Digital Commons Network^™

Nanobodies In The Fight Against Infectious Diseases: Repurposing Nature's Tiny Weapons, Dina Mohamed Moustafa, Soha S. Rizk, Shahira A. Elbanna, Hanzada Tawfik Nour El-Din, Ahmed Sherif Attia

Dentistry

Nanobodies are the smallest known antigen-binding molecules to date. Their small size, good tissue penetration, high stability and solubility, ease of expression, refolding ability, and negligible immunogenicity in the human body have granted them excellence over conventional antibodies. Those exceptional attributes of nanobodies make them promising candidates for various applications in biotechnology, medicine, protein engineering, structural biology, food, and agriculture. This review presents an overview of their structure, development methods, advantages, possible challenges, and applications with special emphasis on infectious diseases-related ones. A showcase of how nanobodies can be harnessed for applications including neutralization of viruses and combating antibiotic-resistant bacteria …

The Influence Of Stage Of Ripeness On Consumer Perceptions Regarding Selected Aspects Of Non-Astringent Asian Persimmons (Diospyros Kaki Thunb), Robert A. Mirabello Jr

LSU Doctoral Dissertations

Asian Persimmon (Diospyros kaki Thunb.) is an important fruit crop in Asia and in recent decades has experienced expanding production around the world. Non-astringent cultivars are highly desirable in world markets. In the United States the Asian Persimmon is lesser known and consumed although it can be successfully grown in California and the Southeastern United States.This novel study examined the physical/chemical properties of the non-astringent Asian Persimmon ‘Jiro’ grown in Southern Louisiana at three distinct stages of ripeness described as Green (mid-September harvest), Light Orange (mid-October harvest) and Dark Orange (mid-November harvest). Data collected included color, weight, size, soluble …

Characteristics Influencing Enrollment Choices: A Mixed Methods Analysis Of Factors That Influence Black Students' Decision To Enroll In College Of Agriculture Programs, Jennifer L. Brown

LSU Doctoral Dissertations

This study aims to identify the barriers that affect Minority enrollment in agriculture, particularly focusing on increasing the enrollment of Black students. There is a significant concern regarding the gap between Black individuals and agriculture enrollment in post-secondary agriculture programs at land-grant institutions. The purpose of this mixed method phenomenology was to (1) identify factors that influence the decision of Black students to enroll in the College of Agriculture and (2) uncover the societal and economic influences that continue to serve as barriers to Black students’ enrolling in agriculture. The quantitative portion of the investigation utilized a web-based instrument structured …

The Cure For World Peace: A Public Health Strategy, Eva Eleftheriadis

Undergraduate Honors Theses

Health and the environment impact the security and stability of the global community, as can be seen through the effects of pandemics and climate change. This paper attempts to address these factors by helping create a preventative strategy for countries to be better equipped to face these challenges, and decrease the extent of future manifestations. Starting with a comparative analysis of the Black Death and the COVID-19 pandemic, this paper explores the relationship that exists between health, the environment, and human behavior. The analysis suggests that when these aspects are mutually beneficial, there is an increase in human security, which …

Age-Specific Induction Of Mutant P53 Drives Clonal Hematopoiesis And Acute Myeloid Leukemia In Adult Mice, Rasoul Pourebrahim, Rafael Heinz Montoya, Hiroki Akiyama, Lauren Ostermann, Shayuan Khazaei, Muharrem Muftuoglu, Natalia Baran, Ran Zhao, Tom Lesluyes, Bin Liu, Joseph D Khoury, Mihai Gagea, Peter Van Loo, Michael Andreeff

Faculty, Staff and Student Publications

The investigation of the mechanisms behind p53 mutations in acute myeloid leukemia (AML) has been limited by the lack of suitable mouse models, which historically have resulted in lymphoma rather than leukemia. This study introduces two new AML mouse models. One model induces mutant p53 and Mdm2 haploinsufficiency in early development, showing the role of Mdm2 in myeloid-biased hematopoiesis and AML predisposition, independent of p53. The second model mimics clonal hematopoiesis by inducing mutant p53 in adult hematopoietic stem cells, demonstrating that the timing of p53 mutation determines AML vs. lymphoma development. In this context, age-related changes in hematopoietic stem …

Selective Refueling Of Car T Cells Using Ada1 And Cd26 Boosts Antitumor Immunity, Yue Hu, Abhijit Sarkar, Kevin Song, Sara Michael, Magnus Hook, Ruoning Wang, Andras Heczey, Xiaotong Song

Faculty, Staff and Student Publications

Chimeric antigen receptor (CAR) T cell therapy is hindered in solid tumor treatment due to the immunosuppressive tumor microenvironment and suboptimal T cell persistence. Current strategies do not address nutrient competition in the microenvironment. Hence, we present a metabolic refueling approach using inosine as an alternative fuel. CAR T cells were engineered to express membrane-bound CD26 and cytoplasmic adenosine deaminase 1 (ADA1), converting adenosine to inosine. Autocrine secretion of ADA1 upon CD3/CD26 stimulation activates CAR T cells, improving migration and resistance to transforming growth factor β1 suppression. Fusion of ADA1 with anti-CD3 scFv further boosts inosine production and minimizes tumor …

Recent Findings On The Effectiveness Of Peer Support For Patients With Type 2 Diabetes, James J. Werner, Kelsey Ufholz, Prashant Yamajala

Faculty Scholarship

Purpose of Review: To review randomized controlled trials (RCTs) published from 2021–2023 that reported the effects of peer support interventions on outcomes in patients with type 2 diabetes (T2DM). Recent Findings: Literature searches yielded 137 articles and nine RCTs were ultimately reviewed. The reviewed trials involved in-person support groups, peer coach/mentor support, cultural peer support by community health workers, peer support during shared medical appointments (SMAs) including virtual reality-based SMAs, telehealth-facilitated programs, and telephone peer support. Most interventions combined two or more peer support strategies. Peer support was associated with significant decreases in HbA1c in 6 of the 9 reviewed …

Volume 122, Number 6 - Tuesday, May 21st, 2024, Saint Mary's College Of California

The Collegian

No abstract provided.

Academic Senate - Agenda, 5/21/2024, Academic Senate

Academic Senate Agendas

No abstract provided.

Abundance, Distribution, And Diversity Of The Paralarval Cephalopod Community In The Northern Gulf Of Mexico, Shannon Leah Riley

USF Tampa Graduate Theses and Dissertations

The abundance, distribution, and diversity of cephalopod paralarvae from the upper water column (0-130 meters) in the northern Gulf of Mexico (GOM) was studied using samples collected during six ichthyoplankton surveys between 2009 and 2012. A total of 2240 cephalopod paralarvae belonging to 21 families were examined. Octopodidae, Enoploteuthidae, and Ommastrephidae were the most abundant taxa collected in the time series. Five taxa had significantly different abundances between some sampling periods, while no significant differences in abundance occurred between any GOM regions (northwest GOM shelf, north-central GOM shelf, West Florida Shelf, GOM slope, and GOM basin). The majority of taxa …

The 2019 Raikoke Eruption As A Testbed Used By The Volcano Response Group For Rapid Assessment Of Volcanic Atmospheric Impacts, Jean Paul Vernier, Thomas J. Aubry, Claudia Timmreck, Anja Schmidt, Lieven Clarisse, Fred Prata, Nicolas Theys, Andrew T. Prata, Graham Mann, Hyundeok Choi, Simon Carn, Richard Rigby, Susan C. Loughlin, John A. Stevenson

Michigan Tech Publications

The 21 June 2019 Raikoke eruption (48°N, 153°E) generated one of the largest amounts of sulfur emission to the stratosphere since the 1991 Mt. Pinatubo eruption. Satellite measurements indicate a consensus best estimate of 1.5Tg for the sulfur dioxide (SO2) injected at an altitude of around 14-15km. The peak Northern Hemisphere (NH) mean 525gnm stratospheric aerosol optical depth (SAOD) increased to 0.025, a factor of 3 higher than background levels. The Volcano Response (VolRes) initiative provided a platform for the community to share information about this eruption which significantly enhanced coordination efforts in the days after the eruption. A multi-platform …

Invasiveness Of Black-Fungal (Mucormycosis) Disease Among Patients Suffering From Covid-19: Review, Zainab Sahib Abuhanen Quraish

Muthanna Medical Journal

A collection of moldy filamentous belonging to the orders Zygomycetes additionally Mucorales produce the infection known as mucormycosis. A frequent name for mucormycosis is "black fungus disease." This infection primarily affects diabetics and people with impaired immune systems. Inhaling mold containing industrial oxygen increases the risk of mucormycosis while COVID-19 infection lowers patients' immunity. The main goal of this review is to supply a thorough synopsis of COVID-19, recognize the essential fungal agent, and illustrate the Invasiveness and morbid manifestation as complications of COVID-19. During 2023, a thorough literature search was conducted using keywords like "Mucormycosis", "Blackfungus," "Mucorales," and "Zygomycetes," …

Cloverbud County Record, Christine Whiting

UNH Cooperative Extension

No abstract provided.

Ciliary Tip Actin Dynamics Regulate Photoreceptor Outer Segment Integrity, Roly Megaw, Abigail Moye, Zhixian Zhang, Fay Newton, Fraser Mcphie, Laura C Murphy, Lisa Mckie, Feng He, Melissa K Jungnickel, Alex Von Kriegsheim, Peter A Tennant, Chloe Brotherton, Christine Gurniak, Alecia K Gross, Laura M Machesky, Theodore G Wensel, Pleasantine Mill

Faculty, Staff and Students Publications

As signalling organelles, cilia regulate their G protein-coupled receptor content by ectocytosis, a process requiring localised actin dynamics to alter membrane shape. Photoreceptor outer segments comprise an expanse of folded membranes (discs) at the tip of highly-specialised connecting cilia, into which photosensitive GPCRs are concentrated. Discs are shed and remade daily. Defects in this process, due to mutations, cause retinitis pigmentosa (RP). Whilst fundamental for vision, the mechanism of photoreceptor disc generation is poorly understood. Here, we show membrane deformation required for disc genesis is driven by dynamic actin changes in a process akin to ectocytosis. We show RPGR, a …

Police-Made Law, Brenner M. Fissell

Faculty Publications

This Article presents evidence that police are writing laws that they enforce. This newly discovered phenomenon compounds the existing understanding of police “making” law through the exercise of discretion. They make law in a far more direct way, functioning as quasi-legislators at the local level—identifying a social problem, drafting an offense to address it, and directly proposing their offense for enactment. The conduct targeted, and the reasons for doing so, are diverse. For example, in one city a police chief successfully criminalized public intoxication so that intoxicated people would go to jails instead of hospitals; in another, a chief pushed …

Graded Decisions In The Human Brain, Tao Xie, Markus Adamek, Hohyun Cho, Matthew A Adamo, Anthony L Ritaccio, Jon T Willie, Peter Brunner, Jan Kubanek

2020-Current year OA Pubs

Decision-makers objectively commit to a definitive choice, yet at the subjective level, human decisions appear to be associated with a degree of uncertainty. Whether decisions are definitive (i.e., concluding in all-or-none choices), or whether the underlying representations are graded, remains unclear. To answer this question, we recorded intracranial neural signals directly from the brain while human subjects made perceptual decisions. The recordings revealed that broadband gamma activity reflecting each individual's decision-making process, ramped up gradually while being graded by the accumulated decision evidence. Crucially, this grading effect persisted throughout the decision process without ever reaching a definite bound at the …

An Unexpected Path For Malat1 In Neurons: Trafficking Out Of The Nucleus For Translation, Bradley W Wright, Jeremy E Wilusz

Faculty, Staff and Students Publications

The Malat1 (metastasis-associated lung adenocarcinoma transcript 1) long noncoding RNA is highly and broadly expressed in mammalian tissues, accumulating in the nucleus where it modulates expression and pre-mRNA processing of many protein-coding genes. In this issue of Genes & Development, Xiao and colleagues (doi:10.1101/gad.351557.124) report that a significant fraction of Malat1 transcripts in cultured mouse neurons are surprisingly exported from the nucleus. These transcripts are packaged with Staufen proteins in RNA granules and traffic down the lengths of neurites. They then can be released in a stimulus-dependent manner to be locally translated into a microprotein that alters neuronal gene …

Targeting The Substrate For Atrial Fibrillation: Jacc Review Topic Of The Week, Mark D Mccauley, Gianluca Iacobellis, Na Li, Stanley Nattel, Jeffrey J Goldberger

Faculty, Staff and Students Publications

The identification of the pulmonary veins as a trigger source for atrial fibrillation (AF) has established pulmonary vein isolation (PVI) as a key target for AF ablation. However, PVI alone does not prevent recurrent AF in many patients, and numerous additional ablation strategies have failed to improve on PVI outcomes. This therapeutic limitation may be due, in part, to a failure to identify and intervene specifically on the pro-fibrillatory substrate within the atria and pulmonary veins. In this review paper, we highlight several emerging approaches with clinical potential that target atrial cardiomyopathy-the underlying anatomic, electrical, and/or autonomic disease affecting the …

Conference Speaker Bios, Dusty Layton, Angela Williams

Conference Documents

No abstract provided.

Commencement Program [Spring 2024], Clark University

Commencement Programs

Annual Commencement Program for graduation ceremonies at Clark University, held on May 20th, 2024.

Environmental Damage Is A War Crime: Analyzing The Legal Implications Of The Russian Armed Invasion's Environmental Impact On Ukraine, Iryna Rekrut

JCLC Online

As a result of the armed invasion of Ukraine by the Russian

military, Ukraine has suffered extreme environmental damage that

affects both its land and its people. This article explores the

intersection of international law and environmental protection in the

context of armed conflicts, with a specific focus on the Russian armed

invasion of Ukraine. After describing the devastation faced by

Ukraine, this article examines existing frameworks in international

law such as the Rome Statute, the Geneva Conventions, customary

international humanitarian law, and domestic law. This overview

highlights guidelines in these frameworks that render environmental

damage during war impermissible. Despite …

Comprehensive In Vitro And In Vivo Evaluation Of Therapeutic Potential Of Bacopa-Derived Asiatic Acid Against A Human Oral Pathogen Streptococcus Mutans, Rajendran Jeyasri, Pandiyan Muthuramalingam, Arumugam Priya, Rajaiah Alexpandi, N. R. Siva Shanmugam, Saminathan Nivetha, Hyunsuk Shin, Shunmugiah Karutha Pandian, Arumugam Veera Ravi, Manikandan Ramesh

Food for Health: Publications

Dental caries is a common human oral disease worldwide, caused by an acid-producing bacteria Streptococcus mutans. The use of synthetic drugs and antibiotics to prevent dental caries has been increasing, but this can lead to severe side effects. To solve this issue, developing and developed countries have resorted to herbal medicines as an alternative to synthetic drugs for the treatment and prevention of dental caries. Therefore, there is an urgent need for plant-derived products to treat such diseases. Bacopa monnieri, a well-documented medicinal plant, contains 52 phytocompounds, including the pentacyclic triterpenoid metabolite known as asiatic acid (ASTA). Hence, …

"Branching Out: Examining Plant Cognition And Sentience", Steven Hansch, Leslie Barcus

Tales of WellBeing

New research on plant behavior and communication raises questions about plant awareness and sentience.

Nextflow Pipeline For Visium And H&E Data From Patient-Derived Xenograft Samples., Sergii Domanskyi, Anuj Srivastava, Jessica Kaster, Haiyin Li, Meenhard Herlyn, Jill C Rubinstein, Jeffrey Chuang

Faculty Research 2024

We designed a Nextflow DSL2-based pipeline, Spatial Transcriptomics Quantification (STQ), for simultaneous processing of 10x Genomics Visium spatial transcriptomics data and a matched hematoxylin and eosin (H&E)-stained whole-slide image (WSI), optimized for patient-derived xenograft (PDX) cancer specimens. Our pipeline enables the classification of sequenced transcripts for deconvolving the mouse and human species and mapping the transcripts to reference transcriptomes. We align the H&E WSI with the spatial layout of the Visium slide and generate imaging and quantitative morphology features for each Visium spot. The pipeline design enables multiple analysis workflows, including single or dual reference genome input and stand-alone image …

Detecting The Effect Of Genetic Diversity On Brain Composition In An Alzheimer's Disease Mouse Model., Brianna Gurdon, Sharon C Yates, Gergely Csucs, Nicolaas E Groeneboom, Niran Hadad, Maria Telpoukhovskaia, Andrew R Ouellette, Tionna Ouellette, Kristen M S O'Connell, Surjeet Singh, Thomas J Murdy, Erin Merchant, Ingvild Bjerke, Heidi Kleven, Ulrike Schlegel, Trygve B Leergaard, Maja A Puchades, Jan G Bjaalie, Catherine C Kaczorowski

Faculty Research 2024

Alzheimer's disease (AD) is broadly characterized by neurodegeneration, pathology accumulation, and cognitive decline. There is considerable variation in the progression of clinical symptoms and pathology in humans, highlighting the importance of genetic diversity in the study of AD. To address this, we analyze cell composition and amyloid-beta deposition of 6- and 14-month-old AD-BXD mouse brains. We utilize the analytical QUINT workflow- a suite of software designed to support atlas-based quantification, which we expand to deliver a highly effective method for registering and quantifying cell and pathology changes in diverse disease models. In applying the expanded QUINT workflow, we quantify near-global …

Taming The "Dartmouth Animal", Kira Lynn Parrish-Penny

Sociology Undergraduate Senior Theses

Reflecting on Dartmouth College’s tumultuous early years of coeducation in the 1970s, former president John Kemeny remarked that several students “took it on as a sort of mission to make life miserable for women students when they first arrived… from verbal abuse to … raids on women’s dormitories and… doing everything possible that a minority of students can do to make the women feel unwelcome.” Using archival data from the Rauner Special Collections Library, this thesis explores two questions: (1) how were norms and values around masculinity constructed at Dartmouth? (2) How did this construction of masculinity shift over the …

Condition Scoring Of Sheep, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Condition scoring sheep is an easy and accurate method of estimating the condition or 'nutritional wellbeing' of your sheep flock. It requires an assessment of the amount of muscle and fat covering the backbone and the short ribs of each sheep. This gives a picture of the sheep’s store of energy. It is the best method for monitoring pregnant and lactating ewes but is also useful for monitoring the growth of weaners.

Indigenous Land Back In Malaysia: Capturing Orang Asal Resistance Through #Kembalikantanahadat, Tarchithaa Chandra Sekharan

Senior Theses

The rise of a pan-indigenous network has been empowered by digital media in the 21st century. Universal concepts such as “landback” have traveled beyond the boundaries of language and borders. For indigenous peoples in Malaysia, the Orang Asal, online resistance is a reclamation of space long suppressed historically and physically. With actions like spreading local news on land rights court cases and development projects, indigenous reclamation from within the Orang Asal as well as indigenous allyship from citizens and diasporic Malaysians alike is possible. #KembalikanTanahAdat in Malay, #ReturnCustomaryLand is one of the many hashtags for a move towards increasing awareness …

Systemic Inflammatory Markers Of Persistent Cerebral Edema After Aneurysmal Subarachnoid Hemorrhage, Nadia Khan, Magdalena Kurnik-Łucka, Gniewomir Latacz, Krzysztof Gil

Faculty, Staff and Student Publications

In the first part of this article, the role of intestinal epithelial tight junctions (TJs), together with gastrointestinal dopaminergic and renin-angiotensin systems, are narratively reviewed to provide sufficient background. In the second part, the current experimental data on the interplay between gastrointestinal (GI) dopaminergic and renin-angiotensin systems in the regulation of intestinal epithelial permeability are reviewed in a systematic manner using the PRISMA methodology. Experimental data confirmed the copresence of DOPA decarboxylase (DDC) and angiotensin converting enzyme 2 (ACE2) in human and rodent enterocytes. The intestinal barrier structure and integrity can be altered by angiotensin (1-7) and dopamine (DA). Both …

Revealing Microbial Community Composition And Disinfection Efficacy Evaluation In Wholesale Pork Markets Through 16s Rdna Sequencing, Miao He, Lingyan Ma, Wentao Lv, Yingping Xiao, Hua Yang, Xiofan Wang, Jiangchao Zhao, Hangzhen Lan, Wen Wang, Xingning Xiao

Animal Science Faculty Publications and Presentations

This study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfectant was assessed by comparing the bacterial communities in the viable samples before and after disinfection. The bacterial abundances in meat, chopping boards, and knives were positively correlated (r > 0.95, p <  .05), suggesting potential cross-contamination risks during market transactions. The species richness indices for the external transport vehicles, pork hooks, and floors were significantly different from those of the samples taken after decontamination (p <  .05). Linear discriminant analysis Effect Size (LEfSe) analyses showed that Firmicutes, Proteobacteria, and Chloroflexi were significantly more abundant in the transaction period, whereas Bacteroidetes levels decreased compared to the clean-up period. NaClO disinfection …