Digital Commons Network^™

Probing Amyloid-Beta Protein Structure And Dynamics With A Selective Antibody, Shikha Grover

Dissertations

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder. The AD brain is characterized by significant neuronal loss and accumulation of insoluble fibrillar amyloid-β protein (Aβ) plaques and tau protein neurofibrillary tangles in the brain. However, over the last decade, many studies have shown that the neurodegenerative effect of Aβ may in fact be caused by various soluble oligomeric forms as opposed to the insoluble fibrils. Furthermore, the data suggest that a pre-fibrillar aggregated form, termed protofibrils, mediates direct neurotoxicity, and triggers a robust neuroinflammatory response.

Antibodies targeting the various conformation of Aβ are important therapeutic agents to prevent the progression …

Kentucky Annual Economic Report 2023, Michael Childress, Michael W. Clark, James P. Ziliak, Simon Sheather

Kentucky Annual Economic Report

This report is one of the important ways that the Center for Business and Economic Research fulfills its mission to examine various aspects of Kentucky’s economy as directed by the Kentucky Revised Statutes (KRS 164.738). The analysis and data presented here cover a variety of topics that range from a discussion of Kentucky’s current economic climate to a broad presentation of factors affecting the economy.

Solidarity And Strife After The Atlanta Spa Shootings: A Mixed Methods Study Characterizing Twitter Discussions By Qualitative Analysis And Machine Learning, Shaniece Criss, Thu T. Nguyen, Eli K. Michaels, Gilbert C. Gee, Mathew V. Kiang, Quynh C. Nguyen, Samantha Norton, Eli Titherington, Leah Nguyen, Isha Yardi, Melanie Kim, Nhung Thai, Ariel Shepherd, Chris J. Kennedy

Open Access Fund Publications

Background: On March 16, 2021, a white man shot and killed eight victims, six of whom were Asian women at Atlanta-area spa and massage parlors. The aims of the study were to: (1) qualitatively summarize themes of tweets related to race, ethnicity, and racism immediately following the Atlanta spa shootings, and (2) examine temporal trends in expressions hate speech and solidarity before and after the Atlanta spa shootings using a new methodology for hate speech analysis.

Methods: A random 1% sample of publicly available tweets was collected from January to April 2021. The analytic sample included 708,933 tweets using race-related …

Deep Learning Architectures For Visual Question Answering On Medical Images, Venkat Ramana Kodali Kodali

Theses and Dissertations

The purpose of this research is to apply both computer vision and natural language processing techniques for visual question answering (VQA) on a medical image dataset. Deep learning and machine learning libraries were used in the research. The research includes understanding key achievements in the field of visual question answering, identifying techniques applied in general images and applying them along with new techniques to medical images. There are many more articles explaining the application of visual question answering to general images than on applying VQA specifically to medical images. In this research, I initially developed a model of VQA that …

Fifty Years Of Global Cardiovascular Research In Africa: A Scientometric Analysis, 1971 To 2021, Jean Jacques Noubiap, Dominic Millenaar, Dike Ojji, Q Eileen Wafford, Christian Ukena, Michael Böhm, Karen Sliwa, Mark D Huffman, Felix Mahfoud

2020-Current year OA Pubs

Background To analyze the quantity and impact of cardiovascular research done in Africa or coauthored by researchers based in Africa, their determinants, and the patterns of research collaboration. Methods and Results We retrieved data from Web of Science and additional sources. We analyzed temporal trends from 1971 to 2021 and geographical distribution, research impact using country-level h-index, international research cooperation, and associations of research quantity and quality using linear regression. The annual volume of cardiovascular research from Africa has increased from 4 publications in 1971 to 3867 in 2020 and currently represents ~3% of the global cardiovascular research output. Authors …

The Role Of The Neurodevelopmental Disorder Gene Myt1l In Mammalian Brain Development, Jiayang Chen

Arts & Sciences Graduate Student Theses and Dissertations

Recent human genetic studies have associated mutations in a gene called Myelin Transcription Factor 1 Like (MYT1L) with neurodevelopmental disorders (NDDs). Patients with MYT1L loss of function (LoF) mutations (MYT1L Syndrome patients) demonstrate shared symptoms such as microcephaly, attention deficit and hyperactivity disorder (ADHD), and obesity. Despite prior studies showing MYT1L overexpression facilitates neuronal differentiation in vitro, its functions in vivo, especially in the mammalian brain, and how its mutation leads to human disease pathology remains poorly understood. Here, I established the first mouse model of MYT1L Syndrome mimicking a patient specific LoF mutation. I found mice with Myt1l heterozygous …

Strange Statutory Foundations And Terribly Tricky Treasures In Trust, Thomas Simmons

Faculty Publications

This paper summarizes two distinct topics: statutory foundations and a varied collection of unique and potentially troubling kinds of assets that might be held by fiduciaries such as trustees. Statutory foundations have recently been legislative approved in two states; Wyoming and New Hampshire and they represent new and interesting planning opportunities for estate planners. Tricky assets can range from the difficult-to-inventory (such as comic books and baseball cards) to outright contraband.

Structures Of Human Gastrin-Releasing Peptide Receptors Bound To Antagonist And Agonist For Cancer And Itch Therapy, Shuman Peng, Yuting Zhan, Dongqi Zhang, Lu Ren, Anqi Chen, Zhou-Feng Chen, Haitao Zhang

2020-Current year OA Pubs

Gastrin releasing peptide receptor (GRPR), a member of the bombesin (BBN) G protein-coupled receptors, is aberrantly overexpressed in several malignant tumors, including those of the breast, prostate, pancreas, lung, and central nervous system. Additionally, it also mediates non-histaminergic itch and pathological itch conditions in mice. Thus, GRPR could be an attractive target for cancer and itch therapy. Here, we report the inactive state crystal structure of human GRPR in complex with the non-peptide antagonist PD176252, as well as two active state cryo-electron microscopy (cryo-EM) structures of GRPR bound to the endogenous peptide agonist gastrin-releasing peptide and the synthetic BBN analog …

Territorial Intrusion By Resident Male Wilson's Warblers: Breeding Stages Of The Intruders And Distances They Travel, William M. Gilbert

Western North American Naturalist

It now is generally recognized that genetic monogamy is the exception in birds, and that extrapair paternity in territorial species requires that one or the other sex involved in such an extrapair mating travel off its own territory to obtain an extrapair copulation. However, which sex or sexes travel off their home territories, the distances they might travel to obtain extrapair copulations, and the breeding stages during which they might make such off-territory forays are poorly understood for most species. I explored these issues in Wilson’s Warblers (Cardellina pusilla) in central, inner-coastal California. Male Wilson’s Warblers frequently intruded …

Independent - Feb. 7, 2023, Monty Stites

The Independent (1988-present)

No abstract provided.

Examining The Intentions To Seek Postgraduate Education (Professional Degree) Amongst University Students In China : The Moderating Role Of Chinese Social Network, Ao Liang

Lingnan Theses (Doctoral degree)

Background: In 2020, the Degree Committee of the State Council collaborated with the Ministry of Education in advocating the policy of ‘Postgraduate education of professional degree development plan (2020–2025), to continuously increase postgraduate education (professional degree) enrolment. Policy implementations by the Chinese government on the development of postgraduate educations how that many efforts and inputs have been made previously on the professional degree of the Chinese government (e.g., reducing the corresponding quota number of 5%–10% of academic degree and providing near 1.2 trillion RMB for the development of professional degrees).However, the results of expansion on professional postgraduate degrees have not …

The Link February 7, 2023, Access To Justice Institute

The Link Newsletter

No abstract provided.

Production Of A Monoclonal Antibody For The Detection Of Forchlorfenuron: Application In An Indirect Enzyme-Linked Immunosorbent Assay And Immunochromatographic Strip, Xingmei Lei, A M Abd El-Aty, Lingyuan Xu, Jing Zhao, Jia Li, Song Gao, Yun Zhao, Yongxin She, Fen Jin, Jing Wang, Lufei Zheng, Maojun Jin, Bruce D Hammock

Faculty, Staff and Student Publications

In this study, a monoclonal antibody (mAb) specific to forchlorfenuron (CPPU) with high sensitivity and specificity was produced and designated (9G9). To detect CPPU in cucumber samples, an indirect enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold nanobead immunochromatographic test strip (CGN-ICTS) were established using 9G9. The half-maximal inhibitory concentration (IC50) and the LOD for the developed ic-ELISA were determined to be 0.19 ng/mL and 0.04 ng/mL in the sample dilution buffer, respectively. The results indicate that the sensitivity of the antibodies prepared in this study (9G9 mAb) was higher than those reported in the previous literature. On the other …

Emerging Technologies For Indigenous Knowledge Management By Public Libraries Of Akwa Ibom State, Daniel Aloysius, Mercy Daniel Ebong Ph.D

Library Philosophy and Practice (e-journal)

This paper examines emerging technologies for indigenous knowledge management by public libraries of Akwa Ibom State. It conceptualise indigenous knowledge, indigenous knowledge management and emerging technologies. The different Indigenous knowledge of Akwa Ibom State were explored. The emerging technologies for management of indigenous knowledge were evaluated. The paper concluded that indigenous knowledge is the identity and prestige of the local people as their cultural heritage over centuries are embedded in it. Hence, it should be adequately managed by public libraries for posterity of the local people and indeed the world over. The paper recommends among others that government should adequately …

Chimeric Antigen Receptor T-Cell Therapy And Hematopoiesis, Bryanna Reinhardt, Patrick Lee, Joshua P. Sasine

School of Medicine Faculty Publications

Chimeric Antigen Receptor (CAR) T-cell therapy is a promising treatment option for patients suffering from B-cell- and plasma cell-derived hematologic malignancies and is being adapted for the treatment of solid cancers. However, CAR T is associated with frequently severe toxicities such as cytokine release syndrome (CRS), immune effector cell-associated neurotoxicity syndrome (ICANS), macrophage activation syndrome (MAS), and prolonged cytopenias—a reduction in the number of mature blood cells of one or more lineage. Although we understand some drivers of these toxicities, their mechanisms remain under investigation. Since the CAR T regimen is a complex, multi-step process with frequent adverse events, ways …

A Proposed Meta-Reality Immersive Development Pipeline: Generative Ai Models And Extended Reality (Xr) Content For The Metaverse, Jay Ratican, James Hutson, Andrew Wright

Faculty Scholarship

The realization of an interoperable and scalable virtual platform, currently known as the “metaverse,” is inevitable, but many technological challenges need to be overcome first. With the metaverse still in a nascent phase, research currently indicates that building a new 3D social environment capable of interoperable avatars and digital transactions will represent most of the initial investment in time and capital. The return on investment, however, is worth the financial risk for firms like Meta, Google, and Apple. While the current virtual space of the metaverse is worth $6.30 billion, that is expected to grow to $84.09 billion by the …

2023 Sdsu Data Science Symposium Presentation Abstracts, Department Of Mathematics & Statistics

SDSU Data Science Symposium

This document contains abstracts for presentations and posters 2023 SDSU Data Science Symposium.

Lanthorn, Vol. 57, No. 21, February 6, 2023, Grand Valley State University

Volume 57, August 1, 2022 - April 10, 2023

Lanthorn is Grand Valley State's student newspaper, published from 1968 to the present.

Ecophysiological Implications Of Low Lignin In Eastern Leatherwood (Dirca Palustris L.), Anna Cole, Kayla Marie Rigdon, D. Gordon Brown, Katharine Lora Cary

Biology Faculty Publications

Dirca palustris L. (eastern leatherwood) is an understory shrub found throughout much of eastern North America. Dirca palustris wood has a low concentration of lignin, which is a molecule that grants structural rigidity to cell walls. The branches of D. palustris are thus remarkably flexible, but their low lignin content could cause greater vulnerability to water stress. We examined the conductivity, vessel anatomy, and field water potential of D. palustris and conducted ecological surveys. The data showed that D. palustris was not experiencing substantial drought-induced dysfunction in the field, even during an exceptionally hot and dry summer. Its water potentials …

Rotunda - Vol 102, No 5 - Feb 6, 2023, Longwood University

Rotunda

No abstract provided.

Broad Efficacy Of A Computationally Designed Ace2 Decoy Against Sars-Cov-2 Omicron Variants And Related Viruses In Vitro And In Vivo, Brandon Havranek, Graeme W. Lindsey, Yusuke Higuchi, Yumi Itoh, Tatsuya Suzuki, Toru Okamoto, Atsushi Hoshino, Erik Procko, Shahidul M. Islam

Alpha Omega Alpha Research Symposium Posters

Background: The SARS-CoV-2 omicron variant (B.1.1.529) and its sublineages are currently the dominant variants in the United States accounting for 100% of COVID-19 cases. Problem: The S protein receptor-binding domain (RBD), located in the S1 subunit of the S protein, binds the human angiotensin-converting enzyme 2 (hACE2) leading to S1 shedding and proteolytic processing of S2 that is important for membrane fusion and release of viral RNA. Various neutralizing therapeutics including protein minibinders, peptides, monoclonal antibodies, and nanobodies have been developed to block the critical interaction between the RBD and hACE2. However, these therapeutics are often developed against the S …

Hawks' Herald -- Feb. 6, 2023, Roger Williams University

Hawk's Herald

No abstract provided.

Newsroom, Georgia Southern University

Newsroom (2006-2023)

Georgia Southern Investing $1.2 Million to Upgrade Campus Security Cameras

Eagle Nation Awards Three Military Spouses Scholarships

The P97-Ubxd8 Complex Regulates Er-Mitochondria Contact Sites By Altering Membrane Lipid Saturation And Composition, Rakesh Ganji, Joao A Paulo, Yuecheng Xi, Ian Kline, Jiang Zhu, Christoph S Clemen, Conrad C Weihl, John G Purdy, Steve P Gygi, Malavika Raman

2020-Current year OA Pubs

The intimate association between the endoplasmic reticulum (ER) and mitochondrial membranes at ER-Mitochondria contact sites (ERMCS) is a platform for critical cellular processes, particularly lipid synthesis. How contacts are remodeled and the impact of altered contacts on lipid metabolism remains poorly understood. We show that the p97 AAA-ATPase and its adaptor ubiquitin-X domain adaptor 8 (UBXD8) regulate ERMCS. The p97-UBXD8 complex localizes to contacts and its loss increases contacts in a manner that is dependent on p97 catalytic activity. Quantitative proteomics and lipidomics of ERMCS demonstrates alterations in proteins regulating lipid metabolism and a significant change in membrane lipid saturation …

Widespread Contribution Of Transposable Elements To The Rewiring Of Mammalian 3d Genomes, Mayank N K Choudhary, Kara Quaid, Xiaoyun Xing, Heather Schmidt, Ting Wang

2020-Current year OA Pubs

Transposable elements (TEs) are major contributors of genetic material in mammalian genomes. These often include binding sites for architectural proteins, including the multifarious master protein, CTCF, which shapes the 3D genome by creating loops, domains, compartment borders, and RNA-DNA interactions. These play a role in the compact packaging of DNA and have the potential to facilitate regulatory function. In this study, we explore the widespread contribution of TEs to mammalian 3D genomes by quantifying the extent to which they give rise to loops and domain border differences across various cell types and species using several 3D genome mapping technologies. We …

Ion Channel Regulation Of Gut Immunity, Jing Feng, Zili Xie, Hongzhen Hu

2020-Current year OA Pubs

Mounting evidence indicates that gastrointestinal (GI) homeostasis hinges on communications among many cellular networks including the intestinal epithelium, the immune system, and both intrinsic and extrinsic nerves innervating the gut. The GI tract, especially the colon, is the home base for gut microbiome which dynamically regulates immune function. The gut's immune system also provides an effective defense against harmful pathogens entering the GI tract while maintaining immune homeostasis to avoid exaggerated immune reaction to innocuous food and commensal antigens which are important causes of inflammatory disorders such as coeliac disease and inflammatory bowel diseases (IBD). Various ion channels have been …

Taking Initiative: Utilizing Louisiana Law To Protect Honey Bees And Fill Regulatory Gaps In Federal Pesticide Laws, Bailey Anderson

LSU Journal of Energy Law and Resources

No abstract provided.

Fish Better Have My Money: Efforts To Combat Illegal, Unregulated, And Unreported Fishing In The Gulf And Beyond, Madison Landry

LSU Journal of Energy Law and Resources

No abstract provided.

Intestinal Toxicity To Ctla-4 Blockade Driven By Il-6 And Myeloid Infiltration, Yifan Zhou, Yusra B Medik, Bhakti Patel, Daniel B Zamler, Sijie Chen, Thomas Chapman, Sarah Schneider, Elizabeth M Park, Rachel L Babcock, Taylor T Chrisikos, Laura M Kahn, Allison M Dyevoich, Josue E Pineda, Matthew C Wong, Aditya K Mishra, Samuel H Cass, Alexandria P Cogdill, Daniel H Johnson, Sarah B Johnson, Khalida Wani, Debora A Ledesma, Courtney W Hudgens, Jingjing Wang, Md Abdul Wadud Khan, Christine B Peterson, Aron Y Joon, Weiyi Peng, Haiyan S Li, Reetakshi Arora, Ximing Tang, Maria Gabriela Raso, Xuegong Zhang, Wai Chin Foo, Michael T Tetzlaff, Gretchen E Diehl, Karen Clise-Dwyer, Elizabeth M Whitley, Matthew M Gubin, James P Allison, Patrick Hwu, Nadim J Ajami, Adi Diab, Jennifer A Wargo, Stephanie S Watowich

Faculty, Staff and Student Publications

Immune checkpoint blockade (ICB) has revolutionized cancer treatment, yet quality of life and continuation of therapy can be constrained by immune-related adverse events (irAEs). Limited understanding of irAE mechanisms hampers development of approaches to mitigate their damage. To address this, we examined whether mice gained sensitivity to anti-CTLA-4 (αCTLA-4)–mediated toxicity upon disruption of gut homeostatic immunity. We found αCTLA-4 drove increased inflammation and colonic tissue damage in mice with genetic predisposition to intestinal inflammation, acute gastrointestinal infection, transplantation with a dysbiotic fecal microbiome, or dextran sodium sulfate administration. We identified an immune signature of αCTLA-4–mediated irAEs, including colonic neutrophil accumulation …

The Scarabaeoid Beetles Of Maryland (Coleoptera), Dana L. Price, Brett C. Ratcliffe

Zea E-Books Collection

This research recognizes 267 species of scarabaeoid beetles occurring in Maryland. We provide a brief overview of the geology, climate, and vegetation of the state. Keys to the families within Scarabaeoidea occurring in Maryland are provided. We also offer an introduction for each family, keys to all taxa, species descriptions, distributions, and distribution maps for all species, months that adults are observed, notes on their natural history, illustrations, and a glossary of terms. Maryland’s species are found in eight families: Lucanidae (7), Passalidae (1), Geotrupidae (17), Trogidae (18), Ochodaeidae (1), Hybosoridae (2), Glaphyridae (1), and Scarabaeidae (220).

Published as Bulletin …