Digital Commons Network^™

Virology Under The Microscope-A Call For Rational Discourse, Felicia Goodrum, Carolina Lopez, Sean Whelan, Et Al.

2020-Current year OA Pubs

Viruses have brought humanity many challenges: respiratory infection, cancer, neurological impairment and immunosuppression to name a few. Virology research over the last 60+ years has responded to reduce this disease burden with vaccines and antivirals. Despite this long history, the COVID-19 pandemic has brought unprecedented attention to the field of virology. Some of this attention is focused on concern about the safe conduct of research with human pathogens. A small but vocal group of individuals has seized upon these concerns - conflating legitimate questions about safely conducting virus-related research with uncertainties over the origins of SARS-CoV-2. The result has fueled …

The Er Protein Translocation Channel Subunit Sbh1 Controls Virulence Of Cryptococcus Neoformans, Felipe H. Santiago-Tirado, Thomas Hurtaux, Jennifer Geddes-Mcalister, Duy Nguyen, Volkhard Helms, Tamara L Doering, Karin Römisch

2020-Current year OA Pubs

The fungal pathogen Cryptococcus neoformans is distinguished by a cell-wall-anchored polysaccharide capsule that is critical for virulence. Biogenesis of both cell wall and capsule relies on the secretory pathway. Protein secretion begins with polypeptide translocation across the endoplasmic reticulum (ER) membrane through a highly conserved channel formed by three proteins: Sec61, Sbh1, and Sss1. Sbh1, the most divergent, contains multiple phosphorylation sites, which may allow it to regulate entry into the secretory pathway in a species- and protein-specific manner. Absence of

Network Analysis Of Large-Scale Immgen And Tabula Muris Datasets Highlights Metabolic Diversity Of Tissue Mononuclear Phagocytes, Anastasiia Gainullina, Denis A Mogilenko, Li-Hao Huang, Ki-Wook Kim, Karen Krchma, Jun Wu, Geetika Bajpai, Peter Wang, Kyunghee Choi, Kory Lavine, Gwendalyn J Randolph, Alexey Sergushichev, Maxim N Artyomov, Et Al.

2020-Current year OA Pubs

The diversity of mononuclear phagocyte (MNP) subpopulations across tissues is one of the key physiological characteristics of the immune system. Here, we focus on understanding the metabolic variability of MNPs through metabolic network analysis applied to three large-scale transcriptional datasets: we introduce (1) an ImmGen MNP open-source dataset of 337 samples across 26 tissues; (2) a myeloid subset of ImmGen Phase I dataset (202 MNP samples); and (3) a myeloid mouse single-cell RNA sequencing (scRNA-seq) dataset (51,364 cells) assembled based on Tabula Muris Senis. To analyze such large-scale datasets, we develop a network-based computational approach, genes and metabolites (GAM) clustering, …

A Fetal Tumor Suppressor Axis Abrogates Mll-Fusion-Driven Acute Myeloid Leukemia, Mohamed Eldeeb, Ouyang Yuan, Nicola Guzzi, Phuong Cao Thi Ngoc, Anna Konturek-Ciesla, Trine A Kristiansen, Sowndarya Muthukumar, Jeffrey Magee, Cristian Bellodi, Joan Yuan, David Bryder

2020-Current year OA Pubs

MLL-rearrangements (MLL-r) are recurrent genetic events in acute myeloid leukemia (AML) and frequently associate with poor prognosis. In infants, MLL-r can be sufficient to drive transformation. However, despite the prenatal origin of MLL-r in these patients, congenital leukemia is very rare with transformation usually occurring postnatally. The influence of prenatal signals on leukemogenesis, such as those mediated by the fetal-specific protein LIN28B, remains controversial. Here, using a dual-transgenic mouse model that co-expresses MLL-ENL and LIN28B, we investigate the impact of LIN28B on AML. LIN28B impedes the progression of MLL-r AML through compromised leukemia-initiating cell activity and suppression of MYB signaling. …

Does The Potocki-Lupski Syndrome Convey The Autism Spectrum Disorder Phenotype? Case Report And Scoping Review, Oksana I Talantseva, Galina V Portnova, Raisa S Romanova, Daria A Martynova, Olga V Sysoeva, Elena L Grigorenko

Faculty, Staff and Students Publications

Potocki-Lupski Syndrome (PTLS) is a rare condition associated with a duplication of 17p11.2 that may underlie a wide range of congenital abnormalities and heterogeneous behavioral phenotypes. Along with developmental delay and intellectual disability, autism-specific traits are often reported to be the most common among patients with PTLS. To contribute to the discussion of the role of autism spectrum disorder (ASD) in the PTLS phenotype, we present a case of a female adolescent with a de novo dup(17) (p11.2p11.2) without ASD features, focusing on in-depth clinical, behavioral, and electrophysiological (EEG) evaluations. Among EEG features, we found the atypical peak-slow wave patterns …

Unpacking The Proteome And Metaproteome Of The Black Soldier Fly Larvae: Efficacy And Complementarity Of Multiple Protein Extraction Protocols, Utpal Bose, Angela Juhasz, Sally Stockwell, Sophia Escobar-Correas, Anna Marcora, Cate Paull, James A. Broadbent, Gene Wijffels

Research outputs 2022 to 2026

The larvae of the black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), have demonstrated the ability to efficiently bioconvert organic waste into a sustainable source of food and feed, but fundamental biology remains to be discovered to exploit their full biodegradative potential. Herein, LC-MS/MS was used to assess the efficiency of eight differing extraction protocols to build foundational knowledge regarding the proteome landscape of both the BSF larvae body and gut. Each protocol yielded complementary information to improve BSF proteome coverage. Protocol 8 (liquid nitrogen, defatting, and urea/thiourea/chaps) was better than all other protocols for the protein extraction from larvae …

Contribution Of E-Book Implementation To Learning Achievement In Informatics Courses In Antartika, Muhammad Afifuddin Al Islami, Rahmania Sri Untari, Fitria Nur Hasanah, Dicka Y. Kardono

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan

This study aimed to determine (1) the contribution of E-Books to Informatics class X RPL subject to student achievement at SMK Antarctica 1 Sidoarjo (2) How significant is the contribution to student achievement in Informatics class X RPL at SMK Antarctica 1 Sidoarjo after using the E-Book. This research uses a quantitative method with the type of pre-experimental design one research group pretest-posttest design to compare the results obtained from the pretest and posttest. The population used was 36 students, and the sample was taken using a saturated sampling technique, meaning that all population members were taken to be tested. …

Development Of "Kids Learning" Educational Game As A Basic Learning Media About Geometry Materials For Kindergarten Children, Achmad Buchori, Devita Rahmania, Sharifah Osman

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan

The purpose of this research is to increase interest in learning for kindergarten children to recognize android-based flat shapes. This is an instructional game with both a learning and a gameplay menu. There are games on the play option such as Remembering Matches, Counting, and Guessing flat shapes using Multimedia. This study employs the Development Life Cycle (MDLC) model. The targets in this study were students of Kindergarten Mardirini Sidogemah Sayung Demak. Data processing was taken from the validation of media, materials, and user questionnaires using a Likert scale in the assessment. The results of the expert validation showed an …

Development Of Learning Media For Physical Education, Sports, And Health On Basic Football Techniques Based On Articulate Storyline Application, Rika Navi’Atul Khasanah, Hariyoko Hariyoko

Indonesian Journal of Research in Physical Education, Sport, and Health

The purpose of this research is to develop PJOK learning media in the form of interactive multimedia in the form of the Articulate Storyline application. So, it is hoped that through the development of this learning media it can help and make it easier for PJOK teachers in high schools to convey basic football technical material in an interesting way and help students understand basic football technical material easily. This study uses development research methods with data obtained in the form of qualitative and quantitative data. The results showed that the average value obtained from the validation of football learning …

Survey Of Physical Conditions Of Prospective Athletes "Perisai Diri" Malang District Branch, Wahyu Nadia Nur Khasanah, Eko Hariyanto

Indonesian Journal of Research in Physical Education, Sport, and Health

Physical conditions greatly affect the ability and skills of an athlete to master movement in a sport, especially Pencak Silat. The purpose of this research is to find out how the physical condition of the athletes at the level of prospective families of the Perisai Diri branch of Malang Regency is. This study used a descriptive quantitative research design and used survey research methods. The results of the survey research on the physical condition of Cakel (Prospective family level) of the Malang Regency branch of Perisai Diri showed that the average physical condition of the red belt level athletes or …

Greater Grison (Galictis Vittata) Predation Events Upon Paca (Cuniculus Paca) Suggest A Cavity Targeted Hunting Strategy By Greater Grison, Andrew Whitworth

Neotropical Biodiversity

No abstract provided.

The Fourth Industrial Revolution: Opportunities And Challenges To The Future Of Mechanical Engineering, Ulingeta O.L. Mbamba

Tanzania Journal of Engineering and Technology (TJET)

This paper provides a conceptual overview of the developments of the Fourth Industrial Revolution (4IR) and derives challenges and opportunities for the future of the mechanical engineering profession. The paper broadly defines 4IR as the fusion of technologies and interaction across physical, digital and biological aspects, while defining mechanical engineering as "a multi-faceted discipline that encompasses the teaching, practice and guidance of others in the development and application of scientific principles to mechanical systems". The paper looks specifically at developments in the Internet of Things (IoT), blockchain technology, robotics, Big Data analytics, biotechnology, nanotechnology, 3D printing and artificial intelligence and …

Cellulose-Keratin Supramolecular Composite: Conformation By Combined Fourier Transform Spectroscopy And Partial Least Square Regression Coefficient, Ngesa Ezekiel Mushi Dr

Tanzania Journal of Engineering and Technology (TJET)

Keratin and cellulose are important nano components with excellent mechanical and biological properties. However, the preparation route through dissolution in organic solvents to make manmade materials is challenging because of their intensive bonding interaction characteristics. Structural modification of the natural attributes is difficult to maintain after polymer regeneration. A new technique by a green route was devised to dissolve keratin from wool, hair, and feather with cellulose in situ using 1-Butyl-3-methylimidazolium chloride [BMIm] + Cl- ionic liquid as a solvent. The mixture of keratin and cellulose homogeneously and synergistically resulted in a supramolecular composite film through non-derivatized mechanochemical interactions. The …

Jaml Immunotherapy Targets Recently Activated Tumor-Infiltrating Cd8+ T Cells, Simon Eschweiler, Alice Wang, Ciro Ramírez-Suástegui, Adrian Von Witzleben, Yingcong Li, Serena J Chee, Hayley Simon, Monalisa Mondal, Matthew Ellis, Gareth J Thomas, Vivek Chandra, Christian H Ottensmeier, Pandurangan Vijayanand

Faculty, Staff and Student Publications

Junctional adhesion molecule-like protein (JAML) serves as a co-stimulatory molecule in γδ T cells. While it has recently been described as a cancer immunotherapy target in mice, its potential to cause toxicity, specific mode of action with regard to its cellular targets, and whether it can be targeted in humans remain unknown. Here, we show that JAML is induced by T cell receptor engagement, reveal that this induction is linked to cis-regulatory interactions between the CD3D and JAML gene loci. When compared with other immunotherapy targets plagued by low target specificity and end-organ toxicity, we find JAML to be mostly …

Narrative Review Of Single Ventricle: Where Are We After 40 Years?, Antonio F Corno, Tina O Findley, Jorge D Salazar

Faculty, Staff and Student Publications

Background and objective: Key medical and surgical advances have been made in the longitudinal management of patients with "functionally" single ventricle physiology, with the principles of Fontan circulation applied to other complex congenital heart defects. The purpose of this article is to review all of the innovations, starting from fetal life, that led to a change of strategy for single ventricle.

Methods: Our literature review included all full articles published in English language on the Cochrane, MedLine, and Embase with references to "single ventricle" and "univentricular hearts", including the initial history of the treatments for this congenital heart defects as …

Narrative Review Of Single Ventricle: Where Are We After 40 Years?, Antonio F Corno, Tina O Findley, Jorge D Salazar

Faculty, Staff and Student Publications

BACKGROUND AND OBJECTIVE: Key medical and surgical advances have been made in the longitudinal management of patients with "functionally" single ventricle physiology, with the principles of Fontan circulation applied to other complex congenital heart defects. The purpose of this article is to review all of the innovations, starting from fetal life, that led to a change of strategy for single ventricle.

METHODS: Our literature review included all full articles published in English language on the Cochrane, MedLine, and Embase with references to "single ventricle" and "univentricular hearts", including the initial history of the treatments for this congenital heart defects as …

Knowledge Graph Empowered Machine Learning Pipelines For Improved Efficiency, Reusability, And Explainability, Revathy Venkataramanan, Aalap Tripathy, Martin Foltin, Hong Yung Yip, Annmary Justine, Amit Sheth

Publications

Artificial intelligence (AI) pipelines are complex, heavily parameterized, and expensive to execute in terms of time and computational resources. Consequently, it is onerous to run experiments with all possible parameter combinations to achieve an optimal solution. However, these AI experiments can be optimized by recommending relevant parameters to commence the experiments, reducing search space significantly, which can be fine tuned further. The relevant parameters can be identified by observing the metadata of pipelines executed in the past, and the relevant pipeline with relevant parameters can be recommended to the user. Currently, there are various metadata frameworks that automatically record the …

Single Cell Preparations Of Mycobacterium Tuberculosis Damage The Mycobacterial Envelope And Disrupt Macrophage Interactions, Ekansh Mittal, Andrew T Roth, Anushree Seth, Srikanth Singamaneni, Wandy Beatty, Jennifer A Philips

2020-Current year OA Pubs

For decades, investigators have studied the interaction of

Apical Secretory Glycoprotein Complex Contributes To Cell Attachment And Entry By Cryptosporidium Parvum, Marianna E. Akey, Rui Xu, Soumya Ravindran, Lisa Funkhouser-Jones, L. David Sibley

2020-Current year OA Pubs

Cryptosporidium parvum is an enteric pathogen that invades epithelial cells in the intestine, where it resides at the apical surface in a unique epicellular location. Compared with those of related apicomplexan parasites, the processes of host cell attachment and invasion by C. parvum are poorly understood. The streamlined C. parvum genome contains numerous mucin-like glycoproteins, several of which have previously been shown to mediate cell attachment, although the majority are unstudied. Here, we identified the antigens recognized by monoclonal antibody (MAb) 1A5, which stains the apical end of sporozoites and mature merozoites. Immunoprecipitation with MAb 1A5 followed by mass spectrometry …

Excess Folic Acid Intake Increases Dna De Novo Point Mutations, Xuanye Cao, Jianfeng Xu, Ying L Lin, Robert M Cabrera, Qiuying Chen, Chaofan Zhang, John W Steele, Xiao Han, Steven S Gross, Bogdan J Wlodarczyk, James R Lupski, Wei Li, Hongyan Wang, Richard H Finnell, Yunping Lei

Faculty, Staff and Students Publications

No abstract provided.

Mucosal And Systemic Neutralizing Antibodies To Norovirus Induced In Infant Mice Orally Inoculated With Recombinant Rotaviruses, Takahiro Kawagishi, Liliana Sánchez-Tacuba, Ningguo Feng, Veronica P Costantini, Ming Tan, Xi Jiang, Kim Y Green, Jan Vinjé, Siyuan Ding, Harry B Greenberg

2020-Current year OA Pubs

Rotaviruses (RVs) preferentially replicate in the small intestine and frequently cause severe diarrheal disease, and the following enteric infection generally induces variable levels of protective systemic and mucosal immune responses in humans and other animals. Rhesus rotavirus (RRV) is a simian RV that was previously used as a human RV vaccine and has been extensively studied in mice. Although RRV replicates poorly in the suckling mouse intestine, infection induces a robust and protective antibody response. The recent availability of plasmid only-based RV reverse genetics systems has enabled the generation of recombinant RVs expressing foreign proteins. However, recombinant RVs have not …

Can Tilt Be Used To Teach Study Tactics? A Case Study In A Biology Classroom, Anne Jacobs

Perspectives In Learning

Recently, there has been a movement encouraging instructors in higher education to use the Transparency in Teaching and Learning (TILT) framework when designing assignments. This framework helps instructors clarify their expectations and evaluation criteria. Making assignments more transparent may result in greater student success. However, it is less clear how this framework can be applied to classes that use exams as a main method of assessing student learning. One option might be to use a TILTed assignment to introduce students to learning tools to improve their studying. I did this by giving students in an introductory biology class an assignment …

Lanthorn, Vol. 57, No. 24, February 27, 2023, Grand Valley State University

Volume 57, August 1, 2022 - April 10, 2023

Lanthorn is Grand Valley State's student newspaper, published from 1968 to the present.

The Daily Gamecock, Redefining Normal, February 2023, University Of South Carolina, Office Of Student Media

February

No abstract provided.

Coloration Strategies In Sympatric Vietnamese Damselflies, Eva J. Driggs, Seth M. Bybee

Library/Life Sciences Undergraduate Poster Competition 2023

Odonates (dragonflies and damselflies) have evolved vibrant coloration for various reasons. Odonates use color signals to identify their own species from others and potential mates. Some Odonates use blue color to blend into the water while others are only active when their color can best be seen. Different selective pressures will evolve a specific color strategy. Color is a key to understanding the evolutionary pressures for damselflies. Color has not been studied in depth for Vietnamese Odonates, even though Vietnam is a top 15 biodiversity hotspot. We hope to better understand color evolution in Vietnamese damselflies and the role …

Mitigating Zoonotic Disease Threats To Prevent Future Pandemics: A Critical Analysis Of Policy Favoring The Closures Of Wildlife Markets In Latin America, Melany J. Danielson

University of Miami Inter-American Law Review

The Preventing Future Pandemics Act was introduced to mitigate zoonotic disease threats around the world by focusing policy efforts on the closure of wildlife markets that gave rise to COVID–19. This Note challenges the efficacy of wildlife market closure policy by considering cultural, socioeconomic, and legal factors for the existence of wildlife market within megadiverse countries in Latin America. Based on scientific research on the animal-to-human interface and zoonotic disease transmission, this Note suggests effective policy should incorporate a targeted species ban for reservoir species, improved sanitary measures and disease surveillance, and wildlife trafficking prevention. Ultimately, this Note calls for …

Assessment Of Ring-Tailed Lemur Lemur Catta Populations In South-Western Madagascar, Sylvain Randrianjaka, Samantha Calkins, Timothy M. Sefczek, Cynthia L. Frasier, Richard Randriamampionona, Jean Claude Rakotoniaina, Lily-Arison R. De Roland, Andrea L. Baden, Edward E. Louis Jr.

School of Global Integrative Studies: Faculty Publications

Anthropogenic activities are negatively affecting the flora and fauna of Madagascar, including its Endangered flagship lemur species, the ring-tailed lemur Lemur catta. Population numbers at some sites are rapidly declining, yet much of the species’ habitat is insufficiently surveyed. Because widespread population assessments are critical to guiding conservation management strategies, additional data are needed to monitor L. catta population trends and to identify the limits of their geographical range. Here we report survey results confirming the presence of this species at 65 of 83 sites in southern and south-western Madagascar, including three subpopulations that were previously considered likely to …

Differential Impacts Of Cereal And Protein Sources Fed To Pigs After Weaning On Diarrhoea And Faecal Shedding Of Escherichia Coli, Production, And Total Tract Apparent Digestibility, John R. Pluske, Bruce P. Mullan, Jae Cheol Kim, David J. Hampson

Animal production and livestock research articles

Different cereal types, in combination with different protein sources, are fed to pigs after weaning, but their interactions and possible implications are not well researched. In this study, 84 male weaned piglets were used in a 21-day feeding trial to investigate the effects of feeding either medium-grain or long-grain extruded rice or wheat, in a factorial combination with protein sources of either vegetable or animal origin, on postweaning performance, shedding of β–haemolytic Escherichia coli, and the coefficient of total tract apparent digestibility (CTTAD). Pigs fed either rice type performed the same (p > 0.05) as wheat-fed pigs after weaning. The use …

Split And Join: An Efficient Approach For Simulating Stapled Intestinal Anastomosis In Virtual Reality, Di Qi, Suvranu De

Engineering Faculty Articles and Research

Colorectal cancer is a life-threatening disease. It is the second leading cause of cancer-related deaths in the United States. Stapled anastomosis is a rapid treatment for colorectal cancer and other intestinal diseases and has become an integral part of routine surgical practice. However, to the best of our knowledge, there is no existing work simulating intestinal anastomosis that often involves sophisticated soft tissue manipulations such as cutting and stitching. In this paper, for the first time, we propose a novel split and join approach to simulate a side-to-side stapled intestinal anastomosis in virtual reality. We mimic the intestine model using …

Home – No Home: Expatriation, Social Integration And Remote Viewing Of Place. An Expanded Photography Approach, Ioannis Galanopoulos-Papavasileiou

All Works

Home – No Home reflects through expanded photographic artworks and elucidate – through heuristic introspection, auto-ethnography, and critical text analysis – notions of expatriation and social integration, such as home, (Blunt & Dowling 2006; Blunt et al., 2007) home displacement and attachment, nostalgia (Vidler, 1992; Koerner, 2017) and homesickness (Fink et al., 2007). Attached to these concepts are deeper questions rooted in societal discourses about today’s global trends and discourses of expatriation, (Fechter, 2007; Leonard 2010; Walsh, 2010, 2012, 2014; Mathur, 2011; and Kunz, 2016) and the politics of border assertion and control. Is the ability to live …