Digital Commons Network^™

Dichloroacetate As A Metabolic Treatment For Glioblastoma, Megan Macia

Yale School of Medicine Physician Associate Program Theses

Glioblastoma is the most common aggressive primary brain tumor in adults, and there is no current standard of care for progression. The Warburg effect is a known part of cancer’s metabolic reprogramming and has been studied as a possible target for limiting tumor growth. Dichloroacetate, a pyruvate dehydrogenase kinase inhibitor, has been proposed as a treatment for glioblastoma, and the drug has had success in phase 1 trials. The proposed study is an open-label, randomized phase 2 clinical trial. Participants will be randomized to oral dichloroacetate, with dosing based on genetic testing, or to a control group treated with lomustine. …

Glycemic Challenge Is Associated With The Rapid Cellular Activation Of The Locus Ceruleus And Nucleus Of Solitary Tract: Circumscribed Spatial Analysis Of Phosphorylated Map Kinase Immunoreactivity, Arshad Khan

Selected Works Temporary Series

Rodent studies indicate that impaired glucose utilization or hypoglycemia is associated with the cellular activation of neurons in the medulla (Winslow, 1733) (MY), believed to control feeding behavior and glucose counterregulation. However, such activation has been tracked primarily within hours of the challenge, rather than sooner, and has been poorly mapped within standardized brain atlases. Here, we report that, within 15 min of receiving 2-deoxy-D-glucose (2-DG; 250 mg/kg, i.v.), which can trigger glucoprivic feeding behavior, marked elevations were observed in the numbers of rhombic brain (His, 1893) (RB) neuronal cell profiles immunoreactive for the cellular activation marker(s), phosphorylated p44/42 MAP …

Disease Risk Of Reintroducing Primates Confiscated From The Illegal Wildlife Trade, Ana Patricia Mendoza Becerra

Dissertations

Zoonotic infections are easily shared between human and other primates, and thus primate trafficking poses a high risk of introducing zoonotic pathogens into urban environments as well as spreading human-associated diseases into natural environments when infected primates are released. The magnitude of these risks has not been thoroughly studied in the context of primate trafficking and rehabilitation of Neotropical primates. In this dissertation, I address some knowledge gaps in disease risk analysis of reintroducing trafficked primates. In chapter 1, I describe the dynamics, diversity, and composition of live wildlife trafficking in urban markets in Peru. I found these markets are …

Nutrient Resorption And Stoichiometric Characteristics Of Wuyi Rock Tea Cultivars, Dehuang Zhu, Suhong Peng, Wenzhen Liu, Shengjie Yu, Dafeng Hui

Biology Faculty Research

Nutrient resorption is an important strategy for plants to retain critical nutrients from senesced leaves and plays important roles in nutrient cycling and ecosystem productivity. As a main economic crop and soil and water conservation species, Wuyi Rock tea has been widely planted in Fujian Province, China. However, foliar nutrient resorptions of Wuyi Rock tea cultivars have not been well quantified. In this study, three Wuyi Rock tea cultivars (Wuyi Jingui, Wuyi Rougui, and Wuyi Shuixian) were selected in the Wuyishan National Soil and Water Conservation, Science and Technology Demonstration Park. Resorption efficiencies of nitrogen (NRE), phosphorus (PRE), and potassium …

Lanza® Tedera Is Strongly Suppressed By Competition From Lolium Multiflorum And Is Best Adapted To Light-Textured Soils, Jesús Fernández-Habas, D. Real, Tom Vanwalleghem, Pilar Fernández-Rebollo

Grain and Other Field Crops Research Articles

Bituminaria bituminosa var. albomarginata, known as Tedera, is a promising forage for Mediterranean climates. An improved variety named LANZA® has been developed. Previous research suggests that soil water saturation in heavy-textured soils might affect its initial development. Competition from grasses could also compromise its successful establishment and persistence. We investigated the effects of soils with contrasting textures (loamy sand vs. clay) with a high soil water content and the competition from Lolium multiflorum on the development of LANZA® in a pot experiment. Dry mass (DM) production of LANZA® was strongly reduced (86%) when grown with L. multiflorum. LANZA® …

Gingival Transcriptomic Patterns Of Macrophage Polarization During Initiation, Progression, And Resolution Of Periodontitis, Octavio A Gonzalez, Sreenatha S Kirakodu, Linh M Nguyen, Jeffrey L Ebersole

Faculty, Staff and Student Publications

Phenotypic and functional heterogeneity of macrophages is clearly a critical component of their effective functions in innate and adaptive immunity. This investigation hypothesized that altered profiles of gene expression in gingival tissues in health, disease, and resolution would reflect changes in macrophage phenotypes occurring in these tissues. The study used a nonhuman primate model to evaluate gene expression profiles as footprints of macrophage variation using a longitudinal experimental model of ligature-induced periodontitis in animals from 3 to 23 years of age to identify aging effects on the gingival environment. Significant differences were observed in distribution of expressed gene levels for …

Toward A Simulation Model Complexity Measure, J. Scott Thompson, Douglas D. Hodson, Michael R. Grimaila, Nicholas Hanlon, Richard Dill

Faculty Publications

Is it possible to develop a meaningful measure for the complexity of a simulation model? Algorithmic information theory provides concepts that have been applied in other areas of research for the practical measurement of object complexity. This article offers an overview of the complexity from a variety of perspectives and provides a body of knowledge with respect to the complexity of simulation models. The key terms model detail, resolution, and scope are defined. An important concept from algorithmic information theory, Kolmogorov complexity, and an application of this concept, normalized compression distance, are used to indicate the possibility of measuring changes …

Glycemic Challenge Is Associated With The Rapid Cellular Activation Of The Locus Ceruleus And Nucleus Of Solitary Tract: Circumscribed Spatial Analysis Of Phosphorylated Map Kinase Immunoreactivity, Khan

Departmental Papers (Biology)

Rodent studies indicate that impaired glucose utilization or hypoglycemia is associated with the cellular activation of neurons in the medulla (Winslow, 1733) (MY), believed to control feeding behavior and glucose counterregulation. However, such activation has been tracked primarily within hours of the challenge, rather than sooner, and has been poorly mapped within standardized brain atlases. Here, we report that, within 15 min of receiving 2-deoxy-D-glucose (2-DG; 250 mg/kg, i.v.), which can trigger glucoprivic feeding behavior, marked elevations were observed in the numbers of rhombic brain (His, 1893) (RB) neuronal cell profiles immunoreactive for the cellular activation marker(s), phosphorylated p44/42 MAP …

Differentiating Between Urban Flood Risk As A Unitary Problem And As A Strand In A Braided Problem Set: Implications For Administrative Coordination, Sarah Michaels

Department of Political Science: Faculty Publications

Flood protection is a leading priority for urban water sustainability. Making cities more resilient to flooding has become urgent as the climate changes and as cities increasingly become the loci of human population and resources. Reducing the risk of future flooding in cities often necessitates different jurisdictions working together. They may do so because they confront a shared problem. This was the case in the City of Lincoln, Nebraska, USA, when partnering agencies shared a single focus on reducing flood risk from Beal Slough to the Nebraska State Penitentiary. In contrast, entities may band together to confront braided problems, intertwined …

Calpain Activity Is Negatively Regulated By A Kctd7-Cullin-3 Complex Via Non-Degradative Ubiquitination, Jaiprakash Sharma, Shalaka Mulherkar, Uan-I Chen, Yan Xiong, Lakshya Bajaj, Byoung-Kyu Cho, Young Ah Goo, Hon-Chiu Eastwood Leung, Kimberley F Tolias, Marco Sardiello

2020-Current year OA Pubs

Calpains are a class of non-lysosomal cysteine proteases that exert their regulatory functions via limited proteolysis of their substrates. Similar to the lysosomal and proteasomal systems, calpain dysregulation is implicated in the pathogenesis of neurodegenerative disease and cancer. Despite intensive efforts placed on the identification of mechanisms that regulate calpains, however, calpain protein modifications that regulate calpain activity are incompletely understood. Here we show that calpains are regulated by KCTD7, a cytosolic protein of previously uncharacterized function whose pathogenic mutations result in epilepsy, progressive ataxia, and severe neurocognitive deterioration. We show that KCTD7 works in complex with Cullin-3 and Rbx1 …

Impact Of Intravenous Fluid Administration On Cardiac Output And Oxygenation During Cardiopulmonary Resuscitation, Jennifer Lutz, Yosef Levenbrown, Md Jobayer Hossain, Anne Hesek, Kelly E. Massa, James P. Keith, Thomas H. Shaffer

Department of Pediatrics Faculty Papers

Background: The efect of intravenous fuid (IVF) administration during cardiopulmonary resuscitation (CPR) is an unexplored factor that may improve cardiac output (CO) during CPR. The aim of this study was to determine the efect of IVF administration on CO and oxygenation during CPR.

Methods: This experimental animal study was performed in a critical care animal laboratory. Twenty-two Landrace-Yorkshire female piglets weighing 27–37 kg were anesthetized, intubated, and placed on positive pressure ventilation. Irreversible cardiac arrest was induced with bupivacaine. CPR was performed with a LUCAS 3 mechanical compression device. Pigs were randomized into IVF or no-IVF groups. Pigs in the …

Convergent Deployment Of Ancestral Functions During The Evolution Of Mammalian Flight Membranes, Charles Y Feigin, Jorge A Moreno, Raul Ramos, Sarah A Mereby, Ares Alivisatos, Wei Wang, Renée Van Amerongen, Jasmin Camacho, John J Rasweiler, Richard R Behringer, Bruce Ostrow, Maksim V Plikus, Ricardo Mallarino

Faculty, Staff and Student Publications

Lateral flight membranes, or patagia, have evolved repeatedly in diverse mammalian lineages. While little is known about patagium development, its recurrent evolution may suggest a shared molecular basis. By combining transcriptomics, developmental experiments, and mouse transgenics, we demonstrate that lateral Wnt5a expression in the marsupial sugar glider (Petaurus breviceps) promotes the differentiation of its patagium primordium. We further show that this function of Wnt5a reprises ancestral roles in skin morphogenesis predating mammalian flight and has been convergently used during patagium evolution in eutherian bats. Moreover, we find that many genes involved in limb development have been redeployed during …

Does Positive Psychology Coaching Improve Trainee Well-Being? Evidence From A Longitudinal Professional Development Coaching Program In A Cohort Of Pediatric Trainees, Benjamin Nelson, Jacqueline T. Chu, Michael G. Healy, Yoon Soo Park, Kerri Palamara Md

Journal of Wellness

Introduction: Trainee burnout is common and evidence supporting the effectiveness of well-being interventions for this population is limited. We studied the effect of a longitudinal coaching program grounded in positive psychology on measures of pediatric trainee well-being.

Methods: Pediatric interns and fellows (n = 67) were enrolled in a positive psychology coaching program in 2017-2019. Pediatric faculty (n = 23) underwent training and were paired with trainees outside their field of interest. Trainees were surveyed at the beginning and end of the program to assess burnout and well-being, and key skills necessary to achieve well-being.

Results: Thirty-one …

Comparing Multiple Sclerosis Age Of Onset In American American And European Cohorts Via Polygenic Risk Scores, Amy Arabel Hernandez

Undergraduate Honors Theses

Health disparities have been observed in autoimmune diseases such as multiple sclerosis (MS), which show that non–Hispanic black patients with MS die at an earlier age and have an increasing mortality trend compared to white MS patients.³ New genetic information shows promising results to treat genetic diseases. For example, genome-wide association studies (GWAS) are being used to calculate polygenic risk scores (PRS) which are a method used to understand disease risk. However, current PRS have been developed using GWAS that overrepresent individuals of European ancestry,⁴ showing that they do not equally apply to all individuals. We set out …

Assessment Of Air Pollution Levels During Sugarcane Stubble Burning Event In La Feria, South Texas, Usa, Sai Deepak Pinakana, Edward Robles, Esmeralda Mendez, Amit U. Raysoni

School of Earth, Environmental, & Marine Sciences Faculty Publications

Agricultural stubble burning is the third largest source of air pollution after vehicular and industrial emissions. Fine particulate matter (PM2.5), volatile organic compounds (VOCs), carbon monoxide (CO), nitrogen dioxide (NO2), and black carbon (BC) are some of the pollutants emitted during such burning events. The Lower Rio Grande Valley (RGV) region of South Texas is a major hub of agricultural activity, and sugarcane farming is one of them. Unfortunately, this activity results in episodic events of high air pollution in this low-resourced, Hispanic/Latino majority region of the U.S.–Mexico border. This study presents results from a sugarcane site in La Feria, …

Coastal Cities In The Southern Us Floodplains: An Evaluation Of Environmental Equity Of Flood Hazards And Social Vulnerabilities, Lubana Tasnim Mazumder

USF Tampa Graduate Theses and Dissertations

Floods are recognized as one of the most common and widespread natural hazards in the United States (US). They are responsible for uncompensated losses and damages. Furthermore, evidence suggests flooding inflicts more harm among socially vulnerable groups, incapable of withstanding hazard events, raising environmental equity (EE) and justice (EJ) concerns. Regrettably, with continuing climate change and urbanization, flooding will not subside in the future; instead, the flooding frequency and intensity will increase in many parts of the US, and the trend of disproportionate impacts will continue to rise.

Since Hurricane Katrina, a considerable amount of research has been conducted on …

Animals Other Than Human Animals And Their Claim To Equal Consideration In Coronavirus And Criminological Study: Examining Harm To Domesticated Dogs During Covid-19 In The Uk, Rebekah Kim Gregory

School of Law, Humanities and Social Sciences

While the financial and social support afforded to United Kingdon (UK) ‘animals other than human animals’ (AOTHAs) welfare charities, such as the RSPCA and Dogs Trust, could suggest that they are valued intrinsically within Western society criminological texts largely omit them from recognition, with some important exceptions, see non-speciesist criminology. Most human animals likely do not want to directly or indirectly harm AOTHAs and even value relationships with “companion” AOTHAs. Regardless, AOTHAs have been victimized throughout history and continue to be. This article examines harm to AOTHAs in the context of the global COVID-19 pandemic to argue that abuse proliferates …

Defects In Lipid Homeostasis Reflect The Function Of Tango2 In Phospholipid And Neutral Lipid Metabolism, Agustin Leonardo Lujan, Ombretta Foresti, Conor Sugden, Nathalie Brouwers, Alex Mateo Farre, Alessio Vignoli, Mahshid Azamian, Alicia Turner, Jose Wojnacki, Vivek Malhotra

Faculty, Staff and Students Publications

We show that TANGO2 in mammalian cells localizes predominantly to mitochondria and partially at mitochondria sites juxtaposed to lipid droplets (LDs) and the endoplasmic reticulum. HepG2 cells and fibroblasts of patients lacking TANGO2 exhibit enlarged LDs. Quantitative lipidomics revealed a marked increase in lysophosphatidic acid (LPA) and a concomitant decrease in its biosynthetic precursor phosphatidic acid (PA). These changes were exacerbated in nutrient-starved cells. Based on our data, we suggest that TANGO2 function is linked to acyl-CoA metabolism, which is necessary for the acylation of LPA to generate PA. The defect in acyl-CoA availability impacts the metabolism of many other …

Calpain Activity Is Negatively Regulated By A Kctd7-Cullin-3 Complex Via Non-Degradative Ubiquitination, Jaiprakash Sharma, Shalaka Mulherkar, Uan-I Chen, Yan Xiong, Lakshya Bajaj, Byoung-Kyu Cho, Young Ah Goo, Hon-Chiu Eastwood Leung, Kimberley F Tolias, Marco Sardiello

Faculty, Staff and Students Publications

Calpains are a class of non-lysosomal cysteine proteases that exert their regulatory functions via limited proteolysis of their substrates. Similar to the lysosomal and proteasomal systems, calpain dysregulation is implicated in the pathogenesis of neurodegenerative disease and cancer. Despite intensive efforts placed on the identification of mechanisms that regulate calpains, however, calpain protein modifications that regulate calpain activity are incompletely understood. Here we show that calpains are regulated by KCTD7, a cytosolic protein of previously uncharacterized function whose pathogenic mutations result in epilepsy, progressive ataxia, and severe neurocognitive deterioration. We show that KCTD7 works in complex with Cullin-3 and Rbx1 …

Med25 Limits Master Regulators That Govern Adipogenesis, Jasmine Saunders, Kunal Sikder, Elizabeth Phillips, Anurag Ishwar, David Mothy, Kenneth B. Margulies, Jason C. Choi

Department of Medicine Faculty Papers

Mediator 25 (Med25) is a member of the mediator complex that relays signals from transcription factors to the RNA polymerase II machinery. Multiple transcription factors, particularly those involved in lipid metabolism, utilize the mediator complex, but how Med25 is involved in this context is unclear. We previously identified Med25 in a translatome screen of adult cardiomyocytes (CMs) in a novel cell type-specific model of LMNA cardiomyopathy. In this study, we show that Med25 upregulation is coincident with myocardial lipid accumulation. To ascertain the role of Med25 in lipid accumulation, we utilized iPSC-derived and neonatal CMs to recapitulate the in vivo …

Role Of The Banyuls(Ban) Gene From Arabidopsis Thaliana In Transgenic Alfalfa Expression Of Anthocyanins And Proanthocyanidins, S. M. Hesamzadeh Hejazi, Sergio Arcioni, F. Paolocci

IGC Proceedings (1977-2023)

Condensed tannins (CTs) are flavonoid oligomers, many of which have beneficial effects on animal (bloat safe) and human health. The BAN gene encodes anthocyanidin reductase (ANR), an enzyme proposed to convert anthocyanidins to their corresponding 2,3-cis-flavan-3-ols (Xie et al., 2003). Ectopic expression of BAN in Alfalfa transgenic foliage results in accumulation of CTs. Thus, it has been assumed that the BAN gene also acts in starter units for the condensation of tannins in Alfalfa.

Diversity Of Diet Composition Decreases With Conjoint Grazing Of Cattle With Sheep And Goats, A. M. Nicol, M. B. Soper, A. Stewart

IGC Proceedings (1977-2023)

Conjoint or mixed grazing can affect the diet selected by each species (Nicol & Collins, 1990). Diet similarity coefficients are often used to compare pairs of diets (Krebs, 1999). However this approach is awkward when a number of contrasts are required in a multifactorial comparison. Species diversity is a descriptor of a particular environment. Many models provide an estimate of species diversity, the most common of these being a log-normal distribution (Tokeshi, 1996). We tested whether this model could be applied to dietary components selected from a pasture, and thus provide a coefficient of dietary diversity for the individual diets …

A Simple Vegetation Criterion (Ndf Content) May Account For Diet Choices Of Cattle Between Forages Varying In Maturity Stage And Physical Accessibility, Cécile Ginane, R. Baumont

IGC Proceedings (1977-2023)

The management of extensively grazed pastures requires an understanding and prediction of the diet choices of herbivores grazing on vegetation that is qualitatively (maturity stage) and quantitatively (biomass, sward height) heterogeneous. The Optimal Foraging Theory (OFT, Stephens & Krebs, 1986), bases its predictions on the relative energy intake rate (EIR) of forages. However, as EIRs are difficult to assess at pasture and are subject to wide intra- and inter-individual variations, another vegetation criterion was sought (accessibility, quality), by-passing the animal's influence, to predict cattle diet choices quantitatively.

Spatial Scale Of Heterogeneity Affects Diet Choice But Not Intake In Beef Cattle, S. Mark Rutter, J. E. Cook, K. L. Young, R. A. Champion

IGC Proceedings (1977-2023)

Previous research has shown that sheep (Champion et al., 1998) and dairy cattle (Nuthall et al., 2000) have a partial preference for clover of 70%, and achieve higher daily intakes when offered grass and clover as separate but adjacent monocultures compared with animals grazing mixed swards. This intake benefit could be utilised to increase intake and production on farms by grazing from adjacent strips of the two herbages. This study aimed to establish the minimum strip width required to achieve the benefits of monocultures.

Herders And Wetland Degradation In Northern Cameroon, E. Tedonkeng Pamo, F. Tendonkeng, J. R. Kana

IGC Proceedings (1977-2023)

Livestock rearing in Northern Cameroon is carried out under two majors systems: the nomadic and the transhumance production systems (Pamo & Pamo, 1991). Nomadism is the practice of wandering from place to place, while transhumance involves seasonal displacement of flocks from one area to another by herders. These production systems involved large grazing areas, which may encompass different ecosystems. The Yaére, the only wetland of the northern Cameroon, is the major dry season grazing lands for livestock and wildlife. The main characteristic of this wetland is that the whole area is excluded from grazing during the growing season as a …

To Be Necessary: The Remarkable Life Of Mary Wollstonecraft, Elisabeth Phillips

Bound Away: The Liberty Journal of History

Although overshadowed by her daughter, Mary Shelley, in the public imagination, Mary Wollstonecraft (1759-1797) stands as a significant figure in her time who left a significant legacy. Her writings advocating for women’s education, equal rights, and career opportunities established her as the progenitor of the modern women’s rights movement. Wollstonecraft’s ideas resonated in the era of the Atlantic world revolutions and laid the foundation for later advances of women in the Western world; therefore, it is important to study her contributions in the present.

The Chanticleer, 2023-03-23, Coastal Carolina University

The Chanticleer Student Newspaper

The editorially independent student produced weekly newspaper of Coastal Carolina University.

Issue 414: March 23-April 6, 2023, The Local Voice

The Local Voice

No abstract provided.

Fall 2022 Student Textbook Survey Results, Krystie Wilfong, Janelle Wertzberger, Mary Elmquist, Laura A. Conforte

ILS Scholarship

During the Fall 2022 semester, eleven Oberlin Group institutions in association with the Open and Equitable Access to Scholarship Working Group conducted a student textbook survey adapted from the 2016 Florida Virtual Campus survey and the 2019 Gettysburg College survey. The goal of the project was to gain a better understanding of how the costs of course materials impact our own students. This presentation provides an overview of those results as well as action steps libraries can take to better support our students.

This study was coordinated by the Oberlin Group - Open and Equitable Working Group.

March 23, 2023, The Daily Mississippian

Daily Mississippian (all digitized issues)

No abstract provided.