Digital Commons Network^™

Mitogenomic And Physiological Comparison Between Wild And Domesticated Congeners, Nicholas Enriquez

Open Access Theses & Dissertations

Domestication is a process in which wild populations are morphologically and/or behaviorally manipulated through selective breeding to become optimized for living in captivity. Consequently, many traits become quickly and significantly diverged from their ancestral, wild lineages, a process known as part of domestication syndrome. Captive breeding of mallards (Anas platyrhynchos) was one of the last major successful domestication events resulting in dozens of recognized breeds today. Many breeds were optimized for either ornamental or agricultural purposes (i.e., meat and/or egg production), but the game-farm mallard was specifically bred for sport hunting. In North America, the use of game-farm mallards to …

Application Of Path Analysis To Determine The Impact Of Age And Sex On The Neural Circuits That Promote Nicotine Withdrawal Severity, Veronika Espinoza

Open Access Theses & Dissertations

The rising prevalence of electronic cigarette (e-cigarette) use presents a major public health concern, particularly in adolescent females who are most susceptible to nicotine use and its long-term negative health consequences. The goal of this dissertation was to utilize preclinical rodent models to understand how group differences in age and sex influence neural activation patterns in brain circuits believed to promote nicotine withdrawal. Prior pre-clinical work, conducted largely in male rodents, has established that there are two major brain pathways that modulate the behavioral effects of nicotine withdrawal. These include the mesolimbic and medial habenula-interpeduncular nucleus (MHb-IPN) pathways. To assess …

Development Of A High-Throughput Framework For Studying Choice-Movement Dynamics In Freely Moving Rats Across Motivational And Pharmacological States, Atanu Giri

Open Access Theses & Dissertations

This thesis develops and applies high-throughput behavioral assays and analysis pipelines to study how external contingencies and internal state shape rodent decision-making. First, it introduces RECORD (Reward-Cost in Rodent Decision-making), a modular platform that combines 3D-printed arenas, microcontroller-based control, and closed-loop trial structure to deliver graded rewards and costs in a foraging-like environment. RECORD supports scalable data collection across multiple arenas and a software stack for parsing, databasing, and extracting spatiotemporal behavioral features and psychometric choice functions. Using this framework, the thesis quantifies how animals integrate sucrose reward magnitude with aversive light cost, revealing structured individual differences and sex-dependent movement …

The Mouse At The Border: Magic, Money, And Manufactured Identity, Ricardo Manuel Hinojosa

Open Access Theses & Dissertations

This thesis investigates the methods by which The Walt Disney Company constructs and negotiates representations of Latine, gender, and queer identities within contemporary animation, with a particular emphasis on Coco (2017), Encanto (2021), and Wish (2023). Through a critical and intersectional lens, this research posits that Disney strikes a balance between cultural specificity and global market appeal, often leading to representations that are visually elaborate yet narratively limited. Both Coco and Encanto showcase a significant engagement with cultural traditions and themes such as memory and generational trauma; however, they ultimately fall short of thoroughly addressing systemic issues like colonialism and …

Metabolomics In The Dryland Critical Zone: The Role Of Soil Metabolites In Phosphorus Acquisition, Kalpana Kukreja

Open Access Theses & Dissertations

Earth's Critical Zone (CZ) plays a key role in sustaining life through carbon, water, and nutrient cycling from the top of the canopy to the groundwater. A key feature of critical zone research is the integration of multiple interdisciplinary techniques. An emerging set of techniques that can add to this approach is the field of metabolomics, the quantification and study of comprehensive metabolite profiles. In this dissertation, I apply metabolomics to address knowledge gaps in dryland critical zone ecosystems, specifically how soil metabolite dynamics influence biogeochemical processes, and with a particular emphasis on phosphorus (P) cycling in the Chihuahuan Desert. …

Microcosms & Warscapes, Heather J. Lathers

Open Access Theses & Dissertations

This poetry collection features sensory-centered writing and common objects, scenes, and interactions to authentically capture the multi-perspective and nuanced experiences of wartime. Detailed language is critical when poets become mouthpieces for those enduring war's pain. Focusing closely on items such as teacups, boots, bones, mud, rice, and rats creates a kind of intimacy not often found in war writing. Specific conflicts addressed include World War I, Vietnam, and The Cambodian Genocide. The poems here, in various forms and voices, aim to spotlight the lost, marginalized, abused, and forgotten-- the much-needed humanity war stories tend to neglect. Ultimately, these pieces seek …

Public Art And The Future Of Remembrance In Contested Spaces, Rebecca Ann Munoz

Open Access Theses & Dissertations

This dissertation examines the memorial landscape of El Paso, Texas as a contested site of public memory, cultural identity, and community representation. Situated at the U.S.-Mexico border, El Paso functions as a palimpsest of layered histories shaped by colonization, militarization, and ongoing negotiations of belonging. Drawing on memory rhetoric and composition studies, and art history, the paper raises critical questions about community engagement, representational equity, and the politics of visibility in public art processes. The paper ultimately calls for a deeper examination of the rhetorical strategies and institutional frameworks that shape memorial meaning-making at the local level, with broader implications …

Mapping The Lateral Hypothalamic Area: Chemoarchitecture, Connectivity, And Activation In Motivated Learning And Reward, Vanessa Inez Navarro

Open Access Theses & Dissertations

The lateral hypothalamic area (LHA) is a large and complex hypothalamic region with diverse functions across four main rostrocaudal divisions. Despite its size and diversity, disagreements over how to define LHA subdivisions (e.g., Swanson atlas lists 17 subdivisions) make it challenging to fully characterize its structure and function. Across these subregions, neuronal connectivity varies, indicating they serve different behavioral functions. Consistent with this, particular LHA neuronal pathways have been linked to reward-related responses, emphasizing the region's roles in motivation, feeding, and cognition. Current approaches to classifying the LHA have primarily relied on neurochemical markers or gene-directed manipulations, often without integrating …

Novel Roles Of G-Beta-Gamma Signaling In Microtubule Dynamics And Neurodegeneration, Diana I. Olivas

Open Access Theses & Dissertations

Neurodegenerative disorders (NDDs), including Alzheimer's disease (AD), are characterized by progressive neuronal dysfunction, cytoskeletal disruption, and cognitive decline. Although Tau hyperphosphorylation and neurofibrillary tangle (NFT) formation are classical hallmarks of AD, emerging evidence suggests that microtubule (MT) dysfunction may precede and occur independently of Tau pathology. Understanding upstream regulators of MT dynamics is therefore critical for elucidating early mechanisms of neurodegeneration. Previous work from our laboratory demonstrated that G protein βγ (Gβγ) subunits directly interact with tubulin and promote MT assembly. However, the mechanism of Gβγ signaling in neuronal cytoskeletal stability and neurodegeneration remained poorly understood. The present study investigated …

Drug Repurposing Against Giardia Targeting Lipid Rafts, Extracellular Vesicles, And Sphingolipid Synthesis, Breanna Chantal Pence

Open Access Theses & Dissertations

Giardia lamblia is a protozoan parasite responsible for the intestinal disease giardiasis worldwide. Giardia is one of the major intestinal parasites in the world, with over 500,000 new cases annually. Symptoms of giardiasis include diarrhea, abdominal pain, and nausea. Children are particularly vulnerable to giardiasis, with cases of malnutrition, stunted growth, and cognitive impairment in countries where the disease is endemic. Giardia consists of two morphologic forms: an infective cyst which transmits the disease and a motile trophozoite which attaches to the epithelium of the small intestine during infection. Metronidazole is a common treatment for giardiasis, but cases of toxicity …

Computational Studies Of Alpha-Lactalbumin Binding To Perfluorodecanoic Acid, Randhal Smith Ramirez Orozco

Open Access Theses & Dissertations

This thesis investigates the structural and thermodynamic interactions between Perfluorodecanoic acid (PFDA) and bovine alpha-lactalbumin (ALAC), a critical milk protein essential for infant nutrition. The primary objectives were to computationally determine the most probable binding sites of PFDA to ALAC and to quantify the strength of these interactions alongside the structural changes they induce. This study addresses a critical gap in understanding how persistent environmental contaminants like PFDA are recruited and transported by nutritional proteins within the milk matrix. The methodology integrated high-throughput molecular docking with long-range, molecular dynamics (MD) simulations to provide a dynamic characterization of the ALAC-PFDA complex. …

Daughters Of The Road, Kristen Marie Sche

Open Access Theses & Dissertations

The beloved Road Novel has long been a tale submerged in the coming-of-age story following men as they search for themselves along the American highways. The genre can vary from journeys of families seeking refuge or tackling a task to men seeking answers to something missing in their lives. However eclectic the genre, it lacks in the pilgrimage of girls finding what the road can bring to them in a literary fictional context. This thesis provides a novel written in the popular genre while exploring themes of sisterhood, grief, and inclusion through the context of a hybrid form of narration …

Salience Network Circuit Dynamics In Distress Tolerance And Drug-Seeking Vulnerability, Marina Alicia Smoak

Open Access Theses & Dissertations

The psychological distress experienced during drug withdrawal can lead individuals with substance use disorder to abandon their goal of abstinence. Numerous human studies demonstrate that an inability to persist in challenging, goal-directed behavior while experiencing psychological distress is associated with multiple stages of substance use, including relapse. This behavioral construct is referred to as low distress tolerance (DT). Rodent models of DT similarly show that low DT predicts elevated cocaine relapse, particularly when DT is assessed following extended abstinence. This abstinence period corresponds with the incubation of craving, a phenomenon characterized by progressively increased cocaine-seeking over time. Importantly, relapse vulnerability …

High-Resolution Mapping Of Brainstem Feeding Circuits: A Framework For Targeted Neuroscience Tools, Geronimo Tapia

Open Access Theses & Dissertations

The neural control of energy balance is mediated in part by distributed brainstem circuits that integrate metabolic, visceral, and hormonal signals to coordinate appropriate motivated behaviors and autonomic responses. Despite extensive study, the precise neuroanatomical architecture regulating metabolism remains incompletely defined within standardized atlas frameworks. This work addresses that gap through complementary studies examining an acute glycemic challenge methodology known to stimulate feeding, the acute delivery of an anorexigenic-associated neurohormone, and the chemoarchitecture of a major feeding-related region, the parabrachial nucleus. First, utilizing 2-deoxy-D-glucose (2-DG) induced glucoprivation, we map the rapid response of dorsal medullary neuronal populations with high spatial …

Fluoxetine Exposure During Adolescence Alters Prefrontal Cortex Signaling And Nociceptive-Related Behavior In Adulthood, Anapaula Themann

Open Access Theses & Dissertations

The long-term neurobiological consequences of adolescent antidepressant exposure remain poorly understood, despite the substantial increase in selective serotonin reuptake inhibitor (SSRI) prescription rates in youth. Therefore, the goal of this dissertation was to determine whether adolescent exposure to the SSRI fluoxetine (FLX) produces lasting molecular and behavioral adaptations, using rodents as a model system. To do this, in Chapter 2, I assessed whether adolescent FLX exposure (postnatal day [PD] 35-49) alters signaling molecules associated with survival and synaptic plasticity (extracellular signa- regulated kinase [ERK]) within the adult prefrontal cortex (PFC) - a brain region that plays a central role in …

Determining Zap-70 Signaling Pathways Mediated By Ccr7 In Tcr T Cell Activation, Vivian Torres

Open Access Theses & Dissertations

Activation of T lymphocytes is the main event that occurs in the adaptive immune response after engagement of the T cell receptor (TCR) with the peptide of the major histocompatibility complex (pMHC) molecules that are demonstrated by antigen presenting cells [1]. A key step in this process is the recruitment and activation of the zeta chain associated protein of 70 kDa (ZAP-70). ZAP-70 couples TCR engagement to downstream intracellular signaling pathways through phosphorylation of tyrosine residues within intracellular tyrosine activation motifs (ITAMs) [2]. Although there have been extensive studies on the signaling response through TCR activation, the regulatory influence of …

From Soils To Plants And Back Again: The Effects Of Small Mammals On Arctic Ecosystem Processes, Andrew Uccello

Open Access Theses & Dissertations

Herbivores are essential drivers of above and belowground processes in ecosystems across the globe. Through their effects on ecosystem processes, herbivores represent a critical force shaping ecosystems undergoing rapid environmental changes, such as the Arctic. However, there are critical gaps in our knowledge of herbivores as drivers of Arctic ecosystem processes, especially concerning the role of small mammal herbivores. While previous research has made significant strides in identifying the role of small mammal herbivores as both top down and bottom up forces in Arctic ecosystems, we still lack an understanding that explicitly connects plant and soil responses to herbivory, especially …

Characterization Of The Antiviral Activity Of Mouse Schlafen Proteins, Wendy Paola Alvarez Leal

Open Access Theses & Dissertations

The goal of this work was to characterize the anti-viral role of mouse Schlafen8 and Schlafen9. Expression of these proteins is induced by type I interferon in response to viral infections. Importantly, their human functional orthologs exhibit anti-viral functions, and the structural element required for these activities are conserved in Schlafen8 and Schlafen9. Therefore, it is expected that these proteins will also impair viral replication. An important limitation in this field is that the anti-viral function of human SLFNs has been evidenced only in in vitro cellular models. No informative mutations have been described in human populations so far. Thus, …

Simultaneous Carrier, Cargo, And Functional Tracking Of Lipid Nanoparticles, Hannia Vanessa Balcorta Muñoz

Open Access Theses & Dissertations

Lipid nanoparticles (LNPs) have become effective delivery vehicles in nanomedicine, particularly for nucleic acid-based treatments. However, a major limitation remains in the field regarding the simultaneous monitoring of the nanoparticle carrier, its encapsulated payload, and the resulting functional protein production in both in vitro and in vivo systems. Furthermore, traditional lipophilic dyes employed for labeling LNPs frequently exhibit incompatibility with tissue-clearing methods, such as CLARITY, due to signal loss following lipid removal. This research developed and evaluated a new fluorescent labeling method using CM-DiI, a dye that reacts with thiols, for multimodal tracking of LNPs. CM-DiI was included in the …

Language Control Over Structural Representation In Bilinguals, Anahy Barragan-Diaz

Open Access Theses & Dissertations

In daily life, bilinguals sometimes speak each of their languages in situations where the other language cannot be understood. To avoid saying words in the wrong language in such situations, bilinguals inhibit lexical representations from the non-target language (Green, 1998). But do they inhibit structural representations? This study investigated how inhibitory control operates over structural representations in English-dominant Spanish-English bilinguals via a picture description task. An experimental group was asked to describe dative-eliciting pictures in English (Baseline Phase), followed by dative pictures in Spanish (Manipulation Phase), and once again dative pictures in English (Target Phase), while a control group followed …

Soil Property Responses To Push-Pull Cropping In East Africa, Grace Mercy Amboka, Mattias Jonsson, Celina Apel, David Meinhof, Adomas Liepa, Frank Chidawanyika, Andargachew Detebo, Felipe Librán-Embid, Emily A. Martin, Ingolf Steffan-Dewenter, Marcell K. Peters, Jie Zhang, Michael Thiel, Michael Otim, James Mugisha, Ghebremedhin Belay Bahta, Fredah Maina, Alice Murage, Benjamin Feit, A. Sigrun Dahlin

All Peer-Reviewed Publications

Push-pull technology is increasingly promoted in sub-Saharan Africa, particularly for pest management and enhancing crop productivity. However, its influence on soil properties remains understudied, despite its potential implications for soil health and sustainable soil fertility management. This study examines soil properties in push-pull and conventional non-push-pull cropping systems. Soil samples were collected from push-pull and conventional plots in Ethiopia, Kenya, Rwanda, and Uganda. We examined the associations between soil physicochemical properties and cropping systems, along with key components of push-pull, namely Desmodium coverage and plot age, and manure and mineral fertiliser application. Overall, there were a few differences in soil …

Examining The Interactive Effects Of Time Perspective And Life History On Decision-Making Patterns Among College Students: A Modified Replication Of Griskevicius Et Al. (2011b), Cameron M. Robins

Master's Theses

Griskevicius and colleagues (2011b) demonstrated decision-making patterns consistent with life history theory, called life history strategies, using an experimental design in which they randomly assigned college students to read one of two texts. The experimental condition was designed to trigger a feeling of heightened awareness of one’s own mortality, called mortality salience. Participants in this condition exhibited patterns of risk-taking and delay discounting on the outcome measures that were related to their childhood socioeconomic status (SES), one of the first demonstrations of life history strategies in an experimental setting.

However, multiple attempts at replication have failed to show similar results. …

Clinical And Demographic Characteristics Of Hospitalized Patients With Xylazine-Related Wounds, Elizabeth Novick, Nikhil Seval, Dagan Coppock, Mollie Tucker, Daniel Taupin, John Zurlo, Carolyn Kramer

Department of Medicine Faculty Papers

OBJECTIVES: Little is known about the demographic and clinical features of patients with xylazine-related wounds. We sought to characterize hospitalized patients with substance use disorder (SUD) and xylazine-related wounds seen for infectious diseases consultation.

METHODS: We retrospectively reviewed the medical records of 193 patients diagnosed with SUD at 3 Philadelphia hospitals between October 2023 and January 2024. Patients were identified as either being diagnosed with (n=73) or without (n=120) xylazine-related wounds. We compared the demographic and clinical features between the 2 groups.

RESULTS: Patients diagnosed with wounds were younger ( P < 0.001), more likely to be White or Hispanic and less likely to be Black/AA ( P < 0.001), more likely to have tested positive for fentanyl ( P < 0.001), amphetamines ( P < 0.001), and cocaine ( P 0.001), more likely to acknowledge injection ( P < 0.001), and more likely to leave the hospital by patient-directed discharge (PDD) ( P < 0.001). Most patients had multiple wounds (75%), and more than half (53%) had at least one wound greater than 10 cm at its widest dimension. Although most patients diagnosed with wounds received antibiotics, many did not have symptoms or signs of infection.

CONCLUSIONS: Xylazine-related wounds are highly associated with illicitly manufactured …

Soil Microbial Responses To Artifical Light At Night, Randall L. Walker

Open Access Theses & Dissertations

Global change, driven by both natural processes and human activities, has significantly transformed ecosystems. Urbanization and artificial light at night (ALAN) are key contributors, disrupting natural light cycles and influencing biological rhythms, biodiversity, and ecosystem functions. Thus far, there remains a gap in knowledge of how soil ecosystems are impacted by ALAN. This study uses a laboratory incubation to investigate the effects of ALAN on soil microbial communities and functions across urban and natural ecosystems. Briefly, soil was collected from eight sites around El Paso, Texas and neighboring New Mexico representing urban and rural light exposure. Soil was then subjected …

Molecular Mechanisms Of Pfas Induced Protein Dysfunction: Implications For Neurodegenerative Risk, Daisy Lee Wilson

Open Access Theses & Dissertations

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants with widespread human exposure and well-established links to adverse health outcomes. However, despite increasing epidemiological and experimental evidence, the molecular mechanisms underlying PFAS-induced neurotoxicity remain incompletely defined. This dissertation investigates PFAS toxicity across biological scales, integrating protein biophysics with neuronal cell models to elucidate pathways relevant to neurodegeneration. At the molecular level, we demonstrate that PFAS disrupts the structure and function of globular proteins, including the impairment of α-lactalbumin's calcium-binding capacity and of β-lactoglobulin's retinol binding, through concentration-dependent conformational destabilization. By applying these findings to a neuronal model, we show that …

Overcoming Glioblastoma Resistance To Temozolomide With New Derivatives: Design, Synthesis, And Mechanistic Studies, Hualin Zhang

Open Access Theses & Dissertations

Glioblastoma multiforme (GBM) is an aggressive brain tumor with rapid progression and poor prognosis. Although temozolomide (TMZ) remains a central component of standard therapy, its clinical benefit is limited and resistance frequently emerges through DNA repair pathways, particularly O6-methylguanine-DNA methyltransferase (MGMT). Because DNA cross-links are generally more difficult to repair than monoalkylated lesions, we hypothesized that TMZ derivatives bearing protein-reactive functionality might generate damage that is less susceptible to MGMT-mediated resistance. Guided by this rationale, we designed a new class of N3-modified TMZ derivatives containing covalent warheads, developed a synthetic route compatible with the chemical instability of the TMZ scaffold, …

The Secret Science Of Songs. Created By All Children Everywhere, Paul G. Morehouse Phd

The Transdisciplinary STEAM+ Journal

This field article introduces The Secret Science of Songs, a research-informed, practice-based framework that reconceptualizes songs as powerful, transdisciplinary tools in early childhood education. Drawing on over 40 years of classroom-based action research alongside established findings in cognitive science and music perception, the framework argues that young children’s engagement with songs is rooted not primarily in performance skill, but in their innate capacity to perceive and process auditory patterns in music and language. These pattern-based experiences constitute a rich aesthetic and cognitive domain through which children construct meaning, long before mastery of melody or verbal precision emerges.

The article …

Fractsynth: Exploring Glitch Timbres With Chaos Theory, Aidan Roach

The Transdisciplinary STEAM+ Journal

In this paper, I explore how chaos theory can be used to design a new kind of synthesizer with the primary focus of producing glitchy, unpredictable sounds. Glitch music embraces abstract sound design, malfunctioning electronics, and randomness as the main compositional elements. However, most synthesizers rely on stable, repetitive oscillators that often sound too controlled. To challenge this, I developed FractSynth, a real-time synthesizer that uses chaotic attractors–including the Logistic Map, Henon Map, and Lorenz System–as modulation sources for frequency, amplitude, and tone. The software also features real-time Lyapunov Exponent Tracking, which gives users a direct visual of how …

Academic Summaries - Philanthropy, Erica Jensen, Sebastian Dorantes, Faith Manchester, Isabelle Stoddard

Journal of Nonprofit Innovation

No abstract provided.

Va Dent J July, August And September 2020

Virginia Dental Journal

No abstract provided.