Digital Commons Network^™

Evaluation Of Microglia’S Structural Variations Within An Organotypic Hippocampal Slice Model After Regionalized Toxic Injury, Jesus Antonio Trejos

Theses and Dissertations

The dendritic cells of the CNS, the microglia (MG), are an initiation point of the immunological response within the post blood-brain barrier (BBB) compartment. Microglia morphology drastically changes in response to cell stress to a much different non-dendritic morphology. This investigation postulates that if the first MG responses to toxic injury are isolated and studied in greater morphological detail there is much to be learned about MG metamorphosis from a baseline (M2) to an activated (M1) state. The organotypic hippocampal slice was the experimental setting used to investigate microglial response to toxic injury. This isolates dendritic cellular responses and associated …

Insulin And Dma Suppress The Innate Immune Response Against Aav, Tianxiang Qi

Theses and Dissertations

Adeno-Associated Virus (AAV) is extensively utilized in various clinical trials and approved gene therapies. Despite its widespread use, the immune response to AAV vectors remains a significant limitation. Extensive research has been conducted to mitigate adaptive immune challenges, such as pre-existing antibodies and inhibiting the adaptive immune response. This study, however, centers on the innate immune response elicited by AAV, exploring the modulatory effects of insulin and DMA. Initially, we examined the impact of DMA and insulin on the human liver cell line Hep3B and the human macrophage cell line U937 by culturing together in transwell inserts. Following stimulation with …

Interplay Between Rna Editing And Alternative Splicing Modulates Biophysical And Pharmacological Properties Of The Ion Channel Cav1.3, Willy Munyao

Theses and Dissertations

Alternative splicing and adenosine-to-inosine (A-to-I) RNA editing are post-transcriptional mechanisms that promote proteomic diversity in the brain. The impact of RNA editing on brain physiology is underscored by its association with the etiology and pathogenesis of various neurological and neurodegenerative diseases. Alternative splicing of exon 43 within the CaV1.3 C-terminus produces long (CaV1.3L) and short (CaV1.343S) variants exhibiting distinct gating and pharmacological properties. While the physiological role of CaV1.3 A-to-I RNA editing in brain function is still unfolding, recent findings indicate that unedited CaV1.3 channels display larger calcium influx and enhanced neuronal excitability, suggesting that A-to-I RNA editing may act …

Mobocertinib Antagonizes Multidrug Resistance In Abcb1- And Abcg2-Overexpressing Cancer Cells, Xingduo Dong

Theses and Dissertations

The resistance of cancer cells to multiple drugs, known as multidrug resistance (MDR), remains a significant obstacle that leads to the ineffectiveness of cancer chemotherapy. The overexpression of ATP-binding cassette (ABC) transporters, particularly ABCB1 and ABCG2, has been strongly correlated with MDR. There is an urgent need to explore and identify novel inhibitors targeting ABCB1 and ABCG2 to overcome the related MDR. Mobocertinib (TAK-788) is an orally administered, irreversible EGFR/HER2 inhibitor approved for the treatment of adult patients with locally advanced or metastatic non-small cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR) exon 20 insertion mutations. This study …

The Condor, Dominique Bussell

Celebrating Writers and Writing in our Communities

No abstract provided.

Song Of A Starving Mother, Shayla Frandsen

Inscape

“Please eat,” my husband will beg me. “Can I make you a sandwich?” I wish it were as simple as a sandwich. Nobody wishes more than me. I look at him and say, “I can’t.” I say, “Not right now.”

A Backpack In The River, Shamae Budd

Inscape

That morning, we awoke to the silence of snow—the first snowfall that winter, like white gauze, an inch thick on the cars and lawn and pavement, exhilaratingly blank after the red and yellow color-chaos of autumn. I looked out the window and pulled my winter boots from the closet, tugging my husband to join me for a walk before breakfast. Two months married, we bundled ourselves into knitted hats and scarves and gloves and made our way to the river trail, listening to the muffled sound of our feet leaving prints in the snow.

Creeping Things, Shamae Budd

Inscape

I lay in bed a few weeks ago, admiring the flatness of my stomach, grieving in advance the lack-of-flatness I can expect when my husband and I decide to start growing children of our own, and I found myself wondering, strangely, how snakes are born. Do they hatch from eggs, or are they born live and wriggling?

God Is A Poet, Matthew Wickman

Inscape

Why do we need literature? Certainly, there is much to admire in thoughtful, beautifully crafted narratives, poems, and plays, in gripping stories, layered characters, and poignant turns of phrase. But need is more intense than admiration, more desperate than mere affection. A graduate school friend and Church member once remarked to me, astutely, that if one surveys all the world’s civilizations over the course of known history, one can find plenty of examples of societies that had no concept of private property or insurance industries or professional sports teams or universities or lawyers or finance capitalists or plastic surgeons (and …

Undergraduate Catalog Of Studies, 2023-2024, University Of Arkansas, Fayetteville

Undergraduate Catalog of Studies

No abstract provided.

Logos-Sophia, Elliott Norman, Donald Wayne Viney, Keith Elliott Perkins, Addyson Kay Campbell, Hunter Hinds, Scott Squires

LOGOS-SOPHIA: The Journal of the PSU Philosophical Society

Logos-Sophia, Volume 17, Spring 2024. The Journal of the Pittsburg State University Philosophical Society has largely been a student publication with occasional faculty contribution

Stealing, Scavenging, Or Storing? Breeding Behaviors Of Unisexual Ambystoma Salamanders, Jane Venezia

Master's Theses and Doctoral Dissertations

The unisexual Ambystoma salamander complex is a lineage of all-female salamanders that use a unique form of reproduction: kleptogenesis. Kleptogenesis requires sperm from male salamanders of co-occurring sexual species, potentially putting unisexual and sexual females in breeding competition. We investigated if unisexual salamanders require courtship from sexual males or if they can use sperm left over from previous breeding events. We put unisexual salamanders into boxes containing spermatophores from a previous courtship and examined if they laid viable eggs. We also genotyped adults and embryos to assess parentage. Some of the unisexuals in the boxes produced viable eggs; however, the …

Increasing The Efficacy Of Actinomycin D With Resveratrol In Aerodigestive Tract Cancers, Lukmon Morenikeji Raji

Theses, Dissertations and Capstones

Chemotherapy poses a significant challenge for cancer patients due to drug-associated toxicity, which often results from their effects on both healthy (normal) and cancerous cells. While various options aim to reduce toxicity and optimize beneficial effects, a comprehensive solution remains elusive. Cyclotherapy is one such approach developed to protect normal cells from the toxic effects of chemotherapy drugs. The basic principle underlying cyclotherapy is p53- dependent cell cycle arrest of normal cells while killing cancer cells via a p53-independent mechanism using a second drug. In our research, we investigated the inhibitory effects of a combination of two low-dose anticancer drugs, …

Have Some Heart For The Heartland: A Call For A Federal Right To Repair Law, Gabriel Dominic Gomez

Journal of Legislation

No abstract provided.

On Traditionalism In Free Speech Law, R. George Wright

Journal of Legislation

No abstract provided.

Marine Protected And Conserved Areas: Beneficial Uses Of Artificial Intelligence, Kyla Lucey

Catholic University Journal of Law and Technology

The ocean is an invaluable tool to the survival of humankind and “produces half of the world’s oxygen, absorbs and sequesters one third of the carbon dioxide human activities emit, provides protection from extreme weather events, and provides a source of food and livelihoods.” Without it, communities would suffer, animals would die off, industries would disappear, and the world would be much worse off. The recommendations made here reflect the growing concern the world has adopted regarding the climate crisis. This concern is warranted as many animals have already disappeared, plants are dwindling, and the once wild areas of the …

Mark-Recapture Methods In Eastern Diamondback Rattlesnake (Crotalus Adamanteus) Monitoring, Carissa J. Adams

Theses, Dissertations and Capstones

Eastern diamondback rattlesnakes (Crotalus adamanteus; EDB) are an imperiled species native to the Southeastern United States longleaf pine (Pinus palustris) ecosystem. Several markrecapture methods have been used range-wide in hopes of increasing knowledge on their population numbers. I explored the efficacy of two mark-recapture methods (scale cauterization and dorsal head photography) using the Marine Corps Recruit Depot, Parris Island, SC EDB population in this multi-part study. The first method I evaluated was ventral scale branding, which has been widely implemented without knowledge of brand permanence. I classified misidentification errors at capture status (new versus recapture) and …

Management Considerations In A Case Of Sepsis Induced Takotsubo Cardiomyopathy In A Post-Operative Patient., Avraham Fox, Rachel Groening, Carsten Z. Steinmetz, Callaham A. Brock, Saptarshi Biswas

South Atlantic Division GME Research Day 2024

No abstract provided.

Relationship Building Advice For Liaison Librarians: Putting It Into Practice And Troubleshooting, Bettina Peacemaker, Kelsey Cheshire, Janet M. Reid, Carla-Mae Crookendale, Julie Arendt, Sergio Chaparro, John Glover, Stephani Rodgers, Erin Carrillo

VCU Libraries Faculty and Staff Publications

Liaison librarians depend on communication and cooperation to be successful, so professional publications about liaison librarianship regularly include discussions of relationship building methods. As a department of experienced liaison librarians, we identified the relationship-building ideas from previous publications that were proactive in nature and had worked for us. Even though the advice has been helpful, we faced challenges or heard about challenges from other librarians when we discussed implementing the ideas. To improve on the published ideas, we have added tips for putting them into practice and answered troubleshooting questions based on our experiences. Adapting to and working around challenges …

Effects Of (2r,6r)-Hydroxynorketamine In Assays Of Acute Pain-Stimulated And Pain-Depressed Behaviors In Mice, Todd M. Hillhouse, Kaitlyn J. Partridge, Patrick I. Garrett, Sarah C. Honeycutt, Joseph H. Porter

Psychology Publications

Ketamine has been shown to produce analgesia in various acute and chronic pain states; however, abuse liability concerns have limited its utility. The ketamine metabolite (2R,6R)-hydroxynorketamine (HNK) has been shown to produce antidepressant-like effects similar to ketamine without abuse liability concerns. (2R,6R)-HNK produces sustained analgesia in models of chronic pain, but has yet to be evaluated in models of acute pain. The present study evaluated the efficacy of acute (2R,6R)-HNK administration (one injection) in assays of pain-stimulated (52- and 56-degree hot plate test and acetic acid writhing) and …

Annual Report, 2023-2024, University Of Arkansas, Fayetteville. School Of Continuing Education And Academic Outreach

School of Continuing Education and Academic Outreach - Global Campus Annual Report

No abstract provided.

In The Eyes Of The Beholders: Understanding Public Perceptions Of Dungeons And Dragons And Its Players Using Visual Media, Mallory Hardesty

Mahurin Honors College Capstone Experience/Thesis Projects

Television can have a major impact on how groups of people are seen in shows. The media that people consume have an influence on how they view and interact with the world. Television also utilizes character creation of different types of people to create an array of characters for viewers to enjoy. However, how these characters are portrayed can influence how the public sees the groups that the character is apart of. The “Nerd” subgroup started for form as a subcultural identity in the 1970s and 80s, and as this group began to form, stereotypes about them quickly began to …

Estimating Plant Β-Diversity Using Airborne And Spaceborne Imaging Spectroscopy, N. P. Kamaraj, H. Gholizadeh, R. G. Hamilton, S. D. Fuhlendorf, K. F. Huemmrich, J. A. Gamon

School of Natural Resources: Faculty Publications

No abstract provided.

The Redwood, V.120 2023-2024, Santa Clara University

The Redwood

No abstract provided.

Ms Environmental Biology Capstone Project, Reilly Miller

Regis University Student Publications (comprehensive collection)

Habitat loss due to deforestation is a primary threat to global biodiversity. Clearing tropical rainforests for agriculture leads to forest fragmentation. Forest fragments contain fewer large trees and provide lower food availability for primates compared to continuous forests. Mantled howler monkeys inhabit the increasingly fragmented rainforests of Central and South America and may need to alter their activity and spatial cohesion to mitigate competition and preserve energy in fragments where there is lower quality food. We compared howler monkey activity and spatial cohesion across a small forest fragment (La Suerte Biological Research Station, LSBRS) and a large, continuous forest (La …

Technē Logos, Care And The (Neg) Anthropocene The Second Annual Conference Of The European Culture And Technology Laboratory., Noel Fitzpatrick, Connell Vaughan

Conference Proceedings

The second ECT Lab+ annual conference took place in the East Quad building of the Grangegorman campus of TU Dublin in January 2023 and this publication is an edited collection of papers, keynotes, and artistic interventions that were delivered over the three days of the conference. As the first iteration of the conference was forced to be held online due to the public health restrictions of the Covid-19 pandemic, this was the first annual conference to take place in person. A total of 40 speakers presented work over the course of the conference. Despite the return to in-vivo modalities, the …

International Standards Regarding Child Marriage And African Legal Systems, John Mukum Mbaku

California Western International Law Journal

No abstract provided.

Using Edna And Traditional Methods To Assess Fish Diversity In Narragansett Bay, Juliane Mora

Open Access Master's Theses

Coastal ecosystems are degrading rapidly, and molecular analysis of environmental DNA (eDNA) has become an increasingly popular method to quantify changes in biodiversity, and as a powerful and affordable complement to monitor aquatic environments. Yet, side by side comparisons of biodiversity estimates from eDNA and other traditional methods are needed to understand the level of accuracy of the eDNA method. We used eDNA metabarcoding of the 12S mitochondrial gene to generate a multi-species fish biodiversity assessment of Narragansett Bay (NB). Here we estimated biodiversity using eDNA data and compared it to conventional seine surveying data. We then further estimate biodiversity …

Epigenetics And Character Virtue Development, David S. Moore

Pitzer Faculty Publications and Research

Current understandings of epigenetics offer some insights into the developmental origins of character virtues. Research on epigenetics has demonstrated that phenotypes depend on both genomic and non-genomic information for their development, and such is true for the development of character virtues as well. This conclusion offers significant support for the Developmental Systems perspective. Although researchers have not yet studied the role of molecular epigenetic factors in the development of character, the field of behavioral epigenetics has revealed how such factors contribute to psychological and other behavioral phenotypes in general. In particular, behavioral epigenetics research can highlight the plasticity of human …

Older Adult Compendium Of Physical Activities: Energy Costs Of Human Activities In Adults Aged 60 And Older, Erik A. Willis, Stephen D. Herrmann, Mary Hastert, Chelsea L. Kracht, Tiago V. Barreira, John M. Schuna Jr., Zhenghua Cai, Minghui Quan, Scott A. Conger, Wendy J. Brown, Barbara E. Ainsworth

Kinesiology Faculty Publications and Presentations

Purpose: To describe the development of a Compendium for estimating the energy costs of activities in adults ≥60 years (OA Compendium).

Methods: Physical activities (PAs) and their metabolic equivalent of task (MET) values were obtained from a systematic search of studies published in 4 sport and exercise databases (PubMed, Embase, SPORTDiscus (EBSCOhost), and Scopus) and a review of articles included in the 2011 Adult Compendium that measured PA in older adults. MET values were computed as the oxygen cost (VO₂, mL/kg/min) during PA divided by 2.7 mL/kg/min (MET₆₀₊) to account for the lower resting metabolic rate …