Digital Commons Network^™

Southern Sea Otter (Enhydra Lutris Nereis) Fur Morphology, Thermal Function, And Buoyancy Across Ontogeny, Kate Riordan

Master's Theses

Sea otters (Enhydra lutris) have the densest fur of any animal, and the hairs function to trap a layer of air that is used for insulation. When a sea otter is born, it has a natal pelage (baby fur), and sea otters eventually molt that fur and replace it with a pelt resembling the adult fur. Sea otter fur is composed of 3 types of hairs: underhairs, intermediate hairs, and guard hairs. Sea otters are more susceptible to the negative effects of oiling from oil spills compared to other marine mammal species because they solely rely on fur …

Aryl Hydrocarbon Receptor Maintains Hepatic Mitochondrial Homeostasis In Mice, Mi Jeong Heo, Ji Ho Suh, Sung Ho Lee, Kyle L Poulsen, Yu A An, Bhagavatula Moorthy, Sean M Hartig, David D Moore, Kang Ho Kim

Faculty, Staff and Student Publications

OBJECTIVE: Mitophagy removes damaged mitochondria to maintain cellular homeostasis. Aryl hydrocarbon receptor (AhR) expression in the liver plays a crucial role in supporting normal liver functions, but its impact on mitochondrial function is unclear. Here, we identified a new role of AhR in the regulation of mitophagy to control hepatic energy homeostasis.

METHODS: In this study, we utilized primary hepatocytes from AhR knockout (KO) mice and AhR knockdown AML12 hepatocytes. An endogenous AhR ligand, kynurenine (Kyn), was used to activate AhR in AML12 hepatocytes. Mitochondrial function and mitophagy process were comprehensively assessed by MitoSOX and mt-Keima fluorescence imaging, Seahorse XF-based …

A 3d-Printable, Low-Cost Obturator For Less Invasive Gynecologic Brachytherapy, Sanika Rane, Alexander Hanania, Elisa Arango, Krithika Kumar, Lauren Payne, Susannah Dittmar, Gaurav Gomber, Vincent Ugarte, Michelle Ludwig

Faculty, Staff and Students Publications

The purpose of this report is to design, develop, and evaluate a cost-effective applicator for interstitial brachytherapy (ISBT) to minimize patient morbidity and facilitate access to curative radiation treatment for gynecologic cancers, especially in low-resource settings. A computer-aided design and prototype were developed of a proposed applicator that incorporates 44 slotted channels to gently guide needles, with or without a tandem, through the vaginal canal, effectively eliminating the need for transcutaneous needle insertions typically employed during ISBT of advanced gynecologic cancer and thus reducing the risk of vaginal laceration and bladder or rectal injury. The tested prototype was developed using …

Attainable And Sustainable Home Building Solutions, Katrina Matthews

Construction Management

Sustainability has fortunately been a recent concern in the construction industry as climate change continues to impose potentially insurmountable threats to the global environment. Although many new methods have been adopted in efforts to lessen the adverse environmental effects of construction-related activities, as a society, we still have much room for growth as the built environment accounts for almost 50% of global emissions. Many changes and standards must be adopted to make these solutions efficiently beneficial. To set industry standards, we must start with the most basic form of construction- residential building. This report covers two attainable framing solutions for …

French Drain Project At Woods Humane Society, Thomas Douglas Collins

Construction Management

The Woods Humane Society is a prominent animal care facility that aims to rehabilitate, rescue, and assist in the adoption of dogs and cats in the greater San Luis Obispo area. The property contains multiple dog playpen areas which consist of a concrete slab surrounded by a chain link fence, designed for the animals to have an open space to run around throughout the day. Employees are required to hose down the slab hourly to maintain a clean environment for the animals. When employees hose down the playpen areas, a considerable amount of water runs off the slab and into …

French Drain Construction For Woods Humane Society, Cade M. Rees

Construction Management

The Woods Humane Society is a prominent animal care facility that aims to rehabilitate, rescue, and assist in the adoption of dogs and cats in the greater San Luis Obispo area. The property contains multiple dog playpen areas which consist of a concrete slab surrounded by a chain link fence, designed for the animals to have an open space to run around throughout the day. Employees are required to hose down the slab hourly to maintain a clean environment for the animals. When employees hose down the playpen areas, a considerable amount of water runs off the slab and into …

Deficiency In Galectin-3, -8, And -9 Impairs Immunity To Chronic Mycobacterium Tuberculosis Infection But Not Acute Infection With Multiple Intracellular Pathogens, Huntly M. Morrison, Wandy L. Beatty, Et Al.

2020-Current year OA Pubs

Macrophages employ an array of pattern recognition receptors to detect and eliminate intracellular pathogens that access the cytosol. The cytosolic carbohydrate sensors Galectin-3, -8, and -9 (Gal-3, Gal-8, and Gal-9) recognize damaged pathogen-containing phagosomes, and Gal-3 and Gal-8 are reported to restrict bacterial growth via autophagy in cultured cells. However, the contribution of these galectins to host resistance during bacterial infection in vivo remains unclear. We found that Gal-9 binds directly to Mycobacterium tuberculosis (Mtb) and Salmonella enterica serovar Typhimurium (Stm) and localizes to Mtb in macrophages. To determine the combined contribution of membrane damage-sensing galectins to immunity, we generated …

Temperament & Character Account For Brain Functional Connectivity At Rest: A Diathesis-Stress Model Of Functional Dysregulation In Psychosis, Igor Zwir, Javier Arnedo, Alberto Mesa, Coral Del Val, Gabriel A De Erausquin, C Robert Cloninger

2020-Current year OA Pubs

The human brain's resting-state functional connectivity (rsFC) provides stable trait-like measures of differences in the perceptual, cognitive, emotional, and social functioning of individuals. The rsFC of the prefrontal cortex is hypothesized to mediate a person's rational self-government, as is also measured by personality, so we tested whether its connectivity networks account for vulnerability to psychosis and related personality configurations. Young adults were recruited as outpatients or controls from the same communities around psychiatric clinics. Healthy controls (n = 30) and clinically stable outpatients with bipolar disorder (n = 35) or schizophrenia (n = 27) were diagnosed by structured interviews, and …

The Voltage-Gated Sodium Channel In Drosophila, Para, Localizes To Dendrites As Well As Axons In Mechanosensitive Chordotonal Neurons, Thomas A Ravenscroft, Ashleigh Jacobs, Mingxue Gu, Daniel F Eberl, Hugo J Bellen

Faculty, Staff and Students Publications

The fruit fly Drosophila melanogaster has provided important insights into how sensory information is transduced by transient receptor potential (TRP) channels in the peripheral nervous system (PNS). However, TRP channels alone have not been able to completely model mechanosensitive transduction in mechanoreceptive chordotonal neurons (CNs). Here, we show that, in addition to TRP channels, the sole voltage-gated sodium channel (NaV) in Drosophila, Para, is localized to the dendrites of CNs. Para is localized to the distal tip of the dendrites in all CNs, from embryos to adults, and is colocalized with the mechanosensitive TRP channels No mechanoreceptor potential C …

The Role Of Limch1 Alternative Splicing In Skeletal Muscle Function, Matthew S Penna, Rong-Chi Hu, George G Rodney, Thomas A Cooper

Faculty, Staff and Students Publications

Postnatal skeletal muscle development is a highly dynamic period associated with widespread alternative splicing changes required to adapt tissues to adult function. These splicing events have significant implications because the reversion of adult mRNA isoforms to fetal isoforms is observed in forms of muscular dystrophy. LIMCH1 is a stress fiber–associated protein that is alternatively spliced to generate uLIMCH1, a ubiquitously expressed isoform, and mLIMCH1, a skeletal muscle–specific isoform containing six additional exons simultaneously included after birth in the mouse. CRISPR/Cas9 was used to delete the six alternatively spliced exons of LIMCH1 in mice, thereby forcing the constitutive expression of the …

Pediatric Swine Model Of Methicillin-Resistant Staphylococcus Aureus Sepsis-Induced Coagulopathy, Disseminated Microvascular Thrombosis, And Organ Injuries, Trung C Nguyen, Juan C Marini, Bobby Guillory, Christian Valladolid-Brown, Marina Martinez-Vargas, Deepika Subramanyam, Daniel Cohen, Sonya C Cirlos, Fong Lam, Barbara Stoll, Inka C Didelija, Caitlin Vonderohe, Renan Orellana, Arun Saini, Subhashree Pradhan, Dalia Bashir, Moreshwar S Desai, Saul Flores, Manpreet Virk, Hossein Tcharmtchi, Amir Navaei, Sheldon Kaplan, Linda Lamberth, Kristina G Hulten, Brooks P Scull, Carl E Allen, Ayse Akcan-Arikan, K Vinod Vijayan, Miguel A Cruz

Faculty, Staff and Students Publications

Sepsis-induced coagulopathy leading to disseminated microvascular thrombosis is associated with high mortality and has no existing therapy. Despite the high prevalence of Gram-positive bacterial sepsis, especially methicillin-resistant

HYPOTHESIS: Our objective is to develop a pediatric sepsis-induced coagulopathy swine model that last 70 hours.

METHODS AND MODELS: Ten 3 weeks old piglets, implanted with telemetry devices for continuous hemodynamic monitoring, were IV injected with MRSA (

RESULTS: Compared with the saline treated piglets (control), the septic piglets within 24 hours had significantly lower neurologic and respiratory scores. Over time, PT, d-dimer, and fibrinogen increased, while platelet counts and activities of factors …

Bi-Allelic Variants In Hmgcr Cause An Autosomal-Recessive Progressive Limb-Girdle Muscular Dystrophy, Joel A Morales-Rosado, Tanya L Schwab, Sarah K Macklin-Mantia, A Reghan Foley, Filippo Pinto E Vairo, Davut Pehlivan, Sandra Donkervoort, Jill A Rosenfeld, Grace E Boyum, Ying Hu, Anh T Q Cong, Timothy E Lotze, Carrie A Mohila, Dimah Saade, Diana Bharucha-Goebel, Katherine R Chao, Christopher Grunseich, Christine C Bruels, Hannah R Littel, Elicia A Estrella, Lynn Pais, Peter B Kang, Michael T Zimmermann, James R Lupski, Brendan Lee, Matthew J Schellenberg, Karl J Clark, Klaas J Wierenga, Carsten G Bönnemann, Eric W Klee

Faculty, Staff and Students Publications

Statins are a mainstay intervention for cardiovascular disease prevention, yet their use can cause rare severe myopathy. HMG-CoA reductase, an essential enzyme in the mevalonate pathway, is the target of statins. We identified nine individuals from five unrelated families with unexplained limb-girdle like muscular dystrophy and bi-allelic variants in HMGCR via clinical and research exome sequencing. The clinical features resembled other genetic causes of muscular dystrophy with incidental high CPK levels (>1,000 U/L), proximal muscle weakness, variable age of onset, and progression leading to impaired ambulation. Muscle biopsies in most affected individuals showed non-specific dystrophic changes with non-diagnostic immunohistochemistry. …

June 2023 News, Office Of Sustainability

Office of Sustainability Newsletter

No abstract provided.

The End Of Balancing? Text, History & Tradition In First Amendment Speech Cases After Bruen, Clay Calvert, Mary-Rose Papandrea

Duke Journal of Constitutional Law & Public Policy

This Article examines the potential impact on First Amendment free-speech jurisprudence of the U.S. Supreme Court's increasing reliance on text, history, and tradition in 2022 decisions such as New York State Rifle & Pistol Association v. Bruen. In Bruen, the Court embraced a new test for examining Second Amendment cases. It concentrates on whether there is a historical tradition of regulating the conduct in question, and it eliminates any use of constitutionally common means-end standards of review such as strict and intermediate scrutiny. Those two scrutiny standards often guide the Court's free-speech decisions. The Bruen majority, however, asserted …

Fat Stories, Susan Stinson

English Language and Literature: Faculty Books

The Contemporary Reader of Gender and Fat Studies is a key reference work in contemporary scholarship situated at the intersection between Gender and Fat Studies, charting the connections and tensions between these two fields.

Comprising over 20 chapters from a range of diverse and international contributors, the Reader is structured around the following key themes: theorizing gender and fat; narrating gender and fat; historicizing gender and fat; institutions and public policy; health and medicine; popular culture and media; and resistance. It is an intersectional collection, highlighting the ways that "gender" and "fat" always exist in connection with multiple other structures, …

A Case For Accelerating Standards To Achieve The Fair Principles Of Environmental Health Research Experimental Data, Rance Nault, Matthew C. Cave, Gabriele Ludewig, Hunter N. B. Moseley, Kelly G. Pennell, Tim Zacharewski

Markey Cancer Center Faculty Publications

BACKGROUND: Funding agencies, publishers, and other stakeholders are pushing environmental health science investigators to improve data sharing; to promote the findable, accessible, interoperable, and reusable (FAIR) principles; and to increase the rigor and reproducibility of the data collected. Accomplishing these goals will require significant cultural shifts surrounding data management and strategies to develop robust and reliable resources that bridge the technical challenges and gaps in expertise.

OBJECTIVE : In this commentary, we examine the current state of managing data and metadata—referred to collectively as (meta)data—in the experimental environmental health sciences. We introduce new tools and resources based on in vivo …

Novel Isolation Method Reveals Sex-Specific Composition And Neurotoxicity Of Small Extracellular Vesicles In A Mouse Model Of Alzheimer’S Disease, Ahmed Elsherbini, Zhihui Zhu, Zainuddin Quadri, Simone M. Crivelli, Xiaojia Ren, Hemendra J. Vekaria, Priyanka Tripathi, Liping Zhang, Wenbo Zhi, Erhard Bieberich

Markey Cancer Center Faculty Publications

We developed a new method to isolate small extracellular vesicles (sEVs) from male and female wild-type and 5xFAD mouse brains to investigate the sex-specific functions of sEVs in Alzheimer’s disease (AD). A mass spectrometric analysis revealed that sEVs contained proteins critical for EV formation and Aβ. ExoView analysis showed that female mice contained more GFAP and Aβ-labeled sEVs, suggesting that a larger proportion of sEVs from the female brain is derived from astrocytes and/or more likely to bind to Aβ. Moreover, sEVs from female brains had more acid sphingomyelinase (ASM) and ceramide, an enzyme and its sphingolipid product important for …

The Kappa Opioid Receptor Agonist 16-Bromo Salvinorin A Has Anti-Cocaine Effects Without Significant Effects On Locomotion, Food Reward, Learning And Memory, Or Anxiety And Depressive-Like Behaviors, Ross Van De Wetering, Amy Ewald, Susan Welsh, Lindsay Kornberger, Samuel E. Williamson, Bryan D. Mcelroy, Eduardo R. Butelman, Thomas E. Prisinzano, Bronwyn M. Kivell

Markey Cancer Center Faculty Publications

Kappa opioid receptor (KOR) agonists have preclinical antipsychostimulant effects; however, adverse side effects have limited their therapeutic development. In this preclinical study, conducted in Sprague Dawley rats, B6-SJL mice, and non-human primates (NHPs), we evaluated the G-protein-biased analogue of salvinorin A (SalA), 16-bromo salvinorin A (16-BrSalA), for its anticocaine effects, side effects, and activation of cellular signaling pathways. 16-BrSalA dose-dependently decreased the cocaine-primed reinstatement of drug-seeking behavior in a KOR-dependent manner. It also decreased cocaine-induced hyperactivity, but had no effect on responding for cocaine on a progressive ratio schedule. Compared to SalA, 16-BrSalA had an improved side effect profile, with …

Development Of Novel, Brain Permeable, Mpges-1 Inhibitors For Inflammatory Conditions, Madison Sluter

Theses and Dissertations (ETD)

Prostaglandin E2 (PGE2) is a potent proinflammatory lipid signaling molecule produced downstream of the cyclooxygenase (COX) cascade. The COX pathway has long been implicated for its role in inflammation, with drugs like aspirin and other non-steroidal anti-inflammatory drugs (NSAIDs) non-selectively targeting the upstream COX enzymes to suppress inflammation. However, despite displaying potent anti-inflammatory effects it has been demonstrated that when taken chronically, NSAIDs can cause gastrointestinal bleeding. COX-2 selective inhibitors (COXIBs) were then developed to combat the gastrointestinal bleeding induced by nonselective NSAIDs by specifically inhibiting the COX enzyme most infamous for its role in inflammation. Yet, soon after entering …

The Interaction Between Host G3bp And Viral Nucleocapsid Protein Regulates Sars-Cov-2 Replication, Zemin Yang

Theses and Dissertations (ETD)

G3BP1/2 are RNA-binding proteins that promote condensation to form stress granules in response to various cellular stresses, including viral infection. G3BP1/2 are prominent interactors of the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the functional consequences of the G3BP-N interaction in the context of viral infection have remained unclear. Here we used structural and biochemical analyses to define the residues required for G3BP1-N interaction, followed by structure-guided mutagenesis of G3BP1 and N to selectively disrupt their interaction. We found that mutation of N-F17 led to selective loss of interaction with G3BP1 and consequent failure of …

Natural Resource Management Newsletter, June 2023, Department Of Natural Resource Management

NRM Newsletter

Page 1: Departmental Faculty and Staff, and Dean's Message
Page 2: NRM Newbies
Page 3: Emeriti Publications and Award, Donor Obituary, Recent Graduate News, and Ten Year Service Award
Page 4: Donor News
Page 5: Sampling of Faculty and Staff Activities & Recognition
Page 6: Sampling of Graduate Students Activities
Page 7: Sampling of Undergraduate Students Activities
Page 8: Club Activities
Page 9: NRM Events
Page 10: Opportunities to Support NRM

Potential Effect Of Different Pulp Capping Materials On Human Dental Pulp Stem Cells: A Laboratory Study, Mohamed A. Bedir, Laila Amin, Essam Soussa

Mansoura Journal of Dentistry

Objective: This study aimed to evaluate the regenerative capacity of two different pulp capping materials TheraCal LC and Nano hydroxyapatite on stem cells extracted from the human dental pulp as a treatment modality for pulp capping. Materials and Methods: Human dental pulp stem cells were obtained and characterized. They were prepared in 12-well plates, cultured in standard culture media, and separated into three groups with 12 each. The first group served as the negative control group and contained only human dental pulp stem cells; the second group contained TheraCal LC, which was incubated with human dental pulp stem cells; and …

Do Cover Crop Mixtures Improve Soil Physical Health More Than Monocultures?, Humberto Blanco-Canqui

Department of Agronomy and Horticulture: Faculty Publications

Rationale and Purpose — Adding multispecies cover crop (CC) mixtures could diversify the current simplified crop rotations and enhance soil health more than monoculture CCs. Further, CC mixtures with diverse plant species could adapt better to changing climatic and environmental conditions than monoculture CCs. However, our current understanding of the soil benefits of CC mixtures is still limited. This review discussed whether CC mixtures are better than monoculture CCs to improve soil physical health.

Methods — All studies published up to May 25, 2023, comparing soil physical properties between CC mixtures and their constituents grown as monocultures were searched in …

Plestiodon Egregius Insularis (Cedar Key Mole Skink). Reproduction., Jake Scott, Kevin M. Enge, Louis A. Somma, Richard D. Bartlett

Papers in Herpetology

PLESTIODON EGREGIUS INSULARlS (Cedar Key Mole Skink). REPRODUCTION. Plestiodon egregius insularis is the largest of five described subspecies (Mount 1965. Bull. Florida St. Mus. BioI. Sci. 9:183-213) and is known from only nine small islands off the coast of Levy County, Florida, USA. The only documented clutch size for P. e. insularis contained five eggs (Mount 1963. Am. MidI. Nat. 70:356-385). For wild P. egregius, Mount (1963, op. cit.) reported 2-9 (mean = 4.8) eggs for 13 clutches, and Hamilton and Pollack (1958. Herpetologica 14:25-28) found two nests of P. e. similis in Georgia containing five eggs each. In captivity, …

Using The ‘4ps’ Social Marketing Strategy To Overcome Vaccination Hesitancy: Covid-19 Vaccine Coverage In A Chinese College As An Example, Baojia Zheng, Jiao Yan, Xiaoqing Wang, Yuxin Tang, Yali Xiang

Saudi Medical Journal

Objectives: To explore the role of the ‘4Ps’ social marketing strategy on vaccine hesitancy and its impact on public vaccine coverage. The ‘4Ps’ strategy (place, price, product and promotion) is a social marketing tool that had addressed many public issues and vaccinations. Methods: This study was a retrospective before-after study. A total of 10795 people were enrolled. The routine COVID-19 immunization method was used for the first vaccination doses from 23 March to 11 April, 2021. Then we carried out a ‘4Ps’ social marketing strategy for vaccination from 12 April to 1 May 2021. The vaccine hesitancy and the coverage …

Assessment Of Sharps Penetrating Injury, Mucosal Exposure And Compliance With Standard Precautions Of Health Workers At A University Hospital In Turkey, Hıdır Sarı, Saim Dayan, Hüsna Balkan, Yeliz Çiçek, Mehmet Özel

Saudi Medical Journal

Objectives: To evaluate the compliance with standard precautions in healthcare workers who experienced a sharps penetrating injury or mucosal exposure using the compliance with Standard Precautions Scale, Turkish version. Methods: This descriptive cross-sectional study was conducted in a tertiary hospital. The occupational health and safety unit records of healthcare workers who were injured by a sharps penetrating injury or experienced mucosal exposure between January 2018 and July 2020 were examined. Compliance with the Standard Precautions Scale was assessed by having participants answer a questionnaire. Results: Of the 100 participants, 59% were men. Of all healthcare workers in the university hospital, …

Slab Extension At Woods Humane Society, Corey K. Smith

Construction Management

Woods Humane Society is an animal shelter that provides pet rehabilitation and adoption services to San Luis Obispo and surrounding areas. The Woods’ facility contains a number of kennels for dogs as well as numerous outdoor, fenced-in, concrete exercise yards. These yards give dogs the opportunity to play outside a few times a day allowing them to get vital exercise. One of these yards on the south side of the property had an exterior fence that was offset from an existing slab on the interior of the fence by one foot. This was an issue for the shelter as dogs …

Estimation Of Quantitative Genetic Parameters Of The Eastern Oyster (Crassostrea Virginica) In The Gulf Of Mexico, Heather King

Master's Theses

The decline of natural oyster reefs in Gulf of Mexico (Gulf) states has stimulated the development of aquaculture to support the industry and restoration programs. Gulf habitats feature diverse salinity environments that may require oysters bred for habitat-specific optimal genetic characteristics. In this work, genetic parameters for performance in habitats with different salinity were estimated. In September 2020, 102 males and 102 females sourced from 17 natural reefs across the northern Gulf were bred according to 2 males x 2 females non-overlapping factorial crossing designs, resulting in 204 full and half sibs. All families were pooled after fertilization according to …

City Farm Slo Fencing Upgrade, Jorge Ignacio Hernandez

Construction Management

This paper outlines the process of a fence upgrade that was built for a non-profit organization in San Luis Obispo, CA called City Farm SLO. The City Farm SLO is a non-profit organization that educates locals how to grow healthy and sustainable foods. This new fencing project spans a total of 422 feet with three new farm gates. The process that was approached for this project was to have it planned in three different phases. The first phase was the pre-construction phase, which consisted of meetings with the owner, creating a design, making a materials list, estimating the total cost …

3d Printed Poly Lactic Acid Structures For Cell Growth And Nanoparticle Transport-Simulation And Experimental Validation, Shrikrushna Vinayak Sonawane

Masters Theses

In today's world, 3D printing or additive manufacturing (AM) is being widely used in various fields due to its benefits such as rapid prototyping, reduced material waste and energy usage, lower cost, and more. Even the tissue engineering field is utilizing this technology. This study focused on examining the compatibility of 3D printed Lab-on-a-Chip structures made of Poly Lactic Acid (PLA) for in-vitro applications. In this research, the Lab-on-a-Chip structures were designed and created using 3D printing. To achieve the required bio-functionality scaffold surface was customized by post-printing processes such as mechanical polishing, hydrolysis (Wet Chemical Etching), and ultraviolet light …