Digital Commons Network^™

Sickle Red Blood Cell-Derived Extracellular Vesicles Activate Endothelial Cells And Enhance Sickle Red Cell Adhesion Mediated By Von Willebrand Factor, Ran An, Yuncheng Man, Kevin Cheng, Tianyi Zhang, Fang Wang, Erdem Kucukal, William J. Wulftange, Utku Goreke, Allison Bode, Lalitha V. Nayak, Jane A. Little, Umut A. Gurkan

Faculty Scholarship

Endothelial activation and sickle red blood cell (RBC) adhesion are central to the pathogenesis of sickle cell disease (SCD). Quantitatively, RBC-derived extracellular vesicles (REVs) are more abundant from SS RBCs compared with healthy RBCs (AA RBCs). Sickle RBC-derived REVs (SS REVs) are known to promote endothelial cell (EC) activation through cell signalling and transcriptional regulation at longer terms. However, the SS REV-mediated short-term non-transcriptional response of EC is unclear. Here, we examined the impact of SS REVs on acute microvascular EC activation and RBC adhesion at 2 h. Compared with AA REVs, SS REVs promoted human pulmonary microvascular ECs (HPMEC) …

An Entosis-Like Process Induces Mitotic Disruption In Pals1 Microcephaly Pathogenesis, Noelle A Sterling, Jun Young Park, Raehee Park, Seo-Hee Cho, Seonhee Kim

Center for Translational Medicine Faculty Papers

Entosis is cell cannibalism utilized by tumor cells to engulf live neighboring cells for pro- or anti-tumorigenic purposes. It is unknown whether this extraordinary cellular event can be pathogenic in other diseases such as microcephaly, a condition characterized by a smaller than normal brain at birth. We find that mice mutant for the human microcephaly-causing gene Pals1, which exhibit diminished cortices due to massive cell death, also exhibit nuclei enveloped by plasma membranes inside of dividing cells. These cell-in-cell (CIC) structures represent a dynamic process accompanied by lengthened mitosis and cytokinesis abnormalities. As shown in tumor cells, ROCK inhibition completely …

Regrowing The Heart, One Tree At A Time, Jeffrey D Steimle, James F Martin

Faculty, Staff and Students Publications

While many animals can completely repair injured tissues, the mammalian heart possesses limited regenerative capabilities. Yan and Cigliola et al. show that AAV-mediated, zebrafish-derived tissue regeneration enhancer elements (TREEs) can direct pro-regenerative gene expression in injured cardiac tissue of mice and pigs that turn off following repair.

Fire Impacts And Dynamics Of Seasonally Dry Tropical Forest Of East Java, Indonesia, Sutomo, Eddie J. B. Van Etten

Research outputs 2022 to 2026

Background: Seasonally dry tropical forests (SDTFs) are globally important ecosystems which receive less research attention compared to tropical rainforests but are equally under serious threat. The objectives of this paper are to characterize the vegetation structure, diversity and composition of SDTF of Baluran National Park, East Java, Indonesia, and to assess the impact of burning this SDTF and its post-fire recovery. Methods: In the field, we measured floristic composition and dominance at sites with different fire histories in both SDTF and adjacent savannas of Baluran. Remote sensing image analysis was also employed using the MODIS burn area product and various …

Insulin-Like Growth Factor 1 Reduces Coronary Atherosclerosis In Pigs With Familial Hypercholesterolemia, Sergiy Sukhanov, Yusuke Higashi, Tadashi Yoshida, Svitlana Danchuk, Mitzi Alfortish, Traci Goodchild, Amy Scarborough, Thomas Sharp, James S. Jenkins, Daniel Garcia, Jan Ivey, Darla L. Tharp, Jeffrey Schumacher, Zach Rozenbaum, Jay K. Kolls, Douglas Bowles, David Lefer, Patrice Delafontaine

School of Medicine Faculty Publications

Although murine models of coronary atherosclerotic disease have been used extensively to determine mechanisms, limited new therapeutic options have emerged. Pigs with familial hypercholesterolemia (FH pigs) develop complex coronary atheromas that are almost identical to human lesions. We reported previously that insulin-like growth factor 1 (IGF-1) reduced aortic atherosclerosis and promoted features of stable plaque in a murine model. We administered human recombinant IGF-1 or saline (control) in atherosclerotic FH pigs for 6 months. IGF-1 decreased relative coronary atheroma in vivo (intravascular ultrasound) and reduced lesion cross-sectional area (postmortem histology). IGF-1 increased plaque’s fibrous cap thickness, and reduced necrotic core, …

Cellular Zinc Deficiency Impairs Heme Biosynthesis In Developing Erythroid Progenitors, Juyoung Kim, Jaekwon Lee, Moon-Suhn Ryu

Department of Biochemistry: Faculty Publications

Anemia is the most prevalent nutrition-related disorder worldwide. Zinc is an essential trace element for various biological processes in the body, and zinc deficiency has been associated with anemia in humans. However, the molecular mechanisms by which zinc availability alters red blood cell development remain uncertain. The present study identifies the essentiality of zinc during erythroid development, particularly for normal heme biosynthesis. G1E-ER4 mouse cells were used as an in vitro model of terminal erythroid differentiation, which featured elevated cellular zinc content by development. Restriction of zinc import compromised the rate of heme and -globin production and, thus, the hemoglobinization …

Scavenger Receptor B1 Facilitates The Endocytosis Of Escherichia Coli Via Tlr4 Signaling In Mammary Gland Infection, Qamar Taban, Syed Mudasir Ahmad, Peerzada Tajamul Mumtaz, Basharat Bhat, Ehtishamul Haq, Suhail Magray, Sahar Saleem, Nadeem Shabir, Amatul Muhee, Zahid Amin Kashoo, Mahrukh Hameed Zargar6, Abrar A. Malik, Nazir A. Ganai, Riaz A. Shah

Department of Nutrition and Health Sciences: Faculty Publications

SCARB1 belongs to class B of Scavenger receptors (SRs) that are known to be involved in binding and endocytosis of various pathogens. SRs have emerging role in regulating innate immunity and host–pathogen interactions by acting in co-ordination with Toll-like receptors.Query Little is known about the function of SCARB1 in milk-derived mammary epithelial cells (MECs). This study reports the role of SCARB1 in infection and its potential association in TLR4 signaling on bacterial challenge in Goat mammary epithelial cells (GMECs). The novelty in the establishment of MEC culture lies in the method that aims to enhance the viability of the cells …

Ecological Complexity And Avoiding Pest Resurgence: Intuitions From Mathematical Ecology, Theresa Ong, John H. Vandermeer

Dartmouth Scholarship

Pest resurgence is defined by a predictable increase in pests following declines resulting from the implementation of pest management practices. This phenomenon is notorious; familiar in biblical references to cyclical plagues of locust and discussions of pesticide treadmills, where pests resurge following the evolution of pesticide resistance. Targeted “silver-bullet” approaches, whether chemical, mechanical, biological, or genetic in nature, apply a reductionist lens toward the removal of a specific target pest, often ignoring ecological context, by application of said silver-bullet. In this review article, we cover how past and recent developments in theoretical ecology and complex systems dynamics provide a general …

Frederick Douglass And The Original Originalists, Bradley Rebeiro

BYU Law Review

Constitutional scholars incessantly grapple over the significance of the Constitution’s original meaning. More specifically, they are preoccupied with, on the one hand, what that meaning is (if such meaning exists) and, on the other hand, the exact nature of that meaning’s authority (if any) over the Constitution and its interpreters. But this debate is hardly novel. In fact, one of the most compelling voices in U.S. history was immersed in similar debates and, out of the constitutional sparring of his time, forged an arresting theory of constitutional interpretation. Frederick Douglass, once a fierce opponent of the U.S. Constitution, evolved into …

The Failure Of Market Efficiency, William Magnuson

BYU Law Review

Recent years have witnessed the near total triumph of market efficiency as a regulatory goal. Policymakers regularly proclaim their devotion to ensuring efficient capital markets. Courts use market efficiency as a guiding light for crafting legal doctrine. And scholars have explored in great depth the mechanisms of market efficiency and the role of law in promoting it. There is strong evidence that, at least on some metrics, our capital markets are indeed more efficient than they have ever been. But the pursuit of efficiency has come at a cost. By focusing our attention narrowly on economic efficiency concerns—such as competition, …

Full Issue: Summer 2021

DePaul Magazine

Recognizing opportunities is the first step to achieving great things. This issue of DePaul Magazine profiles two accomplished alumni who saw an opportunity to make a difference and seized it. Business alumnus George Ruff saw firsthand the tragedy of homelessness and decided with his wife, Tanya, to support what is now the George and Tanya Ruff Institute of Global Homelessness. Law school alumna Karyn Bass Ehler, concerned about efforts to reduce the number of COVID-19 cases, moved from corporate law to general counsel of the Illinois Department of Public Healh. This issue also looks at the work of several professors …

Therapeutic Targeting Of Prenatal Pontine Id1 Signaling In Diffuse Midline Glioma., Dana Messinger, Micah K. Harris, Jessica R. Cummings, Chase Thomas, Tao Yang, Stefan R. Sweha, Rinette Woo, Robert Siddaway, Martin Burkert, Stefanie Stallard, Tingting Qin, Brendan Mullan, Ruby Siada, Ramya Ravindran, Michael Niculcea, Abigail R. Dowling, Joshua Bradin, Kevin Ginn, Melissa Gener, Kathleen Dorris, Nicholas A. Vitanza, Susanne V. Schmidt, Jasper Spitzer, Jiang Li, Mariella G. Filbin, Xuhong Cao, Maria G. Castro, Pedro R. Lowenstein, Rajen Mody, Arul Chinnaiyan, Pierre-Yves Desprez, Sean Mcallister, Matthew D. Dun, Cynthia Hawkins, Sebastian M. Waszak, Sriram Venneti, Carl Koschmann, Viveka Nand Yadav

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Diffuse midline gliomas (DMG) are highly invasive brain tumors with rare survival beyond two years past diagnosis and limited understanding of the mechanism behind tumor invasion. Previous reports demonstrate upregulation of the protein ID1 with H3K27M and ACVR1 mutations in DMG, but this has not been confirmed in human tumors or therapeutically targeted.

METHODS: Whole exome, RNA, and ChIP-sequencing was performed on the ID1 locus in DMG tissue. Scratch-assay migration and transwell invasion assays of cultured cells were performed following shRNA-mediated ID1-knockdown. In vitro and in vivo genetic and pharmacologic [cannabidiol (CBD)] inhibition of ID1 on DMG tumor growth …

Rare Eif4a2 Variants Are Associated With A Neurodevelopmental Disorder Characterized By Intellectual Disability, Hypotonia, And Epilepsy, Maimuna S Paul, Anna R Duncan, Casie A Genetti, Hongling Pan, Adam Jackson, Patricia E Grant, Jiahai Shi, Michele Pinelli, Nicola Brunetti-Pierri, Alexandra Garza-Flores, Dave Shahani, Russell P Saneto, Giuseppe Zampino, Chiara Leoni, Emanuele Agolini, Antonio Novelli, Ulrike Blümlein, Tobias B Haack, Wolfram Heinritz, Eva Matzker, Bader Alhaddad, Rami Abou Jamra, Tobias Bartolomaeus, Saber Alhamdan, Raphael Carapito, Bertrand Isidor, Seiamak Bahram, Alyssa Ritter, Kosuke Izumi, Ben Pode Shakked, Ortal Barel, Bruria Ben Zeev, Amber Begtrup, Deanna Alexis Carere, Sureni V Mullegama, Timothy Blake Palculict, Daniel G Calame, Katharina Schwan, Alicia R P Aycinena, Rasa Traberg, Genomics England Research Consortium, Sofia Douzgou, Harrison Pirt, Naila Ismayilova, Siddharth Banka, Hsiao-Tuan Chao, Pankaj B Agrawal

Faculty, Staff and Students Publications

Eukaryotic initiation factor-4A2 (EIF4A2) is an ATP-dependent RNA helicase and a member of the DEAD-box protein family that recognizes the 5' cap structure of mRNAs, allows mRNA to bind to the ribosome, and plays an important role in microRNA-regulated gene repression. Here, we report on 15 individuals from 14 families presenting with global developmental delay, intellectual disability, hypotonia, epilepsy, and structural brain anomalies, all of whom have extremely rare de novo mono-allelic or inherited bi-allelic variants in EIF4A2. Neurodegeneration was predominantly reported in individuals with bi-allelic variants. Molecular modeling predicts these variants would perturb structural interactions in key protein domains. …

Vulnerability And Resilience In A Tourism Destination, Sergio Alvarez, Alan Fyall

Rosen Research Review

In an industry that is critically vulnerable to hazards, some destinations are hit harder than others by the same or similar events. UCF Rosen College of Hospitality Management researchers, Dr. Sergio Alvarez and Dr. Alan Fyall, have developed a conceptual model that sees the destination's vulnerability as the sum of its physical, social-cultural, economic, ecological/environmental, and institutional vulnerabilities, nestled within its level of exposure to different hazards. Their framework offers to better equip destination management organizations (DMOs) by identifying vulnerabilities and facilitating more effective planning and decision making.

Innovate Ag Tech, Not Ag Tort: Why Legislative And Judicial Policies Favoring Tech Innovation And Big Ag's Ip Rights In Gmos Will Benefit Agricultural Production And Food Security, Gage Patton

Journal of Intellectual Property Law

Genetically modified organisms ("GMOs") play a critical role in technological advances in agriculture. GMOs contribute to generating higher crop yields, reduce the need for pesticide application, and even reduce food waste. Virtually all GMO technologies are developed by a small number of very large agricultural corporations. These corporations are responsible for the vast majority of research and development efforts for agricultural technologies. Agricultural research and development yields technological innovation, which produces better methods of food production. Therefore, the research and development efforts that big ag corporations engage in benefit everyone by providing better methods of food production. However, some critics …

Inventing The Right Drug: Artificial Intelligence May Just Be The Cure For An Antiquated Patent System, Matthew Hashemi

Journal of Intellectual Property Law

The transformation of the inventive process through the application of Artificial Intelligence, or “AI”, technology has radically enhanced the way pharmaceutical companies research and develop new drugs. While AI technology has led to improved accessibility to essential drugs and significant reductions in the time and cost required for drug research and development, it has also left the pharmaceutical industry with the puzzling question regarding how to encourage innovation with AI technology if AI machines cannot obtain patent protection under the current United States patent system. Granting patent protection ensures inventors can profit off their inventions, since patented inventions cannot be …

Innocent Until Proven Posted: Regulating Online Mugshot Publication With Intellectual Property Law, Amanda Cheek

Journal of Intellectual Property Law

Whether convicted of a crime or booked into jail for a misunderstanding, many individuals rightfully seek to shed the criminal label from their reputations and start anew. However, photo evidence of the individual’s association with the criminal justice system by way of a published mugshot photograph is often only a click away for someone, such as a potential employer or new neighbor. Mugshots, originally created to aid in the detection of criminals, now serve primarily as a device of public shaming by law enforcement agencies who publish mugshots on their website and groups of private citizens who run social media …

Universalizing Copyright Fair Use: To Copy, Or Not To Copy?, Taysir Awad

Journal of Intellectual Property Law

This Article thoroughly examines the universal shift toward the U.S. fair use doctrine. It begins with an elucidation of the fair use doctrine and its foreign counterparts, briefly explaining the history and function of each doctrine and differentiating them from one another. The Article then delves into the aspirations of foreign countries that have opted into the transplant of the U.S. doctrine or merely contemplated a transplant. These aspirations are narrowed down to three dimensions of development: access to knowledge, responsiveness to innovative technologies, and enhancing creative production. Using the capabilities approach of development – developed by Amartya Sen and …

A Scoping Review Of Campus-Based Animal-Assisted Interactions Programs For College Student Mental Health, Tanya K. Bailey

People and Animals: The International Journal of Research and Practice

Background: People have long found support by interacting with animals, which has developed into a health care modality called animal-assisted interactions (AAI). In the past 10 years, AAI has increased as a way to support college students’ mental health; however, there is no comprehensive evidence on the effectiveness of these programs.
Method: A scoping review was conducted using the JBI and PRISMA-ScR criteria. Empirical articles were identified through Academic Search Premier (EBSCOhost), PsychINFO (Ovid), and Web of Science using three groups of keywords: AAI, college students, and mental health.
Results: Of the 1,195 publications identified, 37 met this study’s eligibility …

Preparing For The Next Disaster: Lessons Learned From The Early Experiences Of Autistic Adults During The Covid-19 Pandemic, Amber Pomponio Davidson, Cecilia Feeley, Beth Pfeiffer, Mark Salzer

Internet Journal of Allied Health Sciences and Practice

Purpose: Disasters, such as the worldwide COVID-19 pandemic, create significant disruptions for individuals and their communities. Understanding these disruptions is a first step towards planning for future events and disaster recovery. This research aims to explore how the initial COVID-19 restrictions, including lockdowns, impacted adults with Autism (ASD). Method: Using semi-structured interviews to understand how individuals with ASD experienced the early months of the COVID-19 pandemic, twelve participants were interviewed: six individuals with ASD (ages 20-38), three parents of individuals with ASD, and three support staff for individuals with ASD. Analysis: Grounded theory methods were used to analyze the impacts …

Physiochemical Characterization Of Coptic Jesus Christ Icon, Church Of Saint Mercurius, Egypt, Neven Kamal, Shaaban Abdelaal, Emil Henin

Journal of the General Union of Arab Archaeologists

التوصيف الفيزيو كيميائي لايقونة السيد المسيح ، كنيسة ابى سيفين ،جمهورية مصر العربية [Ar]قدم هذا البحث التوصيف الفيزوكيميائى لأيقونة قبطية من القرن التاسع عشر من كنيسة القديس مرقوريوس، حيث تمثل مثال فريد من هذة الايقونات التى تعود الى العصر القبطى. ولكى يتحقق الغرض من الدراسة تم استخدام بعض الوسائل الفحص والتحليل لشرح وايضاح كل مظاهر التلف مما يساعد في التوثيق الأثري للأيقونة ، وقد يعطي ذلك تفسير عن ميكانيكة تلف الأيقونات الأثرية . كشف الفحص البصري أن الأيقونة القبطية تتكون من أربع طبقات: دعامة لوح خشبي ، وطبقة تحضير ، وطبقة طلاء ، وبقية طبقة الورنيش ، وقد عانت …

The Geometric Kufic Inscriptions On The Late Mamluk Architecture And Artifacts: A New Perspective Through Some Artifacts, Waleed Ahmed Salah Al-Din

Journal of the General Union of Arab Archaeologists

الخط الكوفي الهندسي على العمارة والتحف الفنية من العصر المملوكي الجركسي. منظور جديد من خلال بعض التحف الفنية. [Ar]

لقد أظهر الفنانون المسلمون براعتهم في إنتاج العديد من التحف الفنية بأشكالها وزخارفها العديدة والمميزة التي لازال الكثير منها بحاجة ماسة للدراسة والفحص. وقد أطنب فنانو العصر المملوكي في زخرفة تحفهم الفنية بالعديد من الزخارف المختلفة ومن أبرز هذه الزخارف هو الخط الكوفي الهندسي الذي لعب دورا حيوياً وبارزا في الفن المملوكي. ظهر الخط الكوفي الهندسي على العديد من المباني الأثرية التي ترجع إلى العصر المملوكي، ومن الملاحظ ندرة وجوده على الفنون والتحف التي ترجع إلى تلك الحقبة من الزمن، لذا …

Rainmaking Through The Rock Art In East And South Africa: A Comparison With Current Traditional Tribes, Zainab Khamis

Journal of the General Union of Arab Archaeologists

رموز صناعة المطر من خلال الفن الصخري في شرق وجنوب أفريقيا: مقارنة بالقبائل البدائية الحالية [AR]

تعد صناعة المطر واحدة من أهم الطقوس الحياتية منذ عصور ما قبل التاريخ لا سيما خلال العصر الحجري الحديث، وذلك بسبب تحول الطقس إلى الجاف في ذلك الوقت، فأصبح للمطر دور مهم في الحياة، ومن ثم يحاول البحث توضيح أهمية طقوس صناعة المطر كمصدر للمياه ودور صانع المطر ومكانته المميزة فى مجتمعات شرق وجنوب إفريقيا خلال عصور ما قبل التاريخ، وذلك من خلال دراسة مناظر صناعة المطر ورموزه فى الفن الصخرى آنذاك. تباينت رموز صناعة المطر فى الفن الصخري ما بين الرموز الحيوانية والهندسية …

Some Remarks On The Execution Place Xbt In Ancient Egypt, Mona Abou El Maati El Nadi

Journal of the General Union of Arab Archaeologists

بعض الملاحظات عن مكان الاعدام (xbt) في مصر القديمة [AR]

فى هذه المقالة ، تحاول الباحثة إلقاء الضوء على أحد أماكن العقاب في مصر القديمة ، ألا وهو مكان الإعدام الذى يسمى Xbt . ومن خلال الدراسة، نلاحظ أنه وعلي الرغم من تعدد أماكن العقاب وتنوعها فى مصر القديمة ، إلا أن التفاصيل قليلة فى وصف هذه الأماكن ، ولم ينتشر تصويرها سواء فى المناظر أو فى كتب العالم الآخر. وقد كان الإعدام بقطع الرأس موجود فى التصاوير منذ العصور المبكرة، وكان عقابا لأعداء الملك كما سجل على بعض الصلايات من العصور المبكرة وبصفة خاصة صلاية الملك نعرمر، ولكن عقوبة …

Progress Towards The Total Synthesis Of Frondosin D Lab Report, Admirabilis Kalolella

CISLA Senior Integrative Projects

No abstract provided.

Rapid Evolution Of The Fine-Scale Recombination Landscape In Wild House Mouse (Mus Musculus) Populations., Lydia K Wooldridge, Beth L Dumont

Faculty Research 2023

Meiotic recombination is an important evolutionary force and an essential meiotic process. In many species, recombination events concentrate into hotspots defined by the site-specific binding of PRMD9. Rapid evolution of Prdm9's zinc finger DNA-binding array leads to remarkably abrupt shifts in the genomic distribution of hotspots between species, but the question of how Prdm9 allelic variation shapes the landscape of recombination between populations remains less well understood. Wild house mice (Mus musculus) harbor exceptional Prdm9 diversity, with >150 alleles identified to date, and pose a particularly powerful system for addressing this open question. We employed a coalescent-based approach to construct …

Final Status Sheet, Colorado General Assembly

Colorado House and Senate Journals

No abstract provided.

Final Status Sheet, Colorado General Assembly

Colorado House and Senate Journals

No abstract provided.

Five Ideas For How Professors Can Deal With Gpt-3 ... For Now, Travis Ryan Pickell, Brian R. Doak

Faculty Publications - George Fox School of Theology

"The most immediate question that needs to be addressed is pedagogical: how can we continue to teach in the GPT Age?...Beyond the questions of pedagogical best practices, GPT-3 raises deeper philosophical and pragmatic questions about the nature and purpose of higher education."

Saint Augustine, Music, And God, Lois Eveleth

Faculty and Staff - Articles & Papers

Concerned that his love of music might only strengthen his sensuality and thus jeopardize his conversion to Christianity, Saint Augustine produced "De Musica". This saving response identified "numeri" (numbers or ratios) existing in music and serving as structures placed in the human soul by God to guide souls through finite time into eternity. Such structures were congruent with time itself and with the "numeri" which guided the cosmos. Added to this project was the task of rejecting the ancient notion of time as cyclical.