Digital Commons Network^™

Opening The Black Box: Carbon-Footprint Calculators, Meat Consumption, And The “Wicked Problem” Of Metric Governance, E. Melanie Dupuis, Dustin Mulvaney

Faculty Research, Scholarly, and Creative Activity

Carbon-footprint calculators are widely used as communication and education tools, designed to make people aware of the ways their personal actions contribute to greenhouse-gas emissions. However, they have come under criticism for emphasizing individual behavior versus structural change. We extend this critique to argue that carbon-footprint calculators are a form of “metric governance” that not only disciplines users but also constrains their abilities to engage with the complexities of climate change and social action. We focus on a particularly wicked problem of carbon-metric governance: livestock greenhouse-gas emissions. We show that carbon-footprint calculators black box and fix the complex politics and …

Revolutionizing Psoriasis Topical Treatment: Enhanced Efficacy Through Ceramide/Phospholipid Composite Cerosomes Co-Delivery Of Cyclosporine And Dithranol: In-Vitro, Ex-Vivo, And In-Vivo Studies, Sammar Fathy Elhabal, Nashwa Abdelaal, Saeed A S Al-Zuhairy, Mohamed Fathi Mohamed Elrefai, Mohamed Mansour Khalifa, Mohammad Ahmad Khasawneh, Ahmed Mohsen Elsaid Hamdan, Passant M Mohie, Rania A Gad, Soad L Kabil, Mohamed Kandeel El-Ashery, Bhaskara R Jasti, Nahla A Elzohairy, Nehal Elfar, Tayseer Elnawawy, Fatma E Hassan, Mohamed Ahmed El-Nabarawi

Faculty, Staff and Students Publications

PURPOSE: Improving the treatment of psoriasis is a serious challenge today. Psoriasis is an immune-mediated skin condition affecting 125 million people worldwide. It is commonly treated with cyclosporine-A (CsA) and dithranol (DTH). CsA suppresses the activation of T-cells, immune cells involved in forming psoriatic lesions. Meanwhile, DTH is a potent anti-inflammatory and anti-proliferative drug that effectively reduces the severity of psoriasis symptoms such as redness, scaling, and skin thickness. CsA and DTH belong to BCS class II with limited oral bioavailability. We aim to develop a drug delivery system for topical co-delivery of CsA and DTH, exploring its therapeutic potential. …

Standing And Snitches, Emily Strauss

Faculty Scholarship

No abstract provided.

Generation And Characterization Of In Vitro Transcribed Mrna, Cristina Poveda, Yi-Lin Chen, Ulrich Strych

Faculty, Staff and Students Publications

Here we describe the in vitro preparation of mRNA from DNA templates, including setting up the transcription reaction, mRNA capping, and mRNA labeling. We then describe methods used for mRNA characterization, including UV and fluorescence spectrophotometry, as well as gel electrophoresis. Moreover, characterization of the in vitro transcribed RNA using the Bioanalyzer instrument is described, allowing a higher resolution analysis of the target molecules. For the in vitro testing of the mRNA molecules, we include protocols for the transfection of various primary cell cultures and the confirmation of translation by intracellular staining and western blotting.

A Far-Field Radio Frequency Experimental Exposure System For Yeast: Creating A New Standard, Soraya Nevin

Electrical and Computer Engineering

This paper presents a framework for investigating the potential effects of radiofrequency (RF) exposure on gene expression and cellular processes. Utilizing Schizosaccharomyces Pombe (S. pombe) as a model organism and specialized equipment to create a standard test environment for continued research in this field. Key methods include environmental monitoring, RF antenna configuration, and power density mapping. Results demonstrate the feasibility of the proposed experimental setup. Future directions involve variations in RF frequency and exposure time, as well as exploring underlying molecular mechanisms. This framework aims to lay the groundwork for future experimentation in this field. It emphasizes the need for …

Leveraging Genomics And Transcriptomics For Gene Discovery In Dry Pea, Josephine Johnson

Genomics and Bioinformatics

Over the past two decades, there has been a significant increase in the utilization of DNA marker-based mapping studies to genetically map and further improve complex quantitative traits. A major caveat of this approach is that genetic mapping of the underlying genes conferring target phenotypes is challenging often due to the extent of long-range linkage disequilibrium (LD) in the genome, particularly in self-pollinated crops. Recent technologies allow us to examine expression-phenotype associations using transcriptome-wide association studies (TWAS) which is independently affected by LD, unlike in the case of genetic markers. This is of greatest utility in species where linkage disequilibrium …

From Pages To Places: Situating Kelly Et Al.’S The Robot Zoo: A Mechanical Guide To The Way Animals Work In Real-Life Context, Nasih Alam

English

The Robot Zoo: A Mechanical Guide to the Way Animals Work (1994), written by John Kelly, Dr. Philip Whitfield and Obin, consists of textual descriptions and visual images of sixteen robot animals. Among them, my discussion of Giraffe, Platypus and Rhino highlights the fact that they are endangered animals who are facing extinction due to climate change, human encroachment, poaching, illegal trade, and a lack of biodiversity. Although my other subjects of discussion such as House Fly, Grasshopper, Chameleon and Bat do not necessarily face any immediate extinction, this paper shows how scientists are using the heights and zips of …

The Price Of Criminal Law, Russell M. Gold

Articles

Should tax dollars pay for more criminal law, better public schools, or a new community center? Different counties will answer the question differently, but facing these tradeoffs is profoundly important to democratic governance. Nonetheless, because the criminal legal system diffuses power and hides and offioads costs, officials and voters do not have to honestly consider that question. These structural features place a hidden thumb on the scale that gives counties more criminal enforcement than they pay for. That is a problem. Too much enforcement is particularly pernicious in criminal law: Incarceration inflicts tremendous suffering, especially in poor communities of color. …

Technological Advancements In Brunei’S Local Agriculture And Fisheries Companies: An Exploratory Study Based On Field Observations, Muhammad Firdaus Mujibuddin Syah Mustafa, Navaranjan Namasivayam, Hong Shyang Pei, Fatnin Farhanah Alihan, Amni Sahilah Sahrullamzah, Khairina Umairah Takiyaudin

ASEAN Journal on Science and Technology for Development

This exploratory study, through seven field observations and interviews, investigates the technological advancements transforming Brunei's agricultural landscape in pursuit of local production augmentation and self-sufficiency. It confronts challenges with emerging technologies like aquaponics, hydroponics, fertigation systems, closed-house poultry systems, and UV lighting, which hold promise for enhanced farming practices. The research provides a comprehensive assessment of current practices, including livestock management advancements, underscoring their potential to boost productivity, optimize resource use, and promote sustainable agriculture. Furthermore, it discusses the broader implications for industry standards, job employability, and alignment with Brunei's Vision 2035. The findings contribute invaluable insights, advocating for strategic …

Physicochemical Characterization Of Edible Insect Oils: Insights Into Fatty Acid Composition, Thermal Behavior And Quality Parameters, Eng-Tong Phuah, Yee-Ying Lee, Teck-Kim Tang, Guanghui Li, Shyang-Pei Hong, Syazana Abdullah Lim

ASEAN Journal on Science and Technology for Development

Edible insects are emerging as a sustainable food source due to their high nutritional value. This study employed the three-phase partitioning (TPP) method to extract oils from sago worm (Rhynchophorus ferrugineus), superworm (Zophobas morio), and cricket (Acheta domesticus). The study evaluated and compared the physicochemical properties of these insect oils, including iodine value (IV), peroxide value (PV), p-anisidine value (p-AV), acid value (AV), refractive index (RI), thermal behavior, and fatty acid compositions. Results revealed that the sago worm exhibited the highest oil yield (47.8 ± 2.2%) compared to the superworm (23.8 ± 1.4%) and cricket (6.4 ± 0.6%). Cricket oil …

Assessing The Organic Acids-Solubilized Collagen Derived From The Skin Of Unicornfish (Naso Reticulatus Randall, 2011) On Physicochemical And Structural Characteristics: Toward Alternative Source For Industrial Applications, Dayang Fazrianie Harianto, Abdul Aziz Jaziri, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md. Noordin, Masmunira Rambli, Nurul Huda

ASEAN Journal on Science and Technology for Development

Converting skin by-product of unicornfish (Naso reticulatus Randall, 2011) into a valuable commodity like collagen is a good strategy to explore alternative sources for land-based animals. Additionally, marine fish collagens are preferable due to their safer and great properties. This project aimed to compare extractability of collagens prepared with three organic acids (i.e., acetic, lactic and citric acids), and their characteristics were also discussed. Collagen extracted with citric acid (CSCUS) (7.10%) had a significantly higher yield (p

Antioxidant And Antimicrobial Properties Of Yellow Mealworm (Tenebrio Molitor) Protein Concentrates And Hydrolysates Derived From Varied Dietary Regimens, Shyang-Pei Hong, Eng-Tong Phuah, Wey-Lim Wong, Yee-Ying Lee, Teck-Kim Tang, Ali Yassoralipour

ASEAN Journal on Science and Technology for Development

The larval stage of the darkling beetle, Tenebrio molitor, often referred to as the yellow mealworm, has received significant attention as a promising source of high-quality protein for human consumption due to its exceptional nutritional profile. This study focused on assessing the antioxidant and antimicrobial activities of protein concentrates and hydrolysates obtained from mealworm specimens raised under distinct dietary conditions. One cohort of mealworms was sustained on food waste comprising rice bran, milled corn, and carrots (self-bred) (SM), while another group was exclusively provided with rice bran (commercially fed) (CM). Enzymatic hydrolysis of mealworm protein was accomplished using protease enzymes …

Assessment Of Novel Plant-Based Fat Efficiency In Comminuted Chicken Meat System, Salma Mohamad Yusop, Shu Jo Lin, Nurkhuzaiah Kamaruzaman, Nurlisa Mohd Azhari

ASEAN Journal on Science and Technology for Development

The composition of fats and oils significantly impacts the flavour, nutrition, and appearance of food. Excessive consumption of saturated fatty acids, primarily found in animal fats, raises the risk of coronary heart disease. To address this, plant-based fats are used as substitutes, with microalgae offering unique nutritional benefits as a fat source. This study evaluates the performance of structured plant-based fats, incorporating microalgae (Aurantiochytrium sp. SW1), as a fat substitute in chicken patties. Four formulations were tested: 0% (control), 5%, 7%, and 10% structured microalgae-based fat. Colour, texture, water-holding capacity, cooking loss, and microstructure in both raw and cooked samples …

Nutritional Quality Of Cultured Giant Freshwater Prawns (Macrobrachium Rosenbergii), Nurul F.M.S. Rizal, Najeebah A. Tashim, Zuhairi Azahari, Aida M. Basri, Tiong H. Lim, Aqilah Junaidi, Suriayati Chuprat, Seno A. Putra, Hanif Fakhrurroja

ASEAN Journal on Science and Technology for Development

Macrobrachium rosenbergii, or the giant freshwater prawn has high market demand due to its exceptional nutritional value for the human diet. Often, most nutritional studies focus on the edible prawn parts, rather than the prawn discarded components. The nutritional evaluation of different prawn parts can provide insights into the nutrient composition and guiding for the development of value-added products. Three groups of prawn samples were collected from 3 different ponds, representing different prawn sizes. The water quality analysis of each pond was also conducted to determine the extent to which it can affect the nutritional composition. The conventional and biochemical …

Innovative Approaches And Challenges In Brunei's Agricultural Management, Nuramalina Manshor, Ulaganathan Subramanian

ASEAN Journal on Science and Technology for Development

The management and development in agriculture attract agricultural economic science for the pricing, business, farm policy, and financial knowledge. It conjointly draws on plant and animal sciences for soil, seed and fertilizer information, weed control, insect management, and rationing and breeding; agricultural engineering knowledge for farmhouses, vehicles, irrigation, field drying, drainage, and management of erosions; and data on human behavior in Science and Social Science.

The Influence Of Financial Literacy, Environmental Literacy, And Environmental Concern On Green Preferences Among Young Adults In Brunei Darussalam, Siti Rafhanah Binti Haji Nor Hasnul, Shaista Wasiuzzaman

ASEAN Journal on Science and Technology for Development

This study aims to assess the green preferences in Brunei Darussalam among young adults evaluate the level of environmental and financial literacy among Brunei’s young adults and investigate the influence of financial literacy, environmental literacy and environmental con- cern on the green preferences of young adults in Brunei. A survey questionnaire was distrib- uted to youths between the ages of 15 to 40 years old, out of which 192 responses were gath- ered. Various statistical analyses were carried out to achieve the aims. It is found that green preferences were moderate among Bruneian young adults. Environmental literacy is found to …

Implementation Of A Low-Cost Electronic Speed Controller For A Low-Voltage Three- Phase Induction Motor In A Reused Vehicle, Keneth Brayan Perez Huaroc, Ronald Javier Mendoza Bonifacio, Moises Carlos Tanca Villanueva

ASEAN Journal on Science and Technology for Development

This article presents the design and implementation of a low-cost electronic speed controller (ESC) for a three-phase low voltage induction motor in a reused vehicle. The proposed ESC is based on the Pulse Width Modulation (PWM) technique, which is a widely used method for motor speed control. The system is built using electronic components available in the Peruvian market and has been tested on a prototype vehicle. The results show that the ESC provides control of the motor speed. The proposed system is a low-cost alternative to commercial ESCs, and it can be used in various applications where control of …

Temporal Lobe Epilepsy And Obesity In Adults, Mahmoud Fathy Saleh, Mohammed Abbas, Mostafa Mansour, M. Saad

Mansoura Medical Journal

Background: Epilepsy is a common, chronic, and disabling neurologic state. Medial temporal lobe epilepsy (TLE) is the commonest, and best-defined, form of symptomatic localization-related epilepsy. Obesity-induced mitochondrial dysfunction and oxidative stress are thought to participate in the initiation and progression of epilepsy. Obesity is a potentially preventable predisposing factor for drug resistant epilepsy (DRE).

Objective: the aim of this study was to investigate the association of TLE with obesity and the effect of obesity on seizure control.

Methods: This case control study was carried out on 50 patients with TLE and was divided into two groups, …

An Overview Of The Effects Of Tenapanor On Visceral Hypersensitivity In The Treatment Of Irritable Bowel Syndrome With Constipation, Prashant Singh, Gregory S Sayuk, David P Rosenbaum, Susan Edelstein, Kenji Kozuka, Lin Chang

2020-Current year OA Pubs

BACKGROUND: Patients with irritable bowel syndrome with constipation (IBS-C) experience persistent abdominal pain, a common symptom leading to greater healthcare utilization and reports of treatment non-response. Clinically significant improvements in abdominal pain were observed in clinical trials of tenapanor, a first-in-class inhibitor of sodium/hydrogen exchanger isoform 3 (NHE3), for the treatment of IBS-C in adults.

AIM: This narrative review reports the current knowledge about visceral hypersensitivity as a mechanism for abdominal pain in patients with IBS-C and explores the published evidence for hypothesized mechanisms by which tenapanor may reduce visceral hypersensitivity leading to the observed clinical response of decreased abdominal …

Neurosteroids Mediate And Modulate The Effects Of Pro-Inflammatory Stimulation And Toll-Like Receptors On Hippocampal Plasticity And Learning, Yukitoshi Izumi, Kazuko A. O'Dell, Anil G. Cashikar, Steven M. Paul, Douglas F. Covey, Steven J. Mennerick, Charles F. Zorumski

2020-Current year OA Pubs

Pro-inflammatory changes contribute to multiple neuropsychiatric illnesses. Understanding how these changes are involved in illnesses and identifying strategies to alter inflammatory responses offer paths to potentially novel treatments. We previously found that acute pro-inflammatory stimulation with high (μg/ml) lipopolysaccharide (LPS) for 10-15 min dampens long-term potentiation (LTP) in the hippocampus and impairs learning. Effects of LPS involved non-canonical inflammasome signaling but were independent of toll-like receptor 4 (TLR4), a known LPS receptor. Low (ng/ml) LPS also inhibits LTP when administered for 2-4 h, and here we report that this LPS exposure requires TLR4. We also found that effects of low …

Control Of Acute Myeloid Leukemia And Generation Of Immune Memory In Vivo Using Amv564, A Bivalent Bispecific Cd33 X Cd3 T Cell Engager, Linda G. Eissenberg, Julie K. Ritchey, Michael P. Rettig, Dilan A. Patel, Kiran Vij, Feng Gao, Victoria Smith, Tae H Han, John F. Dipersio

2020-Current year OA Pubs

Off-the-shelf immunotherapeutics that suppress tumor growth and provide durable protection against relapse could enhance cancer treatment. We report preclinical studies on a CD33 x CD3 bivalent bispecific diabody, AMV564, that not only suppresses tumor growth, but also facilitates memory responses in a mouse model of acute myelogenous leukemia (AML). Mechanistically, a single 5-day treatment with AMV564 seems to reduce tumor burden by redirection of T cells, providing a time window for allogeneic or other T cells that innately recognize tumor antigens to become activated and proliferate. When the concentration of bispecific becomes negligible, the effector: target ratio has also shifted, …

Buffered Lugol's Iodine Preserves Dna Fragment Lengths, P M Gignac, D Valdez, A C Morhardt, L M Lynch

2020-Current year OA Pubs

Museum collections play a pivotal role in the advancement of biological science by preserving phenotypic and genotypic history and variation. Recently, contrast-enhanced X-ray computed tomography (CT) has aided these advances by allowing improved visualization of internal soft tissues. However, vouchered specimens could be at risk if staining techniques are destructive. For instance, the pH of unbuffered Lugol's iodine (I

From A Role-Playing Game (Rpg) To A Brand: Strategic Implications From An Over-Two-Decade Case, Cheuk Hang Au, Chih Yuan Chou, Kevin K.W. Ho

Research outputs 2022 to 2026

By offering a delightful experience, role-playing games (RPGs) have attracted many players. However, their development has been challenged by issues such as the emergence of online/mobile games and the proliferation of pirate copies. Therefore, RPG developers should consider different strategies to sustain the RPG series. We conducted a case study on “Chinese Paladin,” an RPG series that has been successful for over two decades in the Greater China region. We identified four strategies for an RPG to sustain itself and develop into a brand, namely (1) timeline-moving strategy, (2) crossover strategy, (3) sublimation strategy, and (4) cross-media strategy. We also …

Higher Plant-Derived Nitrate Intake Is Associated With Lower Odds Of Frailty In A Cross-Sectional Study Of Community-Dwelling Older Women, Eleanor Hayes, Elsa Dent, Oliver M. Shannon, Lie Zhou Zhong, Trent Bozanich, Lauren C. Blekkenhorst, Kun Zhu, Catherine P. Bondonno, Mario Siervo, Emiel O. Hoogendijk, Jonathan M. Hodgson, Richard L. Prince, Joshua R. Lewis, Marc Sim

Research outputs 2022 to 2026

Purpose: Dietary nitrate intake is inversely related to numerous contributors towards frailty, including cardiovascular disease and poor physical function. Whether these findings extend to frailty remain unknown. We investigated if habitual nitrate intake, derived from plants or animal-based foods, was cross-sectionally associated with frailty in women. Methods: Community-dwelling older Australian women (n = 1390, mean age 75.1 ± 2.7 years) completed a validated semi-quantitative food frequency questionnaire (FFQ). Nitrate concentrations in food were obtained from international nitrate databases. We adopted the Rockwood frailty index (FI) of cumulative deficits comprising 33 variables across multiple health domains (scored 0 to 1), which …

Learning Collective Care To Support Young Climate Justice Advocates, Kylie Wrigley, Georgia Beardman, Jaime Yallup Farrant, Naomi Joy Godden, Caleb Faulkner Hill, Emma Heyink, Eva Carot Collins, Hannah Davies, Shelby Robinson

Research outputs 2022 to 2026

Young people mitigate their climate distress, develop their efficacy, and contribute to the effectiveness of climate movements through activism. However, they are often excluded from adult-led climate movements and exposed to a number of risks when they do participate. In this context, this participatory action research study draws on multiple care theories to offer collective care praxis through which adults and young people might co-create more care-full and safe climate justice movements capable of supporting, sustaining, and sharing power with young people. The study examines how 13 young and three adult co-researchers learned about and applied collective care through a …

Mathematical Connections In Preservice Secondary Mathematics Teachers’ Solution Strategies To Algebra Problems, Vesife Hatisaru, Kaye Stacey, Jon R. Star

Research outputs 2022 to 2026

This study investigated the mathematical connections found in the solutions provided by 22 preservice secondary mathematics teachers to a set of algebra problems. Interest in, and research on, mathematical connections has gained prominence of the past decade. Here, we use the Extended Theory of Mathematical Connections or ETMC to explore the types of connections that this framework does and does not capture in the preservice teachers’ solutions. The ETMC surfaced four types of mathematical connections across four problems: ‘different representations’, ‘procedural’, ‘part-whole’ and ‘meaning’. The other types of connections defined in ETMC such as ‘reversibility’ or ‘feature’ were not found …

The Experience Of Occupational Therapists And Physiotherapists Using A Prototype, Evidence-Informed Online Knowledge Translation Resource To Learn About Patient-Defined, Personally-Meaningful Chronic Pain Rehabilitation, Katrina J. Liddiard, Annette J. Raynor, Cary A. Brown

Research outputs 2022 to 2026

Purpose: Chronic pain is a complex biopsychosocial experience, and rehabilitation helps people to manage pain, and restore valued life roles. Evidence suggests that more positive outcomes occur when clients perceive their rehabilitation to be meaningful. People with chronic pain describe rehabilitation as personally-meaningful when they develop a genuine connection with a credible therapist who they see as a guiding partner, and when rehabilitation holds personal value, is self-defined, and relevant to their sense of self-identity. This paper presents a qualitative study of therapists’ experience using an e-learning package on patient-defined, personally-meaningful rehabilitation. Methods: A qualitative descriptive design was used to …

Twin Scholarships Of Glycomedicine And Precision Medicine In Times Of Single-Cell Multiomics, Seungyoul Oh, Weijie Cao, Manshu Song

Research outputs 2022 to 2026

Systems biology and multiomics research expand the prospects of planetary health innovations. In this context, this mini-review unpacks the twin scholarships of glycomedicine and precision medicine in the current era of single-cell multiomics. A significant growth in glycan research has been observed over the past decade, unveiling and establishing co- and post-translational modifications as dynamic indicators of both pathological and physiological conditions. Systems biology technologies have enabled large-scale and high-throughput glycoprofiling and access to data-intensive biological repositories for global research. These advancements have established glycans as a pivotal third code of life, alongside nucleic acids and amino acids. However, challenges …

Enhancing Breast Cancer Treatment: Mesoporous Dopamine Nanoparticles In Synergy With Chrysin For Photothermal Therapy, Jing Zhu, Heng Zhang, Haomiao Lan, Bing Bi, Xianfeng Peng, Dandan Li, Haili Wang, Ke Zhu, Fuqiang Shao, Minggang Yin

Research outputs 2022 to 2026

Introduction: Breast cancer is one of the most prevalent cancers, primarily affecting women. Among its subtypes, estrogen receptor-positive (ER+) breast cancer is particularly common. Inhibiting estrogen's effects is crucial for treating ER+ breast cancer, but current therapies often have significant side effects and limitations. Chrysin, a natural flavonoid, has shown potential in reducing estrogen receptor expression, but its poor water solubility hampers clinical application. This study explores the use of mesoporous dopamine nanoparticles (mPDA) to enhance the delivery and efficacy of Chrysin, combined with photothermal therapy (PTT), for breast cancer treatment. Methods: Chrysin-loaded mPDA nanoparticles (Chrysin@mPDA) were synthesized and characterized …

Psychedelic Trip Sitting, Dosages And Intensities: Supplementing Clinical Studies With Anecdotal Reports, Liam B. Engel, Sascha Thal, Stephen J. Bright, Mitchell Low

Research outputs 2022 to 2026

Background and aims: Online communities provide insights into psychedelic consumption, assisting in identification of trends, informing both harm reduction provisions and clinical research. This study extracts forum data on psychedelic substances, dosages, and administration routes, categorising and analysing self-reported consumption to inform care services and guide clinical work with psychedelics. Methods: Posts (n = 660) from online psychedelic forums (The Shroomery and DMT Nexus) on 'trip sitting' were analysed. Using a Delphi-style expert panel review facilitated by LE, we created drug weight and intensity charts (threshold, light, common, strong and heavy dosages) for psychedelics discussed in these posts. The psychedelic …