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In Vitro Dissolution And Permeability Testing Of Inhalation Products: Challenges And Advances., Ali Nokhodchi, Salonee Chavan, Taravat Ghafourian

HPD Articles

In vitro dissolution and permeability testing aid the simulation of the in vivo behavior of inhalation drug products. Although the regulatory bodies have specific guidelines for the dissolution of orally administered dosage forms (e.g., tablets and capsules), this is not the case for orally inhaled formulations, as there is no commonly accepted test for assessing their dissolution pattern. Up until a few years ago, there was no consensus that assessing the dissolution of orally inhaled drugs is a key factor in the assessment of orally inhaled products. With the advancement of research in the field of dissolution methods for orally …

Evaluation Of Peroxyacetic Acid, Liquid Buffered Vinegar, And Cultured Dextrose Fermentate As Potential Antimicrobial Interventions For Raw Chicken Livers, Leslie Pearl M. Cancio, Mary-Grace C. Danao, Gary Sullivan, Byron D. Chaves

Department of Nutrition and Health Sciences: Faculty Publications

This study aimed to evaluate the use of peroxyacetic acid (PAA), buffered vinegar (BV), and cultured dextrose fermentate (CDF) to reduce Salmonella on artificially inoculated raw chicken livers, one of the most consumed offal around the world. Samples were inoculated with a 5-strain cocktail of poultry-borne Salmonella to obtain 10⁶ CFU/g and immersed for 90 s with agitation in one of the following treatments: distilled water (control), 450 ppm PAA, 2.0% (w/v) BV, or 1.5% (w/v) CDF, prior to storing at 4^oC. Salmonella was enumerated on XLD agar and monitored for 14 days. Data were analyzed using …

Serum And Urinary Soluble Α-Klotho As Markers Of Kidney And Vascular Impairment, Julia Martín-Vírgala, Adriana S Dusso, Et Al.

2020-Current year OA Pubs

This study was designed to investigate the controversy on the potential role of sKlotho as an early biomarker in Chronic Kidney Disease-Mineral Bone Disorder (CKD-MBD), to assess whether sKlotho is a reliable marker of kidney α-Klotho, to deepen the effects of sKlotho on vascular smooth muscle cells (VSMCs) osteogenic differentiation and to evaluate the role of autophagy in this process. Experimental studies were conducted in CKD mice fed a normal phosphorus (CKD+NP) or high phosphorus (CKD+HP) diet for 14 weeks. The patients' study was performed in CKD stages 2-5 and in vitro studies which used VSMCs exposed to non-calcifying medium …

Endogenous But Not Sensory-Driven Activity Controls Migration, Morphogenesis And Survival Of Adult-Born Juxtaglomerular Neurons In The Mouse Olfactory Bulb, Kaizhen Li, Katherine Figarella, Xin Su, Yury Kovalchuk, Jessika Gorzolka, Jonas J Neher, Nima Mojtahedi, Nicolas Casadei, Ulrike B S Hedrich, Olga Garaschuk

Faculty, Staff and Student Publications

The development and survival of adult-born neurons are believed to be driven by sensory signaling. Here, in vivo analyses of motility, morphology and Ca²⁺ signaling, as well as transcriptome analyses of adult-born juxtaglomerular cells with reduced endogenous excitability (via cell-specific overexpression of either Kv1.2 or Kir2.1 K⁺ channels), revealed a pronounced impairment of migration, morphogenesis, survival, and functional integration of these cells into the mouse olfactory bulb, accompanied by a reduction in cytosolic Ca²⁺ fluctuations, phosphorylation of CREB and pCREB-mediated gene expression. Moreover, K⁺ channel overexpression strongly downregulated genes involved in neuronal migration, differentiation, and morphogenesis …

Virulence Factors Of Helicobacter Pylori, Haifaa B. Najee, Shaimaa M.S. Zainulabdeen

Muthanna Medical Journal

One of the most common cancers in both genders, gastric cancer is currently the fourth leading cause of cancer-related deaths globally. The interplay of hereditary and environmental variables, including Helicobacter pylori (H. pylori) infection, is linked to the etiology of stomach cancer. Due to a number of evasive mechanisms brought on by the virulence factors that the bacteria expresses, the invasion, survival, colonization, and stimulation of further inflammation within the gastric mucosa are all conceivable. To improve eradication efforts and stop the potential induction of carcinogenesis, it is essential to understand the pathogenicity mechanisms of H. pylori. This review focuses …

The Mediating Role Of Positive Attitudes On The Relationship Between Esports Gaming Hours And Psychological Well-Being During The Covid-19 Pandemic, Dan Shan, Zhihao Dai, Fenfen Ge, Yanyi Zhang, Yuandian Zheng, Xiaoyi Gao, Junchu Han

Student Publications

Electronic sports game (esports) gaming has seen a surge in popularity, especially during the coronavirus disease 2019 (COVID-19) pandemic, with more young people turning to it as an alternative to physical activities. However, the impact of esports gaming on mental health is a matter of concern. Previous studies have produced inconsistent findings on the relationship between gaming hours and mental health, and the moderating factors involved remain unexplored. This study aimed to investigate the moderating effect of participants' subjective attitudes toward esports gaming on the relationship between daily gaming hours and psychological well-being (PWB) among Chinese young adults during the …

Morphological Differences Align With Habitat Partitioning Among Three Species Of Percina (Percidae: Actinopterygii) In The Roanoke River, Virginia, Michael M. Calvert, Steven L. Powers

Southeastern Fishes Council Proceedings

The upper Roanoke River has three species of Percina (P. nevisense, Chainback Darter; P. roanoka, Roanoke Darter; and P. rex, Roanoke Logperch). Resource partitioning appears to be a key component of maintaining diverse fish assemblages with habitat and food partitioning cited as especially important in communities containing members of the same family. Some aspects of the diets of these species have been documented in the literature with only modest differences among them. Microhabitat data for adults of these species have also been published revealing differences in habitat occupied by each with P. roanoka living in the fastest, shallowest …

Impacted Communities Leading Authentic Legal Mobilization: A Refugee-Led Access-To-Justice Story, Douglas Smith

Journal of Civil Rights and Economic Development

(Excerpt)

I have a modest proposal to begin addressing the civil access-to-justice problem in the United States: eliminate the barriers for refugees to provide legal representation. In discussions of access to civil justice, immigration and immigrant rights compel our attention—images of children as young as three facing deportation without representation and non-citizens detained because of civil immigration infractions come to mind. But we hear less about the access-to-justice challenges of immigrants fighting for their rights to safe housing, public benefits, education for their children, or often-contingent or under-the-table jobs. The cries of immigrant communities about informal and formal threats from …

The Beacon, March 17, 2023, Beacon Staff

The Beacon, 2022-2023

Unsung Heroes: Sherri Langton; Women's History Month at NW; Paramore makes triumphant return; "Cocaine Bear" movie laced with let-downs; Spreading the gospel through theatre; NW concert band shares gift of music on tour; NW students have an opportunity to learn Latin; Langton embraces community in her role at NW; Whodunnit comes to Fern for a second year; Women's History Month; Reverse Amish Rumspringa; The Great Northwestern Rebrand; NW Cheer Team Ends on a High Note in GPAC; No. 17 Raiders Hot at Tucson Invitational; Raider Intermural Basketball Champions: Tun-A-Sub; TEDx at NW to spark hope through ideas; NW students serve …

Allostatic Load In Opioid Use Disorder: A Scoping Review Protocol., Jaimie Qiuyun Fan, Hillary Miller, Amanda Adams, Rebecca Bryan, Matthew Salzman

Cooper Medical School of Rowan University Departmental Research

INTRODUCTION: Opioid use disorder affects 2.1 million individuals in the USA, causing more than 100 000 overdose-related deaths annually. While the neurobiological model of addiction is well described and accepted, there is a lack of morbidity and mortality prognosticators for patients struggling with opioid use disorder. Allostatic load index is a promising candidate for the basis of a prognostication tool. Previous studies show that allostatic load predicts both morbidity and mortality in a variety of cohorts. This scoping review protocol provides the rationale and steps for summarizing and presenting existing evidence surrounding allostatic load in the context of opioid use …

The Hermeneutics Of Nudging: The Reciprocity Between Transhumanism And Nudging, Ian Doherty

Graduate Student Research Symposium

This paper investigates and evaluates the implications of nudging someone toward transhumanism. Transhumanism offers a path to alleviate suffering and transcend our physical and mental limitations. Transhumanist technologies consist of alterations like genetic modification, neural implants, and molecular nanotechnology. These technologies are meant to give people full morphological freedom over their bodies. Although transhumanism remains a largely unknown movement, this is where nudge theory can help raise its prominence. Nudge theory aims to help people make better choices, and aid them in making better decisions related to their health and lifestyle. If paired correctly, transhumanism seems to be a perfect …

Targeted Delivery Of Therapeutic Agents To The Mouse Brain Using A Stereotactic-Guided Focused Ultrasound Device, Zhongtao Hu, Yaoheng Yang, Dezhuang Ye, Si Chen, Yan Gong, Chinwendu Chukwu, Hong Chen

2020-Current year OA Pubs

Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).

An Analysis Of The Oil Pollution Act Of 1990: Regulatory Calibration In Anticipation Of 21st Century Environmental Disasters, Jessica S. Thornton

USF Tampa Graduate Theses and Dissertations

After federal the Oil Pollution Act of 1990 (OPA 90) was promulgated in response to the Exxon Valdez oil spill in Alaska, it helped restructure the nation’s environmental response capabilities and modernize the commercial shipping industry to effectively decrease environmental shipping disasters. However, in the 33 years since OPA 90’s inception, the oil and gas industry has transformed, especially in the offshore realm, as industry adopts new drilling technologies to gain access to deeper and heretofore untapped maritime environments. Though there have been two catastrophic offshore drilling accidents since the turn of the 21st century, OPA 90 remains categorically unchanged, …

Toward Efficient Rendering: A Neural Network Approach, Qiqi Hou

Dissertations and Theses

Physically-based image synthesis has attracted considerable attention due to its wide applications in visual effects, video games, design visualization, and simulation. However, obtaining visually satisfactory renderings with ray tracing algorithms often requires casting a large number of rays and thus takes a vast amount of computation. The extensive computational and memory requirements of ray tracing methods pose a challenge, especially when running these rendering algorithms on resource-constrained platforms, and impede their applications that require high resolutions and refresh rates. This thesis presents three methods to address the challenge of efficient rendering.

First, we present a hybrid rendering method to speed …

Gemc1 And Mcidas Interactions With Swi/Snf Complexes Regulate The Multiciliated Cell-Specific Transcriptional Program, Michael Lewis, Tao Cheng, Moe R Mahjoub, Et Al.

2020-Current year OA Pubs

Multiciliated cells (MCCs) project dozens to hundreds of motile cilia from their apical surface to promote the movement of fluids or gametes in the mammalian brain, airway or reproductive organs. Differentiation of MCCs requires the sequential action of the Geminin family transcriptional activators, GEMC1 and MCIDAS, that both interact with E2F4/5-DP1. How these factors activate transcription and the extent to which they play redundant functions remains poorly understood. Here, we demonstrate that the transcriptional targets and proximal proteomes of GEMC1 and MCIDAS are highly similar. However, we identified distinct interactions with SWI/SNF subcomplexes; GEMC1 interacts primarily with the ARID1A containing …

Internal Capsule Microstructure Mediates The Relationship Between Childhood Maltreatment And Ptsd Following Adulthood Trauma Exposure, Samantha A. Wong, Stacey L. House, Deanna M. Barch, Et Al.

2020-Current year OA Pubs

No abstract provided.

Global Scientific Trends On Healthy Eating From 2002 To 2021: A Bibliometric And Visualized Analysis, Te Fang, Hongyi Cao, Yue Wang, Yang Gong, Zhongqing Wang

Faculty, Staff and Student Publications

Diet has been recognized as a vital risk factor for non-communicable diseases (NCDs), climate changes, and increasing population, which has been reflected by a rapidly growing body of the literature related to healthy eating. To reveal a panorama of the topics related to healthy eating, this study aimed to characterize and visualize the knowledge structure, hotspots, and trends in this field over the past two decades through bibliometric analyses. Publications related to healthy eating between 1 January 2002 and 31 December 2021 were retrieved and extracted from the Web of Science database. The characteristics of articles including publication years, journals, …

Effects Of The Ketogenic Diet On Spatial Memory And Long-Term Potentiation In The Ca1 Region Of The Hippocampus In Young Rodents, Jed Christensen

Undergraduate Honors Theses

The ketogenic diet (KD) originated as a treatment for epilepsy nearly a century ago, though its neurological effects are not completely understood. In recent years the diet has resurged as a weight loss aid, reinvigorating research investigating its effects on the nervous system. Notably, the KD alters the concentration of glucose and ketones in the brain, and recent research suggests that the elevated ketone concentration, in the form of beta-hydroxybutyrate, induced by the diet enhances recognition memory and mitochondrial efficiency in the hippocampus. To further explore the effects of the KD, we examined its cognitive effects on spatial memory and …

Trapped By The Past: A History And Translation Of An Equatoguinean Poet, Paul A. Guajardo

Undergraduate Honors Theses

Atrapado por el pasado (Trapped by the Past in English) is a collection of Spanish-language poetry and prose by the Equatoguinean poet Zankús Mázé Menemádjimol. Menemádjimol’s work has never received an English translation, and this thesis provides a poetic rendition of three of his poems. It also offers a brief historical introduction, literary overview, and commentary on the poems. While the main goal of this project is artistic and creative, it also aims to introduce readers to an oft-overlooked culture. Equatorial Guinea and the poet’s home island of Annobón are frequently ignored in the fields of both literature and history. …

Masterchef Contestant To Social Media Influencer: Do Men And Women Differ In Pursuing Prestige?, Jada Thomas, Robert Deaner

Student Summer Scholars Manuscripts

The male show-off hypothesis claims that men are more likely than women to show off their abilities and publicly compete. However, the male show-off hypothesis has yet to be tested in a domain where both men and women are consistently involved, such as cooking. To examine showing off and cooking, we assessed the social media behavior of MasterChef reality program contestants both in the U.S. and Australia. We focused on 98 contestants from MasterChef U.S. (m = 52 & f = 46) and 107 contestants from MasterChef Australia (m = 45 & f = 62). For each contestant, we examined …

Atlas Of Ticks (Acari: Argasidae, Ixodidae) In Germany: 1st Data Update, Franz Rubel, Stefan Zaenker, Alexander Weigand, Dieter Weber, Lidia Chitimia-Dobler, Olaf Kahl

Harold W. Manter Laboratory of Parasitology: Library Materials

The first data update of the atlas of ticks in Germany published in 2021 is presented here. This atlas provides maps based on georeferenced tick locations of 21 species endemic in Germany as well as three tick species that are regularly imported to Germany. The data update includes the following numbers of newly georeferenced tick locations: 17 Argas reflexus, 79 Carios vespertilionis, 2 Dermacentor marginatus, 43 Dermacentor reticulatus, 4 Haemaphysalis concinna, 3 Haemaphysalis punctata, 3 Hyalomma rufipes, 3 Ixodes apronophorus, 9 Ixodes arboricola, 1 Ixodes ariadnae, 30 Ixodes canisuga, …

An Intelligent Decision Support Model For Optimal Selection Of Machine Tool Under Uncertainty: Recent Trends, Ibrahim M. Hezam

Neutrosophic Systems with Applications

Many scholars have been interested in the subject of machine tool selection as a result of the growing number of different machines and the continuous advancement of technology associated with these machines. The selection of an unsuitable machine tool may lead to a variety of issues, including limitations on production capacities and productivity indicators when taking into account both time and money from an industrial and practical perspective. The present strategy of selecting machine tools, known as multi-criteria decision-making (MCDM), relies on the subjective viewpoint the vast majority of the time. When selecting an appropriate machining tool, however, it is …

A Mast Cell-Thermoregulatory Neuron Circuit Axis Regulates Hypothermia In Anaphylaxis, Chunjing Bao, Ouyang Chen, Huaxin Sheng, Jeffrey Zhang, Yikai Luo, Byron W Hayes, Han Liang, Wolfgang Liedtke, Ru-Rong Ji, Soman N Abraham

Faculty, Staff and Student Publications

IgE-mediated anaphylaxis is an acute life-threatening systemic reaction to allergens, including certain foods and venoms. Anaphylaxis is triggered when blood-borne allergens activate IgE-bound perivascular mast cells (MCs) throughout the body, causing an extensive systemic release of MC mediators. Through precipitating vasodilatation and vascular leakage, these mediators are believed to trigger a sharp drop in blood pressure in humans and in core body temperature in animals. We report that the IgE/MC-mediated drop in body temperature in mice associated with anaphylaxis also requires the body's thermoregulatory neural circuit. This circuit is activated when granule-borne chymase from MCs is deposited on proximal TRPV1+ …

A Differentiation Roadmap Of Murine Placentation At Single-Cell Resolution, Xiangxiang Jiang, Yue Wang, Zhenyu Xiao, Long Yan, Shanshan Guo, Yiming Wang, Hao Wu, Xuehan Zhao, Xiaoyin Lu, Hongmei Wang

Faculty, Staff and Student Publications

The placenta is one of the most important yet least understood organs. Due to the limitations of conventional research approaches, we are still far from a comprehensive understanding of mouse placentation, especially regarding the differentiation of trophoblast lineages at the early developmental stage. To decipher cell compositions and developmental processes, we systematically profile the single-cell transcriptomes of trophoblast cells from extraembryonic tissues (embryonic day 7.5 (E7.5) and E8.5) and placentae (E9.5-E14.5) at one-day intervals. We identify distinct trophoblast cell types during mouse placentation, including unreported progenitor cells and intermediate precursor cells. An updated differentiation roadmap of mouse trophoblast lineages is …

Ferroptosis Hijacking By Mycobacterium Tuberculosis, Boyi Gan

Faculty, Staff and Student Publications

A recent study from Nature Communications reveals that Mycobacterium tuberculosis can hijack epigenetic machinery in host cells and induce host cell ferroptosis, which promotes pathogen pathogenicity and spread. These findings also suggest new therapeutic strategies to treat tuberculosis.

A New Era Of Morphological Investigations: Reviewing Methods For Comparative Anatomical Studies, K. L. Ford, J. S. Albert, A. P. Summers, B. P. Hedrick, E. R. Schachner, A. S. Jones, K. Evans, P. Chakrabarty

School of Medicine Faculty Publications

The increased use of imaging technology in biological research has drastically altered morphological studies in recent decades and allowed for the preservation of important collection specimens alongside detailed visualization of bony and soft-tissue structures. Despite the benefits associated with these newer imaging techniques, there remains a need for more "tra- ditional"methods of morphological examination in many comparative studies. In this paper, we describe the costs and benefits of the various methods of visualizing, examining, and comparing morphological structures. There are significant differences not only in the costs associated with these different methods (monetary, time, equipment, and software), but also in …

Imaging Of Brain Structural And Functional Effects In People With Human Immunodeficiency Virus, Erin E O'Connor, Edith V Sullivan, Linda Chang, Dima A Hammoud, Tony W Wilson, Ann B Ragin, Christina S Meade, Jennifer Coughlin, Beau M Ances

2020-Current year OA Pubs

Before the introduction of antiretroviral therapy, human immunodeficiency virus (HIV) infection was often accompanied by central nervous system (CNS) opportunistic infections and HIV encephalopathy marked by profound structural and functional alterations detectable with neuroimaging. Treatment with antiretroviral therapy nearly eliminated CNS opportunistic infections, while neuropsychiatric impairment and peripheral nerve and organ damage have persisted among virally suppressed people with HIV (PWH), suggesting ongoing brain injury. Neuroimaging research must use methods sensitive for detecting subtle HIV-associated brain structural and functional abnormalities, while allowing for adjustments for potential confounders, such as age, sex, substance use, hepatitis C coinfection, cardiovascular risk, and others. …

Hervs And Cancer-A Comprehensive Review Of The Relationship Of Human Endogenous Retroviruses And Human Cancers, Erik Stricker, Erin C Peckham-Gregory, Michael E Scheurer

Center for Medical Ethics and Health Policy Staff Publications

Genomic instability and genetic mutations can lead to exhibition of several cancer hallmarks in affected cells such as sustained proliferative signaling, evasion of growth suppression, activated invasion, deregulation of cellular energetics, and avoidance of immune destruction. Similar biological changes have been observed to be a result of pathogenic viruses and, in some cases, have been linked to virus-induced cancers. Human endogenous retroviruses (HERVs), once external pathogens, now occupy more than 8% of the human genome, representing the merge of genomic and external factors. In this review, we outline all reported effects of HERVs on cancer development and discuss the HERV …

Comparison Of Efficacy And Inflammatory Response To Thermoconjunctivoplasty Performed With Cautery Or Pulsed 1460 Nm Laser, Rodrigo Guimaraes De Souza, David Huang, Scott Prahl, Lauren Nakhleh, Stephen C Pflugfelder

Faculty, Staff and Students Publications

Conjunctivochalasis is a degenerative condition of the conjunctiva that disrupts tear distribution and causes irritation. Thermoreduction of the redundant conjunctiva is required if symptoms are not relieved with medical therapy. Near-infrared laser treatment is a more controlled method to shrink the conjunctiva than thermocautery. This study compared tissue shrinkage, histology, and postoperative inflammation in thermoconjunctivoplasty performed on the mouse conjunctiva using either thermocautery or pulsed 1460 nm near-infrared laser irradiation. Three sets of experiments were performed on female C57BL/6J mice (

Zoledronic Acid Blocks Overactive Kir6.1/Sur2-Dependent Katp Channels In Skeletal Muscle And Osteoblasts In A Murine Model Of Cantú Syndrome, Rosa Scala, Fatima Maqoud, Conor Mcclenaghan, Theresa M Harter, Maria Grazia Perrone, Antonio Scilimati, Colin G Nichols, Domenico Tricarico

2020-Current year OA Pubs

Cantú syndrome (CS) is caused by the gain of function mutations in the