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The Effects Of Diet-Induced Obesity On Cerebral Microvascular Bioenergetics, Alec Anthony Hanshew

Graduate Theses, Dissertations, and Problem Reports (ETD)

Obesity is a worldwide epidemic but is especially a concern in the United States with over 40% of the population being obese. Obesity is known to cause increased metabolic demand, systemic inflammation, peripheral vascular dysfunction, and increased levels of oxidative stress and reactive oxygen species (ROS). However, the role of obesity on cerebrovascular bioenergetic function has not been fully elucidated. This study aimed to determine the impact of diet-induced obesity on cerebrovascular bioenergetics. 72, 6-month-old C57Bl/6 male and female mice were randomized into control and HFD groups. Control mice were subjected to the feeding of standard chow (18% fat, 24% …

Astrocyte Reprogramming By Th17 Cells In Autoimmune Neuroinflammation: Mechanisms And Implications, Sarah Marie Milne

Graduate Theses, Dissertations, and Problem Reports (ETD)

Multiple sclerosis (MS) is a cell-mediated, demyelinating autoimmune disease that affects the central nervous system (CNS). T helper (Th) 17 cells mediate the pathogenesis of MS and its animal model, experimental autoimmune encephalomyelitis (EAE), in part by infiltrating the CNS and interacting with astrocytes, a resident glial cell. Astrocytes are central to coordinating several inflammatory processes in the CNS and can respond to cytokines and chemokines produced by encephalitogenic Th17 cells. Pathogenesis of MS and EAE requires the infiltration of immune cells from the periphery, including autoreactive T cells, B cells, monocytes, and neutrophils, that lead to neuroinflammation, demyelination, and …

Endogenous Glucocorticoid Signaling Is Required For Normal Macrophage Response To H. Pylori Infection, Stuti Khadka

Graduate Theses, Dissertations, and Problem Reports (ETD)

Gastric cancer is the fifth leading cause of cancer-related deaths worldwide, affecting millions of people. H. pylori is the strongest risk factor for gastric cancer, with about 90% of the cases strongly associated with H. pylori infection. Half of the world's population is infected with this bacterium. However, only a small subset of the infected population (1-3 %) go on to develop gastric cancer. Diagnosis of the disease at an early stage is the biggest challenge because of the widespread prevalence of H. pylori infection and a long asymptomatic phase before the advanced stages. Patients are left with limited treatment …

Measures Of Feed Efficiency In Beef Cattle: Biological Basis And Effect On Response To Dietary Supplementation, Modoluwamu Idowu

Graduate Theses, Dissertations, and Problem Reports (ETD)

During the feedlot receiving period, newly weaned beef cattle face various stressors, including weaning, transportation, vaccination, and exposure to pathogens. Additionally, changes in diet and low dry matter intake contribute to nutrient deficiencies, heightening susceptibility to disease and performance declines. Therefore, we examined the effects of dietary supplementation with a multicomponent blend of prebiotics and probiotics on the health, immune status, metabolism, and performance of newly weaned beef steers during a 35-day receiving period. Our results showed that compared to the control group, the supplemental additive (SYNB) increased average daily gain (ADG), dry matter intake, and meal events during the …

Synaptic Zinc Transporters Support Normal Synapse Structure And Function In Mouse Auditory Cortex, Abbey Manning

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many regions of the brain are highly enriched with free zinc inside glutamatergic vesicles at presynaptic terminals. At these synapses, zinc transporter 3 (ZnT3) moves zinc into vesicles where it is co-released with glutamate. Alterations in ZnT3 are implicated in multiple neurodevelopmental disorders, and ZnT3 knock-out (KO) mice – which lack synaptic zinc – show behavioral deficits associated with autism spectrum disorder and schizophrenia. Shank3 is a synaptic scaffolding protein that assists in tethering and organizing structural proteins and glutamatergic receptors in the postsynaptic density of excitatory synapses. The localization of Shank3 at excitatory synapses and the formation of stable …

The Effects Of Forgetting And Disclosure On Mock Jurors' Perceptions Of An Alleged Rape, Johnathan Meier

Graduate Theses, Dissertations, and Problem Reports (ETD)

Memory recall and forgetting is a complex process. Memories can be completely forgotten (e.g., Ebbinghaus, 1885/1964; Murre, 2015) or repressed and recovered at a later time (e.g., Key et al., 1996; Foster-Lee et al., 1999). Instances of sexual assault may be disclosed to a close acquaintance or medical professional at the time of the event or kept secret until the time of reporting to authorities (e.g., Pica et al., 2020a). In the current study, mock jurors’ perceptions of credibility and guilt, as well as the amount of compensation awarded to the plaintiff, will be assessed in response to testimony depicting …

An Inhibitory Recurrent Network In The Olfactory System, Farzaan Salman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neuromodulatory circuits are a necessary component of neural flexibility as they are able to act differentially depending on the receptors expressed by their downstream targets. Identifying the synaptic partners of modulatory neurons and determining the neurotransmitter and receptor content of these partners is necessary to identify the rules governing neuromodulatory circuits. In this study, I characterized the circuit of the contralaterally projecting, serotonin-immunoreactive deutocerebral neurons (CSDns), the sole synaptic source of the neuromodulator serotonin in the olfactory circuit of Drosophila melanogaster, by identifying the strongest synaptic partners of the CSDns, and the expression of genes encoding neurotransmitters and receptors of …

Sars-Cov-2 Immunogenicity Models Inform Novel Strategies That Can Improve Mrna Vaccines, Katherine S. Lee

Graduate Theses, Dissertations, and Problem Reports (ETD)

The onset of the 2020 COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, issued a call to action in the fields of biomedical science and public health to implement strategies to contain and protect against the viral threat. In record times, pharmaceutical companies produced antiviral therapies and novel mRNA vaccines that were approved by the FDA for human use. With time, these vaccines were able to assist in ending the pandemic. However, time also showed the limits of mRNA vaccine induced immunity. Despite antibody responses being initially robust, titers dramatically wane with time and are limited in cross-neutralizing ability. As …

Investigation Of L-Serine Synthesis And Transport As Stabilizing Feedback Mechanisms Within The Tsetse-Wigglesworthia Symbiosis, Ryan Phillips

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tsetse flies (Glossina morsitans), which are the obligate vectors of Old-World trypanosomes (Trypanosoma spp.) that cause African trypanosomiasis, have a bacteriome that houses the intracellular endosymbiont Wigglesworthia glossinidia. The ancient coevolution of these species has led to the integration of Wigglesworthia metabolism into tsetse physiology and a subsequent reliance on nutrient exchange for maintaining the fitness of both partners. The establishment of this obligate mutualism has facilitated the emergence of feedback mechanisms that stabilize the symbiosis, such as that which involves the exchange of L-serine and B vitamins. The Gammaproteobacteria Wigglesworthia supplement B vitamins lacking …

A Legacy Of Labor: Maternity Narratives In 1960s And 1970s North American Life Writing, Katelynn Ann Vogelpohl

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

A Legacy of Labor: Maternity Narratives in 1960s and 1970s North American Life Writing

Katelynn Ann Vogelpohl

The phenomenon of maternity has been repeatedly described as an event that shakes the very foundations of social and physical identity. As the flesh of the pregnant person literally divides to produce new life, one subject becomes enclosed within another, dramatically affecting the pregnant person’s sense of self and causing a confluence of intense, and often conflicting, feelings. In North America, there are two dominant, and seemingly opposing, discourses on pregnancy and childbirth: the institutional medical discourse and the natural childbirth discourse. …

Adverse Childhood Experiences Predict Mortality Risk: The Role Of Social Support & Social Strain, Meredith A. Willard

Graduate Theses, Dissertations, and Problem Reports (ETD)

Childhood adversity has long-lasting negative effects across the lifespan including increased mortality risk. The love and support individuals receive from others, also known as social support, has shown to be a protective factor against ACEs. However, little research has investigated the amplifying effects of social conflict and strain that often accompanies social relationships. Utilizing data from the Midlife Development in the U.S. (MIDUS) study, I tested whether higher levels of social support would buffer the negative effects of adverse childhood experiences on mortality risk, and whether higher levels of social strain would amplify these associations. The sample included 6,150 participants …

Bioenergy Cropping Systems Established On Marginal Lands: Effects Of Land Use, Energy Crop, And Fertility Amendments On Soil Health, Mica M. Keck

Graduate Theses, Dissertations, and Problem Reports (ETD)

Bioenergy crops offer a promising strategy for renewable energy production, accompanied by carbon (C) storage benefits when managed sustainably. Utilizing marginal lands for energy feedstock production presents an opportunity for bioenergy generation and concurrent climate mitigation without competing with food crops. However, achieving optimal yields requires strategies to restore soil fertility, which require increased understanding of the interactive effects of prior land use, energy crop species, and fertility amendments available. This study, conducted as part of the Mid-Atlantic Sustainable Biomass (MASBio) Consortium, investigated the effects of biochar incorporation—a C-rich charcoal derived from biomass, intended for soil enhancement—on bioenergy cropping systems …

Analysis Of Mutations In Protein Kinase Ck2 Linked To Okur-Chung’S Neuropathy, Shivani Maan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The studies described here focus on mutations in protein kinase CK2 linked to ‘Okur-Chung’s Neuropathy’ (OCN), a neurodevelopmental disorder that involves 10 distinct mutations in the catalytic subunit CK2α. It has, however, remained unresolved if these mutations impair CK2α activity or if they also impair association with the regulatory CK2β subunit, which is necessary for assembly of the α2β2 holoenzyme. I describe efforts to resolve these questions for two mutations, Y50S and D175G. The former is thought to reside at the CK2α-CK2β interface, whereas the latter is located proximal to the active site. I therefore hypothesized that Y50S should perturb …

(Non-) Recovery Of An Agricultural Stream From Straightening And Dredging, Aras Anderson Mann

Graduate Theses, Dissertations, and Problem Reports (ETD)

In recent history, natural, meandering streams have been straightened and dredged to reduce flooding. While this practice can be effective in reducing flooding locally, it often results in the degradation of stream water quality and aquatic ecosystems. A straighter channel inherently increases the stream gradient, which could increase flow velocity, shear stress, and potentially downstream sediment yield. Studies have shown that straightened, channelized streams often begin to return to a meandering pattern 35-50 years post-channelization. Yet cross-sectional surveys and air photo analysis of the stream reach in this study, Deckers Creek, indicate little to no observable trend of the stream …

Community Resource Programs Impact On Marginalized Communities Access To Health Care Services: A Program Development Project, Alexis N. Foehrkolb

Graduate Theses, Dissertations, and Problem Reports (ETD)

Caring for children and meeting their needs comes with a variety of considerations regarding temperament, developmental progress, and socioeconomic impact. For children with special healthcare needs (CSHCN), those considerations become more complex. Community resource programs are designed to help families of CSHCN get access to the resources they need regarding general and healthcare services. A program was developed in collaboration with Parents Place of Maryland (PPMD) to create materials effective in educating parents of CSHCN on desired topics of interest, create a virtual environment where parents felt safe asking questions regarding specific topics of interest, and determine the most effective …

Estradiol’S Role In Timely Puberty Onset In The Ewe, Eliana Gerardine Aerts

Graduate Theses, Dissertations, and Problem Reports (ETD)

In female sheep, the release of gonadotropin-releasing hormone (GnRH) from the hypothalamus triggers luteinizing hormone (LH) to be released from the pituitary. Luteinizing hormone, in turn, acts on the ovaries to elicit estradiol (E₂) release. E₂ acts in a negative feedback manner to control the amount of LH released. The increase in LH release that drives puberty onset results from a decrease in sensitivity to E₂ as the animal ages. However, the neural mechanisms mediating this change in sensitivity are not known, as is where in the hypothalamus E₂-negative feedback occurs. In the arcuate …

Transparent And Conductive Gallium Oxide Electrode For Simultaneous Recording And Optogenetic Stimulation, Christopher Patrick Carey

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neural electrode technology has been around for centuries since the times of Galvani. In early electrophysiology experiments metal wires were used to induce contractions in dissected animals. The metal wire electrode has since been a standard tool to both stimulate and record neural activity. In the past two decades, a new strategy for neural stimulation has been formulated based on the emergent field of optogenetics. Optogenetics refers to the use of light-sensitive proteins genetically imbedded in the membrane of a neuron to elicit neural activity. This technique offers more selectivity in the stimulation of neurons. Typical optogenetic neural electrodes, or …

Comparison Between Coronally Advanced Flap With Sub-Epithelial Connective Tissue Graft And Tunnel Technique With Sub-Epithelial Connective Tissue Graft In Achieving Mean Root Coverage At Multiple Recession Defects: A Retrospective Study, Christian Daniel Davila

Graduate Theses, Dissertations, and Problem Reports (ETD)

Aim: The primary objective is to retrospectively compare the mean root coverage (MRC) achieved using a coronally advanced flap and sub-epithelial connective tissue graft (CAF + CTG) with that achieved using the tunnelling technique and sub-epithelial connective tissue graft (TUN + CTG) for the treatment of multiple recession defects. The secondary objectives are to compare the proportion of complete root coverage (CRC) achieved with CAF + CTG compared to TUN + CTG, compare the proportion of CRC achieved in these two techniques with respect to level of training (American board-certified periodontist or Graduate periodontics residents) and to evaluate the …

Women In Extension Persevering In Leadership Roles, Lexis Danielle Trickett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the last several decades, the rates at which women have been pursuing higher levels of education have steadily increased. However, there is a discrepancy between the number of women graduating, and the amount entering the workforce, specifically in leadership roles. The purpose of this study was to share a current profile of women in the West Virginia Cooperative Extension Service by allowing them to describe their career journeys, supports and barriers they may or may not have faced, and mentoring experiences. By using a non-experimental quantitative methodology, a population of male and female Extension Agents and Specialists were surveyed …

Characterization And Evaluation Of Various Biochar Types As Green Adsorbents For Rare Earth Element Recovery From Aqueous Solutions, Oluwaseun Victor Famobuwa

Graduate Theses, Dissertations, and Problem Reports (ETD)

Rare earth elements (REEs) are members of the lanthanide family (atomic number 57 – 71). They are significantly important to the global economy due to their applications in renewable energy, defense, and medical industries. REEs are primarily derived from bastnaesite and monazite but may also be present in xenotime, cerite, alanite, and many other types of mineralization in lesser amounts. Due to the increasing demand for REEs and their criticality in the supply chain, the need to explore secondary sources of REEs has gained tremendous importance.

Secondary sources of REEs include but are not limited to acid mine drainage (AMD), …

Characterization Of Host Cell Specific Interactions During Spotted Fever Group Rickettsiae Infection, Emerson Towey

Graduate Theses, Dissertations, and Problem Reports (ETD)

Vector-borne diseases have been increasing over the past few years due to changes in climate and disturbances in the vectors’ natural environment. To mitigate the risks of these diseases, it is necessary to understand pathogen biology and how it interacts with a host to cause disease. Here we focus our attention on the tick-borne pathogen, Rickettsia rickettsii. R. rickettsii is a member of the spotted fever group (SFG) Rickettsiae and is the etiological agent of Rocky Mountain spotted fever (RMSF), a fatal human disease that is lacking an effective vaccine. Members of the bacterial genus Rickettsia are obligate intracellular endoparasites …

A Podcasting Series On The Historical Event Of Hawk’S Nest Tunnel Disaster, Hannah G. Morgan

Graduate Theses, Dissertations, and Problem Reports (ETD)

This professional project will look into the Hawk’s Nest Tunnel Disaster – a disaster that was birthed due to extreme capitalism and exploitation during the 1930s. This project will explore historical documents, books and interviews with those who are passionate about the event. With that research material, I will produce an educational narrative podcast with a cohesive storyline detailing the event, including a beginning, middle and end. The podcast series will be uploaded to Apple Podcasts and Spotify. This professional project can be replicated and used for other similar projects.

4d Epr Oximetry Imaging For 4d Bioprinting And Tissue Engineering, Sajad Sarvari

Graduate Theses, Dissertations, and Problem Reports (ETD)

Bioprinting, akin to 3D printing, employs cell-laden hydrogels such as GelMA (Gelatine Methacrylate) and Alginate instead of plastic or resin to create biological structures. The printing process followed by subsequent tissue maturation is known as 4D bioprinting, where the fourth dimension is time. Ensuring adequate and consistent oxygen, O2, supply within 3D bioprint volume over the entire maturation period process is crucial, as even transient hypoxia can impact cellular behavior and phenotypes. However, printing of fully developed vasculature remains an unresolved technical challenge. Several approaches to chemically (peroxides) or biologically (photosynthesis) generate oxygen during bioprint maturation have been proposed. However, …

An Evaluation Of A Human-Operant Effort Manipulation And Effects Of Effort Disparity On Renewal, Haleh Amanieh

Graduate Theses, Dissertations, and Problem Reports (ETD)

The relative effort of target and alternative responses during treatments using differential reinforcement of alternative behavior may impact the likelihood that a previously reduced target response will reemerge following a context change (i.e., “renewal”). The purpose of this study was to evaluate the role of an effort disparity between target and alternative responses in a human-operant arrangement. Eighteen college students clicked on one (Experiment 1) or two (Experiment 2) circles moving on a computer screen for points. In Experiment 1, the speed of the circle was manipulated as an index of effort such that three circle speeds (i.e., 50, 100, …

Assessing The Effectiveness Of Crime Scene Measuring Techniques, Lauren M. Fagan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Crime scene mapping is the process of measuring and fixing items present in the crime scene to obtain a detailed to-scale map of the scene as found by the crime scene investigators. When bloodstain patterns are present, investigators must analyze the bloodstains to assist in determining where the blood-shedding event occurred. The volume of origin is determined through a manual stringing method that is labor-intensive and destructive.

Technological advancements have been made in other fields, such as land surveying, and were adopted by law enforcement-related fields. These advancements include electronic terrestrial scanners, like the total station, and three-dimensional scanners, like …

Have You Been To Orlando?: In Relation To The Regulation Of Self-Preferencing In Antitrust Law, Eunkwang Ha

South Carolina Journal of International Law and Business

As a mother of two and a South Korean professional studying abroad in the United States, one of my top priorities for our family's time here was to visit Orlando. My children had endured quite a bit of disruption as I pursued my professional goals, and I felt visiting Disney World and Universal Studios would be a bit of recompense. Although I wanted to use a package deal from a Korean travel agency, it was not a good choice because I needed to stay at hotels connected to Disney World and Universal Studios.

Due to the self-preferencing of Disney World …

Baker's Novelty Plays, Script, Boston, Massachusetts, 1894

Documents

Baker's Novelty Plays script for "The District School at Blueberry Corners, A Farcical Entertainment in Three Scenes" by Laura M. Parsons, 22 pages, Walter H. Baker Company, Boston, Massachusetts, 1894. Signed by Walter H. Baker.

St. James Theatre, Playbill, New York, New York, 1937

Documents

St. James Theatre, playbill for "King Richard II." by William Shakespeare, starring Maurice Evans and Ian Keith, staged by Margaret Webster, New York, New York, beginning 1937 March 8.

Theatre Memphis, Program, Memphis, Tennessee, 1989

Documents

Theatre Memphis, program for "Big River, The Adventure of Huckleberry Finn," music and lyrics by Roger Miller, book by William Hauptman, directed by Sherwood Lohrey, Memphis, Tennessee, 70th Anniversary Mainstage Season, 1989 September 7-24.

Magazine Article, "Beale Street Sings The Blues No More," From The Downtowner, Memphis, Tn, November 1993

Documents

Magazine article, "Beale Street Sings The Blues No More," by Paul Gerald, The Downtowner, Memphis, TN, Vol. 3, Issue 10, November 1993.