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Chemoreflex Sensitization Occurs In Both Male And Female Rats During Recovery From Acute Lung Injury, Kajal Kamra, Irving H. Zucker, Harold D. Schultz, Han-Jun Wang

Journal Articles: Cellular & Integrative Physiology

Introduction: Sex-specific patterns in respiratory conditions, such as asthma, COPD, cystic fibrosis, obstructive sleep apnea, and idiopathic pulmonary fibrosis, have been previously documented. Animal models of acute lung injury (ALI) have offered insights into sex differences, with male mice exhibiting distinct lung edema and vascular leakage compared to female mice. Our lab has provided evidence that the chemoreflex is sensitized in male rats during the recovery from bleomycin-induced ALI, but whether sex-based chemoreflex changes occur post-ALI is not known. To bridge this gap, the current study employed the bleomycin-induced ALI animal model to investigate sex-based differences in chemoreflex activation during …

Targeted Insertion Of Conditional Expression Cassettes Into The Mouse Genome Using The Modified I-Pitt, Hiromi Miura, Ayaka Nakamura, Aki Kurosaki, Ai Kotani, Masaru Motojima, Keiko Tanaka, Shigeru Kakuta, Sanae Ogiwara, Yuhsuke Ohmi, Hirotaka Komaba, Samantha L. P. Schilit, Cynthia C. Morton, Channabasavaiah B. Gurumurthy, Masato Ohtsuka

Journal Articles: Genetics, Cell Biology & Anatomy

BACKGROUND: Transgenic (Tg) mice are widely used in biomedical research, and they are typically generated by injecting transgenic DNA cassettes into pronuclei of one-cell stage zygotes. Such animals often show unreliable expression of the transgenic DNA, one of the major reasons for which is random insertion of the transgenes. We previously developed a method called "pronuclear injection-based targeted transgenesis" (PITT), in which DNA constructs are directed to insert at pre-designated genomic loci. PITT was achieved by pre-installing so called landing pad sequences (such as heterotypic LoxP sites or attP sites) to create seed mice and then injecting Cre recombinase or …

Thrive: Success Strategies For The Modern-Day Faculty Member, Elizabeth L. Beam, Betsy J. Becker, Jeana Benton, Megan Berger, Garima Bhandari, Eric Bloomquist, Brian P. Boerner, Kathleen Brandert, Emily Brandt, Liliana Bronner, Russell J. Buzalko, Kim Castellano, Creative Coaching, Teresa Cochran, Giovanni Consolino, Beth Culross, Tanya M. Custer, Precious Davis, Dele Davies, Holly Dingman, Jayme Dowdall, Steven Ebers, Mary Edgerton, April Elker, Bryant England, Sandra Friimpong, Kellie Gossman, Julie Gregg, Dianna Hemsath, Karen Honeycutt, Michelle Howell, Grace C. Johnson, Heidi Keeler, Victoria Kennel, Birgit Khandalavala, Jess King, Jessica Koran-Scholl, Gail Kotulak, Nora Kovar, Michael Kozak, Darby Kurtz, Crystal Krause, Trek Langenhan, Jennifer L. Larsen, Kristan Lester, Bethany Lowndes, Linda M. Love, Riley D. Machal, Alberto Marcelin, Jasmine R. Marcelin, Jessica Maxwell, Sarah B. Mcbrien, Analisa Mcmillan, Carrie Meyer, Kyle Meyer, Jane L. Meza, Kim Michael, Luann Mill, Peggy Moore, Judy Neville, Dana Raml, Priscila Rodrigues Armijo, Cory Rohlfsen, Khalid Sahak, Lepaine Sharp-Mchenry, Kari Simonsen, Nicole M. Sleddens, Zachary P. Slocum, Christopher J. Smith, Michael Smith, Matt Sorensen, G. Talmon, Erin J. Torell, Kathleen G. Volkman, Jana L. Wardian, Steven P. Wengel, Debra Wekesa, Tessa M. Wells, Maggie Winnicki, Rowen K. Zetterman, Andrea Zimmer, Kenneth A. Zoucha

Books: Faculty Development

The THRIVE collection is intended to help faculty thrive in their roles as educators, scholars, researchers, and clinicians. Each section contains a variety of thought-provoking topics that are designed to be easily digested, guide personal reflection, and put into action. Please use the THRIVE collection to help:

• Individuals study topics on their own, whenever and wherever they want
• Peer-mentoring or other learning communities study topics in small groups
• Leaders and planners strategically insert faculty development into existing meetings
• Faculty identify campus experts for additional learning, grand rounds, etc.

If you have questions or want additional information on a topic, simply …

Declaring And Terminating Public Health Emergencies: Performative Utterances That Can Change The World, Christine N. Coughlin, Ana S. Iltis

Maryland Law Review

No abstract provided.

Editorial: Intersection Of Diet, Intestinal Microbiota, And Their Metabolites On Cancer Prevention, Nancy D. Turner, Tiffany L. Weir, Jacques Izard

Journal Articles: Internal Medicine

No abstract provided.

The Ambivalent Logics Of Business Representation In International Organizations, Melissa (M.J.) Durkee

Scholarship@WashULaw

The United Nations and its bodies have 'opened up' to a broad range of non-state actors over the last three decades, including for-profit actors and their representatives. The shift is reflected in the UN's sustainable development goals and the Global Compact, emphasizing public-private partnerships; in greater participation of corporations at treaty conferences; in trade group roles as observers at organizations; and in multi-stakeholder projects. Yet international organizations have generally not developed robust responses to legitimacy concerns about businesses becoming closely involved in lawmaking and governance projects. These concerns focus on interest group capture, entrenchment of western economic elites, creeping privatization, …

Interplay Of Antibody Responses To Closely Related Viruses And Autoimmunity, Chun-Yang Lin

Theses and Dissertations (ETD)

Antibodies produced by B cells are crucial for eliminating infectious pathogens and preventing secondary infection. However, antibodies may also bind to self-antigens and contribute to autoimmune disease. While pathogen infections have been associated with development of autoimmune diseases, the precise contributions of viral infections to autoimmunity remain elusive. The presence of autoreactive antibodies is associated with an increased risk of developing autoimmune disease. Thus, analyzing the impact of viral infection and vaccination on the development of autoreactive antibodies will provide essential insight into mechanisms that contribute to autoimmune disease.

Examining How Non-Antifungal Medications Impact Host-Fungal Interactions, Parker Ryan Reitler

Theses and Dissertations (ETD)

Invasive fungal infections (IFIs) are estimated to kill 1.5 million people each year with mortality rates remaining high (around 30-70%), despite the availability of three major classes of antifungal drugs. Furthermore, millions more suffer debilitating infections of the mucosal membranes or destructive subcutaneous mycoses. One of the most common IFIs is invasive candidiasis, mostly commonly caused by Candida albicans infections, with mortality rates of approximately 30%. In addition, the population of susceptible individuals and the incidences of both mucosal and systemic mycoses continue to rise due to the widespread use of broad-spectrum antibacterial agents, cancer chemotherapies, and the immune suppressive …

Molecular Determinants Of Trp Channel Gating, Briar J. Bell

Theses and Dissertations (ETD)

Transient Receptor Potential (TRP) ion channels are a family of proteins that contribute to a myriad of physiological functions. They have been extensively studied since their discovery 35 years ago; however, there are still many questions regarding the mechanisms by which they function. This family of proteins is characterized by a generally shared structure: an intracellular N terminus featuring ankyrin repeats, six transmembrane helices including the pore domain, a re-entrant loop and TRP helix, and an intracellular C terminus with varying structure. Each of these regions has been implicated in the regular function of TRPs, while mutations that alter trafficking …

Anatomical And Physiological Characterization Of Striatal Activation Of The Cerebral Cortex, Yuhan Wang

Theses and Dissertations (ETD)

Purpose. The striatum receives excitatory glutamatergic input from nearly all parts of the cerebral cortex, and its output is delivered by direct pathway medium spiny neurons (dMSNs) expressing D1 type dopamine (DA) receptors (D1Rs) and indirect pathway MSNs (iMSNs) expressing D2 DA receptors (D2Rs). Loss of DA results in the imbalance between the activities of dMSNs and iMSNs in Parkinson’s disease (PD), leading to ab-normal interactions between the cerebral cortex and striatum. Thus, understanding the inter-actions between the cortex and striatum is important. The monosynaptic corticostriatal cir-cuit has been extensively studied. In contrast, due to the poly-synaptic nature of the …

Characterizing The Cellular Response To Astrovirus Infection In The Central Nervous System, Amy E. Davis

Theses and Dissertations (ETD)

Central nervous system (CNS) diseases caused by infectious agents pose significant health challenges, often leading to severe neurological dysfunction with high morbidity and mortality rates. Astroviruses (AstVs), traditionally known for causing gastroenteritis, have recently been identified as neuroinvasive pathogens capable of inducing neurological diseases. This study explores the cellular and molecular mechanisms by which AstV, specifically non-classical VA1 and classical HAstV1 genotypes, induce CNS pathology in human neurons and astrocytes. Utilizing primary human neurons and astrocytes from various brain regions, this research investigates the cytotoxic effects, apoptotic pathways, and inflammatory responses triggered by these viruses. This study reveals that VA1 …

Improving Health Outcomes In Children And Adolescents With Asthma, Elizabeth Kate Coffey Moore

Theses and Dissertations (ETD)

Asthma is among the most prevalent chronic illnesses found in the United States, affecting millions of people under the age of 18 each year. The disease burden for affected children, adolescents, their families, and the healthcare system is immense. Finding ways to mitigate its impact is critical to improving the lives of affected children, adolescents, and their families. The three chapters included in this dissertation aimed to document the asthma triggers that children and adolescents are exposed to and strategies for reducing them and to demonstrate using Imogene King’s conceptual framework to guide nursing practice to care for and nursing …

La Négociation De L’Altérité Aux Marges : L’Agentivité Des Aventuriers Dans Les Sauteurs Et The Land Between, Destiny Rosulme , '24

Senior Theses, Projects, and Awards

No abstract provided.

Imaginer La Vie De La Fin De L'Anthropocène Dans La Mort De La Terre, Caroline Lucas , '24

Senior Theses, Projects, and Awards

No abstract provided.

Speaking Back To Sexual Privacy Invasions, Brenda Dvoskin

Scholarship@WashULaw

Many big players in the internet ecosystem do not like hosting sexual expression. They often justify these bans as a protection of sexual privacy. For example, Meta states that it removes sexual imagery to prevent the nonconsensual distribution of sexual images. In response, this Article argues that banning digital sexual expression is counterproductive if the aim is to alleviate the harms inflicted by sexual privacy losses.

Contemporary sexual privacy theory, however, lacks analytical tools to explain why nudity bans harm the interests they intend to protect. This Article aims at building those tools. The main contribution is an invitation to …

Provisioning Digital Tools And Systems For Government Use, Brenda Dvoskin, Julie E. Cohen, Paul Ohm, Meg Leta Jones, Smitha Krishna Prasad, Nina-Simone Edwards

Scholarship@WashULaw

This document is part of a larger project aimed at reinventing the administrative state for effective governance of the digital, information-driven economy. It explores how the administrative state can more effectively equip itself with digital tools and systems that align with and improve government’s ability to serve public values. Established approaches to digital provisioning fail in many important respects. Among others, they introduce thorny coordination problems while doing little to ensure design for broader public values; they cause obsolete and/or poorly conceived requirements to cascade through the development process for new tools and systems; they magnify the potential for technology-driven …

Independence Through Judicialization: The Politics Surrounding Administrative Adjudicators 1929-1949, Lawrence J. Liu

Scholarship@WashULaw

One front in today’s battle to define the scope of the administrative state concerns the authority, status, and future of its 10,000-plus administrative adjudicators. Decisions by federal courts and the executive branch to increase the dependence of administrative adjudicators on the executive have sparked strong reactions from observers, with many advocating for measures to increase adjudicator “independence.” But who should administrative adjudicators be independent of, which ought to be independent, and why? Calls for administrative adjudicator independence are not new. This Article draws on primary documents produced by private actors, congressional decisionmakers, and federal executive agents to present a political …

Evaluation Of A Novel Corn Fermented Protein Ingredient, Probiotics, And Postbiotics On Nursery Pig Growth Performance And Gut Health, Juan F. Castillo Zuniga

Electronic Theses and Dissertations

Weaning is a necessary step in pig production. After weaning, pigs are subjected to various nutritional, psychological, and environmental stresses. The weaning period is an important period for the intestinal development of the pig. During this time, digestion, immunity, metabolism, and various other aspects of the pig change rapidly. Therefore, providing high-quality feed ingredients is crucial to encourage the newly weaned pig to start consuming solid feed. Two independent studies were conducted to determine if the inclusion of: 1) corn fermented protein (CFP) or 2) probiotics or postbiotics impact nursery pigs' growth performance and gut health. The first experiment utilized …

Field Scale Evaluation Of A Flow-Through Filtration System With Mixed Media To Remove E. Coli From Stormwater, Ankur Debnath

Electronic Theses and Dissertations

The main goal of this research was to establish a field-scale stormwater filtration system which is flow-through and cost-effective in terms of construction and maintenance. The filter is constructed in a downstream drainage of a retention pond where the stormwater goes through the porous space of media so that it maintains low retention time with minimum disturbance in flow. The filter consists of two 20ft long sections with concrete channel and ripraps on the inlet & outlet side. 30% Steel chips and 70% steel slags in the size range of 6.00-12.5 mm have been used as media which has been …

The Relationship Between Soil Test Phosphorus And Soil Biology - A Natural Management Of Soil Phosphorus Through Conservation Practices, Clarence Frederick Winter

Electronic Theses and Dissertations

Phosphorus (P) management is an ongoing challenge in modern agriculture. Due to the importance of P in agriculture, P fertilizer additions are made throughout the U.S. to avoid nutrient-depleted soil. However, the application of P is problematic, as an excess of P in an agricultural system elevates the risk of environmental degradation, and current synthetic P sources are a fleeting resource. Additionally, a majority of P found in agricultural soils are insoluble, in the form of secondary and primary P minerals, making this large pool of P unavailable to plants. Fortunately, soil fungi organisms, such as arbuscular mycorrhizal fungi (AMF) …

Identification And Characterization Of Three Novel Rumen Bacterial Species In Sheep Fed A Concentrate Diet, Reid Frederick Anema

Electronic Theses and Dissertations

Sheep production in the United States is a popular venture for both small farmers and large enterprise owners, as there is a market for end products such as wool and meat. One major factor of raising sheep is nutrition as an adequate diet will allow an animal to reach its full genetic potential for growth. With that in mind, the composition of the diet must be formulated to benefit the animal. Ruminants have the ability to use a variety of plants that are often inedible for humans to create products that humans can consume. These varying feedstuffs, whether they include …

Anemia In Highly Prolific Sows: Implications On Piglet Outcomes, Farrowing Complications, And Longevity Considerations, Katlyn A. Mcclellan

Electronic Theses and Dissertations

No abstract provided.

Ecophysiology Of Bradyrhizobium Diazoefficiens Usda 110, Armaan Kaur Sandhu

Electronic Theses and Dissertations

With increasing demand for soybean, maximizing plant yield is important yiel is dependent on nitrogen availability, but nitrogen fertilizers can meet the plant's needs but have severe environmental impacts. An economically and environmentally sustainable approach is to enhance nitrogen availability through Biological Nitrogen Fixation by symbiotic rhizobia, Bradyrhizobium, an efficient nitrogen fixer for soybean. This symbiosis begins with attachment of rhizobia to legume roots. Root and rhizobia surface properties play a significant role in this attachment. Another major factor affecting root colonization is bacterial competition ability. There is a lack of knowledge on the role of bacterial surface properties, competition …

Role Of Interferon Induced Transmembrane Protein 3 (Ifitm3) And Neural Precursor Cell Expressed Developmentally Down Regulated Protein 4 (Nedd4) In Seneca Virus A (Sva) Replication, Shamiq Aftab

Electronic Theses and Dissertations

In this study, we studied the role of IFITM3 in SVA replication in vitro by silencing the endogenous IFITM3 in NCI-H1299 cells. An average of 1.94-fold increase in SVA VP2 expression and an average of 4.4-fold increase in supernatant virus titer was observed after 24h SVA isolate SD15-26 infection. We have indeed observed an increase in viral mRNA copies by an average of 5-fold in IFITM3 silenced NCI-H1299 cells at 8 hpi indicating that IFITM3 restricts SVA replication post virus attachment and entry. A positive correlation between interferon-alpha induced IFITM3 upregulation and reduced SVA replication was observed. To further confirm …

Dutchtown South Waste Services Equity, Erika Adjei, Samantha Campisi, Serena Earp, Erin Hill, Hannah Hummel, Lourdes Letelier, Joe Mantych, Sydney Rottinghaus

Sustainability Exchange Projects

No abstract provided.

Thirty-Sixth Annual Bibliography 2022 (Mike Lützeler Contemporary German Literature Collection), Walter Schlect, Kristian Svane

WashU Libraries Publications

The 36th bibliography of 850 volumes added to Washington University Libraries' Mike Lützeler Contemporary German Literature Collection. All published in 2021, these acquisitions include novels, poetry, short story collections, graphic novels, essays, autobiographical works, and literary and cultural periodicals from publishers in Germany, Austria, and Switzerland. Organized by author or editor, the bibliography includes local call numbers as well as subject and genre descriptors. This Collection serves as the research arm for the Department of Germanic Languages and Literature's Max Kade Center for Contemporary German Literature. A bibliography of items added the previous year is compiled each year …

Regulatory Landscapes: Transcriptome And Microrna Regulated Senescence And Dormancy In Grapevines And Gene Regulatory Networks In Nitrogen-Fixing Soybean Nodules, Ramandeep Kaur

Electronic Theses and Dissertations

Grapevine and soybean are two economically important crops, each with unique yet equally crucial physiological processes that profoundly influence their growth and productivity. Grapevine (Vitis sp.), a temperate fruit crop, employs dormancy to withstand winter challenges and can grow in various regions worldwide. Understanding the molecular mechanisms involved in dormancy in Vitis species, is crucial for improving grapevine selection for resilience in northern temperate regions. Dormancy induction is modulated by decreasing photoperiods in some Vitis species, enhancing their ability to prepare for winter stress conditions. Understanding the molecular underpinnings of response to photoperiod will provide potential markers for selecting genotypes …

Search Strategies For "Characterizing Metastatic Anogenital Squamous Cell Carcinoma In Immunosuppressed Patients: A Systematic Review", Sierra Thomas, Molly Antonson, Megan Wackel, Cynthia M. Schmidt, Ashley Wysong

Search Strategies: Leon S. McGoogan Health Sciences Library

No abstract provided.

Search Strategies For"Analgesic Safety And Efficacy Of Peri-Operative Posterior Cervical Muscle Plane Blocks In Elective Posterior Cervical Spine Surgery: A Systematic Review With Meta-Analyses", Luke J. Weisbrod, Omar I. Ceesay, Cynthia M. Schmidt, Daniel Surdell

Search Strategies: Leon S. McGoogan Health Sciences Library

No abstract provided.

Search Strategies For “Analgesic Safety And Efficacy Of Intrathecal Morphine In Elective Posterior Lumbar Fusion: A Systematic Review With Meta-Analyses”, Luke J. Weisbrod, Jordan M. Rasmussen, Cynthia M. Schmidt, Daniel Surdell

Search Strategies: Leon S. McGoogan Health Sciences Library

No abstract provided.