Digital Commons Network^™

Measuring Spontaneous Neurotransmission Using Genetically Encoded Fluorescent Indicators, Amelia Jane Ralowicz

Dartmouth College Ph.D Dissertations

Synaptic connections throughout the nervous system exhibit two forms of neurotransmitter release caused by synaptic vesicle fusion, evoked and spontaneous release. Despite being discovered 70 years ago, the mechanisms governing spontaneous release have remained elusive. Our understanding of spontaneous release lacks mechanistic details including discrepancies in the sources of synaptic vesicles supporting it, the source(s) of calcium supporting it, and most importantly an unclear relationship (cooperative or antagonistic) with evoked release. These gaps exist due to a lack of experimental methods specific to the study of spontaneous release at individual synapses.

This thesis addresses these gaps in two ways. Firstly, …

Identification And Therapeutic Targeting Of Metabolic Alterations In Estrogen Receptor-Positive Breast Cancer, Steven Tau

Dartmouth College Ph.D Dissertations

Estrogen receptor-positive breast cancer remains one of the most frequently diagnosed cancers in women. Despite adjuvant endocrine therapy reducing the rate of recurrence, there remains a significant proportion of patients whose breast cancer will recur years to decades after the initial diagnosis. The underlying biology of tumor cells in the period between initiation of adjuvant therapy and recurrence is relatively unexplored, preventing the development of better treatment options. From a genome-wide screening approach, we identified and characterized broad metabolic reprogramming associated with the persistent cell state. We demonstrate that a decreased ability to metabolize glucose in turn pushes a higher …

Near-Infrared Paired-Agent Imaging For In Vivo Quantification Of Receptor Occupancy In Tumor, Yichen Feng

Dartmouth College Ph.D Dissertations

Accurate assessment of drug receptor occupancy (RO) holds significant importance in both drug development and personalized medicine, as it facilitates quantitative characterization of dose-response relationship for a given drug. This information not only aids appropriate dose selection for clinical trials but also guides personalized dose optimization in precision medicine.

Molecular imaging, utilizing receptor-specific imaging agents for tissue visualization, has emerged as the major option for in vivo measurement of tissue RO. Nonetheless, the abnormal yet complex structure and hemodynamics of tumors often lead to substantial non-specific uptake and retention of imaging agents, thus introducing significant bias to RO measurements. To …

Continuous, Platform Purification Of Viral Products Using Aqueous Two-Phase Extraction, Natalie M. Nold

Dissertations, Master's Theses and Master's Reports

Cell and gene therapies, a ground-breaking class of drugs that can heal genetic diseases and cure cancer, often have treatment costs over $1 million. Most cell and gene therapies are delivered using virus-based delivery vectors, which are incredibly expensive to manufacture. Liquid chromatography is the favored method of viral vector purification since it is easily scalable and can be functionalized to purify by biospecificity, charge, hydrophobicity, or size. However, chromatography-based purification processes are highly expensive and usually only recover 20-50% of the viral vectors. There is currently no platform purification strategy for viral vectors due to differences in their surface …

Multi-Functional Gelatin-Dithiolane Hydrogels For Tissue Engineering, Saad Asim

Dissertations, Master's Theses and Master's Reports

Biomaterials that integrate multiple functionalities, mimic the extracellular matrix (ECM) microenvironment to support cellular growth, and adhere robustly to damaged tissues are highly needed to advance tissue engineering. Protein-based biomaterials are promising due to their inherent biocompatibility, biomimicry, biodegradation, and cell-supportive properties. Herein, by leveraging the unique ability of dithiolanes to generate on-demand in-situ thiols, we developed a new class of dithiolane-modified, protein-based biomaterial that combines unique, seemingly opposing functions for tissue engineering. Dithiolane-modified gelatin, a model protein used herein, enabled photoinitiator-free photo-crosslinking to form multi-functional gelatin-dithiolane (GelDT) hydrogels, which displayed exceptional long-term stability in cell culture media (>28 …

Contribution Of The Transcription Factor Red Nac To Low Nitrogen Phenotypes In Poplar, Shane Alexander Spence

Dissertations, Master's Theses and Master's Reports

Greenhouse grafting and field trials were conducted to examine the contribution of the RED NAC transcription factor on low nitrogen phenotypes in hybrid poplar (P. tremula x P. alba). Reciprocal grafting trials showed both root and shoot expression contribute to phenotype under low nitrogen conditions, and shoot expression has a leading role in contribution to low-nitrogen phenotype. The impact of RED NAC expression level on auxin polar transport was investigated using a DR5::GUS auxin-responsive reporter gene line as rootstock for RED NAC modified scions. Pilot studies indicate the rate of auxin transport from apex to roots is slower through shoots …

A Multimolecular Perspective On The Success Of Wolf Recovery And Relocation, Samuel D. Hervey

Dissertations, Master's Theses and Master's Reports

With global biodiversity in decline and many species isolated in fragmented habitats, conservation efforts increasingly rely on natural recolonization and translocation to restore ecosystems. These strategies face challenges such as genetic erosion and habitat limitations, highlighting the need for effective monitoring practices. This research assesses the success of wolf recovery initiatives by examining long-term genetic and genomic health using both traditional and advanced molecular methods. First, we investigated the genetic health of 19 gray wolves (Canis lupus) relocated to Isle Royale National Park to restore ecosystem balance. Initial genetic assessments with microsatellites found that these wolves had genetic diversity metrics …

Analytical And Machine Learning Modeling Of Direct Carbonation Of Natural Silicate Minerals, William Hanson

Dissertations, Master's Theses and Master's Reports

The climate-altering effects of atmospheric CO₂ make it necessary to develop innovative technologies that can remove it from the atmosphere. Mineral carbonation is a scalable technology to sequestrate and store CO2 in a stable carbonate mineral form. The efficiency of the mineral carbonation process depends on both feed mineralogy and process parameters. Comprehensive modeling and prediction of mineral carbonation efficiency for different CO₂ – reactive rocks has not been achieved in the past. In this thesis work, analytical and machine learning models are developed to predict the carbonation efficiency of dunite reactions. It is found that random forest …

Autonomic Modulation Of Cardiac Function In Hypertrophic Cardiomyopathy, Jenna Rose Disser

Dissertations, Master's Theses and Master's Reports

Hypertrophic cardiomyopathy (HCM) is the most common genetic heart disorder. HCM is characterized by cardiac hypertrophy, fibrosis, and an increased risk of fatal arrhythmias and sudden cardiac death. Previous studies in humans with HCM have demonstrated increased cardiac norepinephrine spillover and reduced beta-1 receptor (β1R) expression in the left ventricle (LV). We have previously demonstrated that cardiac sympathetic tone is elevated in an alpha-tropomyosin mutant mouse model of HCM. We hypothesized that HCM mice would demonstrate reduced β1R expression in the LV and attenuated heart rate (HR) and dP/dt max responses to ramped infusion of the β1R agonist, dobutamine. …

Implementing Associative Learning Using Neuromorphic Robot, Vinay Kumar Pillalamarri

Dissertations, Master's Theses and Master's Reports

Associative learning, a key cognitive process seen across the animal kingdom, enables organisms to form connections between stimuli and adapt their behaviors based on past experiences. A particularly powerful example is fear conditioning, where animals learn to associate a neutral stimulus with an aversive one, allowing them to predict and avoid potential threats. Inspired by this mechanism, this project implements associative learning on an unmanned ground vehicle (UGV) to develop adaptive behavior through neuromorphic principles. Utilizing Nengo for neural modeling, the UGV learns to associate visual (red color) and tactile (vibration) stimuli through Hebbian learning, a biologically inspired synaptic adaptation …

Social Interactions And Spatial Structure Drive Community Assembly Of Bacterial Biofilm, Matthew C. Bond

Dartmouth College Ph.D Dissertations

Despite the critical nature which microbial communities play in the natural world and human civilization, the breadth of understanding remains shallow. Challenged by scale, high variability between environments, and extensive diversity, microbial ecologists strive to understand connections between a community’s structure and function, as well as the ecological and evolutionary mechanisms underlying observed natural patterning of communities. Biofilms are the predominate mode of growth for microbial communities—characterized by cellular attachment to a surface via a self-produced matrix and heterogeneous structure, often resulting in a primary growth front along the biofilm surface due to differential access to bulk nutrients (cite). This …

Using Fiddler Crab Species To Identify Coastal Biomes Around The Perimeter Of The Atlantic Ocean, Amber Finke

Honors Program Theses

Organisms are distributed differentially across world’s surface based on many environmental characteristics. What part do physical characteristics play in demarcating the distribution of species? Using several international fiddler crab collections and GIS-linked sets of habitat factors, I sought an answer to this question. Geographic distribution data for 23 species from over 1700 collecting sites situated along the coasts of Europe, Africa, North America, Central America, the Caribbean Islands, and South America were used to characterize coastal biogeographic biomes around the perimeter of the North and South Atlantic Ocean. As an initial objective, the exact geographic distribution of each species based …

Toward Circular Economy: Potentials Of Spent Coffee Grounds In Bioproducts And Chemical Production, Hisham Ahmed, Rasaq S. Abolore, Swarna Jaiswal, Amit K. Jaiswal

Articles

With growing concern over environmental sustainability and dwindling fossil resources, it is crucial to prioritise the development of alternative feedstocks to replace fossil resources. Spent coffee grounds (SCGs) are an environmental burden with an estimated six million tons being generated on a wet basis annually, globally. SCGs are rich in cellulose, lignin, protein, lipids, polyphenols and other bioactive compounds which are important raw materials for use in industries including pharmaceuticals and cosmetics. Furthermore, the energy sector has the potential to capitalize on the high calorific value of SCGs for biofuel and biogas production, offering a sustainable alternative to fossil fuels. …

From Instagram To Infowar: The Weaponization Of Social Media And Its Consequences, Grayson Shade Walker

Emory International Law Review

No abstract provided.

Exploring The Potential Of Soy-Based Materials: Sustainable Textile Design And Apparel Production, Kasandra L. Wright, Lance Cheramie

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

This study investigated the potential of soy-based materials as a sustainable alternative in fashion design, focusing on creating textiles using batik techniques. The process involved producing soymilk, bonding pigments, repurposing soy pulp, employing batik methods, and enhancing textures with soy fibers. The resulting sample showcased a visually captivating moth motif on soy textile, demonstrating soy materials' aesthetic and environmental potential. The findings affirm the viability of soy textiles in fashion, offering a biodegradable, renewable option with reduced water and chemical usage compared to traditional materials. Further research is recommended to explore additional design applications and commercial feasibility, contributing to the …

Resistance Proceduralism: A Prologue To Theorizing Procedural Subordination, Portia Pedro

Faculty Scholarship

Several legal scholars have discussed the role of slavery within their own family histories and a growing number of scholars are exploring the successes and strategies of lawyers and Black litigants in freedom suits and other litigation in the United States antebellum South. I build on these literatures with a focus on procedure. In this Article, I analyze procedures involved in a few of my ancestral and personal experiences. Some of the experiences with process involved litigation to be free from slavery while other experiences did not explicitly involve any law. But they all involved process.

Engaging in this practice—marshaling …

A Study Of Inhibition Development In Young Children, Ai Thi Thanh Hoang

College of Education Theses and Dissertations

Child inhibition can be learned consciously and unconsciously, and it is highly dependent on the quality of interaction with adults (i.e., parents and teachers). However, little is known about the impact of teachers’ emotional management and sensitivity on the development of inhibition skills among students in early childhood classrooms. This dissertation takes an integrative approach to explore whether teacher-child relationship and teacher self-efficacy have a bidirectional connection and how they work to impact child inhibition, directly and indirectly. The present research utilizes the large nationally represented Early Childhood Longitudinal Study 2010-2011, sponsored by the National Center for Education Statistics in …

Stable Isotope Analysis Of Manatee Vibrissae To Investigate Individual Patterns Of Resource Use In The Northern Gulf Of Mexico, Levette Tucker, Mackenzie L. Russell, Elizabeth E. Hieb, Ruth H. Carmichael, Carl S. Cloyed

Gulf and Caribbean Research

No abstract provided.

Advancing The Blue Economy Through Sustainable Mariculture: The Prospect Of Pearl Oyster And Sponge Cultivation In St. Vincent And The Grenadines, Kevin Antonio Providence

Gulf and Caribbean Research

The Blue Economy is estimated to be worth over USD 2.5 trillion annually, or almost 3% of the world’s GDP in 2020. St. Vincent and the Grenadines (SVG) in the Caribbean region has a vast and rich marine space that is underutilized. This study aimed to explore the feasibility of pearl oyster and sponge farming to advance the country’s Blue Economy through sustainable mariculture. A qualitative approach was applied, using a literature review and thematic analysis of data gathered through semi—structured interviews and focus group discussions with relevant stakeholders. Among several species evaluated, the result of the study revealed that …

Stable Isotope Analysis Of Antipatharian Diets From The Northwestern Gulf Of Mexico, Keir J. Macartney, Victoria Salinas, Zachary Rivera, David Hicks

Gulf and Caribbean Research

Antipatharians, or black corals, form a key component of the benthic fauna found throughout the Gulf of Mexico (GOM). In the northwestern GOM, they are a common member of the communities found on the natural relic coral—algal banks known as the South Texas Banks as well as on artificial reefs. They are known to provide important habitat for many ecologically and economically important species in the area but are relatively understudied. Here we present a study on their trophic ecology using stable isotope analysis of carbon and nitrogen from 2 species, Antipathes atlantica and Stichopathes luetkeni from a mesophotic artificial …

A Machine Learning-Based Wrapper Method For Feature Selection, Damodar Patel, Amit Saxena, John Wang

Department of Information Management and Business Analytics Faculty Scholarship and Creative Works

This paper presents a two-stage feature selection scheme using machine learning techniques. In the first stage a wrapper method is adopted to select various combinations of subsets of features from the original dataset. The performance of the model is evaluated by three classifiers: K-Nearest Neighbor (KNN), Support Vector Machines (SVM), and Random Forest (RF). In the second and final stage, a sequential backward feature selection Method is applied. The proposed method is demonstrated on eighteen datasets and the average classification accuracy of eighteen datasets achieved is 89.81%, 87.55%, and 89.82% using the KNN, SVM, and RF classifiers, respectively with a …

Rivers Of Language Across Oceans: Review Essay Of River In An Ocean: Essays On Translation, Edited By Nuzhat Abbas, Luise Von Flotow

Journal of Feminist Scholarship

This article presents an academic review of the collection of articles “on translation” and entitled river in an ocean. It engages with the foreword by Françoise Vergès and the introduction by editor Nuzhat Abbas, which set the tone of ‘decolonial feminism’ that permeates the essays. The reviews of the eleven essays—by women from southeast Asia, Africa, Palestine and Saudi Arabia—come from the perspective of feminist translation studies but pay careful attention to their very specific concerns around exile and life in diaspora.

We Shall Overcome: The Evolution Of Quotas In The Land Of The Free And The Home Of Samba, Stella Emery Santana

Seattle University Law Review

When were voices given to the voiceless? When will education be permitted to all? When will we need to protest no more? It’s the twenty-first century, and the fight for equity in higher education remains a challenge to peoples all over the world. While students in the United States must deal with the increase in loans, in Brazil, only around 20% of youth between the ages of twenty-five and thirty-four have a higher education degree.

The primary objective of this Article is to conduct an in-depth comparative analysis of the development, implementation, and legal adjudication of educational quota systems within …

Pacific Islands And The U.S. Military: The Legal Borderlands Of The Environmental Movement, Sonia Lei

Seattle University Law Review

Climate change remains an urgent, ongoing global issue that requires critical examination of institutional polluters. This includes the world’s largest institutional consumer of petroleum: the United States military. The Department of Defense (DoD) is a massive institution with little oversight, a carbon footprint spanning the globe, a budget greater than the next ten largest nations combined, and overly generous exemptions to environmental regulations and carbon reduction targets. This Comment examines how this lack of accountability and oversight plays out in the context of three Pacific islands that have hosted U.S. military bases for decades. By considering the environmental impact of …

Shifting Shapes: Transnational Tactics Of The Authoritarian Right In Brazil And Russia, Erin Katherine Krafft, Caroline De Souza

Journal of Feminist Scholarship

Global right-wing anti-gender movements have developed a transnational pattern in their uses of language as a means of subjugation and of control, and this article examines manifestations of this pattern in two specific states: Brazil and Russia. By exploring the anti-gender languaging, rhetoric, and legislation advanced by Jair Bolsonaro (President of Brazil 2019–2022) and Vladimir Putin (President of Russia 2000–2008 and 2012 to the present) and their supporters, we find that, in both contexts, anti-gender campaigns are reactions to and also drivers for increasingly dramatic political and social conflicts, and in each case, anti-gender ideologies are embedded in larger right-wing …

Cave Music, Ian Sowerwine

Celebrating Writers and Writing in our Communities

No abstract provided.

Celebrating Writing In Our Communities: An Anthology Of The Winning Entries Of The Redwood Writing Contest 2024

Celebrating Writers and Writing in our Communities

No abstract provided.

Trundle On The Ranch, Leola Griffith

Celebrating Writers and Writing in our Communities

No abstract provided.

September 11, 2001, Gracelyn Cox

Celebrating Writers and Writing in our Communities

No abstract provided.

Rocks, Izelle Magana

Celebrating Writers and Writing in our Communities

No abstract provided.