Digital Commons Network^™

A Novel Protozoa Parasite-Derived Protein Adjuvant Is Effective In Immunization With Cancer Cells To Activate The Cancer-Specific Protective Immunity And Inhibit The Cancer Growth In A Murine Model Of Colorectal Cancer, Rajesh Mani, Chloe G. Martin, Kanal E. Balu, Qingding Wang, Piotr G. Rychahou, Tadahide Izumi, B. Mark Evers, Yasuhiro Suzuki

Markey Cancer Center Faculty Publications

Cancer-specific CD8+ cytotoxic T cells play important roles in preventing cancer growth, and IFN-γ, in addition to IL-12 and type I interferon, is critical for activating CD8+ cytotoxic T cells. We recently identified the capability of the amino-terminus region of dense granule protein 6 (GRA6Nt) of Toxoplasma gondii, an intracellular protozoan parasite, to activate IFN-γ production of microglia, a tissue-resident macrophage population. Therefore, in the present study, we examined whether recombinant GRA6Nt protein (rGRA6Nt) functions as an effective adjuvant to potently activate cancer-specific protective immunity using a murine model of MC38 colorectal cancer (CRC). When mice were immunized with non-replicable …

Retinoic Acid-Mediated Inhibition Of Mouse Coronavirus Replication Is Dependent On Irf3 And Camkk, Justin H. Franco, Ryan A. Harris, William G. Ryan, Roger Travis Taylor, Robert E. Mccullumsmith, Saurabh Chattopadhyay, Zhixing K. Pan

Markey Cancer Center Faculty Publications

The ongoing COVID-19 pandemic has revealed the shortfalls in our understanding of how to treat coronavirus infections. With almost 7 million case fatalities of COVID-19 globally, the catalog of FDA-approved antiviral therapeutics is limited compared to other medications, such as antibiotics. All-trans retinoic acid (RA), or activated vitamin A, has been studied as a potential therapeutic against coronavirus infection because of its antiviral properties. Due to its impact on different signaling pathways, RA’s mechanism of action during coronavirus infection has not been thoroughly described. To determine RA’s mechanism of action, we examined its effect against a mouse coronavirus, mouse hepatitis …

Foxs1 Is Increased In Liver Fibrosis And Regulates Tgfβ Responsiveness And Proliferation Pathways In Human Hepatic Stellate Cells, Evelyn A. Bates, Zachary A. Kipp, Wang-Hsin Lee, Genesee J. Martinez, Lauren Weaver, Kathryn N. Becker, Sally Pauss, Justin F. Creeden, Garrett B. Anspach, Robert N. Helsley, Mei Xu, Maria E. C. Bruno, Marlene E. Starr, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

Liver fibrosis commences with liver injury stimulating transforming growth factor beta (TGFβ) activation of hepatic stellate cells (HSCs), causing scarring and irreversible damage. TGFβ induces expression of the transcription factor Forkhead box S1 (FOXS1) in hepatocytes and may have a role in the pathogenesis of hepatocellular carcinoma (HCC). To date, no studies have determined how it affects HSCs. We analyzed human livers with cirrhosis, HCC, and a murine fibrosis model and found that FOXS1 expression is significantly higher in fibrotic livers but not in HCC. Next, we treated human LX2 HSC cells with TGFβ to activate fibrotic pathways, and FOXS1 …

Mir-27a-3p Regulates Intestinal Cell Proliferation And Differentiation Through Wnt/Β-Catenin Signalling, Chang Li, Yuning Zhou, Yinping Jiang, Zhijie Yin, Heidi L. Weiss, Qingding Wang, B. Mark Evers

Markey Cancer Center Faculty Publications

Intestinal stem cells differentiate into absorptive enterocytes, characterised by increased brush border enzymes such as intestinal alkaline phosphatase (IAP), making up the majority (95%) of the terminally differentiated cells in the villus. Loss of integ- rity of the intestinal epithelium plays a key role in inflammatory diseases and gastro- intestinal infection. Here, we show that the intestinal microRNA (miR)-27a-3p is an important regulator of intestinal epithelial cell proliferation and enterocyte differenti- ation. Repression of endogenous miR-27a-3p leads to increased enterocyte differen- tiation and decreased intestinal epithelial cell proliferation in mouse and human small intestinal organoids. Mechanistically, miR-27a-3p regulates intestinal cell …

Interactions Between Multiple Stressors, Nanomaterials And A Pathogen, In Caenorhabditis Elegans, Jarad Cochran

Theses and Dissertations--Plant and Soil Sciences

Metal and metal oxide nanoparticles (NP), which have received interest for use in agriculture, show in vitro and in vivo toxicity at relatively low concentrations. Silver nanoparticles (Ag NP) and zinc oxide nanoparticles (ZnO NP) cause adverse effects in model soil organisms under environmentally relevant conditions. In most toxicity assessments of nanomaterials, model organisms are exposed to a single stressor. Though single stressor experiments are essential to understanding the fate and effects of released NP, in nature, organisms are affected by multiple stressors, including pathogens, which might exacerbate or mitigate negative effects of NP exposure. There is limited research on …

Local Government Regulation Of Ccs, Keith B. Hall

Journal Articles

Carbon capture and storage is being used in an increasing number of locations in the United States as a tool to address climate change, and many more projects are in the planning stages. Although government, industry, and some prominent environmental organizations support the use of CCS, some environmental organizations and many citizens who live in the vicinity of proposed CCS projects oppose CCS. This has prompted several local governments to enact ordinances that attempt to prohibit or regulate CCS. These ordinances take many forms-moratoria, zoning restrictions and setbacks, effective bans, outright bans, construction and operational restrictions, and various other types …

Use Of Thermal Microhabitats By Herpetofauna In A Fragmented Rainforest Landscape In Colombia, Catalina Valderrama

Masters Theses

No abstract provided.

Influences Of Professors' Personality Traits, Teaching Styles, And Generational Affiliation On Technology Acceptance: A Mixed-Methods Exploration Of Blended Learning Readiness, Joey K. Freeman

Education Dissertations

The abrupt transition to emergency remote teaching during the COVID-19 pandemic offered minimal time to prepare, resulting in faculty reliance on prior pedagogical practices in the shift to an online instructional environment. Although the pandemic altered the learning environment in the short-term, it also offered a glimpse of how higher education institutions (HEIs) could transform their learning environments in the long-term. Though a body of literature exists on factors that influence technology acceptance, a limited number of studies have explored personality traits, teaching styles, and generational affiliation. An adapted Technology Acceptance Model considered these variables as antecedents to perceptions, attitudes, …

Blinded By The Light: Resolving The Conflict Between Satellite Megaconstellations And Astronomy, David A. Koplow

Vanderbilt Journal of Transnational Law

The sudden emergence of large constellations of small satellites in low altitude orbits represents one of the most dramatic contemporary innovations in outer space. Promising low-cost, low-latency global communications and spectacular capacities for remote sensing of the Earth, these satellites will soon number in the tens of thousands, sponsored by diverse corporations and countries around the world. But this proliferation of spacecraft comes at a steep cost in unavoidable interference with ground-based astronomy: as the satellites overfly the observatories, they block the views of remote objects and phenomena, leaving obliterating white streaks on the collected imagery, and obscuring access to …

Strafford County 2023 Annual Report Of The Commissioners, Treasurer, Other County Officers, And The Strafford County Delegation For The Year Ending December 31, 2023, Strafford County Representatives

Strafford County, NH Annual Reports

This is an annual report containing vital statistics for a county in the state of New Hampshire.

Diverse Minds, Shared Planet: Designing, Assessing, And Adapting Curricula To Engage Diverse Learners With Their Environment And Inspire Positive Change, Mallory Rose Lampe

Graduate Student Portfolios, Professional Papers, and Capstone Projects

The pieces that compose this portfolio together explore how environmental education can connect diverse learners to their environment through the integration of accessible curricula to inspire stewardship and action to take care of the planet we share.

Vertical Farming: A Key To Sustainable Compact Cities, Mohab Taher Abdelfatah, Sahar Mahmoud El-Arnaouty, Akram Abdelhakim Zayan

Mansoura Engineering Journal

Urbanization continues to surge, with projections indicating that by 2050, around 70 percent of the global population will reside in urban areas. In response, urban planners are exploring novel urban paradigms to ensure food security through urban agriculture, a cornerstone of sustainable development encompassing environmental, economic, and social dimensions. This study addresses a critical research gap within urban agriculture, conducting a comparative analysis between horizontal and vertical farming methodologies within urban contexts. By categorizing various typologies of vertical farming, from container farms to mixed-use structures, this research highlights their distinct contributions to sustainable urban development. Emphasis is placed on vertical …

Acute Postoperative Pain And Dorsal Root Ganglia Transcriptomic Signatures Following Total Knee Arthroplasty (Tka) In Rats: An Experimental Study, Justice Achonu, David E. Komatsu, Sardar Mz Uddin, Chris Gordob, Martha Kanjiya, Diane Bogdan, Adriana Dibua, Hira Iftikhar, Amanda Ackermann, Rohan J. Shah, Jason Shieh, Agnieszka B. Bialkowska, Martin Kaczocha

Stony Brook Orthopaedic and Musculoskeletal Journal

Total knee arthroplasty (TKA) is the final treatment option for patients with advanced knee osteoarthritis (OA). Unfortunately, TKA surgery is accompanied by acute postoperative pain that is more severe than arthroplasty performed in other joints. Elucidating the molecular mechanisms specific to post-TKA pain necessitates an animal model that replicates clinical TKA procedures, induces acute postoperative pain, and leads to complete functional recovery. Here, we present a new preclinical TKA model in rats and report on functional and behavioral outcomes indicative of pain, analgesic efficacy, serum cytokine levels, and dorsal root ganglia (DRG) transcriptomes during the acute postoperative period. Following TKA, …

The Impact Of School Nurses: A Program Evaluation Of K-12 School Nursing In The Commonwealth Of Virginia, Clarence D. Collins, Edwina Mcneill-Simaan, Lyons Hardy, Amber M. Butler

Doctor of Education Capstones

Since the early 1900s, the provision of school nursing and school health services in the K-12 educational setting has been an important component of supporting student academic success, reducing student absenteeism, and promoting positive health outcomes in the United States. The Commonwealth of Virginia was historically one of the first states in the U.S. to employ school nurses. K-12 schools in Virginia have continued to support the practice of school nursing for over 100 years; however, the Virginia state legislature has not formalized a requirement for school nurses in the state code. The Virginia Department of Education (VDOE) is responsible …

Tension Between Construction And Conservation : Dutch Gap Conservation Area Park Access Design, Colleen Campbell

Master of Urban and Regional Planning Capstone Projects

The goal of this plan is to create a site-specific design as well as investigate the best practices to use to aid in mending the tension between conservation and construction especially in conservation areas. This comes in response to legislation passed by the Virginia General Assembly in 2019. Using literature, expert surveys, and public input a design will be created to aid the public in accessing the Dutch Gap Conservation Area. Many challenges are present in conservation areas and this plan will help to address which challenges are most important as well as the best way to mitigate these challenges. …

A Business Model For Communication Design Enterprises, Con Kennedy

Articles

Several international reports and studies indicate that the Communication Design sector has a skill deficit in entrepreneurship and business management, and a greater understanding of these topics is needed. Some authors advocate applying existing business models for design enterprises. However, the skill deficit prevails. It is worth noting that these reports discuss the entrepreneurship deficit in general terms without an explanation of the causes of this deficit or how to remedy the issue. Therefore, this paper explores the development of a Business Model specific for Design Entrepreneurs for deployment in Communication Design enterprises.A theoretical framework for a specific business model …

Building A Reciprocal Partnership: A Case Study Of The Relationship Between The University Of South Dakota And Its Privately-Owned Sorority And Fraternity Housing, Kelli L. Susemihl

Dissertations and Theses

Higher education institutions with Greek systems have different models of sorority and fraternity housing. There are two primary models incorporated into this study; some institutions have Greek housing incorporated into their on-campus residence halls, while other Greek housing is privately-owned by local House Corporation Boards or by the chapter’s national headquarters. The University of South Dakota follows the latter model, with each of their eleven chapters having a privately-owned chapter house. Research shows that with privately-owned Greek housing, there is an increased risk of substance abuse and sexual activity, while there are benefits of increased student involvement and leadership development. …

When Can An Agreement On Environmental Policies Comply With U.S. Antitrust Laws?, Nathan Mendelsohn

Emory Business Law Review

No abstract provided.

It’S Not Personal, It’S Strictly Business: The Need To Amend The Federal Rules Of Evidence To Permit Live Corporate Designee Testimony At Trial, Veronica J. Finkelstein

Emory Business Law Review

No abstract provided.

Troubling Waters: Anthropocene Marine Gothic In The 19th-Century Anglo-American Fiction, Phoenix Guqing Wang

Comparative Literature M.A. Essays

The 19th century witnessed intensifying marine ecological disruptions by colonial, industrial, and imperial activities. Meanwhile, current scholarship acknowledges the significance of the 19th century in tracing the emergence of the “Anthropocene” – a loose shorthand for ecological damages that are planetary in scale. This thesis aims to analyze how gothic imagination of the marine space unravels the linkages between the two temporally intersecting phenomena. Drawing on ocean studies, ecocriticism, new materialism, and gothic studies, I theorize what I call “Anthropocene marine Gothic discourse” to understand how 19th-century Anglo-American writers attend to the marine space to grapple with planetary ecological concerns …

Locating And Assessing Oregon Trail Ruts With Aerial Stereophotographs, Satellite Imagery And Lidar, P.R. Hanson, Robert Matthew Joeckel, L. M. Howard

Conservation and Survey Division: Faculty and Staff Publications

No abstract provided.

Integrated Geochronological And Chemostratigraphic Study Of Middle Miocene Strata (Ogallala Group) At The Eastern Margin Of The North American Great Plains, J. J. Smith, Robert Matthew Joeckel, H. L. Field, G. A. Ludvigson, A. Moller, A. Layzell, S. T. Tucker

Conservation and Survey Division: Faculty and Staff Publications

No abstract provided.

From Mind Only To Buddha Only: Shakya Chokden’S Interpretation Of Tathāgatagarbha In Tantric Philosophy And Practice, Yaroslav Komarovski

Department of Classics and Religious Studies: Faculty Publications

Shakya Chokden’s approach consists of reducing all phenomena to mind and its appearances, and then negating dualistic minds as illusory and nonexistent and establishing the non-dual jñāna transcending them as the only phenomenon existent in reality—the primordial tathāgatagarbha with all buddha-qualities complete. For him, this interpretation of tathāgatagarbha is indispensable for tantric contemplative practice and for explaining how and why that practice works. The initial identification of tathāgatagarbha as the primordially existent jñāna endowed with buddha-qualities serves as the foundation for conceptually envisioning deities of the ultimate body-maṇḍala and eventually, when enough clarity is achieved, directly perceiving them by jñāna …

The Effectiveness Of Dental Pulp Stem Cells-Derived Exosomes For Direct Pulp Capping: In Vitro Study, Dina A. Hammouda, Alaa M. Mansour, Mohammed E. Grawish, Ahmed R. Zaher

Mansoura Journal of Dentistry

Objective: This in vitro investigation's goal was to ascertain how well dental pulp stem cell exosomes (DPSC-exo) modulate endogenous dental pulp stem cells (DPSCs) and direct their navigation toward mineralized tissue regeneration, either alone or in conjunction with mineral trioxide aggregate (MTA). Materials and methods: For this investigation, DPSCs were harvested using rat incisors. To extract exosomes from DPSCs, an ultracentrifugation method was employed. Flow cytometry was used for the characterization of DPSCs-exo, and transmission electron microscopy (TEM) was used to measure their size. DPSCs were incubated in standard culture media and split up into three groups. The first one …

Biocompatibility Evaluation Of Edta And Chitosan Nanoparticles Gel Forms: In Vivo Histopathological Study, Alaa H. Youssef, Doaa A. Labah, Abeer A. Elgendy, Amany E. Badr

Mansoura Journal of Dentistry

Objectives: The purpose of this study was to evaluate and compare biocompatibility of gel form chelating agents (EDTA and chitosan NPS) after implantation of polyethylene tubes in rats. Materials and methods: Twenty albino rats were included in this study. Three horizontal incisions were performed on dorsal surface of each rat to receive three polyethylene tubes as subcutaneous implants; two of them filled with tested materials and one empty tube served as control. After 2 and 4 weeks of subcutaneous implantation, 10 rats were sacrificed and implanted polyethylene tubes with surrounding tissues were dissected with 1 cm safety margin. The specimens …

The Impact Of Social Media On Charitable Giving For Nonprofit Organization, Namchul Shin

Journal of International Technology and Information Management

Research has extensively studied nonprofit organizations’ use of social media for communications and interactions with supporters. However, there has been limited research examining the impact of social media on charitable giving. This research attempts to address the gap by empirically examining the relationship between the use of social media and charitable giving for nonprofit organizations. We employ a data set of the Nonprofit Times’ top 100 nonprofits ranked by total revenue for the empirical analysis. As measures for social media traction, i.e., how extensively nonprofits draw supporters on their social media sites, we use Facebook Likes, Twitter Followers, and Instagram …

Inaugural Future Of Bse Days: Workshop Report, Mark Stone

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

The inaugural Future of BSE Days (FBD) event, held on May 2-3, 2024, brought together the Biological Systems Engineering (BSE) community to discuss and shape the future of work and learning within the field. The event was designed to foster collaboration between faculty, students, industry leaders, and alumni, with the goal of reimagining the department’s educational approach, enhancing industry partnerships, and preparing students for the challenges of tomorrow. Over the course of two days, participants engaged in insightful discussions, workshops, and presentations that highlighted both the strengths of the department and opportunities for growth.

The workshop resulted in several key …

Adding Value To Crop Production Systems By Integrating Forage Cover Crop Grazing, Robert B. Mitchell, Daren D. Redfearn, Kenneth P. Vogel, Terry J. Klopfenstein, Galen Erickson, P. Steven Baenziger, Bruce E. Anderson, Mary E. Drewnoski, Jay Parsons, Steven D. Masterson, Marty R. Schmer, Virginia L. Jin

Department of Agronomy and Horticulture: Faculty Publications

In addition to their value as cereal grains, wheat (Triticum aestivum L.) and triticale (× Triticosecale Wittmack) are important cool-season annual forages and cover crops. Yearling steer (Bos taurus) performance was compared in the spring following autumn establishment as for age cover crops after soybean [Glycine max (L.) Merr.] grain harvest. Replicated pastures (0.4 ha) were no-till seeded in three consecutive years into soybean stubble in autumn, fertilized, and grazed the following spring near Ithaca, Nebraska, USA. Each pasture (n = 3) was continuously stocked in spring with four yearling steers (380 ± 38 kg) for …

Classification Of Sow Postures Using Convolutional Neural Network And Depth Images, Md Towfiqur Rahman, Tami M. Brown-Brandl, Gary A. Rohrer, Sudhendu R. Sharma, Yeyin Shi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The United States swine industry reports an average preweaning mortality of approximately 16% where approximately 6% of them are attributed to piglets overlayed by sows. Detecting postural transitions and estimating sows’ time budgets for different postures are valuable information for breeders and engineering design of farrowing facilities to eventually reduce piglet death. Computer vision tools can help monitor changes in animal posture accurately and efficiently. To create a more robust system and eliminate varying lighting issues within a day including daytime/ nighttime differences, there is an advantage to using depth cameras over digital cameras. In this study, a computer vision …

Biogeographic Affiliation And Centers Of Richness As Predictors Of Elevational Range-Size Patterns For Malesian Flora, Melissa Whitman, Sabrina E. Russo

School of Biological Sciences: Faculty Publications

Our goal was to interrogate the idea that “mountain passes are higher in the tropics” by investigating ecological and biogeographic drivers of elevational range-sizes patterns among equatorial flora. We used herbarium records for 60 species-rich plant families, representing 18 535 species total, to estimate distributions over a 4500 m elevational gradient. For each family, we estimated the change in average range-sizes with increasing elevation (i.e. Rapoport's rule, abbreviated as ERR) and quantified 15 metrics of familial richness distribution, evolutionary age, and biogeographic affiliation. We visualized covariation across families using phylogenetic principal components analysis (pPCA). We then evaluated how family-level ERR …