Digital Commons Network^™

High Dynamic Range 3d Motion Tracking Using Circular Scans With Optical Coherence Tomography, Senyue Hao, Marcello Magri Amaral, Chao Zhou

2020-Current year OA Pubs

Motion artifacts, from such sources as heartbeats, respiration, or peristalsis, often degrade microscopic images or videos of live subjects. We have developed a method using circular optical coherence tomography (OCT) scans to track the transverse and axial motion of biological samples at speeds ranging from several micrometers per second to several centimeters per second. We achieve fast and high-precision measurements of the magnitude and direction of the sample's motion by adaptively controlling the circular scan pattern settings and applying interframe and intraframe analyses. These measurements are the basis of active motion compensation via feedback control for future

Time-Dependent Water Wave Scattering By A Marine Structure Consisting Of An Array Of Compound Porous Cylinders, Sn Bora, S Das, Mh Meylan, S Saha, S Zheng

School of Engineering, Computing and Mathematics

Under the assumption of linear wave theory, a semi-analytical model is developed to address the time-dependent water wave scattering problem involving a marine structure consisting of several circular rigid vertical cylinders, each of which is surrounded by a thin cylindrical porous wall with a water region between the inner cylinder and the wall. The problem is tackled by applying the eigenfunction expansion approach. The energy dissipation relation is also derived for the system of compound cylinders. The principal focus of this work lies in locating the optimum geometrical configurations for which the wave forces acting on the structure are minimal …

Anti-Scg3 Gene Therapy To Treat Choroidal Neovascularization In Mice, Chengchi Huang, Liyang Ji, Avinash Kaur, Hong Tian, Prabuddha Waduge, Keith A Webster, Wei Li

Faculty, Staff and Students Publications

Neovascular age-related macular degeneration (nAMD) with choroidal neovascularization (CNV) is a leading cause of blindness in the elderly in developed countries. The disease is currently treated with anti-angiogenic biologics, including aflibercept, against vascular endothelial growth factor (VEGF) but with limited efficacy, treatment resistance and requirement for frequent intravitreal injections. Although anti-VEGF gene therapy may provide sustained therapy that obviates multiple injections, the efficacy and side effects related to VEGF pathway targeting remain, and alternative strategies to block angiogenesis independently of VEGF are needed. We recently reported that secretogranin III (Scg3) induces only pathological angiogenesis through VEGF-independent pathways, and Scg3-neutralizing antibodies …

Adjuvanted Fusion Protein Vaccine Induces Durable Immunity To Onchocerca Volvulus In Mice And Non-Human Primates, Nathan M Ryan, Jessica A Hess, Erica J Robertson, Nancy Tricoche, Cheri Turner, Jenn Davis, Nikolai Petrovsky, Melissa Ferguson, William J Rinaldi, Valerie M Wong, Ayako Shimada, Bin Zhan, Maria Elena Bottazzi, Benjamin L Makepeace, Sean A Gray, Darrick Carter, Sara Lustigman, David Abraham

Faculty, Staff and Students Publications

Onchocerciasis remains a debilitating neglected tropical disease. Due to the many challenges of current control methods, an effective vaccine against the causative agent Onchocerca volvulus is urgently needed. Mice and cynomolgus macaque non-human primates (NHPs) were immunized with a vaccine consisting of a fusion of two O. volvulus protein antigens, Ov-103 and Ov-RAL-2 (Ov-FUS-1), and three different adjuvants: Advax-CpG, alum, and AlT4. All vaccine formulations induced high antigen-specific IgG titers in both mice and NHPs. Challenging mice with O. volvulus L3 contained within subcutaneous diffusion chambers demonstrated that Ov-FUS-1/Advax-CpG-immunized animals developed protective immunity, durable for …

Book Review: Just Schools: Building Equitable Collaborations With Families And Communities, Tara Bartlett

Journal of Educational Research and Practice

K–12 schools are facing a syndemic of critical junctures right now: On the one hand, school communities and families are still reeling from the effects of racial reckonings and the COVID-19 pandemic, while on the other hand, school leaders and decision-makers are grappling with how to effectively engage families and students amid demands and realizations for equity and justice. The book Just Schools: Building Equitable Collaborations with Families and Communities (2020) by Ann Ishimaru provides tools and pathways forward. Ishimaru first describes how our education systems have long been incubators of oppression and disengagement, steeped in colonizing methods and deficit …

The Role Of Using Images In Outdoor Advertising Designs To Attract Attention, Sattar Al-Juboori

Middle East Journal of Communication Studies

Outdoor advertising has dominated the visual landscape of cities around the world. Outdoor advertising in city streets and public places are different than the ones located in other places for many reasons. First, it is directed towards particular audience who has no option but to look at it. The research problem lies in the excessive use of images in ways that may have been randomly put without taking into consideration certain factors such as allocation, processing, design construction or some expressive and psychological aspects. Experts have defined some technical problems related to image-choice and the extent of its future impact …

Adsorptive–Photocatalytic Performance For Antibiotic And Personal Care Product Using Cu0.5Mn0.5Fe2O4, Chanat Chokejaroenrat, Chainarong Sakulthaew, Athaphon Angkaew, Apiladda Pattanateeradetch, Wuttinun Raksajit, Kanokwan Teingtham, Piyaporn Phansak, Pawee Klongvessa, Daniel D. Snow, Clifford E. Harris, Steven Comfort

Nebraska Water Center: Faculty Publications

The amount of antibiotics and personal care products entering local sewage systems and ultimately natural waters is increasing and raising concerns about long-term human health effects. We developed an adsorptive photocatalyst, Cu_0.5Mn_0.5Fe₂O₄ nanoparticles, utilizing co-precipitation and calcination with melamine, and quantified its efficacy in removing paraben and oxytetracycline (OTC). During melamine calcination, Cu_0.5Mn_0.5Fe₂O₄ recrystallized, improving material crystallinity and purity for the adsorptive–photocatalytic reaction. Kinetic experiments showed that all four parabens and OTC were removed within 120 and 45 min. We found that contaminant adsorption and reaction …

Minutes 7-5-2023 | Institutional Animal Care & Use Committee, Annette Carter

Institutional Animal Care & Use Minutes

No abstract provided.

Yes-Associated Protein-1 Overexpression In Ocular Surface Squamous Neoplasia; A Potential Diagnostic Marker And Therapeutic Target, Peter Julius, Stepfanie N. Siyumbwa, Fred Maate, Phyllis Moonga, Guobin Kang, Trevor Kaile, John T. T., Charles Wood

Nebraska Center for Virology: Faculty Publications

Yes-associated protein-1 (YAP-1) is a Hippo system transcription factor, which serves as an oncogene in squamous cell carcinoma, and several solid tumors when the Hippo pathway is dysregulated. Yet, the activity of YAP-1 in ocular surface squamous neoplasia (OSSN) has not been determined. Here, we investigate the relationship between YAP-1 overexpression and OSSN. Using a cross-sectional study design, we recruited 227 OSSN patients from the University Teaching Hospitals in Lusaka, Zambia. Immunohistochemistry was used to assess YAP-1 protein overexpression in tumor tissue relative to surrounding benign squamous epithelium. OSSN patient samples (preinvasive, n = 62, 27% and invasive, n = …

Genotype By Environment Interaction And Heteroscedasticity Influence The Expression Of Parasite Resistance In Katahdin Sheep, Brian C. Arisman, Joan M. Burke, James L. M. Morgan, Ronald M. Lewis

Department of Animal Science: Faculty Publications

Increasingly, sheep producers are choosing breeds that express resistance to gastrointestinal parasites due to reduced efficacy of anthelminthic drugs. One such breed is Katahdin. Katahdins are raised in various climates and management systems in the United States, which can be combined into eco-management clusters to describe production environments more holistically. The objectives of this study were to determine if genotype by environment interaction (G × E) and heteroscedasticity existed across these eco-management clusters for traits indicative of parasite resistance. Body weights (BW), FAMACHA scores (FAM), and fecal egg counts (FEC) were collected at around 90 d in 3,527 Katahdin lambs …

Antimicrobial Resistance: A Growing Serious Threat For Global Public Health, Md Abdus Salam, Md Yusuf Al-Amin, Moushumi Tabassoom Salam, Jogendra Singh Pawar, Naseem Akhter, Ali A. Rabaan, Mohammed A. A Alqumber

Neurology Articles

Antibiotics are among the most important discoveries of the 20th century, having saved millions of lives from infectious diseases. Microbes have developed acquired antimicrobial resistance (AMR) to many drugs due to high selection pressure from increasing use and misuse of antibiotics over the years. The transmission and acquisition of AMR occur primarily via a human-human interface both within and outside of healthcare facilities. A huge number of interdependent factors related to healthcare and agriculture govern the development of AMR through various drug-resistance mechanisms. The emergence and spread of AMR from the unrestricted use of antimicrobials in livestock feed has been …

Interpreting “Knowingly” To Establish Criminal Liability In Environmental Law: A Modified Public Welfare Approach, Jesse Harte Edelman

Loyola of Los Angeles Law Review

Now, more than ever, environmental disasters occur on an unprecedented scale. The main objective of environmental law is to protect the environment and human health from harm. In recognition of the role that businesses and corporations play in causing such harm, many environmental laws have been amended to criminalize their harmful conduct.

Focusing on the criminal provisions in the Clean Air Act, Clean Water Act, and Resource Conservation and Recovery Act that target offenders who engage in conduct “knowingly,” this Note addresses the split regarding the intent required for a criminal conviction, and the resultant weight accorded these statutes. Courts …

An Immunostimulatory Glycolipid That Blocks Sars-Cov-2, Rsv, And Influenza Infections In Vivo, Moriya Tsuji, Zhenlu Chong, Tamarand L Darling, Kuljeet Seehra, Adrianus C M Boon, Michael S Diamond, Et Al.

2020-Current year OA Pubs

Prophylactic vaccines for SARS-CoV-2 have lowered the incidence of severe COVID-19, but emergence of viral variants that are antigenically distinct from the vaccine strains are of concern and additional, broadly acting preventive approaches are desirable. Here, we report on a glycolipid termed 7DW8-5 that exploits the host innate immune system to enable rapid control of viral infections in vivo. This glycolipid binds to CD1d on antigen-presenting cells and thereby stimulates NKT cells to release a cascade of cytokines and chemokines. The intranasal administration of 7DW8-5 prior to virus exposure significantly blocked infection by three different authentic variants of SARS-CoV-2, as …

Yes-Associated Protein-1 Overexpression In Ocular Surface Squamous Neoplasia; A Potential Diagnostic Marker And Therapeutic Target, Peter Julius, Stepfanie N. Siyumbwa, Fred Maate, Phyllis Moonga, Guobin Kang, Trevor Kaile, John T. West, Charles Wood, Peter C. Angeletti

School of Graduate Studies Faculty Publications

Yes-associated protein-1 (YAP-1) is a Hippo system transcription factor, which serves as an oncogene in squamous cell carcinoma, and several solid tumors when the Hippo pathway is dysregulated. Yet, the activity of YAP-1 in ocular surface squamous neoplasia (OSSN) has not been determined. Here, we investigate the relationship between YAP-1 overexpression and OSSN. Using a cross-sectional study design, we recruited 227 OSSN patients from the University Teaching Hospitals in Lusaka, Zambia. Immunohistochemistry was used to assess YAP-1 protein overexpression in tumor tissue relative to surrounding benign squamous epithelium. OSSN patient samples (preinvasive, n = 62, 27% and invasive, n = …

Fibrosis-The Tale Of H3k27 Histone Methyltransferases And Demethylases, Morgan D. Basta, Svetlana Petruk, Alexander Mazo, Janice L. Walker

Department of Biochemistry and Molecular Biology Faculty Papers

Fibrosis, or excessive scarring, is characterized by the emergence of alpha-smooth muscle actin (αSMA)-expressing myofibroblasts and the excessive accumulation of fibrotic extracellular matrix (ECM). Currently, there is a lack of effective treatment options for fibrosis, highlighting an unmet need to identify new therapeutic targets. The acquisition of a fibrotic phenotype is associated with changes in chromatin structure, a key determinant of gene transcription activation and repression. The major repressive histone mark, H3K27me3, has been linked to dynamic changes in gene expression in fibrosis through alterations in chromatin structure. H3K27-specific homologous histone methylase (HMT) enzymes, Enhancer of zeste 1 and 2 …

Tiam1 Coordinates Synaptic Structural And Functional Plasticity Underpinning The Pathophysiology Of Neuropathic Pain, Lingyong Li, Qin Ru, Yungang Lu, Xing Fang, Guanxing Chen, Ali Bin Saifullah, Changqun Yao, Kimberley F Tolias

Faculty, Staff and Students Publications

Neuropathic pain is a common, debilitating chronic pain condition caused by damage or a disease affecting the somatosensory nervous system. Understanding the pathophysiological mechanisms underlying neuropathic pain is critical for developing new therapeutic strategies to treat chronic pain effectively. Tiam1 is a Rac1 guanine nucleotide exchange factor (GEF) that promotes dendritic and synaptic growth during hippocampal development by inducing actin cytoskeletal remodeling. Here, using multiple neuropathic pain animal models, we show that Tiam1 coordinates synaptic structural and functional plasticity in the spinal dorsal horn via actin cytoskeleton reorganization and synaptic NMDAR stabilization and that these actions are essential for the …

Optimizing The Membrane-Assisted Recovery Of Solids (Mars) Technology For Nutrient Removal And Recovery From Concentrated Waste Streams, Ben Yamin Hoque

USF Tampa Graduate Theses and Dissertations

The effective management of nutrients, such as nitrogen and phosphorus, in decentralizedwastewater treatment environments is a pressing challenge. These nutrients pose significant risks to the environment and public health when discharged into water bodies, while their recovery and reuse can help alleviate nutrient shortages in various industries, including agriculture. Conventional biological treatment methods used in centralized facilities are not suitable for decentralized systems due to operational complexities and space limitations. Chemical treatment mechanisms, such as adsorption, ion exchange, precipitation, and air-stripping, offer potential solutions but are accompanied by challenges like high costs and logistical issues. Therefore, finding a sustainable and …

Defining A Factor-Induced Signaling Signature For Endothelial Tubulogenesis, Sprouting, And Pericyte Recruitment, Prisca Kuanting Lin

USF Tampa Graduate Theses and Dissertations

Endothelial cells (ECs) form the inner lining of blood capillary walls. Our laboratory has developed a serum-free, defined Factor system that allows for human ECs to sprout, form lumens and tube networks, recruit pericytes, and induce capillary basement membrane deposition in 3D extracellular matrices. We have shown that the Factor system drives morphogenesis through the activation of multiple signaling pathways; however, we do not know if all factors in the Factor system are needed to induce maximal EC morphogenesis. If so, we have yet to identify why the Factors are critical to the model other than that they induce activation …

Feasibility And Safety Of Personalized, Multi-Target, Adoptive Cell Therapy (Ima101): First-In-Human Clinical Trial In Patients With Advanced Metastatic Cancer, Apostolia M Tsimberidou, Kerstin Guenther, Borje S Andersson, Regina Mendrzyk, Amir Alpert, Claudia Wagner, Anna Nowak, Katrin Aslan, Arun Satelli, Fabian Richter, Sabrina Kuttruff-Coqui, Oliver Schoor, Jens Fritsche, Zoe Coughlin, Ali S Mohamed, Kerry Sieger, Becky Norris, Rita Ort, Jennifer Beck, Henry Hiep Vo, Franziska Hoffgaard, Manuel Ruh, Linus Backert, Ignacio I Wistuba, David Fuhrmann, Nuhad K Ibrahim, Van Karlyle Morris, Bryan K Kee, Daniel M Halperin, Graciela M Nogueras-Gonzalez, Partow Kebriaei, Elizabeth J Shpall, David Vining, Patrick Hwu, Harpreet Singh, Carsten Reinhardt, Cedrik M Britten, Norbert Hilf, Toni Weinschenk, Dominik Maurer, Steffen Walter

Faculty, Staff and Student Publications

IMA101 is an actively personalized, multi-targeted adoptive cell therapy (ACT), whereby autologous T cells are directed against multiple novel defined peptide-HLA (pHLA) cancer targets. HLA-A*02:01-positive patients with relapsed/refractory solid tumors expressing ≥1 of 8 predefined targets underwent leukapheresis. Endogenous T cells specific for up to 4 targets were primed and expanded in vitro. Patients received lymphodepletion (fludarabine, cyclophosphamide), followed by T-cell infusion and low-dose IL2 (Cohort 1). Patients in Cohort 2 received atezolizumab for up to 1 year (NCT02876510). Overall, 214 patients were screened, 15 received lymphodepletion (13 women, 2 men; median age, 44 years), and 14 were treated with …

Office Of Research, Economic Engagement And Outreach Update, July 5, 2023, Office Of Research, Economic Engagement And Outreach

Office of Research, Economic Engagement and Outreach

No abstract provided.

Endothelial Foxc1 And Foxc2 Promote Intestinal Regeneration After Ischemia-Reperfusion Injury., Can Tan, Pieter R. Norden, Wei Yu, Ting Liu, Naoto Ujiie, Sun Kyong Lee, Xiaocai Yan, Yaryna Dyakiv, Kazushi Aoto, Sagrario Ortega, Isabelle G. De Plaen, Venkatesh Sampath, Tsutomu Kume

Manuscripts, Articles, Book Chapters and Other Papers

Intestinal ischemia underlies several clinical conditions and can result in the loss of the intestinal mucosal barrier. Ischemia-induced damage to the intestinal epithelium is repaired by stimulation of intestinal stem cells (ISCs), and paracrine signaling from the vascular niche regulates intestinal regeneration. Here, we identify FOXC1 and FOXC2 as essential regulators of paracrine signaling in intestinal regeneration after ischemia-reperfusion (I/R) injury. Vascular endothelial cell (EC)- and lymphatic EC (LEC)-specific deletions of Foxc1, Foxc2, or both in mice worsen I/R-induced intestinal damage by causing defects in vascular regrowth, expression of chemokine CXCL12 and Wnt activator R-spondin 3 (RSPO3) in blood ECs …

Investigating Cassava Susceptibility To Xanthomonas Induced Bacterial Blight Using Disease Phenotyping And Genome Engineering Strategies, Kiona Rajene Elliott

Arts & Sciences Graduate Student Theses and Dissertations

In nature, plants regularly contend with bacterial, viral, and fungal pathogens, which employ strategies that promote pathogen fitness and subsequently elicit disease in the host. Xanthomonads are a group of bacterial phytopathogens that induce disease in an extensive range of host plants. Mechanisms underlying pathogen disease promotion have been understudied in food staple crops such as cassava. Cassava is a starchy root crop susceptible to cassava bacterial blight (CBB) a disease caused by the pathogen Xanthomonas phaseoli pv. manihotis (Xpm). Xpm and other bacterial pathogens use effector molecules to manipulate host genes that promote disease susceptibility. Xpm has specialized effector …

Circadian Disruption And Colorectal Cancer Incidence In Black Women, Lauren E Barber, Trang Vopham, Laura F White, Hemant K Roy, Julie R Palmer, Kimberly A Bertrand

Faculty, Staff and Students Publications

BACKGROUND: Animal and experimental studies suggest circadian disruption increases colorectal cancer risk, but evidence in humans is limited. We examined night shift work, chronotype, and residential position within a time zone, proxies for circadian disruption, in relation to colorectal cancer risk.

METHODS: Participants in the Black Women's Health Study, a prospective cohort of 59,000 Black American women established in 1995, reported history of night shift work and chronotype on follow-up questionnaires. Residential position within a time zone was estimated using participant addresses at each questionnaire cycle. Number of colorectal cancer cases and follow-up duration varied by analysis depending on timing …

Estimating Body Weight And Body Condition Score Of Mature Beef Cows Using Depth Images, Yijie Xiong, Isabella C.F.S. Condotta, J. A. Musgrave, Tami M. Brown-Bandl, J. Travis Mulliniks

Department of Animal Science: Faculty Publications

Obtaining accurate body weight (BW) is crucial for management decisions yet can be a challenge for cow–calf producers. Fast-evolving technologies such as depth sensing have been identified as low-cost sensors for agricultural applications but have not been widely validated for U.S. beef cattle. This study aimed to (1) estimate the body volume of mature beef cows from depth images, (2) quantify BW and metabolic weight (MBW) from image-projected body volume, and (3) classify body condition scores (BCS) from image-obtained measurements using a machine-learning-based approach. Fifty-eight crossbred cows with a mean BW of 410.0 ± 60.3 kg and were between 4 …

Morphological Trait Evolution In Solanum (Solanaceae): Evolutionary Lability Of Key Taxonomic Characters, Rebecca Hilgenhof, Edeline Gagnon, Sandra Knapp, Xavier Aubriot, Eric Tepe, Lynn Bohs, Leandro Giacomin, Yuri Gouvea, Andres Orejuela, Christopher T. Martine, Clara Ines Orozco, Iris E. Peralta, Tina Sarkinen

Faculty Journal Articles

Solanum is one of the world's largest and economically most important plant genera, including 1245 currently accepted species and several major and minor crops (e.g., tomato, potato, brinjal eggplant, scarlet eggplant, Gboma eggplant, lulo, and pepino). Here we provide an overview of the evolution of 25 key morphological traits for the major and minor clades of this giant genus based on stochastic mapping using a well-sampled recently published phylogeny of Solanum. The most evolutionarily labile traits (showing >100 transitions across the genus) relate to plant structure (growth form and sympodial unit structure), herbivore defence (glandular trichomes), pollination (corolla shape …

Apologetics And Popular Culture Phenomena: A Critique Of Ted Turnau's Method Concerning Anime, Matthew Hunter Hamilton

Masters Theses

Limited work has been performed in the field of Christian apologetics and popular culture, much less the intersection between anime and Christian apologetics. One scholar who has attempted to broach this gap has been Ted Turnau, who has developed a five-step diagnostic method for apologetically engaging popular culture phenomena. However, his method contains key flaws. Using the work of Thomas Lamarre, a scholar in the field of anime studies, a central contention will be made: that the material and technology of anime have processes that affect how viewers inhabit and orient themselves in the world, which in turn influences their …

Applicability Of Bio-Adsorbents Synthesized From Maize/Corn Plant Residues For Heavy Metals Removal From Aquatic Environments: An Insight Review, Jonas Bayuo, Mwemezi J. Rwiza, Kelvin Mark Mtei

All Rsif Scholars' Publications

The underutilization of agricultural waste products in recent years has resulted in environmental issues owing to improper disposal. As a result, heavy metals removal from aqueous systems utilizing sorbent materials produced from agricultural wastes has received a lot of attention. The current study provides an insightful review of the use of bio-adsorbents synthesized from maize/corn residues to decontaminate various toxicants from wastewater. Although bio-adsorbents made from maize/corn residues have shown to be efficient in the sequestration of heavy metals from wastewater, no study has looked at hybrid bio-adsorbents made from various parts of the maize/corn plant. Moreover, all studies practically …

Occupational Therapist Perceptions On How Eye Movement Therapy Supports Working Memory In Children Diagnosed With Attention Deficit/Hyperactivity Disorder, Latres M. Bell

Doctoral Dissertations and Projects

Previous research reports that children with attention-deficit/hyperactivity disorder (ADHD) show a deficit in their working memory. Working memory is the ability to temporarily manipulate, store, and retrieve information during cognitive tasks. Working memory is considered a critical deficiency in individuals diagnosed with ADHD. Working memory has been shown to be a mechanism for symptoms of disorganization and inattention in individuals diagnosed with ADHD. This qualitative research study reviewed occupational therapists’ perceptions on their role working with children, aged six to 10 years old, diagnosed with ADHD and low working memory, who have had eye movement therapy. The occupational therapists’ perceptions …

It’S Black And White: An Investigation Into The Founding Of Three Post-Civil War Black Colleges, Melvin Gamble Miller

Doctoral Dissertations and Projects

This work examines how White and Black Baptist worked together to form and developed three historically Black Colleges immediately after the Civil War in the most contested region of the Eastern seaboard—Shaw University, Virginia Union University and Benedict College. After the Civil War, there were different schools of thought on how to fill the void in African American education. Ex-slaves, Radical southerners, Freemen, Northern Baptist, Black Baptist, Southern Baptists, and missionary organizations from various denominations all offered varying perspectives on what forms of educations were relevant for the newly emancipated slave. Some held to the notion that industrial education was …

The Relevance Of Gender For Excellent Research And Innovation: An Opinion, Yvonne Galligan

Irish Journal of Academic Practice

This piece argues that the integration of sex and/or gender in research improves the quality of the research, contributes to excellence in the research process and makes the outcomes beneficial to society.