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Articles 58141 - 58170 of 741770
Full-Text Articles in Entire DC Network
Unraveling The Elasmobranch: A Look At Shark Oral Microbiomes In South Florida, Varsha Chigurupati, Paul Paronich, Adhvika Saravanan
Unraveling The Elasmobranch: A Look At Shark Oral Microbiomes In South Florida, Varsha Chigurupati, Paul Paronich, Adhvika Saravanan
Undergraduate Student Symposium
The intricate relationship between host organisms and their associated microbial communities, known as the microbiome, has garnered increasing attention across various fields of biology. Sharks, as apex predators in marine ecosystems, are important organisms to study and understand. Previous studies have shown shark gills and shark mouths are abundant in various types of commensal and pathogenic bacteria. This study examines results from a variety of swabs collected from shark species off the east coast of Florida. Through traditional microbial culturing and Next Generation 16S rRNA (Ribosomal RNA sequencing), we will understand the composition and functional significance of the shark microbiome …
What Is The Level Of Knowledge And Awareness Among Healthcare Providers In Neonatal Intensive Care Units (Nicus) Regarding Prelinguistic And Linguistic Development In Premature And Newborn Infants?, Brooke Gallo, Preye Ford
What Is The Level Of Knowledge And Awareness Among Healthcare Providers In Neonatal Intensive Care Units (Nicus) Regarding Prelinguistic And Linguistic Development In Premature And Newborn Infants?, Brooke Gallo, Preye Ford
Undergraduate Student Symposium
Healthcare professionals in the Neonatal/Newborn Intensive Care Unit (NICU) are charged with education and competencies to maximize life support and viability in premature infants and medically fragile term infants. Often, the neonate’s first experiences with communication occur while in the hospital environment. It is well established that neonates and infants who spend more than five days in the NICU are at an increased risk for developmental delays, including language delays, speech sound disorders, motor speech disorders, and/or cognitive delays. Research indicates that 'Up to 50% of infants born less than 32 weeks of gestation develop disadvantaged outcomes, including language and …
Quantifying Respiratory Plasticity Of Checkered Pufferfish Under Climate Change Conditions, Logan Exton
Quantifying Respiratory Plasticity Of Checkered Pufferfish Under Climate Change Conditions, Logan Exton
Undergraduate Student Symposium
Increasing water temperatures resulting from global climate change introduce new energetic demands for marine organisms. Higher energy input will be required to cope with a subsequently higher metabolic rate, affecting all aspects of an individual’s life and therefore their survival. Because estuaries act as a link between rivers and oceans, they and their inhabitants are considered to be the most threatened by climate change. Therefore, it is crucial to understand how these organisms will respond to increased stressors due to climate change. Checkered pufferfish (Sphoeroides testudineus) are among the most common teleost fish in the Indian River Lagoon, …
Stereoscopic 3d Video Games Can Boost Depth Perception, Lara Cereminati
Stereoscopic 3d Video Games Can Boost Depth Perception, Lara Cereminati
Undergraduate Student Symposium
Previous studies have shown that video game play improves a range of visual functions such as contrast sensitivity and visual crowding. Our experiment goal was aimed to test whether playing video games in a virtual 3-dimensional (3D) environment can modify depth perception or not. Altogether, 24 healthy young adults with no previous video game experience were recruited in two groups. In the treatment group (3DVG, n=12), participants were required to play stereoscopic 3D video games for a total of 40 hours over 4-5 weeks. In the control group (2DVG, n=12), participants played the same video games but in 2D mode …
Synthesis And Comparative Analysis Of FeÏNiγSn Magnetic Nanoparticles , Sofia Celis
Synthesis And Comparative Analysis Of FeÏNiγSn Magnetic Nanoparticles , Sofia Celis
Undergraduate Student Symposium
Nano synthesis of Heusler alloys holds promise for their potential applications in creating next generation computer hard drives. This research project aims to investigate the synthesis and comparative analysis of Heusler nanomagnets using Ni2FeSn, Fe2NiSn, and FeNiSn compositions. The nanoparticles are synthesized using microwave-assisted heating and are then subjected to X-ray diffraction (XRD) analysis and SQUID magnetometry. We are particularly interested in conducting comparative analyses of the three nanomagnet compositions in order to determine which demonstrates superior magnetic properties for potential applications in the field.
Synthetic Psychedelic Phenethylamine Drugs After Microtubule Formation And Neurogenesis In Human Neural Progenitor Cells, Amelia Zannoni, Chase A. Gonzalez, Peter Stringer, Roberto G. Fileni, Nitisha S. Gajavalli, Julia Langman, Grace K. Wong
Synthetic Psychedelic Phenethylamine Drugs After Microtubule Formation And Neurogenesis In Human Neural Progenitor Cells, Amelia Zannoni, Chase A. Gonzalez, Peter Stringer, Roberto G. Fileni, Nitisha S. Gajavalli, Julia Langman, Grace K. Wong
Undergraduate Student Symposium
Understanding how psychedelic phenethylamines affect neural plasticity is essential in understanding effects associated with recreational drug use. Intracellular scaffolding proteins and downstream effectors, i.e., the microtubule cytoskeleton, have been proposed to modulate the effects of psychedelics. Microtubules are polymers of the protein tubulin that contribute to the cytoarchitecture, protein transport, and synaptic stability in cells. Our group has shown that psychedelic substituted phenethylamines interact with microtubules in a dose dependent manner to affect its ability to form. These interactions would prevent microtubule dependent protein transport within cells leading to altered cytoarchitecture of the cell and may be associated with adverse …
Assessment Of Brain Function And Soccer Penalty Kick Performance In Collegiate Soccer Players, Toniann Brezina
Assessment Of Brain Function And Soccer Penalty Kick Performance In Collegiate Soccer Players, Toniann Brezina
Undergraduate Student Symposium
BACKGROUND: The purpose of this research study is to assess National Collegiate Athletic Association (NCAA) men’s and women’s soccer players brain function while taking penalty kicks wearing an electroencephalogram (EEG) band and a heart rate monitor. An EEG is a device used to assess brain activity and will record four types of brain wave activities: alpha, beta, theta, and delta. Heart rate data provides additional insight during strenuous activity. Minimal research exists on EEG and soccer penalty kicks, but multiple published studies discuss high activity in the beta wave along with a low heart rate indicating a focused but relaxed …
Demonstrating Physical Control Over Others Changes One’S Sense Of Power: Testing A Novel Manipulation Of Physical Formidability, Tamara Bogdanova, Morgan Fala, Ned Vaitkevicius, Nissipriya Pill, Ariana Gonzalez
Demonstrating Physical Control Over Others Changes One’S Sense Of Power: Testing A Novel Manipulation Of Physical Formidability, Tamara Bogdanova, Morgan Fala, Ned Vaitkevicius, Nissipriya Pill, Ariana Gonzalez
Undergraduate Student Symposium
The aim of this study is to investigate the relationship between physical formidability and social power in modern humans. Previous research has shown that factors related to physical power, such as height, can affect a person’s perception of their own social power. We hypothesized that exercising physical power by engaging in the physical control of the behavior of another individual would affect people’s sense of their own social power and influence. To test this hypothesis, we employed a novel manipulation that used a human-to-human Transcutaneous Electrical Nerve Stimulation (TENS) device and a confederate to allow participants to feel as though …
Evaluating A Research Course Designed To Help Students Understand Protein Structure And Function, Omar Saleh, Jordan Nichole Carreras, Ryan Luib
Evaluating A Research Course Designed To Help Students Understand Protein Structure And Function, Omar Saleh, Jordan Nichole Carreras, Ryan Luib
Undergraduate Student Symposium
An educational curriculum has been implemented through the Farquhar Honors College (FHC) to integrate computational biology research for undergraduate students. Since its time as an independent study course, it has evolved into an Honors interdisciplinary course that is co-taught by professors from both the Departments of Biological Sciences and Chemistry & Physics with support from the NSF-funded Connecting Researchers, Educators, and STudents program, and 3D Molecular Designs. The course is intended to be applicable to FHC students regardless of their academic background. Throughout the Fall 2023 semester, peer mentors worked with student groups to assist in literature comprehension, protein selection, …
Evaluating The Association Between Undergraduate Major And Telehealth Utilization, Nimisha Kumar, Rojie Ekanayake, Wardah Lodhi
Evaluating The Association Between Undergraduate Major And Telehealth Utilization, Nimisha Kumar, Rojie Ekanayake, Wardah Lodhi
Undergraduate Student Symposium
Telehealth is a rapidly advancing phenomenon that utilizes technology to provide long-distance healthcare services to patients. The convenience of telehealth services is essential during emergency situations. For instance, the COVID-19 pandemic resulted in increased awareness and reliance of telehealth services worldwide. Additionally, telehealth provides time-saving crisis intervention amongst the recent worsening of the fentanyl and opioid crisis. Telehealth’s increased flexibility and convenience benefits populations facing barriers to healthcare access. As telehealth rapidly evolves, it is crucial to understand the factors that influence its adoption. The purpose of this study is to evaluate any associations between undergraduate students’ majors and telehealth …
Evaluation Of The Effects Of Mdmx And Mdm2 Inhibitors Causing Cell Death In Prostate Cancer Cells, Saaketh Palli, Revanth Madamsetty
Evaluation Of The Effects Of Mdmx And Mdm2 Inhibitors Causing Cell Death In Prostate Cancer Cells, Saaketh Palli, Revanth Madamsetty
Undergraduate Student Symposium
Objective: In this research, we examined the molecular mechanisms by which inhibition of the MDM2 and MDMX pathways induces cell death in prostate cancer cells. Background: In the United States alone, more than 3 million men are living with prostate cancer. Though significant advances have been made in treating prostate cancer, the need for new anti-cancer drugs and treatment methods still persists to fight this deadly disease. Therefore, our study explored the intracellular link between MDMX and necroptosis, to determine how manipulating this molecular target can lead to cell cycle arrest and cell death. Methods: The LNCaP Prostate Cancer cells …
How The Molecular Vibrations Of Water May Prevent Skin Cancer, Jarlynn Ramirez, Angelina Georg, Colette Costello, Mahi Desai, Sandeep Dronavalli
How The Molecular Vibrations Of Water May Prevent Skin Cancer, Jarlynn Ramirez, Angelina Georg, Colette Costello, Mahi Desai, Sandeep Dronavalli
Undergraduate Student Symposium
Studies have shown that when DNA absorbs ultraviolet light it can become structurally damaged leading to a mutation and possibly skin cancer. However, exposing skin to sunlight rarely leads to a mutation. This indicates that after the ultraviolet light is absorbed by the DNA the energy from the light can either cause a structural change to the DNA (a mutation) or be dissipated in a way that does not damage the DNA. Absorption of ultraviolet light causes molecular vibrations in DNA and these vibrations are what likely leads to structural changes in the DNA. One way the structural changes could …
Influence Of Growth Efficiency, Bacterial Density, And Metabolism On Antibiotic Resistance In Staphylococcus Aureus, Shreeya Patel, Estefania Marin Meneses
Influence Of Growth Efficiency, Bacterial Density, And Metabolism On Antibiotic Resistance In Staphylococcus Aureus, Shreeya Patel, Estefania Marin Meneses
Undergraduate Student Symposium
Antibiotic resistance presents a formidable challenge in medicine, with rising mortality rates and escalating hospital expenses attributed to infections caused by antibiotic-resistant bacteria. To address this critical issue, comprehending the mechanisms of bacterial resistance is paramount to the development of innovate drugs and treatment strategies. One such mechanism is the inoculum effect (IE), where the initial bacterial population density determines the minimum concentration of antibiotic required to eliminate the population. While previous work has shown that for gram negative bacteria, the relationship between ATP production and growth rate can account for the IE. However, it remains unclear as to if …
Learning From Louisiana: How Legislation And Location Affect Lgbtq+ Youth Mental Health, Abhay Dharanikota
Learning From Louisiana: How Legislation And Location Affect Lgbtq+ Youth Mental Health, Abhay Dharanikota
Undergraduate Student Symposium
Queer youth are disproportionately vulnerable to mental health challenges including elevated risks of social isolation and suicide ideation. Disparities in mental health outcomes among queer students exist across states, but the underlying factors contributing to these variations remain unclear. This research aims to understand why certain states exhibit better mental health outcomes among queer youth than others by analyzing nationwide LGBTQ+ mental health statistics. This knowledge can then inform effective programs and approaches to improving outcomes in other states. It is hypothesized that states offering accessible mental health services, safe spaces such as Gender and Sexuality Alliances (GSAs), and LGBTQ-affirming …
Long-Term Outcomes Of Covid-19 Survivors With Pre-Existing Dementia, Roham Hadidchi
Long-Term Outcomes Of Covid-19 Survivors With Pre-Existing Dementia, Roham Hadidchi
Undergraduate Student Symposium
It is known that patients with dementia are at higher risk of SARS-CoV-2 infection due to older age and may be susceptible to worse acute outcomes. Whether or not acute COVID-19 infection predisposes patients with pre-existing dementia to worse longer-term outcomes is unknown and few studies compare COVID-19 survivors with non-COVID controls. This retrospective study evaluated 9,806 patients with dementia in the Montefiore Health System in the Bronx (January 2016 to July 2023), which serves a large minority population in the epicenter of the pandemic. Patients who had pre-existing dementia at the time of COVID-19 infection (COVID-19 group) or the …
Measuring Drug Metabolism Efficiency Of P450 Enzyme, Rachel Gruboy, Kena Patel
Measuring Drug Metabolism Efficiency Of P450 Enzyme, Rachel Gruboy, Kena Patel
Undergraduate Student Symposium
Cytochrome P450 enzyme is responsible for the majority of drug metabolism in the human body, generally increasing the drugs’ solubility for secretion. Drug companies have routinely tested how fast drugs are metabolized and the metabolites they produce, but the low efficiency of drug metabolism makes this measurement a challenging task. The purpose of this project was to increase metabolism efficiency by changing the reaction conditions. The drug metabolism of cytochrome P450 enzyme was measured using commonly prescribed medications. The P450 enzyme uses NADPH to reduce the heme iron- O2 complex making it highly reactive. This highly reactive center is …
Modeling Cysteinyl Leukotriene Receptor Antagonist Knw For Possible Optimized Asthma Treatment, Sreejani Jonnalagadda, Shalet James
Modeling Cysteinyl Leukotriene Receptor Antagonist Knw For Possible Optimized Asthma Treatment, Sreejani Jonnalagadda, Shalet James
Undergraduate Student Symposium
Under-diagnosed and under-treated, particularly in low- and middle-income countries, asthma has affected 262 million people globally in 2019. Cysteinyl leukotriene receptors (Cys-LTRs) are a δ-branch of class A G protein-coupled receptors associated with physiological functions in airways with allergic inflammation. Current antiasthmatic medications such as pranlukast inhibit CysLT1R, yet many patients still do not respond to this drug. To better understand this process, the related receptor CysLT2R has been identified as a promising drug target for not only asthma but also other conditions such as brain injury and cancer. CysLT1R is associated with bronchoconstriction, inflammation, and mucus production in the …
Changes In Neuromuscular Function Across A Competitive Collegiate Cross-Country Season, Riley Schemel, Alexis Arena, Isabella Vargas-Blanchard
Changes In Neuromuscular Function Across A Competitive Collegiate Cross-Country Season, Riley Schemel, Alexis Arena, Isabella Vargas-Blanchard
Undergraduate Student Symposium
Resistance training strengthens neural adaptations that aid in improving neuromuscular functions such as the rate of force development (RFD) and power execution during an explosive task like a countermovement jump (CMJ). Distance runners do not engage in heavy resistance training and the effects of endurance running on neuromuscular functions are less known but may provide crucial biomarkers for monitoring performance and injury. The purpose of this intervention study was to evaluate changes in neuromuscular function from 7 weeks of competitive distance running without heavy resistance training. Thirteen university runners (age, 19.8±2.2 yrs; height, 1.74±0.10 m; mass, 61.9±9.0 kg) performed 3 …
Exploring Dysphagia In Alzheimer's Disease: A Comprehensive Meta-Analysis Literature Review, Katheline Hernandez
Exploring Dysphagia In Alzheimer's Disease: A Comprehensive Meta-Analysis Literature Review, Katheline Hernandez
Undergraduate Student Symposium
Alzheimer's disease and dementia are progressive conditions that develop gradually, potentially heightening the risk of dysphagia, a condition characterized by difficulties in swallowing. As Alzheimer's disease advances, it can impact cognitive and motor functions, potentially causing challenges in coordinating the muscles involved in the swallowing process. The aim of this literature review was to investigate and determine the prevalence of dysphagia among individuals with Alzheimer's disease and dementia, identify symptoms associated with dysphagia associated with Alzheimer's disease and dementia, explore relevant dysphagia interventions employed by healthcare professionals, and pinpoint common assessments used to identify both dementia and dysphagia. The systematic …
Interactions Between Atp Production, Growth Rate, And Antibiotic Lethality Determine The Inoculum Effect In Β-Lactamase-Expression Escherichia Coli, Ariane Kalifa, Philopatier Ibrahim, Kielan Singh
Interactions Between Atp Production, Growth Rate, And Antibiotic Lethality Determine The Inoculum Effect In Β-Lactamase-Expression Escherichia Coli, Ariane Kalifa, Philopatier Ibrahim, Kielan Singh
Undergraduate Student Symposium
Antibiotic resistance is a global threat, claiming approximately five million lives annually and impacting nations worldwide. In the U.S.A., this issue is significant, with 2.8 million diagnosed cases leading to 35,000 deaths each year. One way that bacteria can resist antibiotic treatment is through the inoculum effect (IE). IE explains how the concentration of antibiotics required to kill a population of bacteria increases as a function of bacterial density. Recently it was found that increasing ATP production/growth rate decreases the strength of IE. However, we do not know if this relationship can predict IE in bacteria that express β-lactamases as …
Mille-Feuille Magazine Littéraire, Printemps 2024, Pascale-Anne Brault
Mille-Feuille Magazine Littéraire, Printemps 2024, Pascale-Anne Brault
Mille-Feuille Magazine Littéraire
No abstract provided.
The Chicago Southland International Film Festival Kicks Off At Govstate, Office Of Marketing And Communications
The Chicago Southland International Film Festival Kicks Off At Govstate, Office Of Marketing And Communications
Newsroom
No abstract provided.
Hira Gene Mutation In Arabidopsis Via Crispr, Kelly Chen, Claire Shippy, Tara Phelps-Durr
Hira Gene Mutation In Arabidopsis Via Crispr, Kelly Chen, Claire Shippy, Tara Phelps-Durr
SACAD: Scholarly Activities
The purpose of this study is to mutate the Histone Repressor A (HIRA) gene in a mustard plant (Arabidopsis) using CRISPR technology. HIRA is a chromatin-remodeling protein that is required for proper development in both plants and animals. Creating new changes (mutations) in HIRA will help us better understand the mechanism of how HIRA regulates transcription, which is important for understanding developmental disorders in animals, as well as how improper development leads to a reduction in crop yields in plants
Social Media Does Not Elicit A Physiological Stress Response As Measured By Heart Rate And Salivary Cortisol Over 20-Minute Sessions Of Cell Phone Use, Suzanne Oppenheimer, Laura Bond, Charity Smith
Social Media Does Not Elicit A Physiological Stress Response As Measured By Heart Rate And Salivary Cortisol Over 20-Minute Sessions Of Cell Phone Use, Suzanne Oppenheimer, Laura Bond, Charity Smith
Biomedical Research Institute Publications and Presentations
The pervasive use of social media has raised concerns about its potential detrimental effects on physical and mental health. Others have demonstrated a relationship between social media use and anxiety, depression, and psychosocial stress. In light of these studies, we examined physiological indicators of stress (heart rate to measure autonomic nervous system activation and cortisol to assess activity of the hypothalamic-pituitary-adrenal axis) associated with social media use and investigated possible moderating influences of sex, age, and psychological parameters. We collected physiological data from 59 subjects ranging in age from 13 to 55 across two cell phone treatments: social media use …
April 3, 2024 (Vol. 108, No. 25), Daily Eastern News
April 3, 2024 (Vol. 108, No. 25), Daily Eastern News
Daily Eastern News: 2023-2024
No abstract provided.
Where Have All The Flowers Gone? A Call For Federal Leadership In Deer Management In The United States, Bernd Blossey, Darragh Hare, Donald M. Waller
Where Have All The Flowers Gone? A Call For Federal Leadership In Deer Management In The United States, Bernd Blossey, Darragh Hare, Donald M. Waller
Aspen Bibliography
Forests in the United States continue to lose biodiversity and many fail to regenerate due to high deer (family Cervidae) abundance. Declines in biodiversity and overall ecosystem health due to high deer populations increases prevalence of wildlife and human diseases associated with increasing tick abundances and decreases forest resilience and the ability to deliver benefits provided by healthy ecosystems. In the eastern and midwestern United States, white-tailed deer (Odocoileus virginianus) are the main stressor, while in the western U.S. elk (Cervus elaphus) and black-tailed and mule deer (Odocoileus hemionus) can become equally problematic. Federal …
Navigating Murky Waters: State-Level Strategies For Wetland Preservation And Tile Drainage Regulation After Sackett V. Epa, Caleb M. Swanson
Navigating Murky Waters: State-Level Strategies For Wetland Preservation And Tile Drainage Regulation After Sackett V. Epa, Caleb M. Swanson
Honors Thesis
Wetlands are some of the world’s most valuable ecosystems, serving as provisioners of species habitat, carbon sequestration, flood mitigation, water quality purification, and other ecosystem services. Human development has resulted in substantial wetland loss the world over. In the 1970s, the United States Congress passed the Clean Water Act, giving the EPA broad authority over wetland protection. However, in the summer of 2023, the United States Supreme Court decided Sackett v. EPA, limiting the EPA’s jurisdiction over wetlands to those indistinguishably connected to generally recognized “Waters of the United States” and removing federal protection for millions of acres of wetlands, …
Antiobesity And Antidyslipidemic Properties Of Clitoria Ternatea Petals Aqueous Extract Against Rats Induced By High-Fat Diet, I Gede Aswin Parisya Sasmana, Desak Made Wihandani, I Gede Krisna Arim Sadeva, Wilson Halim, Putu Putri Agustini, Lalita Samala, I Gusti Ngurah Agung Surya Pratama, Lidiya Nuraliza Rachmawati, Aizar Vesa Prasetyo, Ni Komang Ayu Amanda Setiari Jaya
Antiobesity And Antidyslipidemic Properties Of Clitoria Ternatea Petals Aqueous Extract Against Rats Induced By High-Fat Diet, I Gede Aswin Parisya Sasmana, Desak Made Wihandani, I Gede Krisna Arim Sadeva, Wilson Halim, Putu Putri Agustini, Lalita Samala, I Gusti Ngurah Agung Surya Pratama, Lidiya Nuraliza Rachmawati, Aizar Vesa Prasetyo, Ni Komang Ayu Amanda Setiari Jaya
Turkish Journal of Medical Sciences
Background/aim: Obesity is a chronic metabolic disease involving dysregulation of fat metabolism that affects 13% of the world’s population. Obesity has been linked to dyslipidemia with a lot of complication, including stroke, chronic kidney disease, fatty liver disease, and so on. One of the natural resources that have several potential effects including anticholesterol, antiobesity, and antidyslipidemia is the butterfly pea (Clitoria ternatea/CT). CT’s petal has been found to contain high levels of anthocyanins and tannins that can inhibit the biosynthesis of cholesterol and lipid. This study aims to investigate the antiobesity and antidyslipidemic effects of Clitoria ternatea extract (CTE).Materials and …
The Effect Of Human Recombinant Epidermal Growth Factor On Neovascularization And Pedicle Division Time In A Rat Interpolation Flap Model, Aziz Parspanci, Yavuz Keçeci̇, Peyker Temi̇z
The Effect Of Human Recombinant Epidermal Growth Factor On Neovascularization And Pedicle Division Time In A Rat Interpolation Flap Model, Aziz Parspanci, Yavuz Keçeci̇, Peyker Temi̇z
Turkish Journal of Medical Sciences
Background/aim: In practice, waiting 2–3 weeks for interpolation flaps pedicle division result in certain morbidities and discomfort for patient. The division time of flap pedicle depends on neovascularization from the recipient bed and includes wound healing stages. We aimed to investigate the effect of recombinant human epidermal growth factor (rhEGF) on the flap viability during early pedicle division.Materials and methods: Thirty-six rats were allocated to two main groups as control and study. A cranial based flap measuring 5 × 5 cm was elevated from the back, including all layers of the skin. While the cranial half of the defect was …
Harnessing Rna Technology To Advance Therapeutic Vaccine Antigens Against Chagas Disease, Chiara Mancino, Jeroen Pollet, Assaf Zinger, Kathryn M Jones, Maria José Villar, Ana Carolina Leao, Rakesh Adhikari, Leroy Versteeg, Rakhi Tyagi Kundu, Ulrich Strych, Federica Giordano, Peter J Hotez, Maria Elena Bottazzi, Francesca Taraballi, Cristina Poveda
Harnessing Rna Technology To Advance Therapeutic Vaccine Antigens Against Chagas Disease, Chiara Mancino, Jeroen Pollet, Assaf Zinger, Kathryn M Jones, Maria José Villar, Ana Carolina Leao, Rakesh Adhikari, Leroy Versteeg, Rakhi Tyagi Kundu, Ulrich Strych, Federica Giordano, Peter J Hotez, Maria Elena Bottazzi, Francesca Taraballi, Cristina Poveda
Center for Medical Ethics and Health Policy Staff Publications
Chagas disease (CD) (American trypanosomiasis caused by Trypanosoma cruzi) is a parasitic disease endemic in 21 countries in South America, with increasing global spread. When administered late in the infection, the current antiparasitic drugs do not prevent the onset of cardiac illness leading to chronic Chagasic cardiomyopathy. Therefore, new therapeutic vaccines or immunotherapies are under development using multiple platforms. In this study, we assessed the feasibility of developing an mRNA-based therapeutic CD vaccine targeting two known T. cruzi vaccine antigens (Tc24—a flagellar antigen and ASP-2—an amastigote antigen). We present the mRNA engineering steps, preparation, and stability of the lipid …