light …

Microcalorimeter As A Biologic Activity Monitor For The Study Of Brachiaria Brizantha Seed Germination Process, M. A. Barboza, P. L. O. Volpe, R. Usberti, J. F. G. Faigle, R. H. Aguiar

IGC Proceedings (1977-2023)

Calorimetry helps better understanding of biological processes (Calvet & Prat, 1963). Very sensitive thermal sensors and microcalorimeters allow real time investigation and monitoring heat production of seed germination but few experiments have been performed in this area (Sigstad & Prado, 1999). Moreover, experimental procedures correlating germination phenomena and chemical thermodynamics are exceptional (Barboza, 2002). One can detect calorimetrically the heat flow produced during seed germination and compare the results with data recorded using standard germination methodology (ISTA, 1985). Seed germination and the biomass increase respiration and determination of the energy involved aids understanding of the energetic cycle involved. This work …

Migratory Material: Epigenetics & Weaving At The Us-Mexico Border, Valerie Navarrete

Masters Theses

Discourse often sutures the body shut, disallowing representations of identity to outgrow sociopolitical interests. This issue may originate from borders, but also from the unnamable pathology that generational colonial trauma transmits to the mind, body, and environment. Without a direct form of translatability, this thesis proposes a new materialism that deviates from any object-oriented ontology. Untethered and intra-active, epigenetics and weaving represent objects that transform typical ways of knowing and seeing. Their sensitivity to the environment, in addition to their mobility across generations of time, broaden the spatiotemporal loci of the body and its embodiment. Proposing new materials that expand …

Synthesis Of An Acinetobacter Baumannii Lipooligosaccharide Subunit, James M. Armstrong Ii

LSU Doctoral Dissertations

Acinetobacter baumannii is a Gram-negative, multi drug-resistant bacterium that was placed on the World Health Organization’s watchlist of drug-resistant bacteria. A. baumannii is an ESKAPE pathogen, one of six increasingly antimicrobial-resistant bacteria that are responsible for nosocomial infections. Alternative therapies, such as vaccines, are required to decrease the world’s reliance on antimicrobial agents. Specifically, glycoconjugate vaccines such as those used against Haemophilus influenzae B, Streptococcus pneumoniae, and Neisseria meningitidis are used globally and are highly effective. These vaccines consist of an antigenic bacterial glycan bound to a carrier protein. The glycan is usually a capsular polysaccharide (CPS), however, the notion …

Hello There: An Integrative Framing Analysis Of The Disney+ Series Obi-Wan Kenobi, Hannah Grace Sawchuck

Masters Theses

The effective communication of emotions within audio-visual content, such as film, was one of the most important aspects that needed to be considered when creating such content. Within the field of communication, there was a limited amount of research that existed on the communication of emotions in film. This was a problem as such content is becoming more prevalent in society. Knowing how those emotions were created and developed would allow communication scholars to better understand how to effectively elicit emotions from their own audio-visual content. By examining a well-known series, Obi-Wan Kenobi, scholars could see how different aural and …

'I Don’T Fit That Stereotype': Participant Photography And The Visual (Re)Positioning Of Black Men, Quaylan Allen

Education Faculty Articles and Research

This paper examines how Black men use a participant photography project as a tool for meaning making during the current political and social context of anti-Black racism, Black activism, and intersectional politics. Using a critical visual methodology in the analysis of the images, this paper will examine how Black men make meaning of current social issues through the types of images they produced, how they make meaning of the types of audiences they are speaking to through the project, and how these meanings are reflected through their repositioning practices in the visual project. Specific attention is paid to how they …

Synthesis And Characterization Of Porphyrin-Encapsulated Nanoparticles: Investigations With Scanning Probe Microscopy, Olajumoke Helen Olubowale

LSU Doctoral Dissertations

Scanning probe microscopy (SPM) encompasses a family of surface measurements that use a sharp probe to “feel” a surface. The tip is scanned point-by-point to build a map of the sample topography, which can be correlated with local measurements of surface properties. This dissertation outlines the history of SPM and describes techniques, operational modes, and applications. A review of force volume mapping (FVM), a 3D characterization mode of SPM used to map material properties detailed. Force volume mapping can be achieved by measuring multiple force measurements point-by-point in a grid pattern. Local properties such as chemical forces, dielectric properties, nanomechanical …

Mechanisms Of Kcnq1-Calmodulin Channel Complex Activation To Dual Open States, Po Wei Kang

McKelvey School of Engineering Graduate Student Theses & Dissertations

Voltage-gated potassium channels (KV) facilitate cellular excitability essential for cardiac contraction and neuronal spiking. In response to changes in the membrane potential, KV channels conduct transmembrane K+ flux to terminate action potentials. Myriad drugs target KV channels to achieve therapeutic goals such as anti-arrhythmic and anti-convulsant effects. Owing to their physiological significance, KV channels are exquisitely regulated by a cohort of auxiliary proteins and signaling molecules. The KCNQ1 or KV7.1 voltage-gated potassium channel stands out as an archetype in this regard. Modulators such as the KCNE subunit family, membrane lipid PIP2, and calmodulin (CaM) enable KCNQ1 to conduct phenotypically distinct …

Design And Synthesis Of Donor-Acceptor-Donor Xanthene-Based Near Infrared I And Shortwave Infrared (Swir) Dyes For Biological Imaging, Chathuranga Rathnamalala

Theses and Dissertations

Small molecule organic dyes with absorption and emission in the near infrared region (NIR) attracted much attention for various applications such as dye sensitized solar cells, fluorescent guided surgery, stimuli responsive bioimaging and photodynamic therapy. Dyes with high absorption and emission in the NIR region are beneficial for stimuli responsive bioimaging due to the deeper penetration of NIR light, less cell damage, high resolution, and low background autofluorescence from biomolecules. Of the many small molecule dyes, xanthene-based dyes exhibit outstanding photophysical properties and good stimuli response for use in bioimaging applications. However, absorption and emission of the xanthene dyes lie …

Developing Genotypic And Phenotypic Systems For Early Analysis Of Drug-Resistant Bacteria, Yesman Akuoko

Theses and Dissertations

Antimicrobial resistance in bacteria is a global health challenge with a projected fallout of 10 million deaths annually and cumulative costs of over 1 trillion dollars by 2050. The currently available tools exploited in the detection of bacteria or their DNA can be expensive, time inefficient, or lack multiplex capabilities among others. The research work highlighted in this dissertation advances techniques employed in the phenotypic or genotypic detection of bacteria and their DNA. In this dissertation, I present polymethyl methacrylate-pressure sensitive adhesive microfluidic platforms developed using a time-efficient, inexpensive fabrication technique. Microfluidic devices were then equipped with functionalized monoliths and …

Non-Destructive Imaging Of Phytosulfokine Trafficking In Plants Using Fiber-Optic Fluorescence Microscopy, Bernard Abakah

Electronic Theses and Dissertations

Plants secrete peptide ligands and use receptor signaling to respond to stress and control development. Understanding these phenomena is key to improving plant health and productivity for food, fiber, and energy applications. Phytosulfokine (PSK), a sulfated peptide hormone, regulates plant cell division, growth, and stress tolerance via specific phytosulfokine receptors (PSKRs). This study uses fiber-optic fluorescence microscopy to elucidate trafficking of PSK in live plants. The microscope features two-color optics and an objective lens connected to a 1-m coherent imaging fiber mounted on either a conventional upright microscope body or 5-axis positioning system (X–Y–Z plus pitch and yaw). PSK and …

Using Dna As Scaffolding For The Study Of Fret Enhancement By Surface Plasmons On Gold Nanogratings, Evan Thomas Engelhaupt

Physics & Astronomy Honors Theses

Our lab seeks to enhance the Förster resonance energy transfer (FRET) efficiency using surface plasmons excited on gold nanogratings. Surface plasmons are longitudinal oscillations of conduction electrons that travel along a metal surface. FRET is the transfer of energy between a donor and an acceptor fluorescent molecule, the efficiency of which is strongly dependent on the distance between the donor and acceptor molecules. Additionally, we suspect the surface plasmon enhancement effectiveness depends on the distance between the donor and acceptor and the grating surface. To precisely position the donor and acceptor molecules relative to each other and the gold nanograting, …

Mapping The Functional Interactions At The Tumor-Immune Checkpoint Interface, Behnaz Bozorgui, Elisabeth K Kong, Augustin Luna, Anil Korkut

Faculty, Staff and Student Publications

The interactions between tumor intrinsic processes and immune checkpoints can mediate immune evasion by cancer cells and responses to immunotherapy. It is, however, challenging to identify functional interactions due to the prohibitively complex molecular landscape of the tumor-immune interfaces. We address this challenge with a statistical analysis framework, immuno-oncology gene interaction maps (ImogiMap). ImogiMap quantifies and statistically validates tumor-immune checkpoint interactions based on their co-associations with immune-associated phenotypes. The outcome is a catalog of tumor-immune checkpoint interaction maps for diverse immune-associated phenotypes. Applications of ImogiMap recapitulate the interaction of SERPINB9 and immune checkpoints with interferon gamma (IFNγ) expression. Our analyses …

Research Progress Of Subcritical Extraction Technology In The Field Of Nutrient Component Extraction From Aquatic Products, Wang Wen-Jie, Chen Xiu-Biao, Chen Yun-Yun, Zhou Xu-Xia, Ding Yu-Ting

Food and Machinery

The fundamental extraction principle, production process, and instrument of the subcritical fluid (including water) technology in terms of the aquatic products materials were introduced in this review, and the academic progress of SFE technology in extracting and separating nutritional components from the aquatic products within the recent 10 years were summarized, in terms of proteins/peptides, lipids and polysaccharides from aquatic products. The advantages of different processing factors for the extraction of specific components were summarized, and the future development direction of SFE equipment and technology in the deep processing of aquatic products was prospected.

Advances In Mass Spectrometry On Membrane Proteins, Hsin-Chieh Yang, Weikai Li, Jie Sun, Michael L Gross

2020-Current year OA Pubs

Understanding the higher-order structure of membrane proteins (MPs), which are vital for numerous biological processes, is crucial for comprehending their function. Although several biophysical approaches have been used to study the structure of MPs, limitations exist owing to the proteins' dynamic nature and heterogeneity. Mass spectrometry (MS) is emerging as a powerful tool for investigating membrane protein structure and dynamics. Studying MPs using MS, however, must meet several challenges including the lack of stability and solubility of MPs, the complexity of the protein-membrane system, and the difficulty of digestion and detection. To meet these challenges, recent advances in MS have …

Examining The Different Soil Horizons Of White Oak Mountain, Tn And Their Effect On The Soil Microbial Community, Ji Won Moon, Timothy D. Trott

Research in Biology

Soil microorganisms participate in the diverse interaction within virtually all ecosystems, consequently affecting the associated human and animal population. Identifying how edaphic variables alter the functional and taxonomic diversity of the soil microbial community requires an examination of total microbial soil diversity and abundance. This research study examined the effect of soil depth and horizon on the soil microbial community composition of White Oak Mountain. The soil microbial community was characterized by 16S/18S/ITS rDNA amplicon sequencing of the DNA extract of six samples from the three major soil types identified: HcE, MoE, and uMvC. OTU clustering analysis and both alpha …

Synthetic Errα/Β/Γ Agonist Induces An Errα-Dependent Acute Aerobic Exercise Response And Enhances Exercise Capacity, Cyrielle Billon, Sadichha Sitaula, Subhashis Banerjee, Ryan Welch, Bahaa Elgendy, Lamees Hegazy, Tae Gyu Oh, Melissa Kazantzis, Arindam Chatterjee, John Chrivia, Matthew E Hayes, Weiyi Xu, Angelica Hamilton, Janice M Huss, Lilei Zhang, John K Walker, Michael Downes, Ronald M Evans, Thomas P Burris

Faculty, Staff and Students Publications

Repetitive physical exercise induces physiological adaptations in skeletal muscle that improves exercise performance and is effective for the prevention and treatment of several diseases. Genetic evidence indicates that the orphan nuclear receptors estrogen receptor-related receptors (ERRs) play an important role in skeletal muscle exercise capacity. Three ERR subtypes exist (ERRα, β, and γ), and although ERRβ/γ agonists have been designed, there have been significant difficulties in designing compounds with ERRα agonist activity. Additionally, there are limited synthetic agonists that can be used to target ERRs in vivo. Here, we report the …

Detection Of A Diverse Endophyte Assemblage Within Fungal Communities Associated With The Arundo Leaf Miner, Lasioptera Donacis (Diptera: Cecidomyiidae), Marie-Claude Bon, John A. Goolsby, Guy Mercadier, Fatiha Guermache, Javid Kashef, Massimo Cristofaro, Ann T. Vacek, Alan Kirk

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The larvae of Lasioptera donacis Coutin feed on fungal communities lining galleries within the mesophyll of leaf sheaths of Arundo donax in an aggregative manner. It has been stated that L. donacis could have established a fundamental symbiotic relationship with one fungus, although the fungal composition of these communities remains unsettled. Using a culture-dependent approach and ITS sequencing, the present work characterizes and compares the fungal communities associated with L. donacis in Eurasia with the endophytes of A. donax in Texas where L. donacis is absent. The 65 cultivable isolates obtained from L. donacis fungal communities were sorted into 15 …

Detection Of A Diverse Endophyte Assemblage Within Fungal Communities Associated With The Arundo Leaf Miner, Lasioptera Donacis (Diptera: Cecidomyiidae), Marie-Claude Bon, John A. Goolsby, Guy Mercadier, Fatiha Guermache, Javid Kashef, Massimo Cristofaro, Ann T. Vacek, Alan Kirk

School of Earth, Environmental, & Marine Sciences Faculty Publications

The larvae of Lasioptera donacis Coutin feed on fungal communities lining galleries within the mesophyll of leaf sheaths of Arundo donax in an aggregative manner. It has been stated that L. donacis could have established a fundamental symbiotic relationship with one fungus, although the fungal composition of these communities remains unsettled. Using a culture-dependent approach and ITS sequencing, the present work characterizes and compares the fungal communities associated with L. donacis in Eurasia with the endophytes of A. donax in Texas where L. donacis is absent. The 65 cultivable isolates obtained from L. donacis fungal communities were sorted into 15 …