Digital Commons Network^™

Flood Risk Data For Highway Projects, Nabil Ghalayini

Purdue Road School

This presentation will provide project managers and engineers with a clear understanding of available FEMA Risk MAP flood hazard data to better inform project design. This presentation will broadly define flood risk, review the FEMA flood risk database as a source of risk parameters, contrast the regulatory Special Flood Hazard Area (SFHA) with Risk MAP depth and probability grids, and highlight the variability of risk within the SFHA.

Student Handout - Watch-Think-Write - Pair-Share: Pollinators, Center For Urban Resilience

Module 10: Garden Ecology

No abstract provided.

Powerpoint - Attracting Pollinators, Center For Urban Resilience

Module 10: Garden Ecology

No abstract provided.

Minutes 3-14-2023 | Safety Committee, Megan Meade

Safety Committee Minutes

No abstract provided.

March 2023: Region 10 Emerging Special Pathogen Treatment Center (Resptc) At Providence Sacred Heart Medical Center & Children’S Hospital, Providence - Special Pathogens Program

Special Pathogens Newsletters

REGION 10 SPECIAL PATHOGENS NEWSLETTER

Are Humans Superior To Other Animals?, Harold Herzog

Herzog - Companion Animal Issues

Many nonhuman animals exhibit behaviors that vastly exceed human capacities.
Imitation and social learning occur in species ranging from honey bees to chimpanzees. The evolution of our special capacity to use symbolic language to transmit knowledge made cumulative culture a uniquely human phenomenon.

Spoilage Pseudomonas Survive Common Thermal Processing Schedules And Grow In Emulsified Meat During Extended Vacuum Storage, Samuel C. Watson, Rebecca A. Furbeck, Samodha C. Fernando, Byron D. Chaves, Gary Sullivan

Department of Animal Science: Faculty Publications

Some Pseudomonas species are common meat spoilage bacteria that are often associated with the spoilage of fresh meat. The recently reported ability of these bacteria to also spoil cooked and vacuum packaged meat products has created the need to investigate all potential routes of spoilage they may be able to utilize. The objective of this experiment was to determine if spoilage Pseudomonas spp. survive thermal processing and grow during refrigerated storage under vacuum. Pseudomonas spp. isolates collected from spoiled turkey products were inoculated into a salted and seasoned meat emulsion that was vacuum sealed and thermally treated to final temperatures …

Preview; Groo V. Mont. Eleventh Jud. Dist. Ct.: New Frontiers In Social Media And Specific Personal Jurisdiction, Rachel Parker

Montana Law Review Online

No abstract provided.

Understanding The Effects Of Trenbolone Acetate, Polyamine Precursors, And Polyamines On Proliferation, Protein Synthesis Rates, And The Abundance Of Genes Involved In Myoblast Growth, Polyamine Biosynthesis, And Protein Synthesis In Murine Myoblasts, Laura A. Motsinger, Lillian L. Okamoto, Nikole E. Ineck, Brynne A. Udy, Christopher L. Erickson, Youssef Harraq, Caleb C. Reichhardt, Gordon K. Murdoch, Kara Jean Thornton-Kurth

Animal, Dairy, and Veterinary Science Faculty Publications

Research suggests that androgens increase skeletal muscle growth by modulating polyamine biosynthesis. As such, the objective of this study was to investigate effects of anabolic hormones, polyamine precursors, and polyamines relative to proliferation, protein synthesis, and the abundance of mRNA involved in polyamine biosynthesis, proliferation, and protein synthesis in C2C12 and Sol8 cells. Cultures were treated with anabolic hormones (trenbolone acetate and/or estradiol), polyamine precursors (methionine or ornithine), or polyamines (putrescine, spermidine, or spermine). Messenger RNA was isolated 0.5 or 1, 12, or 24 h post-treatment. The cell type had no effect (p > 0.10) on proliferation, protein synthesis, or …

The Role Of Eco-Tourism In The Environmental Protection Of The Mountains Of Pamirs, Furough Shakarmamadova

USF Tampa Graduate Theses and Dissertations

ABSTRACTThe Central Asian nation of Tajikistan became independent in 1991. Tajikistan is a mountainous country with a great potential for tourism development. Nevertheless, there are limitations to the development of eco-tourism. I conducted my study to investigate the potential for developing eco-tourism in the eastern part of Tajikistan (the Pamirs). My overarching question was how does eco-tourism relate to the protection of the environment in Pamirs and can it improve the quality of people's lives? To answer this question, I focused on identifying the Best Management Practices (BMP) of eco-tourism in the region and what specific policies, if any, exist …

Impact Of Crowding On The Diversity Of Expanding Populations., Carl F. Schreck, Diana Fusco, Yuya Karita, Stephen Martis, Jona Kayser, Marie-Cécilia Duvernoy, Oskar Hallatschek

Manuscripts, Articles, Book Chapters and Other Papers

Crowding effects critically impact the self-organization of densely packed cellular assemblies, such as biofilms, solid tumors, and developing tissues. When cells grow and divide, they push each other apart, remodeling the structure and extent of the population's range. Recent work has shown that crowding has a strong impact on the strength of natural selection. However, the impact of crowding on neutral processes, which controls the fate of new variants as long as they are rare, remains unclear. Here, we quantify the genetic diversity of expanding microbial colonies and uncover signatures of crowding in the site frequency spectrum. By combining Luria-Delbrück …

Noninvasive Electromyometrial Imaging Of Human Uterine Maturation During Term Labor, Hui Wang, Zichao Wen, Wenjie Wu, Zhexian Sun, Zulfia Kisrieva-Ware, Yiqi Lin, Sicheng Wang, Hansong Gao, Haonan Xu, Peinan Zhao, Qing Wang, Alan L Schwartz, Phillip Cuculich, Yong Wang, Et Al.

2020-Current year OA Pubs

Electromyometrial imaging (EMMI) was recently developed to image the three-dimensional (3D) uterine electrical activation during contractions noninvasively and accurately in sheep. Herein we describe the development and application of a human EMMI system to image and evaluate 3D uterine electrical activation patterns at high spatial and temporal resolution during human term labor. We demonstrate the successful integration of the human EMMI system during subjects' clinical visits to generate noninvasively the uterine surface electrical potential maps, electrograms, and activation sequence through an inverse solution using up to 192 electrodes distributed around the abdomen surface. Quantitative indices, including the uterine activation curve, …

Glut1 Is Redundant In Hypoxic And Glycolytic Nucleus Pulposus Cells Of The Intervertebral Disc, Shira N. Johnston, Elizabeth S. Silagi, Vedavathi Madhu, Duc H. Nguyen, Irving M. Shapiro, Makarand V. Risbud

Department of Orthopaedic Surgery Faculty Papers

Glycolysis is central to homeostasis of nucleus pulposus (NP) cells in the avascular intervertebral disc. Since the glucose transporter, GLUT1, is a highly enriched phenotypic marker of NP cells, we hypothesized that it is vital for the development and postnatal maintenance of the disc. Surprisingly, primary NP cells treated with 2 well-characterized GLUT1 inhibitors maintained normal rates of glycolysis and ATP production, indicating intrinsic compensatory mechanisms. We showed in vitro that NP cells mitigated GLUT1 loss by rewiring glucose import through GLUT3. Of note, we demonstrated that substrates, such as glutamine and palmitate, did not compensate for glucose restriction resulting …

T-Cell Receptor Repertoire Sequencing In The Era Of Cancer Immunotherapy, Meredith L Frank, Kaylene Lu, Can Erdogan, Yi Han, Jian Hu, Tao Wang, John V Heymach, Jianjun Zhang, Alexandre Reuben

Faculty, Staff and Student Publications

T cells are integral components of the adaptive immune system, and their responses are mediated by unique T-cell receptors (TCR) that recognize specific antigens from a variety of biological contexts. As a result, analyzing the T-cell repertoire offers a better understanding of immune responses and of diseases like cancer. Next-generation sequencing technologies have greatly enabled the high-throughput analysis of the TCR repertoire. On the basis of our extensive experience in the field from the past decade, we provide an overview of TCR sequencing, from the initial library preparation steps to sequencing and analysis methods and finally to functional validation techniques. …

Genetic Architecture Of Spatial Electrical Biomarkers For Cardiac Arrhythmia And Relationship With Cardiovascular Disease, William J Young, Jeffrey Haessler, Jan-Walter Benjamins, Linda Repetto, Jie Yao, Aaron Isaacs, Andrew R Harper, Julia Ramirez, Sophie Garnier, Stefan Van Duijvenboden, Antoine R Baldassari, Maria Pina Concas, Thuyvy Duong, Luisa Foco, Jonas L Isaksen, Hao Mei, Raymond Noordam, Casia Nursyifa, Anne Richmond, Meddly L Santolalla, Colleen M Sitlani, Negin Soroush, Sébastien Thériault, Stella Trompet, Stefanie Aeschbacher, Fariba Ahmadizar, Alvaro Alonso, Jennifer A Brody, Archie Campbell, Adolfo Correa, Dawood Darbar, Antonio De Luca, Jean-François Deleuze, Christina Ellervik, Christian Fuchsberger, Anuj Goel, Christopher Grace, Xiuqing Guo, Torben Hansen, Susan R Heckbert, Rebecca D Jackson, Jan A Kors, Maria Fernanda Lima-Costa, Allan Linneberg, Peter W Macfarlane, Alanna C Morrison, Pau Navarro, David J Porteous, Peter P Pramstaller, Alexander P Reiner, Lorenz Risch, Ulrich Schotten, Xia Shen, Gianfranco Sinagra, Elsayed Z Soliman, Monika Stoll, Eduardo Tarazona-Santos, Andrew Tinker, Katerina Trajanoska, Eric Villard, Helen R Warren, Eric A Whitsel, Kerri L Wiggins, Dan E Arking, Christy L Avery, David Conen, Giorgia Girotto, Niels Grarup, Caroline Hayward, J Wouter Jukema, Dennis O Mook-Kanamori, Morten Salling Olesen, Sandosh Padmanabhan, Bruce M Psaty, Cristian Pattaro, Antonio Luiz P Ribeiro, Jerome I Rotter, Bruno H Stricker, Pim Van Der Harst, Cornelia M Van Duijn, Niek Verweij, James G Wilson, Michele Orini, Philippe Charron, Hugh Watkins, Charles Kooperberg, Henry J Lin, James F Wilson, Jørgen K Kanters, Nona Sotoodehnia, Borbala Mifsud, Pier D Lambiase, Larisa G Tereshchenko, Patricia B Munroe

Faculty, Staff and Student Publications

The 3-dimensional spatial and 2-dimensional frontal QRS-T angles are measures derived from the vectorcardiogram. They are independent risk predictors for arrhythmia, but the underlying biology is unknown. Using multi-ancestry genome-wide association studies we identify 61 (58 previously unreported) loci for the spatial QRS-T angle (N = 118,780) and 11 for the frontal QRS-T angle (N = 159,715). Seven out of the 61 spatial QRS-T angle loci have not been reported for other electrocardiographic measures. Enrichments are observed in pathways related to cardiac and vascular development, muscle contraction, and hypertrophy. Pairwise genome-wide association studies with classical ECG traits identify shared genetic …

Creating The Listener Language: A Constructed Language With Semantic Rhythms And Tones, Dallyn H. Mccracken

Undergraduate Honors Theses

Through creating a language that uses semantic rhythms to convey information in ways that typical intonation does not, I was able to see the benefits and drawbacks of strict semantic intonation. The Listener language replaces intonation with rhythms, and it uses rhythms in place of intonation in cases where intonation would generally be used in other languages, though it also uses rhythms in all other speech. Rhythms in the Listener language are grammatical, and speaking the language without the rhythms is considered to be ungrammatical. During the language construction process, there were several setbacks, both expected and unexpected. The Listener …

Large And Stable Genome Edits At The Sorghum Alpha Kafirin Locus Result In Changes In Chromatin Accessibility And Globally Increased Expression Of Genes Encoding Lysine Enrichment, J. Preston Hurst, Abou Yobi, Aixia Li, Shirley Sato, Thomas E. Clemente, Ruthie Angelovici

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sorghum is a resilient and widely cultivated grain crop used for feed and food. However, it’s grain is deficient in lysine, an essential amino acid. This is due to the primary seed storage proteins, the alpha-kafirins, lacking lysine. It has been observed that reductions in alpha-kafirin protein results in rebalancing of the seed proteome and a corresponding increase in non-kafirin proteins which leads to an increased lysine content. However, the mechanisms underlying proteome rebalancing are unclear. This study characterizes a previously developed gene edited sorghum line, with deletions at the alpha kafirin locus.

Methods: A single consensus …

Foregrounding The Code: Computational Chemistry Instructional Activities Using A Highly Readable Fluid Simulation Code, Gianmarc Grazioli, Adam Ingwerson, David Santiago, Patrick Regan, Hee Kun Cho

Faculty Research, Scholarly, and Creative Activity

Computational chemistry instructional activities are often based around students running chemical simulations via a graphical user interface (GUI). GUI-based activities offer many advantages, as they enable students to run chemical simulations with a few mouse clicks. Although these activities are excellent for introducing students to the capabilities of chemical simulations, the disadvantage is that the students’ experience is not representative of how professional computational chemists work. Just as it is important that students in an organic chemistry instructional lab gain hands-on experience with equipment commonly used by professional organic chemists, students of computational chemistry must gain hands-on experience with coding, …

Diatom Diversity And Functional Groups In 72 Florida Lakes: Assessing Ecological Changes For Improved Protection And Management, Francesca M. Lauterman

USF Tampa Graduate Theses and Dissertations

Florida lakes are diverse ecosystems and are an important part of the state's biodiversity and ecosystem services. As human population and development increase across the state, many lakes have been documented as nutrient impaired and are unable to maintain essential ecological functions. In this study, diatom diversity and functional groups in 72 Florida lakes were assessed for their relationships with associated water-quality data. Common alpha diversity indices, including Shannon-Weiner Index, the Gini-Simpson Index, and Hill’s numbers were utilized to measure trends in diatom communities, while the relationships between diatom lifeform characteristics and environmental data were explored using multivariate methods and …

Renal Cell Carcinoma Unclassified With Medullary Phenotype In A Patient With Neurofibromatosis Type 2, Sanila Sarkar, Whitney Throckmorton, Racheal Bingham, Pavlos Msaouel, Giannicola Genovese, John Slopis, Priya Rao, Zsila Sadighi, Cynthia E Herzog

Faculty, Staff and Student Publications

We present, to our knowledge, the first reported case of germline neurofibromatosis Type 2 (NF2) associated with renal cell carcinoma unclassified with medullary phenotype (RCCU-MP) with somatic loss by immunohistochemistry of the SMARCB1 tumor suppressor gene located centromeric to NF2 on chromosome 22q. Our patient is a 15-year-old with germline neurofibromatosis Type 2 (NF2) confirmed by pathogenic mutation of c.-854-??46+??deletion. Her NF2 history is positive for a right optic nerve sheath meningioma, CNIII schwannoma requiring radiation therapy and post gross total resection of right frontotemporal anaplastic meningioma followed by radiation. At age 15 she developed new onset weight loss and …

Machine Intelligence Framework For Predictive Modeling Of Co2 Concentration: A Path To Sustainable Environmental Management, Mohamed Abouhawwash, Mohammed Jameel, Sameh S. Askar

Sustainable Machine Intelligence Journal

Climate change poses critical challenges, necessitating accurate and timely monitoring of CO2 concentrations for sustainable environmental management. Traditional methods for CO2 prediction exhibit limitations in precision and scalability. This paper introduces a novel Machine Intelligence Framework (MIF) specifically designed for predictive modeling of CO2 concentration levels. Leveraging advanced machine learning algorithms and data processing techniques, MIF aims to address the existing research gap by offering enhanced accuracy and adaptability in CO2 forecasting. Motivated by the urgency to combat climate change, this research develops a comprehensive framework integrating predictive modeling with machine intelligence. The methodology involves algorithm design, data integration, and …

Acute Acat1/Soat1 Blockade Increases Mam Cholesterol And Strengthens Er-Mitochondria Connectivity., Taylor C Harned, Radu V Stan, Ze Cao, Rajarshi Chakrabarti, Henry N Higgs, Catherine C Y Chang, Ta Yuan Chang

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Cholesterol is a key component of all mammalian cell membranes. Disruptions in cholesterol metabolism have been observed in the context of various diseases, including neurodegenerative disorders such as Alzheimer's disease (AD). The genetic and pharmacological blockade of acyl-CoA:cholesterol acyltransferase 1/sterol O-acyltransferase 1 (ACAT1/SOAT1), a cholesterol storage enzyme found on the endoplasmic reticulum (ER) and enriched at the mitochondria-associated ER membrane (MAM), has been shown to reduce amyloid pathology and rescue cognitive deficits in mouse models of AD. Additionally, blocking ACAT1/SOAT1 activity stimulates autophagy and lysosomal biogenesis; however, the exact molecular connection between the ACAT1/SOAT1 blockade and these observed benefits remain …

Shedding Light On Mechanisms Of Myocarditis With Covid-19 Mrna Vaccines, Biykem Bozkurt

Faculty, Staff and Students Publications

No abstract provided.

Human Schistosomiasis Vaccines As Next Generation Control Tools, Peter J Hotez, Maria Elena Bottazzi

Faculty, Staff and Students Publications

No abstract provided.

Tsks Localizes To Nuage In Spermatids And Regulates Cytoplasmic Elimination During Spermiation, Keisuke Shimada, Soojin Park, Seiya Oura, Taichi Noda, Akane Morohoshi, Martin M Matzuk, Masahito Ikawa

Faculty, Staff and Students Publications

Spermatozoa have a streamlined shape to swim through the oviduct to fertilize oocytes. To become svelte spermatozoa, spermatid cytoplasm must be eliminated in several steps including sperm release, which is part of spermiation. Although this process has been well observed, the molecular mechanisms that underlie it remain unclear. In male germ cells, there are membraneless organelles called nuage, which are observed by electron microscopy in various forms of dense material. Reticulated body (RB) and chromatoid body remnant (CR) are two types of nuage in spermatids, but the functions of both are unknown. Using CRISPR/Cas9 technology, we deleted the entire coding …

In Vivo Functional Characterization Of Egfr Variants Identifies Novel Drivers Of Glioblastoma, Kwanha Yu, Kathleen Kong, Brittney Lozzi, Estefania Luna-Figueroa, Alexis Cervantes, Rachel Curry, Carrie A Mohila, Ganesh Rao, Ali Jalali, Gordon B Mills, Kenneth L Scott, Benjamin Deneen

Faculty, Staff and Students Publications

BACKGROUND: Glioblastoma is the most common and aggressive primary brain tumor. Large-scale sequencing initiatives have cataloged its mutational landscape in hopes of elucidating mechanisms driving this deadly disease. However, a major bottleneck in harnessing this data for new therapies is deciphering "driver" and "passenger" events amongst the vast volume of information.

METHODS: We utilized an autochthonous, in vivo screening approach to identify driver, EGFR variants. RNA-Seq identified unique molecular signatures of mouse gliomas across these variants, which only differ by a single amino acid change. In particular, we identified alterations to lipid metabolism, which we further validated through an unbiased …

Slow Looking: Powerful Tool Of Mindfulness To Facilitate Transfer, Young Kyung Min

Journal of Contemplative Inquiry

This paper illustrates how teaching through a “slow looking” pedagogy helps students of Generation Z—who have grown up in a culture of distraction and multitasking—cultivate the habits of mind and dispositions that can enhance their academic performance as well as physical and mental health during pandemic remote learning. Drawing on students’ practice of slow looking in freshman writing courses, the paper demonstrates the power of slow looking as a tool of mindfulness to develop students’ insights and knowledge that are useful in educational settings as well as in their personal lives. It elucidates how their practices of slow looking in …

Dialogic Reciprocity And Contemplative Mentorship, Beth Connors-Manke

Journal of Contemplative Inquiry

While widely recognized as an important part of education, mentorship is rarely taught as a pedagogical practice. In my article, I theorize mentorship philosophically and contemplatively by considering Martin Buber’s (1937) understanding of the I-Thou meeting. I also offer a taxonomy of mentorship, arguing that mentorship can recognize students’ personhood and prefigure the I-Thou encounter. My final sections offer praxis-oriented discussions on listening and grading as a way to prepare students for intersubjective encounters.

Poetry As A Contemplative Pedagogical Practice, Neill Korobov

Journal of Contemplative Inquiry

This reflection argues for the integration of poetry into our teaching as part of a contemplative orientation to pedagogy. I discuss poetry both as a way of awakening a relationship with truth that lies at the periphery of our awareness and as a form of language that disarms us and opens the way to a robust form of vulnerability and courage. The poem “Living Together” by David Whyte is explored to illustrate the ways poetry invites us to think about teaching as a unique type of apprenticeship, as a call to maturity and love, and as a unique form of …

Journeying Home Through The Body: An Introduction To Resource, Resilience, And Resistance In Relationship (R4), Dominique A. Malebranche

Journal of Contemplative Inquiry

This paper will explore the connections to Black cultural reclamation, liberatory healing, and future building through a framework of cultivating a sense of home in contemplative body-based Resource, Resilience, and Resistance in Relationship (R4). Home is a concept that has been historically disrupted for Black and Brown bodies across generations. As people of African descent, we carry stories of homegoing, stolen histories, and politicized futures. Displaced from our motherlands and separated from our resources, home is lost. In a society where we are racialized and subjectively treated and mistreated by the rigidity of white cisheteropatriarchal supremacy, home is needed. Through …